WO2022026824A3 - Inht30 transgenic soybean - Google Patents

Inht30 transgenic soybean Download PDF

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Publication number
WO2022026824A3
WO2022026824A3 PCT/US2021/043897 US2021043897W WO2022026824A3 WO 2022026824 A3 WO2022026824 A3 WO 2022026824A3 US 2021043897 W US2021043897 W US 2021043897W WO 2022026824 A3 WO2022026824 A3 WO 2022026824A3
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WO
WIPO (PCT)
Prior art keywords
inht30
transgenic soybean
plants
transgenic
soybean
Prior art date
Application number
PCT/US2021/043897
Other languages
French (fr)
Other versions
WO2022026824A2 (en
Inventor
Michael Lee NUCCIO
Michael Andreas Kock
Joshua L. Price
Original Assignee
Inari Agriculture Technology, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US17/248,936 external-priority patent/US11359210B2/en
Priority claimed from US17/249,640 external-priority patent/US11214811B1/en
Priority claimed from US17/302,121 external-priority patent/US11242534B1/en
Priority claimed from US17/302,110 external-priority patent/US20220030822A1/en
Priority claimed from US17/302,739 external-priority patent/US11326177B2/en
Priority claimed from US17/303,116 external-priority patent/US11369073B2/en
Priority claimed from PCT/US2021/043170 external-priority patent/WO2022026379A1/en
Priority to CA3188412A priority Critical patent/CA3188412A1/en
Priority to BR112023001834A priority patent/BR112023001834A2/en
Application filed by Inari Agriculture Technology, Inc. filed Critical Inari Agriculture Technology, Inc.
Publication of WO2022026824A2 publication Critical patent/WO2022026824A2/en
Publication of WO2022026824A3 publication Critical patent/WO2022026824A3/en
Priority to US18/058,144 priority patent/US20230083144A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Transgenic INHT30 soybean plants comprising modifications of the MON87708 soybean locus which provide for facile excision of the modified MON87708 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.
PCT/US2021/043897 2020-07-31 2021-07-30 Inht30 transgenic soybean WO2022026824A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BR112023001834A BR112023001834A2 (en) 2020-07-31 2021-07-30 TRANSGENIC SOYBEAN PLANT CELL, TRANSGENIC SOYBEAN PLANT PART, TRANSGENIC SOYBEAN PLANT, METHOD FOR OBTAINING A BULK POPULATION OF INDOGAMIC SEEDS, METHOD FOR OBTAINING HYBRID SOYBEAN SEED, DNA MOLECULE, PROCESSED TRANSGENIC SOYBEAN PLANT PRODUCT , BIOLOGICAL SAMPLE, NUCLEIC ACID MOLECULE ADAPTED FOR DETECTION OF GENOMIC DNA, METHOD FOR DETECTING A SOYBEAN PLANT CELL AND METHOD FOR EXCISION OF THE INHT30 TRANSGENIC LOCIE OF THE SOYBEAN PLANT GENOME
CA3188412A CA3188412A1 (en) 2020-07-31 2021-07-30 Inht30 transgenic soybean
US18/058,144 US20230083144A1 (en) 2020-07-31 2022-11-22 Inht30 transgenic soybean

Applications Claiming Priority (44)

Application Number Priority Date Filing Date Title
US202063059860P 2020-07-31 2020-07-31
US202063059916P 2020-07-31 2020-07-31
US202063059813P 2020-07-31 2020-07-31
US202063059963P 2020-07-31 2020-07-31
US63/059,813 2020-07-31
US63/059,916 2020-07-31
US63/059,860 2020-07-31
US63/059,963 2020-07-31
US202163199930P 2021-02-03 2021-02-03
US63/199,930 2021-02-03
US202163199951P 2021-02-04 2021-02-04
US202163199949P 2021-02-04 2021-02-04
US63/199,949 2021-02-04
US63/199,951 2021-02-04
US17/248,936 2021-02-12
US17/248,936 US11359210B2 (en) 2020-07-31 2021-02-12 INIR12 transgenic maize
US17/249,640 US11214811B1 (en) 2020-07-31 2021-03-08 INIR6 transgenic maize
US17/249,640 2021-03-08
US202163201029P 2021-04-09 2021-04-09
US202163201030P 2021-04-09 2021-04-09
US63/201,030 2021-04-09
US63/201,029 2021-04-09
US17/302,110 US20220030822A1 (en) 2020-07-31 2021-04-23 Inht26 transgenic soybean
US17/302,110 2021-04-23
US17/302,121 US11242534B1 (en) 2020-07-31 2021-04-23 INHT31 transgenic soybean
US17/302,121 2021-04-23
US17/302,739 US11326177B2 (en) 2020-07-31 2021-05-11 INIR12 transgenic maize
US17/302,739 2021-05-11
US17/303,116 2021-05-20
US17/303,116 US11369073B2 (en) 2020-07-31 2021-05-20 INIR12 transgenic maize
US202163202569P 2021-06-16 2021-06-16
US63/202,569 2021-06-16
US202163203137P 2021-07-09 2021-07-09
US63/203,137 2021-07-09
USPCT/US2021/043170 2021-07-26
USPCT/US2021/043207 2021-07-26
USPCT/US2021/043187 2021-07-26
PCT/US2021/043170 WO2022026379A1 (en) 2020-07-31 2021-07-26 Expedited breeding of transgenic crop plants by genome editing
USPCT/US2021/043161 2021-07-26
PCT/US2021/043207 WO2022026403A2 (en) 2020-07-31 2021-07-26 Removable plant transgenic loci with cognate guide rna recognition sites
PCT/US2021/043161 WO2022026375A1 (en) 2020-07-31 2021-07-26 Genome editing of transgenic crop plants with modified transgenic loci
USPCT/US2021/043192 2021-07-26
PCT/US2021/043192 WO2022026395A2 (en) 2020-07-31 2021-07-26 Excisable plant transgenic loci with signature protospacer adjacent motifs or signature guide rna recognition sites
PCT/US2021/043187 WO2022026390A1 (en) 2020-07-31 2021-07-26 Generation of plants with improved transgenic loci by genome editing

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US18/058,144 Continuation US20230083144A1 (en) 2020-07-31 2022-11-22 Inht30 transgenic soybean

Publications (2)

Publication Number Publication Date
WO2022026824A2 WO2022026824A2 (en) 2022-02-03
WO2022026824A3 true WO2022026824A3 (en) 2022-03-17

Family

ID=80036086

Family Applications (12)

Application Number Title Priority Date Filing Date
PCT/US2021/043483 WO2022026566A1 (en) 2020-07-31 2021-07-28 Inir17 transgenic maize
PCT/US2021/043468 WO2022026554A1 (en) 2020-07-31 2021-07-28 Inir12 transgenic maize
PCT/US2021/043440 WO2022026540A1 (en) 2020-07-31 2021-07-28 Inht26 transgenic soybean
PCT/US2021/043496 WO2022026574A1 (en) 2020-07-31 2021-07-28 Inir6 transgenic maize
PCT/US2021/043479 WO2022026563A1 (en) 2020-07-31 2021-07-28 Inht31 transgenic soybean
PCT/US2021/043933 WO2022026848A1 (en) 2020-07-31 2021-07-30 Inir20 transgenic soybean
PCT/US2021/043919 WO2022026841A2 (en) 2020-07-31 2021-07-30 Inir4 transgenic maize
PCT/US2021/043897 WO2022026824A2 (en) 2020-07-31 2021-07-30 Inht30 transgenic soybean
PCT/US2021/043945 WO2022026856A2 (en) 2020-07-31 2021-07-30 Inht27 transgenic soybean
PCT/US2021/043851 WO2022026801A1 (en) 2020-07-31 2021-07-30 Inir11 transgenic maize
PCT/US2021/043935 WO2022026849A1 (en) 2020-07-31 2021-07-30 Inir19 transgenic soybean
PCT/US2021/044198 WO2022026954A2 (en) 2020-07-31 2021-08-02 Inir6 transgenic maize

Family Applications Before (7)

Application Number Title Priority Date Filing Date
PCT/US2021/043483 WO2022026566A1 (en) 2020-07-31 2021-07-28 Inir17 transgenic maize
PCT/US2021/043468 WO2022026554A1 (en) 2020-07-31 2021-07-28 Inir12 transgenic maize
PCT/US2021/043440 WO2022026540A1 (en) 2020-07-31 2021-07-28 Inht26 transgenic soybean
PCT/US2021/043496 WO2022026574A1 (en) 2020-07-31 2021-07-28 Inir6 transgenic maize
PCT/US2021/043479 WO2022026563A1 (en) 2020-07-31 2021-07-28 Inht31 transgenic soybean
PCT/US2021/043933 WO2022026848A1 (en) 2020-07-31 2021-07-30 Inir20 transgenic soybean
PCT/US2021/043919 WO2022026841A2 (en) 2020-07-31 2021-07-30 Inir4 transgenic maize

Family Applications After (4)

Application Number Title Priority Date Filing Date
PCT/US2021/043945 WO2022026856A2 (en) 2020-07-31 2021-07-30 Inht27 transgenic soybean
PCT/US2021/043851 WO2022026801A1 (en) 2020-07-31 2021-07-30 Inir11 transgenic maize
PCT/US2021/043935 WO2022026849A1 (en) 2020-07-31 2021-07-30 Inir19 transgenic soybean
PCT/US2021/044198 WO2022026954A2 (en) 2020-07-31 2021-08-02 Inir6 transgenic maize

Country Status (3)

Country Link
BR (12) BR112023001798A2 (en)
CA (12) CA3188278A1 (en)
WO (12) WO2022026566A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240011042A1 (en) 2020-07-31 2024-01-11 Inari Agriculture Technology, Inc. Excisable plant transgenic loci with signature protospacer adjacent motifs or signature guide rna recognition sites
US11242534B1 (en) 2020-07-31 2022-02-08 Inari Agriculture Technology, Inc. INHT31 transgenic soybean

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CA3188408A1 (en) 2022-02-03
CA3188278A1 (en) 2022-02-03
WO2022026554A1 (en) 2022-02-03
BR112023001779A2 (en) 2023-02-23
CA3188275A1 (en) 2022-02-03
BR112023001845A2 (en) 2023-02-23
BR112023001821A2 (en) 2023-02-23
CA3188412A1 (en) 2022-02-03
WO2022026849A1 (en) 2022-02-03
WO2022026801A1 (en) 2022-02-03
BR112023001834A2 (en) 2023-02-23
WO2022026954A3 (en) 2022-03-10
CA3188413A1 (en) 2022-02-03
WO2022026856A2 (en) 2022-02-03
WO2022026540A1 (en) 2022-02-03
CA3188415A1 (en) 2022-02-03
BR112023001853A2 (en) 2023-02-23
CA3188277A1 (en) 2022-02-03
BR112023001778A2 (en) 2023-02-23
CA3188279A1 (en) 2022-02-03
BR112023001798A2 (en) 2023-02-23
WO2022026563A1 (en) 2022-02-03
BR112023001835A2 (en) 2023-02-23
BR112023001849A2 (en) 2023-02-23
CA3188323A1 (en) 2022-02-03
CA3188276A1 (en) 2022-02-03
WO2022026954A2 (en) 2022-02-03
WO2022026841A3 (en) 2022-03-03
BR112023001780A2 (en) 2023-02-23
BR112023001804A2 (en) 2023-02-23
WO2022026566A1 (en) 2022-02-03
BR112023001811A2 (en) 2023-02-23
WO2022026856A3 (en) 2022-03-10
WO2022026848A1 (en) 2022-02-03
CA3188441A1 (en) 2022-02-03
WO2022026574A1 (en) 2022-02-03
WO2022026841A2 (en) 2022-02-03
CA3188440A1 (en) 2022-02-03
WO2022026824A2 (en) 2022-02-03

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