WO2021179177A1 - Blood analyzer, blood analysis method, and computer readable storage medium - Google Patents

Abstract

A blood analyzer for prompting a novel coronavirus infection sample according to blood routine parameters, a blood analysis method, and a readable storage medium. The blood analyzer (100) comprises: a sampling device (110) used for sucking a blood sample to be detected; a sample preparation device (120) used for preparing a sample solution to be detected from the blood sample to be detected and a treatment reagent; a detection device (130) used for detecting the sample solution to be detected to obtain blood routine parameters; a display device (140) used for displaying information related to the blood routine parameters; and a control device (150) configured to be used for obtaining analysis parameters related to novel coronavirus infection from the blood routine parameters of the blood sample to be detected, and controlling, when the analysis parameters satisfy preset conditions, the display device (140) to display alarm information related to the novel coronavirus infection. The related information of the novel coronavirus infection can be quickly output at low cost, and an alarm is given.

Description

Blood analyzer, blood analysis method and computer readable storage medium Technical field

The embodiment of the present invention relates to the field of blood testing, and in particular, to a blood analyzer, a blood analysis method, and a computer-readable storage medium for prompting samples of novel coronavirus infection based on blood routine parameters.

Background technique

At the beginning of 2020, an infectious disease caused by the new coronavirus 2019-nCoV broke out in China, also known as the new coronavirus pneumonia (Corona Virus Disease 2019, COVID-19). The new type of coronavirus belongs to the beta genus of coronaviruses. It has an envelope, and the particles are round or elliptical, often pleomorphic, with a diameter of 60-140nm. The genetic characteristics of 2019-nCoV are significantly different from SARSr-Cov and MERSr-CoV. Current research shows that 2019-nCoV has more than 85% homology with bat SARS-like coronavirus (bat-SL-CoVZC45). When isolated and cultured in vitro, 2019-nCoV can be found in human respiratory epithelial cells in about 96 hours, while it takes about 6 days to isolate and culture in Vero E6 and Huh-7 cell lines.

In the diagnostic criteria for new coronavirus pneumonia, it is clearly stated that real-time fluorescent RT-PCR detection or viral gene sequencing is required. However, nucleic acid testing and gene sequencing take a long time, usually one day, so the suspected patient may spread the virus further due to the inability to diagnose whether the suspected patient is infected with the new coronavirus pneumonia in time.

Summary of the invention

Therefore, in view of the above situation, the purpose of the embodiments of the present invention is to provide a low-cost and rapid solution for prompting new coronavirus infection samples based on blood routine parameters, in which existing blood cell analyzers can be used in the solution. Conventional parameters provide early warning of samples of new coronavirus infection, assist medical staff in quickly determining whether a suspected patient may be infected with the new coronavirus, and even determine the degree of infection, so that medical staff can respond quickly in advance to prevent further spread of the virus. In addition, this solution does not require additional testing costs, and can realize early warning of new coronavirus infection samples while using the existing hemocytometer for routine blood testing.

The first aspect of the present invention provides a blood analyzer for prompting samples of novel coronavirus infection based on blood routine parameters. The blood cell analyzer includes: a sampling device, a pipette with a pipette nozzle, and a driving part for The pipette is driven to quantitatively suck the blood sample to be tested through the pipette nozzle; the sample preparation device has at least one reaction cell and a reagent supply part, wherein the at least one reaction cell is used to receive the sample sucked by the sampling device For a blood sample to be tested, the reagent supply part provides processing reagents to the at least one reaction cell, so that the blood sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply part react in the reaction Mixed in the pool to prepare a sample solution to be tested; a detection device for detecting the sample solution to be tested prepared by the sample preparation device to obtain blood routine parameters; a display device for displaying information related to blood routine parameters Information; and a control device including a processor and a storage medium storing a computer program, wherein the control is configured to perform the following steps when the computer program is executed by the processor: The analysis parameters related to the novel coronavirus infection are acquired from the blood routine parameters, and when the analysis parameters meet the preset conditions, the display device is controlled to display alarm information related to the novel coronavirus infection.

The second aspect of the present invention provides a blood analyzer that can be operated in a blood routine detection mode or a new coronavirus infection detection mode, including: a sampling device, a pipette with a pipette nozzle and a driving part for the driving part The pipette is driven to quantitatively suck the blood sample to be tested through the pipette nozzle; the sample preparation device has at least one reaction cell and a reagent supply part, wherein the at least one reaction cell is used to receive the sample sucked by the sampling device For the blood sample to be tested, the reagent supply part provides processing reagents to the at least one reaction cell, so that the blood sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply part are in the Mixed in the reaction tank to prepare a sample solution to be tested; a detection device for detecting the sample solution to be tested prepared by the sample preparation device to obtain blood routine parameters; a display device for displaying related blood routine parameters Mode setting device, used to set the blood routine mode or the new coronavirus infection alarm mode; and the control device, including a processor and a storage medium storing a computer program, wherein the control device is configured to When the computer program is executed by the processor, the following steps are performed:

When the new coronavirus infection detection mode is set by the mode setting device, control the sampling device, the sample preparation, and the actions of the detection device to obtain only the analysis parameters related to the new coronavirus infection,

When the analysis parameter meets the preset condition, the display device is controlled to display alarm information related to the novel coronavirus infection.

The third aspect of the present invention provides a blood analysis method for prompting new coronavirus infection samples based on blood routine parameters. The method includes the following steps: providing a blood analyzer; drawing the blood sample to be tested by the sampling and distributing device of the blood analyzer; A part of the blood sample to be tested is mixed with processing reagents in at least one reaction cell of the analyzer to prepare a sample solution to be tested; the blood cells in the sample solution to be tested are detected in the detection device of the blood analyzer to obtain Blood routine parameters; the analysis parameters related to the new coronavirus infection are obtained from the blood routine parameters of the blood sample to be tested; when the analysis parameters meet the preset conditions, the display device of the blood analyzer displays the same as the new coronavirus Information about virus infection.

The fourth aspect of the present invention provides a computer-readable storage medium applied to a blood analyzer, and a computer program is stored on the computer-readable storage medium. The computer program is characterized in that the following steps are implemented when the computer program is executed by a processor: The blood routine parameters of the blood sample to be tested are measured by the detection device of the blood analyzer; the analysis parameters related to the novel coronavirus infection are obtained from the blood routine parameters of the blood sample to be tested; when the analysis parameters meet the preset conditions At the time, it is instructed to display information related to the new coronavirus infection on the display device of the blood analyzer.

Description of the drawings

FIG. 1 is a schematic diagram of a blood analyzer provided by an embodiment of the present invention;

2 is a schematic diagram of an optical detection unit provided by an embodiment of the present invention;

FIG. 3 is a schematic diagram of an impedance detection unit provided by an embodiment of the present invention;

Figure 4 is a schematic diagram of a control device provided by an embodiment of the present invention;

Figure 5 is a schematic diagram of a general ROC curve;

FIG. 6 is an ROC curve for alerting a positive sample of new coronavirus infection by using a single blood routine parameter according to an embodiment of the present invention;

FIG. 7 is an ROC curve for alarming a positive sample of new coronavirus infection by using a linear combination of two blood routine parameters according to an embodiment of the present invention;

FIG. 8 is an ROC curve for alarming a positive sample of new coronavirus infection by using a nonlinear combination of two blood routine parameters according to an embodiment of the present invention;

FIG. 9 is an ROC curve that uses a single blood routine parameter to alarm the degree of infection of the new coronavirus according to an embodiment of the present invention;

FIG. 10 is an ROC curve that uses a linear combination of two blood routine parameters to alarm the degree of infection of the new coronavirus according to an embodiment of the present invention;

FIG. 11 is an ROC curve that uses a nonlinear combination of two blood routine parameters to alarm the degree of infection of the new coronavirus according to an embodiment of the present invention;

FIG. 12 is an ROC curve that uses a single blood routine parameter to alert the prognosis of patients infected with the new coronavirus according to an embodiment of the present invention;

FIG. 13 is an ROC curve that uses the linear combination of two blood routine parameters to alert the prognosis of patients infected with the new coronavirus according to an embodiment of the present invention;

FIG. 14 is an ROC curve that uses a linear combination of three blood routine parameters to alert the prognosis of patients infected with the new coronavirus according to an embodiment of the present invention;

FIG. 15 is an ROC curve that uses the nonlinear combination of two blood routine parameters to alert the prognosis of patients infected with the new coronavirus according to an embodiment of the present invention;

Fig. 16 is a schematic flowchart of a blood analyzer method for alarming a new coronavirus infection by using blood routine parameters according to an embodiment of the present invention.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

Throughout the specification, unless otherwise specified, the terms used herein should be understood as the meanings commonly used in the art. Therefore, unless otherwise defined, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs. If there is a conflict, this manual takes precedence.

It should be noted that in the embodiments of the present invention, the terms "including", "including" or any other variants thereof are intended to cover non-exclusive inclusion, so that a method or device including a series of elements not only includes what is clearly stated Elements, but also include other elements not explicitly listed, or elements inherent to the implementation of the method or device. Without more restrictions, the element defined by the sentence "including a..." does not exclude the existence of other related elements in the method or device that includes the element (such as steps in the method or units in the device). , The unit here can be a part of a circuit, a part of a processor, a part of a program or software, etc.).

It should be noted that the term "first\second\third" involved in the embodiment of the present invention only distinguishes similar objects, and does not represent a specific order of objects. Understandably, "first\second\third" "Three" can be interchanged in specific order or precedence when permitted. It should be understood that the objects distinguished by "first\second\third" can be interchanged under appropriate circumstances, so that the embodiments of the present invention described herein can be implemented in an order other than those illustrated or described herein.

It should be noted that in the embodiment of the present invention, ROC is an abbreviation of Receiver Operating Characteristic. ROC analysis can be used for medical decision-making evaluation, such as diagnostic radiology, laboratory medicine, cancer screening and psychiatric diagnosis, especially the evaluation of the diagnostic accuracy of medical imaging. Those skilled in the art understand that the ROC curve mainly relates to the drawing of the ROC curve, the calculation of the AUC, and the selection of the optimal operating point. The ROC curve calculates all possible thresholds, and each threshold calculates the corresponding TPR (true positive rate, that is, sensitivity, sensitivity, TPR = number of true positives/number of positives) and FPR (false positive rate, that is 1-specific Sex, 1-specificity, FPR=number of false positives/number of negatives). In this way, multiple pairs of TPR and FPR values are obtained, and then the ROC curve is drawn with FPR as the x-axis (that is, the abscissa) and TPR as the y-axis (that is, the ordinate), where the minimum value of the horizontal and vertical coordinates is 0, and the maximum value is 1. A typical ROC curve is shown in Figure 5. The black dashed line in the figure is the ROC curve. ROC curve can characterize the relationship between sensitivity and specificity, which can dynamically and objectively reflect the effectiveness of the diagnostic system. AUC is the area enclosed by the ROC curve and the x-axis (ie FPR) (Area Under the Curve). The gray area in Figure 5 is the AUC. The ideal AUC is 1, and the minimum is 0.5. The larger the AUC, the better the classification effect of the model and the more accurate the disease detection. The selection of the best operating point is to determine the best combination of TPR and FRP on the selected ROC curve. The point on the curve with the largest TPR and the smallest FPR is the best operating point. Common methods for selecting the best operating point are as follows: Positive likelihood ratio method, Youden index method (Youden index method), the method of maximizing the sum of squares of sensitivity and specificity, etc.

It is understandable that routine blood test is a method of checking the number, proportion, and morphology of various blood cells in the peripheral blood of a human by a blood cell analyzer. The blood cell analyzer is an instrument that can detect cells in the blood. It can detect white blood cells (White Blood Cell, WBC), red blood cells (RBC), platelets (PLT), and nucleated red blood cells (Nucleated Red Blood Cells). Red Blood Cell (NRBC), Reticulocyte (Ret) and other particles are counted and classified, that is, routine blood examination. Routine blood examination has diagnostic reference value, and many patients can undergo routine blood examination for auxiliary diagnosis when the cause of the disease is unknown. In addition, routine blood tests are also commonly used indicators for observing the effect of treatment, medication or discontinuation, continued treatment or discontinuation, and disease recurrence or recovery.

The blood cell analyzer can provide the following report parameters and research parameters, as shown in Table 1 and Table 2 below. It should be noted that, in the embodiment of the present invention, the white blood cell parameters are parameters related to white blood cells obtained by the blood analyzer, the red blood cell parameters are parameters related to red blood cells obtained by the blood analyzer, and the platelet parameters are obtained by the blood analyzer. The parameters related to platelets, the hemoglobin parameter is the parameter related to hemoglobin obtained by the blood analyzer.

Table 1 Description of blood test report parameters

Table 2 Description of blood test research parameters

Since the blood routine parameters of the new coronavirus infection-positive blood sample, such as white blood cell count or lymphocyte count/ratio will have specific changes compared with the negative sample, this specific change can indicate that the blood sample may be a new coronavirus infection-positive sample, or even Indicates the degree or development trend of new coronavirus infection. Based on this, the embodiment of the present invention proposes a solution for prompting samples of novel coronavirus infection based on blood routine parameters. The advantages of the solution provided by the embodiments of the present invention are fast speed and low cost, that is, blood routine testing can be completed within 15 minutes from collection to test completion at the fastest, blood routine testing charges are low, and nucleic acid testing requires a day to produce results.

The embodiment of the present invention first proposes a blood analyzer that alarms samples of new coronavirus infection based on blood routine parameters. As shown in FIG. 1, the blood analyzer 100 at least includes a sampling device 110, a sample preparation device 120, a detection device 130, a display device 140 and a control device 150.

The sampling device 110 has a pipette with a pipette nozzle (for example, a sampling needle) and has a driving part for driving the pipette to quantitatively aspirate the blood sample to be tested through the pipette nozzle, for example, the sampling needle is in the driving part. Under the driving force of the mobile phone, it moves to suck the blood sample to be tested from the sample container containing the blood sample.

The sample preparation device 120 has at least one reaction cell and a reagent supply device (not shown). The at least one reaction cell is used to receive the blood sample to be tested sucked by the sampling device 110, and the reagent supply device provides processing reagents to the at least one reaction tank, so that the blood to be tested sucked by the sampling device 110 The sample is mixed with the processing reagent provided by the reagent supply device in the reaction tank to prepare a sample solution to be tested. In some embodiments, the reagent supply device includes a first reagent supply part for supplying leukocyte reagents. The leukocyte reagents include, for example, a hemolytic agent capable of dissolving red blood cells in a blood sample and distinguishing different types of white blood cells, optionally It also includes fluorescent reagents that can stain white blood cells. In some embodiments, the reagent supply device includes a second reagent supply part for supplying red blood cell reagent, and the red blood cell reagent is, for example, a diluent. In other embodiments, the reagent supply device includes a third reagent supply part for supplying a hemoglobin reagent, for example, the hemoglobin reagent can dissolve red blood cells in a blood sample, release hemoglobin in red blood cells, and convert hemoglobin into high iron. Hemoglobin hemolytic agent. In some embodiments, the leukocyte reagent and the hemoglobin reagent are the same hemolytic agent, that is, the first reagent supply part and the third reagent supply part are the same reagent supply part.

The detection device 130 is used to detect the sample liquid to be tested prepared by the sample preparation device 120 to obtain blood routine parameters.

In some embodiments, the detection device 130 has an optical detection portion 131 for detecting a first portion prepared from a part of the blood sample to be tested and a leukocyte reagent supplied from the first reagent supply portion. The test sample fluid is tested to obtain leukocyte parameters and optionally platelet parameters. As shown in FIG. 2, the optical detection unit 131 has a light source 1311, a beam shaping assembly 1312, a flow chamber 1313 and a forward scattered light detector 1314 arranged in a straight line in sequence. On one side of the flow chamber 1313, a dichroic mirror 1316 is arranged at an angle of 45° to the straight line. Part of the lateral light emitted by the blood cells in the flow chamber 1313 passes through the dichroic mirror 1316 and is captured by the fluorescence detector 1315 arranged behind the dichroic mirror 1316 at an angle of 45° to the dichroic mirror 1316, and the other A part of the side light is reflected by the dichroic mirror 1316 and captured by the side scattered light detector 1317 arranged in front of the dichroic mirror 1316 at an angle of 45°. According to the forward scattered light signal captured by the forward scattered light detector 1314, the side scattered light signal captured by the side scattered light detector 1317, and the fluorescent signal captured by the fluorescence detector 1315, the leukocytes in the blood sample can be detected. For counting and classification, for example, white blood cells can be classified into at least neutrophils, lymphocytes, and monocytes, and optionally, platelet parameters in the blood sample can be further detected, for example, the number of platelets can be obtained.

In some embodiments, the detection device 130 includes an impedance detection part 132 for comparing a second part of the blood sample to be tested and the red blood cell reagent supplied from the second reagent supply part. The sample solution to be tested is tested to obtain red blood cell parameters and platelet parameters. For example, the impedance detection part 132 is configured as a sheath flow impedance detection part. As shown in FIG. 3, the sheath flow impedance detection part 132 includes a flow chamber 1321 having a hole 1322 with an electrode 1323. The sheath flow impedance detection unit 132 detects the DC impedance generated when the particles in the sample solution to be tested pass through the hole 1322, and outputs an electrical signal reflecting the information when the particles pass through the hole. Specifically, the sampling device 110 is driven by its driving device after the blood sample is drawn and moved to the reaction tank of the sample preparation device 120, and the drawn blood sample is injected into the reaction tank. The conveying pipeline conveys the sample solution to be tested, which has been processed by the diluent in the reaction cell, to the sheath flow impedance detection part 132, that is, to the flow chamber 1321. The sheath flow impedance detection unit 132 may also be provided with a sheath fluid tank (not shown) for supplying sheath fluid to the flow chamber 1321. In the flow chamber 1322, the sample liquid to be tested flows under the sheath liquid, and the small holes 1322 make the flow of the sample liquid to be tested into a trickle, so that the particles (formed parts) contained in the sample to be tested pass through the small holes 1322 one by one. . The electrode 1323 is electrically connected to a DC power supply 1324, and the DC power supply 1324 supplies DC power between the pair of electrodes 1323. During the period when the DC power supply 1324 provides DC power, the impedance between the pair of electrodes 1323 can be detected. The resistance signal representing the change in impedance is amplified by the amplifier 1325 and sent to the control device 150. The size of the resistance signal corresponds to the volume (size) of the particle. Therefore, the red blood cell parameters and platelet parameters of the sample solution to be tested can be obtained by signal processing on the resistance signal by the control device 150.

In some embodiments, the detection device 130 further includes a colorimetric detection unit 133, which is used to compare a part of the blood sample to be tested and the hemoglobin reagent supplied from the third reagent supply unit. The prepared third sample solution to be tested is tested to obtain hemoglobin parameters.

The display device 140 is used to display information related to blood routine parameters. For example, the display device 140 is configured as a user interface.

In some embodiments, the control device 150 includes a processor and a storage medium storing a computer program, and the control device is configured to perform the following steps when the computer program is executed by the processor: The analysis parameters related to the novel coronavirus infection are obtained from the blood routine parameters of the. When the analysis parameters meet the preset conditions, the display device 140 is controlled to display alarm information related to the novel coronavirus infection. Further, the control configuration 150 is further configured to control the display device 140 to display the blood routine parameters of the blood sample to be tested, in particular to display blood routine parameters used to obtain analysis parameters related to the novel coronavirus infection. That is, the control device 150 is configured to display the alarm information related to the novel coronavirus infection while controlling the display device 140 to display the blood routine parameters of the blood sample to be tested.

Alternatively, in other embodiments, the blood analyzer 100 may further include a mode setting device not shown, for setting the blood routine detection mode or the new coronavirus infection detection mode. Here, the control device 150 includes a processor and a storage medium storing a computer program. The control device 150 is configured to perform the following steps when the computer program is executed by the processor: In the new coronavirus infection detection mode, control the actions of the sampling device 110, the sample preparation 120 and the detection device 130 to obtain only the analysis parameters related to the new coronavirus infection; when the blood routine detection mode is set by the mode setting device , Control the actions of the sampling device 110, the sample preparation 120, and the detection device 130 to obtain blood routine parameters. That is, the blood analyzer 100 can selectively operate in the routine blood detection mode or the new coronavirus infection detection mode. In the new coronavirus infection detection mode, the blood analyzer only obtains the analysis parameters related to the new coronavirus infection; in the blood routine detection mode, the blood analyzer obtains the general blood routine parameters. Further, the control device 150 is configured to control the display device 140 to display alarm information related to the novel coronavirus infection when the analysis parameter meets the preset condition. Further, when the blood routine mode is set by the mode setting device, the control device is configured to control the display device 140 to display at least one blood routine parameter. Those skilled in the art can understand that the routine blood detection mode should be understood as the detection mode of the existing blood analyzer.

In some embodiments, as shown in FIG. 4, the control device 150 at least includes a processing component 151, a RAM 152, a ROM 153, a communication interface 154, a memory 156 and an I/O interface 155. The processing component 151, the RAM 152, the ROM 153, the communication interface 154, the memory 156, and the I/O interface 155 communicate through the bus 157. The processing component can be a CPU, GPU or other chips with computing capabilities. The memory 156 is loaded with various computer programs such as an operating system and application programs for the processor component 151 to execute, and data required to execute the computer programs. In addition, during the blood sample analysis process, any data that needs to be stored locally can be stored in the memory 156. The I/O interface 155 is composed of a serial interface such as USB, IEEE1394 or RS-232C, a parallel interface such as SCSI, IDE or IEEE1284, and an analog signal interface composed of a D/A converter and an A/D converter. An input device composed of a keyboard, a mouse, a touch screen or other control buttons is connected to the I/O interface 155, and the user can use the input device to directly input data to the control device 150. In addition, the I/O interface 155 can also be connected to a display device 140 with a display function, such as a liquid crystal screen, a touch screen, an LED display screen, etc. The control device 150 can output the processed data as image display data to the display device 140 for display, such as analysis data, instrument operating parameters, and so on. The communication interface 154 is an interface that can be any currently known communication protocol. The communication interface 154 communicates with the outside world through the network. The control device 150 can transmit data to any device connected through the network through the communication interface 154 in a certain communication protocol.

In some embodiments, as shown in FIG. 1, the blood analyzer 100 further includes a first housing 160 and a second housing 170. The detection device 130 and the control device 150 are arranged inside the second housing 170 and are respectively arranged on both sides of the second housing 170. The sample preparation device 120 is provided inside the first housing 160. The display device 140 is disposed on the outer surface of the first cabinet 160.

In some embodiments, the blood analyzer 100 further includes a blood sample distribution device (not shown), which is used to divide the blood sample to be tested drawn by the sampling device 110 into at least two different blood samples to It is used to prepare the sample solution for testing different blood routine parameters. For example, the blood sample distribution device is used to divide the blood sample to be tested into a first part of blood sample and a second part of blood sample. The second sample solution to be tested for red blood cell detection is prepared from the second part of blood sample and red blood cell reagent, and the third sample solution to be tested for hemoglobin detection is optionally composed of the first part of blood sample or the second part of blood sample and hemoglobin. Reagents are prepared. Alternatively, the blood sample distribution device is used to divide the blood sample to be tested into a first part of blood sample, a second part of blood sample, and a third part of blood sample, and the first part of blood sample to be tested is used for leukocyte detection. The second test sample solution for red blood cell detection is prepared from the second part of blood sample and red blood cell reagent, and the third test sample solution for hemoglobin test is prepared from the third part of blood sample and hemoglobin. Reagents are prepared. The blood sample dispensing device can be configured as a blood separation valve, for example. Optionally, the blood sample distribution device may also be the sampling needle of the sampling device 110. In this case, the driving part of the sampling device 110 drives the sampling needle to move to the positions of the different reaction cells of the sample preparation device 120 to separately A part of the blood sample is allocated to different reaction tanks to react with corresponding processing reagents.

In some embodiments, the analysis parameters include at least two blood routine parameters, or a combined parameter including at least two blood routine parameters, such as linear or non-linear combined parameters. In these embodiments, when at least two blood routine parameters meet corresponding predetermined conditions, or a parameter combined by at least two blood routine parameters meets a predetermined condition, the control device 150 controls the display device 140 to display that the virus is infected with the new coronavirus. Related alarm information.

In some embodiments, the analysis parameters include at least one of white blood cell parameters, red blood cell parameters, platelet parameters, and hemoglobin parameters. For example, the analysis parameters include at least one white blood cell parameter or at least one red blood cell parameter or at least one platelet parameter or at least one platelet parameter. A hemoglobin parameter.

In some embodiments, the analysis parameter includes a combination of a plurality of parameters selected from white blood cell parameters, red blood cell parameters, platelet parameters, and hemoglobin parameters. For example, the analysis parameter includes a combination of multiple white blood cell parameters or a combination of multiple red blood cell parameters or a combination of multiple platelet parameters. For example, the analysis parameter includes a combination of at least one white blood cell parameter and at least one red blood cell parameter, or a combination of at least one white blood cell parameter and at least one platelet parameter, or a combination of at least one red blood cell parameter and at least one platelet parameter, or at least one white blood cell parameter, or at least one white blood cell parameter. A combination of red blood cell parameters and at least one platelet parameter.

Further, the analysis parameter includes at least one blood routine parameter among the following blood routine parameters or a combination of multiple blood routine parameters in the following blood routine parameters, such as linear or non-linear combination parameters: white blood cell count, number of neutrophils , Percentage of neutrophils, number of lymphocytes, percentage of lymphocytes, ratio of neutrophils to lymphocytes, ratio of platelets to lymphocytes, coefficient of variation of red blood cell distribution width, standard deviation of red blood cell distribution width, number of red blood cells, proportion of large red blood cells, small Red blood cell ratio, standard deviation of platelet distribution width, average platelet volume, large platelet ratio, percentage of cells with high fluorescence intensity, average red blood cell hemoglobin concentration, hemoglobin concentration.

Preferably, the analysis parameters include at least one blood routine parameter among the following blood routine parameters and a combination of multiple blood routine parameters from the following blood routine parameters: neutrophil to lymphocyte ratio, lymphocyte percentage, red blood cell distribution width Coefficient of variation, standard deviation of red blood cell distribution width, percentage of cells with high fluorescence intensity.

In the process of studying the blood routine parameters of a large number of new coronavirus infection samples, the inventor found that the parameters related to the distribution width of red blood cells have a better alarm effect for alarming new coronavirus infection samples. Therefore, in some embodiments, the analysis parameter includes at least one red blood cell distribution width parameter, in particular, includes the red blood cell distribution width coefficient of variation or the red blood cell distribution width standard deviation, or includes the red blood cell distribution width coefficient of variation and the red blood cell distribution width standard deviation. In some embodiments, the analysis parameter includes a combination parameter of at least one red blood cell distribution width parameter and at least one additional blood routine parameter, for example, the at least one additional blood routine parameter includes a ratio of neutrophils to lymphocytes, lymphocytes Percent or percentage of cells with high fluorescence intensity. In this way, it is possible to use the red blood cell distribution width parameter to alert the samples of the novel coronavirus infection.

In the process of studying the blood routine parameters of a large number of new coronavirus infection samples, the inventor found that it is particularly advantageous to combine the ratio of neutrophils to lymphocytes and other blood routine parameters to alarm samples for new coronavirus infections. Therefore, preferably, the analysis parameter includes a combined parameter of the ratio of neutrophils to lymphocytes and at least one additional blood routine parameter. The at least one additional blood routine parameter includes, for example, at least one red blood cell parameter, in particular a red blood cell distribution width parameter (for example, the coefficient of variation of the red blood cell distribution width or the standard deviation of the red blood cell distribution width).

In some embodiments, the alarm information related to the novel coronavirus infection includes alarm information for prompting that the blood sample to be tested is a positive sample for the novel coronavirus infection. For example, when the analysis parameter satisfies the first preset condition, an alarm message for prompting that the blood sample to be tested is a positive sample of novel coronavirus infection is displayed on the display device of the blood analyzer. In these embodiments, the blood sample to be tested may be a blood sample of a patient suspected of being infected with the novel coronavirus.

In the case where the blood analyzer provided by the embodiment of the present invention is used to output alarm information prompting that the blood sample to be tested is a positive sample of novel coronavirus infection, it is particularly advantageous that the analysis parameters include the following blood routine parameters At least one blood routine parameter or a combination of multiple blood routine parameters from the following blood routine parameters, such as linear or non-linear combination parameters: white blood cell count, lymphocyte percentage, neutrophil to lymphocyte ratio, red blood cell distribution width parameter (e.g. Coefficient of variation of red blood cell distribution width). Of course, the analysis parameter may also include at least one parameter among the above blood routine parameters and a combination of multiple parameters among the above blood routine parameters. Preferably, the analysis parameter includes a red blood cell distribution width parameter, especially a coefficient of variation of the red blood cell distribution width. In some embodiments, the analysis parameters may also include a combination parameter of the ratio of neutrophils to lymphocytes and the red blood cell distribution width parameter, especially the coefficient of variation of the red blood cell distribution width.

As shown in Figures 6 to 8, the inventors studied the use of one parameter or multiple parameter combinations in WBC, lymphocyte percentage LYM%, neutrophil to lymphocyte ratio NLR, and red blood cell distribution width variation coefficient RDW_CV. Cases of blood samples are effective for positive alarms for new coronavirus infections, of which 156 are positive samples for new coronavirus infections and 162 are negative samples. Figure 6 shows the ROC curves of WBC, lymphocyte percentage LYM%, neutrophil to lymphocyte ratio NLR, and red blood cell distribution width variation coefficient RDW_CV for positive alarm of new coronavirus infection, as can be seen from Figure 6, used alone The alarm effect of the coefficient of variation of red blood cell distribution width RDW_CV is AUC=0.78, the specificity is 63%, the sensitivity is 78.8%, and the effect is better. Figure 7 is the ROC curve of the positive alarm of new coronavirus infection using the linear combination of WBC and LYM% and the linear combination of NLR and RDW_CV. As can be seen from Figure 7, the linear combination parameters of NLR and RDW_CV (the linear combination formula is, for example, 0.0585*NLR -0.352*RDW_CV+4.6641) AUC can reach 0.83 (specificity is 69.1%, sensitivity is 80.1%), which is significantly better than the parameter combination of WBC and LYM% (AUC is 0.71, specificity is 67.3%, sensitivity is 63.5% ). Figure 8 is the ROC curve of the positive alarm of new coronavirus infection using the nonlinear combination of NLR and RDW_CV. The AUC is 0.82, the specificity is 71.6%, and the sensitivity is 72.4%. The nonlinear combination formula of NLR and RDW_CV is, for example:

-0.3332*NLR+46.1415*RDW_CV+0.0186NLR.^2+0.0165*NLR*RDW_CV-1.8844*RDW_CV.^2-281.615.

In the embodiment of the present invention, the method of linear discriminant analysis (LDA) is selected for linear combination analysis, and the method of QDA (quadratic discriminant analysis) is selected for nonlinear combination analysis.

Due to the different degree of infection, some blood routine parameters of patients with new coronary pneumonia have different numerical characteristics. For example, in the early stage of the disease, the total number of white blood cells will be normal or decreased, and the lymphocyte count will decrease. Therefore, in some embodiments, the alarm information related to the novel coronavirus infection includes alarm information for prompting the degree of the novel coronavirus infection of the blood sample to be tested. For example, when the analysis parameter satisfies the second preset condition, the display device of the blood analyzer displays alarm information for prompting the degree of new coronavirus infection of the blood sample to be tested. The degree of new coronavirus infection includes mild and severe. That is to say, the blood analyzer provided by the embodiment of the present invention can not only alarm whether the blood sample is a positive sample of the novel coronavirus, but also can further alarm the infection degree of the positive sample of the novel coronavirus. In this case, the blood sample to be tested is preferably a blood sample of a patient infected with the novel coronavirus.

Advantageously, the analysis parameters include at least one blood routine parameter among the following blood routine parameters or a combination parameter of multiple blood routine parameters in the following blood routine parameters, such as linear or non-linear combination parameters: white blood cell count, lymphocyte Percentage, percentage of neutrophils, ratio of platelets to lymphocytes, parameters of red blood cell distribution width (such as the standard deviation of red blood cell distribution width), and the number of red blood cells. Of course, the analysis parameter may also include at least one parameter among the above blood routine parameters and a combination of multiple parameters among the above blood routine parameters. Preferably, the analysis parameter includes the percentage of lymphocytes. Further preferably, the analysis parameters include a combination parameter of lymphocyte percentage and red blood cell distribution width parameter, especially a combination parameter of red blood cell distribution width standard deviation, especially a linear combination parameter. In some embodiments, the analysis parameters may also include combined parameters of the ratio of neutrophils to lymphocytes and the number of red blood cells, especially non-linear combined parameters.

As shown in Figures 9 to 11, the inventors studied the use of white blood cell count WBC, lymphocyte percentage LYM%, neutrophil percentage Neu%, platelet to lymphocyte ratio PLR, red blood cell distribution width standard deviation RDW_SD, red blood cell number RBC The effectiveness of one parameter or a combination of multiple parameters to alert 239 positive samples of new coronavirus infection, of which 108 were severe samples and 131 were mild samples. Figure 9 shows the ROC curves of WBC, lymphocyte percentage LYM%, platelet-to-lymphocyte ratio PLR, and red blood cell distribution width standard deviation RDW_SD for alarming the degree of new coronavirus infection. Figure 9 shows that lymphocyte percentage is used alone. The alarm effect of LYM% is AUC=0.84, and the effect is better. Fig. 10 is the ROC curve of the new coronavirus infection degree alarm using the linear combination of LYM% and RDW_SD, where AUC=0.9, and the linear combination formula of LYM% and RDW_SD is, for example, -0.0857*Lym%+0.1337*RDW_SD-3.5294. Figure 11 is the ROC curve of the new coronavirus infection level alarm using the nonlinear combination of Neu% and RBC, where AUC=0.85, and the nonlinear combination formula of Neu% and RBC is, for example:

0.5705*Neu%-9.1627*RBC-0.0037*Neu%.^2+0.039*Neu%*RBC+0.6265*RBC.^2-7.9552.

In some embodiments, the alarm information related to the novel coronavirus infection includes alarm information for prompting the development trend of the novel coronavirus infection of the blood sample to be tested. For example, when the analysis parameter satisfies the third preset condition, the display device of the blood analyzer displays alarm information for prompting the development trend of the novel coronavirus infection of the blood sample to be tested. In other words, the blood sample analyzer provided by the embodiment of the present invention can also assist medical staff in predicting the course of the disease of a confirmed patient with the new coronavirus, that is, it can alert the prognosis of the new coronavirus infection. In the embodiments of the present invention, a poor prognosis means that due to a critical illness, or there is no effective treatment method, the diseased animal may die or cannot be completely cured, which affects production performance or economic value; a good prognosis means that the disease is mild and the diseased animal Individuals are in good condition and can be cured with sufficient evidence, which not only restores health, but also does not affect production performance and economic value. In this case, the blood sample to be tested is preferably a blood sample of a patient infected with the novel coronavirus.

In the case where the blood analyzer provided by the embodiment of the present invention is used to alarm the prognosis of the novel coronavirus infection, it is particularly advantageous that the analysis parameter includes at least one of the following blood routine parameters or the following blood routine parameters Combination parameters of multiple blood routine parameters in: red blood cell distribution width parameters (such as red blood cell distribution width standard deviation), large red blood cell ratio, small red blood cell ratio, platelet to lymphocyte ratio, platelet distribution width parameters (such as platelet distribution width and platelet distribution Width standard deviation), large platelet ratio, average platelet volume, high fluorescence intensity cell percentage, average red blood cell hemoglobin concentration. Preferably, the analysis parameter includes a large platelet ratio. In some embodiments, the analysis parameters include a combination parameter of a high fluorescence intensity cell percentage and a red blood cell distribution width parameter, especially a combination parameter of a red blood cell distribution width standard deviation, especially a linear combination parameter. In some embodiments, the analysis parameters include combined parameters of the proportion of large red blood cells, average platelet volume, and average red blood cell hemoglobin concentration, especially linear combined parameters. In still other embodiments, the analysis parameter includes a combination parameter of the proportion of small red blood cells and a parameter of platelet distribution width, especially a combination parameter of the standard deviation of platelet distribution width, especially a non-linear combination parameter.

As shown in Figures 12 to 15, the inventors studied the use of the standard deviation of the red blood cell distribution width RDW_SD, the proportion of macro red blood cells MacroRBC%, the proportion of small red blood cells MicroRBC%, the ratio of platelets to lymphocytes PLR, the platelet distribution width PDW, and the standard deviation of platelet distribution width PDW_SD The effectiveness of one parameter or a combination of multiple parameters in the large platelet ratio P_LCR, average platelet volume MPV, high fluorescence intensity cell percentage HFC%, and average red blood cell hemoglobin concentration MCHC for prognostic warning of 52 cases of positive samples of new coronavirus infection, among which, There were 23 samples with poor prognosis and 29 samples with good prognosis. Figure 12 shows the ROC curve for prognostic warning of novel coronavirus infection using the platelet-to-lymphocyte ratio PLR, platelet distribution width PDW, large platelet ratio P_LCR, and high fluorescence intensity cell percentage HFC% respectively. It can be seen from Figure 12 that large platelets are used alone The alarm effect of the ratio P_LCR is AUC=0.83, which is better. Fig. 13 is the ROC curve of using the linear combination of HFC% and RDW_SD for prognostic warning of novel coronavirus infection, where AUC=0.83, and the combination formula of HFC% and RDW_SD is, for example, 2.2388*HFC%+0.4138*RDW_SD-6.1972. Figure 14 is the ROC curve of using the linear combination of MCHC, MacroRBC% and MPV for prognostic warning of new coronavirus infection, where AUC=0.86, and the combined formula of MCHC, MacroRBC% and MPV is, for example, -0.0374*MCHC-0.4008*MacroRBC% +0.8149*MPV+5.8068. Figure 15 is the ROC curve of using the non-linear combination of MicroRBC% and PDW_SD for prognostic warning of novel coronavirus infection, where AUC=0.85, and the non-linear combination formula of MicroRBC% and PDW_SD is, for example:

0.1303*MicroRBC%-4.69*PDW_SD-0.2429*MicroRBC%.^2+0.1432*MicroRBC%*PDW_SD+0.2166*PDW_SD.^2+22.3023.

The embodiment of the present invention also provides a blood analysis method for alarming samples of new coronavirus infection according to blood routine parameters. As shown in FIG. 16, the blood analysis method 200 includes the following steps:

S210, provide blood analyzer;

S220, the blood sample to be tested is sucked by the sampling and distribution device of the blood analyzer;

S230, mixing a part of the blood sample to be tested and the processing reagent in at least one reaction cell of the blood analyzer to prepare a sample liquid to be tested;

S240, detecting blood cells in the sample solution to be tested in the detection device of the blood analyzer to obtain blood routine parameters;

S250: Obtain analysis parameters related to the novel coronavirus infection from the blood routine parameters of the blood sample to be tested;

S260: When the analysis parameter meets the preset condition, display information related to the novel coronavirus infection on the display device of the blood analyzer.

In some embodiments, after the blood sample aspiration step S220 and before the preparation of the sample liquid to be tested step S230, the method further includes: the sampling and distributing device divides the blood sample to be tested into at least two parts. For example, the sampling and distribution device divides the blood sample to be tested into a first part of blood sample, a second part of blood sample, and optionally a third part of blood sample.

Here, the step S230 of preparing a sample solution to be tested includes: mixing the first part of blood sample and white blood cell reagent to prepare a first sample solution to be tested for detecting white blood cell parameters; mixing the second part of blood sample and red blood cell reagent , To prepare a second sample solution to be tested for detecting red blood cell parameters; mixing the first part of blood sample, the second part of blood sample or the third part of blood sample with a hemoglobin reagent to prepare a reagent for detecting hemoglobin parameters The third sample solution to be tested. That is, the same part of the blood sample can be used to prepare the third sample solution to be tested and the first sample solution to be tested, or the same part of blood sample can be used to prepare the third sample solution to be tested and the second sample solution to be tested, or Different parts of blood samples are respectively used to prepare the first, second and third sample liquids to be tested.

Here, the blood routine parameter detection step S240 includes: detecting the first sample liquid to be tested in the optical detection part of the blood analyzer to obtain white blood cell parameters, and optionally platelet parameters; The impedance detection unit detects the second sample liquid to be tested to obtain red blood cell parameters and platelet parameters; and detects the third sample liquid to be tested in the colorimetric detection unit of the blood analyzer to obtain hemoglobin parameters.

In some embodiments, the step of obtaining the white blood cell parameters includes: counting and classifying the white blood cells in the first sample solution to be tested in the optical detection part of the blood analyzer. For example, the white blood cell parameters include at least the white blood cell count, the number and ratio of neutrophils, the number and ratio of lymphocytes, and the number and ratio of monocytes.

For other embodiments of the blood analysis method 200 provided in the present invention, reference may be made to the above description of the blood analyzer 100 provided in the embodiments of the present invention, and details are not described herein again.

The embodiment of the present invention also provides a computer-readable storage medium applied to a blood analyzer, the computer-readable storage medium stores a computer program, and is characterized in that, when the computer program is executed by a processor, the following steps are implemented: The blood routine parameters of the blood sample to be tested are measured by the detection device of the blood analyzer; the analysis parameters related to the novel coronavirus infection are obtained from the blood routine parameters of the blood sample to be tested; when the analysis parameters meet the preset conditions At the time, it is instructed to display information related to the new coronavirus infection on the display device of the blood analyzer.

For other embodiments of the computer-readable storage medium provided by the present invention, reference may be made to the above description of the blood analyzer 100 and the blood analysis method 200 provided by the embodiments of the present invention, and details are not described herein again.

Each individual feature or combination of features described in each embodiment of the specification, drawings and claims can be combined with each other arbitrarily as long as they are meaningful within the scope of the embodiments of the present invention and do not contradict each other. The details, features, and advantages described for the blood analyzer 100 provided by the embodiment of the present invention are applicable to the blood analysis method 200 and the computer-readable storage medium provided by the embodiment of the present invention in a corresponding manner, and vice versa.

The above are only specific embodiments of the present invention, so that those skilled in the art can understand or implement the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown in this document, but should conform to the widest scope consistent with the principles and novel features applied for in this document.

Claims (31)

1. A blood analyzer, characterized in that it comprises:

   A sampling device having a pipette with a pipette nozzle and a driving part for driving the pipette to quantitatively suck the blood sample to be tested through the pipette nozzle;

   The sample preparation device has at least one reaction cell and a reagent supply part, wherein the at least one reaction cell is used to receive the blood sample to be tested sucked by the sampling device, and the reagent supply part provides processing reagents to the at least one A reaction tank, so that the blood sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply part are mixed in the reaction tank to prepare a sample solution to be tested;

   A detection device for detecting the sample liquid to be tested prepared by the sample preparation device to obtain blood routine parameters;

   A display device for displaying information related to blood routine parameters; and

   The control device includes a processor and a storage medium storing a computer program, wherein the control device is configured to perform the following steps when the computer program is executed by the processor:

   Obtain analysis parameters related to the novel coronavirus infection from the blood routine parameters of the blood sample to be tested,

   When the analysis parameter meets the preset condition, the display device is controlled to display alarm information related to the novel coronavirus infection.
2. A blood analyzer, characterized in that it comprises:

   A sampling device having a pipette with a pipette nozzle and a driving part for driving the pipette to quantitatively suck the blood sample to be tested through the pipette nozzle;

   The sample preparation device has at least one reaction cell and a reagent supply part, wherein the at least one reaction cell is used to receive the blood sample to be tested sucked by the sampling device, and the reagent supply part provides processing reagents to the at least one A reaction tank, so that the blood sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply part are mixed in the reaction tank to prepare a sample solution to be tested;

   A detection device for detecting the sample liquid to be tested prepared by the sample preparation device to obtain blood routine parameters;

   Display device for displaying information related to blood routine parameters;

   Mode setting device for setting blood routine test mode or new coronavirus infection detection mode; and

   The control device includes a processor and a storage medium storing a computer program, wherein the control device is configured to perform the following steps when the computer program is executed by the processor:

   When the new coronavirus infection detection mode is set by the mode setting device, control the sampling device, the sample preparation, and the actions of the detection device to obtain only the analysis parameters related to the new coronavirus infection,

   When the analysis parameter meets the preset condition, the display device is controlled to display alarm information related to the novel coronavirus infection.
3. The blood analyzer according to claim 1 or 2, wherein the analysis parameter includes at least two blood routine parameters, especially a combination parameter of at least two blood routine parameters, such as a linear or non-linear combination parameter.
4. The blood analyzer according to any one of claims 1 to 3, wherein the reagent supply unit includes a first reagent supply unit for supplying white blood cell reagent, and a second reagent supply unit for supplying red blood cell reagent. And a third reagent supply part for supplying hemoglobin reagent;

   The detection device includes:

   An optical detection unit for detecting a first sample solution prepared from a part of the blood sample to be tested and a leukocyte reagent supplied from the first reagent supply unit to obtain leukocyte parameters and Selectively obtain platelet parameters,

   Impedance detection part, which is used to detect the second test sample liquid prepared from a part of the blood sample to be tested and the red blood cell reagent supplied from the second reagent supply part to obtain red blood cell parameters and platelets parameter,

   A colorimetric detection unit for detecting a third sample solution prepared by a part of the blood sample to be tested and a hemoglobin reagent supplied from the third reagent supply unit to obtain Hemoglobin parameters;

   Wherein, the analysis parameters include at least one of white blood cell parameters, red blood cell parameters, platelet parameters, and hemoglobin parameters, or the analysis parameters include a combination of multiple parameters selected from white blood cell parameters, red blood cell parameters, platelet parameters, and hemoglobin parameters parameter.
5. The blood analyzer according to any one of claims 1 to 4, wherein the analysis parameters include at least one blood routine parameter among the following blood routine parameters and/or multiple blood routine parameters from the following blood routine parameters The combination of parameters: white blood cell count, neutrophil percentage, lymphocyte percentage, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, coefficient of variation of red blood cell distribution width, standard deviation of red blood cell distribution width, number of red blood cells, large red blood cells Proportion, small red blood cell ratio, standard deviation of platelet distribution width, average platelet volume, large platelet ratio, high fluorescence intensity cell percentage, average red blood cell hemoglobin concentration;

   Preferably, the analysis parameters include at least one of the following blood routine parameters and/or a combination of multiple blood routine parameters among the following blood routine parameters: neutrophil to lymphocyte ratio, lymphocyte percentage, Coefficient of variation of red blood cell distribution width, standard deviation of red blood cell distribution width, percentage of cells with high fluorescence intensity.
6. The blood analyzer according to any one of claims 1 to 5, wherein the analysis parameter includes at least one red blood cell distribution width parameter or the analysis parameter includes at least one red blood cell distribution width parameter and at least one additional blood cell distribution width parameter. Combination parameters of conventional parameters;

   Preferably, the red blood cell distribution width parameter includes the coefficient of variation of the red blood cell distribution width and the standard deviation of the red blood cell distribution width.
7. The blood analyzer according to any one of claims 1 to 6, wherein the alarm information related to the novel coronavirus infection includes information for prompting that the blood sample to be tested is a positive sample for the novel coronavirus infection. Alarm information.
8. The blood analyzer according to claim 7, wherein the analysis parameter comprises at least one blood routine parameter among the following blood routine parameters and/or a combination parameter of a plurality of blood routine parameters from the following blood routine parameters: white blood cells Count, percentage of lymphocytes, ratio of neutrophils to lymphocytes, coefficient of variation of red blood cell distribution width.
9. The blood analyzer according to claim 8, wherein the analysis parameter comprises a coefficient of variation of red blood cell distribution width; and/or

   The analysis parameters include combined parameters of the ratio of neutrophils to lymphocytes and the red blood cell distribution width and the coefficient of variation of the red blood cell distribution width.
10. The blood analyzer according to any one of claims 1 to 9, wherein the alarm information related to the novel coronavirus infection includes an alarm for prompting the degree of the novel coronavirus infection of the blood sample to be tested information.
11. The blood analyzer according to claim 10, wherein the analysis parameters include at least one blood routine parameter among the following blood routine parameters and/or a combined parameter of multiple blood routine parameters among the following blood routine parameters: white blood cells Count, percentage of lymphocytes, percentage of neutrophils, ratio of platelets to lymphocytes, standard deviation of red blood cell distribution width, number of red blood cells.
12. The blood analyzer according to claim 11, wherein the analysis parameter includes the percentage of lymphocytes; and/or

    The analysis parameters include a combination parameter of the percentage of lymphocytes and the standard deviation of the red blood cell distribution width, especially a linear combination parameter; and/or

    The analysis parameter includes a combination parameter of the percentage of neutrophils and the number of red blood cells, especially a non-linear combination parameter.
13. The blood analyzer according to any one of claims 1 to 12, wherein the alarm information related to the novel coronavirus infection includes information for prompting the development trend of the novel coronavirus infection of the blood sample to be tested. Alarm information.
14. The blood analyzer according to claim 13, wherein the analysis parameter comprises at least one blood routine parameter among the following blood routine parameters and/or a combination parameter of a plurality of blood routine parameters from the following blood routine parameters: red blood cells The standard deviation of the distribution width, the ratio of large red blood cells, the ratio of small red blood cells, the ratio of platelets to lymphocytes, the distribution width of platelets, the standard deviation of platelet distribution width, the ratio of large platelets, the average platelet volume, the percentage of cells with high fluorescence intensity, the average red blood cell hemoglobin concentration.
15. The blood analyzer according to claim 14, wherein the analysis parameter includes a large platelet ratio; and/or

    The analysis parameters include a combination parameter of a high fluorescence intensity cell percentage and a standard deviation of the red blood cell distribution width, especially a linear combination parameter; and/or

    The analysis parameters include combined parameters of the proportion of large red blood cells, average platelet volume, and average red blood cell hemoglobin concentration, especially linear combined parameters; and/or

    The analysis parameters include a combination parameter of the proportion of small red blood cells and the standard deviation of the platelet distribution width, especially a non-linear combination parameter.
16. The blood analyzer according to any one of claims 10 to 15, wherein the blood sample to be tested is derived from a patient infected with a novel coronavirus.
17. The blood analyzer according to any one of claims 1 to 16, wherein the control is configured to perform the following steps when the computer program is executed by the processor:

    The display device is controlled to display the blood routine parameters of the blood sample to be tested, especially the blood routine parameters used to obtain the analysis parameters related to the novel coronavirus infection.
18. A blood analysis method, including:

    Provide blood analyzer;

    The blood sample to be tested is sucked by the sampling and distribution device of the blood analyzer;

    Mixing a part of the blood sample to be tested with processing reagents in at least one reaction cell of the blood analyzer to prepare a sample liquid to be tested;

    Detecting blood cells in the sample solution to be tested in the detection device of the blood analyzer to obtain blood routine parameters;

    Obtain analysis parameters related to the novel coronavirus infection from the blood routine parameters of the blood sample to be tested;

    When the analysis parameters meet the preset conditions, the information related to the novel coronavirus infection is displayed on the display device of the blood analyzer.
19. The method according to claim 18, wherein the analysis parameter includes at least two blood routine parameters, especially a combination parameter of at least two blood routine parameters, such as a linear or non-linear combination parameter.
20. The method according to claim 18 or 19, wherein after the blood sample to be tested is sucked by the sampling and distribution device of the blood analyzer, the method further comprises: the sampling and distribution device divides the blood sample to be tested Be at least two parts;

    Wherein, the steps of preparing the sample solution to be tested include:

    Mixing the first part of the blood sample to be tested with the leukocyte reagent to prepare a first sample solution to be tested,

    Mixing the second part of the blood sample to be tested with the red blood cell reagent to prepare a second sample solution to be tested,

    Mixing a part of the blood sample to be tested with the hemoglobin reagent to prepare a third sample solution to be tested;

    Among them, the blood routine parameter detection steps include:

    Detecting the first sample liquid to be tested in the optical detection part of the blood analyzer to obtain leukocyte parameters and optionally platelet parameters,

    The second sample solution to be tested is detected in the impedance detection part of the blood analyzer to obtain red blood cell parameters and platelet parameters,

    Detecting the third sample solution to be tested in the colorimetric detection part of the blood analyzer to obtain hemoglobin parameters;

    Wherein, the analysis parameters include at least one of white blood cell parameters, red blood cell parameters, platelet parameters, and hemoglobin parameters, or the analysis parameters include a combination of multiple parameters selected from white blood cell parameters, red blood cell parameters, platelet parameters, and hemoglobin parameters parameter.
21. The method according to claim 20, wherein the step of obtaining white blood cell parameters comprises:

    Counting and classifying the white blood cells in the first sample solution to be tested in the optical detection part of the blood analyzer;

    Wherein, the white blood cell parameters include at least the white blood cell count, the number and ratio of neutrophils, the number and ratio of lymphocytes, and the number and ratio of monocytes.
22. The method according to any one of claims 18 to 21, wherein the analysis parameters include at least one of the following blood routine parameters and/or a number of blood routine parameters in the following blood routine parameters Combination parameters: ratio of neutrophils to lymphocytes, percentage of lymphocytes, coefficient of variation of red blood cell distribution width, standard deviation of red blood cell distribution width, percentage of cells with high fluorescence intensity.
23. The method according to any one of claims 18 to 21, wherein the analysis parameter comprises at least one red blood cell distribution width parameter or the analysis parameter comprises at least one red blood cell distribution width parameter and at least one additional blood routine parameter The combination of parameters;

    Preferably, the red blood cell distribution width parameter includes the coefficient of variation of the red blood cell distribution width and the standard deviation of the red blood cell distribution width.
24. The method according to any one of claims 18 to 23, wherein the alarming step comprises:

    When the analysis parameter satisfies the first preset condition, an alarm message for prompting that the blood sample to be tested is a positive sample of novel coronavirus infection is displayed on the display device of the blood analyzer.
25. The method according to claim 24, wherein the analysis parameters include at least one of the following blood routine parameters and/or a combination of multiple blood routine parameters among the following blood routine parameters: white blood cell count, Percentage of lymphocytes, ratio of neutrophils to lymphocytes, coefficient of variation of red blood cell distribution width;

    Preferably, the analysis parameter includes a coefficient of variation of the distribution width of red blood cells, and/or the analysis parameter includes a combined parameter of the ratio of neutrophils to lymphocytes and the coefficient of variation of the distribution width of red blood cells.
26. The method according to any one of claims 18 to 25, wherein the alarming step comprises:

    When the analysis parameter satisfies the second preset condition, the display device of the blood analyzer displays alarm information for prompting the new coronavirus infection degree of the blood sample to be tested.
27. The method according to claim 26, wherein the analysis parameters include at least one of the following blood routine parameters and/or a combination of multiple blood routine parameters among the following blood routine parameters: white blood cell count, Percentage of lymphocytes, percentage of neutrophils, ratio of platelets to lymphocytes, standard deviation of red blood cell distribution width, number of red blood cells;

    Preferably, the analysis parameter includes the percentage of lymphocytes, and/or the analysis parameter includes a combination parameter of the percentage of lymphocytes and the standard deviation of the red blood cell distribution width, especially a linear combination parameter, and/or the analysis parameter includes neutrophils. A combination parameter of cell percentage and number of red blood cells, especially a non-linear combination parameter.
28. The method according to any one of claims 18 to 27, wherein the alarming step comprises:

    When the analysis parameter satisfies the third preset condition, the display device of the blood analyzer displays alarm information for prompting the development trend of the novel coronavirus infection of the blood sample to be tested.
29. The method according to claim 28, wherein the analysis parameter comprises at least one blood routine parameter among the following blood routine parameters and/or a combination parameter of a plurality of blood routine parameters from the following blood routine parameters: red blood cell distribution width Standard deviation, ratio of large red blood cells, ratio of small red blood cells, ratio of platelets to lymphocytes, platelet distribution width, standard deviation of platelet distribution width, large platelet ratio, average platelet volume, high fluorescence intensity cell percentage, average red blood cell hemoglobin concentration;

    Preferably, the analysis parameter includes a large platelet ratio, and/or the analysis parameter includes a combination parameter of a high fluorescence intensity cell percentage and a standard deviation of the red blood cell distribution width, especially a linear combination parameter, and/or the analysis parameter includes a large platelet ratio. Combination parameters of red blood cell ratio, average platelet volume and average red blood cell hemoglobin concentration, especially linear combination parameters; and/or the analysis parameters include combination parameters of small red blood cell ratio and platelet distribution width standard deviation, especially nonlinear combination parameters.
30. The method according to any one of claims 18 to 29, wherein the method further comprises:

    The blood routine parameters of the blood sample to be tested are displayed on the display device of the blood analyzer, especially blood routine parameters used to obtain analysis parameters related to the novel coronavirus infection.
31. A computer-readable storage medium applied to a blood analyzer, wherein a computer program is stored on the computer-readable storage medium, and is characterized in that the following steps are implemented when the computer program is executed by a processor:

    Obtain the blood routine parameters of the blood sample to be tested through the detection device of the blood analyzer;

    Obtain analysis parameters related to the novel coronavirus infection from the blood routine parameters of the blood sample to be tested;

    When the analysis parameters meet the preset conditions, it is instructed to display information related to the novel coronavirus infection on the display device of the blood analyzer.

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