WO2021049927A1 - Fortificante vegetal a base de micorrizas vesículo arbusculares, extractos y nutrientes vegetales - Google Patents
Fortificante vegetal a base de micorrizas vesículo arbusculares, extractos y nutrientes vegetales Download PDFInfo
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- WO2021049927A1 WO2021049927A1 PCT/MX2020/050014 MX2020050014W WO2021049927A1 WO 2021049927 A1 WO2021049927 A1 WO 2021049927A1 MX 2020050014 W MX2020050014 W MX 2020050014W WO 2021049927 A1 WO2021049927 A1 WO 2021049927A1
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
Definitions
- Vegetable fortifier based on arbuscular vesicle mycorrhizae, extracts and plant nutrients.
- the product improves the efficiency in the assimilation of phosphorus (P), potassium (K), zinc (Zn), copper (Cu) and increases the resistance to plants under conditions of stress due to drought, salinity, frost, excessive rainfall and provides greater tolerance to diseases caused by nematodes and phytopathogenic fungi such as Phytophthora sp., Rizhoctonia sp., Pythium sp., Fusarium sp. among others.
- the present invention is formulated with elements that allow it to extend its shelf life, effectiveness and easy application alone or in a mixture: oligosaccharides (maltodextrin, maltose, dextrin, dextrose), polysaccharides (starch, glycogen, cellulose, chitin, paramilon, agarose, peptidoglycans, proteoglycans, hyaluronic acid, amylose, fructosan, kerate sulfate, dermatan sulfate, xylan, amylopectin), silicon dioxide, or hydrated aluminum silicate.
- oligosaccharides maltodextrin, maltose, dextrin, dextrose
- polysaccharides starch, glycogen, cellulose, chitin, paramilon, agarose, peptidoglycans, proteoglycans, hyaluronic acid, amylose, fructosan, kerate
- the present invention comprises the formulation of a plant biofortifier as a wettable powder to improve the efficiency in the assimilation of phosphorus (P), potassium (K), zinc (Zn), copper (Cu) and increase the resistance to plants under conditions of stress due to drought, salinity, frost, excessive rainfall and provide a greater tolerance to diseases caused by root pathogens such as nematodes and phytopathogenic fungi.
- the root volume increases to the absorption of water and nutrients, as well as providing stimulating hormones in plant growth thanks to the arbuscular vesicle mycorrhizae.
- the inventive protection of the formulation of a plant biofortifier is expected, which comprises a wettable powder specifically designed to improve efficiency in the different fertilization systems (DRENCH, Pivots, drip or band spray) the efficiency in the assimilation of phosphorus (P), potassium (K), zinc (Zn), copper (Cu) and increase resistance to plants under conditions of stress due to drought, salinity, frost, excessive rainfall and provide greater tolerance to diseases caused by pathogens Roots such as nematodes and phytopathogenic fungi (Phytophthora sp., Rizhoctonia sp., Pythium sp., Fusarium sp. among others).
- DRENCH different fertilization systems
- Pivots, drip or band spray the efficiency in the assimilation of phosphorus (P), potassium (K), zinc (Zn), copper (Cu) and increase resistance to plants under conditions of stress due to drought, salinity, frost, excessive rainfall and provide greater tolerance to diseases caused by pathogens Roots such as
- the mycorrhiza (from the Greek myces, fungus and rhiza, root) is a representation of the association of mycorrhizal fungi and the roots of plants.
- the term mycorrhiza was first described in 1877 by forest pathologist Frank when studying the roots of some forest trees. When the mycorrhizae come into contact with the root exudates of the plants, they respond and by penetrating the roots into the plant cells, forming a beneficial association for the plant and the fungus by supplying it with nutrients that the plant cannot absorb.
- Mycorrhizae are fundamentally important in some resource-limited ecosystems, without them growth and therefore yield may be reduced.
- Spanish patent ES 2397298T3 refers to liquid mycorrhiza compositions and methods for colonizing a plant, grass, branch or shrub with one or more mycorrhizae. Specifically, it relates to compositions that enhance the ability of mycorrhizae to colonize plant roots, resulting in superior efficacy of plant treatment formulations containing the mycorrhizae. Spanish patent ES2159258B1 is mentioned as the procedure for preparing a biofertilizer based on arbuscular mycorrhizal symbiosis-forming fungi.
- the Mexican patent MX / 2014/012524 (W02013 / 158900A1) comprises a combination of a phytate and a variety of microorganisms that include the fungus Tr ⁇ choderma virens, Bacillus amyloliquefaciens and also one or a mixture of mycorrhizal fungi that are placed in the rhizosphere of the plant allowing them to colonize said root vegetable; in addition, a method to increase the yield of the plant comprising: placing a combination of a phytate and a plurality of microorganisms comprising a Tr ⁇ choderma virens fungus, a Bacillus amyloliquefaciens bacterium and one or a mixture of mycorrhizal fungi in the rhizosphere of the plant a way that allows microorganisms to colonize said plant root.
- the Spanish patent ES2190286T3 comprises a fertilizer for taller plants, existing in granulated form, which contains at least 50 percent by weight of malt germs, which are produced in the malting of cereals for the preparation of beer and separated of the malt grain, where the fertilizer is preferably and essentially constituted by this type of malt germs, characterized in that each ton of grains contains at least 10g of mycorrhizal spores, preferably at least 25g of mycorrhizal spores and / or at least 5 weight percent mycelia, preferably at least 15 weight percent mycelia of at least one species of mycorrhizal fungus.
- Document C04600641 A1 describes a liquid biological fertilizer made up of fungi of plant origin, non-pathogenic for man of the mycorrhizal genus, capable of absorbing nutrients that are not very mobile from the soil such as phosphorus, sulfur, potassium, zinc, resulting in growth from the roots of plants and by Consequently, improving the productivity and vigor characteristics of all types of plants.
- Document AR100735 refers to a method to improve the growth, development and productivity of non-legume plants by implementing a composition comprising at least one mycorrhiza and at least one yeast extract, and optionally a substrate;
- the present application also refers to a composition of this type, and, when it comprises a substrate, to a process for its preparation.
- Document ES2201661 refers to methods and compositions to improve the quality of the grass of a lawn by using VA mycorrhiza as a growth retarder of Poa annua. More particularly, the invention relates to the control, reduction or elimination of undesirable grasses in a lawn, especially in a high quality lawn consisting mainly of grass grasses, such as Agrostis stolonifera or Festuca species.
- Document ES2659385 describes the use of a fertilizer that affects the distribution of plant biomass. More specifically, the fertilizer can stimulate root growth, fine root development, increase the number of root tips, and mycorrhiza development. Furthermore, the invention provides a method of using the fertilizer for modulation of the root fraction of the biomass.
- Figure 1b shows the beneficial effect on wheat root elongation and root density through the use of plant biofortifier 15 days after germination. On the right, the treatments that contain the plant biofortifier are observed and on the left side, the control treatment.
- Figure 1c shows the beneficial effect on wheat root elongation and root density through the use of plant biofortifier 30 days after germination. On the left, the treatments containing the plant biofortifier are observed and on the right side, the control treatment.
- Figure 1d shows the spores of the vesicle arbuscular mycorrhiza belonging to the plant biofortifier, the spores were isolated from the mixture for their observation.
- Figure 1e shows the density of the root biomass mentioned in example 3.
- mycorrhiza-plant there is some uncertainty of new technologies where the selected mycorrhiza is not adapted to the crops with greater economic activity, pests and different environmental conditions, likewise a mixture of different plant compounds that fortify and enhance the symbiont activity has not been considered. mycorrhiza-plant.
- the need for good fertilization conditions are the most important and critical factors for optimal performance.
- the objective of fertilization is to carry elements necessary to nourish the plant, however, there are problems with traditional granular fertilizers added in irrigation, drench and pivoting systems in which there is precipitation if the solubility of the fertilizer or the product is exceeded. , the precipitate is deposited on the walls of the tubes, in the holes of the drippers and in the sprinklers, completely plugging the system.
- the present invention is located in the area of Agricultural Biotechnology, it refers to a biological fortifier such as wettable powder from vesicle arbuscular mycorrhiza (VAM) and plant nutrients to improve crop yield.
- VAM vesicle arbuscular mycorrhiza
- the formulation of the biological fortifier is designed with a consortium of spores belonging to the strains of mycorrhizal fungi vesicle arbuscular (VAM): Glomus geosporum, Gigaespora margarita, Glomus fasciculatum, Glomus constrictum, Glomus tortuosum and Glomus intraradices, in addition the formulation is composed of extracts of humic and fulvic acids, extract of yucca (Yucca schidigera), extract of seaweed (Ascophyllum nodosum) and natural rooting agents (indoleacetic acid).
- VAM mycorrhizal fungi vesicle arbuscular
- the product improves the efficiency in the assimilation of phosphorus (P), potassium (K), zinc (Zn), copper (Cu) and increases the resistance to plants under conditions of stress due to drought, salinity, frost, excessive rainfall, It also has a particle size that avoids sedimentation, segregation and deposit in conventional fertilization systems and provides greater tolerance to diseases caused by Phytophthora sp., Rizhoctonia sp., Pythium sp., Fusarium sp. among others.
- the present invention is located in the area of Agricultural Biotechnology, it refers to a plant fortifier as a wettable powder with a particle size that allows optimal assimilation of crops, protection from the roots and blocking by sedimentation of nozzles and conventional systems of fertilization.
- An effective composition of a biological fortifier is described as a wettable powder to increase yields thanks to its formulation.
- the present invention is described from the following 4 perspectives: 1) Biological composition as microbial active ingredient, 2) Active ingredients as nutrients and plant growth enhancers, 3) Inert in the formulation and 4) Final particle size.
- the formulation of the biological fortifier is designed with a consortium of spores alone or in a mixture belonging to the strains of mycorrhizal fungi vesicle arbuscular (VAM): Glomus geosporum, Gigaespora margarita, Glomus fasciculatum, Glomus constrictum, Glomus tortuosum and Glomus intraradices which is sumistradaes. in the formulation in a percentage of 0.1-50% (w / w) (containing a concentration of 25-110 propagules / g) as a biological active ingredient. 2) Active ingredients as nutrients and plant growth enhancers.
- VAM mycorrhizal fungi vesicle arbuscular
- a formulation for the biological composition comprising of extracts of humic and fulvic acids in a concentration of 0.1% to 25%, extract of yucca (Yucca schidigera) in a concentration of 0.01% to 10%, extract of seaweed (Ascophyllum nodosum) in a concentration of 0.01 to 10% and natural rooters (2,4-D-indoleacetic acid) that ensure a concentration of 0.001% to! 1%.
- oligosaccharides maltodextrin, maltose, dextrin, dextrose
- poiisaccharides starch, glycogen, cellulose, chitin, paramilon, agarose, peptidoglycans, proteoglycans, hyaluronic acid, amylose, fructosan, keratin sulfate, dermatan sulfate, xylan, amylopectin
- the final formulation should contain a particle size between 177 microns and 105 microns at a pH of 8-10 for optimal performance.
- the mixture of the elements that make up the vegetable fortifier motive of the present invention can be mixed according to the following order: a) Weigh each of the nutrients that the biological composition comprises. b) Weigh and add the mycorrhizal spore consortium according to the mesh and add them to the mixer. c) Add the humic and fulvic acids in the mixer. d) Add the extract of seaweed and yucca in the mixer. e) Mix until the composition is homogeneous. f) Weigh and add the inert compounds in the mixer.
- the following example demonstrates the biological activity of the biofortifier from vesicle arbuscular mycorrhiza (VAM) and plant nutrients to improve crop yield. More specifically, the action of the biological fortifier as an inoculant in the tomato crop (Solanum lycopersicum) belonging to the Solanaceae family is demonstrated.
- VAM vesicle arbuscular mycorrhiza
- Phenological state of the plant In vegetative development, flowering and harvest of the tomato crop.
- Soil type clay.
- Experimental design Random blocks with four repetitions. The experimental unit with 2 furrows 1.0m wide by 60m long, which gives an experimental area of 1,200m 2 . An analysis of variance and a mean separation test were performed with the Tukey test at 95% reliability. Three doses of the biological fortifier in question were evaluated, a regional control and an absolute control (Table 1).
- Table 1 Treatments and doses evaluated to know the biological effectiveness of the freeze-fortifying agent in tomato (Solanum lycopersicum). Each treatment was applied 2 times to the soil in drip irrigation, in doses of 1.0, 1.5 and 2.0 kg for the application in transplantation and flowering, for the surface to be treated according to the experimental design. Two applications were made to the soil in drip irrigation, in transplantation and flowering, on the experimental units for each treatment. Variables for estimating biological effectiveness: a) Periodic growth: the height of the plants was estimated at 15, 30, 45 and 60 days after the first application. The measurement was carried out on 10 plants taken at random in each experimental unit (complete rows). b) Stem thickness.
- Stem thickness at ground level was determined 45 days after transplantation in 10 random plants per experimental unit, in 10 plants per experimental unit. c) Distance from the head to the flowering bouquet. It was evaluated 45 days after transplantation, in 10 plants per experimental unit. d) Distance between the full fruit bunch and the flowering bunch. It was evaluated 60 days after transplantation, in 10 plants per experimental unit. e) Length of sheets. The length of leaves in complete development, in the middle part of the plant at 45 days after transplantation, was evaluated in 10 leaves per experimental unit. f) Radical length. It was evaluated 90 days after transplantation, in 5 plants per experimental unit. g) Number of compound leaves per plant. The number of leaves per plant was counted in 5 plants per experimental unit 45 days after transplantation.
- Table 4 Treatments and doses evaluated to know the biological effectiveness of the fortifier at 30 days in the tomato crop (Solanum lycopersicum). Third evaluation: 45 days. In the analysis of variance table for plant growth in cm at 45 days, 4 statistical groups can be observed (Table 5). Treatment T3 (2.0 kg / ha) presents the highest average for height with 98.80 cm, while the absolute control T5 (0 kg / ha) presents an average of 87.07 cm.
- Table 9 Evaluation of the distance between the complete fruit bunch and the flowering bunch in the study of the effect of the biofortificante in the tomato crop (Solanum lycopersicum). E. Length of leaves.
- G Number of compound leaves per plant. The formation of 4 statistical groups (A, AB, B and C) was observed; Treatment T3 (2.0 kg / ha) presented the highest average of 58.65 for the number of leaves and the absolute control obtained an average of 39.40 leaves (Table 12).
- group A formed by the high doses T3 (2.0 kg / ha) with the highest average of 203.70 ton / ha
- group AB formed by the average treatment T2 (1.5Kg / ha)
- group B formed by the low dose T1 (1.0 Kg / ha)
- the regional control 1.0 kg / ha
- group C formed by the absolute control (0 kg / ha) with an average of 139 ton / ha (Table 13).
- the doses of 1.0, 1.5 and 2.0 kg / ha of the biofortifier were effective to increase the yield of the tomato crop (Solanum lycopersicum) because when using it it is possible to obtain a greater number of fruits per plant and a higher quality of these fruits and consequently an increase of 30%.
- Phosphorus There is a significant difference between the treatments with application and the absolute control, the highest mean obtained was from the T3 treatment (2.0g / ha) with 0.51%. Potassium. There is a significant difference between the treatments with application and the absolute control, the highest mean obtained was from the T3 treatment (2.0 kg / ha) with 3.35%. Calcium (Ca): There is a significant difference between the treatments with application and the absolute control, the highest mean obtained was from the T3 treatment (2.00 kg / ha) with 0.2%.
- Zinc (Zn) There is a significant difference between the treatments with application and the absolute control, the highest mean obtained was from the T3 treatment (2.0 kg / ha) with 0.39 ppm.
- Phosphorus There is a significant difference between the treatments with application and the absolute control, the highest mean obtained was from the T3 treatment (2.0 kg / ha) with 0.91%. Potassium. There is a significant difference between the treatments with application and the absolute control, the highest mean obtained was from the T3 treatment (2.0 kg / ha) with 5.62%. Calcium (Ca): There is a significant difference between the treatments with application and the absolute control, the highest mean obtained was from the T3 treatment (2.0 kg / ha) with 4.35%.
- Table 17 Evaluation of the nutritional analysis in the plant, in the study of evaluation of the effect of the biofortificante in the tomato crop (Solanum lycopersicum).
- the experimental unit was made up of 3 beds of 1.3 m wide equal to 3.9 m, by 3.0 m long, equivalent to 11.7 m 2 , giving a total of 46.8 m 2 per treatment. A total area of 280.8 m 2 was used .
- Application forms Drench.
- Application equipment Manual backpack sprayer.
- Biological effectiveness measurement parameter Two applications were made in the indicated stage, considering the following variables: 1. Phytotoxicity. It was evaluated 7 days after each application, using the percentage scale proposed by the European Weed Research Society (Table 2).
- Stem diameter It was measured with a vernier in 3 random plants in the center of the experimental unit (repetition), at 0 days before the first application and 7 days after the first and 14 days after the second application. . The results were expressed in mm.
- Number of leaves The number of leaves of 3 plants randomly sampled in the center of the experimental unit (repetition), 14 days after the last application, was counted. The results were expressed as a numerical value.
- Fresh root weight (g) It was determined in two plants randomly sampled per experimental unit (repetition), the roots were extracted, washed and weighed with the help of a digital scale with a capacity of 500 g., At 14 days after the last application, the results were expressed in g.
- Root dry weight It was determined in two plants randomly sampled per experimental unit (repetition) at 14 days after the last application, the roots were dried in an oven in the laboratory, they were weighed with the help of a digital scale with a capacity of 500 g. The results were expressed in g.
- Chlorophyll content in leaves Two leaves were taken in three plants per repetition, which was measured with the SPAD method, which determines the relative amount of chlorophyll present through the measurement of the absorption of the leaves in two wavelength regions; in the red and near infrared regions. Using these two transmissions, the meter calculates the SPAD numerical value that is proportional to the amount of chlorophyll present in the leaf and consequently of nitrogen, 14 days after the second application.
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Pest Control & Pesticides (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US17/642,847 US20220388925A1 (en) | 2019-09-12 | 2020-07-03 | Plant strengthener based on vesicular-arbuscular mycorrhizae, extracts and plant nutrients |
PE2022000413A PE20220602A1 (es) | 2019-09-12 | 2020-07-03 | Fortificante vegetal a base de micorrizas vesiculo arbusculares, extractos y nutrientes vegetales |
ES202290024A ES2908131B2 (es) | 2019-09-12 | 2020-07-03 | Fortificante vegetal a base de micorrizas vesiculo arbusculares, extractos y nutrientes vegetales |
BR112022004686A BR112022004686A2 (pt) | 2019-09-12 | 2020-07-03 | Fortificante vegetal à base de micorrizas vesicular-arbusculares, extratos e nutrientes vegetais |
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Application Number | Priority Date | Filing Date | Title |
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MX2019012107A MX2019012107A (es) | 2019-09-12 | 2019-09-12 | Fortificante vegetal a base de micorrizas vesículo arbusculares, extractos y nutrientes vegetales. |
MXMX/A/2019/012107 | 2019-09-12 |
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WO2021049927A1 true WO2021049927A1 (es) | 2021-03-18 |
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PCT/MX2020/050014 WO2021049927A1 (es) | 2019-09-12 | 2020-07-03 | Fortificante vegetal a base de micorrizas vesículo arbusculares, extractos y nutrientes vegetales |
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US (1) | US20220388925A1 (es) |
BR (1) | BR112022004686A2 (es) |
ES (1) | ES2908131B2 (es) |
MX (1) | MX2019012107A (es) |
PE (1) | PE20220602A1 (es) |
WO (1) | WO2021049927A1 (es) |
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CN117158282B (zh) * | 2023-10-26 | 2023-12-26 | 内蒙古工业大学 | 一种用于沙漠化防治的生物质复合材料及其制备方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05153864A (ja) * | 1991-12-09 | 1993-06-22 | Idemitsu Kosan Co Ltd | 水稲の栽培方法 |
WO2011152700A1 (en) * | 2010-06-04 | 2011-12-08 | Biotrack Technology (M) Sdn Bhd | A palm growth underground formulation |
WO2012121579A1 (es) * | 2011-03-10 | 2012-09-13 | Munoz Santiago Antonio | Inoculante endomicorricico formador de micorrizas arbusculares aplicado a plántulas de hortalizas y frutales |
-
2019
- 2019-09-12 MX MX2019012107A patent/MX2019012107A/es unknown
-
2020
- 2020-07-03 PE PE2022000413A patent/PE20220602A1/es unknown
- 2020-07-03 ES ES202290024A patent/ES2908131B2/es active Active
- 2020-07-03 US US17/642,847 patent/US20220388925A1/en active Pending
- 2020-07-03 WO PCT/MX2020/050014 patent/WO2021049927A1/es active Application Filing
- 2020-07-03 BR BR112022004686A patent/BR112022004686A2/pt unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05153864A (ja) * | 1991-12-09 | 1993-06-22 | Idemitsu Kosan Co Ltd | 水稲の栽培方法 |
WO2011152700A1 (en) * | 2010-06-04 | 2011-12-08 | Biotrack Technology (M) Sdn Bhd | A palm growth underground formulation |
WO2012121579A1 (es) * | 2011-03-10 | 2012-09-13 | Munoz Santiago Antonio | Inoculante endomicorricico formador de micorrizas arbusculares aplicado a plántulas de hortalizas y frutales |
Non-Patent Citations (1)
Title |
---|
EDIFARM: "Vademecum agricola 2016", 2016, EDIFARM Y COMPAÑÍA, ISBN: 978-9942-906-33-5, pages: 144 * |
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Publication number | Publication date |
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PE20220602A1 (es) | 2022-04-25 |
ES2908131B2 (es) | 2023-06-19 |
MX2019012107A (es) | 2021-03-15 |
ES2908131A1 (es) | 2022-04-27 |
BR112022004686A2 (pt) | 2022-08-30 |
US20220388925A1 (en) | 2022-12-08 |
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