WO2021177522A1 - Infectious respiratory virus-blocking composition and use thereof - Google Patents

Abstract

The present invention relates to a virus-blocking composition comprising sodium phosphate, and a virus-blocking mask filter comprising the composition.

Description

Composition for blocking infectious respiratory virus and use thereof

The present invention relates to a virus-blocking composition comprising sodium phosphate and a virus-blocking mask filter comprising the same.

Human infectious diseases include various diseases such as respiratory diseases, which are caused by bacteria, fungi, viruses, and the like. In particular, since most viruses have a size of 100 nm or less, it is not easy to completely filter them. Influenza caused by influenza virus infection occurs every year, and intermittent pandemics can kill millions of people. In 2009, a swine influenza outbreak occurred that killed tens of thousands of people worldwide.

Influenza viruses also infect animals, and birds and pigs act as intermediate host animals for influenza viruses. it is thought Inhalation of air containing the influenza virus can be a route of infection, especially for infected patients or persons working with animals. The air discharged from the breath, cough, or sputum of an infected patient contains countless viruses, and the virus is also emitted from animal feces. high.

A mask with an appropriate filter material is an effective means of preventing infection by airborne viruses. Recently, as a mask filter material, a non-woven material including an air filter that removes viruses or other microorganisms, a filter having a small diameter and using an electrostatic principle to block fine particles, etc. have been used. On the other hand, there has been a problem that the conventionally known virus blocking mask is insufficient in protecting or blocking infectious respiratory viruses having a diameter of 80-300 nm or less.

Accordingly, there is a need to develop a mask filter and mask material that can effectively block and defend even a small-sized infectious respiratory virus.

It is an object of the present invention to provide a composition for virus blocking comprising sodium phosphate.

Another object of the present invention is to provide a mask filter for virus blocking comprising the composition.

Another object of the present invention is to provide a method for manufacturing a mask filter for blocking viruses.

One aspect of the present invention relates to a composition for virus blocking comprising sodium phosphate.

In the present invention, “sodium phosphate” has a molecular formula of NaH ₂ PO ₄ , and is a chemical substance of colorless crystals and white powder. It is highly soluble in water, an aqueous solution is alkaline, and insoluble in alcohol. In general, sodium phosphate is one of the substances whose safety is ensured enough to be variously used in the food field, industrial field, other feed, medicine, toothpaste, and the like. In the food field, pH buffering agent, emulsification dispersant, food rancidity and fermentation inhibitor, noodle processing tissue improver, fish meat tenderizer elasticity enhancer, cheese emulsifier, canned food discoloration prevention, ion sequestrant, vermicelli noodles, pick-me-down immunity, nuts, tender meat, It is used for sugar pickling, grain processing, document processing, starch processing, sugar processing, quality improving agent (fish meat dough product, dairy product), volume increase of ice cream, food browning prevention and gloss. In the industrial field, it is used as a cleaning agent, water softener, metal surface treatment agent, synthetic rubber manufacturing, EPS expander, dye dispersion (penetrating agent), pesticide, reagent, printing, cleaning agent, detergent, water treatment agent, metal ion sequestrant, etc.

In the present invention, it was confirmed through specific experiments that sodium phosphate, which is already used for various purposes, has a significant effect in defense or blocking of an infectious respiratory virus having a diameter of 200 nm or less, more specifically, a size of 80 nm or less. Accordingly, the present invention intends to provide a virus-blocking composition containing sodium phosphate, which can easily obtain virus-blocking and protective effects by simply coating a virus-blocking article, such as a virus-blocking mask, with sodium phosphate.

Specifically, the composition may include sodium phosphate in a concentration of 0.5M or more and 10M or less. More specifically, it may include 1M or more and 10M or less, and more specifically, it may include 1M or more and 5M or less.

The virus may be an influenza virus.

In the present invention, “virus” refers to small particles that are smaller than bacteria that depend on the host and that are composed of a small number of proteins and nucleic acids necessary for survival and cannot be separated even by a bacterial filter. Viruses can be classified into plant viruses and animal viruses. Plant viruses include tobacco mosaic virus, cassava mosaic virus, potato Y virus, sugar beet yellow virus, cucumber mosaic virus, etc., and animal viruses include Japanese encephalitis virus, Dengue virus, yellow fever virus, polio virus, coxsackie virus, echo virus, hepatitis virus, rabies virus, influenza virus, adenovirus and the like.

In the present invention, "Byser blocking" refers to the action of inhibiting, preventing, or killing the virus, growth and/or activity of the virus. As a specific embodiment of the present invention, it may be to inhibit, prevent or kill the activity of a human respiratory infection virus, such as influenza.

In the present invention, “influenza” is RNA orthomyxoviruses and consists of three types A, B and C. Influenza viruses of types B and C are mainly human pathogens, whereas influenza A viruses infect a variety of birds and mammals in the wild, including humans, horses, marine mammals, pigs, ferrets and chickens. Influenza A viruses are subtyped based on allelic modifications in the antigenic regions of two genes encoding surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA), which are required for virus attachment and cell release. can be classified as Other major viral proteins include nucleoproteins, nucleocapsid structural proteins, membrane proteins (M1 and M2), polymerases (PA, PB and PB2) and non-structural proteins (NS1 and NS2). Currently, 16 subtypes of HA (H1-H16) and 9 NA (N1-N9) antigen variants are known in influenza A virus. Influenza virus subtypes can be further classified with reference to their phylogenetic groups. Phylogenetic analysis (Fouchier et al., 2005) discloses that the subdivision of HA is divided into two main groups: H1, H2, H5 and H9 subtypes of phylogenetic group 1 and H3, H4 and H7 of phylogenetic group 2.

Specifically, the virus may be an influenza virus of subtype H3N2 or H5N1.

More specifically, the H3N2 subtype influenza may be A/Hong Kong/68 (H3N2). The A/Hong Kong/68 (H3N2) is an influenza A virus isolated from Hong Kong and has serotypes of H3 and N2.

In addition, the H5N1 subtype influenza may be A/Viet Nam/1203/2004 (H5N1). The A/Viet Nam/1203/2004 (H5N1) is an influenza A virus that was isolated in Vietnam in 2004 as the 1203th virus and has H5 and N1 serotypes.

As a result of measuring the activity of the influenza virus attached to the filter coated with the composition containing the sodium phosphate in an embodiment of the present invention, it was confirmed that more than 99.9% of the influenza virus was inactivated (FIG. 3).

In one embodiment of the present invention, the amount of influenza virus that passed without being attached to the sodium phosphate-coated filter was small, and it was confirmed that a significant number of influenza viruses that passed were inactivated (FIG. 4).

The above results show that a significant level of influenza virus does not pass through a filter coated with sodium phosphate, and is mostly inactivated even if it passes.

In addition, in one embodiment of the present invention, as a result of treating the mouse with the virus that passed through the sodium phosphate-coated filter, a significantly lower amount of virus was detected in the lungs of the mouse, and it was confirmed that the survival rate of the mouse also increased (Fig. 5). ).

The above results show that the composition containing sodium phosphate of the present invention has an excellent effect on blocking viruses having a small size such as influenza virus.

Another aspect of the present invention relates to a virus-blocking mask filter comprising the virus-blocking composition.

In the present invention, "mask" refers to an object that covers the mouth and nose to prevent foreign substances such as germs or dust from penetrating into the body, and there may be a virus blocking mask for blocking and defending infectious viruses. The antiviral mask generally includes means to hold it in place over the user's face, a mask filter to block the virus, and through which the user's inhaled and/or exhaled air can pass through the filter material.

In the present invention, "mask filter" refers to the function of effectively protecting the user's respiratory system by effectively blocking the intrusion of various viruses through the respiratory tract.

Materials that can be used in the filter of the present invention can be used without limitation as long as it is a material for manufacturing a filter known in the art. fabrics such as cotton, cellulose, wool, polyolefin, polyester, polyamide, rayon, polyacrylonitrile, cellulose, acetate, polystyrene, polyvinyl, and any synthetic polymer that can be processed into fibers. and may include non-woven fabrics such as polypropylene, polyethylene, polyester, nylon, PET and PLA.

Specifically, the virus blocking composition may be coated on the surface of the mask filter.

The coating includes an act of coating a thin film on the surface of an object with a resin or the like, or applying a thin film by dropping a solution or a liquid substance and the like. Specifically, by dispensing sodium phosphate to the mask filter and drying it, it may be a mask filter in which sodium phosphate is thinly coated.

In the case of the mask filter coated with sodium phosphate in an embodiment of the present invention, it was confirmed that the size and number of pores in the filter were reduced, while the resistance of the mask filter was increased (FIG. ).

In one embodiment of the present invention, as a result of dispensing an aerosolized influenza virus on the mask filter after coating sodium phosphate, a significant number of viruses are attached to the filter and inactivated, and the virus that has passed through some filters is treated in mice In one result, it was confirmed that the activity of virus and the expression of inflammatory cytokines in the lungs of mice were significantly reduced compared to the filter not coated with sodium phosphate ( FIGS. 6 and 7 ).

In addition, in one embodiment of the present invention, as a result of treating the mouse with the virus that has passed through the sodium phosphate-coated mask filter, 100% survival rate appears in the mouse showing a high lethality rate even with a low concentration of virus treatment, and the change in body weight is almost It was confirmed that there is no (FIG. 8), which shows that the coating material using sodium phosphate of the present invention has an excellent virus blocking effect.

These results suggest that a mask filter coated with sodium phosphate can effectively block an infectious respiratory virus of a small size, and thus can be used as a mask filter for influenza virus blocking.

Another aspect of the present invention relates to a method for manufacturing a mask filter for virus blocking, comprising the step of coating sodium phosphate (sodium phosphate) on the mask filter.

The descriptions of 'sodium phosphate', 'mask filter', 'virus' and the like are the same as described above.

Specifically, the sodium phosphate may be coated on the mask filter at a concentration of 0.5 M or more and 10 M or less. More specifically, it may include 1M or more and 10M or less, and more specifically 1M or more and 5M or less.

In one embodiment of the present invention, sodium phosphate was sprayed on a filter having a small diameter used as a conventional mask filter, and a mask filter for blocking virus coated with sodium phosphate was manufactured.

Also, specifically, the virus may be influenza of H3N2 or H5N1 subtype. That is, the method may be a method of manufacturing a mask filter for blocking influenza of H3N2 or H5N1 subtype.

More specifically, the H3N2 subtype virus may be A/Hong Kong/68 (H3N2). The A/Hong Kong/68 (H3N2) is an influenza A virus isolated from Hong Kong and has serotypes of H3 and N2.

In addition, the H5N1 subtype influenza may be A/Viet Nam/1203/2004 (H5N1). The A/Viet Nam/1203/2004 (H5N1) is an influenza A virus that was isolated in Vietnam in 2004 as the 1203th virus and has H5 and N1 serotypes.

In an embodiment of the present invention, as a result of treating the aerosolized influenza virus after coating the mask filter with sodium phosphate, most viruses are attached to the filter and inactivated, and some viruses that have passed through the filter also have a concentration of sodium phosphate It was confirmed that the virus was either inactivated or non-lethal in a dependent manner ( FIGS. 3 to 8 ).

The antiviral composition comprising sodium phosphate of the present invention is effective in blocking and defending a small-sized infectious respiratory virus, and furthermore, it is harmless to the human body, so it can be used in various ways as a virus blocking application.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 shows an experimental design for confirming the virus blocking effect of a mask material coated with sodium phosphate.

2 shows the results of observing the mask filter coated with sodium phosphate under a microscope (A: control, B: 1M sodium chloride coating, C: 3M sodium chloride coating, D: 5M sodium chloride coating, E: mask filter pore size, F: pore counts of the mask filter, G: breathability of the mask filter).

3 shows the results of measuring the activity (Plaque assay) of the H3N2 virus attached to the sodium phosphate-coated filter (A: measurement of the activity of the virus attached to the filter, B: the result of the HA titer of the virus attached to the filter, C : Virus blocking rate of filter after spraying on filter with virus aerosolization).

4 shows the results of measuring the H3N2 virus activity that passed through the sodium phosphate-coated filter (A: measurement of the activity of the virus that passed the filter, B: After the virus that passed the filter was infected in the mouse, the lungs were collected to measure the amount of virus).

5 shows the results of measuring the inflammatory response and survival rate of mice infected with H3N2 virus that passed through the sodium phosphate-coated filter (A: measurement of inflammatory cytokines, B: survival rate of mice infected with H3N2 virus).

6 shows the results of measuring the activity of low concentration (2 mg/ml) H3N2 and H5N1 viruses attached to a sodium phosphate-coated filter (A: activity of H3N2 virus attached to filter, B: H3N2 virus passing through filter activity, C: H3N2 virus blocking rate by the filter, D: H5N1 virus activity attached to the filter, E: H5N1 virus activity through the filter, F: H5N1 virus blocking rate by the filter).

7 shows the results of measuring the activity of low-concentration H3N2 and H5N1 viruses passing through a sodium phosphate-coated filter (A: H3N2 virus, B: H5N1 virus).

8 shows the weight change and survival rate measurement results of mice infected with low-concentration H3N2 and H5N1 viruses passed through a sodium phosphate-coated filter (A: H3N2 mouse weight change rate, B: H3N2 mouse survival rate, C: H5N1 virus-infected mouse weight change rate) , D: survival of mice infected with H5N1 virus).

Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples are merely illustrative of the present invention, and the present invention is not limited by the following examples.

Example 1. Preparation of Sodium Phosphate Coated Mask Filter

1M, 3M, 5M sodium phosphate solutions (sodium phosphate and 1% Tween-20 in D.W) were prepared. After dispensing 2 ml of the sodium phosphate solution into a mask filter having a 100 mm dish size, it was incubated at 37° C. overnight. and dried overnight at 37°C.

In this way, the manufactured ring mask filter was observed with a scanning electron microscope, and the pore size and counts of the mask filter were measured.

As a result, it was confirmed that the pore size of the mask coated with sodium phosphate was smaller than that of the conventional commercially available control mask filter, and the number of pores of the mask filter decreased as the high salt was coated. On the other hand, the resistance of the mask filter was increased, and it was confirmed that it had a resistance of about KF94 commercially available in Korea (FIG. 2).

Experimental Example 1. Confirmation of virus blocking effect of sodium phosphate coated mask

1-1. H3N2 influenza virus blocking effect attached to the filter

After preparing 8 mg/ml of A/Hong Kong/68 (H3N2), 10 μl of A/Hong Kong/68 (H3N2) was dispensed into an aerosol machine to prepare an aerosolized virus. The aerosol was applied to a filter without salt coating (Bare), a filter coated with 1M sodium phosphate (1M SP), a filter coated with 3M sodium phosphate (3M SP), and a filter coated with 5M sodium phosphate (5M SP). The infected virus was sprayed. Thereafter, each virus adhering to the filter was collected.

In order to measure the amount of virus attached to the filter, MDCK cells were infected and a plaque assay was performed. Specifically, after culturing MDCK cells to about 150% in a 12-well plate, the virus suspension to be specifically quantified was diluted 10-fold from 10 ^{-1 to 10 6} and the diluted solution was inoculated by 0.25 mL per well. . After adsorbing the virus for about 30 minutes, overlay media was added. In this state, the plaques were counted after 2-3 days at 37° C. and 5% CO _{2 into a cell incubator.} At the same time as supplying nutrients, the overlay media made the medium in a gel state to prevent the virus propagated in the first infected cells from spreading through the medium and infecting the surrounding cells. To facilitate plaque counting, 300 μl/well of 0.25% glutarahldehyde was added to fix the cells, and after removing the overlay media, 400 μl of 1% cyristal violet was added per well. The number of plaques was counted, and the titer of the virus stock was expressed in plaque forming units per milliliter (PFU/mL), taking into account the inoculum and dilution factor. At this time, several dilution multiples were used to minimize errors in calculation. was used, and the titer was calculated by counting as a plate in which about 10 to 40 plaques were formed.

[Equation 1]

Plaque number x dilution factor x inoculum converted to mL = PFU/mL

As a result, it was confirmed that the activity of the virus attached to the filter coated with sodium phosphate was significantly reduced as the concentration of sodium phosphate increased, and 1M, 3M, and 5M sodium phosphate compared to the control (Bar) uncoated with sodium phosphate. It was confirmed that the viruses of this coated filter were inactivated by 99.997% (1M SP), 99.999% (3M SP), and 100% (5M SP), respectively (FIG. 3A).

The virus attached to the filter was recovered and reacted with 0.5% chicken red blood cells (cRBC) to observe a hemagglutination reaction. Specifically, 50 ul of a 2-fold diluted sample of virus stock was dispensed on a U-bottom-shaped 96-well microplate, 50 ul of red blood cell solution was added, and reacted at room temperature for about 1 hour. The erythrocytes in the wells where lysis did not occur were submerged and appeared in the form of distinct small dots, and the erythrocytes in the wells in which lysis had not occurred formed lattices and did not sink, showing a red color solution as a whole. The highest dilution factor at which dissolution occurred was determined as the HA titer. As a result, it was confirmed that the higher the concentration of sodium phosphate, the lower the hemagglutinin activity of the influenza virus (FIG. 3B).

In addition, the virus blocking rate of the filter was measured by measuring the amount of virus attached to the filter after aerosolizing the virus and spraying it on the filter. As a result, it was confirmed that the virus blocking rate increased as the filter was coated with high concentration of sodium phosphate (Fig. 3C). ).

1-2. H3N2 influenza virus blocking effect through filter

After preparing A/Hong Kong/68 (H3N2) 8 mg/ml, 10 μl was dispensed in an aerosol machine to prepare an aerosolized virus. The aerosol was applied to a filter without salt coating (Bare), a filter coated with 1M sodium phosphate (1M SP), a filter coated with 3M sodium phosphate (3M SP), and a filter coated with 5M sodium phosphate (5M SP). The infected virus was sprayed. Then, 300 μl of the virus passed through the filter was dissolved in 0.1M PBS, and virus activity was measured in all groups under the same conditions. For the measurement of the activity of the virus passing through the filter, refer to the method of Experimental Example 1-1.

As a result, it was confirmed that the activity of the virus passing through the filter coated with sodium phosphate was significantly reduced in a concentration-dependent manner of sodium phosphate (Fig. 4A).

In addition, the aerosolized virus in Experimental Example 1-1 and the virus that passed through a filter coated with 1M, 3M, and 5M sodium phosphate were collected, infected with 100 μl of each mouse, sacrificed on the 4th day of infection, and the lungs were collected. The amount of virus was observed.

As a result, the number of viruses passing through the filter coated with 1M, 3M, and 5M sodium phosphate was significantly lower than that of the control group (Bar). Specifically, it was confirmed that 12% (1M SP), 53.43% (3. SP), and 73% (5M SP) less viruses were detected compared to the control group (Bar), respectively (FIG. 4B).

Experimental Example 2. Confirmation of inflammatory response and survival rate of virus-infected mice

2-1. Confirmation of Inflammatory Response in H3N2 Virus Infected Mice

The virus that was aerosolized in Experimental Example 1 and the virus that passed through a filter coated with 1M, 3M, or 5M sodium phosphate were collected and infected with mice. On day 4 of infection, mice were sacrificed and spleens were collected to observe the inflammatory response.

96-well plates were coated with 400 ng/well of anti-mouse gamma interferon (IFN-γ capture antibody (BD Bioscience) in 100 μL of PBS overnight at 4° C. Assay diluent (BD bioscience, CA, USA) with 100 μL of 1 After blocking at 25°C for a period of time, the plate was washed 3 times with PBS 0.01% Tween 20. Then, 100 μL of a medium solution from which splenocytes were isolated was added per well (1:1, 1:5, 1:10 in assay diluent). ratio), and incubated for 2 hours at 25° C. After washing the plate 3 times with PBS 0.1 % Tween 20, 100 μL of detection antibody and SAv-HRP conjugated antibody were added per well and incubated at 25° C. for 1 hour After washing 5 times with PBS 0.05 % Tween 20, 100 μl of TMB substrate reagent (BD Biosciences, CA, USA) was added to the plate and reacted for 10 minutes. 50 μL of 2N sulfuric acid was added to the plate The reaction was stopped The plate was measured at 450 nm in an ELISA reader.

As a result, it was confirmed that the expression of IFN-γ in the splenocytes of mice infected with the virus passing through the filter coated with 3M and 5M sodium phosphate was significantly reduced compared to the control group (Bar) (FIG. 5A).

2-2. Confirmation of survival rate of H3N2 virus-infected mice

In addition to the mice from which the spleen was collected in Experimental Example 2-1, survival rates were observed in the remaining mice.

As a result, mice treated with the virus of the control group (Bar) that passed through the filter uncoated with salt all died within 6 days, whereas in mice treated with the virus that passed through the filter coated with 3M and 5M sodium phosphate, It was confirmed that the survival rates of 20% and 80% or more, respectively, were significantly increased (FIG. 5B).

Experimental Example 3. Blocking effect of low-concentration virus attached to sodium phosphate-coated filter

3-1. Measurement of the activity of H3N2 influenza virus attached to and passed through the filter

The aerosolized H3N2 virus and the H3N2 virus that adhered to and passed through the 5M sodium phosphate-coated filter were collected in the same manner as in Experimental Example 1, respectively, and the virus activity was evaluated. However, a low concentration of 2 mg/ml of virus was used.

As a result, the H3N2 virus attached to the filter coated with sodium phosphate was significantly inactivated compared to the control (Bare) that was not coated with sodium phosphate. (Figs. 6A and 6B).

In addition, the virus was aerosolized and sprayed on the filter, and then the amount of virus attached to the filter was measured, confirming that the filter had an excellent virus blocking rate (FIG. 6C).

3-2. Measurement of the activity of H5N1 influenza virus attached to and passed through the filter

The aerosolized H5N1 virus and the H5N1 virus that adhered to and passed through the filter coated with 5M sodium phosphate were collected in the same manner as in Experimental Example 1, respectively, and the virus activity was evaluated. However, a low concentration of 2 mg/ml of virus was used.

As a result, it was confirmed that the H5N1 virus attached to the sodium phosphate-coated filter was significantly inactivated compared to the control (Bar), and 99% or more of the virus was inactivated even in the virus that passed without being attached to the filter ( FIGS. 6D and 6E ). In addition, it was confirmed that the virus blocking rate was excellent through the amount of virus attached to the filter (FIG. 6F).

3-3. H3N2 and H5N1 influenza virus blocking effect through filter

The aerosolized virus in Experimental Examples 3-1 and 3-2 and the H3N2 virus and H5N1 virus that passed through a filter coated with 5M sodium phosphate were collected, respectively, and 100 μl of each of the mice was infected. On day 4 of infection, mice were sacrificed and lungs were collected to observe the amount of virus.

As a result, the number of viruses passing through the filter coated with 5M sodium phosphate was significantly lower than that of the control group (Bar). Specifically, it was confirmed that 92% and 96% less H3N2 virus and H5N1 virus were detected, respectively, compared to the control group (Bar) (FIG. 7).

Experimental Example 4. Confirmation of survival rate of mice infected with low-concentration H3N2 and H5N1 influenza virus

In addition to the mice sacrificed to collect lungs in Experimental Example 3-3, body weight change and survival rates were observed in the remaining mice.

As a result, the mice treated with the low-concentration H3N2 virus of the control group (Bar) passed through the filter uncoated with salt showed 100% lethality, but mice treated with the low-concentration H3N2 virus that passed through the filter coated with 5M sodium phosphate showed 100% survival rate ( FIGS. 8A and 8B ).

In addition, in mice treated with a low-concentration H5N1 virus of the control group (Bar) that passed through a filter uncoated with salt, weight loss due to virus infection appeared, while H5N1 virus that passed through a filter coated with 5M sodium phosphate was treated. There was almost no weight loss in mice, and it was confirmed that 100% survival rate was shown ( FIGS. 8C and 8D ).

The above results show that the virus blocking effect on the filter coated with sodium phosphate is excellent, so the composition containing the sodium phosphate can be used as an infectious respiratory virus blocking and protective purpose, and it is possible to block viruses as well as mask filters. It can be used by coating various materials, articles, etc.

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

Claims (10)

1. A composition for virus blocking comprising sodium phosphate.
2. According to claim 1,

   The composition is sodium phosphate 0.5M or more and 10M or less of the concentration of the composition for virus blocking.
3. According to claim 1,

   Wherein the virus is influenza of H3N2 or H5N1 subtype, virus blocking composition.
4. 4. The method of claim 3,

   Influenza of the H3N2 subtype is A/Hong Kong/68 (H3N2), a composition for virus blocking.
5. 4. The method of claim 3,

   Influenza of the H5N1 subtype is A/Viet Nam/1203/2004 (H5N1), a composition for virus blocking.
6. A mask filter for virus blocking comprising the composition for blocking any one of claims 1 to 5.
7. 7. The method of claim 6,

   The virus-blocking composition is coated on the mask filter surface, the virus-blocking mask filter.
8. A method of manufacturing a mask filter for virus blocking, including; coating the mask filter with sodium phosphate.
9. 9. The method of claim 8,

   The method of manufacturing a mask filter for virus blocking, wherein the sodium phosphate is coated on the mask filter at a concentration of 0.5M or more and 10M or less.
10. 9. The method of claim 8,

    The virus is H3N2 or H5N1 subtype influenza, the method for manufacturing a mask filter for virus blocking.

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