WO2021215584A1

WO2021215584A1 - Apparatus and method for measuring glycated hemoglobin

Info

Publication number: WO2021215584A1
Application number: PCT/KR2020/008881
Authority: WO
Inventors: 박경원; 문창상; 최윤숙; 전보람
Original assignee: 주식회사 레오바이오
Priority date: 2020-04-20
Filing date: 2020-07-08
Publication date: 2021-10-28
Also published as: KR102174557B1

Abstract

An apparatus for measuring glycated hemoglobin is provided. The apparatus for measuring glycated hemoglobin according to one embodiment of the present invention comprises: a cartridge which accommodates blood and a plurality of drugs therein; a rotation tray part in which the cartridge is disposed and which rotates the cartridge; a driving part which is located on the cartridge and has a nozzle movable in the vertical direction; and a measuring part which is located on the cartridge and measures glycated hemoglobin in the blood, wherein the driving part allows at least one of the blood, the plurality of drugs, or a mixture thereof to be sucked into a nozzle or allows the at least one that is sucked to be discharged to the cartridge, such that the blood is chemically treated through the plurality of drugs.

Description

Apparatus and method for measuring glycated hemoglobin

The present invention relates to an apparatus and method for measuring glycated hemoglobin, and more particularly, to an apparatus and method for automatically treating blood and measuring glycated hemoglobin using a cartridge equipped with both blood and a plurality of drugs, a rotation method, and a nozzle is about

Diabetes mellitus is a group of metabolic diseases in which blood sugar is higher than the normal range for a long period of time. If blood sugar is maintained at a high level, acute complications such as diabetic ketoacidosis and hyperglycemic hyperosmolar non-ketotic coma and long-term complications such as cardiovascular disease, stroke, chronic renal failure, diabetic ulcer, and diabetic retinopathy may occur. Therefore, diabetic patients need to regularly check their blood sugar to prevent various complications, and to maintain the blood sugar level at an appropriate level. Therefore, blood glucose is an important indicator for diagnosing or managing diabetes.

Blood sugar refers to the concentration of glucose in the blood, and the level is not fixed and fluctuates every moment under the influence of factors such as diet and physical activity. Therefore, it is important to understand the average blood sugar, not the instantaneous blood sugar. The most widely used method to determine long-term blood sugar is to measure the glycated hemoglobin (HbA1c) level.

Glycated hemoglobin (HbA1c) refers to a form in which hemoglobin normally present in red blood cells is combined with glucose in blood vessels, that is, blood sugar. When blood sugar is high, the amount of blood sugar that binds to hemoglobin increases, so glycated hemoglobin increases. In addition, since the bound sugar is not used and remains bound to hemoglobin, the average level of glycated hemoglobin is maintained. On the other hand, since the average lifespan of red blood cells is about 3 months, the glycated hemoglobin reflects the average blood sugar level for about 3 months.

SUMMARY OF THE INVENTION An object of the present invention is to provide an apparatus and method for automatically performing a plurality of steps to be passed for measuring glycated hemoglobin.

Another object of the present invention is to provide an apparatus and method using information patterns (eg, barcodes, QR codes) that can minimize errors that occur in the process of measuring glycated hemoglobin.

The problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

An apparatus for measuring glycated hemoglobin according to an embodiment of the present invention for solving the above problems includes: a cartridge accommodating blood and a plurality of drugs therein; a rotating tray unit on which the cartridge is disposed and for rotating the cartridge; a driving unit positioned on the cartridge and having a nozzle movable in the vertical direction; and a measuring unit positioned on the cartridge and measuring the glycated hemoglobin of the blood, wherein the driving unit includes at least one of the blood, the plurality of drugs, or a mixture thereof so that the blood is treated with the plurality of drugs. may be sucked into the nozzle or the sucked at least one may be discharged to the cartridge.

Other specific details of the invention are included in the detailed description and drawings.

According to the present invention as described above, it has various effects as follows.

According to the present invention, since a plurality of steps to be passed for measuring glycated hemoglobin are performed automatically rather than manually, it is possible to measure glycated hemoglobin more conveniently and quickly.

In addition, according to the present invention, since an information pattern capable of minimizing an error occurring in the glycated hemoglobin measurement process is used, it is possible to measure the glycated hemoglobin more accurately.

Effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the following description.

1 is a perspective view schematically illustrating an apparatus for measuring glycated hemoglobin according to an embodiment of the present invention.

2 is a perspective view schematically illustrating an internal configuration of an apparatus for measuring glycated hemoglobin according to an embodiment of the present invention.

3A and 3B are perspective views and conceptual views illustrating a measurement unit according to an embodiment of the present invention.

Figure 4 is a perspective view schematically showing a cartridge according to an embodiment of the present invention.

5 is an exploded perspective view schematically showing a cartridge according to an embodiment of the present invention.

6 is a view schematically showing an upper cartridge according to an embodiment of the present invention.

7 is a view schematically showing a lower cartridge according to an embodiment of the present invention.

8 is a view schematically showing a lower cartridge to which a sealing member according to an embodiment of the present invention is applied.

9, 10A, 10B and 11 are views showing a cartridge and a capillary.

12 is a flowchart illustrating a method for measuring glycated hemoglobin using an apparatus for measuring glycated hemoglobin according to an embodiment of the present invention.

13 to 37 are exemplary views illustrating a method for measuring glycated hemoglobin using the glycated hemoglobin measuring apparatus according to an embodiment of the present invention.

Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described below in detail in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms, and only these embodiments allow the disclosure of the present invention to be complete, and those of ordinary skill in the art to which the present invention pertains. It is provided to fully understand the scope of the present invention to those skilled in the art, and the present invention is only defined by the scope of the claims.

The terminology used herein is for the purpose of describing the embodiments and is not intended to limit the present invention. As used herein, the singular also includes the plural unless specifically stated otherwise in the phrase. As used herein, “comprises” and/or “comprising” does not exclude the presence or addition of one or more other components in addition to the stated components. Like reference numerals refer to like elements throughout, and "and/or" includes each and every combination of one or more of the recited elements. Although "first", "second", etc. are used to describe various elements, these elements are not limited by these terms, of course. These terms are only used to distinguish one component from another. Accordingly, it goes without saying that the first component mentioned below may be the second component within the spirit of the present invention.

Unless otherwise defined, all terms (including technical and scientific terms) used herein will have the meaning commonly understood by those of ordinary skill in the art to which this invention belongs. In addition, terms defined in a commonly used dictionary are not to be interpreted ideally or excessively unless specifically defined explicitly.

Spatially relative terms "below", "beneath", "lower", "above", "upper", etc. It can be used to easily describe the correlation between a component and other components. A spatially relative term should be understood as a term that includes different directions of components during use or operation in addition to the directions shown in the drawings. For example, when a component shown in the drawing is turned over, a component described as “beneath” or “beneath” of another component may be placed “above” of the other component. can Accordingly, the exemplary term “below” may include both directions below and above. Components may also be oriented in other orientations, and thus spatially relative terms may be interpreted according to orientation.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

As used herein, the term "solid reagent" refers to a reagent used to measure glycated hemoglobin, and serves to induce hemolysis. That is, the hemolysis reagent plays a role in inducing hemoglobin to flow out of the blood cells by disintegrating the red blood cells in the blood.

As used herein, the term "decomposition solution" refers to a reagent that serves to wash a test object.

1 is a perspective view schematically illustrating an apparatus for measuring glycated hemoglobin according to an embodiment of the present invention. 2 is a perspective view schematically illustrating an internal configuration of an apparatus for measuring glycated hemoglobin according to an embodiment of the present invention. 3A and 3B are perspective views and conceptual views illustrating a measurement unit according to an embodiment of the present invention.

In one embodiment, the HbA1c measurement apparatus 10 of the present invention may include a light source and a measurement unit (imaging device) therein, and an information pattern (bar code or QR code) in the all-in-one cartridge 100 . ) can be photographed to set error correction conditions that may occur each time the cartridge 100 is manufactured, and when the cartridge 100 is inserted, rotation, elevation, and lowering can be performed by a motor. For example, the cartridge 100 may be a kit that inserts a blood sample and performs chemical processing to measure the glycated hemoglobin, and the glycated hemoglobin measuring apparatus 10 obtains a photographed image while providing light to the chemically treated blood can do. For example, the CMOS camera can perform photographing according to the rotation of the cartridge 100 at a fixed position inside the device 10 , and the user puts the blood-inserted cartridge 100 into the device 10 only, without the need for user manipulation. All operations can be performed automatically.

Specifically, referring to FIGS. 1, 2, 3A and 3B , in an embodiment, the HbA1c measurement apparatus 10 may include a cartridge 100, a main body 200 of the measurement apparatus, and a monitor. The cartridge 100 may contain a plurality of drugs for treating blood corresponding to the measurement target and blood for measuring glycated hemoglobin. The measuring device body 200 may automatically treat the blood contained in the cartridge 200 and measure the glycated hemoglobin.

In an embodiment, the monitor 300 may visually display the progress and result of the glycated hemoglobin measurement through a predefined user interface.

In one embodiment, the cartridge 100 may contain blood and a plurality of drugs therein. For example, the plurality of drugs may include a solid reagent, a decomposition solution, and a reaction solution. The cartridge 100 includes a blood part (R1-1) in which blood is accommodated, a solid reagent part (R1-2) in which a solid reagent is accommodated, a decomposition solution part (R2) in which the decomposition solution is accommodated, and a reaction solution part in which the reaction solution is accommodated. (CL), a membrane unit in which the membrane is accommodated, a washing unit SP, and an information pattern recognition unit (B) may be included. That is, each component included in the cartridge 100 may mean a space capable of accommodating a liquid or solid material.

In an embodiment, the measuring device body 200 may include a driving unit 210 , a measuring unit 220 , and a rotating tray unit 230 . FIG. 2 schematically shows the inside of the measuring device body 200 so that the outer housing is not shown, but the driving unit 210 , the measuring unit 220 and the rotating tray 230 are provided inside the measuring device body 200 . is the composition In addition, the driving unit 210 may be located on the edge and upper portion of the rotating tray unit 230 , and the measuring unit 220 may be located at the edge and above the rotating tray unit 230 .

In one embodiment, the driving unit 210 may be provided with a nozzle 211 positioned on the cartridge 100 and movable in the vertical direction. For example, the driving unit 210 inhales at least one of blood, a plurality of drugs, or a mixture thereof into the nozzle 211 by a preset amount or at least one of the inhaled cartridges so that the blood is treated through a plurality of drugs. (100) can be discharged. That is, the driving unit 210 may transport blood, a plurality of drugs, or a mixture thereof included in the cartridge 100 from one space to another space among a plurality of spaces inside the cartridge 100 . A detailed operation related thereto will be described later with reference to FIGS. 12 to 37 .

In one embodiment, the driving unit 210 is a motor (not shown) that provides a driving force so that the nozzle 211 can move up and down, a toothed portion 214 connected to the motor, and the toothed portion 214 and meshed with the toothed portion 214 ) to include a guide portion 213 capable of up and down movement according to the rotation, and one end is connected to the guide portion 212, the other end is connected to the nozzle 211, and the other end is located on the cartridge 100. can Each of the components of the driving unit 210 may be configured using conventionally known techniques (eg, a piston pump).

For example, the position of the driving unit 210 may be fixed, and accordingly, the nozzle 211 may only move up and down at the fixed position. Here, the fixed position may be a position in which various holes formed in the cartridge 100 to be described later are placed by rotation. That is, the blood or a plurality of drugs of the cartridge 100 by the rotation of the rotation tray 230 may be located at a position in which the nozzle 211 is fixed.

In one embodiment, the rotation tray unit 230 is a cartridge 100 is disposed and can rotate the cartridge (100). For example, the rotating tray unit 230 rotates by an angle required in each step of the glycated hemoglobin measurement process to stop the corresponding configuration of the cartridge 100 at a set position (eg, under the measuring area or nozzle 211). can do. In addition, the rotation tray 230 is designed so as to remain parallel and not deformed during the rotational movement. Meanwhile, the rotating shaft of the rotating tray unit 230 may be rotated by an internal motor or a motor used together with the driving unit 210 .

For example, in the rotary tray unit 230, the blood unit R1-1, the solid reagent unit R1-2, the decomposition solution unit R2, the reaction solution unit CL, and the membrane unit nozzle 211 move up and down. It is possible to rotate the cartridge 100 based on a preset angle to be located in the region (or the nozzle 211 is a fixed position). That is, the cartridge 100 may be provided to surround the internal spaces R1-1, R1-2, R2, CL for accommodating blood or a plurality of drugs in the circumferential direction with respect to the center, and the rotation tray unit 230 ), the inner spaces of the cartridge 100 may be positioned under the nozzle 211 by rotation.

For example, the rotating tray unit 230 may include a rotating tray unit body 231 and a guide unit 233 in which the cartridge 100 can be mounted and a receiving groove 232 capable of being fixed therein is formed. . The rotating tray main body 231 may have a circular tray shape. Here, although not specifically shown in the drawings, the bottom portion on which the cartridge 100 is seated may be rotatably formed on the rotating tray main body 231 . Here, the rotational force may be provided by a separate motor or may be provided by the motor of the driving unit 210 . In addition, the rotating tray main body 231 may move along the guide part 233 in the longitudinal direction of the guide part 233 . That is, as shown in Figure 1, the rotating tray main body 231 can be exposed to the outside by moving outward so that the cartridge 100 can be inserted, and moving inward to nozzle the cartridge 100. It may be located under the 211 and the measuring unit 220 . For each configuration of the rotating tray 230, known techniques may be applied.

In an embodiment, the measuring unit 220 is located on the cartridge 100 and may measure the glycated hemoglobin of blood. The measuring unit 220 is a device that measures the glycated hemoglobin contained in the blood by an optical method. Since hemoglobin (Hb) and glycated hemoglobin (HbA1c) not bound to blood glucose react in different wavelength bands, the measuring unit 220 measures the absorbance of the measurement target material to measure the glycated hemoglobin. In an exemplary embodiment, the glycated hemoglobin measured by the measurement unit 220 is expressed as a numerical value, and the glycated hemoglobin level may be expressed in units of HbA1c%. In this case, the glycated hemoglobin level is the ratio (%) of the glycated hemoglobin (HbA1c) concentration to the hemoglobin (Hb) concentration. The calculation of HbA1c% is based on the National Glycohemoglobin Standardization Program (NGSP), the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), the Japanese Diabetes Spciety (JDS), and the like.

For example, the measurement unit 220 includes a camera 221 for measuring glycated hemoglobin in the drug-treated blood, a red LED 222 emitting red light to the drug-treated blood, and a blue light emitting blue light to the drug-treated blood. The LED 223, the camera 221, the red LED 222, and the blue LED 223 are mounted on the circuit board 224 and the measuring unit 220 includes a connection frame 225 for fixing to be positioned in the measurement area. can do. The camera 221 may be a CMOS CAM. For each configuration of the measurement unit 220, known techniques may be applied.

Meanwhile, as an embodiment, the apparatus for measuring glycated hemoglobin 10 may further include a sensor unit (not shown) or a printer unit (not shown). The sensor unit serves to detect the movement of the driving unit 210 and the rotation tray unit 230 . Through this, the driving unit 210 and the rotation tray unit 230 rotate by a set angle, and can be accurately stopped at a predetermined position. The printer unit serves to output the glycated hemoglobin measurement result on paper or the like.

Figure 4 is a perspective view schematically showing a cartridge according to an embodiment of the present invention. 5 is an exploded perspective view schematically showing a cartridge according to an embodiment of the present invention. 6 is a view schematically showing an upper cartridge according to an embodiment of the present invention. 7 is a view schematically showing a lower cartridge according to an embodiment of the present invention. 8 is a view schematically showing a lower cartridge to which a sealing member according to an embodiment of the present invention is applied. 9, 10A, 10B and 11 are views showing a cartridge and a capillary.

4 to 11 , the cartridge 100 may contain blood and a plurality of drugs therein. For example, the plurality of drugs may include a solid reagent, a decomposition solution, and a reaction solution. For example, the cartridge 100 may be a circular kit.

In one embodiment, the cartridge 100 may be provided with a plurality of spaces therein, and blood, a plurality of drugs or cleaning materials may be provided in the plurality of spaces, respectively. In addition, the cartridge 100 exposes the plurality of spaces to the outside so that the nozzle 211 of the driving unit 210 can suck or discharge blood provided in the plurality of spaces, and the nozzle 211 can penetrate. A plurality of holes may be provided. Here, the plurality of spaces are named as a blood part R1-1, a washing part SP, a solid reagent part R1-2, a decomposition solution part R2, a reaction solution part CL, and a membrane part, and will be described below. On the other hand, the information pattern recognition unit (B) may be a groove formed on the upper surface of the cartridge (100).

For example, the blood part R1-1, the washing part SP, the solid reagent part R1-2, the decomposition solution part R2, the reaction solution part CL, and the membrane part are the center of the circular cartridge 100. It may be formed along the circumferential direction based on the .

For example, the blood unit R1-1 may contain blood, the solid reagent unit R1-2 may contain a solid reagent, and the decomposition solution unit R2 may contain a decomposition solution, , the reaction solution unit CL may receive the reaction solution, the membrane unit may accommodate the membrane, and the washing unit SP may include a material for washing the nozzle 211 (hereinafter referred to as a cleaning material), The information pattern recognition unit B may accommodate the information pattern sticker 150 . The solid reagent, the decomposition solution, the reaction solution, and the washing material may each have a capacity that can be repeatedly supplied several times or more in advance. For example, a plurality of drugs may be pre-stored before the cartridge 100 is actually manufactured and distributed.

For example, the reaction solution may be a diluent for controlling the concentration of blood. The decomposition solution is a kind of washer solution, and it is a reagent that provides a washing solution so that the material to be measured is washed and in a state that is easy to measure. The decomposition solution may include effects other than washing, if necessary, and is not limited only to the washing effect. A solid reagent is a reagent used to measure glycated hemoglobin, and is a reagent that induces hemolysis. That is, the solid reagent plays a role in inducing hemoglobin to flow out of the blood cells by disintegrating the red blood cells in the blood to measure the glycated hemoglobin in the collected blood. The solid reagent may include effects other than inducing hemolysis, if necessary, and is not limited only to the hemolytic effect.

In one embodiment, the cartridge 100 is an upper cartridge 110, a lower cartridge 120, a capillary 130, a point sticker 140, an information pattern sticker 150, a sealing member 160, a membrane ( 170 ) and a container 180 .

In one embodiment, the upper cartridge 110 may be combined with the lower cartridge 120 to define the outer shape of the cartridge 100 and protect the internal components. For example, the upper cartridge 110 has a blood part hole 113 for exposing blood to the outside so that the nozzle 211 can pass through, a solid reagent part hole 117 for exposing a solid reagent to the outside, and a decomposition solution to the outside. The decomposition solution part hole 118 exposed to the outside, the reaction solution part hole 119 exposing the reaction solution to the outside, the membrane part hole 116 exposing the membrane 170 to the outside, the washing part exposing the cleaning material to the outside A hole 114 may be provided.

In one embodiment, the upper cartridge 110 has a point sticker unit 111 on which the point sticker 140 is seated, a capillary hole 112 into which the capillary 130 is inserted, and a capillary 130 . A capillary groove 131 to be seated and an information pattern sticker unit 115 in which the information pattern sticker 150 is seated may be additionally provided. Here, the point sticker unit 111 , the capillary groove 131 , and the information pattern sticker unit 115 may be grooves.

In one embodiment, the lower cartridge 120 includes a blood container container 123 for accommodating blood, a solid reagent container 127 for accommodating a solid reagent, a decomposition solution container 128 for accommodating a decomposition solution, and a reaction solution. The reaction solution container 129 for accommodating the membrane 170, the membrane container 126 for accommodating the membrane 170, the cleaning part container 124 for accommodating the cleaning material, the barcode area 125, and the capillary area 132 are provided. can be provided

In one embodiment, the point sticker 140 may indicate the direction in which the cartridge 100 is inserted into the rotation tray unit 230 and may be disposed on the point sticker unit 111 .

In an embodiment, the information pattern sticker 150 may include information related to a plurality of drugs and may be disposed in the information pattern recognition unit B.

In one embodiment, the sealing member 160 may cover the solid reagent container 127, the decomposition solution container 128, and the reaction solution container 129 to prevent leakage of the solid reagent, the decomposition solution, and the reaction solution. . For example, the sealing member 160 may be pierced when the nozzle 211 descends to suck the solid reagent, decomposition solution, or reaction solution. That is, the sealing member 160 may have a sealing function for the circulation of the cartridge 100 , and may be configured to open a space that is sealed by being pierced by the nozzle 211 during actual measurement.

In one embodiment, the membrane 170 has a configuration in which a mixed solution in which blood and a plurality of drugs are mixed is seated and a material to be measured is substantially located. For example, the membrane 170 is made of a material with selective permeability. As a specific example, the membrane 170 may be a filter material capable of not only separating particles by selectively passing specific components, but also separating dissolved substances dissolved in a liquid solution.

In another embodiment, the membrane 170 is configured to be replaced every time the glycated hemoglobin measurement is performed. As a specific example, since the cartridge 100 is configured in a form capable of vertical separation, the membrane 170 can be replaced by removing the upper cartridge 110 . Accordingly, the membrane 170 may be conveniently mounted or removed, and may be disposed at an accurate position. Meanwhile, the membrane 170 is maintained in a flat state without bending so that the solution can be evenly distributed.

In one embodiment, the container 180 may be inserted into the solid reagent unit container 127 to accommodate the solid reagent.

In one embodiment, the capillary 130 is first manufactured and is inserted into the cartridge 100 when the cartridge 100 is shipped, so that the user can pull it out and use it after it is shipped together. The capillary 130 stores the blood filled by the user and may be seated in the capillary groove 131 formed in the upper cartridge 110, the capillary insertion part 131 and the capillary container ( 132) may be included. For example, the capillary insertion part 131 may be inserted into the capillary insertion hole 112 provided in the upper cartridge 110 and communicating with the blood part container 123 , and the capillary container 132 . ) is connected to the capillary insert so as to be detachable by an external force and can accommodate pre-stored blood.

For example, the cartridge 100 may be distributed with a plurality of drugs therein, and the capillary 130 in a state in which no blood is provided therein is included. Thereafter, when actually measuring the glycated hemoglobin, the capillary 130 may be combined with the cartridge 100 again as described below after the blood, which is the measurement target material, is collected while separated from the cartridge 100 . .

For example, the capillary container 132 may be manufactured so that a specific volume (eg, 5ul) required for measurement of glycated hemoglobin is accurately collected.

For example, the capillary 130 filled with blood may be inserted into the capillary insertion hole 112 of the cartridge 100 as shown in FIG. 9, and after being inserted as shown in FIG. 10A, the capillary as shown in FIG. The blood filled in the filler container 132 may be discharged to the blood part R1-1 of the cartridge 100 through the capillary insertion part 131 . Thereafter, the capillary container 132 may be separated from the capillary insertion unit 131 by the external force of the person performing the test, and only the capillary insertion unit 131 may be left in the cartridge 100 . . Through this, the capillary container 132 may not act as an element that prevents rotation of the cartridge 100 .

12 is a flowchart illustrating a method for measuring glycated hemoglobin using an apparatus for measuring glycated hemoglobin according to an embodiment of the present invention. 13 to 37 are exemplary views illustrating a method for measuring glycated hemoglobin using the glycated hemoglobin measuring apparatus according to an embodiment of the present invention. The operations of FIG. 12 may be performed by the respective components of the apparatus 100 for measuring glycated hemoglobin shown in FIG. 2 .

On the other hand, the arrows respectively indicated in the drawings mean the moving direction of the nozzle 211 and the rotation direction of the cartridge 100 for proceeding from the current drawing to the next drawing. That is, for example, when a downward arrow and a clockwise arrow are indicated in the current drawing, the direction of the next operation (next drawing) of the nozzle 211 is the downward direction, and the next operation of the cartridge 100 (next drawing) ) may mean a clockwise direction.

2 to 37 , in an embodiment, in operation S1, blood may be injected into the blood unit R1-1 using the capillary 130 as shown in FIGS. 9 to 11 . have. Operation S1 may be performed manually or may be automatically performed through a known configuration such as a robot arm. The cartridge 100 to be described below includes a blood part (R1-1), a washing part (SP), a solid reagent part (R1-2), a decomposition solution part (R2), a reaction solution part (CL) and a membrane part (M). It is assumed that blood, washing material, solid reagent, decomposition solution, reaction solution, and membrane are provided in each.

On the other hand, in the present embodiment, the driving unit 210 may provide a desired amount by sucking a larger amount of the target material into the nozzle 211 than the amount to be provided and then discarding the remaining amount after providing the predetermined amount. That is, this is to prevent an error from occurring due to the remaining amount of the nozzle 211 . Therefore, washing with the reaction solution of the reaction solution unit CL can be performed so that the next drug inhalation is not affected.

In one embodiment, in operation S2 , the measurement unit 220 may recognize the information pattern sticker 150 placed on the information pattern recognition unit B of the cartridge 100 . Here, the information pattern sticker 150 may include a barcode or QR code. For example, the cartridge 100 may be disposed as shown in FIG. 14 in the empty space of the rotating tray body 231 shown in FIG. 13 . This may be a case in which the cartridge 100 is inserted into the rotation tray body 231 according to the direction of the point sticker 140 (from right to left on the basis of FIG. 14 ). For example, when the measurement unit 220 is located in the 7 o'clock direction (all time directions to be described below are based on the center of the cartridge 100) with respect to the center of the cartridge 100 of FIG. 14, the rotation tray unit 230 ) may rotate the cartridge 100 by a preset angle in the counterclockwise direction as shown in FIG. 14 . Accordingly, as shown in FIG. 15 , the barcode unit B is positioned at 7 o'clock, and the measurement unit 220 can recognize the information pattern sticker 150 located in the barcode unit B. As shown in FIG. For example, an algorithm may be preset so that the driving unit 210 obtains information on a plurality of drugs included in the information pattern to allow error correction.

In an embodiment, in operation S3 , the driving unit 210 may suck the reaction liquid from the reaction liquid unit CL using the nozzle 211 . First, in this embodiment, the nozzle 211 will be described as being disposed at a fixed position in the 12 o'clock direction. The rotating tray unit 230 rotates the cartridge 100 in a counterclockwise direction as shown in FIG. 15 to position the reaction liquid unit CL under the nozzle 211 as shown in FIG. 16 and the driving unit ( As shown in FIG. 15 , the 210 may lower the nozzle 211 to the reaction liquid part CL to suck the reaction liquid into the nozzle 211 as illustrated in FIG. 16 . For example, 150ul of the reaction solution may be sucked into the nozzle 211 .

In an embodiment, in operation S4 , after the blood and the reaction solution are mixed in the blood unit R1-1, the driving unit 210 may suck the first mixed solution from the blood unit R1-1 through the nozzle 211. can For example, the driving unit 210 may raise the nozzle 211 shown in FIG. 16 as shown in FIG. 17 , and the rotating tray 230 rotates the cartridge 100 in a counterclockwise direction as shown in FIG. 16 . may be rotated to position the blood unit R1-1 under the nozzle 211 as shown in FIG. 17, and the driving unit 210 may move the nozzle 211 to the blood unit R1- as shown in FIG. 1) and the suctioned reaction solution can be discharged to the blood unit R1-1, and the driving unit 210 lowers the nozzle 211 to the blood unit R1-1 as shown in FIG. The first mixed solution in which the discharged reaction solution and blood are mixed may be sucked from the blood part R1-1 to the nozzle 211 . That is, for example, 150 ul of the reaction solution is discharged to the blood unit R1-1, and after the blood and the reaction solution are mixed, 150 ul of the first mixed solution may be sucked into the nozzle 211 .

In one embodiment, in operation S5, after the first mixed solution and the solid reagent are mixed in the solid reagent unit R1-2, the driving unit 210 uses the nozzle 211 to remove the liquid from the solid reagent unit R1-2. 2 The mixture can be inhaled. For example, the driving unit 210 may raise the nozzle 211 as shown in FIGS. 18 and 19, and the rotating tray 230 rotates the cartridge 100 in a clockwise direction as shown in FIG. As shown, the solid reagent unit R1-2 may be positioned under the nozzle 211, and the driving unit 210 moves the nozzle 211 to the solid reagent unit R1-2 as shown in FIGS. 19 and 20. ) and the suctioned first mixed solution can be discharged to the solid reagent unit R1-2, and the driving unit 210 moves the nozzle 211 to the solid reagent unit R1-2 as shown in FIG. The second mixed solution in which the first mixed pressure solution and the solid reagent are mixed by descending may be sucked from the solid reagent unit R1-2 to the nozzle 211. For example, after discharging the entire amount of the first mixed solution to the solid reagent unit R1-2, mixing the first mixed solution and the solid reagent, 100ul of the second mixed solution may be sucked into the nozzle 211.

In an embodiment, in operation S6 , the driving unit 210 may discharge the second mixed solution to the membrane unit M using the nozzle 211 . For example, the driving unit 210 raises the nozzle 211 again as shown in FIG. 20, and the rotary tray 230 rotates the cartridge 100 in a clockwise direction as shown in FIG. 21 , the membrane part M may be positioned below the nozzle 211 , and the driving part 210 lowers the nozzle 211 to the membrane part M as shown in FIGS. 21 and 22 . A portion of the second mixed solution sucked through the evaporator may be discharged to the membrane part (M). For example, 25ul of the second mixed solution may be discharged to the membrane part (M).

In one embodiment, in operation S7, the driving unit 210 may use the nozzle 211 to discharge the remaining amount of the second mixed solution to the solid reagent unit R1-2. For example, the driving unit 210 may raise the nozzle 211 as shown in FIG. 22 , and the rotating tray 230 rotates the cartridge 100 in a counterclockwise direction as shown in FIG. 22 . As shown in FIG. 23, the solid reagent unit R1-2 can be positioned under the nozzle 211, and the driving unit 210 moves the nozzle 211 to the solid reagent as shown in FIGS. 23 and 24. The remainder of the second mixed solution sucked by descending to the part R1-2 may be discharged to the solid reagent part R1-2. For example, the remaining amount of the second mixed solution 25ul may be discharged to the solid reagent unit R1-2.

In an embodiment, in operation S8 , the driving unit 210 may wash the nozzle 211 using the reaction liquid in the reaction liquid unit CL. For example, the driving unit 210 may raise the nozzle 211 as shown in FIG. 24 , and the rotating tray unit 230 rotates the cartridge 100 in a counterclockwise direction as shown in FIG. The reaction liquid part CL can be positioned under the nozzle 211 as shown in FIG. 25 and the driving part 210 lowers the nozzle 211 to the reaction liquid part CL as shown in FIGS. It can be washed with liquid.

In an embodiment, in operation S9 , the driving unit 210 may bubble-clean the nozzle 211 in the washing unit SP. For example, as shown in FIG. 26 , the driving unit 210 may raise the nozzle 211 , and the rotating tray 230 rotates the cartridge 100 clockwise as shown in FIG. 26 to rotate the nozzle 211 as shown in FIG. ) It is possible to position the washing unit (SP) provided in the cartridge 100 down, and the driving unit 210 lowers the nozzle 211 to the washing unit SP as shown in FIGS. 27 and 28 to the nozzle 211. may be washed with a cleaning material (eg, cleaning cotton) included in the cleaning unit SP.

In an embodiment, in operation S10 , the driving unit 210 may suck the decomposition solution from the decomposition solution unit R2 using the nozzle 211 . For example, the driving unit 210 may raise the nozzle 211, and the rotating tray unit 230 rotates the cartridge 100 in a counterclockwise direction as shown in FIG. 28 under the nozzle 211 as shown in FIG. As shown in FIGS. 29 and 30, the driving unit 210 lowers the nozzle 211 to the decomposition solution unit R2 to suck the decomposition solution into the nozzle 211. have. For example, 100ul of the decomposition solution may be sucked into the nozzle 211 .

In one embodiment, in operation S11 , the driving unit 210 may discharge the decomposition solution to the membrane unit M using the nozzle 211 . For example, the driving unit 210 may raise the nozzle 211 as shown in FIGS. 30 and 31 , and the rotating tray 230 rotates the cartridge 100 clockwise as shown in FIG. Similarly, the membrane part M can be positioned under the nozzle 211, and the driving part 210 lowers the nozzle 211 to the membrane part M as shown in FIGS. 31 and 32 to remove some of the suctioned decomposition solution. It can be discharged to the membrane part (M). For example, 25ul of the decomposition solution may be discharged to the membrane part (M).

In one embodiment, in operation S12 , the driving unit 210 may discharge the remaining amount of the decomposition solution to the decomposition solution unit R2 using the nozzle 211 . For example, the driving unit 210 may raise the nozzle 211 as shown in FIGS. 32 and 33 , and the rotating tray 230 rotates the cartridge 100 in a counterclockwise direction as shown in FIG. The decomposition solution unit R2 can be positioned under the nozzle 211 as shown, and the driving unit 210 lowers the nozzle 211 to the decomposition solution unit R2 as shown in FIGS. 33 and 34 and sucks the decomposition solution. The remainder may be discharged to the decomposition solution part (R2). For example, the remaining amount of the decomposition solution 75ul may be discharged to the decomposition solution part (R2).

In an embodiment, in operation S13 , the driving unit 210 may bubble-clean the nozzle 211 in the washing unit SP. For example, the driving unit 210 may raise the nozzle 211 as shown in FIGS. 34 and 35 , and the rotating tray 230 rotates the cartridge 100 in a clockwise direction as shown in FIG. Similarly, the washing unit SP provided in the cartridge 100 can be positioned below the nozzle 211, and the driving unit 210 lowers the nozzle 211 to the washing unit SP as shown in FIGS. 35 and 36 . The nozzle 211 may be cleaned with a cleaning material (eg, cleaning cotton) included in the cleaning unit SP.

In an embodiment, in operation S14 , the measurement unit 220 may measure a result value in the membrane unit M. For example, the driving unit 210 may raise the nozzle 211 as shown in FIGS. 36 and 37 , and the rotating tray 230 rotates the cartridge 100 clockwise as shown in FIGS. 36 and 37 . The measuring unit 220 may position the membrane unit M in the measurable area (7 o'clock), and the measuring unit 220 includes a third mixed solution in which the second mixed solution and the decomposition solution contained in the membrane M) are mixed. The result can be obtained by measuring the glycated hemoglobin in

In one embodiment, in operation S15 , the driving unit 210 may eject the cartridge 100 . For example, the rotating tray unit 230 may expose the rotating tray unit body 231 to the outside as shown in FIG. 1 along the guide unit 233, and thus the cartridge 100 exposed to the outside can be taken out. have.

As described above, when the cartridge 100 filled with blood and a plurality of drugs is inserted into the glycated hemoglobin device 10 , the blood is automatically treated with drugs and the glycated hemoglobin can be measured.

An apparatus for measuring glycated hemoglobin according to an embodiment of the present invention includes: a cartridge accommodating blood and a plurality of drugs therein; a rotating tray unit on which the cartridge is disposed and for rotating the cartridge; a driving unit positioned on the cartridge and having a nozzle movable in the vertical direction; and a measuring unit positioned on the cartridge and measuring the glycated hemoglobin of the blood, wherein the driving unit includes at least one of the blood, the plurality of drugs, or a mixture thereof so that the blood is treated with the plurality of drugs. may be sucked into the nozzle or the sucked at least one may be discharged to the cartridge.

According to various embodiments, the plurality of drugs includes a solid reagent, a decomposition solution, and a reaction solution, and the cartridge includes a blood unit in which the blood is accommodated, a solid reagent unit in which the solid reagent is accommodated, and the decomposition solution is accommodated in the cartridge. It may include a decomposition solution part, a reaction solution part in which the reaction solution is accommodated, and a membrane part in which the membrane is accommodated.

According to various embodiments, the rotary tray unit rotates the cartridge based on a preset angle such that the blood unit, the solid reagent unit, the decomposition solution unit, the reaction solution unit, or the membrane unit is located in the area in which the nozzle moves up and down. can be rotated

According to various embodiments, the cartridge includes a blood part hole for exposing the blood to the outside so that the nozzle can pass through, a solid reagent part hole for exposing the solid reagent to the outside, and a decomposition solution for exposing the decomposition solution to the outside. an upper cartridge having a secondary hole, a reaction liquid part hole exposing the reaction solution to the outside, and a membrane part hole exposing the membrane to the outside; and a blood container for accommodating the blood, a solid reagent container for accommodating the solid reagent, a decomposition solution container for accommodating the decomposition solution, a reaction solution container for accommodating the reaction solution, and a membrane container for accommodating the membrane. It may include; a lower cartridge having a.

According to various embodiments, the cartridge may include: a sealing member covering the solid reagent container, the decomposition solution container, and the reaction solution container to prevent leakage of the solid reagent, the decomposition solution, and the reaction solution; and a container inserted into the solid reagent unit container to accommodate the solid reagent.

According to various embodiments, the cartridge further includes a capillary in which the blood is stored in advance and seated in a groove formed in the upper cartridge, the capillary being provided in the upper cartridge and communicating with the blood vessel container. a capillary insertion unit capable of being inserted into the capillary insertion hole; and a capillary container connected to the capillary insertion part to be detachable by an external force and accommodating the pre-stored blood.

According to various embodiments, the cartridge may include a cleaning unit provided with a material for cleaning the nozzle; an information pattern recognition unit on which information pattern stickers including information related to the plurality of drugs are disposed; and a point sticker unit in which a point sticker indicating a direction in which the cartridge is inserted into the rotation tray unit is disposed.

A method for measuring glycated hemoglobin using the apparatus for measuring glycated hemoglobin according to claim 2 according to an embodiment of the present invention comprises: positioning the reaction solution part under the nozzle by rotating the cartridge by the rotating tray part; sucking the reaction liquid into the nozzle by the driving unit lowering the nozzle to the reaction liquid part; rotating the cartridge by the rotating tray unit to position the blood unit under the nozzle; discharging the sucked reaction solution to the blood unit by lowering the nozzle to the blood unit by the driving unit; the driving unit lowering the nozzle to the blood unit and sucking the discharged reaction solution and the first mixed solution in which the blood is mixed from the blood unit to the nozzle; positioning the solid reagent unit under the nozzle by rotating the rotating tray unit to the cartridge; discharging the suctioned first mixed solution to the solid reagent unit by lowering the nozzle to the solid reagent unit by the driving unit; the driving unit lowering the nozzle to the solid reagent unit and sucking the discharged first mixed pressure solution and the second mixed solution mixed with the solid reagent from the solid reagent unit to the nozzle; rotating the cartridge by the rotating tray unit to position the membrane unit under the nozzle; and discharging a portion of the suctioned second mixed solution to the membrane unit by the driving unit lowering the nozzle to the membrane unit.

According to various embodiments, the rotating tray rotating the cartridge to position the solid reagent unit under the nozzle; discharging the remainder of the suctioned second mixed solution to the solid reagent unit by lowering the nozzle to the solid reagent unit by the driving unit; rotating the cartridge by the rotating tray unit to position the reaction liquid unit under the nozzle; washing the nozzle with the reaction solution by lowering the nozzle to the reaction solution unit by the driving unit; rotating the cartridge by the rotating tray unit to position the washing unit provided in the cartridge under the nozzle; and lowering the nozzle to the washing unit by the driving unit to wash the nozzle with a washing material included in the washing unit.

According to various embodiments, the rotating tray rotating the cartridge to position the decomposition solution unit under the nozzle; sucking the decomposition solution into the nozzle by the driving unit lowering the nozzle to the decomposition solution unit; rotating the cartridge by the rotating tray unit to position the membrane unit under the nozzle; discharging a portion of the suctioned decomposition solution to the membrane unit by the driving unit lowering the nozzle to the membrane unit; Positioning the decomposition solution unit under the nozzle by rotating the rotating tray unit the cartridge; discharging the rest of the suctioned decomposition solution to the decomposition solution unit by lowering the nozzle to the decomposition solution unit by the driving unit; rotating the cartridge by the rotating tray unit to position the membrane unit in an area where the measuring unit can measure; and measuring, by the measuring unit, the glycated hemoglobin in the third mixed solution in which the second mixed solution and the decomposition solution accommodated in the membrane unit are mixed.

As mentioned above, although embodiments of the present invention have been described with reference to the accompanying drawings, those skilled in the art to which the present invention pertains can realize that the present invention can be embodied in other specific forms without changing its technical spirit or essential features. you will be able to understand Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims

a cartridge containing blood and a plurality of drugs therein;

a rotating tray unit on which the cartridge is disposed and for rotating the cartridge;

a driving unit positioned on the cartridge and having a nozzle movable in the vertical direction; and

a measuring unit positioned on the cartridge and measuring the glycated hemoglobin of the blood;

The driving unit sucks at least one of the blood, the plurality of drugs, or a mixture thereof to the nozzle or discharges the sucked at least one to the cartridge so that the blood is treated with the plurality of drugs A glycated hemoglobin measuring device.
According to claim 1,

The plurality of drugs include a solid reagent, a decomposition solution and a reaction solution,

The cartridge may include a blood unit accommodating the blood, a solid reagent unit accommodating the solid reagent, a decomposition solution unit accommodating the decomposition solution, a reaction solution unit accommodating the reaction solution, and a membrane unit accommodating the membrane A glycated hemoglobin measuring device.
3. The method of claim 2,

The rotation tray part rotates the cartridge based on a preset angle so that the blood part, the solid reagent part, the decomposition solution part, the reaction solution part, or the membrane part are located in the area where the nozzle moves up and down. A glycosylated hemoglobin measurement device.
3. The method of claim 2,

The cartridge is

A blood part hole for exposing the blood to the outside so that the nozzle can pass through, a solid reagent part hole for exposing the solid reagent to the outside, a degradation solution part hole for exposing the decomposition solution to the outside, and exposing the reaction solution to the outside an upper cartridge having a hole for the reaction solution and a hole for the membrane for exposing the membrane to the outside; and

A blood vessel container accommodating the blood, a solid reagent container accommodating the solid reagent, a decomposition solution container accommodating the decomposition solution, a reaction solution container accommodating the reaction solution, and a membrane container accommodating the membrane. A glycated hemoglobin measuring apparatus comprising a lower cartridge.
5. The method of claim 4,

The cartridge is

a sealing member covering the solid reagent container, the decomposition solution container, and the reaction solution container to prevent leakage of the solid reagent, the decomposition solution, and the reaction solution; and

The apparatus for measuring glycated hemoglobin further comprising; a container inserted into the solid reagent container to accommodate the solid reagent.
6. The method of claim 5,

The cartridge further includes a capillary in which the blood is pre-stored and seated in a groove formed in the upper cartridge,

The capillary is

a capillary insertion unit provided in the upper cartridge and insertable into a capillary insertion hole communicating with the blood vessel container; and

and a capillary container connected to the capillary insertion part to be detachable by an external force and accommodating the pre-stored blood.
5. The method of claim 4,

The cartridge is

a cleaning unit provided with a material for cleaning the nozzle;

an information pattern recognition unit on which information pattern stickers including information related to the plurality of drugs are disposed; and

The apparatus for measuring glycated hemoglobin according to claim 1, further comprising: a point sticker in which a point sticker indicating a direction in which the cartridge is inserted into the rotating tray is disposed.
A cartridge for measuring glycated hemoglobin, the cartridge comprising:

Having internal spaces for accommodating blood and a plurality of drugs therein, respectively,

The cartridge for measuring glycated hemoglobin, wherein the inner spaces are provided to surround the center of the cartridge in a circumferential direction.
9. The method of claim 8,

The plurality of drugs include a solid reagent, a decomposition solution and a reaction solution,

The internal spaces include a blood part accommodating the blood, a solid reagent part accommodating the solid reagent, a decomposition solution part accommodating the decomposition solution, a reaction solution part accommodating the reaction solution, and a membrane part accommodating the membrane A cartridge for measuring glycated hemoglobin.
10. The method of claim 9,

A cartridge for measuring glycated hemoglobin, characterized in that it further comprises an upper cartridge and a lower cartridge that define the outer shape of the cartridge and protect the internal components.
11. The method of claim 10,

The upper cartridge includes a blood part hole for exposing the blood to the outside, a solid reagent part hole for exposing the solid reagent to the outside, a degradation solution part hole for exposing the decomposition solution to the outside, and a reaction for exposing the reaction solution to the outside. a liquid part hole and a membrane part hole exposing the membrane to the outside;

The lower cartridge includes a blood container for accommodating the blood, a solid reagent container for accommodating the solid reagent, a decomposition solution container for accommodating the decomposition solution, a reaction solution container for accommodating the reaction solution and the membrane. A cartridge for measuring glycated hemoglobin, characterized in that it comprises a membrane container.
12. The method of claim 11,

a sealing member covering the solid reagent container, the decomposition solution container, and the reaction solution container to prevent leakage of the solid reagent, the decomposition solution, and the reaction solution; and

The cartridge for measuring glycated hemoglobin further comprising; a container inserted into the solid reagent container to accommodate the solid reagent.
13. The method of claim 12,

The cartridge for measuring glycated hemoglobin, wherein the blood is pre-stored and further comprising a capillary seated in a groove formed in the upper cartridge.
14. The method of claim 13,

The capillary is provided in the upper cartridge,

a capillary insertion unit insertable into a capillary insertion hole communicating with the blood vessel container; and

and a capillary container connected to the capillary insertion part to be detachable by an external force and accommodating the pre-stored blood.
12. The method of claim 11,

a cleaning unit provided with a material for cleaning the nozzle;

an information pattern recognition unit on which information pattern stickers including information related to the plurality of drugs are disposed; and

A cartridge for measuring glycated hemoglobin further comprising a point sticker on which a point sticker indicating a direction in which the cartridge is inserted into the rotating tray is disposed.
12. The method of claim 11, wherein the upper cartridge,

For measuring glycated hemoglobin, characterized in that it further comprises a point sticker unit in which the point sticker is seated, a capillary hole in which the capillary is inserted, a capillary groove in which the capillary is seated, and an information pattern sticker unit in which the information pattern sticker is seated. cartridge.
10. The method of claim 9,

The membrane is a cartridge for measuring glycated hemoglobin, characterized in that the mixed solution of the blood and the plurality of drugs is seated.
Positioning the reaction liquid part under the nozzle by rotating the rotating tray unit cartridge;

sucking the reaction liquid into the nozzle by a driving unit lowering the nozzle to the reaction liquid part;

rotating the cartridge by the rotating tray unit to position the blood unit under the nozzle;

discharging the sucked reaction solution to the blood unit by lowering the nozzle to the blood unit by the driving unit;

the driving unit lowering the nozzle to the blood unit and sucking the discharged reaction solution and the first mixed solution in which the blood is mixed from the blood unit to the nozzle;

positioning the solid reagent unit under the nozzle by rotating the rotating tray unit to the cartridge;

discharging the suctioned first mixed solution to the solid reagent unit by lowering the nozzle to the solid reagent unit by the driving unit;

the driving unit lowering the nozzle to the solid reagent unit and sucking the discharged second mixed solution in which the first mixed pressure solution and the solid reagent are mixed from the solid reagent unit to the nozzle;

rotating the cartridge by the rotating tray unit to position the membrane unit under the nozzle; and

and discharging a portion of the suctioned second mixed solution by the driving unit lowering the nozzle to the membrane unit to the membrane unit.
19. The method of claim 18,

positioning the solid reagent unit under the nozzle by rotating the rotating tray unit to the cartridge;

discharging the remainder of the suctioned second mixed solution to the solid reagent unit by lowering the nozzle to the solid reagent unit by the driving unit;

rotating the cartridge by the rotating tray unit to position the reaction liquid unit under the nozzle;

washing the nozzle with the reaction solution by lowering the nozzle to the reaction solution unit by the driving unit;

rotating the cartridge by the rotating tray unit to position the washing unit provided in the cartridge under the nozzle; and

and the driving unit lowers the nozzle to the washing unit to wash the nozzle with a washing material included in the washing unit.
19. The method of claim 18,

Positioning the decomposition solution unit under the nozzle by rotating the rotating tray unit the cartridge;

sucking the decomposition solution into the nozzle by the driving unit lowering the nozzle to the decomposition solution unit;

rotating the cartridge by the rotating tray unit to position the membrane unit under the nozzle;

discharging a portion of the suctioned decomposition solution to the membrane unit by the driving unit lowering the nozzle to the membrane unit;

Positioning the decomposition solution unit under the nozzle by rotating the rotating tray unit the cartridge;

discharging the rest of the suctioned decomposition solution to the decomposition solution unit by lowering the nozzle to the decomposition solution unit by the driving unit;

rotating the cartridge by the rotating tray unit to position the membrane unit in an area where the measuring unit can measure; and

and measuring, by the measuring unit, the glycated hemoglobin in the third mixed solution in which the second mixed solution accommodated in the membrane unit and the decomposition solution are mixed.