WO2021030153A3 - Engineered t cell receptors and uses thereof - Google Patents

Engineered t cell receptors and uses thereof Download PDF

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Publication number
WO2021030153A3
WO2021030153A3 PCT/US2020/045250 US2020045250W WO2021030153A3 WO 2021030153 A3 WO2021030153 A3 WO 2021030153A3 US 2020045250 W US2020045250 W US 2020045250W WO 2021030153 A3 WO2021030153 A3 WO 2021030153A3
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WO
WIPO (PCT)
Prior art keywords
engineered
cell receptors
tcr
domain
intracellular
Prior art date
Application number
PCT/US2020/045250
Other languages
French (fr)
Other versions
WO2021030153A2 (en
Inventor
Julyun OH
Chang Yi
Dora Toledo WARSHAVIAK
Jee Young MOCK
Mark DARIS
Agnes HAMBURGER
James Johnston
Carl Alexander Kamb
Aaron Martin
Original Assignee
A2 Biotherapeutics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by A2 Biotherapeutics, Inc. filed Critical A2 Biotherapeutics, Inc.
Publication of WO2021030153A2 publication Critical patent/WO2021030153A2/en
Publication of WO2021030153A3 publication Critical patent/WO2021030153A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3069—Reproductive system, e.g. ovaria, uterus, testes, prostate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70514—CD4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70517—CD8
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70521—CD28, CD152
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment

Abstract

The invention relates to fusion proteins comprising a transmembrane domain and an intracellular domain capable of providing a stimulatory signal, and engineered T cell receptors comprising same. The disclosure provides, in part, engineered T cell receptors (TCR) comprising one or more signaling domain(s) fused to the intracellular portion of at least one TCR subunit.
PCT/US2020/045250 2019-08-09 2020-08-06 Engineered t cell receptors and uses thereof WO2021030153A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962885023P 2019-08-09 2019-08-09
US62/885,023 2019-08-09

Publications (2)

Publication Number Publication Date
WO2021030153A2 WO2021030153A2 (en) 2021-02-18
WO2021030153A3 true WO2021030153A3 (en) 2021-03-25

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2020/045250 WO2021030153A2 (en) 2019-08-09 2020-08-06 Engineered t cell receptors and uses thereof

Country Status (1)

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WO (1) WO2021030153A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2020401315B2 (en) 2019-12-11 2023-11-09 A2 Biotherapeutics, Inc. LILRB1-based chimeric antigen receptor
US20230330227A1 (en) 2020-08-13 2023-10-19 A2 Biotherapeutics, Inc. Gene fusions for control of genetically modified cells
MX2023002017A (en) 2020-08-20 2023-04-28 A2 Biotherapeutics Inc Compositions and methods for treating ceacam positive cancers.
CA3188862A1 (en) 2020-08-20 2022-02-24 Carl Alexander Kamb Compositions and methods for treating mesothelin positive cancers
WO2022040444A1 (en) 2020-08-20 2022-02-24 A2 Biotherapeutics, Inc. Compositions and methods for treating egfr positive cancers
IL304857A (en) 2021-02-16 2023-10-01 A2 Biotherapeutics Inc Compositions and methods for treating her2 positive cancers
EP4323387A2 (en) * 2021-04-13 2024-02-21 Wugen, Inc. Improved chimeric antigen receptors and uses thereof

Citations (10)

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Publication number Priority date Publication date Assignee Title
WO2010039899A2 (en) * 2008-09-30 2010-04-08 University Of Southern California Method for monitoring intracellular tyrosine phosphatase activity
US20130266551A1 (en) * 2003-11-05 2013-10-10 St. Jude Children's Research Hospital, Inc. Chimeric receptors with 4-1bb stimulatory signaling domain
US20160311901A1 (en) * 2013-07-29 2016-10-27 Bluebird Bio, Inc. Multipartite signaling proteins and uses thereof
WO2017096327A2 (en) * 2015-12-03 2017-06-08 Juno Therapeutics, Inc. Compositions and methods for reducing immune responses against cell therapies
US20180010095A1 (en) * 2016-09-23 2018-01-11 Adaptimmune Limited T Cells with Increased Immunosuppression Resistance
WO2018067993A1 (en) * 2016-10-07 2018-04-12 TCR2 Therapeutics Inc. Compositions and methods for t-cell receptors reprogramming using fusion proteins
WO2018193231A1 (en) * 2017-04-18 2018-10-25 Autolus Limited Cell
US20190022216A1 (en) * 2015-10-23 2019-01-24 Eureka Therapeutics, Inc. Antibody/t-cell receptor chimeric constructs and uses thereof
WO2019067677A1 (en) * 2017-09-29 2019-04-04 Cell Design Labs, Inc. Methods of making bispecific anti-cd307e and anti-bcma chimeric antigen receptors and uses of the same
US20190240304A1 (en) * 2016-10-20 2019-08-08 Celgene Corporation Cereblon-based heterodimerizable chimeric antigen receptors

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130266551A1 (en) * 2003-11-05 2013-10-10 St. Jude Children's Research Hospital, Inc. Chimeric receptors with 4-1bb stimulatory signaling domain
WO2010039899A2 (en) * 2008-09-30 2010-04-08 University Of Southern California Method for monitoring intracellular tyrosine phosphatase activity
US20160311901A1 (en) * 2013-07-29 2016-10-27 Bluebird Bio, Inc. Multipartite signaling proteins and uses thereof
US20190022216A1 (en) * 2015-10-23 2019-01-24 Eureka Therapeutics, Inc. Antibody/t-cell receptor chimeric constructs and uses thereof
WO2017096327A2 (en) * 2015-12-03 2017-06-08 Juno Therapeutics, Inc. Compositions and methods for reducing immune responses against cell therapies
US20180010095A1 (en) * 2016-09-23 2018-01-11 Adaptimmune Limited T Cells with Increased Immunosuppression Resistance
WO2018067993A1 (en) * 2016-10-07 2018-04-12 TCR2 Therapeutics Inc. Compositions and methods for t-cell receptors reprogramming using fusion proteins
US20190240304A1 (en) * 2016-10-20 2019-08-08 Celgene Corporation Cereblon-based heterodimerizable chimeric antigen receptors
WO2018193231A1 (en) * 2017-04-18 2018-10-25 Autolus Limited Cell
WO2019067677A1 (en) * 2017-09-29 2019-04-04 Cell Design Labs, Inc. Methods of making bispecific anti-cd307e and anti-bcma chimeric antigen receptors and uses of the same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
COURTNEY ADAM H., LO WAN-LIN, WEISS ARTHUR: "TCR SIGNALING: MECHANISMS OF INITIATION AND PROPAGATION", TRENDS IN BIOCHEMICAL SCIENCES, vol. 43, no. 2, February 2018 (2018-02-01), pages 108 - 123, XP055805438, DOI: 10.1016/j.tibs.2017.11.008 *

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