WO2022003399A1

WO2022003399A1 - An herbal remedy for regulating liver enzymes and alcoholic fatty liver

Info

Publication number: WO2022003399A1
Application number: PCT/IB2020/056191
Authority: WO
Inventors: Khabat KHEDRI
Original assignee: Khedri Khabat
Priority date: 2020-06-30
Filing date: 2020-06-30
Publication date: 2022-01-06

Abstract

The invented herbal medicine has anti-cancer properties and can be used to treat acute liver failure, constipation, and better bowel function and at the same time is a natural antibacterial medicine. This product is made from four plants fennel flower, bitter Almond leaf, platanus and chicory leaf and two saps (Pistacia atlantica gum and honey) which have strong and active antioxidants and has a great influence on the liver and they are useful for fatty liver and at the same time can be useful for all types of hepatitis. This medicine can heal many liver disorders, including liver failure, liver cancer, and acute fatty liver.

Description

Title of invention : An herbal remedy for regulating liver enzymes and alcoholic fatty liver

Technical Field

[0001] The technical field of this invention relates to herbal medicine to treatment of liver failures, acute constipation, and peptic ulcer.

Background Art

[0002] In order to treat the mentioned diseases, there are many treatments most of which have a chemical origin. In this method, we have claimed an herbal medicine that has a new compound for this treatment.

[0003] The invention titled as PHARMACEUTICAL COMPOSITION FOR

PREVENTING OR TREATING LIVER CANCER CONTAINING MIXED HERBAL EXTRACT with the publication number 1020190076105 is available in the South Korean Patent Office, which is summarized as follows:

[0004] The present invention relates to a pharmaceutical composition for preventing or treating liver cancer containing a mixed herbal extract. More specifically, the present invention relates to the pharmaceutical composition for preventing or treating liver cancer containing a mixed extract of Rehmannia glutinosa, Cornus officinalis, and Dioscorea polystachya. The composition according to the present invention contains the mixed extract of Rehmannia glutinosa, Cornus officinalis, and Dioscorea polystachya as an active component, wherein the mixed extract can inhibit the growth of liver cancer cells, thereby being able to be usefully used as the active component of the composition for preventing, alleviating or treating liver cancer. In order to treat the mentioned diseases, there are many treatments most of which have a chemical origin. In this method, we have claimed an herbal medicine that has a new compound for this treatment.

[0005] The invention titled as PHARMACEUTICAL COMPOSITION FOR

PREVENTING OR TREATING LIVER CANCER CONTAINING MIXED HERBAL EXTRACT with the publication number 1020190076105 is available in the South Korean Patent Office, which is summarized as follows: [0006] The present invention relates to a pharmaceutical composition for preventing or treating liver cancer containing a mixed herbal extract. More specifically, the present invention relates to the pharmaceutical composition for preventing or treating liver cancer containing a mixed extract of Rehmannia glutinosa, Cornus officinalis, and Dioscorea polystachya. The composition according to the present invention contains the mixed extract of Rehmannia glutinosa, Cornus officinalis, and Dioscorea polystachya as an active component, wherein the mixed extract can inhibit the growth of liver cancer cells, thereby being able to be usefully used as the active component of the composition for preventing, alleviating or treating liver cancer.

[0007] The ingredients of this medicine are quite different from those in claimed one.

Summary of Invention

[0008] The herbal medicine usage has a long history, tracing back to ancient times and for a long time, has been served as the most important human tool to cure disease. Upon emerging modern medicine and the widespread use of chemical drugs, the tendency of people to use herbal medicine in some countries was reduced. But now, after about a century from the absolute domination of modern medicine, herbal medicine has been received a great deal of attention and the people have much tendency to use it.

[0009] The reasons behind the tendency of people to these plants include the enormous side effects of chemical drugs and the weakness of classical medicine in the treatment of chronic diseases. In the invention, by combining six medicinal plants, a medicine was produced to treat these diseases, as it prevents liver damage by having flavonoids and antioxidants and high phenolic compounds.

Technical Problem

[0010] The liver which plays a key role in the gastrointestinal process and the gastrointestinal system converts micronutrients into usable forms for the body.

[0011 ] The liver failures include a wide range of diseases and disorders that damage liver tissue or its function and mainly the liver tissue may be inflamed upon contact with a virus. [0012] The most important factors that cause diseases and disorders of the liver involve hepatitis A, B and C viruses, alcohol consumption, and excessive intake of anodynes. In addition, obesity causes the accumulation of fats in the liver.

[0013] AST-SGOT and ALT-SGOT are sensitive markers in various types of liver diseases. AST-SGOT is normally found in different types of tissues such as liver, heart, muscle, Kidney, brain.

[0014] This enzyme enters the bloodstream at the time of injury to any of these issues. For example, its serum concentration increases during heart attacks and muscle failures.

[0015] A major part of ALT-SGPT, in contrast to AST, is normally found in the liver. Although the enzyme is exclusively in the liver, the liver contains a very high concentration of this enzyme. This medicine lowers liver enzymes.

[0016] The enzyme enters the bloodstream as the result of the liver damage, so, the enzyme is relatively used as a special indicator of hepatic conditions.

[0017] Constipation or lack of regular bowel movements is a disease in the gastrointestinal system which results in solid feces in the colon and their excretion comes with pain and difficulty. Acute constipation may cause intestinal closure which in turn requires surgical intervention. The main causes of this complication may be a poor diet, hormonal imbalance, side effects of drugs, or the anatomy of the body among others.

Solution to Problem

[0018] The invented herbal medicine has anti-cancer properties and can be used to treat acute liver failure, constipation, and better bowel function and at the same time is a natural antibacterial medicine.

[0019] This product is made from four plants fennel flower, bitter Almond leaf, platanus and chicory leaf and two saps (Pistacia atlantica gum and honey) which have strong and active antioxidants and has a great influence on the liver and they are useful for fatty liver and at the same time can be useful for all types of hepatitis. This medicine can heal many liver disorders, including liver failure, liver cancer, and acute fatty liver. [0020] The substances and compounds of this medicine, as well as their properties, follow:

[0021] 1) Pistacia atlentica gum: in the local dialect, Larestani and Bastaki are called Boh, in Kurdi is

[0022] Called Daghahzawan or Darwan or Daraban.

[0023] A tree is a genus of pistachios which has three different varieties.

[0024] From this tree, three varieties grow in Iran:

[0025] Gum turpentine is a resin or concentrated sap of gum-tree or wild pistachio tree. Usually, the gum is secreted from the trunk of tree.

[0026] Naturally or by bringing a cut in the fall season which is called turpentine in Persian Language and it uses in bubble gum and it also has many health and industrial benefits.

[0027] Turpentine sap at the time of secretion from tree trunk was bright green and it is thick and sticky and it uses as a laxative to treat constipation. And because it was stomach tonic and it helps to digest food and it is good appetizing eminent scholars such as Abu Ali Sina used it for treating a series of gastrointestinal diseases. Turpentine like antibiotic somewhat confronts bacteria which is the main cause of ulcer and duodenal ulcers. Meanwhile, many native people eat raw sap of turpentine in fast mode because they believe that it results in removing kidney stones.

[0028] Turpentine sap is an excellent drug for stomach and liver varices, due to its specific compound. Turpentine can also help to improve the performance of the liver and spleen and its salve with saturated fat is useful for softening curved and dry nail. And the healing of ulcer in buttocks and its compound with olive oil is useful for removing skin swelling and cracking and itchy body.

[0029] The evaluation of the anti-bacterial effects of pistacia atlantica:

[0030] Since some plants like Pistacio atlantica has antibacterial property and can be used as a harmless alternative for antibiotics. Antibacterial effects of the Pistacio’s Essence was tested on pathogenic bacteria and were compared with the newest antibiotics that are recommended against such bacteria. [0031] Helicobacteria Pylori:

[0032] Essential oils and components activity of turpentine against H. pylori was evaluated after isolating insoluble polymers and its significant effect on the treatment of stomach and duodenal ulcer was observed.

[0033] The test results were as follows:

[0034] 50% of tested species were destroyed under a concentration of 125mg/ml of the essence and 90% of the species were killed subjected to a concentration of 500mg/ml so, Pistacio gum has a relatively good anti-bacterial activity against H. pylori bacteria.

[0035] Staphylococcus aureus, Escherichia coli and Colostridium sporogens bacteria can generally cause food intoxication and gastrointestinal inflammation in humans. The essence of the pistacio sap has been known as an antibacterial compound against most microorganisms.

[0036] According to the results, the alcoholic essence of pistacio sap on the bacteria not only showed a deterrent effect but also has antibacterial effect by studying the organoleptic effects of pistacio’s sap essence in food and hence it can be used as a protective substance.

[0037] Evaluation of anti-toxic effects for liver:

[0038] Researches have shown that Flavonoids of pistacio gum have hepatic protective activity. Also, the expression of essential enzymes in the production of fatty acids (Fas, Fatty acid synthase) with interference decreases significantly.

[0039] Upon the intervention of Flavonoids of pistacio gum on the liver cells, the activity of Acetyle-co-carboxylase (ACE) and deacyl-glycerd azyl trans feras (DGAT) enzyme is reduced in turn results in a 36% decrease in the content of VLDL triglycerides. The interference Flavonoids in the liver cells, there is a significant effect on the Activity of limiting enzyme of the cholesterol synthesis pathway, FIMG-Coareductase. Flavonoids in the liver cells resulting in the reduction of the compound of labeled Acetate (1 -14C) with fatty acid. The activity of Fas enzyme, unlike its expression, has not changed, but its substray Malonillocove decreased because it is the production of ACC enzyme. [0040] 2) Nigella sativa:

[0041] The Nigella sativa belongs to the order of Ranunculales and the family of Ranunculaceae.

[0042] Its seed has menstrual, anti-bacteria, laxative effects and increases milk secretion.

[0043] Chemical compounds of Nigella sativa:

[0044] The Nigellia sativa contains stable oil (35-40%), volatile oil (0.5-1%), protein (23%), different Amino acids, sugars, Mucilage, Alkaloids, organic acids, Tannins, resins, toxic glycosides, Metarbin, Saponins, Melanthigenin, Lipase phytosterols, vitamins (Such as vitamin C, Folic acid, Thiamine, Niacin) and a variety of minerals (Iron, Zinc, Calcium) the volatile oil of Nigella sativa produced substances such as Thymol. Tymukinon is one of the compounds which results in a major part of medical effects of Nigella sativa.

[0045] Antioxidant effects of Nigella sativa on the liver:

[0046] This plant improves the antioxidant capacity of tissues. In this regard, the Nigella sativa decreases the concentration of Nitric oxid and Malondial Dehyde in the brain and heart and increases the amount of Glutathione, antioxidant enzyme such as Glutathione Transferases and Catalase. It also increases the amount of Catalase, Superoxide, dismutase, and Glutathione Peroxidas in the liver and gastrointestinal system.

[0047] 3) Honey:

[0048] Chemical properties of honey:

[0049] Honey is fraught with various nutrients. Of course, these compounds differ in various types of it which are briefly described below:

[0050] Sugars: so far, about 20sugars have been identified in the honey which total amount of it is about 80% and Glucose (31%) and Fructose (38%) are the most important ones.

[0051] Enzyme: honey contains many enzymes, including Invertase, Diastase, Glucose Oxides and etc. [0052] Acidity or PH: honey is an acidic substance and its PH is different from 4.3 to 1.6.

[0053] Minerals: the honey contains a variety of minerals such as Calcium,

Potassium, Iron, Phosphorus Magnesium and etc. studies have shown that the more color of the honey is darker, it contains more minerals

[0054] Vitamins: the honey is rich in different vitamins which the most important of them are vitamins B1 , B2, B3, B5, B6, and vitamin C.

[0055] Honey is also effective in treating peptic ulcer. A teaspoon of natural honey in a fasting manner can prevent the growth of a dangerous bacterium Helicobacter which cause most peptic ulcers.

[0056] The honey kills antibiotic-resistant bacteria which cause pulmonary infection in patients who have Cystic Fibrosis in culture medium.

[0057] The honey (natural) pasture which produces Hydrogen Peroxide, prevents the growth of the bacteria. The honey prevents Saphylococcal and Intrathecal infection the bacteria which are resistance to strong antibiotics such as Methiciline, Vancomycin.

[0058] 4) Bitter almond leaf:

[0059] The bitter almond leaf contains a special substance (1 to 3%) which its scientific name is Amygdalin. Vitamin B17 or Amygdalin is a Cyanogen Glocosid which had an anti-cancer activity which is produced under the brand name of Laaetrile. The chemical structure of Amygdalin includes two molecules of Glucose, a Benzaaldhyde which Causes Anesthetic and a Hydrocyanic acid has been produced that is an anti-cancer Compound.

[0060] Activity of Amygdalin or vitamin B17:

[0061] Larryl and pure form of Amygdalin contains a substance which is decomposed into Cyanid when it comes into contact with the cells of the human body, but normal and healthy cells show natural defense against this process because healthy cells contain an enzyme called Rudens which cause the combination of cyanide Molecule and Sulfur. The combination of cyanide and Sulfur is called Cyanat which is a harmless Substance and is excreted through the urine from the body. The level of Cyanide Produce from the use of Latifill is so low and harmless that the body easily copes with it, but cancer cells are unable to perform any anti- Cyanide defense action, they contain b-Glucosidase enzyme which causes Laticyl to release both of Its compounds, namely Cyanide and Benzaldyhyde.

This powerful combination completely kills cancer cells. Unlike the conventional treatment of cancer, Leatri can kill cancer cells without damaging healthy cells.

[0062] 5) Platanus leaves:

[0063] Chemical compound:

[0064] Tannins and different types of phenolic compounds and some minerals such as Iron are abundantly found in the combination of plantain tree. The plantain leaf is very useful for the liver because of the presence of Phenolic compounds.

[0065] 6) Chicory leaf:

[0066] Chicory is a cold nature plant and it can obviate heat and inflammation of the body. The liver is a detox organ in which the metabolism of fat is one of the most important functions of this organ and if there is a disorder in this organ, it will affect all other organs of the body. Larryl and pure form of Amygdalin contains a substance which is decomposed into Cyanid when it comes into contact with the cells of the human body, but normal and healthy cells show natural defense against this process because healthy cells contain an enzyme called Rudens which cause the combination of cyanide Molecule and Sulfur. The combination of cyanide and Sulfur is called Cyanat which is a harmless Substance and is excreted through the urine from the body.

[0067] The level of Cyanide Produce from the use of Latifill is so low and harmless that the body easily copes with it, but cancer cells are unable to perform any anti- Cyanide defense action, they contain b-Glucosidase enzyme which causes Laticyl to release both of Its compounds, namely Cyanide and Benzaldyhyde.

[0068] 5) Platanus leaves:

[0069] Chemical compound: [0070] Tannins and different types of phenolic compounds and some minerals such as Iron are abundantly found in the combination of plantain tree. The plantain leaf is very useful for the liver because of the presence of Phenolic compounds.

[0071] 6) Chicory leaf:

[0072] Chicory is a cold nature plant and it can obviate heat and inflammation of the body. The liver is a detox organ in which the metabolism of fat is one of the most important functions of this organ and if there is a disorder in this organ, it will affect all other organs of the body.

[0073] According to research, the Chicory essence plays a significant role in the production of the liver cells and the source is rich in vitamin C and it rids all chronic liver diseases.

[0074] Important effects:

[0075] Chemical compounds in the leaf, in addition to a mineral such as Sulfate, Phosphate Sodium Magnesium, Potassium Nitrate, it contains Glucoside called Shikuain. The use of Chicory has been prevalent since ancient times and at least BC. One of the important uses of this plant which has been prevalent in the past, it has been used as a liver tonic and its use in liver disease. Nowadays it is also one of the most important uses of it in liver failure.

[0076] It also has effects such as stomach tonic, diuretic, blood purifier, obstructive jaundice, anti-fever (anti-pyretic), appetizing, ridding of urinary sediment, and eliminating anemia, of course, these cases are being evaluated and definitive new therapies will be anticipated in the near future. Choicory essence protects the liver from damage caused by obstructive cholestasis.

[0077] Medicine formula:

[0078] First, 20g of gum sap (turpentine) with 10g of Nigella sativa powder is mixed. Then stir up one minute then add 100g of natural honey to the mixture and stirred it up. Then we produce essence from mixing 50g of Choicory leaf and 50g of bitter almond Powder and 20g of plantain leaf with 200cc of Hydroalcoholic at 60C for 60minutes and add this essence to the above mixture and mix it as much as to is produced a homogeneous mixture. This medication should be taken three times daily after the main meal of breakfast, lunch, and dinner, each time it takes a tablespoon to treat all three diseases of liver failure, peptic ulcer, and acute constipation.

Advantageous Effects of Invention

[0079] 1) One of the main properties of the Nigella Sativa seed is that it strengthens the immune system.

[0080] 2) Is effective in phlegmatic fever and melancholy.

[0081] 3) It has a good effect on gastrointestinal system and intestinal parasites.

[0082] 4) It treats stomach tympanites and the pain caused by it.

[0083] 5) It is effective in preventing and peptic ulcer.

[0084] 6) It prevents chronic diseases and cancers because of the effect of Nigella Sativa Remains in the body for a long time.

Brief Description of Drawings Fig.1

[0085] [Fig.1 ] Product production process

Industrial Applicability

[0086] This product is made from four plants fennel flower, bitter Almond leaf, platanus and chicory leaf and two saps (Pistacia atlantica gum and honey) which have strong and active antioxidants and has a great influence on the liver and they are useful for fatty liver and at the same time can be useful for all types of hepatitis. This medicine can heal many liver disorders, including liver failure, liver cancer, and acute fatty liver.

Reference Signs List

[0087] 1- Gilani AH, Jabeen Q, Ullah khan MA. A review of medicinal uses and pharmacological activities of Nigella sativa. Pak J Biol Sci 2004; 7: 441 -51.

[0088] 2- Al-Hader A, Aqel M, Hasan Z. Hypoglycemic Effects of the Volatile Oil of Nigella sativa Seeds. Pharm Biol 1993; 31 : 96-100.

[0089] 3- Kaleem M, Kirmani D, Asif M, Ahmed Q, Bano B. Biochem effects of Nigella sativa L seeds in diabetic rats. Indian J Exp Biol 2006; 44: 745-8. [0090] 4- Pari L, Sankaranarayanan C. Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocin-nicotinamide induced diabetic rats. Life Sci 2009; 85: 830-4.

[0091] 5- Meral I, Yener Z, Kahraman T, Mert N. Effect of Nigella sativa on glucose concentration, lipid peroxidation, anti-oxidant defence system and liver damage in experimentally-induced diabetic rabbits. J Vet Med A Physiol Pathol Clin Med 2001 ; 48: 593-9.

[0092] 6- Fararh KM, Atoji Y, Shimizu Y, Takewaki T. Isulinotropic properties of Nigella sativa oil in Streptozotocin plus Nicotinamide diabetic hamster. Res Vet Sci 2002; 73: 279-82.

[0093] 7- El-Dakhakhney M, Mady N, Lembert N, Ammon HPT. The hypoglycemic effect of Nigella sativa oil is mediated by extrapancreatic actions. Planta Med 2002; 68: 465-6.

[0094] 8- Fararh KM, Atoji Y, Shimizu Y, Shiina T, Nikami H, Takewaki T.

Mechanisms of the hypoglycaemic and immunopotentiating effects of Nigella sativa L. oil in streptozotocin-induced diabetic hamsters. Res Vet Sci 2004; 77: 123-9.

[0095] 9- Hamdy NM, Taha RA. Effects of Nigella sativa oil and thymoquinone on oxidative stress and neuropathy in streptozotocin-induced diabetic rats.

Pharmacol 2009; 84: 127-34.

[0096] 10- Kanter M, Akpolat M, Aktas C. Protective effects of the volatile oil of Nigella sativa seeds on b-cell damage in streptozotocin-induced diabetic rats: a light and electron microscopic study. J Mol Hist 2009 ; 40 : 379-85.

[0097] 11- Rchid H, Chevassus H, Nmila R, Guiral C, Petit P, Choka^'iri M, Sauvaire Y. Nigella sativa seed extracts enhance glucose-induced insulin release from rat- isolated Langerhans islets. Fundam Clin Pharmacol 2004; 18: 525-9.

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[0105] 19- Zargari Ali, medical plants, fourth ed., Tehran, Tehran University Press, 1990, Vol 1 , p.4-43

Claims

1- An herbal medicine for the treatment of liver failures, constipation and peptic ulcers, which consists of the following components: a. gum sap (turpentine) -20 g b. Nigella sativa powder- 10 g c. natural honey- 100 g d. Choicory leaf poweder-20g e. bitter almond Powder-50g f. plantain leaf-20g g. Hydroalcoholic solvent-200cc

2- According to the claim 1, first, turpentine is mixed with Nigella sativa powder and subjects to stir up for one minute and then after addition of natural honey to the mixture, it is stirred up again, subsequently essence consisted of Choicory leaf and bitter almond and plantain leaf Powder with Hydroalcoholic at 60C for 60minutes is produced and add this essence is added to the above mixtures and is stirred up to obtain a homogeneous mixture and this can be prepared as an oral syrup or 500 mg tablets and taken according to the prescription.