WO2021221594A2 - Multi-probiotic oil combination containing borage oil and production method thereof - Google Patents

Abstract

The present invention particularly relates to a multi-probiotic and oil combination consisting of borage oil selected as fixed oil, which forms oily carriers, patchouli oil selected as essential oil, peppermint oil, myrrh oils with probiotic bacteria Akkermensia muciniphila, Lactobacillus gasseri, Bacillus clausii, Bacillus subtilis, Leuconostoc mesenteroides, Lactobacillus rhamnosus, Lactobacillus cameliae, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bacillus clausii, Lactococcus lactis, Streptococcus thermophilus developed to be used in health sector to be used in treatment and/or preventive treatments for osteoporosis regression, regression of menopause/andropause symptoms, prostaglandin/eicosanoid regulation, geriatric support and a production method thereof.

Description

MULTI-PROBIOTIC OIL COMBINATION CONTAINING BORAGE OIL AND PRODUCTION METHOD THEREOF

Field of the Invention

The present invention relates to a combination of multi-probiotic oils combination developed for use in health and food sector consumed as a food supplement and preventive food to be used as a therapeutic and/or inhibitor for osteoporosis regression, regression of menopause/ andropause symptoms, prostaglandin/ eicosanoid regulation, providing geriatric support.

The present invention particularly relates to a probiotic and oil combination consisting of borage oil, patchouli oil, peppermint oil, myrrh oil with probiotic bacteria Akkermensia muciniphila, Lactobacillus gasseri, Bacillus clausii, Bacillus subtilis, Leuconostoc mesenteroides, Lactobacillus rhamnosus, Lactobacillus cameliae, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bacillus clausii, Lactococcus lactis, Streptococcus thermophilus and a production method thereof.

State of the Art

Recently, as a result of the increased awareness of consumers, the trend regarding health protection by means of nutrition has increased. This condition has revealed the term functional food and its industry. Functional foods are shortly defined as "foods that positively affect health in addition to their nutritional value". Today, foods or supplements containing probiotics establish a substantial part of this sector.

Probiotics use nutrients by attaching to intestinal surfaces and creating an ecological environment for themselves and thus they restrict the development of pathogens by creating a competitive environment. Furthermore, they prevent the growth of other harmful bacteria by producing various organic acids such as acetic acid and lactic acid, lowering the pH, producing bacteriocins and hydrogen peroxide under certain conditions. The positive effect of probiotics is not limited to the gastrointestinal tract, but it is known that they also provide various nutritional and therapeutic effects. It increases the bioavailability of lipids and proteins by means of the enzymatic hydrolysis that occurs with the participation of these bacteria and they reduce the allergenic quality and effect of foodstuffs. For example, they can digest lactose and reduce the symptoms caused by lactose intolerance by lowering the level of lactose in fermented dairy products. Furthermore, probiotics are effective in lowering serum cholesterol level, in the treatment and prevention of diarrhea, in the control of Helicobacter pylori infection and inflammatory bowel disease, they also show various anticarcinogenic and antimutagenic effects. It has been reported that probiotics have positive effects in the healing process of wounds, and probiotic use in babies reduces diarrhea, colic pain, allergic diseases, and respiratory disorders and strengthens the immune system according to the findings.

Today, although aromatic oils and probiotic contents are used in health, food and cosmetics sectors, there are not enough studies regarding their use in combination. There are various dermo-cosmetic and odour treatment areas where aromatic oils are used. In this field, edible oils are consumed as food supplements depending on their rich content. Furthermore, they are also used in psychosocial cosmetic therapies due to its pleasant odour property. Probiotic ingredients are also widely consumed as food supplements. Some beneficial bacteria and/or yeast combinations are used as drugs for intestinal diseases. Flowever, although there are formulas available to prevent the formation of gastrointestinal (digestive system) disorders caused by nutritional habits of today, they were insufficient.

Borage oil, patchouli oil, peppermint oil, myrrh oil and selected probiotic combination are taken into account in the inhibitive and/or preventive approach proposed for osteoporosis regression, regression of menopause/ andropause symptoms, prostaglandin/ eicosanoid regulation, geriatric support.

The family of beneficial bacteria in the content of selected probiotic combination, the metabolic, immune system, osteoporosis and age-related diseases that occur in their deficiencies have been the subject of many studies. There are many studies regarding brain-intestinal line, but the treatments aimed at eliminating the deficiencies in this line have been limited to chemical approaches.

The following applications are encountered in the literature regarding the subject matter. Patent application numbered TR 2018/08116 with title "A new probiotic initiator culture for use in humans and animals" relates to a new probiotic initiator culture consisting of the lactic acid bacteria Streptococcus thermophilus. This invention relates to its use in the prevention and/or treatment of intestinal infections for use in humans and animals, and its use as an immunomodulator.

Patent application numbered TR 2019/07851 with the title “Lactobacillus rhamnosus bacteria for the treatment of for example bacterial vaginosis.” relates to a lactic acid Lactobacillus rhamnosus bacteria suitable for the treatment of bacterial vaginosis. The invention aims to provide the Lactobacillus rhamnosus strain with a dietetic or pharmaceutical composition for the treatment of vaginal and female genital and urogenital infections and/or urinary tract infections caused by Lactobacillus rhamnosus deficiency.

Another invention is the patent application numbered TR 2013/14095 with title "Preparation containing probiotic bacteria and fish oil". Proposed invention is a probiotic preparation consisting of a powder mixture containing Bifidobacterium Infantis, Bifidobacterium breve and LactobasHlus acidophilus probiotic bacteria, which is used by pouring vial liquid containing inulin and the proposed liquid in it, to be used in the elimination of probiotic deficiency and in the proper functioning of children's mental development, strengthening of nervous systems, eye development and energy metabolism, important property of this preparation is proposed vial liquid contains fish oil containing Omega-3 fatty acids.

Another invention is the patent application numbered TR 2018/11298 with the title "Pharmaceutical composition effective in skin diseases in the form of cream “ relates to a composition in the form of a cream containing shea butter oil, tea tree oil, frankincense resin, muffin oil, aloe vera oil, borage oil, myrtle oil, grape seed oil, wheat oil, red centaury oil, calendula flower oil. The invention is a natural pharmaceutical composition in the form of a cream for use in the treatment of skin diseases/conditions.

As a result, due to the abovementioned disadvantages and the insufficiency of the current solutions regarding the subject matter, a development is required to be made in the relevant technical field. Brief Description of the Invention

The present invention is related to multi probiotic and oil combination which fulfils the abovementioned requirements, eliminate all disadvantages and bring some additional advantages.

The main aim of the invention is to provide a probiotic and oil combination that is consumed as a supplementary and preventive food to be used as therapeutic and/or preventive treatment for providing osteoporosis regression, menopause/ anthropause symptoms regression, prostaglandin/eicosanoid regulation, geriatric support, is developed for use in health and food sector.

Another aim of the invention is to provide a probiotic and oil combination consisting of borage oil, patchouli oil, peppermint oil, myrrh oil with probiotic bacteria Akkermensia muciniphila, Lactobacillus gasseri, Bacillus clausii, Bacillus subtilis, Leuconostoc mesenteroides, Lactobacillus rhamnosus, Lactobacillus cameliae, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bacillus clausii, Lactococcus lactis, Streptococcus thermophilus.

Another aim of the invention is to be used as a therapeutic agent and as a supplement in preventive medicine in the preventive and/or inhibitive approach envisaged for increasing the ability to recover in case of inflammation or injury by inducing prostaglandin/eicosanoid synthesis in or after bone destruction (osteoporosis), sudden depressive states during andropause/menopause, various trauma, inflammation, disease, eliminating the immune system problems (geriatric symptoms) that develop in older ages.

Another aim of the invention is to provide a probiotic product that has no adverse side effects and does not contain harmful chemicals and contributes to human health.

In order to achieve the aforementioned aims, the present invention particularly relates to a multi-probiotic and oil combination consisting of borage oil selected as fixed oil, which forms oily carriers, patchouli oil selected as essential oil, peppermint oil, myrrh oils with probiotic bacteria Akkermensia muciniphila, Lactobacillus gasseri, Bacillus clausii, Bacillus subtilis, Leuconostoc mesenteroides, Lactobacillus rhamnosus, Lactobacillus cameliae, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bacillus clausii, Lactococcus lactis, Streptococcus thermophilus developed to be used in health and food sector to be used in inhibitive and/or preventive treatments for osteoporosis regression, regression of menopause/andropause symptoms, prostaglandin/eicosanoid regulation, geriatric support and a production method thereof.

The structural and characteristic features of the present invention will be understood clearly by the following detailed description and therefore the evaluation shall be made by taking the detailed description into consideration.

Detailed Description of the Invention

In this detailed description, the inventive multi-probiotic oil combination is described only for clarifying the subject matter in a manner such that no limiting effect is created.

The present invention particularly relates to a probiotic and oil combination consisting of borage oil selected as fixed oil, which forms oily carriers, patchouli oil as essential oil, peppermint oil, myrrh oils with probiotic bacteria Akkermensia muciniphila, Lactobacillus gasseri, Bacillus clausii, Bacillus subtilis, Leuconostoc mesenteroides, Lactobacillus rhamnosus, Lactobacillus cameliae, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bacillus clausii, Lactococcus lactis, Streptococcus thermophilus developed to be used in health and food sector to be used inhibitive and/or preventive treatments for osteoporosis regression, menopause/andropause symptoms regression, prostaglandin/eicosanoid regulation, geriatric support and a production method thereof.

Borage ( Borago officinalis) contains high amounts of gamma linolenic acid (GLA) and stearidonic acid (SDA), omega-3, and omega-6 as a type of polyunsaturated fatty acid (PUFA). It is also used in the treatment of eczema and skin diseases, in the treatment of inflammation, cancer diseases because of its antioxidant properties, as a pain reliever, infections, and in the treatment of respiratory tract diseases. Essential oils and aromatic extracts are used in the perfume and flavour industry, in perfumes, food additives, cleaning products, cosmetics and pharmaceutical preparations, as a source of aroma chemicals or as a starting source in the synthesis of nature-identical and semi-synthetic flavour preparations. Aromatherapy is particularly used for indigestion problems, skin diseases, headaches, insomnia, stress, cancer treatment, and improving mental perception. In addition to the antibacterial and antifungal effects of essential oils and aroma extracts, they have also effects on the nervous system, digestive system, immune system and respiratory system. Calendula oil, geranium oil and lemon oil used in health and cosmetic industries as an essential oil are used as essential oils.

Table 1. Inventive multi probiotic oil combination content

The present invention comprises the following in total mixture by weight; 95-97% borage oil, 0,22-0.24% patchouli oil, 0,21-0.23% mint oil, 0,23-0.25% myrrh oil, 0,150-0,250% Akkermensia muciniphila, 0,150-0,250% Lactobacillus gasseri, 0,150-0.250% Bacillus clausii, 0,150-0.250 Bacillus subtilis% 0,150-0,250%

Leuconostoc mesenteroides, 0,150-0.250% Lactobacillus rhamnosus 0,150- 0,250%Lactobacillus cameliae, 0,150-0, 250%Lactobacillus acidophilus

0,150-0,250% Lactobacillus bulgaricus, 0,150-0,250% Bifidobacterium animalis, 0,150-0,250% Bifidobacterium bifidum, 0,150-0, 25%0 Bifidobacterium longum, 0,150-0,250% Bifidobacterium infantis, 0,150-0,250% Bacillus clausii, 0,050- 0,150% Lactococcus lactis, 0,050-0,150% Streptococcus thermophilus .

A preferred embodiment of the invention comprises the following in total mixture by weight; 96,296% borage oil, 0,236% patchouli oil, 0,223% mint oil, 0,245% myrrh oil, 0,2% Akkermensia muciniphila, 0,2% Lactobacillus gasseri, 0,2% Bacillus clausii, 0,2% Bacillus subtilis, 0,2% Leuconostoc mesenteroides, 0,2% Lactobacillus rhamnosus, 0,2% Lactobacillus cameliae, 0,2% Lactobacillus acidophilus, 0,2% Lactobacillus bulgaricus, 0,2% Bifidobacterium animalis, 0,2% Bifidobacterium bifidum, 0,2% Bifidobacterium longum, 0,2% Bifidobacterium infantis, 0,2% Bacillus clausii, 0,1% Lactococcus lactis, 0,1% Streptococcus thermophilus .

The inventive probiotic and oil combination and production method comprises the following process steps;

- Isolating microorganisms in sterile area at 22°C - 45°C temperature, 35-75% humidity and 2 -12 days interval and purifying isolates, homogeneously mixing with trihalose, maltodextrin, platinose, cyclodextrin, dextrose and all kinds of 6-carbon sugar and all prebiotic fibers in the bioreactor,

- Freeze drying of microorganisms in a freezer-freezer lyophilizer in a sterile area for 1 - 5 days at a temperature between -80°C and -20°C,

- Mixing the microorganism combination in the cubic mixer,

- Adding patchouli, mint and myrrh oils into borage oil,

- Adding the following bacteria to oil and homogenizing the same in the range of 1-5 days, in the sterile area Akkermensia muciniphila, Lactobacillus gasseri, Bacillus clausii, Bacillus subtilis, Leuconostoc mesenteroides, Lactobacillus rhamnosus, Lactobacillus cameliae, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bacillus clausii, Lactococcus lactis, Streptococcus thermophilus , - Filling of probiotic and oil combination into amber glass bottles with a peristaltic pump

Filled bottles are kept for 72 hours at 25°C for physical control before packaging.

The most ideal working environment of the probiotic combination in the invention is the essential and fixed oil mixture as detailed above. These elements are the original and sustainable characteristics of the invention and have an important contribution to the invention process since they have not been made previously. It will be used as a therapeutic agent and supplement in preventive medicine in the inhibitive and/or preventive approach proposed for osteoporosis regression, menopause/ andropause symptoms regression, prostaglandin/ eicosanoid regulation, geriatric support.

It is prescribed that individuals who are allergic to any ingredient contained in the invention should not use it. It has no known side effects. It can be consumed 1-2 times a day in the range of 5-10 ml hungry/full. The product is recommended to be consumed in the afternoon. Any bulges, unusual odours and similar situations in the product shall be reported to the authorized persons. The invention can be used until the disorders are eliminated and subsequently can be used for prophylaxis (protective).

Claims

1. A multi-probiotic oil combination developed for osteoporosis regression, regression of menopause/andopause symptoms, prostaglandin/eicosanoid regulation, geriatric support, characterized by comprising; Akkermensia muciniphila, Lactobacillus gasseri, Bacillus clausii, Bacillus subtilis, Leuconostoc mesenteroides, Lactobacillus rhamnosus, Lactobacillus cameliae, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bacillus clausii, Lactococcus lactis, Streptococcus thermophilus probiotic bacteria and borage oil, patchouli oil, mint oil and myrrh oil.

2. The multi-probiotic oil combination according to claim 1 , characterized by comprising in total mixture by weight; 95-97% borage oil, 0,22-0,24% patchouli oil, 0,21-0,23% peppermint oil, 0,23-0,25% myrrh oil, 0,150-0,250% Akkermensia muciniphila, 0,150-0,250% Lactobacillus gasseri, 0,150-0,250% Bacillus clausii, 0,150-0,250% Bacillus subtilis, 0,150-0,250% Leuconostoc mesenteroides, 0,150-0,250% Lactobacillus rhamnosus 0,150-0,250% Lactobacillus cameliae, 0,150-0,250% Lactobacillus acidophilus, 0,150-0,250% Lactobacillus bulgaricus, 0,150-0,250% Bifidobacterium animalis, 0,150-0,250% Bifidobacterium bifidum, 0,150-0,250% Bifidobacterium longum, 0,150-0,250% Bifidobacterium infantis, 0,150-0,250% Bacillus clausii, 0,050- 0,150% Lactococcus lactis, 0,050-0,150% Streptococcus thermophilus.

3. The multi-probiotic oil combination according to claim 2 characterized by comprising, in total mixture by weight; 96,296% borage oil, 0,236% patchouli oil, 0,223% mint oil, 0,245% myrrh oil, 0,2% Akkermensia muciniphila, 0,2% Lactobacillus gasseri, 0,2% Bacillus clausii, 0,2% Bacillus subtilis, 0,2% Leuconostoc mesenteroides, 0,2% Lactobacillus rhamnosus, 0,2% Lactobacillus cameliae, 0,2% Lactobacillus acidophilus, 0,2% Lactobacillus bulgaricus, 0,2% Bifidobacterium animalis, 0,2% Bifidobacterium bifidum, 0,2% Bifidobacterium longum, 0,2% Bifidobacterium infantis, 0,2% Bacillus clausii, 0,1% Lactococcus lactis, 0,1% Streptococcus thermophilus .

4. A multi-probiotic oil combination production method developed for osteoporosis regression, regression of menopause/andropause symptoms, prostaglandin/eicosanoid regulation, geriatric support characterized by comprising of the following steps; i. Isolating microorganisms in sterile area at 22°C - 45°C temperature, 35-75% humidity and 2 -12 days interval and purifying isolates, homogeneously mixing with trihalose, maltodextrin, platinose, cyclodextrin, dextrose and all kinds of 6- carbon sugar and all prebiotic fibers in the bioreactor, ii. Freeze drying of microorganisms in a freezer-freezer lyophilizer in a sterile area for 1 - 5 days at a temperature between -80°C and -20°C, iii. Mixing the microorganism combination in the cubic mixer, iv. Adding patchouli, mint and myrrh oils into borage oil, v. Adding the following bacteria to oil and homogenizing the same in the range of 1-5 days, in the sterile area Akkermensia muciniphila, Lactobacillus gasseri,

Bacillus clausii, Bacillus subtilis, Leuconostoc mesenteroides, Lactobacillus rhamnosus, Lactobacillus cameliae, Lactobacillus acidophilus, Lactobacillus bulgaricus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bacillus clausii, Lactococcus lactis, Streptococcus thermophilus, vi. Filling of probiotic and oil combination into amber glass bottles with a peristaltic pump