WO2021039758A1

WO2021039758A1 - Poison bait for bees

Info

Publication number: WO2021039758A1
Application number: PCT/JP2020/031949
Authority: WO
Inventors: 練阿部; 和輝前田
Original assignee: アース製薬株式会社
Priority date: 2019-08-27
Filing date: 2020-08-25
Publication date: 2021-03-04
Also published as: JPWO2021039758A1; JP6888178B1; JP2021121610A; JP2021107409A

Abstract

The present invention addresses the problem of providing a highly palatable poison bait for bees. A solution is a poison bait for bees containing the following components (A) and (B). The component (A) is fipronil and the component (B) is at least one selected from diethylene glycol monoethyl ether, ethyl lactate, polyoxyethylene hydrogenated castor oil, polyglyceryl monolaurate, polyoxyethylene sorbitan fatty acid esters, and polyethylene glycol monolaurate. Further, the poison bait for bees contains the following component (C). The component (C) is non-centrifugal sugar and/or honey.

Description

Bee poison bait

The present invention relates to a poison bait for bees. More specifically, the present invention relates to an invention characterized by containing fipronil and a specific solvent and / or a surfactant, and also containing fipronil and honey-containing sugar and / or honey.

In recent years, damage caused by bees has increased and has become a problem. About 3000 species of bees are known in Japan, and it is said that about 20 species of carnivorous bees have strong biting properties. Among carnivorous bees, bees belonging to the family Vespids, such as wasps and paper wasps, are highly aggressive species that cause anaphylactic shock when bitten and can be attacked multiple times due to the structure of the poison stinger. , It is one of the pest species with very high demand for extermination, and quick-acting is required for its extermination.
Carnivorous bees may nest under the eaves or attic of private houses, and in urban areas, the area of human residence and the range of activities of carnivorous bees overlap, so damage from bites tends to occur frequently. .. Since carnivorous bees are warlike, they can be attacked and damaged if they approach the nest without their knowledge. For example, the number of people who die from being bitten by a carnivorous bee is reported to be about 10 to 20 every year.

As a current method for exterminating bees, a liquid agent or an aerosol agent containing an insecticidal active ingredient is generally sprayed directly onto the bees to exterminate them, and a pyrethroid insecticide having a rapid effect is used. Many aerosol agents contained as an active ingredient have been proposed (for example, Patent Documents 1 to 3 and the like). However, even if these aerosols are used, if it is not possible to spray a sufficient amount on each individual, or in the time until the insecticidal effect appears, the excited bee releases the warning pheromone, and more. It attracted many excited bees and was sometimes attacked by these bees.
Under these circumstances, as a method for exterminating bees other than liquids or aerosols, there are expectations for bee poison bait agents that can exterminate bees more safely simply by installing them.

JP-A-2017-178793 JP-A-2015-093846 Japanese Unexamined Patent Publication No. 2011-144151

In order to enhance the bee extermination effect of the poison bait, it is effective to maintain the same eating habits as those without them even if a solvent or a surfactant is added.
Therefore, an object of the present invention is to provide a highly edible poison bait for bees.

As a result of intensive research to solve the above problems, the present inventor selected fipronil as an insecticidal component, which can obtain high activity in a small amount. In order to obtain a stable insecticidal effect of fipronil, by using a specific solvent and / or a surfactant in combination with fipronil, fipronil can be uniformly dispersed in the preparation, and even if a solvent or a surfactant is added. , It has been found that the bee eating habits equivalent to those without them can be maintained, and the above-mentioned problems have been solved.

Specifically, the gist of the present invention is as follows.
1. 1. A poison bait for bees containing the following component (A) and the following component (B).
Component (A): Fipronyl component (B): One or more selected from diethylene glycol monoethyl ether, ethyl lactate, polyoxyethylene hydrogenated castor oil, polyglyceryl monolaurate, polyoxyethylene sorbitan fatty acid ester, and polyethylene glycol monolaurate. Further, 1. The following component (C) is contained. Bee poison bait described in.
Ingredient (C): sugar-containing sugar and / or honey 3. A poison bait for bees containing fipronil and honey-containing sugar and / or honey.

Since the bee poison bait of the present invention contains fipronil having a high insecticidal effect as the component (A), a high bee extermination effect can be obtained. Moreover, by blending the component (B), the solubility of fipronil can be enhanced, and the bee eating habits equivalent to those without the component (B) can be maintained, so that a high extermination effect on bees can be exhibited. Can be useful.
Further, in the bee poison bait of the present invention, by using fipronil in combination with honey-containing sugar and / or honey, it is possible to suppress the decrease of fipronil due to decomposition or the like, so that the bee poison bait of the present invention can be stored for a long period of time. In addition to improving stability, it can maintain a high extermination effect on bees for a long period of time.

It is an exploded perspective view of the structure which excluded the liquid-absorbing member from the specific aspect of the bee poison bait container which stores the bee poison bait of this invention. It is a perspective view which shows the state which the inner lid and the umbrella of the bee poison bait container shown in FIG. 1 are removed.

Hereinafter, the poison bait for bees of the present invention will be described in detail.
The bee poison bait of the present invention contains fipronil as a component (A).
<Ingredient (A)>
Fipronil is a generic name and its chemical name is 5-amino-1- [2,6-dichloro-4- (trifluoromethyl) phenyl] -4- (trifluoromethylsulfonyl) -1H-pyrazole-3-. Carbonitrile is a compound having the following chemical structure.

Fipronil is a compound that exhibits insecticidal activity by inhibiting neurotransmission by γ-aminobutyric acid, which is a neurotransmitter. Since the onset of the effect is delayed, it is also a compound used as an insecticidal component of a cockroach poison bait by utilizing the time difference between eating and returning to the home until death.
The component (A) of the present invention is preferably contained in the bee poison bait in the range of 0.0001% by weight or more and 10% by weight or less, and preferably in the range of 0.001% by weight or more and 5% by weight or less. Is more preferable.

<Ingredient (B)>
The bee poison bait of the present invention is selected from diethylene glycol monoethyl ether, ethyl lactate, polyoxyethylene hydrogenated castor oil, polyglyceryl monolaurate, polyoxyethylene sorbitan fatty acid ester, and polyethylene glycol monolaurate as the component (B). It contains more than a seed.
The component (A) fipronil is a substance that can obtain high activity in a small amount, and it is preferable to disperse it uniformly in the preparation in order to obtain a stable effect. However, the water solubility of fipronil is 3.78 × 10 ³ μg / L (20 ° C, pH 6.6), which is a compound that is extremely difficult to dissolve in water. Therefore, in order to obtain a stable poison bait for bees, a solvent is used. And surfactants are needed.
Therefore, as a result of various studies on solvents and surfactants, among the solvents and surfactants used, there are many solvents and surfactants that inhibit bee eating habits, but no solvent or surfactant is blended. It was clarified that there are solvents and surfactants that can maintain the same bee eating habits. The solvent and surfactant selected in the study are the components (B) of the present invention.
The component (B) of the present invention is preferably contained in the poison bait for bees in the range of 0.005% by weight or more and 50% by weight or less, and more particularly in the range of 0.05% by weight or more and 30% by weight or less. The range of 0.05% by weight or more and 10% by weight or less is more preferable, and the range of 0.1% by weight or more and 5% by weight or less is particularly preferable.

<Component (C)>
The honey poison bait of the present invention preferably contains honey-containing sugar and / or honey as the component (C). Molasses-containing sugar is one of the types produced by a method for producing sugar, and is obtained by crystallizing molasses containing abundant minerals and the like without separating it from crystals. Specifically, for example, brown sugar (brown sugar), processed brown sugar, white sugar, red sugar, Wasanbon sugar, sorghum sugar, maple sugar and the like are included.
The component (C) of the present invention contains, for example, sotolon, furfuryl alcohol, furfural, etc. in brown sugar (brown sugar), sotolon, furfuryl alcohol, etc. in maple sugar, sotolon in honey, etc. Each is characterized by containing isovaleraldehyde, methional and the like.
The fipronil of the component (A) is a compound known to be a decomposition product of fipronil sulfone or the like by decomposition or oxidative action, but by using the component (C) of the present invention in combination, the reduction of fipronil due to this decomposition or the like is performed. It became clear for the first time that this was suppressed.
That is, by using fipronil in combination with honey-containing sugar and / or honey, a decrease due to decomposition of fipronil or the like can be suppressed. Therefore, for bees of the present invention containing fipronil, honey-containing sugar and / or honey. The poison bait improves long-term storage stability and can maintain a high extermination effect on bees for a long period of time.
In order to use the fipronil of the component (A) and the honey-containing sugar and / or honey of the component (C) in combination and suppress the decrease due to the decomposition of fipronil, the honey-containing sugar and / or honey of the component (C) is used. , It is preferable that the content is 1% by weight or more based on the total amount of the poison bait for bees.

The bee poison bait of the present invention contains honey-containing sugar and / or honey as the component (C), and when the sugar content (Brix) is in the range of 40 or more and less than 83, the edible property of the bee and the nest of the bee It is preferable because it can promote the transport of the poisonous bait for bees of the present invention to the bees, exerts a high extermination effect on bees, and can efficiently disintegrate the entire beehive, which is useful.
Further, when the honey poison bait agent of the present invention contains honey-containing sugar and / or honey as the component (C) and the sugar content (Brix) is 40 or more, the poison bait on a carrier having a large contact with air. Even when the agent is impregnated, adhered or applied, the generation of mold and the like is greatly reduced, and the putrefaction of the poison bait is suppressed, so that the effect of exterminating the bees is not reduced.
Further, by setting the sugar content (Brix) of the bee venom bait of the present invention in the range of 40 or more and less than 83, the distance from the beehive is more than 1 meter and the sugar content is within 3 meters from the bee's movement line. This is preferable because the bees recognize the poison bait as a new bait or a new feeding ground at an early stage, and as a result, the entire beehive can be disintegrated at an early stage.

When the sugar content (Brix) of the poison bait for bees of the present invention is in the range of 40 or more and less than 83, the sugar content (Brix) is the reading of the sugar refractometer at 20 ° C., and is used for bee extermination. It means a value measured at 20 ° C. using a digital refractometer PAL-1 (manufactured by Atago Co., Ltd.) for the entire poison bait. Since the measurement range of the digital refractometer PAL-1 (manufactured by Atago) is 0.0 to 53.0 (Brix), if the sugar content (Brix) is higher than 53.0, use ion-exchanged water 10 times. It is diluted and measured, and the value is converted to a value 10 times. In the case of a non-liquid preparation, the sugar content is measured by diluting it 10 times with ion-exchanged water and multiplying the measured value by 10 times.
The sugar content (Brix) of the bee poison bait of the present invention is preferably 40 or more, more preferably 50 or more, and even more preferably 60 or more. The upper limit of the sugar content (Brix) is preferably less than 83.
By setting the sugar content (Brix) of the bee poison bait of the present invention in the range of 40 or more and less than 83, not only the eating habitability of the bee is improved, but also the behavior of the bee to carry the poison bait to the nest is promoted. Can be done. As a result, the transported poison bait is given to other bees in the nest, and the poison bait spreads throughout the nest, exhibiting an excellent extermination effect in which the nest collapses. That is, by attracting the bees from the nest to the poison bait and bringing the poison bait back into the nest, the entire nest can be finally destroyed, which is useful.

By setting the sugar content (Brix) of the bee poison bait of the present invention to a range of 40 or more and less than 83, the poison bait is arranged within a range of more than 1 meter from the beehive and within 3 meters from the bee's movement line. It is preferable because the bees recognize the agent as a new bait or a new feeding ground at an early stage, and as a result, the entire beehive can be disintegrated at an early stage. This suitable arrangement is that if the poison bait is placed within 1 meter from the beehive, the bees will be alert and difficult to approach the newly emerging artificial poison bait, and more than 3 meters from the bee's flow line. If the poison bait is placed in a remote place, it is considered that it takes a very long time for the bees to recognize the poison bait as a new bait or a new feeding ground.
That is, by arranging the sugar content (Brix) of the bee poison bait of the present invention in the range of 40 or more and less than 83, away from the beehive and within 3 meters from the bee's movement line. It is speculated that the bees reach the poisonous bait early and the transport of the poisonous bait to the nest is promoted, and as a result, the entire beehive collapses early.
The "traffic line of a bee" in the present invention means a trajectory in which a bee moves back and forth between the nest and a conventional feeding ground starting from the beehive.

<Bee>
The bees in which the poison bait for bees of the present invention exerts an excellent extermination effect are mainly targeted at bees belonging to the family Vespidae, but membranes of bees such as Vespids, bear bees, bees, ammophilinae, and potter wasps. Examples include those belonging to the Hymenoptera pest. Among them, as the wasp family bee, a bee belonging to the wasp subfamily (Vespinae) and the paper wasp subfamily (Polistinae) can be mentioned.
Examples of bees belonging to the subfamily Wasp include giant hornets, wasps, wasps, hornets, hornets, wasps, wasps, wasps, fern wasps, wasps, wasps, and wasps.
In addition, as bees belonging to the paper wasp subfamily, for example, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps. Can be mentioned.

<Attractive ingredient>
By using the poison bait for bees of the present invention in combination with a known attracting component, bees can be attracted and surely exterminated. Known attracting ingredients include, for example, balsamic vinegar, apple vinegar, rice vinegar, brown rice vinegar, lees vinegar, soybean vinegar, black vinegar, wine vinegar, sudachi vinegar, red vinegar, persimmon vinegar, malt vinegar, purple potato vinegar, and sugar cane. Vinegar such as vinegar, lactic acid products (lactic acid bacteria beverages, etc.), sugars, starch sugar, honey, sugar honey, waste sugar honey, fruits, processed fruit products, fruit juice, fruit juice beverages, fruit liquor, beer, Japanese sake, shochu, whiskey, brandy, Based on liquor such as vodka, lamb, gin, tekira, shokoshu, white liquor, old liquor, sake lees, seafood, processed seafood, seafood extract, meat, processed meat, meat extract, fragrance, etc. There may be. Among them, it is preferable to adopt the honey-containing sugar and / or honey of the component (C) as an attracting component in consideration of suppressing the decrease due to the decomposition of fipronil of the component (A), and the honey-containing sugar is particularly preferable. Among the above-mentioned attracting components, liquid ones are particularly preferable. Further, if a moisturizing component such as an aliphatic polyhydric alcohol such as glycerin, a sugar alcohol such as sorbitol, or a thickening polysaccharide such as xanthan gum is contained, the effect of the attracting component can be exhibited for a long period of time.

<Insecticidal ingredients other than ingredient (A)>
In addition to the component (A) fipronil, a known insecticide can be used in combination with the bee poison bait of the present invention. Known pesticides that can be used in combination include, for example, natural pyrethrins, alesulins, resmethrins, flamethrins, prarethrins, terraresulins, phthalthrins, phenothrins, permethrins, ciphenothrins, cypermesrins, transfluthrins, metoflutrins, profluthrins, imiprothrins, empentrins, epentrins. , Pyrethroid compounds such as silafluofen, meperfluthrin, dimefluthrin; carbamate compounds such as propoxul, carbalyl; organic phosphorus compounds such as fenitrothione, diazinone, tetrachlorobinphos, DDVP; oxadiazole compounds such as metoxadiazone; Symphonamide compounds; Neonicotinoid compounds such as imidacloprid and dinotefuran; Insecticide immature hormone-like compounds such as pyriproxyfen, metoprene and hydroprene; Oxidative phosphorylation deconjugating agents such as sulfullamin; Anti-juvenile such as plecosene Hormone-like compounds; chitin synthesis inhibitors such as novallon, diflubenzurone, etoxazole; amidinohydrazone compounds such as hydramethylnone; various insecticides such as phytonchid, peppermint oil, orange oil, katsura oil, clove oil and other insecticides In addition, synergistic agents such as cinepyrin and piperonyl butoxide can also be used in combination. Insect growth regulators such as insect juvenile hormone-like compounds, anti-juvenile hormone-like compounds, and chitin synthesis inhibitors, which are less repellent, can also be preferably used in combination.

In addition, when used in combination with the component (A) fipronil, a highly water-soluble substance may be preferable in terms of formulation. For example, acephate, bamidione, methidathion (DMTP), phenobcarb (BPMC), ethiophencarb, cartap, thiocyclam, imidacloprid, thiacloprid, cyromazine, hostiazeto, acetamiprid, thiamethoxam, carbyl (NAC), clothianidin, pictrodine, etc. Among these, for example, dinotefuran (water solubility at 20 ° C.: about 54,000 ppm), thiamethoxam (water solubility at 20 ° C.: about 4100 ppm), imidacloprid (water solubility at 20 ° C.: about 510 ppm), phenocarb (BPMC, water at 20 ° C.). A water solubility of 500 ppm or more at 20 ° C. such as (solubility: about 610 ppm) is suitable as an insecticide to be combined with the poison bait for bees of the present invention.

<Formulation>
The bee poison bait of the present invention is formulated by containing component (A), component (B), and further component (C), or containing fipronil, sugar-containing sugar and / or honey. Further, a prepared product diluted with water is included. Among them, the formulated bee poison bait of the present invention is advantageous at the time of transportation and storage because it is compact and has excellent storage stability. Formulation types include liquid preparations such as oils, emulsions, wettable powders, flowables (suspensions in water, emulsions in water, etc.), gels, pastes, microcapsules, powders, granules, etc. Examples include solid preparations such as tablets. Above all, the liquid preparation is suitable because it is easy to dilute with water and there is little undissolved residue. As the water used for dilution, purified water, tap water, ion-exchanged water, distilled water, filtered water, sterilized water, groundwater, well water and the like are used.
The poison bait for bees of the present invention is impregnated, adhered or applied to a polymer such as a sponge, absorbent cotton, natural fiber, a non-woven fabric of synthetic fiber, a water-absorbent polymer, or a carrier such as a woven cloth, paper, or a porous body, and is applied to a container. It is preferable to store and use it. When the bee poison bait of the present invention is installed outdoors, there is a space for the opening in order to prevent rainwater or the like from infiltrating and diluting the bee poison bait of the present invention and reducing the bee extermination effect. However, it is preferable to provide a covering portion for preventing the ingress of rainwater and the like.

<Preparation aid>
In the formulation of the poison bait for bees of the present invention, a surfactant other than the component (B) can be used as long as the effect of the present invention is not impaired. As the surfactant, any of nonionic surfactant, anionic surfactant, cationic surfactant and amphoteric surfactant can be used without particular limitation, and among them, nonionic surfactant can be used. Activators or anionic surfactants are preferred.
Specifically, for example, as nonionic surfactants, sugar ester type, fatty acid ester type, vegetable oil type, alcohol type, alkylphenol type, polyoxyethylene-polyoxypropylene block polymer type, alkylamine type, bisphenol type. , Polyaromatic ring type. Examples of the anionic surfactant include carboxylic acid type, sulfonic acid type, sulfate ester type and phosphoric acid ester type. Examples of the cationic surfactant include ammonium type and benzalkonium type. Examples of amphoteric surfactants include betaine-type surfactants.
As the surfactant other than the component (B), either a single surfactant or a mixture of two or more thereof can be used.

The bee poison bait of the present invention can be formulated by containing components generally added to the preparation within a range that does not interfere with the effects of the present invention. Examples of components generally added to preparations include stabilizers, preservatives, colorants, accidental ingestion / accidental ingestion inhibitors, liquid carriers and the like. Examples of the stabilizer include antioxidants such as dibutylhydroxytoluene (BHT) and butylhydroxyanisole (BHA), ascorbic acid and the like. Examples of preservatives include sodium chloride, sorbic acid, sorbate, parahydroxybenzoic acid esters, thiabendazole and the like. Examples of the colorant include caramel color, cutinashi color, anthocyanin color, safflower color, flavonoid color, red No. 2, red No. 3, yellow No. 4, yellow No. 5, and the like. Examples of the accidental ingestion / accidental ingestion preventive agent include denatonium benzoate.

Examples of the liquid carrier used in the formulation include alcohols (methanol, ethanol, isopropyl alcohol, butanol, hexanol, ethylene glycol, etc.) and ethers (diethyl ether, ethylene glycol dimethyl ether, diethylene glycol monomethyl ether, propylene glycol monomethyl ether). , Tetrahydrofuran, dioxane, etc.), esters (ethyl acetate, butyl acetate, isopropyl myristate, etc.), ketones (acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone, etc.), aromatic or aliphatic hydrocarbons (xylene, toluene, etc.) Alkylnaphthalene, phenylxysilyl ethane, kerosine, light oil, hexane, cyclohexane, etc.), halogenated hydrocarbons (chlorobenzene, dichloromethane, dichloroethane, trichloroethane, etc.), nitriles (acetriform, isobutyronitrile, etc.), sulfoxides (dimethyl Sulfoxide, etc.), heterocyclic solvents (sulfolane, γ-butyrolactone, N-ethyl-2-pyrrolidone, N-octyl-2-pyrrolidone, 1,3-dimethyl-2-imidazolidinone), acid amides (N, N-dimethylformamide, N, N-dimethylacetamide, etc.), alkylidene carbonates (propylene carbonate, etc.), vegetable oils (soybean oil, cottonseed oil, etc.), vegetable essential oils (orange oil, hissop oil, lemon oil, etc.), and water. Be done.

<Poison bait container for bees>
The bee poison bait of the present invention is preferably stored in a container for use.
The shape and size of the container to be stored are not limited as long as the container can contain the poison bait for bees of the present invention, and may be in a form suitable for the place of use and the method of use. The material of this container is particularly limited as long as it is made of glass, metal, plastic, etc., or special paper having a waterproof or water-repellent function that prevents the poison bait for bees of the present invention from leaking from the container. Not done.
As an example of the aspect of the container, it is preferable to have a lid covering the opening of the container, and an opening through which a bee can enter is formed in either the lid or the container. It is preferable to form a plurality of openings in the container so that the bees can easily enter the container, and it is preferable to form two or more and five or less openings depending on the size of the container. .. Further, it is preferable that the bees that have entered the container have a shape that allows them to easily go out of the container after eating the poison bait for bees of the present invention.
The container may be provided with a transparent or translucent window corresponding portion so that the bees can be visually recognized, or may be a transparent or translucent container.
When the bee poison bait container is installed outdoors, a space is provided for the opening in order to prevent rainwater or the like from infiltrating and diluting the bee poison bait of the present invention and reducing the bee extermination effect. At the same time, it is preferable to provide a covering portion for preventing the ingress of rainwater and the like.
The bee poison bait container of the present invention is used by placing it on a flat place such as the ground or a veranda where it is not exposed to direct sunlight or the entrance of the container is not blocked, or from a flat surface of the flat place. It is preferable to hang it in a height range of 1 to 3 m. In particular, it is more preferable to install it within a range of more than 1 meter from the beehive and within 3 meters from the flow line of the bees. The "traffic line of the bee" means a trajectory in which the bee moves back and forth between the nest and the conventional feeding ground starting from the beehive.

<Specific example of bee poison bait container>
The configuration of the bee poison bait container 1 suitable for storing the poison bait of the present invention will be described with reference to FIGS. 1 and 2. The bee poison bait container 1 is configured so that the bee can easily invade and at the same time the human finger is less likely to touch the poison bait of the present invention, and the effective period of the bee poison bait can be extended.
The bee poison bait container 1 includes a lower container 10 (container body: bottom surface 101, side surface 102, protrusion 103, opening 105), an inner lid 20, an umbrella 30, and a liquid absorbing member 40.

The inner lid 20 is configured to be engaged with the outside of the lower container 10 and to be fixed to the lower container 10. A bee entrance 21 (opening) communicating with the lower container 10 is formed in the center of the inner lid 20. Further, the inner lid 20 is formed with a bee retention port 22 for the bees to anchor. The bee-retaining port 22 is inclined so that the inside is higher than the outer edge thereof (the height is increased from the outer edge of the bee-retaining port 22 toward the center side). Bees may be described as having a negative runnability, that is, the property of organisms traveling in the opposite direction of gravity. By providing the inclined surface 22A (distance D4 from the outer edge to the bee entrance / exit 21), it becomes possible to promote walking of the bee into the bee poison bait container 1. As shown in FIG. 1, a groove G extending in the radial direction may be provided between the adjacent inclined surfaces 22A. The groove G allows the bee to hook the leg, thus assisting the bee to climb the inclined surface 22A. The inclined surface 22A may be provided with a convex portion or an uneven portion.

By providing the inclined surface 22A, when a human finger is inserted through the gap between the umbrella 30 and the inner lid 20, the inserted finger comes into contact with the inclined surface 22A and is guided obliquely upward. Therefore, it is possible to guide the finger in the direction away from the liquid absorbing member 40 housed in the lower part of the lower container 10. Further, even if the joint of the finger is to be bent, the finger hits the umbrella 30 and becomes difficult to bend, so that it becomes difficult to touch the liquid absorbing member 40.
Further, by providing the inclined surface 22A, the wind passing through the bee poison bait container 1 is guided upward along the inclined surface 22A. Therefore, it is possible to suppress the wind from directly hitting the liquid absorbing member 40, suppress the fluctuation of the volatilization amount of the poison bait agent impregnated in the liquid absorbing member 40, and eventually the liquid absorbing member 40. It becomes possible to suppress fluctuations in the usable period.
Further, the bee retention port 22 is formed so as to project radially outward from the outer peripheral surface of the lower container 10. By providing the bee retention port 22 so as to project in this way, the bees can be easily anchored, and the bees can enter the inside of the lower container 10 through the bee entrance / exit 21 formed in the center of the inner lid 20.
A plurality of engaging hole portions 23 for engaging the lower end of the umbrella 30 (lower end of the side wall portion 32) are formed in the peripheral portion of the inner lid 20.

The umbrella 30 is engaged with the inner lid 20 so as to cover the upper part of the bee doorway 21. When the umbrella 30 is engaged with the inner lid 20, the liquid absorbing member 40 exposed to the outside through the bee entrance / exit 21 is covered from above by the umbrella 30, and water or the like (for example, rain) is applied to the liquid absorbing member 40 from the outside. ) Can be suppressed from hitting directly. Further, the inclined surface 22A of the inner lid 20 described above can also prevent water or the like from entering the lower container 10. The umbrella 30 is provided with an arch-shaped opening 31 that communicates the bee entrance 21 and the outside of the bee poison bait container 1. The bees anchored at the bee retention port 22 can enter the inside of the bee poison bait container 1 (inside the umbrella 30) through the arch-shaped opening 31, and further enter the inside of the lower container 10 through the bee entrance 21. The side wall portions 32 located on both sides of the arch-shaped opening 31 engage with the inner lid 20, and the umbrella 30 and the inner lid 20 are fixed. Here, the height of the arch-shaped opening 31 with respect to the inclined surface 22A of the inner lid 20 (the vertical distance between the inclined surface 22A and the intermediate portion of the arch-shaped opening 31) is 10 mm or more and 35 mm or less. It is preferably present, and more preferably 10 mm or more and 30 mm or less. When the height of the arch-shaped opening 31 from the inner lid 20 is 10 mm or more, even a large bee such as a wasp can invade the bee poison bait container 1. On the other hand, if the height of the inner lid 20 and the arched opening is larger than 35 mm, a human finger can easily come into contact with the poison bait agent of the present invention. In addition, large insects other than bees invade.
In addition, on the upper surface of the umbrella 30, a hooking portion 35 for hooking and arranging the bee poison bait container 1 on an external member is provided.

The liquid-absorbent member 40 is an impregnated body impregnated with the poison bait agent of the present invention for feeding bees.
As shown in FIG. 2, the liquid absorbing member 40 is formed with a hole 42 (recess) extending in the direction from the upper surface 40a side of the main body portion (liquid absorbing member main body) toward the lower surface. The hole 42 is not limited to the embodiment formed only in the liquid absorbing member 40, and may be formed between the liquid absorbing member 40 and the lower container 10, for example, or is formed only in the lower container 10. You may. When the hole 42 is formed only in the lower container 10, if the hole 42 is formed along the peripheral edge of the liquid absorbing member 40, the bee that has entered the inside of the hole 42 impregnates the liquid absorbing member 40. It is suitable because the poison bait agent of the present invention can be eaten.

The hole 42 has an opening shape for a bee to enter the inside thereof, in other words, an opening for the bee's head to enter inside. Specifically, the diameter of the hole 42 is preferably in the range of 5 to 30 mm. If the diameter of the hole 42 is larger than 30 mm, the inside of the hole 42 tends to dry, and if the diameter of the hole 42 is smaller than 5 mm, it becomes difficult for the bee head to enter the inside of the hole 42. Bees have a habit of poking their necks into their burrows (for example, wasps and paper wasps poke their heads into their burrows to get nutrients from the larvae in their burrows (thick liquid collects below the burrows 42). In this case, wasps and paper wasps have a habit of sticking their heads and licking the liquid below the hole 42), so bees use this habit to eat the poison bait of the present invention contained inside the hole 42. .. Therefore, even if the upper surface 40a side of the liquid-absorbent member 40 is dried, the poison bait agent of the present invention is held inside the hole 42 formed in the liquid-absorbent member 40 by utilizing the habit of such a bee. Because the bees eat, the shelf life of the poison bait can be extended.
The hole 42 is formed so as to extend from the upper surface 40a side to the lower surface side of the liquid absorbing member 40, and the extending direction thereof includes an arbitrary direction.
Further, the hole 42 does not have to penetrate the liquid absorbing member 40 as long as it has an opening sufficient for the bee's head to enter. For example, if the thickness of the liquid absorbing member 40 is more than half, the bee can eat the poison bait agent of the present invention contained in the lower surface side which is hard to dry as compared with the upper surface 40a side which is relatively easy to dry. Therefore, it is suitable.
Although the specific embodiment of the bee poison bait container 1 has been described above with reference to FIGS. 1 and 2, the size, shape, arrangement, number, etc. are not limited to this, and may be appropriately changed according to the purpose. it can.

Hereinafter, the present invention will be described in more detail with reference to test sample preparation examples, test examples, and the like, but the present invention is not limited to these examples.
In the examples, unless otherwise specified, parts mean parts by weight.

<Aptitude confirmation test 1 for component (B)>
(1) Preparation of test sample Dissolve fipronil in various solvents, select a solvent with a solubility of fipronil of 10 g / L or more, and select diethylene glycol monoethyl ether, ethyl lactate, N-methylpyrrolidone, polyethylene glycol 600 from among them. Was selected.
As shown in Table 1 below, test samples 1 to 4 were prepared from attracting components (sugar, lactic acid bacteria beverage, etc.), the above solvent, and water. In addition, a comparative test sample containing only the attracting component without containing the above solvent and fipronil was prepared. Test Specimens 1 to 4 were liquids in which fipronil was uniformly dispersed.
The compositions of test samples 1 to 4 and comparative test samples are summarized in Table 1 below. The numerical values in Table 1 mean% by weight.

(2) Test method 25 g each of the test samples 1 to 4 and the comparative test samples whose compositions are shown in Table 1 are impregnated into a cylindrical non-woven fabric (diameter 50 mm × thickness 10 mm) and placed on a KP cup (diameter 130 mm × height 100 mm). Placed. One test worm (hornet) was put into this and observed for 45 minutes, and the average value of the eating time (seconds) per eating time was measured. Table 2 below summarizes the average eating time (seconds) for each of the test samples 1 to 4 and the comparative test sample.

As shown in Table 2, the test sample 1 containing diethylene glycol monoethyl ether, which is one of the components (B) of the present invention, maintains substantially the same bee-eating property as the comparative test sample containing only the attracting component. However, it was also confirmed that the test sample 2 containing ethyl lactate, which is one of the components (B) of the present invention, has a bee-eating property higher than that of the comparative test sample containing only the attracting component.
On the other hand, the test specimens 3 and 4 containing N-methylpyrrolidone or polyethylene glycol 600, which is a solvent other than the component (B) of the present invention, were bee-eating as compared with the comparative test sample containing only the attractant component. It was also confirmed that the sex was extremely deteriorated.
From this result, the bee poison bait of the present invention enhances the solubility of fipronil having a high insecticidal effect by blending the solvent of the specific component (B), and is equivalent to or equal to that containing only the attracting component. It was clarified that it has the above bee eating habits.

<Aptitude confirmation test 2 for component (B)>
(1) Preparation of test sample Fipronil was dissolved in various surfactants, surfactants having a fipronil solubility of 10 g / L or more were selected, and polyoxyethylene (60) cured castor oil was selected from them. ..
As shown in Table 3 below, test sample 5 was prepared from an attractant component (sugar, lactic acid bacteria beverage, etc.), the above-mentioned surfactant, fipronil, and water. In addition, the comparative test sample of the above-mentioned "Aptitude confirmation test 1 of component (B)" was used. Test sample 5 was a liquid in which fipronil was uniformly dispersed.
The compositions of test sample 5 and comparative test sample are summarized in Table 3 below. The numerical values in Table 3 mean% by weight.

(2) Test method 25 g each of the test sample 5 and the comparative test sample whose composition is shown in Table 3 was impregnated into a cylindrical non-woven fabric (diameter 50 mm × thickness 10 mm) and placed on a KP cup (diameter 130 mm × height 100 mm). .. One test worm (hornet) was put into this and observed for 45 minutes, and the average value of the eating time (seconds) per eating time was measured. For the test sample 5 and the comparative test sample, the average eating time (seconds) is shown in Table 4 below.

As shown in Table 4, the test sample 5 containing polyoxyethylene hydrogenated castor oil, which is one of the components (B) of the present invention, maintains the same bee-eating property as the comparative test sample containing only the attracting component. It was confirmed that it could be done.
From this result, the bee poison bait of the present invention is equivalent to the one containing only the attracting component while enhancing the solubility of fipronil having a high insecticidal effect by blending the surfactant of the specific component (B). It was revealed that the bee's eating habits were maintained.

<Aptitude confirmation test 3 for component (B)>
(1) Preparation of test sample Fipronil is dissolved in various solvents or surfactants, a solvent or surfactant having a fipronyl solubility of 10 g / L or more is selected, and ethyl lactate, methyl lactate, and butyl lactate are selected from the solvents. , Polyethylene glycol monolaurate (12EO), polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene lauryl ether, polyglyceryl monolaurate, polyoxyethylene sorbitan monolaurate, and polyoxyethylene sorbitan monooleate were selected from the surfactants.
As shown in Table 5 below, test samples 6 to 13 were prepared from attracting components (sugar, lactic acid bacteria beverage, etc.), the above solvent or surfactant, fipronil, and water. In addition, the test sample 2 and the comparative test sample of the above-mentioned "Aptitude confirmation test 1 of the component (B)" were used. Test Specimens 2, 6 to 13 were liquids in which fipronil was uniformly dispersed.
The compositions of test samples 2 and 6 to 13 and the comparative test samples are summarized in Table 5 below. The numerical values in Table 5 mean% by weight.

(2) Test method 25 g of each of the test samples 2 and 6 to 13 whose composition is shown in Table 5 and the comparative test sample are impregnated into a cylindrical non-woven fabric (diameter 50 mm × thickness 10 mm), and a KP cup (diameter 130 mm × height 100 mm). It was placed in. One testworm (paper wasp) was put into this and observed for 45 minutes, and the average value of the eating time (seconds) per eating time was measured. Table 6 below summarizes the average eating time (seconds) of each of the test samples 2 and 6 to 13 and the comparative test sample.

As shown in Table 6, a test sample containing ethyl lactate, polyethylene glycol monolaurate, polyglyceryl monolaurate, polyoxyethylene sorbitan monolaurate, and polyoxyethylene sorbitan monooleate, which are one of the components (B) of the present invention. It was confirmed that 2, 8, 11, 12, and 13 can maintain or improve the bee eating habits equivalent to those of the comparative test sample containing only the attracting component. On the other hand, methyl lactate and butyl lactate which are solvents which are not the component (B) of the present invention, polyoxyethylene polyoxypropylene alkyl ether and polyoxyethylene lauryl which are surfactants which are not the component (B) of the present invention. It was also confirmed that the test samples 6, 7, 9, and 10 containing ether had significantly worse bee eating habits than the comparative test samples containing only the attracting component.
From this result, the bee poison bait of the present invention enhances the solubility of fipronil having a high insecticidal effect by blending the solvent or surfactant of the specific component (B), and contains only the attracting component. It was revealed that the bee eating habits equal to or higher than those of the same were maintained.

<Condition effect confirmation test of honey-containing sugar and / or honey 1>
(1) Preparation of test sample Test sample 14 to be prepared by the attractant component (powdered sugar) not contained in the component (C) or the component (C) shown in Table 7 below, and the components (A), (B) and water. 19 was made. Test specimens 14 to 19 were liquids in which fipronil was uniformly dispersed.
The compositions of test specimens 14 to 19 are summarized in Table 7 below. The numerical values in Table 7 mean% by weight.
The following components (C) and powdered sugar in Table 7 were used.
Brown sugar: Burned brown sugar (manufactured by Ueno Sugar Co., Ltd.)
Maple Sugar: Maple Sugar Powder (manufactured by Maple Farms Japan Co., Ltd.)
Honey: Pure honey (made by TOPVALU)
Powdered sugar: Powdered sugar first snow (manufactured by Ueno Sugar Co., Ltd.)

(2) Test method 300 g of each of the test samples 14 to 19 was placed in a transparent container (made of polyethylene terephthalate), and a weather resistance acceleration test was conducted under the following conditions using a xenon weather meter X25 (manufactured by Suga Test Instruments Co., Ltd.).
Irradiance: 60 W / m ²
Tank temperature: 48 ° C
BPT (black panel) temperature: 63 ° C
Humidity: 50% RH
Irradiation time: 20 hours The fipronil concentration of test samples 14 to 19 before and after the weather resistance promotion test was measured by liquid chromatography (SIL-20A: manufactured by Shimadzu Corporation), and the reduction rate of fipronil by the weather resistance promotion test is as follows. It was calculated by the formula and is shown in Table 8 below.
[a formula]
Rate of decrease (%) = (concentration of fipronil before the start of the test-concentration of fipronil after the test) ÷ concentration of fipronil before the start of the test x 100

As shown in Table 8, it was clarified that honey-containing sugars such as brown sugar and maple sugar and honey, when used in combination with fipronil, significantly suppress the decrease in fipronil due to decomposition and oxidative action as compared with powdered sugar. It was.
That is, it was confirmed that honey-containing sugar and / or honey exerts a function as a decomposition inhibitor of fipronil.

<Condition effect confirmation test 2 of sugar-containing sugar and / or honey>
(1) Preparation of test sample Ingredients (C) brown sugar (burned brown sugar, manufactured by Ueno Sugar Co., Ltd.) shown in Table 9 below, powdered sugar (powdered sugar) which is an attracting ingredient not included in the ingredient (C) of the present invention. Test samples 20 to 23 were prepared from Hatsuyuki, manufactured by Ueno Sugar Co., Ltd.), components (A), (B) and water. Test specimens 20 to 23 were liquids in which fipronil was uniformly dispersed.
The compositions of test specimens 20 to 23 are summarized in Table 9 below. The numerical values in Table 9 mean% by weight.

(2) Test Method Using the test samples 20 to 23, a weather resistance promotion test was conducted under the same method and conditions as in the above-mentioned "Test 1 for confirming the combination effect of sugar-containing sugar and / or honey".
The fipronil concentration of the test samples 20 to 23 before and after the weather resistance promotion test was measured by liquid chromatography (SIL-20A: manufactured by Shimadzu Corporation), and the reduction rate of fipronil by the weather resistance promotion test was calculated by the following formula. , Shown in Table 10 below.
[a formula]
Rate of decrease (%) = (concentration of fipronil before the start of the test-concentration of fipronil after the test) ÷ concentration of fipronil before the start of the test x 100

As shown in Table 10, when honey and honey-containing sugar such as brown sugar are used in combination with fipronil, by adding 1% by weight or more of the total amount of poisonous bait for bees, the reduction due to decomposition of fipronil is effective. It became clear that it was suppressed.
The test sample 18 of the above-mentioned "test 1 for confirming the compounding effect of honey-containing sugar and / or honey" does not contain the component (C) of the present invention and contains 65% by weight of powdered sugar, but has a fipronil reduction rate. Considering that was 17%, the fipronil reduction rate of test sample 23, which is a composition in which 1% by weight of powdered sugar of test sample 18 was replaced with brown sugar, was 2.1%, which was found in the whole honey bait for bees. On the other hand, it has been clarified that an extremely excellent effect is exhibited by containing 1% by weight or more of the component (C) of the present invention.

<Insecticidal effect confirmation test>
A test was conducted to confirm the insecticidal effect of the bee poison bait of the present invention on pests other than bees.
(1) Test Specimen The test sample 14 of the above-mentioned "Test 1 for confirming the combination effect of sugar-containing sugar and / or honey" was used.
(2) Test method 1: An ant KP cup (diameter 80 mm x height 45 mm) was impregnated with 15 g of water impregnated and 15 g of test sample 14 in a quartet of 80 mm x 80 mm absorbent cotton. Two test containers containing 10 test insects (Amemi ants) and two comparative test containers containing only cotton wool impregnated with the test sample 14 were prepared on which the test specimens were placed.
One day after putting 10 test insects (Amemi ants) in a container, the number of lethal numbers of test insects was confirmed, and the mortality rate (%) was calculated from the average value, which is shown in Table 11 below.
(3) Test method 2: A cockroach bat (270 mm × 380 mm × height 180 mm) folds 80 mm × 80 mm cotton wool in four, impregnated with 15 g of water and impregnated with 15 g of test sample 14. Place the container (KP cup: diameter 50 mm x height 35 mm) and the paper (bellows fold) shelter (230 mm x 320 mm) in which the test insects (cockroach, male 25) are placed. Two test bats containing 50 heads and 25 females) and two comparative test bats containing only cotton wool impregnated with test sample 14 were prepared.
One day after placing 50 test insects (Smokybrown cockroaches) in the bat, the number of lethal cases of the test insects was confirmed, and the mortality rate (%) was calculated from the average value, which is shown in Table 11 below.

As shown in Table 11, it was clarified that the bee poison bait of the present invention has a high extermination effect on ants and cockroaches.
From this result, it was confirmed that the poison bait for bees of the present invention can maintain high eating habits against pests other than bees, and thus exerts a high extermination effect against pests.
It was confirmed that the test sample 14 which is the poison bait for bees of the present invention can also obtain high eating habits for bees.

Since the bee poison bait of the present invention contains fipronil having a high insecticidal effect as the component (A), in addition to obtaining a high bee extermination effect, the solubility of fipronil is enhanced as the component (B), and Since a solvent and / or a surfactant having a bee-eating property equal to or higher than that containing only an attracting component is blended, a high extermination effect on bees can be exhibited, which is very useful.
In addition, the combined use of fipronil with honey-containing sugar and / or honey can suppress the decrease in fipronil due to decomposition and the like, so that the long-term storage stability of the poison bait for bees of the present invention is improved and a high extermination effect on bees is achieved. Can be maintained for a long period of time.

Claims

Bee poison bait containing the following component (A) and the following component (B):
Ingredient (A): fipronil,
Ingredient (B): One or more selected from diethylene glycol monoethyl ether, ethyl lactate, polyoxyethylene hydrogenated castor oil, polyglyceryl monolaurate, polyoxyethylene sorbitan fatty acid ester, and polyethylene glycol monolaurate.
The bee poison bait according to claim 1, further comprising the following component (C):
Ingredient (C): sugar-containing sugar and / or honey.
A poison bait for bees containing fipronil and honey-containing sugar and / or honey.