WO2021164529A1

WO2021164529A1 - Detection chip, operation method therefor and detection system

Info

Publication number: WO2021164529A1
Application number: PCT/CN2021/074625
Authority: WO
Inventors: 胡涛; 崔皓辰; 袁春根; 李婧; 申晓贺; 胡立教; 甘伟琼
Original assignee: 京东方科技集团股份有限公司; 北京京东方健康科技有限公司
Priority date: 2020-02-20
Filing date: 2021-02-01
Publication date: 2021-08-26
Also published as: CN113275045A

Abstract

A detection chip (301), an operation method (800) therefor and a detection system (300); the detection chip (301) comprises: a liquid storage layer (101), a first pierceable sealing layer (102), a second pierceable sealing layer (103), a first sealing cover (104), a flow channel layer (105), and a second sealing cover (106); the liquid storage layer (101) is provided with an opposite first surface (1011) and second surface (1012), and comprises a liquid storage chamber (1013), the liquid storage chamber (1013) having a first opening (1014) at a first surface (1011) and a second opening (1015) at a second surface (1012); the first pierceable sealing layer (102) seals the first opening (1014) of the liquid storage chamber (1013); the second pierceable sealing layer (103) seals the second opening (1015) of the liquid storage chamber (1013); the first sealing cover (104) is located at a side of the first pierceable sealing layer (102) that is far from the liquid storage layer (101); the flow channel layer (105) is located at a side of the second pierceable sealing layer (103) that is far from the liquid storage layer (1013), and comprises a through-hole (1051) and a liquid flow channel (1052) which communicates with the through-hole (1051); and the second sealing cover (106) seals the opening of the through-hole (1051) on a surface of the flow channel layer (105) far from the liquid storage layer (101). The detection chip (301) can conveniently dilute or mix a liquid pre-stored within the liquid storage chamber (1013).

Description

Detection chip and its operation method and detection system

This application claims the priority of the Chinese patent application No. 202010104017.7 filed on February 20, 2020. For all purposes, the content of the above-mentioned Chinese patent application is quoted here in full as a part of this application.

Technical field

The embodiments of the present disclosure relate to a detection chip, an operation method thereof, and a detection system.

Background technique

Microfluidics is a technology that precisely controls and manipulates micro-scale fluids. It can integrate basic operation units such as sample, reaction, separation, and detection in the inspection and analysis process into a micro-nano-scale chip, and complete it automatically Analyze the whole process. Microfluidic technology has the advantages of low sample consumption, fast detection speed, simple operation, multi-functional integration, small size and easy portability, and has great potential for applications in biology, chemistry, medicine and other fields.

Summary of the invention

At least one embodiment of the present disclosure provides a detection chip, which includes:

The liquid storage layer has opposite first and second surfaces, and includes a liquid storage chamber, wherein the liquid storage chamber has a first opening on the first surface and a second opening on the second surface ；

A first pierceable sealing layer to seal the first opening of the liquid storage chamber;

A second pierceable sealing layer to seal the second opening of the liquid storage chamber;

The first sealing cover is located on the side of the first pierceable sealing layer away from the liquid storage layer, and is movably arranged to expose or seal at least the first pierceable sealing layer and the first The overlapping part of the opening;

The flow channel layer is located on the side of the second pierceable sealing layer away from the liquid storage layer, and includes a through hole and a liquid flow channel communicating with the through hole, wherein the through hole exposes at least the The part where the second pierceable sealing layer overlaps with the second opening; and

The second sealing cover is located on the side of the flow channel layer away from the liquid storage layer, and seals the opening of the through hole on the surface of the flow channel layer away from the liquid storage layer.

For example, in the detection chip according to at least one embodiment of the present disclosure, the first sealing cover can be elastically deformed.

For example, in the detection chip according to at least one embodiment of the present disclosure, the second sealing cover can be elastically deformed, and within the elastic deformation range of the second sealing cover, it is allowed to be perpendicular to the second sealing cover. When an external force acts on the second sealing cover, it can further act on the second pierceable sealing layer to destroy the second pierceable sealing layer.

For example, in the detection chip according to at least one embodiment of the present disclosure, in a case where the first sealing cover seals at least a portion where the first pierceable sealing layer overlaps the first opening, the first The orthographic projection of the sealing cover on the liquid storage layer at least partially overlaps the first opening.

For example, in the detection chip according to at least one embodiment of the present disclosure, the liquid flow channel of the flow channel layer at least partially includes a hollow area in the flow channel layer.

For example, the detection chip according to at least one embodiment of the present disclosure further includes an adhesive layer,

Wherein, the adhesive layer is between the second sealing cover and the flow channel layer to connect the second sealing cover and the flow channel layer.

For example, in the detection chip according to at least one embodiment of the present disclosure, the adhesive layer includes a hollow area, and

The opening of the through hole on the surface of the flow channel layer away from the liquid storage layer is within the shape of the orthographic projection of the hollow area on the flow channel layer.

For example, in the detection chip according to at least one embodiment of the present disclosure, the liquid storage chamber includes a through hole in the liquid storage layer.

At least one embodiment of the present disclosure further provides a detection system, including a tester and the test chip according to any embodiment of the present disclosure, wherein the tester includes a chip mounting structure, and the chip mounting structure is used for mounting The detection chip.

For example, in the detection system according to at least one embodiment of the present disclosure, the detection instrument further includes a liquid injection device,

Wherein, the liquid injection device includes a movable end and a liquid injection structure,

The movable end portion is configured to pierce the first pierceable sealing layer when the detection chip is mounted on the chip mounting structure, and

The liquid injection structure is configured to inject a target liquid into the liquid storage chamber.

For example, in the detection system according to at least one embodiment of the present disclosure, the detection instrument further includes a first action device,

Wherein, the first acting device includes a movable first end,

The first end portion is configured to apply a force to the first sealing cover of the detection chip mounted on the chip mounting structure.

For example, in the detection system according to at least one embodiment of the present disclosure, the detection instrument further includes a second action device,

Wherein, the second acting device includes a movable second end,

The second end portion is configured to apply a force to the second sealing cover of the detection chip mounted on the chip mounting structure.

For example, in the detection system according to at least one embodiment of the present disclosure, the detector further includes a gas operating device,

Wherein, the gas operating device includes a gas channel, and is configured to blow gas or inhale gas through the gas channel.

At least one embodiment of the present disclosure also provides a method for operating the detection chip according to any embodiment of the present disclosure, which includes:

Move the first sealing cover to pierce the first pierceable sealing layer to inject the target liquid;

After injecting the target liquid, moving the first sealing cover to seal at least the overlapping portion of the first pierceable sealing layer and the first opening;

Applying a force on the second sealing cover to pierce the second pierceable sealing layer, so that the liquid flow channel is in liquid communication with the liquid storage chamber; and

The liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer is driven so that the liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer Flow into the liquid flow channel or cause the liquid in the liquid flow channel to flow back into at least one of the liquid storage chamber and the through hole of the flow channel layer.

For example, in the method according to at least one embodiment of the present disclosure, the liquid in at least one of the liquid storage chamber and the through hole of the flow channel layer is driven so that the liquid storage chamber and the flow The liquid in at least one of the through holes of the channel layer flows into the liquid channel or causes the liquid in the liquid channel to flow back into at least one of the liquid storage chamber and the through holes of the channel layer One, including:

Press at least one of the first sealing cover and the second sealing cover so that the liquid in at least one of the liquid storage chamber and the through hole of the flow channel layer flows into the liquid flow channel ;as well as

At least one of the first sealing cover and the second sealing cover is released, so that the liquid in the liquid flow channel flows back into the liquid storage chamber and the through hole of the flow channel layer at least Within one.

Extracting the gas in the liquid flow channel so that the liquid in at least one of the liquid storage chamber and the through hole of the flow channel layer flows into the liquid flow channel; and

Gas is injected into the liquid flow channel, so that the liquid in the liquid flow channel flows back into at least one of the liquid storage chamber and the through hole of the flow channel layer.

For example, the method according to at least one embodiment of the present disclosure further includes:

Press at least one of the first sealing cover and the second sealing cover so that the liquid in at least one of the liquid storage chamber and the through hole of the flow channel layer flows into the liquid flow channel .

Description of the drawings

In order to explain the technical solutions of the embodiments of the present disclosure more clearly, the following will briefly introduce the drawings of the embodiments. Obviously, the drawings in the following description only refer to some embodiments of the present disclosure, rather than limiting the present disclosure. .

Fig. 1 is a schematic structural diagram of a detection chip according to at least one embodiment of the present disclosure;

FIG. 2 is an exploded schematic diagram of the detection chip shown in FIG. 1;

FIG. 3 is another exploded schematic diagram of the detection chip shown in FIG. 1;

Fig. 4 is a schematic block diagram of a detection system according to at least one embodiment of the present disclosure;

5 is a schematic diagram of a liquid injection operation performed by the liquid injection device of the detection system according to at least one embodiment of the present disclosure;

FIG. 6 is a schematic diagram of force application operations performed by the first acting device and the second acting device of the detection system according to at least one embodiment of the present disclosure;

FIG. 7 is a schematic diagram of performing gas action operation by the gas operation device of the detection system according to at least one embodiment of the present disclosure;

FIG. 8 is a flowchart of a method of operating a detection chip according to at least one embodiment of the present disclosure.

Detailed ways

In order to make the objectives, technical solutions, and advantages of the embodiments of the present disclosure clearer, the technical solutions of the embodiments of the present disclosure will be described clearly and completely in conjunction with the accompanying drawings of the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, rather than all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by a person of ordinary skill in the art without creative labor are within the protection scope of the present disclosure.

Unless otherwise defined, the technical terms or scientific terms used in the present disclosure shall have the usual meanings understood by those with ordinary skills in the field to which this disclosure belongs. The "first", "second" and similar words used in the present disclosure do not indicate any order, quantity, or importance, but are only used to distinguish different components. "Include" or "include" and other similar words mean that the element or item appearing before the word covers the elements or items listed after the word and their equivalents, but does not exclude other elements or items. Similar words such as "connected" or "connected" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "Down", "Left", "Right", etc. are only used to indicate the relative position relationship. When the absolute position of the described object changes, the relative position relationship may also change accordingly.

In order to keep the following description of the embodiments of the present disclosure clear and concise, the present disclosure omits detailed descriptions of known functions and known components.

At present, none of the detection chips on the market, such as microfluidic chips, are equipped with a sample mixing structure, and it is impossible to mix or dilute the test sample or reagent with the pre-stored liquid. In addition, the current sample mixing or dilution needs to be performed manually, which is not conducive to the popularization of diagnosis and the realization of rapid automated diagnosis in POCT (point-of-care testing).

At least one embodiment of the present disclosure provides a detection chip that can conveniently dilute or mix samples or reagents with the liquid pre-stored in the liquid storage chamber of the detection chip, thereby facilitating the development of biochemical, immune, and molecular reagent reactions. conduct. In addition, the detection chip processing technology according to the embodiments of the present disclosure is simple, low in cost, and convenient for mass production and application.

In addition, at least one embodiment of the present disclosure also provides a detection system including a detection chip and a method of operating the detection chip.

FIG. 1 is a schematic structural diagram of a detection chip according to at least one embodiment of the present disclosure, and FIG. 2 is an exploded schematic diagram of the detection chip shown in FIG. 1. As shown in Figures 1 and 2, the detection chip according to at least one embodiment of the present disclosure includes a liquid storage layer 101, a first pierceable sealing layer 102, a second pierceable sealing layer 103, a first sealing cover 104, and a fluid The road layer 105 and the second sealing cover 106.

The liquid storage layer 101 has a first surface 1011 and a second surface 1012 opposite to each other, and includes a liquid storage chamber 1013. The liquid storage chamber 1013 has a first opening 1014 on the first surface 1011 and a second opening 1015 on the second surface 1012. In FIGS. 1 and 2, the liquid storage layer 101 is exemplarily shown in a columnar shape, but it should be understood that the embodiments of the present disclosure are not limited thereto. For example, in other embodiments, according to actual requirements, the liquid storage layer 101 may have any suitable shape, such as a rectangular parallelepiped shape, which is not limited in the embodiments of the present disclosure.

The liquid storage chamber 1013 may include a through hole located in the liquid storage layer 101. For example, the through hole may penetrate the liquid storage layer 101 in a direction perpendicular to the first surface 1011 and/or the second surface 1012. For example, in FIGS. 1 and 2, the liquid storage chamber 1013 is exemplarily shown as a cylindrical shape with a circular cross section in a direction parallel to the first surface and/or the second surface 1012. However, it should be understood that the embodiments of the present disclosure are not limited thereto. In other embodiments, according to actual requirements, the liquid storage chamber 1013 may have any suitable shape, for example, a polygonal cross-section in a direction parallel to the first surface and/or the second surface 1012, etc. The embodiments of the present disclosure are suitable for This is not limited.

The shape of the first opening 1014 may be the same as or different from the shape of the second opening 1015, which is not limited in the embodiment of the present disclosure. In addition, the orthographic projection of the first opening 1014 on the second surface 1012 may at least partially overlap or not overlap with the second opening 1015, which is not limited in the embodiment of the present disclosure.

The first pierceable sealing layer 102 is disposed on the first surface 1011 of the liquid storage layer 101 and seals the first opening 1014 of the liquid storage chamber 101. The orthographic projection of the first pierceable sealing layer 102 on the first surface 1011 may cover the first opening 1014. The first pierceable sealing layer 102 can be integrally formed with the liquid storage layer 101, or the first pierceable sealing layer 102 can be bonded to the liquid storage layer 101 by hot pressing, ultrasonic welding, photosensitive adhesive bonding, chemical solvent bonding, or For connection by means of laser welding, etc., the embodiments of the present disclosure do not limit this.

The second pierceable sealing layer 103 is disposed on the second surface 1012 of the liquid storage layer 101 and seals the second opening 1015 of the liquid storage chamber 101. The orthographic projection of the second pierceable sealing layer 103 on the second surface 1012 may cover the second opening 1015. The second pierceable sealing layer 103 can be integrally formed with the liquid storage layer 101, or the second pierceable sealing layer 103 can be bonded to the liquid storage layer 101 by hot pressing, ultrasonic welding, photosensitive adhesive bonding, chemical solvent bonding, or For connection by means of laser welding, etc., the embodiments of the present disclosure do not limit this.

The first sealing cover 104 is on the side of the first pierceable sealing layer 102 away from the liquid storage layer 101, and is movably disposed to expose or seal at least the overlapping portion of the first pierceable sealing layer 102 and the first opening 1014 Therefore, the liquid storage chamber 1013 can be operated through the first opening 1014. For example, when a target liquid (such as a sample, solvent, diluent, etc.) is injected into the liquid storage chamber 1013, the first sealing cover 104 can be moved to expose at least the first pierceable sealing layer 102 and the first opening 1014 The overlapping part, and when mixing is performed after injecting the target liquid (such as the sample to be tested, solvent, diluent, etc.) into the liquid storage chamber 1013, the first sealing cover 104 can be moved to seal at least the first pierceable seal The portion where the layer 102 overlaps the first opening 1014. It should be understood that the shape of the first sealing cover 104 in FIG. 1 and FIG. 2 is only exemplary, and the embodiment of the present disclosure is not limited thereto.

In an exemplary embodiment, the liquid storage layer 101 may be cylindrical, the first sealing cover 104 may have a top wall and an annular skirt extending downward from the top wall and surrounding the top wall, and the inside of the skirt may have threads. The outside of the side wall of the liquid storage layer 101 may have threads that can be matched with the threads inside the skirt of the first sealing cover 104, so that the first sealing cover 104 can form a separable threaded connection with the liquid storage layer 101 to be able to be Move, thereby exposing or sealing at least the overlapping portion of the first pierceable sealing layer 102 and the first opening 1014.

In another exemplary embodiment, the first sealing cover 104 may be hermetically connected to at least one of the liquid storage layer 101 and the first pierceable sealing layer 102 by an adhesive, wherein the adhesive is reusable . By peeling off the first sealing cover 104 from at least one of the liquid storage layer 101 and the first pierceable sealing layer 102, at least the overlapping portion of the first pierceable sealing layer 102 and the first opening 1014 can be exposed. After that, the first sealing cover 104 is again bonded to at least one of the liquid storage layer 101 and the first pierceable sealing layer 102 through the reusable adhesive, and can seal at least the first pierceable sealing layer 102 and The overlapped portion of the first opening 1014.

In another exemplary embodiment, the first sealing cover 104 may be fixed on the first surface 1011 of the liquid storage layer 101 by, for example, a pin on one side, and then the dowel may be wound on the first surface 1011 of the liquid storage layer 101. It rotates and is thus dialed to expose or seal at least the overlapping portion of the first pierceable sealing layer 102 and the first opening 1014.

The flow channel layer 105 is on the side of the second pierceable sealing layer 103 away from the liquid storage layer 101 and includes a through hole 1051 and a liquid flow channel 1052 communicating with the through hole 1051. The through hole 1051 exposes at least the overlapping portion of the second pierceable sealing layer 103 and the second opening 1015, thereby allowing the through hole 1051 to communicate with the second opening 1015 after the second pierceable sealing layer 103 is pierced. For example, the orthographic projection of the opening of the through hole 1051 on the second surface 1012 of the surface of the flow channel layer 105 close to the liquid storage layer 101 at least partially overlaps the second opening 1015. Although the liquid flow channel 1052 is shown in dotted lines in FIGS. 1 and 2, it should be understood that the liquid flow channel 1052 is continuous. The shape of the liquid flow channel 1052 shown in FIG. 1 and FIG. 2 is only exemplary, and the embodiment of the present disclosure does not limit this. For example, the liquid flow channel 1052 is used to transport liquid, for example, the liquid from the liquid storage chamber 1013 is transported to a desired part of the flow channel layer 105, such as a reaction zone, a detection zone, a waste liquid collection zone, an outlet, etc., the implementation of the present disclosure The example does not restrict this.

The flow channel layer 105 and the liquid storage layer 101 may form a liquid-tight connection, for example. For example, the flow channel layer 105 and the liquid storage layer 101 may be connected to each other in a liquid-tight manner by hot pressing, ultrasonic welding, photosensitive adhesive bonding, chemical solvent bonding, or laser welding, which is not limited in the embodiments of the present disclosure.

The second sealing cover 106 is on the side of the flow channel layer 105 away from the liquid storage layer 101 and seals the opening of the through hole 1051 on the surface of the flow channel layer 105 away from the liquid storage layer 101. For example, the second sealing cover 106 may only cover the opening of the through hole 1051 on the surface of the flow channel layer 105 away from the liquid storage layer 101, or the second sealing cover 106 may cover the surface of the flow channel layer 105 away from the liquid storage layer 101. The disclosed embodiment does not limit this. For example, the second sealing cover 106 can be connected to the flow channel layer 105 by hot pressing, ultrasonic welding, photosensitive adhesive bonding, chemical solvent bonding, or laser welding to seal the through hole 1051 in the flow channel layer 105 away from the liquid storage layer. The opening on the surface of 101 is not limited by the embodiment of the present disclosure.

In some embodiments, the first sealing cover 104 can be elastically deformed. For example, in the case where the second pierceable sealing layer 103 is pierced so that the liquid storage chamber 1013 communicates with the through hole 1051 in the flow channel layer 105 and the liquid flow channel 1052, the first sealing cover 104 may face The liquid storage chamber 1013 applies force to press the first sealing cover 104, thereby increasing the air pressure in the liquid storage chamber 1013, thereby driving the liquid in the liquid storage chamber 1013 to flow into the through hole 1051, and then into the liquid flow channel 1052, and then After the force is removed to release the first sealing cover 104, the first sealing cover 104 can be restored to its original shape without being damaged, so that the liquid in the liquid flow channel 1052 flows back to at least one of the liquid storage chamber 1013 and the through hole 1051. Within one. For example, the pressing and releasing operations can be repeated by applying a force on the first sealing cover 104 toward the liquid storage chamber 1013, so that the liquid can be mixed more uniformly.

In some embodiments, the second sealing cover 106 can be elastically deformed, and within the elastic deformation range of the second sealing cover 106, it is allowed to further be able to further when an external force perpendicular to the second sealing cover 106 acts on the second sealing cover 106. Acting on the second pierceable sealing layer 103 to destroy the second pierceable sealing layer 103, thereby making the through hole 1051 communicate with the second opening 1015.

The first sealing cover 104 and the second sealing cover 106 may be formed of the same or different materials, which is not limited in the embodiment of the present disclosure. For example, the material of the first sealing cover 104 and the second sealing cover 106 may include at least one of the materials listed below to have better elasticity and strength, so as to be able to return to the original state after elastic deformation: Polyethylene Terephthalate (PET), Polystyrene (PS), Poly(methyl methacrylate) (PMMA), Polypropylene (PP), Polycarbonate (Polycarbonate) , PC) or a combination of the foregoing materials.

In some embodiments, when the first sealing cover 104 seals at least the overlapping portion of the first pierceable sealing layer 102 and the first opening 1014, the orthographic projection of the first sealing cover 104 on the liquid storage layer 101 and the first An opening 1014 overlaps at least partially. As shown in FIG. 1, in the case where the first sealing cover 104 seals at least the overlapping portion of the first pierceable sealing layer 102 and the first opening 1014, the orthographic projection of the first sealing cover 104 on the liquid storage layer 101 may be the same as The first opening 1014 completely overlaps. However, it should be understood that in the case where the first sealing cover 104 seals at least the overlapping portion of the first pierceable sealing layer 102 and the first opening 1014, the orthographic projection of the first sealing cover 104 on the liquid storage layer 101 may also be the same as A part of the first opening 1014 overlaps, which is not limited in the embodiment of the present disclosure.

In some embodiments, the liquid flow channel 1052 of the flow channel layer 105 at least partially includes a hollow area in the flow channel layer 105. For example, the above-mentioned hollow area may be formed in at least one of the following positions: on the surface of the flow channel layer 105 away from the liquid storage layer 101 or inside the flow channel layer 105. In some embodiments, the above-mentioned hollow area is formed on the surface of the flow channel layer 105 away from the liquid storage layer 101, and the second sealing cover 106 in addition to sealing the openings of the through holes 1051 on the surface of the flow channel layer 105 away from the liquid storage layer 101 In addition, these hollow areas can also be sealed to form a liquid flow channel 1052. It should be understood that the liquid flow channel 1052 shown in FIG. 1 is only exemplary. In other embodiments, the liquid flow channel 1052 may have other shapes, numbers, etc., which are not limited in the embodiments of the present disclosure.

Fig. 3 is another exploded schematic diagram of the detection chip shown in Fig. 1. The structure of the detection chip shown in FIG. 3 is basically the same as the structure of the detection chip shown in FIG. 2, except that the detection chip of FIG. 3 also includes an adhesive layer 107. The adhesive layer 107 is between the second sealing cover 106 and the flow channel layer 105 to connect the second sealing cover 106 and the flow channel layer 105.

For example, the adhesive layer 107 may include an adhesive material such as an acrylic adhesive. For example, it may be implemented as an adhesive coating or as a double-sided tape. For example, the adhesive layer 107 and the second sealing cover 106 have substantially the same outline, so the adhesive layer 107 can enable the second sealing cover 106 and the flow channel layer 105 to achieve a firm combination.

In some embodiments, the second sealing cover 106 seals the opening of the through hole 1051 on the surface of the flow channel layer 105 away from the liquid storage layer 101 and the liquid channel 1052 on the surface of the flow channel layer 105 away from the liquid storage layer 101. In the case of a hollow structure, the adhesive layer 107 can expose the opening of the through hole 1051 on the surface of the flow channel layer 105 away from the liquid storage layer 101 and the hollow of the liquid channel 1052 on the surface of the flow channel layer 105 away from the liquid storage layer 101 structure. That is, the adhesive layer 107 may include a hollow area 1071, the shape of the hollow area 1071 and the opening of the through hole 1051 on the surface of the flow channel layer 105 away from the liquid storage layer 101 and the liquid channel 1052 in the flow channel layer 105 away from the liquid storage layer. The shape of the hollow structure on the surface of the layer 101 is the same or substantially the same, thereby facilitating the formation of the hollow structure of the second sealing cover 106 and the liquid flow channel 1052 on the surface of the flow channel layer 105 away from the liquid storage layer 101 for, for example, liquid flow and / Or space for reagent reaction. In other embodiments, when the second sealing cover 106 only covers the opening of the through hole 1051 on the surface of the flow channel layer 105 away from the liquid storage layer 101, the hollow area 1071 of the adhesive layer 107 may only expose the through hole. 1051 is an opening on the surface of the flow channel layer 105 away from the liquid storage layer 101.

With the detection chip according to each embodiment of the present disclosure, the sample or reagent can be easily diluted or mixed with the liquid pre-stored in the liquid storage chamber, which is beneficial to the progress of biochemical, immune, and molecular reagent reactions. In addition, the detection chip processing technology according to the embodiments of the present disclosure is simple, low in cost, and convenient for mass production and application.

Fig. 4 is a schematic block diagram of a detection system according to at least one embodiment of the present disclosure. FIG. 5 is a schematic diagram of a liquid injection operation performed by the liquid injection device of the detection system according to at least one embodiment of the present disclosure. Fig. 6 is a schematic diagram of force application operations performed by the first acting device and the second acting device of the detection system according to at least one embodiment of the present disclosure. FIG. 7 is a schematic diagram of gas action operation performed by the gas operation device of the detection system according to at least one embodiment of the present disclosure.

As shown in FIG. 4, a detection system 300 according to at least one embodiment of the present disclosure includes at least one detection chip 301 and a detection device (or detection device) 310. For example, the detection device 310 includes a base and a chip disposed on the base. The installation structure 302 may further include a detection component provided on the base. For example, the detection component includes a signal transmitter and a signal receiver, and the two are spaced apart to allow the detection chip 301 to be located between the two. In the transmission path. For example, the detection component is, for example, a photodetection component, including a light emitting element and a photo sensor, the light emitting element is, for example, a light emitting diode, and the photo sensor is, for example, a photodiode, such as a silicon photodiode. In addition, in order to achieve detection requirements, the detection assembly may also include, for example, optical path control components, such as lenses, mirrors, and the like. In addition, as required, the detector 310 may further include a controller (such as a central processing unit, a programmable logic controller, etc.), a power supply, a signal transceiver, a modem, etc., so that the detector can also interact with other terminals (such as mobile phones). , Tablet computers, etc.) to communicate, so that the detection can be controlled through these terminals, and the detection results can be displayed. The chip mounting structure 302 is used for mounting the detection chip 301. The detection chip 301 can be a detection chip according to any embodiment of the present disclosure. For example, the detection chip 301 can be provided in combination with the detector 310. This combination does not require that the detection chip must be installed in the chip mounting structure 302 of the detector 310; the combination A plurality of detection chips may be included in the detection chip, and these detection chips may have the same specifications (for example, size, included liquid, etc.), or may have different specifications, which are not limited in the embodiments of the present disclosure.

In some embodiments, the chip mounting structure 302 is movable on the base, so that the detection chip 301 mounted on the chip mounting structure 302 is moved. For example, the chip mounting structure 302 can be moved relative to the detection component in order to perform a detection operation on the liquid in the detection chip 301. The chip mounting structure 302 may be in various forms, for example, may include a frame to receive the detection chip 301, and may at least partially fix the detection chip 301 through a limiting structure or a clamping structure.

As shown in FIG. 4, the detector 310 of the detection system 300 according to at least one embodiment of the present disclosure may further include a liquid injection device 303. FIG. 5 is a schematic diagram of the liquid injection operation performed by the liquid injection device 303. As shown in FIG. 5, the liquid injection device 303 may include a movable end portion 3031 and a liquid injection structure 3032. For example, the liquid injection device 303 can be installed at the end of the screw, and the screw can be driven by a screw pair. For example, the liquid injection device 303 can be installed on a rod driven by a pneumatic or a cam, so that the liquid injection device 303 can be Move in the vertical direction in the figure.

The movable end portion 3031 is configured to pierce the first pierceable sealing layer 102 when the detection chip 301 is mounted on the chip mounting structure 302. It should be understood that when the first pierceable sealing layer 102 is pierced through the movable end portion 3031, the first sealing cover 104 has been moved so that at least the first pierceable sealing layer 102 overlaps the first opening 1014. Part of it is exposed. In FIG. 5, for clarity of illustration, the first sealing cover 104 that has been moved is not shown. However, it should be understood that in some embodiments, when the first sealing cover 104 is moved to expose the overlapped portion of the first pierceable sealing layer 102 and the first opening 1014, it can still be connected to other parts of the detection chip 301 (for example, storage). The liquid layer 101) is kept connected, so as to avoid the loss or contamination of the first sealing cover 104, and after injecting the target liquid (such as the sample to be tested, solvent, diluent, etc.) into the liquid storage chamber of the liquid storage layer 101, it can be conveniently The first sealing cover 104 is moved to seal the overlapping portion of the first pierceable sealing layer 102 and the first opening 1014.

The movable active end 3031 includes but is not limited to a tip, as long as it can pierce the first pierceable sealing layer 102.

The liquid injection structure 3032 is configured to inject a target liquid (such as a sample to be tested, a solvent, a diluent, etc.) into the liquid storage chamber 1013. The liquid injection structure 3032 can store the target liquid therein, and the liquid injection structure 3032 can be in fluid communication with the movable acting end 3031, so that the first pierceable sealing layer 102 is pierced at the movable acting end 3031 , The target liquid (such as the sample to be tested, solvent, diluent, etc.) stored in the liquid injection structure 3032 can enter the liquid storage chamber 1031 through the movable end portion 3031.

As shown in FIG. 4, the detector 310 of the detection system 300 according to at least one embodiment of the present disclosure may further include a first acting device 304. FIG. 6 is a schematic diagram of the force application operation performed by the first acting device 304 and the second acting device 305 which will be described below. As shown in FIG. 6, the first acting device 304 may include a movable first end 3041, and the first end 3041 is configured to apply a force to the first sealing cover 104 of the detection chip 301 mounted on the chip mounting structure 302 . For example, the first end 3041 can be installed at the end of the screw, and the screw can be driven by a screw pair. For example, the first end 3041 can be installed on a rod driven by a pneumatic or a cam, so that the first end 3041 can move in the vertical direction in the figure.

For example, in use, when a force is applied to the first sealing cover 104 through the first end 3041 of the first acting device 304, the first sealing cover 104 undergoes elastic deformation, and further passes through the first end 3041 of the first acting device 304. 3041 applies a force on the first sealing cover 104, and then applies force to the liquid in the liquid storage chamber of the liquid storage layer 101 (including the pre-stored liquid in the liquid storage chamber and the target liquid injected by the liquid injection device 303) to press, thereby The liquid in the liquid storage chamber of the liquid storage layer 101 flows through the damaged second pierceable sealing layer of the detection chip 301 and enters the through hole in the flow channel layer 105 of the detection chip 301, and then can enter the flow channel layer 105 In the liquid flow channel. It should be understood that since the first sealing cover 104 can be elastically deformed, after the first action device 304 is removed to release, the first sealing cover 104 can be restored to its original shape without being damaged, so that the liquid flow in the flow channel layer 105 At least a part of the liquid in the channel returns to the through hole of the flow channel layer 105, and can further return to the liquid storage chamber of the liquid storage layer 101 via the second pierceable sealing layer of the detection chip 301, which is destroyed.

In addition, it should be understood that the first acting device 304 may further include a driver (for example, a motor) for driving the movable first end 3041, so as to automate the operation of the detection system. However, the first action device 304 may not include a driver for driving the movable first end 3041, for example, the movable first end 3041 can be manually driven, which can also reduce the cost of the detection system. The embodiment of the present disclosure does not limit this.

It should be understood that, in some embodiments, the detection system 300 may not include the first acting device 304. For example, the first sealing cover 104 of the detection chip 301 mounted on the chip mounting structure 302 may be manually applied. The embodiment does not limit this.

As shown in FIG. 4, the detector 310 of the detection system 300 according to at least one embodiment of the present disclosure may further include a second acting device 305. As shown in FIG. 6, the second acting device 305 may include a movable second end 3051, and the second end 3051 is configured to apply a force to the second sealing cover 106 of the detection chip 301 mounted on the chip mounting structure 302 . Similarly, for example, the second end 3051 can be installed at the end of the screw, and the screw can be driven by a screw pair, and for example, the second end 3051 can be installed on a rod driven by pneumatic or cam, so that the first The two ends 3051 can move in the vertical direction in the figure.

For example, in use, when a force is applied to the second sealing cover 106 through the second end 3051 of the second acting device 305, the second sealing cover 106 is elastically deformed, and further passes through the second end 3051 of the second acting device 305. 3051 applies a force on the second sealing cover 106, and then applies a force to the second pierceable sealing layer of the detection chip 301, thereby destroying the second pierceable sealing layer of the detection chip 301, so that the storage of the detection chip 301 The liquid storage chamber of the liquid layer 101 communicates with the through holes of the flow channel layer 105 and the liquid flow channel in the flow channel layer 105. It should be understood that since the second sealing cover 106 can be elastically deformed, after the second action device 305 is removed, the second sealing cover 106 can be restored to its original shape without being damaged.

In addition, it should be understood that the second acting device 305 may further include a driver (for example, a motor) for driving the movable second end 3051, so as to automate the operation of the detection system. However, the second acting device 305 may not include a driver for driving the movable second end 3051, for example, the movable second end 3051 can be manually driven, which can also reduce the cost of the detection system 300. The embodiment of the present disclosure does not limit this.

In some embodiments, after the second pierceable sealing layer is destroyed by the second end 3051 of the second acting device 305, the liquid in the liquid storage chamber of the liquid storage layer 101 (including the pre-stored liquid in the liquid storage chamber and the passage The target liquid injected by the liquid injection device 303 can enter the through hole of the flow channel layer 105 through the destroyed second pierceable sealing layer. When a force is applied to the sealing cover 106, the second sealing cover 106 undergoes elastic deformation, and the second end 3051 of the second acting device 305 applies a force to the second sealing cover 106, and then to the through hole of the flow channel layer 105 The liquid inside exerts a force, so that the liquid in the through hole of the flow channel layer 105 enters the liquid flow channel in the flow channel layer 105 of the detection chip 301. It should be understood that since the second sealing cover 106 can be elastically deformed, after the second action device 305 is removed, the second sealing cover 106 can be restored to its original shape without being damaged, so that the liquid flow channel of the flow channel layer 105 At least a part of the liquid returns to at least one of the liquid storage chamber of the liquid storage layer 101 and the through hole of the flow channel layer 105.

It should be understood that, in some embodiments, the detection system 300 may not include the second action device 305. For example, the second sealing cover 106 of the detection chip 301 mounted on the chip mounting structure 302 may be manually applied. The embodiment does not limit this.

As shown in FIG. 4, the detector 310 of the detection system 300 according to at least one embodiment of the present disclosure may further include a gas operating device 306. FIG. 7 is a schematic diagram of the gas operation operation performed by the gas operation device 306. As shown in FIG. 7, the gas operating device 306 includes a gas channel 3061 and is configured to blow gas or inhale gas through the gas channel 3061. For example, the gas operating device 306 may include a blowing and suction fan, which is in communication with the gas channel 3061, so that gas can be blown or sucked in through the gas channel 3061. However, it should be understood that the gas operating device 306 may also include other devices such as a fan to blow gas or inhale gas through the gas channel 3061, which is not limited in the embodiment of the present disclosure.

For example, in use, the gas channel 3061 of the gas operating device 306 is in communication with one end of the liquid channel 1052 in the channel layer 105 of the detection chip 301. For example, the gas operating device 306 can extract the gas in the liquid flow channel 1052 through the gas channel 3061 to form a negative pressure in the liquid flow channel 1052, so that the liquid storage chamber of the liquid storage layer 101 and the through hole of the flow channel layer 105 The liquid in at least one of them flows through the damaged second pierceable sealing layer of the detection chip 301 and enters the liquid flow channel 1052 in the flow channel layer 105 of the detection chip 301. For example, the gas operating device 306 can blow gas to the liquid channel 1052 through the gas channel 3061 to form a positive pressure in the liquid channel 1052, so that at least a part of the liquid in the liquid channel 1052 of the channel layer 105 passes through the detection chip The damaged second pierceable sealing layer in 301 returns to at least one of the liquid storage chamber of the liquid storage layer 101 and the through hole of the flow channel layer 105. For example, the gas operating device 306 may be a gas pump, which may be mechanically driven or manually driven. For example, the gas passage 3061 may at least partially include a pipe, and the pipe may be a metal pipe or a plastic pipe.

In addition, in some embodiments, the first acting device 304 and the gas operating device 306 can also be used together to more efficiently make at least one of the liquid storage chamber of the liquid storage layer 101 and the through hole of the flow channel layer 105 The liquid enters the liquid flow channel of the flow channel layer 105 or causes the liquid in the liquid flow channel of the flow channel layer 105 to return to at least one of the liquid storage chamber of the liquid storage layer 101 and the through hole of the flow channel layer 105.

FIG. 8 is a flowchart of a method of operating a detection chip according to at least one embodiment of the present disclosure. This method can be applied to the detection chip according to any embodiment of the present disclosure. As shown in FIG. 8, a method 800 for operating a detection chip according to at least one embodiment of the present disclosure includes:

S810: Move the first sealing cover to pierce the first pierceable sealing layer to inject the target liquid;

S820, after injecting the target liquid, move the first sealing cover to seal at least the part where the first pierceable sealing layer overlaps the first opening;

S830, applying a force on the second sealing cover to pierce the second pierceable sealing layer so that the liquid flow channel is in liquid communication with the liquid storage chamber; and

S840. Drive the liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer, so that the liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer flows into the liquid flow channel or causes the liquid in the liquid flow channel to flow into the liquid flow channel. The liquid flows back into at least one of the through holes of the liquid storage chamber and the flow channel layer.

In some embodiments, step S840 may include:

Pressing at least one of the first sealing cover and the second sealing cover so that the liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer flows into the liquid flow channel; and

At least one of the first sealing cover and the second sealing cover is released, so that the liquid in the liquid flow channel flows back into at least one of the liquid storage chamber and the through hole of the flow channel layer.

In some embodiments, in step S840, at least one of the first sealing cover and the second sealing cover may be pressed so that the liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer is 0.1 nm/ The velocity of sec-100 m/sec (for example, 1 micron/sec-0.1 m/sec; such as 10 mm/sec) flows into the liquid flow channel, such as 0.1 nanometers to 10 cm (e.g., 0.1 nanometers, 0.5 nanometers, 1 nanometers). , 5 nm, 10 nm, 100 nm, 1 μm, 5 μm, 10 μm, 50 μm, 100 μm, 1 mm, 5 mm, 1 cm, 5 cm, 10 cm, etc.) to provide good mixing or dilution Effect.

In some embodiments, in step S840, at least one of the first sealing cover and the second sealing cover may be released, so that the liquid in the liquid flow channel is at a speed of 0.1 nanometers/second to 100 meters/second (for example, 1 The velocity of micron/sec-0.1 m/sec; for example, 10 mm/sec) flows back into at least one of the through holes of the liquid storage chamber and the flow channel layer to provide a good mixing or dilution effect.

In other embodiments, step S840 may include:

Extracting the gas in the liquid flow channel so that the liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer flows into the liquid flow channel; and

In some embodiments, in step S840, the gas in the liquid flow channel may be extracted, so that the liquid in at least one of the liquid storage chamber and the through hole of the flow channel layer is at a speed of 0.1 nanometers/second to 100 meters/second. (E.g., 1 micron/sec-0.1 m/sec; e.g. 10 mm/sec) flow into the liquid channel at a velocity of, for example, 0.1 nanometers to 10 cm (e.g., 0.1 nanometers, 0.5 nanometers, 1 nanometers, 5 nanometers, 10 nanometers, 100 Nanometer, 1 micron, 5 micron, 10 micron, 50 micron, 100 micron, 1 mm, 5 mm, 1 cm, 5 cm, 10 cm, etc.) to provide a good mixing or dilution effect.

In some embodiments, in step S840, gas may be injected into the liquid flow channel, so that the liquid in the liquid flow channel moves at a speed of 0.1 nanometer/sec-100 m/sec (for example, 1 micron/sec-0.1 m/sec). ; For example, 10 mm/sec) flow back into at least one of the through holes of the liquid storage chamber and the flow channel layer to provide a good mixing or dilution effect.

In some embodiments, the method 800 may include repeating step S840 multiple times.

The number of times to repeat step S840 may be determined based on experience, or may be preset, or determined through observation, which is not limited in the embodiment of the present disclosure. For example, the number of times of repeating step S840 can be preset to 30 times to provide a good mixing or dilution effect.

In some embodiments, after step S840 is repeated multiple times, the method 800 may further include:

Pressing at least one of the first sealing cover and the second sealing cover, so that the liquid in at least one of the liquid storage chamber and the through hole of the flow channel layer flows into the liquid flow channel. In this way, the mixed or diluted liquid can be transported to other locations of the detection chip or other detection devices or analysis devices connected to the detection chip through the liquid flow channel for detection, analysis, reaction, diagnosis or collection, etc. Follow-up operations.

The following points need to be explained:

(1) In the drawings of the embodiments of the present disclosure, only the structures related to the embodiments of the present disclosure are involved, and other structures can refer to the usual design.

(2) For clarity, in the drawings used to describe the embodiments of the present disclosure, the thickness and size of layers or structures are exaggerated. It will be understood that when an element such as a layer, film, region, or substrate is referred to as being "on" or "under" another element, the element can be "directly" on or "under" the other element. Or there may be intermediate elements.

(3) In the case of no conflict, the features of the same embodiment and different embodiments of the present disclosure can be combined with each other.

The above are only specific implementations of the present disclosure, but the protection scope of the present disclosure is not limited thereto, and the protection scope of the present disclosure should be subject to the protection scope of the claims.

Claims

A detection chip includes:

The liquid storage layer has opposite first and second surfaces, and includes a liquid storage chamber, wherein the liquid storage chamber has a first opening on the first surface and a second opening on the second surface ；

A first pierceable sealing layer to seal the first opening of the liquid storage chamber;

A second pierceable sealing layer to seal the second opening of the liquid storage chamber;

The first sealing cover is located on the side of the first pierceable sealing layer away from the liquid storage layer, and is movably arranged to expose or seal at least the first pierceable sealing layer and the first The overlapping part of the opening;

The flow channel layer is located on the side of the second pierceable sealing layer away from the liquid storage layer, and includes a through hole and a liquid flow channel communicating with the through hole, wherein the through hole exposes at least the The part where the second pierceable sealing layer overlaps with the second opening; and

The second sealing cover is located on the side of the flow channel layer away from the liquid storage layer, and seals the opening of the through hole on the surface of the flow channel layer away from the liquid storage layer.
8. The detection chip of claim 1, wherein the first sealing cover can be elastically deformed.
The detection chip according to claim 1 or 2, wherein the second sealing cover can be elastically deformed, and within the elastic deformation range of the second sealing cover, an external force perpendicular to the second sealing cover is allowed When acting on the second sealing cover, it can further act on the second pierceable sealing layer to destroy the second pierceable sealing layer.
The detection chip according to any one of claims 1 to 3, wherein, in the case that the first sealing cover seals at least the overlapping portion of the first pierceable sealing layer and the first opening, the first sealing cover The orthographic projection of a sealing cover on the liquid storage layer at least partially overlaps the first opening.
The detection chip according to any one of claims 1 to 4, wherein the liquid flow channel of the flow channel layer at least partially includes a hollow area in the flow channel layer.
The detection chip according to any one of claims 1 to 5, further comprising an adhesive layer,

Wherein, the adhesive layer is between the second sealing cover and the flow channel layer to connect the second sealing cover and the flow channel layer.
The detection chip according to claim 6, wherein:

The adhesive layer includes a hollow area, and

The opening of the through hole on the surface of the flow channel layer away from the liquid storage layer is within the shape of the orthographic projection of the hollow area on the flow channel layer.
7. The detection chip according to any one of claims 1-7, wherein the liquid storage chamber comprises a through hole in the liquid storage layer.
A detection system includes:

Detector, including chip mounting structure; and

The detection chip according to any one of claims 1-8,

Wherein, the chip mounting structure is used for mounting the detection chip.
9. The detection system according to claim 9, wherein the detection instrument further comprises a liquid injection device,

Wherein, the liquid injection device includes a movable end and a liquid injection structure,

The movable end portion is configured to pierce the first pierceable sealing layer when the detection chip is mounted on the chip mounting structure, and

The liquid injection structure is configured to inject a target liquid into the liquid storage chamber.
The detection system according to claim 9 or 10, wherein the detection instrument further comprises a first action device,

Wherein, the first acting device includes a movable first end,

The first end portion is configured to apply a force to the first sealing cover of the detection chip mounted on the chip mounting structure.
The detection system according to any one of claims 9-11, wherein the detection instrument further comprises a second action device,

Wherein, the second acting device includes a movable second end,

The second end portion is configured to apply a force to the second sealing cover of the detection chip mounted on the chip mounting structure.
The detection system according to any one of claims 9-12, wherein the detector further comprises a gas operating device,

Wherein, the gas operating device includes a gas channel, and is configured to blow gas or inhale gas through the gas channel.
A method of operating the detection chip according to any one of claims 1-8, comprising:

Move the first sealing cover to pierce the first pierceable sealing layer to inject the target liquid;

After injecting the target liquid, moving the first sealing cover to seal at least the overlapping portion of the first pierceable sealing layer and the first opening;

Applying a force on the second sealing cover to pierce the second pierceable sealing layer, so that the liquid flow channel is in liquid communication with the liquid storage chamber; and

The liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer is driven so that the liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer Flow into the liquid flow channel or cause the liquid in the liquid flow channel to flow back into at least one of the liquid storage chamber and the through hole of the flow channel layer.
The method of claim 14, wherein the liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer is driven so that the liquid in the liquid storage chamber and the flow channel layer The liquid in at least one of the through holes flows into the liquid flow channel or causes the liquid in the liquid flow channel to flow back into at least one of the liquid storage chamber and the through holes of the flow channel layer, include:

Press at least one of the first sealing cover and the second sealing cover so that the liquid in at least one of the liquid storage chamber and the through hole of the flow channel layer flows into the liquid flow channel ;as well as

At least one of the first sealing cover and the second sealing cover is released, so that the liquid in the liquid flow channel flows back into the liquid storage chamber and the through hole of the flow channel layer at least Within one.
The method of claim 14, wherein the liquid in at least one of the through holes of the liquid storage chamber and the flow channel layer is driven so that the liquid in the liquid storage chamber and the flow channel layer The liquid in at least one of the through holes flows into the liquid flow channel or causes the liquid in the liquid flow channel to flow back into at least one of the liquid storage chamber and the through holes of the flow channel layer, include:

Extracting the gas in the liquid flow channel so that the liquid in at least one of the liquid storage chamber and the through hole of the flow channel layer flows into the liquid flow channel; and

Gas is injected into the liquid flow channel, so that the liquid in the liquid flow channel flows back into at least one of the liquid storage chamber and the through hole of the flow channel layer.
The method according to any one of claims 14 to 16, further comprising:

Press at least one of the first sealing cover and the second sealing cover so that the liquid in at least one of the liquid storage chamber and the through hole of the flow channel layer flows into the liquid flow channel .