WO2021129895A3 - Infectious plant rhabdovirus vector and method for non-transgenic, site-directed editing of plant genome - Google Patents

Infectious plant rhabdovirus vector and method for non-transgenic, site-directed editing of plant genome Download PDF

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Publication number
WO2021129895A3
WO2021129895A3 PCT/CN2021/077497 CN2021077497W WO2021129895A3 WO 2021129895 A3 WO2021129895 A3 WO 2021129895A3 CN 2021077497 W CN2021077497 W CN 2021077497W WO 2021129895 A3 WO2021129895 A3 WO 2021129895A3
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WO
WIPO (PCT)
Prior art keywords
plant
site
transgenic
infectious
genome
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PCT/CN2021/077497
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French (fr)
Chinese (zh)
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WO2021129895A2 (en
Inventor
李正和
马晓楠
王硕
彭幸幸
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浙江大学
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Application filed by 浙江大学 filed Critical 浙江大学
Priority to CN202180007363.6A priority Critical patent/CN115397997A/en
Priority to GB2210802.1A priority patent/GB2607743A/en
Publication of WO2021129895A2 publication Critical patent/WO2021129895A2/en
Publication of WO2021129895A3 publication Critical patent/WO2021129895A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Provided are a method for using a plant rhabdovirus vector to perform site-directed modification on plant chromosomal DNA, and a viral vector composition. Also provided is a method for performing editing on plant genome target DNA, said method not requiring a selectable marker gene, and comprising the following steps: using a plant rhabdovirus vector system to infect a plant and express a sequence-specific nuclease, the sequence-specific nuclease targeting a genome target site of the infected plant and cleaving the target site, and a DNA repair mechanism of the plant completing site-directed editing of the target site.
PCT/CN2021/077497 2019-12-23 2021-02-23 Infectious plant rhabdovirus vector and method for non-transgenic, site-directed editing of plant genome WO2021129895A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202180007363.6A CN115397997A (en) 2020-12-23 2021-02-23 Infectious plant rhabdovirus vector and method for site-specific editing of transgene-free plant genome
GB2210802.1A GB2607743A (en) 2019-12-23 2021-02-23 Infectious plant rhabdovirus vector and method for non-transgenic, site-directed editing of plant genome

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201911341221 2019-12-23
CN201911341221.4 2019-12-23
CN202011541902 2020-12-23
CN202011541902.8 2020-12-23

Publications (2)

Publication Number Publication Date
WO2021129895A2 WO2021129895A2 (en) 2021-07-01
WO2021129895A3 true WO2021129895A3 (en) 2021-08-19

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PCT/CN2021/077497 WO2021129895A2 (en) 2019-12-23 2021-02-23 Infectious plant rhabdovirus vector and method for non-transgenic, site-directed editing of plant genome

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3230629A1 (en) * 2021-09-08 2023-03-16 Beam Therapeutics Inc. Viral guide rna delivery

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104962580A (en) * 2015-06-30 2015-10-07 浙江大学 Recombinant plant rhabdovirus vector and construction method thereof
CN110511955A (en) * 2018-05-21 2019-11-29 中国农业大学 Plant cytoplasm rhabdovirus infectious clone, expression vector and its construction method and application

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104962580A (en) * 2015-06-30 2015-10-07 浙江大学 Recombinant plant rhabdovirus vector and construction method thereof
CN110511955A (en) * 2018-05-21 2019-11-29 中国农业大学 Plant cytoplasm rhabdovirus infectious clone, expression vector and its construction method and application

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GAO QIANG, XU WEN‐YA, YAN TENG, FANG XIAO‐DONG, CAO QING, ZHANG ZHEN‐JIA, DING ZHI‐HANG, WANG YING, WANG XIAN‐BING: "Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper and cereal genomic studies", NEW PHYTOLOGIST, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 223, no. 4, 1 September 2019 (2019-09-01), GB, pages 2120 - 2133, XP055834996, ISSN: 0028-646X, DOI: 10.1111/nph.15889 *

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WO2021129895A2 (en) 2021-07-01

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