Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/47—Quinolines; Isoquinolines
  • A61K31/472—Non-condensed isoquinolines, e.g. papaverine
  • A61K31/4725—Non-condensed isoquinolines, e.g. papaverine containing further heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P11/00—Drugs for disorders of the respiratory system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P11/00—Drugs for disorders of the respiratory system
  • A61P11/06—Antiasthmatics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P13/00—Drugs for disorders of the urinary system
  • A61P13/12—Drugs for disorders of the urinary system of the kidneys
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P3/00—Drugs for disorders of the metabolism
  • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
  • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P9/00—Drugs for disorders of the cardiovascular system
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
  • C07D401/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
  • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
  • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
  • C07D471/04—Ortho-condensed systems

Definitions

• the present invention relates to tetrahydroisoquinoline compounds. More specifically, the present invention relates to tetrahydroisoquinoline compounds that are Nrf2 activators. The present invention also relates to processes for the preparation of these compounds, to pharmaceutical compositions comprising them, and to their use in the treatment of diseases or disorders associated with Nrf2 activation and/or inhibition of Keapl -Nrf2 protein-protein interactions.
• Nuclear factor erythroid 2-related factor 2 (Nrf2) is a basic leucine zipper (bZIP) transcription factor and a member of the Cap ‘n’ Collar (CNC) family of transcription factors. It is a key master of the inducible cell defence system, mediating the expression of more than 100 oxidative stress-related genes that include phase I and II detoxification enzymes and antioxidant proteins. These genes all contain the antioxidant response element (ARE) in their promoter regulatory regions, which is the binding target of Nrf2.
• ARE antioxidant response element
• Nrf2 the adaptor protein
• Keapl a cytosolic actin-bound repressor protein, which binds to Nrf2 and leads to proteasomal degradation via the Cul3-based E3 ubiquitin ligase complex.
• Keapl is inactivated leading to an increase in the level of de novo synthesised Nrf2 which translocates to the nucleus, binds to AREs with a resulting up-regulation in cytoprotective gene expression.
• Nrf2 mRNA expression in CORD subjects was significantly lower than that in control subjects and Nrf2 mRNA were negatively correlated with pack year.
• Nrf2 protein in CORD subjects was significantly lower than that in control subjects.
• CSE-induced A549 cell apoptosis was increased in a time-dependent and concentration- dependent manner, and was significantly increased by Nrf2 knockdown (Yamada, BMC Pulmonary Medicine, doi: 10.1186/s12890-016-0189-1 ). Therefore, elevation of Nrf2 levels in the lungs of CORD patients should lead to a reduction in the inflammatory processes that lead to deleterious structural modifications of the lung and slow disease progression.
• Nrf2 may also be expected to show positive benefits in other respiratory diseases that exhibit oxidative stress components (Cho, Toxicol Appl Pharmacol, doi: 10.1016/j.taap.2009.07.024) such as acute, chronic and severe asthma (Sussan, Am J Physiol Lung Cell Mol Physiol, doi:10.1152/ajplung.00398.2014), acute lung injury/acute respiratory distress syndrome, with or without accompanying multi organ dysfunction syndrome (Yan, Free Radical Biol Med, doi:10.1016/j.freeradbiomed.2018.04.557; de la Vega Curr Pharmacol Rep, doi:10.1007/s40495-016-0053-2), pulmonary fibrosis, including idiopathic pulmonary fibrosis (Kikuchi, Respir Res, doi:10.1186/1465-9921-11- 31) and cystic fibrosis (Chen, PLoS One, doi:10.1371/journal.
• oxidative stress components Cho, Toxicol Appl Pharmacol, doi
• Nrf2 activators have highlighted their potential for the treatment of COVID-19 (Cuadrado et al, Trends Pharmacol Sci, doi :10.1016/j. tips.2020.07.003).
• Nrf2 The cardiac protective nature of Nrf2 in models of atherosclerosis, ischaemia, reperfusion, cardiac hypertrophy and heart failure has been demonstrated (Chen, Physiol Genomics, doi:10.1152/physiolgenomics.00041.2017).
• the Nrf2 activator Bardoxolone methyl has recently completed a Phase II study in patients with pulmonary arterial hypertension (PAH), with a Phase III study underway based on significant improvements in 6 minute walking distance.
• PAH pulmonary arterial hypertension
• CTD connective tissue disorder
• Nrf2 activation has been shown to suppress myocardial oxidative stress, cardiac apoptosis, hypertrophy, fibrosis and dysfunction in mouse models of pressure overload (Wang, J Card Failure, doi:10.1016/j.cardfail.2012.06.003) and to protect against cardiac ischemic/reperfusion injury in rodent models (Zhang, J Mol Cell Cardiol, doi:10.1016/j.yjmcc.2010.05.01 ).
• Nrf2 activator sulforaphane reduces hepatic glucose production and improves glucose control in patients with type 2 diabetes (Axelsson, Sci Transl Med., doi:
• Age-associated mitochondrial dysfunction and oxidative damage are primary causes for multiple health problems including sarcopenia and cardiovascular disease and treatment of aging mice with the Nrf2 activator Sulforaphane restored Nrf2 activity, mitochondrial function, cardiac function, exercise capacity, glucose tolerance, and activation/differentiation of skeletal muscle satellite cells (Bose et al, Aging cell, doi:10.1111/acel.13261 ).
• Nrf2 drugs leading to activation of Nrf2 should be useful in a number of cardiovascular and metabolic diseases including, but not limited to, atherosclerosis, hypertension, heart failure, myocardial infarction and repair, cardiac remodelling, cardiac arrhythmias, heart failure with reduced ejection fraction, diabetic cardiomyopathy, diabetic nephropathy, metabolic syndrome, obesity, diabetes mellitus (type 1 or type 2) and insulin resistance.
• Subarachnoid haemorrhage is a devastating condition with high morbidity and mortality rates due to the lack of effective therapy.
• EBI early brain injury
• CVS cerebral vasospasm
• SAH cerebral vasospasm
• AS Subarachnoid Haemorrhage
• Nrf2 activators rapidly increased HO-1 expression in astrocytes and reduced their vulnerability to haemoglobin or hemin.
• Systemic treatment with small molecule Nrf2 activators increased HO-1 expression in perivascular cells, particularly astrocytes.
• Nrf2 activators When tested in mouse or rat ICH models, Nrf2 activators were consistently protective, improving barrier function and attenuating edema, inflammation, neuronal loss and neurological deficits (Chen-Roetling, Curr Pharm Des, doi:10.2174/1381612822666161027150616). Ischemic stroke induces reactive oxygen species, causing oxidative and inflammatory responses in ischemic brain. To date, recombinant tissue plasminogen activator is the only available therapy for the treatment of ischemic stroke. However, the treatment does not prevent oxidative stress and inflammation in the ischemic brain.
• D3T a sulfur-containing dithiolethione compound
• D3T has been shown (Yen, J Immunol 2017, 198 (1 supplement) 206.20) to attenuate brain infarct and ameliorate neurological deficits in stroke animals.
• D3T reduced CNS infiltrating inflammatory immune cells including neutrophils and monocytes in the ischemic brain.
• D3T-induced suppression of inflammatory cytokine production was observed in wild -type but not in Nrf2- deficient microglia.
• Nrf2 is believed to play a key role in some hemoglobinopathies, such as beta- thalassemia and sickle cell disease (SCD).
• SCD is a recessive inherited disorder caused by a single missense mutation which leads to the mutated beta-globin protein haemoglobin S (HbS).
• HbS haemoglobin S
• Nrf2 activation has been shown to slow down the progression of haemolytic anemia and organ disfunction (Ghosh, JCI Insight, doi: 10.1172/jci.insight.81090) and loss of Nrf2 function worsens the pathophysiology of SCD in transgenic SCD mice (Zhu, Blood, doi.org/10.1182/blood-2017-10-810531 ).
• Nrf2 activators have also been shown to modulate foetal haemoglobin (HbF) expression through direct binding in the gamma-globin promoter and modification of chromatin structure in the beta-globin locus.
• HbF foetal haemoglobin
• Nrf2 provides unique benefits through HbF induction to inhibit haemoglobin S polymerization and protection against oxidative stress due to chronic haemolysis (Zhu et al, Exp Biol Med doi: 10.1177/1535370219825859).
• HbF haemoglobin S polymerization
• oxidative stress due to chronic haemolysis Zhu et al, Exp Biol Med doi: 10.1177/1535370219825859
• small molecule activators of Nrf2 has the potential to ameliorate the clinical severity of sickle cell disease and other diseases where increasing HbF is beneficial such as beta-thalassemia.
• Nrf2 The function of Nrf2 is altered in many neurodegene native disorders, such as Huntington’s disease, Parkinson’s Disease, Alzheimer’s disease, amyotrophic lateral sclerosis, frontotemporal dementia, multiple sclerosis and Friedreich's ataxia (Dinkova- Kostova, FEBS, doi:10.1111/febs.14379). Nrf2 activation mitigates multiple pathogenic processes involved in these neurodegenerative disorders through upregulation of antioxidant defences, inhibition of inflammation, improvement of mitochondrial function, and maintenance of protein homeostasis.
• Nrf2 small molecule pharmacological activators of Nrf2 have shown protective effects in numerous animal models of neurodegenerative diseases (Joshi, Neurobiol Aging, doi: 10.1016/j.neurobiolaging.2014.09.004; Alarcon- Aguilar, Neurobiol Aging, doi:10.1016/j.neurobiolaging.2014.01.143 and in cultures of human cells expressing mutant proteins.
• Tecfidera dimethyl fumarate activates Nrf2 (in addition to other mechanisms) and is approved in the US to treat relapsing-remitting multiple sclerosis.
• Nrf2 activator Omaveloxolone (RTA-408) currently in Phase II trials for the treatment of the inherited neurodegenerative disorder Friedrich’s ataxia, has met its primary endpoint of change in the modified Friedrich's Ataxia Rating Scale (mFARS) relative to placebo after 48 weeks of treatment.
• Targeting Nrf2 signalling may therefore provide a therapeutic option to delay onset, slow progression, and ameliorate symptoms of neurodegenerative disorders.
• Rheumatoid arthritis is an autoimmune disease that causes chronic inflammation of the joints and is characterized by periods of disease flares and remissions. Multiple joints can be affected sometimes resulting in permanent joint destruction and deformity.
• Nrf2 has been found to be activated in the joints of arthritic mice and of RA patients. Nrf2-knockout mice have more severe cartilage injuries and more oxidative damage, with the expression of Nrf2 target genes being enhanced in Nrf2 -wild-type but not in knockout mice during antibody-induced arthritis (Wruck, BMJ Annals of Rheumatic Diseases, doi:10.1136/ard.2010.132720).
• Nrf2(-/-) mice Nrf2(-/-) mice
• Nrf2 deficiency accelerated the incidence of arthritis, and animals showed a widespread disease affecting both front and hind paws (Maicas, Antioxidants & Redox Signaling, doi:10.1089/ars.2010.3835).
• Ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing-remitting forms of inflammatory bowel disease (IBD) that are caused by dysfunction of the intestinal epithelium. Damage to the intestinal epithelial cells can disrupt the barrier function of the intestinal epithelium, facilitating an aberrant immune response and inflammatory conditions. Thus, the intact intestinal epithelium is critical for the healthy gut, and cyto- protective agents that could target the intestinal epithelial cells would be beneficial for the treatment of DC and CD.
• IBD inflammatory bowel disease
• Antioxidant levels and oxidative stress bio markers are typically correlated with disease severity in IBD and a number of genome-wide association studies have linked IBD-associated single nucleotide polymorphisms (SNPs) to multiple genes involved in the response to oxidative stress, with many regulated by Nrf2 (Khor et al,
• Keap1-Nrf2 protein-protein interaction (and hence Nrf2 activator) has demonstrated a cyto protective effect in an experimental model of UC induced by dextran sodium sulphate in both NCM460 cells and mouse colon (Lu, Scientific Reports, doi:10.1038/srep26585). It has also been shown that Nrf2 knockout mice show an increased susceptibility to colitis- associated colorectal cancer (Khor, Cancer Prev Res (Phila), doi:10.1158/1940-6207).
• Nrf2 Activation of Nrf2 has been shown to have beneficial effects in diseases of both the liver and kidney.
• NAFLD Non-alcoholic fatty liver disease
• NAFLD represents a spectrum of diseases, some of which can progress to cirrhosis and hepatocellular carcinoma (HCC).
• HCC hepatocellular carcinoma
• NASH nonalcoholic steatohepatitis
• Nrf2 has been shown to reverse NASH in mouse models (Sharma, Cell Mol Gastroenterol Hepatol, doi:10.1016/j.jcmgh.2017.11.016).
• Nrf2 activators such as toxin -induced liver disease, viral hepatitis and cirrhosis.
• Oxidative-stress molecules such as reactive oxygen species, accumulate in the kidneys of animal models for acute kidney injury (AKI), in which Nrf2 is transiently and slightly activated.
• AKI acute kidney injury
• Nrf2 is transiently and slightly activated.
• Genetic or pharmacological enhancement of Nrf2 activity in the renal tubules significantly ameliorates damage related to AKI and prevents AKI progression to chronic kidney disease (CKD) by reducing oxidative stress.
• CKD chronic kidney disease
• FSGS focal segmental glomerulosclerosis
• CXA-10 which up regulates Nrf2 pathways is currently in clinical trials for Focal Segmental Glomerulosclerosis (FIRSTx - A Study of Oral CXA-10 in Primary Focal Segmental Glomerulosclerosis (FSGS); clinicaltrials.gov/ct2/show/NCT03422510).
• Bardoxolone methyl has been shown in a Phil trial to lead to significant improvement in kidney function in patients with either autosomal dominant polycystic kidney disease (ADPKD), CKD associated with type 1 diabetes (T 1 D), IgA nephropathy (IgAN) or FSGS after 12 weeks of treatment (https://www.reatapharma.com/press-releases/reata-announces-positive-phase-2-data- for-bardoxolone-methyl-in-patients-with-focal-segmental-glomerulosclerosis-and-in- patients-from-all-four-cohorts-of-phoenix/).
• ADPKD autosomal dominant polycystic kidney disease
• T 1 D type 1 diabetes
• IgAN IgA nephropathy
• FSGS after 12 weeks of treatment
• Alport Syndrome is the second most common inherited cause of kidney failure caused by a genetic defect in type IV collagen, a component in building the glomerular basement membrane.
• bardoxolone methyl is thought to affect the underlying pathologic processes associated with mitochondrial dysfunction, inflammation and oxidative stress, it is currently being studied in these patients suggesting Nrf2 activators will be potentially useful in this disease.
• Nrf2 Oxidative stress plays a critical role in the initiation and progression of cancer (Gorrini, Nat Rev Drug Discov., doi: 10.1038/nrd4002). Due to its importance in the maintenance of redox cellular homeostasis, Nrf2 is considered a cytoprotective transcription factor and tumour suppressor. At lower homeostatic levels Nrf2 is able to eliminate ROS, carcinogens and other DNA-damaging agents, leading to the inhibition of tumour initiation and metastasis (Milkovic et al. Redox Biol. doi:10.1016/j.redox.2017.04.013).
• Evgen is currently evaluating SFX-01 (sulforaphane- cyclodextrin complex) in the Treatment and Evaluation of Metastatic Breast Cancer (STEM) (clinicaltrials.gov/ct2/show/NCT02970682) which includes ER+/HER- metastatic breast cancer.
• STEM Metastatic Breast Cancer
• Bardoxolone derivatives have been shown to prevent lung cancer induced by vinyl carbamate in A/J mice (Liby, Cancer Res. doi: 10.1158/0008-5472).
• activators of Nrf2 may have a role in the prevention of cancer.
• Nrf2 activators are able to protect cells cultured to mimic the external layer of the retina from oxidative stress suggesting the potential for vision preservation in early AMD patients (Bellezza, Front Pharmacol. 2018; 9: 1280).
• Nrf2 activators may also be useful in other eye conditions such as Fuchs Endothelial Corneal Dystrophy and uveitis.
• Nrf-2 activators have also been suggested to have benefit for the treatment of preeclampsia via suppression of oxidative stress and endothelial cell apoptosis (Jiang et al, Oxid Med Cell Longev doi:10.1155/2021/8839394).
• the present invention provides a compound, or a pharmaceutically acceptable salt thereof as defined herein.
• the present invention provides a pharmaceutical composition
• a pharmaceutical composition comprising a compound of the invention as defined herein, or a pharmaceutically acceptable salt thereof, and one or more pharmaceutically acceptable excipients.
• the present invention relates to a compound of the invention as defined herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein, for use in therapy.
• the present invention relates to a compound of the invention as defined herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein, for use in the treatment of diseases or disorders mediated by Nrf2 activation.
• the present invention relates to the use of a compound of the invention as defined herein, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of diseases or disorders mediated by Nrf2 activation.
• the present invention relates to a method of treating a disease or disorder mediated by Nrf2 activation, said method comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound of the invention as defined herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein.
• Nrf2 activation examples include chronic obstructive pulmonary disease, asthma, pulmonary arterial hypertension, diabetes mellitus, chronic kidney disease, ulcerative colitis, Crohn's disease, inflammatory bowel disease, Friedreich's ataxia, sickle cell disease and non-alcoholic steatohepatitis.
• the present invention provides a compound, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein, for use in the treatment of chronic obstructive pulmonary disease, asthma, pulmonary arterial hypertension, diabetes mellitus, chronic kidney disease, ulcerative colitis, Crohn's disease, inflammatory bowel disease, Friedreich's ataxia, sickle cell disease or non-alcoholic steatohepatitis.
• the present invention provides the use of a compound, or a pharmaceutically acceptable salt, in the manufacture of a medicament for use in the treatment of chronic obstructive pulmonary disease, asthma, pulmonary arterial hypertension, diabetes mellitus, chronic kidney disease, ulcerative colitis, Crohn's disease, inflammatory bowel disease, Friedreich's ataxia, sickle cell disease or non-alcoholic steatohepatitis.
• the present invention provides a method of treating chronic obstructive pulmonary disease, asthma, pulmonary arterial hypertension, diabetes mellitus, chronic kidney disease, ulcerative colitis, Crohn's disease, inflammatory bowel disease, Friedreich's ataxia, sickle cell disease or non-alcoholic steatohepatitis, said method comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein.
• the present invention further provides a method of synthesising a compound, or a pharmaceutically acceptable salt thereof, as defined herein.
• the present invention provides a compound, or a pharmaceutically acceptable salt thereof, obtainable by, or obtained by, or directly obtained by a method of synthesis as defined herein.
• the present invention provides novel intermediates as defined herein which are suitable for use in any one of the synthetic methods set out herein.
• references to “treating” or “treatment” include prophylaxis as well as the alleviation of established symptoms of a condition.
• “Treating” or “treatment” of a state, disorder or condition therefore includes: (1) preventing or delaying the appearance of clinical symptoms of the state, disorder or condition developing in a human that may be afflicted with or predisposed to the state, disorder or condition but does not yet experience or display clinical or subclinical symptoms of the state, disorder or condition, (2) inhibiting the state, disorder or condition, i.e., arresting, reducing or delaying the development of the disease or a relapse thereof (in case of maintenance treatment) or at least one clinical or subclinical symptom thereof, or (3) relieving or attenuating the disease, i.e., causing regression of the state, disorder or condition or at least one of its clinical or subclinical symptoms.
• a “therapeutically effective amount” means the amount of a compound that, when administered to a mammal for treating a disease, is sufficient to effect such treatment for the disease.
• the “therapeutically effective amount” will vary depending on the compound, the disease and its severity and the age, weight, etc., of the mammal to be treated.
• alkyl includes both straight and branched chain alkyl groups. References to individual alkyl groups such as “propyl” are specific for the straight chain version only and references to individual branched chain alkyl groups such as “isopropyl” are specific for the branched chain version only.
• (1-6C)alkyl includes (1-4C)alkyl, (1-3C)alkyl, propyl, isopropyl and t-butyl.
• phenyl(1 -6C)alkyl includes phenyl(1 -4C)alkyl, benzyl, 1-phenylethyl and 2-phenylethyl.
• alkylene includes both straight and branched chain divalent alkyl groups.
• C 1-4 alkylene includes methylene (-CH2-), ethylene (- CH 2 CH 2 -), propylene and butylene.
• alkoxy includes both straight and branched chain alkyl groups singularly bonded to oxygen.
• C 1-4 alkoxy includes methoxy, ethoxy, isopropoxy and t-butoxy.
• (m-nC) or "(m-nC) group” used alone or as a prefix, refers to any group having m to n carbon atoms.
• Cycloalkyl means a hydrocarbon monocyclic or bicyclic ring containing carbon atoms.
• monocyclic cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl.
• Bicyclic rings may be fused or spiro attached; examples of bicyclic cycloalkyl groups include bicyclo[2.2.2]octane, bicyclo[2.1 .1]hexane, bicyclo[1.1.1]pentane, spiro[2.4]heptane, bicyclo[4.1.0]heptane and bicyclo[2.2.1]heptane.
• halo refers to fluoro, chloro, bromo and iodo.
• haloalkyl is used herein to refer to an alkyl group respectively in which one or more hydrogen atoms have been replaced by halogen (e.g. fluorine) atoms.
• halogen e.g. fluorine
• haloalkyl groups include fluoroalkyl groups such as -CHF 2 , -CH 2 CF 3 , or perfluoroalkyl/alkoxy groups such as -CF 3 , or -CF 2 CF 3 .
• heterocyclyl means a non -aromatic saturated or partially saturated monocyclic, fused, bridged, or spiro bicyclic heterocyclic ring system(s).
• Monocyclic heterocyclic rings contain from about 3 to 12 (suitably from 3 to 7) ring atoms, with from 1 to 5 (suitably 1 , 2 or 3) heteroatoms selected from nitrogen, oxygen or sulfur in the ring.
• Bicyclic heterocycles contain from 7 to 17 member atoms, suitably 7 to 12 member atoms, in the ring.
• Bicyclic heterocyclic(s) rings may be fused, spiro, or bridged ring systems.
• heterocyclic groups include cyclic ethers such as oxiranyl, oxetanyl, tetrahydrofuranyl, dioxanyl, and substituted cyclic ethers.
• Heterocycles containing nitrogen include, for example, azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, tetrahydrotriazinyl, tetrahydropyrazolyl, and the like.
• Typical sulfur containing heterocycles include tetrahydrothienyl, dihydro-1 ,3-dithiol, tetrahydro-2H-thiopyran, and hexahydrothiepine.
• heterocycles include dihydro-oxathiolyl, dihydroisoxazolyl (such as 4,5- dihydroisoxazolyl), dihydropyridinyl (such as 1 ,2-dihyd ropy rid i nyl or 1 ,6-dihydropyridinyl), tetrahyd ro-oxazoly I, tetrahyd ro-oxadiazolyl, tetrahydro-dioxazolyl, tetrahyd ro-oxathiazolyl, hexahydrotriazinyl, tetrahydro-oxazinyl, morpholinyl, thiomorpholinyl, tetrahydropyrimidinyl, dioxolinyl, octahydrobenzofuranyl, octahydrobenzimidazolyl, and octahydrobenzothiazo
• the oxidized sulfur heterocycles containing SO or SOz groups are also included.
• examples include the sulfoxide and sulfone forms of tetrahydrothienyl and thiomorpholinyl such as tetrahydrothiene 1 ,1 -dioxide and thiomorpholinyl 1 ,1 -dioxide.
• heterocyclyl groups are saturated monocyclic 3 to 7 membered heterocyclyls containing 1 , 2 or 3 heteroatoms selected from nitrogen, oxygen or sulfur, for example azetidinyl, tetra hyd rof u ranyl , tetrahyd ropy ranyl, pyrrolidinyl, morpholinyl, tetrahydrothienyl, tetrahydrothienyl 1 ,1 -dioxide, thiomorpholinyl, thiomorpholinyl 1,1 -dioxide, piperidinyl, homopiperidinyl, piperazinyl or homopiperazinyl.
• heterocycle may be linked to another group via any suitable atom, such as via a carbon o r nitrogen atom.
• heterocyclyl will refer to 4, 5, 6 or 7 membered monocyclic rings as defined above.
• C 1-4 alkylene-C 3-7 cycloalkyl refers to C- 1-4 alkylene substituted by a C 3-7 cycloalkyl group.
• the present invention provides a compound of Formula I wherein:
• R 1 is C i-4 alkylene-R 4 ;
• R 2 is selected from CO 2 H and tetrazolyl
• R 3 is selected from hydrogen and methyl
• X is CR 5 R 6 or O
• R 4 is 1 ,2,3-triazolyl, 1 ,2,4-triazolyl or pyrimidinyl, wherein said triazolyl or pyrimidinyl group is: optionally substituted with one or more substituents independently selected from C 1-4 alkyl, C 1-3 loalkyl, C 3-7 cycloalkyl, C 1-4 alkylene-C 3-7 cycloalkyl, halo, OH, C 1- 3alkoxy, and cyano; and optionally fused to a cycloalkyl or heterocyclyl ring;
• R 5 is selected from hydrogen, C 1-4 alkyl, C 3-7 cycloalkyl and C 1-3 haloalkyl;
• R 6 is selected from hydrogen and C 1-4 alkyl
• R 5 and R 6 together with the carbon atom to which they are attached, form a 3- or 4- membered cycloalkyl ring; m is 0 or 1 ; n is 1 or 2; or a pharmaceutically acceptable salt thereof; provided that the compound of formula I is not one of the following compounds:
• Particular compounds of the invention include, for example, compounds of the formula I, or pharmaceutically acceptable salts thereof, wherein, unless otherwise stated, each of R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , X, n and m has any of the meanings defined hereinbefore or in any of paragraphs (1 ) to (54) hereinafter.
• the scope of the present invention encompasses compounds of formula I, or pharmaceutically acceptable salts thereof, wherein any of the substituent definitions defined herein may be combined with any of the other substituent definitions also defined herein :-
• R 1 is C 1-4 alkylene-R 4 ;
• R 1 is CH 2 -R 4 ;
• R 1 is CH 2 CH 2 -R 4 ;
• R 2 is CO 2 H
• R 2 is tetrazolyl
• R 3 is hydrogen; (7) R 3 is methyl;
• R 4 is 1 ,2,3-triazolyl optionally substituted with one or more substituents independently selected from C 1-4 alkyl, Ci-shaloalkyl, C 3-7 cycloalkyl, C 1-4 alkylene-C 3 - 7cycloalkyl, halo, OH, C 1-3 alkoxy, and cyano;
• R 4 is 1 ,2,3-triazolyl optionally substituted with one or more substituents independently selected from C 1-4 alkyl, Ci-shaloalkyl, C 3-7 cycloalkyl and C 1-4 alkylene -C 3-7 cycloaIkyI;
• R 4 is 1 ,2,3-triazolyl optionally substituted with one or more substituents independently selected from C 1-4 alkyl and C 1-3 fluoroalkyl;
• R 4 is 1 ,2,3-triazolyl substituted with one or more substituents independently selected from methyl, difluoromethyl and trifluoromethyl;
• R 4 is 1 ,2,3-triazolyl-4-yl optionally substituted with one or more substituents independently selected from C 1-4 alkyl, C 1-3 haloalkyl, C 3-7 cycloalkyl, C 1-4 alkylene-C 3 - 7cycloalkyl, halo, OH, C 1-3 alkoxy, and cyano;
• R 4 is 1 H-1 ,2,3-triazolyl-4-yl optionally substituted with one or more substituents independently selected from methyl, difluoromethyl and trifluoromethyl;
• R 4 is 1 -methyl-1 H-1 ,2,3-triazolyl-4-yl optionally substituted with C 1-4 alkyl, C 1- 3 haloalkyl, C 3-7 cycloalkyl, C 1-4 alkylene- C 3-7 cycloalkyl, halo, OH, C 1-3 alkoxy or cyano;
• R 4 is 1 -methyl-1 H-1 ,2,3-triazolyl-4-yl optionally substituted with methyl, difluoromethyl or trifluoromethyl;
• R 4 is 1 ,2,4-triazolyl optionally substituted with one or more substituents independently selected from C 1-4 alkyl, C 1-3 haloalkyl, C 3-7 cycloalkyl, C 1-4 alkylene-C 3 - 7cycloalkyl, halo, OH, C 1-3 alkoxy, and cyano;
• R 4 is 1 ,2,4-triazolyl optionally substituted with one or more substituents independently selected from C 1-4 alky Il and C 1-3 fluoroalkyl;
• R 4 is 1 ,2,4-triazolyl substituted with one or more substituents independently selected from methyl, difluoromethyl and trifluoromethyl;
• R 4 is 4H-1 ,2,4-triazol-3-yl optionally substituted with one or more substituents independently selected C 1-4 alkyl, C 1-3 haloalkyl, C 3-7 cycloalkyl, C 1-4 alkylene- C 3-7 cycloalkyl , halo, OH, C 1-3 alkoxy and cyano;
• R 4 is 4H-1 ,2,4-triazol-3-yl optionally substituted with one or more substituents independently selected from methyl, difluoromethyl and trifluoromethyl;
• R 4 is 4-methyl-4H-1 ,2,4-triazol-3-yl optionally substituted with C 1-4 alkyl, C 1-3 haloalkyl , C 3-7 cycloalkyl, C 1-4 alkylene-C 3-7 cycloalkyl, halo, OH, C 1-3 alkoxy or cyano;
• R 4 is 4-methyl-4H-1 ,2,4-triazol-3-yl optionally substituted with methyl, difluoromethyl or trifluoromethyl;
• R 4 is pyrimidinyl optionally substituted with one or more substituents independently selected from C 1-4 alkyl, C 1-3 haloalkyl, C 3-7 cycloalkyl, C 1-4 alkylene-C 3-7 cycloalkyl, halo, OH, C 1-3 alkoxy, and cyano;
• R 4 is pyrimidinyl optionally substituted with one or more substituents independently selected from C- 1-4 alkyl and C 1-3 haloalkyl;
• R 4 is pyrimidinyl optionally substituted with one or more substituents independently selected from methyl, difluoromethyl and trifluoromethyl;
• R 4 is pyrimidin-5-yl optionally substituted with one or more substituents independently selected from C 1-4 alkyl, Ci-shaloalkyl, C 3-7 cycloalkyl, C 1-4 alkylene-C 3- 7 cycloalkyl, halo, OH, C 1-3 alkoxy or cyano;
• R 4 is pyrimidin-5-yl optionally substituted with methyl, difluoromethyl or trifluoromethyl;
• R 4 is 1 ,2,3-triazolyl fused to a cycloalkyl or heterocyclyl ring;
• R 4 is 1 ,2,3-triazol-4-yl fused to a cycloalkyl or heterocyclyl ring;
• R 4 is selected from one of the following groups: wherein said group is optionally substituted with C 1-4 alkyl and C 1-3 fluoroalkyl; (31 ) R 4 is selected from one of the following groups: wherein said group is optionally substituted with methyl or difluoromethyl; (32) R 4 is selected from one of the following groups: wherein said group is optionally further substituted with C 1-4 alkyl and C 1-3 fluoroalkyl;
• R 4 is selected from one of the following groups: wherein said group is optionally further substituted with methyl, difluoromethyl or trifluoromethyl;
• R 4 is selected from one of the following groups:
• R 1 is CH 2 -R 4 and R 4 is selected from one of the following groups: wherein said group is optionally substituted with C 1-4 alky Il and C 1-3 fluoroalkyl;
• R 1 is CH 2 -R 4 and R 4 is selected from one of the following groups: wherein said group is optionally further substituted with C 1-4 alkyl and C 1-3 fluoroalkyl;
• R 1 is CH 2 -R 4 and R 4 is selected from one of the following groups: wherein said group is optionally further substituted with methyl, difluoromethyl or trifluoromethyl;
• R 1 is CH 2 -R 4 and R 4 is selected from one of the following groups: (39) R 1 is CH 2 -R 4 and R 4 is selected from one of the following groups:
• X is CR 5 R 6 ; (42) R 5 is selected from hydrogen, methyl, cyclopropyl and Ci-gfluoroalkyl;
• R 5 is selected from hydrogen and methyl
• R 5 is methyl
• R 6 is selected from hydrogen and methyl
• R 6 is hydrogen; (47) R 5 is methyl and R 6 is hydrogen;
• n is 1 and X is CR 5 R 6 ; (50) n is 1 , X is CR 5 R 6 , R 5 is methyl and R 6 is hydrogen;
• n 1
• X is CR 5 R 6 and R 5 and R 6 , together with the carbon atom to which they are attached, form a cyclopropyl ring;
• n 2 and X is O; (53) m is 0;
• R 1 is as defined in any one of paragraphs (1) to (3) or (35) to (39) above. In an embodiment, R 1 is as defined in paragraph (2) above. In an embodiment, R 1 is as defined in paragraph (39) above.
• R 2 is as defined in any one of paragraphs (4) to (5) above. In an embodiment, R 2 is as defined in paragraph (4) above.
• R 3 is as defined in any one of paragraphs (6) to (7) above. In an embodiment, R 3 is as defined in paragraph (7) above.
• R 4 is as defined in any one of paragraphs (8) to (34) above. In an embodiment, R 4 is as defined in paragraphs (32) to (34) above. In an embodiment, R 4 is as defined in paragraph (34) above.
• X is as defined in any one of paragraphs (40) to (41) above. In an embodiment, X is as defined in paragraph (41 ) above.
• R 5 is as defined in any one of paragraphs (42) to (44) above. In an embodiment, R 5 is as defined in paragraph (44) above.
• R 6 is as defined in any one of paragraphs (45) to (46) above. In an embodiment, R 6 is as defined in paragraph (46) above.
• R 5 and R 6 are as defined in any one of paragraphs (47) to (48) above. In an embodiment, R 5 and R 6 are as defined in paragraph (48) above.
• n is as defined in any one of paragraphs (49) to (52) above. In an embodiment, n is as defined in paragraph (49) above.
• m is as defined in any one of paragraphs (53) to (54) above. In an embodiment, m is as defined in paragraph (54) above.
• the compounds have one of the structural formulae IA to IK (which are sub-formulae of Formula I) shown below, or a pharmaceutically acceptable salt thereof:
• R 1 to R 6 , X and m are as defined hereinbefore.
• the compounds have the structural formula IA shown above, wherein R 1 is as defined in any one of paragraphs (1 ) to (3) above; R 2 is as defined in any one of paragraphs (4) to (5) above; R 3 is as defined in any one of paragraphs (6) to (7) above; and X is as defined in any one of paragraphs (40) to (41 ) above; provided that the compound of formula IA is not one of the following compounds:
• the compounds have the structural formula IA shown above, wherein R 1 is as defined in paragraph (2) above; R 2 is as defined in paragraph (4) above; R 3 is as defined in paragraph (7) above; and X is as defined paragraph (41 ) above.
• the compounds have the structural formula IB shown above, wherein R 1 is as defined in any one of paragraphs (1) to (3) above; R 2 is as defined in any one of paragraphs (4) to (5) above; R 3 is as defined in any one of paragraphs (6) to (7) above; and X is as defined in any one of paragraphs (40) to (41) above.
• the compounds have the structural formula IB shown above, wherein R 1 is as defined in paragraph (2) above; R 2 is as defined in paragraph (4) above; R 3 is as defined in paragraph (7) above; and X is as defined paragraph (41 ) above.
• the compounds have the structural formula IC shown above, wherein R 1 is as defined in any one of paragraphs (1) to (3) above; R 2 is as defined in any one of paragraphs (4) to (5) above; R 3 is as defined in any one of paragraphs (6) to (7) above; and m is as defined in any one of paragraphs (53) to (54) above.
• the compounds have the structural formula IC shown above, wherein R 1 is as defined in paragraph (2) above; R 2 is as defined in paragraph (4) above; R 3 is as defined in paragraph (7) above; and m is as defined paragraph (54) above.
• the compounds have the structural formula ID shown above, wherein R 1 is as defined in any one of paragraphs (1 ) to (3) above; R 2 is as defined in any one of paragraphs (4) to (5) above; R 3 is as defined in any one of paragraphs (6) to (7) above; R 5 is as defined in any one of paragraphs (42) to (44) above, R 6 is as defined in any one of paragraphs (45) to (46) above; or R 5 and R 6 are as defined in any one of paragraphs (47) to (48) above; and m is as defined in any one of paragraphs (53) to (54) above; provided that the compound of formula ID is not one of the following compounds:
• the compounds have the structural formula ID shown above, wherein R 1 is as defined in paragraph (2) above; R 2 is as defined in paragraph (4) above; R 3 is as defined in paragraph (7) above; R 5 is as defined in paragraph (44) above, R 6 is as defined in paragraph (46) above; or R 5 and R 6 are as defined in paragraph (48) above; and m is as defined in paragraph (54) above.
• the compounds have the structural formula IE shown above, wherein R 1 is as defined in any one of paragraphs (1 ) to (3) above; R 3 is as defined in any one of paragraphs (6) to (7) above; R 5 is as defined in any one of paragraphs (42) to (44) above, R 6 is as defined in any one of paragraphs (45) to (46) above; or R 5 and R 6 are as defined in any one of paragraphs (47) to (48) above; provided that the compound of formula IE is not one of the following compounds:
• the compounds have the structural formula IE shown above, wherein R 1 is as defined in paragraph (2) above; R 3 is as defined in paragraph (7) above; R 5 is as defined in paragraph (44) above, R 6 is as defined in paragraph (46) above; or R 5 and R 6 are as defined in paragraph (48) above.
• the compounds have the structural formula IF shown above, wherein R 2 is as defined in any one of paragraphs (4) to (5) above; R 3 is as defined in any one of paragraphs (6) to (7) above; R 4 is as defined in any one of paragraphs (8) to (34) above; R 5 is as defined in any one of paragraphs (42) to (44) above, R 6 is as defined in any one of paragraphs (45) to (46) above; or R 5 and R 6 are as defined in any one of paragraphs (47) to (48) above; and m is as defined in any one of paragraphs (53) to (54) above; provided that the compound of formula IF is not one of the following compounds:
• the compounds have the structural formula IF shown above, wherein R 2 is as defined in paragraph (4) above; R 3 is as defined in paragraph (7) above; R 4 is as defined in paragraph (34) above; R 5 is as defined in paragraph (44) above, R 6 is as defined in paragraph (46) above; or R 5 and R 6 are as defined in paragraph (48) above; and m is as defined in paragraph (54) above.
• the compounds have the structural formula IG shown above, wherein R 3 is as defined in any one of paragraphs (6) to (7) above; R 4 is as defined in any one of paragraphs (8) to (34) above; R 5 is as defined in any one of paragraphs (42) to (44) above, R 6 is as defined in any one of paragraphs (45) to (46) above; or R 5 and R 6 are as defined in any one of paragraphs (47) to (48) above; provided that the compound of formula IG is not one of the following compounds:
• the compounds have the structural formula IG shown above, wherein R 3 is as defined in paragraph (7) above; R 4 is as defined in paragraph (34) above; R 5 is as defined in paragraph (44) above, R 6 is as defined in paragraph (46) above; or R 5 and R 6 are as defined in paragraph (48) above.
• the compounds have the structural formula IH shown above, wherein R 4 is as defined in any one of paragraphs (8) to (34) above; R 5 is as defined in any one of paragraphs (42) to (44) above, R 6 is as defined in any one of paragraphs (45) to (46) above; or R 5 and R 6 are as defined in any one of paragraphs (47) to (48) above; and m is as defined in any one of paragraphs (53) to (54) above; provided that the compound of formula IH is not one of the following compounds:
• the compounds have the structural formula IH shown above, wherein R 4 is as defined in paragraph (34) above; R 5 is as defined in paragraph (44) above, R 6 is as defined in paragraph (46) above; or R 5 and R 6 are as defined in paragraph (48) above; and m is as defined in paragraph (54) above.
• the compounds have the structural formula IJ shown above, wherein R 4 is as defined in any one of paragraphs (8) to (34) above; and m is as defined in any one of paragraphs (53) to (54) above.
• the compounds have the structural formula IJ shown above, wherein R 4 is as defined in any one of paragraphs (32) to (34), such as in paragraph (34) above; and m is as defined in paragraph (54) above.
• the compounds have the structural formula IK shown above, wherein R 2 is as defined in any one of paragraphs (4) to (5) above; R 4 is as defined in any one of paragraphs (8) to (34) above; and m is as defined in any one of paragraphs (53) to (54) above; provided that the compound of formula IK is not one of the following compounds: (1S,2R)-2-((S)-5-chloro-8-((1 ,5-dimethyl-1 H- 1 ,2,3-triazol-4-yl)methoxy)-7-fluoro-1 -((6- oxo-5-azaspiro[2.4]heptan-5-yl)methyl)-1 ,2,3,4-tetrahydroisoquinoline-2-carbonyl)-1- methylcyclohexane-1 -carboxylic acid;
• the compounds have the structural formula IK shown above, wherein R 2 is as defined in paragraph (4) above; R 4 is as defined in paragraph (34) above; and m is as defined in paragraph (54) above.
• the compounds have the structural formula IL (which is a sub-formula of Formula I) shown below, or a pharmaceutically acceptable salt thereof: wherein R 5 and R 6 are as defined hereinbefore and R 7 and R 8 are independently selected from hydrogen, C 1-4 alkyl, C 1-3 haloalkyl, C 3-7 cycloalkyl, C 1-4 alkylene-C 3-7 cycloalkyl, halo, OH, C 1-3 alkoxy and cyano; or R 7 and R 8 , taken together with the atoms to which they are attached, form a 5- or 6-membered heterocyclyl ring; provided that the compound of formula IL is not one of the following compounds:
• the compounds have the structural formula IL shown above, wherein R 5 is as defined in any one of paragraphs (42) to (44) above, R 6 is as defined in any one of paragraphs (45) to (46) above; or R 5 and R 6 are as defined in any one of paragraphs (47) to (48) above; and R 7 and R 8 are independently selected from hydrogen, C 1-4 alkyl, C 1-3 haloalkyl, C 3-7 cycloalkyl and C 1-2 alkylene-C 3-7 cycloalkyl ; or R 7 and R 8 , taken together with the atoms to which they are attached, form a 6-membered heterocyclyl ring.
• the compounds have the structural formula IL shown above, wherein R 5 is as defined in paragraph (44) above, R 6 is as defined in paragraph (46) above; or R 5 and R 6 are as defined in paragraph (48) above; and R 7 and R 8 are independently selected from methyl, ethyl, difluoro methyl, trifluoro methyl, cyclopropyl and CH 2 -cyclopropyl; or R 7 and R 8 , taken together with the atoms to which they are attached, form a 6-membered heterocyclyl ring.
• the compounds have the structural formula IL shown above, wherein R 5 is as defined in paragraph (44) above, R 6 is as defined in paragraph (46) above; or R 5 and R 6 are as defined in paragraph (48) above; R 7 is methyl; and R 8 is hydrogen, methyl, or difluoromethyl.
• Particular compounds of the invention include any one of the following:
• the compound of formula I is selected from the following compounds:
• the various functional groups and substituents making up the compounds of the present invention are typically chosen such that the molecular weight of the compound does not exceed 1000. More usually, the molecular weight of the compound will be less than 750, for example less than 700, or less than 650.
• Suitable or preferred features of any compounds of the present invention may also be suitable features of any other aspect.
• a suitable pharmaceutically acceptable salt of a compound of the invention is, for example, an acid-addition salt of a compound of the invention which is sufficiently basic, for example, an acid-addition salt with, for example, an inorganic or organic acid, for example hydrochloric, hydrobromic, sulfuric, phosphoric, trifluoroacetic, formic, citric or maleic acid.
• a suitable pharmaceutically acceptable salt of a compound of the invention which is sufficiently acidic is an alkali metal salt, for example a sodium or potassium salt, an alkaline earth metal salt, for example a calcium or magnesium salt, an ammonium salt or a salt with an organic base which affords a physiologically-acceptable cation, for example a salt with methylamine, dimethylamine, trimethylamine, piperidine, morpholine or tris-(2-hydroxyethyl)amine.
• an alkali metal salt for example a sodium or potassium salt
• an alkaline earth metal salt for example a calcium or magnesium salt
• an ammonium salt or a salt with an organic base which affords a physiologically-acceptable cation
• a salt with methylamine, dimethylamine, trimethylamine, piperidine, morpholine or tris-(2-hydroxyethyl)amine for example a salt with methylamine, dimethylamine, trimethylamine, piperidine, morpholine or tris-(2-hydroxye
• isomers Compounds that have the same molecular formula but differ in the nature or sequence of bonding of their atoms or the arrangement of their atoms in space are termed “isomers”. Isomers that differ in the arrangement of their atoms in space are termed “stereoisomers”. Stereoisomers that are not mirror images of one another are termed “diastereomers” and those that are non-superimposable mirror images of each other are termed “enantiomers”. When a compound has an asymmetric centre, for example, it is bonded to four different groups, a pair of enantiomers is possible.
• An enantiomer can be characterized by the absolute configuration of its asymmetric centre and is described by the R- and S-sequencing rules of Cahn and Prelog, or by the manner in which the molecule rotates the plane of polarized light and designated as dextrorotatory or levorotatory (i.e., as (+) or (-)-isomers respectively).
• a chiral compound can exist as either individual enantiomer or as a mixture thereof. A mixture containing equal proportions of the enantiomers is called a “racemic mixture”.
• the compounds of this invention typically possess one or more asymmetric centers; such compounds can therefore be produced as individual (R)- or (S)-stereoisomers or as mixtures thereof. Unless indicated otherwise, the description or naming of a particular compound in the specification and claims is intended to include both individual enantiomers, diastereoisomers and mixtures, racemic or otherwise, thereof.
• the methods for the determination of stereochemistry and the separation of stereoisomers are well-known in the art (see discussion in Chapter 4 of “Advanced Organic Chemistry”, 4th edition J. March, John Wiley and Sons, New York, 2001), for example by synthesis from optically active starting materials or by resolution of a racemic form.
• Some of the compounds of the invention may have geometric isomeric centres (E- and Z- isomers). It is to be understood that the present invention encompasses all optical, diastereoisomers and geometric isomers and mixtures thereof that possess Nrf2 activation activity.
• the present invention also encompasses compounds of the invention as defined herein which comprise one or more isotopic substitutions.
• H may be in any isotopic form, including 1 H, 2 H (D) and 3 H (T);
• C may be in any isotopic form including 12 C, 13 C, and 14 C;
• O may be in any isotopic form, including 16 0 and 18 0; and the like.
• tautomeric forms include keto-, enol-, and enolate-forms, as in, for example, the following tautomeric pairs: keto/enol (illustrated below), imine/enamine, amide/imino alcohol, amidine/amidine, nitroso/oxime, thioketone/enethiol, and nitro/aci-nitro.
• N- oxides Compounds of the invention containing an amine function may also form N- oxides.
• a reference herein to a compound of the formula I that contains an amine function also includes the N- oxide.
• one or more than one nitrogen atom may be oxidised to form an N-oxide.
• Particular examples of N- oxides are the N-oxides of a tertiary amine or a nitrogen atom of a nitrogen-containing heterocycle.
• N- Oxides can be formed by treatment of the corresponding amine with an oxidizing agent such as hydrogen peroxide or a per-acid (e.g. a peroxycarboxylic acid), see for example Advanced Organic Chemistry, by Jerry March, 4 th Edition, Wiley Interscience, pages.
• N-oxides can be made by the procedure of L. W. Deady ( Syn . Comm. 1977, 7, 509-514) in which the amine compound is reacted with m- chloroperoxybenzoic acid (MCPBA), for example, in an inert solvent such as dichloromethane.
• MCPBA m- chloroperoxybenzoic acid
• the compounds of the invention may be administered in the form of a pro-drug which is broken down in the human or animal body to release a compound of the invention.
• a pro-drug may be used to alter the physical properties and/or the pharmacokinetic properties of a compound of the invention.
• a pro-drug can be formed when the compound of the invention contains a suitable group or substituent to which a property-modifying group can be attached.
• Examples of pro-drugs include in vivo cleavable ester derivatives that may be formed at a carboxy group or a hydroxy group in a compound of the invention and in-vivo cleavable amide derivatives that may be formed at a carboxy group or an amino group in a compound of the invention.
• the present invention includes those compounds of the formula I as defined hereinbefore when made available by organic synthesis and when made available within the human or animal body by way of cleavage of a pro-drug thereof. Accordingly, the present invention includes those compounds of the formula I that are produced by organic synthetic means and also such compounds that are produced in the human or animal body by way of metabolism of a precursor compound, that is a compound of the formula I may be a synthetically-produced compound or a metabolically-produced compound.
• a suitable pharmaceutically acceptable pro-drug of a compound of the formula I is one that is based on reasonable medical judgement as being suitable for administration to the human or animal body without undesirable pharmacological activities and without undue toxicity.
• Various forms of pro-drug have been described, for example in the following documents: a) Methods in Enzymoloqy, Vol. 42, p. 309-396, edited by K. Widder, et al. (Academic Press, 1985); b) Design of Pro-drugs, edited by H. Bundgaard, (Elsevier, 1985); c) A Textbook of Drug Design and Development, edited by Krogsgaard -Larsen and H.
• Bundgaard Chapter 5 “Design and Application of Pro-drugs”, by H. Bundgaard p. 113-191 (1991); d) H. Bundgaard, Advanced Drug Delivery Reviews. 8, 1-38 (1992); e) H. Bundgaard, et a!., Journal of Pharmaceutical Sciences. 77. 285 (1988); f) N. Kakeya, et al.,., Chem. Phamn. Bull.. 32. 692 (1984); g) T. Higuchi and V. Stella, “Pro-Drugs as Novel Delivery Systems”, A.C.S. Symposium Series, Volume 14; and h) E. Roche (editor), “Bio reversible Carriers in Drug Design”, Pergamon Press,
• the in vivo effects of a compound of the formula I may be exerted in part by one or more metabolites that are formed within the human or animal body after administration of a compound of the formula I. As stated hereinbefore, the in vivo effects of a compound of the formula I may also be exerted by way of metabolism of a precursor compound (a pro-drug).
• compounds of the formula I may also be covalently linked (at any suitable position) to other groups such as, for example, solubilising moieties (for example, PEG polymers), moieties that enable them to be bound to a solid support (such as, for example, biotin-containing moieties), and targeting ligands (such as antibodies or antibody fragments).
• solubilising moieties for example, PEG polymers
• moieties that enable them to be bound to a solid support such as, for example, biotin-containing moieties
• targeting ligands such as antibodies or antibody fragments
• protecting groups see one of the many general texts on the subject, for example, “Protecting groups in Organic Synthesis (3 rd Ed), John Wiley & Sons, NY (1999)”, T. Greene & P. Wuts.
• Protecting groups may be removed by any convenient method described in the literature or known to the skilled chemist as appropriate for the removal of the protecting group in question, such methods being chosen so as to effect removal of the protecting group with the minimum disturbance of groups elsewhere in the molecule.
• reactants include, for example, groups such as amino, carboxy or hydroxy it may be desirable to protect the group in some of the reactions mentioned herein.
• a suitable protecting group for an amino or alkylamino group is, for example, an acyl group, for example an alkanoyl group such as acetyl, an alkoxycarbonyl group, for example a methoxycarbonyl, ethoxy carbonyl or terttbutoxycarbonyl group, an arylmethoxycarbonyl group, for example benzyloxycarbonyl, or an aroyl group, for example benzoyl.
• the deprotection conditions for the above protecting groups necessarily vary with the choice of protecting group.
• an acyl group such as an alkanoyl or alkoxycarbonyl group or an aroyl group may be removed by, for example, hydrolysis with a suitable base such as an alkali metal hydroxide, for example lithium or sodium hydroxide.
• a suitable base such as an alkali metal hydroxide, for example lithium or sodium hydroxide.
• an acyl group such as a tert-butoxy carbonyl group may be removed, for example, by treatment with a suitable acid as hydrochloric, sulfuric or phosphoric acid or trifluoroacetic acid and an arylmethoxycarbonyl group such as a benzyloxycarbonyl group may be removed, for example, by hydrogenation over a catalyst such as palladium-on-carbon, or by treatment with a Lewis acid for example BF 3 .OEt 2 .
• a suitable alternative protecting group for a primary amino group is, for example, a phthaloyl group which may be removed by treatment with an alkylamine, for example dimethylaminopropylamine, or with hydrazine.
• a cycloalkyl acid group may be activated for reaction with the amine of the tetrahydroisoquinoline (THIQ) scaffold.
• THIQ tetrahydroisoquinoline
• Typical amide coupling reagents such as HATU or GDI are used to effect acid activion.
• the order of steps can be altered compared with General Method A.
• the required ether is installed in a first step, again through conventional methods as described in the above General Method A.
• the phthalimide protecting group is removed in a second step and the amine is reacted with appropropriate reagents to install the desired lactam.
• the Boc protecting group is then removed and cycloalkyl amide moiety may then be introduced in a fifth step.
• the THIQ scaffold may be constructed according to the route outlined in Scheme 1 :
• Scheme 1 a) SOCI2, EtOAc; b) ammonium acetate, CH3NO2, AcOH; c) LiAIH4, THF; d) DIPEA, DCM; e) NCS, DMF; f) P2O5, sulfolane; g) NaBH(OAc) 3 , AcOH, DCM; h) BBr 3 , DCM; i) chiral SFC chromatography.
• R 2 is -COOH can be converted to tetrazolyl via reaction of the appropriate nitrile with an azide, as shown in Scheme 2.
• compositions which comprises a compound of the invention as defined hereinbefore, or a pharmaceutically acceptable salt thereof, and one or more pharmaceutically acceptable excipients, diluent or carrier.
• compositions of the invention may be prepared and packaged in bulk form wherein a safe and effective amount of a compound of the invention can be extracted and then given to the patient such as with powders or syrups.
• the pharmaceutical compositions of the invention may be prepared and packaged in unit dosage form wherein each physically discrete unit contains a safe and effective amount of a compound of the invention.
• the pharmaceutical compositions of the invention typically contain from 1 mg to 1000 mg.
• compositions of the invention may be in a form suitable for oral use (for example as tablets, capsules, caplets, pills, troches, powders, syrups, elixirs, suspensions, solutions, emulsions, sachets, and cachets), for topical use (for example as creams, ointments, lotions, solutions, pastes, sprays, foams, and gels), for transdermal administration such as via transdermal patches, for administration by inhalation (for example as a dry powders, aerosols, suspensions, and solutions), for administration by insufflation (for example as a finely divided powder) or for parenteral administration (for example as a sterile aqueous or oily solution for intravenous, subcutaneous, intramuscular, intraperitoneal or intramuscular dosing or as a suppository for rectal dosing).
• oral use for example as tablets, capsules, caplets, pills, troches, powders, syrups, elixirs, suspension
• pharmaceutically-accepta b le excipient means a pharmaceutically acceptable material, composition or vehicle involved in giving form or consistency to the pharmaceutical composition.
• Each excipient must be compatible with the other ingredients of the pharmaceutical composition when commingled such that interactions which would substantially reduce the efficacy of the compound of the invention when administered to a patient and interactions which would result in pharmaceutical compositions that are not pharmaceutically acceptable are avoided.
• each excipient must be of sufficiently high purity to render it pharmaceutically-acceptable.
• Suitable pharmaceutically-acceptable excipients will vary depending upon the particular dosage form chosen.
• suitable pharmaceutically-acceptable excipients may be chosen for a particular function that they may serve in the composition.
• certain pharmaceutically-acceptable excipients may be chosen for their ability to facilitate the production of uniform dosage forms.
• Certain pharmaceutically- acceptable excipients may be chosen for their ability to facilitate the production of stable dosage forms.
• Certain pharmaceutically-acceptable excipients may be chosen for their ability to facilitate the carrying or transporting of the compound or compounds of the invention once administered to the patient from one organ, or portion of the body, to another organ, or portion of the body.
• Certain pharmaceutically-acceptable excipients may be chosen for their ability to enhance patient compliance.
• Suitable pharmaceutically-acceptable excipients include the following types of excipients: diluents, fillers, binders, disintegrants, lubricants, glidants, granulating agents, coating agents, wetting agents, solvents, co-solvents, suspending agents, emulsifiers, sweeteners, flavoring agents, flavor masking agents, coloring agents, anticaking agents, humectants, chelating agents, plasticizers, viscosity increasing agents, antioxidants, preservatives, stabilizers, surfactants, and buffering agents.
• compositions of the invention are prepared using techniques and methods known to those skilled in the art. Some of the methods commonly used in the art are described in Remington's Pharmaceutical Sciences (Mack Publishing Company).
• the amount of active ingredient that is combined with one or more excipients to produce a single dosage form will necessarily vary depending upon the host treated and the particular route of administration.
• a formulation intended for oral administration to humans will generally contain, for example, from 0.5 mg to 0.5 g of active agent (more suitably from 0.5 to 100 mg, for example from 1 to 30 mg) compounded with an appropriate and convenient amount of excipients which may vary from about 5 to about 98 percent by weight of the total composition.
• the size of the dose for therapeutic or prophylactic purposes of a compound of the formula I will naturally vary according to the nature and severity of the conditions, the age and sex of the animal or patient and the route of administration, according to well-known principles of medicine.
• a daily dose in the range for example, 0.1 mg/kg to 75 mg/kg body weight is received, given if required in divided doses.
• lower doses will be administered when a parenteral route is employed.
• a dose in the range for example, 0.1 mg/kg to 30 mg/kg body weight will generally be used.
• a dose in the range for example, 0.05 mg/kg to 25 mg/kg body weight will be used.
• Oral administration may also be suitable, particularly in tablet form.
• unit dosage forms will contain about 0.5 mg to 0.5 g of a compound of this invention.
• the compounds of the invention or pharmaceutical composition comprising the active compound may be administered to a subject by any convenient route of administration, whether systemically/peripherally or topically (i.e. at the site of desired action).
• Routes of administration include, but are not limited to, oral (e.g., by ingestion); buccal; sublingual; transdermal (including, e.g., by a patch, plaster, etc.); transmucosal (including, e.g., by a patch, plaster, etc.); intranasal (e.g., by nasal spray); ocular (e.g., by eyedrops); pulmonary (e.g., by inhalation or insufflation therapy using, e.g., via an aerosol, e.g., through the mouth or nose); rectal (e.g., by suppository or enema); vaginal (e.g., by pessary); parenteral, for example, by injection, including subcutaneous, intraderma I, intramuscular, intravenous, intraarterial, intracardiac, intrathecal, intraspinal, intracapsular, subcapsular, intraorbital, intraperitoneal, intratracheal, sub
• a compound of the invention as defined herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein, is administered orally or via inhalation.
• the compounds of the invention are activators of Nrf2. As a consequence, they are potentially useful therapeutic agents for the treatment of diseases or conditions mediated by Nrf2 activation.
• the present invention relates to a compound of the invention as defined herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein, for use in therapy.
• the present invention relates to a compound of the invention as defined herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein, for use in the treatment of diseases or disorders mediated by Nrf2 activation.
• the present invention relates to the use of a compound of the invention as defined herein, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of diseases or disorders mediated by Nrf2 activation.
• the present invention relates to a method of treating a disease or disorders mediated by Nrf2 activation, said method comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound of the invention as defined herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein.
• Examples of particular diseases or conditions that the compounds of formula (I) and their pharmaceutically acceptable salts may be used to treat include, but are not limited to, any one of the following: chronic obstructive pulmonary disease, acute, chronic and severe asthma, acute lung injury/acute respiratory distress syndrome with or without accompanying multi organ dysfunction syndrome, pulmonary fibrosis including idiopathic pulmonary fibrosis, cystic fibrosis, COVID-19, diabetes, atherosclerosis, hypertension, heart failure, myocardial infarction and repair, cardiac remodelling, cardiac arrhythmias, cardiac hypertrophy, heart failure with preserved ejection fraction, diabetic cardiomyopathy, sarcopenia, obesity, metabolic syndrome, diabetes mellitus, insulin resistance, pulmonary arterial hypertension, subarachnoid haemorrhage, intracerebral haemorrhage, ischemic stroke, beta-thalassemia, sickle cell disease, rheumatoid arthritis, irritable bowel disorder, ulcerative colitis,
• the compounds of the invention may be used in the treatment of chronic obstructive pulmonary disease, asthma, pulmonary arterial hypertension, diabetes mellitus, chronic kidney disease, ulcerative colitis, Crohn's disease, inflammatory bowel disease, Friedreich's ataxia, sickle cell disease or non-alcoholic steatohepatitis.
• the present invention provides a compound, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein, for use in the treatment of chronic obstructive pulmonary disease, acute, chronic and severe asthma, acute lung injury/acute respiratory distress syndrome with or without accompanying multi organ dysfunction syndrome, pulmonary fibrosis including idiopathic pulmonary fibrosis, cystic fibrosis, COVID-19, diabetes, atherosclerosis, hypertension, heart failure, myocardial infarction and repair, cardiac remodelling, cardiac arrhythmias, cardiac hypertrophy, heart failure with preserved ejection fraction, diabetic cardiomyopathy, sarcopenia, obesity, metabolic syndrome, diabetes mellitus, insulin resistance, pulmonary arterial hypertension, subarachnoid haemorrhage, intracerebral haemorrhage, ischemic stroke, beta-thalassemia, sickle cell disease, rheumatoid arthritis, irritable bowel disorder, ulcerative colitis, Crohn'
• the present invention provides a compound, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein, for use in the treatment of chronic obstructive pulmonary disease, asthma, pulmonary arterial hypertension, diabetes mellitus, chronic kidney disease, Friedreich's ataxia, sickle cell disease or non-alcoholic steatohepatitis.
• the present invention provides the use of a compound, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of chronic obstructive pulmonary disease, acute, chronic and severe asthma, acute lung injury/acute respiratory distress syndrome with or without accompanying multi organ dysfunction syndrome, pulmonary fibrosis including idiopathic pulmonary fibrosis, cystic fibrosis, COVID-19, diabetes, atherosclerosis, hypertension, heart failure, myocardial infarction and repair, cardiac remodelling, cardiac arrhythmias, cardiac hypertrophy, heart failure with preserved ejection fraction, diabetic cardiomyopathy, sarcopenia, obesity, metabolic syndrome, diabetes mellitus, insulin resistance, pulmonary arterial hypertension, subarachnoid haemorrhage, intracerebral haemorrhage, ischemic stroke, beta-thalassemia, sickle cell disease, rheumatoid arthritis, irritable bowel disorder, ulcerative colitis, Crohn'
• the present invention provides the use of a compound, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of chronic obstructive pulmonary disease, asthma, pulmonary arterial hypertension, diabetes mellitus, chronic kidney disease, ulcerative colitis, Crohn's disease, inflammatory bowel disease, Friedreich's ataxia, sickle cell disease or non-alcoholic steatohepatitis.
• the present invention provides a method of treating chronic obstructive pulmonary disease, acute, chronic and severe asthma, acute lung injury/acute respiratory distress syndrome with or without accompanying multi organ dysfunction syndrome, pulmonary fibrosis including idiopathic pulmonary fibrosis, cystic fibrosis, COVID-19, diabetes, atherosclerosis, hypertension, heart failure, myocardial infarction and repair, cardiac remodelling, cardiac arrhythmias, cardiac hypertrophy, heart failure with preserved ejection fraction, diabetic cardiomyopathy, sarcopenia, obesity, metabolic syndrome, diabetes mellitus, insulin resistance, pulmonary arterial hypertension, subarachnoid haemorrhage, intracerebral haemorrhage, ischemic stroke, beta-thalassemia, sickle cell disease, rheumatoid arthritis, irritable bowel disorder, ulcerative colitis, Crohn's disease, psoriasis, radiation-induced dermatitis, atopic derma
• the present invention provides a method of treating chronic obstructive pulmonary disease, asthma, pulmonary arterial hypertension, diabetes mellitus, chronic kidney disease, ulcerative colitis, Crohn's disease, inflammatory bowel disease, Friedreich's ataxia, sickle cell disease or non-alcoholic steatohepatitis, said method comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as defined herein.
• the present invention provides a method of activating
• Nrf2 in vitro said method comprising administering an effective amount of a compound, or a pharmaceutically acceptable salt thereof.
• the present invention provides a method of activating
• Nrf2 in vivo, said method comprising administering an effective amount of a compound, or a pharmaceutically acceptable salt thereof.
• the present invention provides a method of activating
• Nrf2 in vitro and/or in vivo, said method comprising contacting a cell with an effective amount of a compound as defined herein, or a pharmaceutically acceptable salt thereof.
• the compounds of the invention may be administered alone as a monotherapy or may administered in combination with one or more additional therapeutic agents.
• the selection of the one or more additional therapeutic agents will of course vary depending on the disease or condition to be treated and its severity.
• a combination suitable for use in the treatment of a disease or condition in which Nrf2 activation is implicated comprising a compound of the invention as defined hereinbefore, or a pharmaceutically acceptable salt thereof, and another therapeutic agent.
• pulmonary fibrosis including idiopathic pulmonary fibrosis, cystic fibrosis, COVID-19, diabetes, atherosclerosis, hypertension, heart failure, myocardial infarction and repair, cardiac remodelling, cardiac arrhythmias, cardiac hypertrophy, heart failure with preserved ejection fraction, diabetic cardiomyopathy, sarcopenia, obesity, metabolic syndrome, diabetes mellitus, insulin resistance, pulmonary arterial hypertension, subarachnoid haemorrhage, intracerebral haemorrhage, ischemic stroke, beta-thalassemia, sickle cell disease, rheumatoid arthritis, irritable bowel disorder, ulcerative colitis, Crohn's disease, psoriasis, radiation-induced
• a pharmaceutical composition which comprises a compound of the invention, or a pharmaceutically acceptable salt thereof in combination with one or more additional therapeutic agents in association with a pharmaceutically acceptable diluent or carrier.
• the one or more additional therapeutic agents may comprise a further compound of the present invention. Therefore, in an embodiment, there is provided a pharmaceutical composition which comprises two compounds of the invention, or pharmaceutically acceptable salts thereof, in association with a pharmaceutically acceptable diluent or carrier.
• a combination suitable for use in the prevention or treatment of allergic disease, inflammatory disease or autoimmune disease e.g. asthma or CORD
• a cardiovascular or metabolic disease e.g. diabetes
• a neurodegenerative disease e.g. a chronic kidney or liver disease; sickle cell disease; pulmonary arterial hypertension; cancer; or for aiding transplantation.
• Examples of other therapeutic agents that may be used as part of a combination therapy with a compound of the present invention include, but are not limited to, the following:
• beta2-ad re no receptor agonists (which may be a racemate or a single enantiomer) including salmeterol, salbutamol, formoterol, salmefamol, fenoterol, carmoterol, etanterol, naminterol, clenbuterol, pirbuterol, flerbuterol, reproterol, bambuterol, indacaterol, terbutaline, vilanterol, olodaterol and salts thereof;
• anticholinergic agents that act as antagonists at the muscarinic receptors that include ipratropium (for example, as the bromide, CAS 22254-24-6, sold under the name Atrovent), oxitropium and tiotropium (for example, as the bromide, CAS 136310-93-5, sold under the name Spiriva), revatropate, LAS- 34273, Aclidinium, Glycopyrronium, Umeclidinium and salts thereof;
• ipratropium for example, as the bromide, CAS 22254-24-6, sold under the name Atrovent
• oxitropium and tiotropium for example, as the bromide, CAS 136310-93-5, sold under the name Spiriva
• revatropate LAS- 34273, Aclidinium, Glycopyrronium, Umeclidinium and salts thereof;
• Corticosteroid anti-inflammatory agents include methyl prednisolone, prednisolone, dexamethasone, fluticasone propionate, fluticasone furcate, beclomethasone esters (for example the 17-propionate ester or the 17,21 -dipropionate ester), budesonide, flunisolide, mometasone esters (for example mometasone furoate), triamcinolone acetonide, rofleponide, ciclesonide, butixocort propionate, RPR-106541, and ST-126;
• Anti-inflammatory agents including non-steroidal anti-inflammatory drugs (NSAIDs).
• NSAIDs include sodium cromoglycate, nedocromil sodium, phosphodiesterase (PDE) inhibitors (for example, theophylline, PDE4 inhibitors or mixed PDE3/PDE4 inhibitors), leukotriene antagonists, JAK inhibitors, Pi3K inhibitors, inhibitors of leukotriene synthesis (for example montelukast), iNOS inhibitors, tryptase and elastase inhibitors, beta-2 integrin antagonists and adenosine receptor agonists or antagonists (e.g.
• Vasodilator and anti-proliferative agents including Epoprostenol (Flolan), Treprostinil (Remodulin), lloprost (Ventavis), Treprostinil (Tyvaso), Bosentan (Tracleer), Ambrisentan (Letairis), Sildenafil (Revatio), Tadalafil (Ad circa);
• Anti-diabetic medications including insulins, biguanides (e.g. metformin), sulfonylureas (e.g. glimepiride), meglitinides (e.g. repaglinide), thiazolidinediones (e.g., pioglitazone), dipeptidyl peptidase IV inhibitors (e.g. sitagliptin), incretin mimetics/GLP-1 analogues (e.g. liraglutide, exenatide, dulaglutide), sodium glucose co-transporter-2 (SGLT2) inhibitors (e.g. canagliflozin, dapagliflozin and empagliflozin) and a-glucosidase inhibitors (e.g. acarbose);
• biguanides e.g. metformin
• sulfonylureas e.g. glimepiride
• meglitinides
• ASK1 inhibitors such as selonsertib, FXR agonists such as obeticholic acid, GS-9674, Px-102, ACC inhibitors such as GS-0976 and PPARa/ ⁇ agonists such as Elafibranor.
• Such conjoint/combination treatment may be achieved by way of the simultaneous, sequential or separate dosing of the individual components of the treatment.
• the individual compounds will be administered simultaneously in a combined pharmaceutical formulation.
• Such combination therapies employ the compounds of this invention within the dosage range described herein and the other pharmaceutically active agent within approved dosage ranges and/or the dosage such as described in the relevant publication reference.
• Method 1 [00161] Waters Thar PreplOO preparative SFC system (P200 CO2 pump, 2545 modifier pump, 2998 UVAZIS detector, 2767 liquid handler with Stacked Injection Module). Column: Diacel Chiralpak IA/IB/IC, YMC Amylose / Cellulose C (5 pm, 20-21 .2 x 250 mm), maintained at 40°C. Conditions: supercritical fluid CO2 and eluents chosen from MeOH, EtOH, IPA, MeCN, EtOAc, THF with modifiers chosen from Me 2 NH ⁇ , formic acid as specified. Gradient/isocratic as specified.
• reaction mixture was warmed to rt, washed with 1 M aqueous HCI (200 mL) until the washesd were pH 1 , and the organic washed with water, brine (100 mL), filtered through a phase separator and concentrated in vacuo.
• the residue was diluted with toluene (100 mL) m aLnd to this was added (1 R)-1-phenylethanamine (16.5 mL, 130 mmol; CAS 3886-69-9).
• the mixture was seeded with a few crystals of solid material from a previous batch and stirred at rt for 24 h.
• Example 1 5-(((S)-5-chloro-8-((5-(difluoromethy)-1-methyl-1 H- 1 ,2.3-triazol-4- yl)methoxy)-7-fluoro-2((1S,2R)-2-methyl-2-(1H-tetrazol-5-yl)cyclohexane-1 - carbonyl)-1.2.3.4-tetrahydroisoquinolin-1-yl)methyn-5-azaspiro[2.4]heptan-6-one
• Impure product-containing fractions were combined, concentrated in vacuo and further purified by flash column chromatography to provide additional product (1.26 g). Both batches were combined to give (1 S,2R?)-2-((S)-5-chloro-8-((5- (difluoromethyl)- 1 -methyl-1 H- 1 ,2,3-triazol-4-yl)methoxy)-7-fluoro-1 -((6-oxo-5- azaspiro[2.4]heptan-5-yl)methyl)-1 ,2,3,4-tetrahydroisoquinoline-2-carbonyl)-1- methyl cyclohexane-1 -carboxylic acid (5.66 g, 63%).
• step b in toluene (2.5 mL) was added the above intermediate (193 mg, 0.79 mmol) and triethylamine (0.13 mL, 0.910 mmol) and the reaction mixture was heated at 120°C for 18 h. The reaction mixture was cooled to rt and concentrated in vacuo. The residue was diluted with brine and extracted with DCM.
• Example 3 (1 S.2ffl-2-(fS)-5-chloro-8-(f5-fdifluoromethvh-1 -methvl-1 H- 1 ,2,3-triazol- 4-vl)methoxv)-7-fluoro-1 -(((R)-4-methyl-2-oxopvrrolidin-1 -yl)methvl)-1 ,2,3,4- tetrahvdroisoauinoline-2-carbonvl)-1 -methylcvclohexane-1 -carboxylic acid
• Step e To a stirred solution of Intermediate 8 (434 mg, 0.94 mmol) in DMF (3 ml_) was added the above intermediate (210 mg, 1.22 mmol) and cesium carbonate (920 mg, 2.82 mmol) and the reaction mixture was stirred under nitrogen at rt for 18 h. The reaction mixture was diluted with water, extracted with EtOAc and the combined organics were washed with water, brine, dried over NaaSO-t, filtered and concentrated in vacuo.
• Step h [00206] Hydrochloric acid (4M in dioxane; 2.9 ml_, 11.5 mmol) was added to the above intermediate (321 mg, 0.57 mmol) and the resulting reaction mixture left to stir at rt for 1 h.
• Step j
• Example 5 (1 S,2/?)-2-((S)-5-chloro-8-((4,5-dimethvl-4f/-1.2,4-triazol-3-v0methoxy)-7- fluoro-1 -i(6-oxo-5-azaspiror2.4lheptan-5-vl)methvl)-1,2,3,4-tetrahvdroisoauinoline-
• Example 7 (1S,2R)-2-((S)-5-chloro-7-fluoro-8- «5-methyl-1H-1 ,2,3-triazol-4- yl)methoxy)-((6-oxo-5-azaspiro[2.4]heptan-5-yl)methyl)-1,2,3,4- tetrahydroisoquinoline-2-carbonyl)-1-methylcvclohexane-1-carboxylic acid
• Each well contained 2 nM FITC-labelled NRF2 peptide (FITC-LDEETGEFL-NH2) and 25nM human KEAP1 (N- term, residues 321-609) enzyme in a final volume of 20 pL of assay buffer (50 mM Tris- HCI pH 8.0, 100 mM NaCI, 5 mM MgCL, 0.005% Tween-20, 0.005% BSA, 0.5% DMSO) in the presence of varying concentrations of test compound.
• Unlabelled peptide (LDEETGEFL-NH2) at 50 pM (negative control) and 0.5% DMSO (positive control) was used to determine the assay window.
• NRF2 mediated gene NAD(P)H:quinone acceptor oxidoreductase 1 (NQ01 ) was measured using the following assay method:
• BEAS-2B cells (ATCC CRL-9609) were plated in 96-well clear plates at 20,000 cells/well in 75 ⁇ _ of cell culture media and incubated overnight (37 °C, 5% CO2). On day 2, 25pL of compound or controls were added to the cells for 24 h. On day 3, the medium was aspirated from the plate and the Cells-to-CTTM 1-Step TaqMan® Kit (Ambion A25603) was used to perform expression analysis directly from cultured cells without RNA purification according to the manufacturer’s instructions.
• the primers/probes sets that were used for amplification of cDNA were obtained from TaqMan Gene Expression Assays (Applied Biosystems).
• the comparative CT (AACT) relative quantification method was used to calculate the relative mRNA level of the target gene NQOI as described in the Applied Biosystems Chemistry Guide.
• Test item Male Wistar Han rats (Charles River labs) were administered the test item orally or intravenously at the designated dose concentration.
• the intravenous dose was administered as a slow bolus via the tail vein.
• the oral formulation was administered by gastric gavage into the stomach. Actual dose times were recorded.
• RNAIater tissue protect tube containing 5 mL RNAIater stabilisation reagent and stored at 4°C to allow RNA stabilization (PD analysis).
• PD analysis RNA stabilization
• the remaining two sections (labelled left and right) were weighed and snap frozen by immersion in liquid nitrogen in polypropylene tubes (PK analysis).
• the liver was also collected from each animal. Six representative pieces (of a similar size to the lung pieces) from different areas (no more than 0.5 cm thickness) were collected. Four pieces (no more than 0.5 cm thickness) were placed in two separate (two pieces pertube) 5 mL RNA later tissue protect tubes containing 5 ml. RNAIater stabilisation reagent (tissue sections were completely submerged into the RNA later solution) and stored at 4°C (PD analysis). The remaining two pieces were weighed and snap frozen by immersion in liquid nitrogen in separate polypropylene tubes (a maximum of 0.5 g pertube, PK analysis).
• RNAIater RNA Stabilisation Reagent were stored at ca +4 °C to allow RNA stabilisation reagent to perfuse the tissue. Sections snap frozen were stored in a freezer set to maintain a temperature of -80°C.
• PK study samples were quantified using a method based on protein precipitation and LC-MS/MS analysis. Prior to analysis defrosted tissue samples were weighed and homogenised following the addition of HPLC grade water using an Omni- Prep Bead Ruptor (Omni Inc., Kennesaw, GA) at 4°C. Plasma and tissue homogenate samples were extracted using protein precipitation with acetonitrile acidified with 0.1% formic acid containing internal standard(s). Samples were mixed and centrifuged at 4000 rpm at 4°C for 30 minutes to remove precipitated proteins, and the supernatant diluted appropriately with HPLC grade water in a 96-well plate.
• Omni- Prep Bead Ruptor Omni- Prep Bead Ruptor
• RNA extraction and real-time PCR analysis for NQ01 gene expression in in-vivo PD studies was performed as detailed below.
• RNA was isolated using RNeasy plus mini RNA isolation kit (Qiagen) or Mouse RiboPu reTM -Blood RNA Isolation Kit (ThermoFisher Scientific) according to the manufacturer’s instructions and quantified using an Agilent RNA 6000 Nano instrument.
• Real-time PCR was performed using the C-1000 Thermal Cycler (Bio-Rad) using rat beta actin as the internal control.
• the cDNA was amplified with specific primer for NQ01/Nqo1 using universal master mix (Applied Biosystems).
• the primers/probes sets used for amplification of cDNA were obtained from TaqMan Gene Expression Assays (Applied Biosystems).
• the comparative CT ( ⁇ C ⁇ ) relative quantification method is used to calculate the relative mRNA level of the target gene NQ01 as described in the Applied Biosystems Chemistry Guide.
• the data is expressed as an increase in target gene mRNA compared to vehicle control treated for each tissue type.

Landscapes

• Health & Medical Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Animal Behavior & Ethology (AREA)
• Veterinary Medicine (AREA)
• General Health & Medical Sciences (AREA)
• Public Health (AREA)
• Life Sciences & Earth Sciences (AREA)
• Pharmacology & Pharmacy (AREA)
• Medicinal Chemistry (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• General Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Pulmonology (AREA)
• Diabetes (AREA)
• Gastroenterology & Hepatology (AREA)
• Hematology (AREA)
• Emergency Medicine (AREA)
• Obesity (AREA)
• Endocrinology (AREA)
• Urology & Nephrology (AREA)
• Biomedical Technology (AREA)
• Dermatology (AREA)
• Neurology (AREA)
• Neurosurgery (AREA)
• Cardiology (AREA)
• Heart & Thoracic Surgery (AREA)
• Epidemiology (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Plural Heterocyclic Compounds (AREA)
• Nitrogen Condensed Heterocyclic Rings (AREA)

Priority Applications (13)

Applications Claiming Priority (2)

Publications (1)

Family

ID=70860156

Family Applications (1)

Country Status (16)

Cited By (3)

Citations (3)

Family Cites Families (2)

• 2020
  • 2020-04-22 GB GBGB2005863.2A patent/GB202005863D0/en not_active Ceased
• 2021
  • 2021-04-22 US US17/920,226 patent/US20230167084A1/en active Pending
  • 2021-04-22 CN CN202180031435.0A patent/CN115667243B/zh active Active
  • 2021-04-22 AU AU2021259366A patent/AU2021259366A1/en active Pending
  • 2021-04-22 CA CA3174361A patent/CA3174361A1/en active Pending
  • 2021-04-22 EP EP21723350.1A patent/EP4139294B1/en active Active
  • 2021-04-22 KR KR1020227040550A patent/KR20230004720A/ko active Pending
  • 2021-04-22 WO PCT/GB2021/050973 patent/WO2021214470A1/en not_active Ceased
  • 2021-04-22 IL IL297434A patent/IL297434A/en unknown
  • 2021-04-22 AR ARP210101080A patent/AR121912A1/es unknown
  • 2021-04-22 PH PH1/2022/552775A patent/PH12022552775A1/en unknown
  • 2021-04-22 BR BR112022021244A patent/BR112022021244A2/pt unknown
  • 2021-04-22 MX MX2022013398A patent/MX2022013398A/es unknown
  • 2021-04-22 ES ES21723350T patent/ES3032247T3/es active Active
  • 2021-04-22 JP JP2022564519A patent/JP7679400B2/ja active Active
• 2022
  • 2022-10-19 ZA ZA2022/11465A patent/ZA202211465B/en unknown

Patent Citations (3)

Non-Patent Citations (49)

Also Published As

Similar Documents

Legal Events