WO2022033035A1

WO2022033035A1 - Two-dimensional material-based cardiac pacemaker connecting wire and preparation method therefor

Info

Publication number: WO2022033035A1
Application number: PCT/CN2021/082187
Authority: WO
Inventors: 闵永刚; 刘荣涛; 张诗洋; 朋小康; 黄兴文; 王勇; 刘屹东; 廖松义
Original assignee: 广东工业大学; 东莞华南设计创新院
Priority date: 2020-08-11
Filing date: 2021-03-22
Publication date: 2022-02-17
Also published as: CN111785446A

Abstract

A two-dimensional material-based cardiac pacemaker connecting wire and a preparation method therefor. The preparation method comprises the following steps: step S1, using a two-dimensional material as the core and a degradable polymer as the shell to prepare composite nanofibers by a coaxial electrospinning method; and step S2, drying the obtained composite nanofibers at 30-80℃ in vacuum, and then forming the two-dimensional material-based cardiac pacemaker connecting wire by a twisting process. The prepared two-dimensional material-based cardiac pacemaker connecting wire has good electrical conductivity and mechanical properties, can be degraded into small molecules in a human body and then discharged out of the body, does not generate inflammations, is non-toxic, and is more convenient to use; in addition, the preparation method is simple, and synthesis conditions are mature.

Description

A two-dimensional material-based cardiac pacemaker connecting wire and preparation method thereof

technical field

The invention relates to the technical field of biomedical materials, in particular to a two-dimensional material-based cardiac pacemaker connecting wire and a preparation method thereof.

Background technique

At present, the external power lead of the pacemaker is made of metal material, which needs to be removed after the patient recovers. The traditional method has some problems that cannot be ignored in terms of safety, antibacterial property and patient tolerance.

On the premise of ensuring electrical conductivity, the biodegradable nanofibers of two-dimensional materials can realize the active degradation of the connecting line after the patient is cured, and have antibacterial and anti-inflammatory properties without causing damage to the body. It has the advantages of good electrical conductivity, good biocompatibility, high mechanical strength, degradable and antibacterial.

SUMMARY OF THE INVENTION

In order to solve the technical problem that the existing cardiac pacemaker connecting line is unfavorable to use, the present invention provides a two-dimensional material-based cardiac pacemaker connecting line and a preparation method thereof.

The invention provides a preparation method of a two-dimensional material-based cardiac pacemaker connecting wire, comprising the following steps:

In step S1, the two-dimensional material is used as the core, and the degradable polymer is used as the shell, and the composite nanofibers are obtained after preparation by a coaxial electrospinning method;

Step S2, after vacuum drying the obtained composite nanofibers at 30-80° C., a two-dimensional material-based cardiac pacemaker connecting wire is formed through a twisting process.

Further, the two-dimensional material is one or more of GO, Mxene and black phosphorus BP; the degradable polymer is one or more of PLA, PCL, PLGA and PEG.

Further, the mass ratio of the two-dimensional material to the degradable polymer is (0.01-1):1.

Further, the following steps are also included before step S1:

In step S11, the two-dimensional material and the degradable polymer are respectively dissolved in a solvent.

Further, the solvent is in methanol, ethanol, ether, acetone, dichloromethane, chloroform, dimethyl sulfoxide, toluene, tetrahydrofuran, N,N-dimethylformamide, benzoic acid and N-methylpyrrolidone one or more of.

Further, the mass concentration of the conductive polymer after being dissolved in the solvent is 0.1-50%; the mass concentration of the degradable polymer after being dissolved in the solvent is 0.1-50%.

On the other hand, the present invention also provides a two-dimensional material-based cardiac pacemaker connecting wire, the two-dimensional material-based cardiac pacemaker connecting wire is prepared by using the preparation method.

The beneficial effects of the invention are as follows: the two-dimensional material-based cardiac pacemaker connecting wire prepared by the invention has good electrical conductivity and good mechanical properties, can be degraded into small molecules in the human body and can be excreted from the body, has no inflammation and is non-toxic, and is more convenient use; in addition, the preparation method of the present invention is simple, and the synthesis conditions are mature. On the premise of ensuring the stable electrical conductivity in the pH environment of the body fluid, the invention can realize the characteristics of good biocompatibility, high mechanical strength and simple preparation method of the composite nanofiber.

Description of drawings

FIG. 1 is a schematic diagram of the preparation process of the cardiac pacemaker connecting wire with a two-dimensional material base according to the present invention.

Figure 2 is an enlarged view of the PLA/PANI composite nanofibers prepared by the present invention.

detailed description

The following describes in detail the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary, and are intended to explain the present invention and should not be construed as limiting the present invention.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", " Rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", etc. The relationship is based on the orientation or positional relationship shown in the drawings, only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore It should not be construed as a limitation of the present invention.

In addition, the terms "first" and "second" are only used for descriptive purposes, and should not be construed as indicating or implying relative importance or implying the number of indicated technical features. Thus, a feature defined as "first" or "second" may expressly or implicitly include one or more of that feature. In the description of the present invention, "plurality" means two or more, unless otherwise expressly and specifically defined.

In the present invention, unless otherwise expressly specified and limited, terms such as "installation", "connection", "connection", "fixation" and other terms should be understood in a broad sense, for example, it may be a fixed connection or a detachable connection , or integrally connected; it can be a mechanical connection or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or the internal communication between the two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

In the present invention, unless otherwise expressly specified and limited, a first feature "on" or "under" a second feature may include the first and second features in direct contact, or may include the first and second features Not directly but through additional features between them. Also, the first feature being "above", "over" and "above" the second feature includes the first feature being directly above and obliquely above the second feature, or simply means that the first feature is level higher than the second feature. The first feature is "below", "below" and "below" the second feature includes the first feature being directly below and diagonally below the second feature, or simply means that the first feature has a lower level than the second feature.

The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.

As shown in FIG. 1 to FIG. 2 , the present invention provides a preparation method of a two-dimensional material-based cardiac pacemaker connecting wire, including the following steps:

Step S2 , after vacuum drying the obtained composite nanofibers at 30-80° C., a two-dimensional material-based cardiac pacemaker connecting wire is formed through a twisting process.

The two-dimensional material-based cardiac pacemaker connecting wire prepared by the invention has good electrical conductivity and good mechanical properties, can be degraded into small molecules in the human body and can be excreted, has no inflammation and is non-toxic, and is more convenient to use; The method is simple and the synthesis conditions are mature. On the premise of ensuring the stable electrical conductivity in the pH environment of the body fluid, the invention can realize the characteristics of good biocompatibility, high mechanical strength and simple preparation method of the composite nanofiber.

In an optional embodiment, the two-dimensional material is one or more of GO, Mxene and black phosphorus BP; the degradable polymer is one or more of PLA, PCL, PLGA and PEG. The mass ratio of the two-dimensional material to the degradable polymer is (0.01-1):1.

Specifically, the GO is graphene oxide (graphene oxide), which is an oxide of graphene. The oxygen-containing functional groups on the GO increase to make the properties more active than graphene. It can be improved by various reactions with oxygen-containing functional groups. nature.

MXenes are a class of two-dimensional inorganic compounds in materials science. These materials consist of transition metal carbides, nitrides or carbonitrides several atomic layers thick. It was originally reported in 2011 that MXene materials possess the metallic conductivity of transition metal carbides due to the presence of hydroxyl groups or terminal oxygens on the surface.

Black phosphorus BP is Black Phosphorus, a two-dimensional nanomaterial; black phosphorus is a corrugated layered crystal similar to graphite, which is easy to be exfoliated into single-layer or few-layer nanosheets. Black phosphorene is a natural p-type direct bandgap semiconductor, the bandgap can be adjusted from ~0.3eV (bulk) to ~1.5eV (monolayer) by the number of layers, and it has obvious anisotropy with high Electron mobility.

Polylactic acid (PLA) is a new type of bio-based and renewable biodegradable material, which is made from starch raw materials proposed by renewable plant resources (such as corn, cassava, etc.). Starch raw material is saccharified to obtain glucose, and then fermented from glucose and certain strains to produce high-purity lactic acid, and then chemical synthesis method is used to synthesize polylactic acid with a certain molecular weight. It has good biodegradability, and can be completely degraded by microorganisms in nature under specific conditions after use, and finally generates carbon dioxide and water, which does not pollute the environment, which is very beneficial to protect the environment and is recognized as an environmentally friendly material.

PCL, or polycaprolactone, is used as a drug release system.

Polycaprolactone has good biodegradability, biocompatibility and non-toxicity, and is widely used as medical biodegradable material and drug controlled release system. It can be used in tissue engineering and has been used as a drug sustained release system.

Poly(lactic-co-glycolic acid) (PLGA) is randomly polymerized from two monomers, lactic acid and glycolic acid. It is a degradable functional polymer organic compound with good properties. It has excellent biocompatibility, non-toxicity, good encapsulation and film-forming properties, and is widely used in pharmaceutical, medical engineering materials and modern industrial fields.

Polyethylene glycol (PEG) is non-toxic, non-irritating, slightly bitter in taste, has good water solubility, and has good compatibility with many organic components. They have excellent lubricity, moisture retention, dispersibility, adhesive, antistatic agent and softener, etc. They are used in cosmetics, pharmaceuticals, chemical fibers, rubber, plastics, papermaking, paints, electroplating, pesticides, metal processing and food processing, etc. There are a wide range of applications in the industry.

In an optional embodiment, the following steps are further included before step S1:

Further, the solvent is in methanol, ethanol, ether, acetone, dichloromethane, chloroform, dimethyl sulfoxide, toluene, tetrahydrofuran, N,N-dimethylformamide, benzoic acid and N-methylpyrrolidone one or more of. The mass concentration of the conductive polymer after being dissolved in the solvent is 0.1-50%; the mass concentration of the degradable polymer after being dissolved in the solvent is 0.1-50%.

The two-dimensional material-based cardiac pacemaker connecting wire prepared by the invention has good electrical conductivity, good mechanical properties, can be degraded into small molecules in the human body and can be excreted, has no inflammation and is non-toxic, and is more convenient to use; The method is simple and the synthesis conditions are mature. On the premise of ensuring the stable electrical conductivity in the pH environment of the body fluid, the invention can realize the characteristics of good biocompatibility, high mechanical strength and simple preparation method of the composite nanofiber.

Specific examples are as follows:

Example 1

1. Preparation: Dissolve 1g GO in 10g N,N-dimethylformamide and add it to syringe 1 in Figure 1; 4.5g PLA particles are dissolved in 70% dichloromethane, 30% N,N-dimethylformamide The amide mixed solvent was dissolved and added to the syringe 2 in Figure 1; the coaxial electrospinning voltage was set to 20kV, the ejection speed was 0.008ml/min, and the distance was 15cm. The PLA/GO composite nanofibers with the core of PANI and the shell of PLA were obtained, which were vacuum dried at 45°C overnight, and turned into a pacemaker connecting wire after twisting.

2. Performance test: The obtained PLA/GO connecting wire has a Young's modulus of 15.6 MPa, a breaking strength of 1.5 MPa, and a breaking elongation of 86.7% after the mechanical property test, which meets the mechanical requirements of the connecting wire; the electrical conductivity is 5.45 S/cm ; Good cell adhesion, 7d cell activity reached 3.4, higher than the blank group; 21d degradation was 32.8%; anti-Gram bacteria reached 85.6%.

Example 2

1. Preparation: Dissolve 1.5g of MXene in 20g of N-methylpyrrolidone and add it to syringe 1 in Figure 1; 5g of PCL particles are dissolved in 15g of dichloromethane and added to syringe 2 in Figure 1 after dissolving; the settings are the same as The shaft electrospinning voltage was 25kV, the ejection speed was 0.005ml/min, and the distance was 20cm. The PCL/MXene composite nanofibers with MXene as the core and PCL as the shell were obtained, which were vacuum dried at 50°C overnight, and turned into a cardiac pacemaker connecting wire after twisting process.

2. Performance test: The obtained PCL/MXene connecting wire has a Young's modulus of 10.8 MPa, a breaking strength of 6.64 MPa, and a breaking elongation of 72.5% after the mechanical property test, which meets the mechanical requirements of the connecting wire; the electrical conductivity is 7.25 S/cm ; Good cell adhesion, 7d cell activity reached 1.88, higher than the blank group; 21d degradation was 32.8%, anti-Gluconococcus to 69%.

In the description of this specification, reference is made to the description of the terms "one embodiment", "some embodiments", "one embodiment", "some embodiments", "example", "specific example", or "some examples", etc. It is intended that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The above content is a further detailed description of the present invention in conjunction with specific embodiments, and it cannot be considered that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art to which the present invention pertains, some simple deductions or substitutions can be made without departing from the concept of the present invention.

Claims

A preparation method of a two-dimensional material-based cardiac pacemaker connecting wire, characterized in that it comprises the following steps:

In step S1, the two-dimensional material is used as the core, and the degradable polymer is used as the shell, and the composite nanofibers are obtained after preparation by a coaxial electrospinning method;

Step S2, after vacuum drying the obtained composite nanofibers at 30-80° C., a two-dimensional material-based cardiac pacemaker connecting wire is formed through a twisting process.
The preparation method of a two-dimensional material-based cardiac pacemaker connecting wire according to claim 1, wherein the two-dimensional material is one or more of GO, Mxene and black phosphorus BP; The degrading polymer is one or more of PLA, PCL, PLGA and PEG.
The method for preparing a two-dimensional material-based cardiac pacemaker connecting wire according to claim 1, wherein the mass ratio of the two-dimensional material to the degradable polymer is (0.01-1):1.
The method for preparing a two-dimensional material-based cardiac pacemaker connecting wire according to claim 1, characterized in that, before step S1, it further comprises the following steps:

In step S11, the two-dimensional material and the degradable polymer are respectively dissolved in a solvent.
The preparation method of a two-dimensional material-based cardiac pacemaker connecting wire according to claim 4, wherein the solvent is methanol, ethanol, ether, acetone, dichloromethane, chloroform, dimethyl sulfoxide , one or more of toluene, tetrahydrofuran, N,N-dimethylformamide, benzoic acid and N-methylpyrrolidone.
The method for preparing a two-dimensional material-based cardiac pacemaker connecting wire according to claim 4, wherein the mass concentration of the conductive polymer dissolved in the solvent is 0.1-50%; the degradable polymer is dissolved in the solvent The mass concentration of 0.1 to 50%.
A two-dimensional material-based cardiac pacemaker connecting wire, characterized in that the two-dimensional material-based cardiac pacemaker connecting wire is prepared by the preparation method according to any one of claims 1 to 6 .