WO2021114088A1 - 可变体积细胞培养袋 - Google Patents

可变体积细胞培养袋 Download PDF

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WO2021114088A1
WO2021114088A1 PCT/CN2019/124302 CN2019124302W WO2021114088A1 WO 2021114088 A1 WO2021114088 A1 WO 2021114088A1 CN 2019124302 W CN2019124302 W CN 2019124302W WO 2021114088 A1 WO2021114088 A1 WO 2021114088A1
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cell culture
pressing rod
variable volume
volume cell
culture bag
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PCT/CN2019/124302
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English (en)
French (fr)
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张豪
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苏州生动细胞生物科技有限公司
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Priority to PCT/CN2019/124302 priority Critical patent/WO2021114088A1/zh
Publication of WO2021114088A1 publication Critical patent/WO2021114088A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/02—Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions

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  • This application relates to the field of cell culture technology, and in particular to a variable volume cell culture bag.
  • Disposable bioreactor is a common equipment used for cell bag culture, such as GE Life Sciences WAVE bioreactor.
  • the disposable bioreactor is equipped with a tray device, which is used to contain the cell culture bag. Specifically, the cell culture bag is placed on the tray, and the tray is equipped with a heating device, and the bottom surface of the cell culture bag is in contact with the heating device.
  • the tray device of the disposable bioreactor is also equipped with a non-contact temperature probe.
  • the non-contact temperature probe collects the temperature of the bottom surface of the cell culture bag to control the temperature of the cell solution in the cell culture bag.
  • the bottom surface of the cell culture bag is generally welded to the pH and DO connectors by hot pressing, and the optical sensors on the pH and DO connectors will collect the pH and DO values of the cell solution in real time.
  • the commonly used cell culture bags are 1L, 2L, 5L, 10L, 20L, 50L, 100L, 200L, 500L, 1000L and other different specifications, but the minimum and maximum culture volume are determined by the area of the bottom of the bag and the height of the bag after bulging. It is not adjustable. In the same cell culture bag volume, pouring different volumes of cell solution will not only bring greater difficulties to the collection and control of temperature, pH and DO values, but also bring greater impact on the cell culture effect. The big impact, in the actual operation process, there are the following deficiencies:
  • the purpose of this application is to overcome the defects that the fixed volume cell culture bag in the prior art is not conducive to the collection and control of temperature, pH and DO values during the culture process, and affects the cell proliferation speed, and to provide a variable volume cell culture bag , Not only meets the physiological environment requirements for cell growth under the condition of less cell solution as the initial amount, and provides a physical environment for accurate collection of temperature, pH and DO values, but also prolongs the growth of cells in the culture bag Process and time, thereby reducing manual operation time and the risk of contamination.
  • variable volume cell culture bag provided by the present application includes a closed bag body provided with a plurality of interfaces for liquid or gas to enter and exit, and at least one side of the closed bag body is provided A detachable partition clip is used to separate the closed bag body into two independent spaces.
  • the closed bag body is formed by processing and combining the upper bag piece and the lower bag piece into a closed bag cavity, which is separated and clamped on the bag body in a freely movable position to control the size of the space in the bag.
  • each of the partition clips is arranged on the same side of the plurality of interfaces.
  • the partition clamp includes an upper pressing rod and a lower pressing rod, one end of the upper pressing rod and one end of the lower pressing rod are hinged by a rotating shaft, and the other ends of the two are connected by a knob bolt, so as to adjust the clamping tightness. degree.
  • the partition clamp includes an upper pressing rod and a lower pressing rod, one end of the upper pressing rod and one end of the lower pressing rod are hinged by a rotating shaft, and the other ends of the two are nested on the stud through a through hole.
  • Nail heads are arranged at both ends of the nail to limit the position, and a compression spring is arranged between the upper pressure rod and/or the lower pressure rod and the nail head.
  • the width position L of the partition clip (10) at the closed bag body is confirmed by the following formula
  • V represents the volume of water in the closed bag
  • ⁇ represents the inclination angle of the shaker
  • the top surface of the closed bag body is provided with an air outlet, an air inlet, a sampling port, a liquid input port, and a collection port, all of which are provided with a sterile quick connector.
  • a gas shut-off valve is provided at the gas outlet, and when the gas pressure in the bag reaches a certain threshold, the excess gas will be automatically discharged out of the bag.
  • At least two induction joints are provided on the bottom surface of the closed bag body.
  • two opposite sides of the closed bag body are provided with mounting rods for mounting on the bioreactor.
  • the closed bag body there are at least two heat-pressing edges on the side adjacent to the mounting rod. Two hot-pressed edges increase the rigidity of both sides and avoid large wrinkles after inflation.
  • variable volume cell culture bag can provide a condition for cell culture with less cell solution as the initial amount of cell culture, thereby avoiding excessive medium osmotic pressure and cell wrinkles caused by too fast evaporation of cell solution. Shrinkage, and even cause cell dysfunction, and eventually cause cell apoptosis.
  • variable volume cell culture bag can meet the precise collection of culture parameters such as temperature, pH, and DO with less cell solution as the initial amount of cell culture.
  • Figure 1 is a schematic diagram of the top structure of a variable volume cell culture bag.
  • Figure 2 is a schematic diagram of the bottom structure of a variable volume cell culture bag.
  • Fig. 3 is a schematic structural diagram of an embodiment of the partition clip in Fig. 1.
  • Figure 4-1 is a schematic diagram of the state of use of the variable volume cell culture bag in Figure 1.
  • Fig. 4-2 is a schematic diagram of calculating the position of the dividing clip of the variable volume cell culture bag in Fig. 1.
  • Fig. 5 is a diagram of the use state of the variable volume cell culture bag in Fig. 1.
  • Fig. 6 is a schematic structural diagram of another embodiment of the partition clip in Fig. 1. .
  • variable-volume cell culture bag shown in Figures 1 to 3 the variable-volume cell culture bag, includes a closed bag body 1, which is provided with multiple interfaces for liquid or gas in and out, and the closed bag A detachable partition clip 10 is provided on one side of the body 1 to divide the closed bag body 1 into two independent spaces.
  • the partition clip 10 is arranged on the same side of the multiple interfaces.
  • the multiple partition clips 10 When there are multiple partition clips 10 on the closed bag body 1, they can be located on the same side, or they can be separately arranged on different sides of the closed bag body 1, but the multiple partition clips 10 should be provided on the same side of the multiple interfaces ( For example, divider clips 10 are arranged around these interfaces, or divider clips are arranged side by side on the same side of multiple interfaces), that is, the divider clip 10 cannot be arranged between multiple interfaces.
  • the material of the partition clip 10 is a metal material.
  • the main body is made of SUS304 stainless steel or 6061T aluminum alloy, which increases the rigidity and strength of the partition clip 10, which is an up-and-down opening and closing structure.
  • the divider clip 10 includes an upper pressing rod 12 and a lower pressing rod 13. One end of the upper pressing rod 12 and one end of the lower pressing rod 13 are hinged by a rotating shaft 15, and the other end of the two is connected by a knob bolt 14 to realize adjustment. Clamping tightness. An elastic rubber layer 11 is also provided at the joint of the upper pressing rod 12 and the lower pressing rod 13.
  • this application provides a diagram of the use state of a variable volume cell culture bag.
  • the heating plate B In the normal state, the heating plate B is in a horizontal position and is located below the culture bag D. The probe position of the center of the heating plate It is located at A.
  • the shaker When in use, the shaker forms an inclination angle ⁇ with the horizontal position.
  • the swing angle of the shaker is determined, the following formula can be used in this application to count the minimum culture obtained when the separator is placed in different positions volume:
  • A the position of the probe in the center of the heating plate
  • V The volume of water in the closed bag
  • variable volume culture bag can obtain a wider minimum and maximum culture volume.
  • the minimum culture volume obtained by adjusting the partition clamp is proportional to the area of the bottom of the culture bag, and the culture medium must cover the temperature probe at the center of the tray to obtain the best partition clamp position for controlling the temperature.
  • the top surface of the closed bag body 1 is provided with an air outlet 3, an air inlet 4, a sampling port 5, a liquid input port 6, and a collection port 7. There are sterile quick connectors on each inlet and outlet.
  • the gas outlet 3 is also provided with a gas shut-off valve 16.
  • the inductive connector 8 includes at least two inductive connectors. As shown in FIG. 2, there are two inductive connectors, which are respectively a connector for connecting a pH sensor probe and a connector for connecting a DO sensor probe.
  • the two opposite sides of the closed bag body 1 are provided with mounting rods 2 for mounting on the bioreactor.
  • the mounting rod 2 is a glass fiber rod.
  • the two blank holders increase the rigidity of both sides and avoid large wrinkles after inflation.
  • the closed bag body 1 is a flexible bag body, and the material used is a multilayer transparent biocompatible inert material.
  • the inner layer that is, the liquid contact layer, is a low-density polyethylene (LDPE) layer.
  • LDPE low-density polyethylene
  • the basic structure of the variable-volume cell culture bag in this embodiment is the same as that in Embodiment 1, except that the partition clip 10 includes an upper pressure rod 12 and a lower pressure rod 13, and the upper pressure rod 12 One end of the stud and one end of the lower pressing rod 13 are hinged by a rotating shaft 15, and the other end of the two is nested on the stud 17 through a through hole.
  • the two ends of the stud are provided with nail heads 18 to limit the position, and the upper pressure rod and/or the lower pressure
  • a compression spring 19 is provided between the rod and the nail head.
  • the elastic force of the pressure spring 19 makes the upper pressure rod 12 and the lower pressure rod 13 bite together, and the upper pressure rod 12 and the lower pressure rod 13 can be easily separated by pressing the pressure spring 19, thereby realizing the separation clip 10 clamping and opening and closing functions.
  • variable volume cell culture bag The specific usage of the variable volume cell culture bag of this application is as follows:
  • Example 1 when the cell culture bag is not inflated, the knob bolt 14 is twisted, the upper pressing rod 12 and the lower pressing rod 13 are opened, and the closed bag body 1 is placed on the upper pressure. Between the rod 12 and the lower pressing rod 13, then close the upper pressing rod 12 and the lower pressing rod 13. After the interference fit of the elastic rubber layer 11 and the fixing of the knob bolt 14, the closed bag body 1 is divided into two parts, one part It is used to input gas and liquid for cell culture, and the other part is partitioned by the partition clip 10 to avoid the entry of gas and liquid. Moving the position of the partition clip 10 can gradually increase the cell culture volume.
  • the osmotic pressure of the medium is too high, the cells shrink, and even cause cell dysfunction, which eventually leads to cell apoptosis; on the other hand, by changing the volume of the cell culture bag, it can be satisfied that less cell solution is used as the initial amount of cell culture Accurate collection of culture parameters such as temperature, pH and DO.
  • a gas shut-off valve 16 on the air outlet 3 of the cell culture bag. As shown in Figure 5, the valve value of the gas shut-off valve 16 is 0.075 psi.
  • the gas pressure in the bag When the gas pressure in the bag is greater than this threshold, the excess gas in the bag will It will be automatically discharged to the outside of the bag. In the state of dynamic equilibrium, the gas pressure in the bag is maintained at about 0.075psi. Because the gas pressure in the bag is too small, it is not enough to move the position of the divider clip 10.

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Abstract

一种可变体积细胞培养袋，它包括封闭袋体，所述封闭袋体上设有容液体或气体进出的多个接口，所述封闭袋体的一侧设有可拆卸的分隔夹，用来将所述封闭袋体分隔成两个独立的空间。

Description

可变体积细胞培养袋 技术领域
本申请涉及细胞培养技术领域，尤其涉及一种可变体积细胞培养袋。
背景技术
一次性生物反应器是用作细胞袋式培养的一种常用设备，如GE生命科学WAVE生物反应器。一次性生物反应器带有托盘装置，托盘装置用于容纳细胞培养袋，具体地，细胞培养袋放置于托盘上，托盘上带有加热装置，细胞培养袋底面与加热装置接触。同时，一次性生物反应器的托盘装置上也带有非接触式温度探头，非接触式温度探头通过采集细胞培养袋底部袋面的温度来控制细胞培养袋内细胞溶液的温度。另外，细胞培养袋底面一般会采用热压的方式焊接pH和DO接头，pH和DO接头上的光学传感器会实时采集细胞溶液的pH和DO数值。
目前常用的细胞培养袋为1L、2L、5L、10L、20L、50L、100L、200L、500L、1000L等不同规格，但其最小和最大培养体积均由袋底面积和袋鼓起后高度决定，不可调节，在相同的细胞培养袋体积下，灌入不同体积的细胞溶液，不但会对温度、pH和DO数值的采集以及控制带来较大的困难，而且也会对细胞培养效果带来较大的影响，在实际操作过程中具体存在以下不足：
一方面，较少细胞溶液作为细胞培养的初始量平铺在较大体积的细胞培养袋内会造成细胞溶液较快的蒸发，会导致培养基渗透压过高，导致细胞皱缩，甚至引起细胞功能紊乱，最终引起细胞凋亡。特别地，无法有效覆盖与非接触式温度探头接触区域的细胞培养袋底面的面积，这就无法精确控制细胞培养袋内的细胞培养液的温度。由于不能有效接触细胞溶液，pH和DO光学传感器也不能实时精确采集数值，因为得不到精确的数据反馈，也就不能正确的采取补 液的动作，从而起不到实时调整细胞溶液pH和DO的目的。这种以较少细胞溶液为出初始量平铺在较大体积的细胞培养袋内的方式，不但在生理环境上会对细胞生长带来影响，而且对温度、pH和DO数值采集的不精确性也会极大的影响细胞生长的条件。
另一方面，较多细胞溶液作为细胞培养的初始量会带来细胞密度过低，扩增速度慢的状况，在体外，细胞的对数增殖需要细胞之间的相互作用，当细胞密度较低时，细胞增殖速度明显降低。另外，较多细胞溶液作为细胞培养的初始量不但带来了成本的浪费，而且也要求从病人体内采集更多的血液，也是不可取的。
发明内容
本申请的目的在于克服现有技术中固定体积的细胞培养袋在培养过程中不利于温度、pH和DO数值的采集及控制，且影响细胞增殖速度的缺陷，提供一种可变体积细胞培养袋，不但满足了以较少细胞溶液为初始量条件下细胞自身生长的生理环境要求，以及提供了对温度、pH和DO数值的精确采集的物理环境，而且也可以延长细胞在培养袋内的生长过程和时间,从而减少人工的操作时间以及被污染风险。
为实现上述目的，本申请提供的可变体积细胞培养袋，它包括封闭袋体，所述封闭袋体上设有容液体或气体进出的多个接口，所述封闭袋体的至少一侧设有可拆卸的分隔夹，用来将所述封闭袋体分隔成两个独立的空间。所述封闭袋体是由上袋片和下袋片经过加工组合成一个封闭袋腔，分隔夹在袋体上可自由移动位置来控制袋内空间的大小。
可选地，每个所述分隔夹均设置于所述多个接口的同一侧。
可选地，所述分隔夹包括上压杆和下压杆，所述上压杆的一端和下压杆的一端通过旋转轴铰接，两者另一端通过旋钮螺栓相连，以实现调节夹持松紧程度。
可选地，所述分隔夹包括上压杆和下压杆，所述上压杆的一端 和下压杆的一端通过旋转轴铰接，两者另一端通过通孔嵌套在栓钉上，栓钉两头设有钉头来限位，上压杆和/或下压杆与钉头之间设有压簧。
可选地，所述分隔夹(10)在所述封闭袋体的宽度位置L通过以下公式确认
Figure PCTCN2019124302-appb-000001
其中，V表示所述封闭袋体中水的体积，θ表示摇床倾角。
可选地，所述封闭袋体的顶面设有出气口、进气口、取样口、液体输入口、收集口，口上均设有无菌快接头。
可选地，所述出气口处设置有气体截止阀，当袋内气体压力达到一定阀值，多余气体会自动排出袋外。
可选地，所述封闭袋体的底面至少设置有两个感应接头。
可选地，所述封闭袋体的两对向侧边设置有用于安装在生物反应器上的安装杆。
可选地，所述封闭袋体上，与所述安装杆相邻的侧边至少有两条热压封边。两条热压封边增加了两侧边的刚度，避免充气后出现较大褶皱。
本申请的有益效果：
1)采用可变体积细胞培养袋，可以提供了一个以较少细胞溶液作为细胞培养初始量进行细胞培养的条件，从而避免了细胞溶液蒸发过快所导致的培养基渗透压过高、细胞皱缩，甚至引起细胞功能紊乱，最终引起细胞凋亡的情况。
2)采用可变体积细胞培养袋，可以满足在较少细胞溶液作为细胞培养初始量下对温度、pH和DO等培养参数的精确采集。
附图说明
图1为可变体积细胞培养袋的顶面结构示意图。
图2为可变体积细胞培养袋的底面结构示意图。
图3为图1中分隔夹的一种实施方式的结构示意图。
图4-1为图1中可变体积细胞培养袋的使用状态示意图。
图4-2为图1中可变体积细胞培养袋的分割夹位置计算示意图。
图5为图1中可变体积细胞培养袋的使用状态图。
图6为图1中分隔夹的另一种实施方式的结构示意图。。
其中，1、封闭袋体；2、安装杆；3、出气口；4、进气口；5、取样口；6、液体输入口；7、收集口；8、感应接头；9、热压封边；10、分隔夹；11、弹性橡胶层；12、上压杆；13、下压杆；14、旋钮螺栓；15、旋转轴；16、气体截止阀；17、栓钉；18、钉头；19、压簧。
具体实施方式
以下结合附图和具体实施例对本申请作进一步的详细描述。
实施例1
如图1～3所示的可变体积细胞培养袋，可变体积细胞培养袋，它包括封闭袋体1，所述封闭袋体1上设有容液体或气体进出的多个接口，封闭袋体1的一侧设有可拆卸的分隔夹10，用来将所述封闭袋体1分隔成两个独立的空间。分隔夹10设置于多个接口的同一侧。当封闭袋体1上有多个分隔夹10时，它们可以位于同一侧边，也可以分设在封闭袋体1的不同侧边，但多个分隔夹10应设置于多个接口的同一侧(如围绕这些接口周围设置分隔夹10，或者把分隔夹并排布置在多个接口同一侧)，就是说，不可以将分隔夹10设置在多个接口之间。
本实施例中分隔夹10材料为金属材料。主体材料为SUS304不锈钢或6061T铝合金，增加了分隔夹10的刚度与强度，其为一个上下开合结构。所述分隔夹10包括上压杆12和下压杆13，所述上压杆12的一端和下压杆13的一端通过旋转轴15铰接，两者另一端通过旋钮螺栓14相连，以实现调节夹持松紧程度。上压杆12和下压杆13的咬合处还设有弹性橡胶层11。
如图4-1、4-2所示，本申请提供了一种可变体积细胞培养袋的使用状态图，正常状态加热板B处于水平位置，且位于培养袋D 下方，加热板中心探头位置位于A处，当在使用状态时，摇床与水平位置成倾斜角θ，当摇床摇摆倾角确定时，本申请可通过下列公式，即可计数出分隔夹放置不同位置时，所得的最小培养体积：
V＝1/2*1/3*π*R 2*H    (公式1)
其中：tanθ＝R/H   (公式2)
Figure PCTCN2019124302-appb-000002
Figure PCTCN2019124302-appb-000003
其中：A—加热板中心探头位置；
θ—摇床倾角；
L—分割夹所在封闭袋体上的宽度位置；
R—圆半径；
H—圆锥高度；
V—封闭袋体中水的体积；
可变体积培养袋，可以获得更广的最小和最大培养体积。通过调整分隔夹获取最小培养体积，跟培养袋底面积成正比，且培养液必须覆盖住托盘中心温度探头，才能获取控制好温度的最佳分隔夹位置。
所述封闭袋体1的顶面设有出气口3、进气口4、取样口5、液体输入口6、收集口7。各个进出口上均设有无菌快接头。所述出气口3处还设置有气体截止阀16。
所述封闭袋体1的底面至少设置有两个感应接头8。可用于接通传感器以监测细胞培养袋内的相关指标。所述感应接头8至少包括两个，图2中所示的是2个感应接头，分别是用于接入pH值传感器探头的接头和用于接DO的传感器探头的接头。
所述封闭袋体1的两对向侧边设置有用于安装在生物反应器上的安装杆2。本实施例安装杆2为玻璃纤维棒。
所述封闭袋体1上，与所述安装杆相邻的侧边至少有两条热压封边9。两条压边增加了两侧边的刚度,避免充气后出现较大褶皱。
所述的封闭袋体1为柔性袋体，所用材料为多层透明生物相容性惰性材料，一般内层，即接触液体层，为低密度聚乙烯(LDPE)层。封闭袋体1由上袋片和下袋片经过加工组合成一个封闭袋腔。
实施例2
如图6所示，本实施例中可变体积细胞培养袋的基本结构与实施例1相同，区别在于：所述分隔夹10包括上压杆12和下压杆13，所述上压杆12的一端和下压杆13的一端通过旋转轴15铰接，两者另一端通过通孔嵌套在栓钉17上，栓钉两头设有钉头18来限位，上压杆和/或下压杆与钉头之间设有压簧19。在不受力状态下，压簧19的弹力使得上压杆12和下压杆13咬合在一起，通过按压压簧19可以轻松使上压杆12和下压杆13的分开，从而实现分隔夹10的夹紧及开闭功能。
本申请的可变体积细胞培养袋具体用法如下：
以实施例1来说，如图3、4所示，在细胞培养袋未充气时，扭开旋钮螺栓14，上压杆12、下压杆13张开，将封闭袋体1放置于上压杆12和下压杆13之间，然后闭合上压杆12和下压杆13，经过弹性橡胶层11的过盈配合和旋钮螺栓14的固定，将封闭袋体1分为两个部分，一部分用于输入气体和液体进行细胞培养，另一部分则被分隔夹10隔断，避免了气体和液体的进入，移动分隔夹10的位置，可以逐步增大细胞培养体积。这种通过改变分隔夹10位置来改变细胞培养袋体积的方法，一方面，提供了一个以较少细胞溶液作为细胞培养初始量进行细胞培养的条件，从而避免了细胞溶液蒸发过快所导致的培养基渗透压过高、细胞皱缩，甚至引起细胞功能紊乱，最终引起细胞凋亡的情况；另一方面，通过改变细胞培养袋的体积，可以满足在较少细胞溶液作为细胞培养初始量下对 温度、pH和DO等培养参数的精确采集。另外，细胞培养袋出气口3上带有气体截止阀16,如图5所示，气体截止阀16的阀值为0.075psi,当袋内的气体压力大于这个阀值时，袋内多余气体将会自动排出到袋外，在动平衡状态下，袋内气体压力维持在0.075psi左右，由于袋内气体压力过小，不足以移动分隔夹10的位置。

Claims (10)

  1. 一种可变体积细胞培养袋，其特征在于，包括封闭袋体(1)，所述封闭袋体(1)上设有容液体或气体进出的多个接口，其特征在于：所述封闭袋体(1)的至少一侧设有可拆卸的分隔夹(10)，用来将所述封闭袋体(1)分隔成两个独立的空间。
  2. 根据权利要求1所述可变体积细胞培养袋，其特征在于：每个所述分隔夹(10)均设置于所述多个接口的同一侧。
  3. 根据权利要求2所述可变体积细胞培养袋，其特征在于：所述分隔夹(10)包括上压杆(12)和下压杆(13)，所述上压杆(12)的一端和下压杆(13)的一端通过旋转轴(15)铰接，两者另一端通过旋钮螺栓(14)相连，以实现调节夹持松紧程度。
  4. 根据权利要求2所述可变体积细胞培养袋，其特征在于：所述分隔夹(10)包括上压杆(12)和下压杆(13)，所述上压杆(12)的一端和下压杆(13)的一端通过旋转轴(15)铰接，两者另一端通过通孔嵌套在栓钉(17)上，栓钉两头设有钉头(18)来限位，上压杆和/或下压杆与钉头之间设有压簧(19)。
  5. 根据权利要求3或4所述可变体积细胞培养袋，其特征在于：所述分隔夹(10)在所述封闭袋体(1)的宽度位置L通过以下公式确认
    Figure PCTCN2019124302-appb-100001
    其中，V表示所述封闭袋体中水的体积，θ表示摇床倾角。
  6. 根据权利要求1所述可变体积细胞培养袋，其特征在于：所述封闭袋体(1)的顶面设有出气口(3)、进气口(4)、取样口(5)、液体输入口(6)、收集口(7)。
  7. 根据权利要求5所述可变体积细胞培养袋，其特征在于：所述出气口(3)处设置有气体截止阀(16)。
  8. 根据权利要求1所述可变体积细胞培养袋，其特征在于：所述封闭袋体(1)的底面至少设置有两个感应接头(8)。
  9. 根据权利要求1所述可变体积细胞培养袋，其特征在于： 所述封闭袋体(1)的两对向侧边设置有用于安装在生物反应器上的安装杆(2)。
  10. 根据权利要求1所述可变体积细胞培养袋，其特征在于：所述封闭袋体(1)上，与所述安装杆相邻的侧边至少有两条热压封边(9)。
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