WO2021024264A2 - Anti-viral central memory cd8+ veto cells in haploidentical stem cell transplantation - Google Patents

Anti-viral central memory cd8+ veto cells in haploidentical stem cell transplantation Download PDF

Info

Publication number
WO2021024264A2
WO2021024264A2 PCT/IL2020/050865 IL2020050865W WO2021024264A2 WO 2021024264 A2 WO2021024264 A2 WO 2021024264A2 IL 2020050865 W IL2020050865 W IL 2020050865W WO 2021024264 A2 WO2021024264 A2 WO 2021024264A2
Authority
WO
WIPO (PCT)
Prior art keywords
cells
subject
cell
population
gvhd
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2020/050865
Other languages
English (en)
French (fr)
Other versions
WO2021024264A3 (en
Inventor
Yair Reisner
Esther Bachar-Lustig
Assaf Lask
Noga Or-Geva
Rotem GIDRON BUDOVSKY
Rakefet SIDLIK MUSKATEL
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yeda Research and Development Co Ltd
University of Texas System
University of Texas at Austin
Original Assignee
Yeda Research and Development Co Ltd
University of Texas System
University of Texas at Austin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2022506965A priority Critical patent/JP2022543139A/ja
Application filed by Yeda Research and Development Co Ltd, University of Texas System, University of Texas at Austin filed Critical Yeda Research and Development Co Ltd
Priority to CN202080069269.9A priority patent/CN114466925A/zh
Priority to BR112022001988A priority patent/BR112022001988A2/pt
Priority to MX2022001578A priority patent/MX2022001578A/es
Priority to AU2020326568A priority patent/AU2020326568A1/en
Priority to US17/633,294 priority patent/US20230398214A1/en
Priority to EP20850208.8A priority patent/EP4009991A4/en
Priority to CA3149379A priority patent/CA3149379A1/en
Publication of WO2021024264A2 publication Critical patent/WO2021024264A2/en
Publication of WO2021024264A3 publication Critical patent/WO2021024264A3/en
Priority to IL290337A priority patent/IL290337A/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/20—Cellular immunotherapy characterised by the effect or the function of the cells
    • A61K40/24—Antigen-presenting cells [APC]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/66—Phosphorus compounds
    • A61K31/664—Amides of phosphorus acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/17—Monocytes; Macrophages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/20—Cellular immunotherapy characterised by the effect or the function of the cells
    • A61K40/22—Immunosuppressive or immunotolerising
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/33—Antibodies; T-cell engagers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/418—Antigens related to induction of tolerance to non-self
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4202—Receptors, cell surface antigens or cell surface determinants
    • A61K40/421—Immunoglobulin superfamily
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4202—Receptors, cell surface antigens or cell surface determinants
    • A61K40/4224—Molecules with a "CD" designation not provided for elsewhere
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4231—Cytokines
    • A61K40/4234—Interleukins [IL]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/46—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C12N5/0637—Immunosuppressive T lymphocytes, e.g. regulatory T cells or Treg
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/31—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterized by the route of administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the dose, timing or administration schedule
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2307—Interleukin-7 (IL-7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2315—Interleukin-15 (IL-15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2321—Interleukin-21 (IL-21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/1121—Dendritic cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the present invention in some embodiments thereof, relates to veto cells generated from memory T cells and, more particularly, but not exclusively, to methods of their manufacture and use in therapy for attaining a stable and long term cell or tissue transplantation.
  • T cell depleted haploidentical HCT may be used, which is free of GVHD risk even in the absence of post-transplant immune suppressive therapy.
  • NMA non-myeloablative
  • One approach used to address this challenge is the use of high dose CY shortly following transplant (discussed in PCT publication nos. WO 2013/093920 and WO 2013/093919).
  • various approaches have been contemplated for generation of tolerance inducing cells (e.g. veto cells) devoid of GVH reactivity and the use of same as an adjuvant treatment for graft transplantation, some are summarized infra.
  • PCT Publication No. WO 2001/49243 discloses the use of non-alloreactive anti-third party cytotoxic T-lymphocytes (CTLs), wherein the non-alloreactive anti-third party CTLs are generated by directing T-lymphocytes of the donor against a third party antigen or antigens (in the absence of exogenous IL-2), the dose being substantially depleted of T-lymphocytes (e.g. CD4 + T cells and/or CD56 + natural killer cells) capable of developing into alloreactive CTLs.
  • CTLs non-alloreactive anti-third party cytotoxic T-lymphocytes
  • PCT Publication No. WO 2007/023491 discloses the use of tolerogenic cells for reducing or preventing graft rejection of a non-syngeneic graft in a subject.
  • the tolerogenic T regulatory cells disclosed e.g. CD4 + CD25 + cells
  • the graft e.g. bone marrow
  • the graft may be derived from any graft donor who is allogeneic or xenogeneic with the subject.
  • WO 2010/049935 discloses the use of non-GVHD inducing anti-third party cells having a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance- inducing cells and capable of homing to the lymph nodes following transplantation.
  • Tcm central memory T-lymphocyte
  • the cells are generated by contacting non-syngeneic peripheral blood mononuclear cells (PBMC) with a third party antigen or antigens in a culture deprived of cytokines and then culturing the cells in the presence of IL- 15 under conditions which allow proliferation of cells comprising the Tcm phenotype.
  • PBMC peripheral blood mononuclear cells
  • WO 2012/032526 discloses the use of non-GVHD inducing anti-third party cells having a Tcm phenotype, the cells being tolerance-inducing cells and capable of homing to the lymph nodes following transplantation, in disease treatment.
  • the cells are generated by contacting PBMCs with a third party antigen or antigens in the presence or absence of IL-21 under conditions which allow elimination of GVH reactive cells (e.g. culturing for 1-5 days) and then culturing the cells in the presence of IL- 15 under conditions which allow proliferation of cells comprising the Tcm phenotype.
  • WO 2013/035099 discloses the use of non-GVHD inducing anti-third party cells having a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance- inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation.
  • the cells are generated by contacting PBMCs with a third party antigen or antigens in the presence of IL-21 so as to allow enrichment of antigen reactive cells and then culturing the cells in the presence of IL-21, IL-15 and IL-7 in an antigen free environment so as to allow proliferation of cells comprising the Tcm phenotype.
  • WO 2018/002924 discloses veto cells generated from memory T cells, methods of their manufacture and use in transplantation and in disease treatment. According to WO 2018/002924 the veto cells are non-GVHD inducing anti-third party cells having a Tcm phenotype, are tolerance-inducing cells and/or endowed with anti-disease activity, and are capable of homing to the lymph nodes following transplantation.
  • a method of generating an isolated population of non-graft versus host disease (GVHD) inducing cells comprising a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation, the method comprising:
  • PBMCs peripheral blood mononuclear cells
  • step (d) contacting the population of cells comprising the memory T cells with the antigen presenting cells loaded with the viral peptides of step (b) in the presence of IL-21 so as to allow enrichment of viral reactive memory T cells;
  • step (e) culturing the cells resulting from step (d) in the presence of IL-21, IL-15 and/or IL-7 so as to allow proliferation of cells comprising the Tcm phenotype, thereby generating the isolated population of non-GVHD inducing cells comprising the Tcm phenotype.
  • a method of generating an isolated population of non-graft versus host disease (GVHD) inducing cells comprising a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation, the method comprising:
  • T cells in the population of cells comprise at least 40 % memory T cells expressing CD45RA-CD8 + phenotype and depleted of CD4 + , CD56 + and CD45RA + expressing cells;
  • step (c) culturing the cells resulting from step (b) in the presence of IL-21, IL-15 and/or IL-7 so as to allow proliferation of cells comprising the Tcm phenotype, thereby generating the isolated population of non-GVHD inducing cells comprising the Tcm phenotype.
  • a method of generating an isolated population of non-graft versus host disease (GVHD) inducing cells comprising a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation, the method comprising:
  • PBMCs peripheral blood mononuclear cells
  • step (d) contacting the population of cells comprising the memory T cells with the dendritic cells loaded with the viral peptides of step (b) in the presence of IL-21 so as to allow enrichment of viral reactive memory T cells;
  • step (e) culturing the cells resulting from step (d) in the presence of IL-21, IL-15 and/or IL-7 so as to allow proliferation of cells comprising the Tcm phenotype, thereby generating the isolated population of non-GVHD inducing cells comprising the Tcm phenotype.
  • an isolated population of non-GVHD inducing cells comprising cells having a central memory T- lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti- disease activity, and capable of homing to the lymph nodes following transplantation, generated according to the method of some embodiments of the invention.
  • Tcm central memory T- lymphocyte
  • a pharmaceutical composition comprising as an active ingredient the isolated population of non- GVHD inducing cells of some embodiments of the invention and a pharmaceutical acceptable carrier.
  • a method of treating a disease in a subject in need thereof comprising administering to the subject a therapeutically effective amount of the isolated population of non-GVHD inducing cells of some embodiments of the invention, thereby treating the disease in the subject.
  • a method of reducing graft rejection and/or graft versus host disease (GVHD) and/or inducing donor specific tolerance in a subject in need thereof, wherein the subject is in need of a non-syngeneic cell or tissue transplant comprising administering to the subject a therapeutically effective amount of the isolated population of non-GVHD inducing cells of some embodiments of the invention, thereby reducing graft rejection and/or GVHD in the subject.
  • GVHD graft versus host disease
  • GVHD graft versus host disease
  • a method of treating a subject in need of a non-syngeneic cell or tissue transplant comprising:
  • a method of treating a subject in need of an immature hematopoietic cell transplantation comprising:
  • non-myeloablative pre-transplant conditioning protocol comprising a total body irradiation (TBI) and a chemotherapeutic agent, wherein the TBI and the chemotherapeutic agent are administered on days -7 to -1 prior to transplantation (e.g. on days -7 to -2, e.g. on days -6 to -1, prior to transplantation);
  • T cell depleted immature hematopoietic cells comprises less than 5 x 10 5 CD3 + T cells per kilogram ideal body weight of the subject, and wherein the T cell depleted immature hematopoietic cells comprise at least 5 x 10 6 CD34 + cells per kilogram ideal body weight of the subject;
  • the first population of the PBMCs and the second population of the PBMCs are from the same batch.
  • the memory T cells expressing the CD45RA-CD8 + phenotype constitute at least 40 % of T cells in the population of cells.
  • the agent is an antibody.
  • the contacting in the presence of IL-21 is affected for 12 hours to 6 days. According to some embodiments of the invention, the contacting in the presence of IL-21 is affected for 3 days.
  • the culturing in the presence of any of IL-21, IL-15 and/or IL-7 is affected for 6 to 12 days.
  • the culturing in the presence of any of IL-21, IL-15 and/or IL-7 is affected for 9 days.
  • the culturing further comprises adding glucose to a concentration of at least 50 mg/dl.
  • the viral peptides are presented on antigen presenting cells.
  • the antigen presenting cells are obtained by a method comprising contacting PBMCs from a donor subject with an antibody capable of binding CD14 + expressing cells and selecting CD14 + expressing cells capable of maturing into antigen presenting cells.
  • the antigen presenting cells are of the same donor subject as the population of cells comprising the memory T cells.
  • the selecting CD14 + expressing cells is not affected by plastic adherence.
  • the antigen presenting cells comprise dendritic cells.
  • the dendritic cells are mature dendritic cells.
  • the dendritic cells are obtained by a method comprising culturing the CD14 + expressing cells in a culture comprising dendritic cell maturation factors.
  • the viral peptides are derived from 1-10 types of viruses.
  • the viral peptides are derived from 2-10 types of viruses.
  • the viral peptides are derived from 4-10 types of viruses.
  • At least one of the viral peptides comprises at least one peptide from a BK vims.
  • the viral peptides comprise an Epstein-Barr virus (EBV) peptide, a cytomegalovirus (CMV) peptide, a BK Virus peptide and an Adenovirus (Adv) peptide.
  • EBV Epstein-Barr virus
  • CMV cytomegalovirus
  • Adv Adenovirus
  • the viral peptides comprise at least one of EBV-LMP2, EBV-BZLF1, EBV-EBNA1, EBV select, CMV-pp65, CMV-IE-1, Adv-penton, Adv- hexon, BKV LT, BKV (capsid VP1), BKV (capsid protein VP2), BKV (capsid protein VP2, isoporm VP3), BKV (small T antigen).
  • EBV-LMP2, EBV-BZLF1, EBV-EBNA1, EBV select CMV-pp65, CMV-IE-1, Adv-penton, Adv- hexon, BKV LT, BKV (capsid VP1), BKV (capsid protein VP2), BKV (capsid protein VP2, isoporm VP3), BKV (small T antigen).
  • the viral peptides comprise at least one of AdV5 Hexon, hCMV pp65, EBV select and BKV LT.
  • the viral peptides comprise AdV5 Hexon, hCMV pp65, EBV select and BKV LT.
  • the viral peptides further comprise at least one type of a bacterial peptide, a fungal peptide, or a tumor peptide.
  • the viral peptides and at least one type of a bacterial peptide, a fungal peptide, or a tumor peptide are utilized together. According to some embodiments, the viral peptides and at least one type of a bacterial peptide, a fungal peptide, or a tumor peptide are utilized separately.
  • the PBMCs are non-syngeneic with respect to a subject.
  • the PBMCs are allogeneic with respect to a subject.
  • the population of cells comprising the memory T cells is non-syngeneic with respect to a subject.
  • the population of cells comprising the memory T cells is allogeneic with respect to a subject.
  • the cells having the Tcm phenotype comprise a CD3 + , CD8 + , CD62L + , CD45RA-, CD45RO + signature.
  • the cells having the Tcm phenotype comprise at least 30 % of the isolated population of non-GVHD inducing cells.
  • the isolated population of non-GVHD inducing cells are formulated for administration as fresh cells.
  • the isolated population of non-GVHD inducing cells are formulated for administration as cryopreserved cells.
  • the method further comprises transplanting a cell or tissue transplant into the subject.
  • the medicament further comprises a cell or tissue transplant.
  • the method further comprises administering to the subject genetically modified T cells.
  • the medicament further comprises genetically modified T cells.
  • the genetically modified T cells comprise CAR-T cells or TCR- transgenic T cells.
  • the cell or tissue transplant comprises immature hematopoietic cells.
  • the disease is a malignant disease.
  • the malignant disease is a solid tumor or tumor metastasis.
  • the malignant disease is a hematological malignancy.
  • the malignant disease is selected from the group consisting of a leukemia, a lymphoma, a myeloma, a melanoma, a sarcoma, a neuroblastoma, a colon cancer, a colorectal cancer, a breast cancer, an ovarian cancer, an esophageal cancer, a synovial cell cancer, a hepatic cancer, a pancreatic cancer, and a metastasis or a relapse.
  • the disease is a non-malignant disease.
  • the non-malignant disease is selected from the group consisting of an organ dysfunction or failure, a hematologic disease, a graft related disease, an infectious disease, a genetic disease or disorder, a sickle cell disease, an autoimmune disease and a metabolic disorder.
  • the non-malignant disease is selected from the group consisting of an aplastic anemia, a severe immune deficiency and a non-malignant bone marrow failure.
  • the isolated population of non-GVHD inducing cells is for administration following the cell or tissue transplant (e.g. non-syngeneic cell or tissue transplant).
  • the cell or tissue transplant e.g. non-syngeneic cell or tissue transplant.
  • the isolated population of non-GVHD inducing cells is for administration on day 6-9 following the cell or tissue transplant (e.g. non- syngeneic cell or tissue transplant). According to some embodiments of the invention, the isolated population of non-GVHD inducing cells is for administration on day 7 following the cell or tissue transplant (e.g. non- syngeneic cell or tissue transplant).
  • the isolated population of non-GVHD inducing cells is for administration at a dose of at least 2.5 x 10 6 CD8 + cells per kg ideal body weight.
  • the non-syngeneic cell or tissue transplant comprises immature hematopoietic cells.
  • the immature hematopoietic cells comprise T cell depleted immature hematopoietic cells.
  • the immature hematopoietic cells comprise at least 5 x 10 6 CD34 + cells per kilogram ideal body weight of the subject.
  • the immature hematopoietic cells are depleted of CD3 + and/or CD19 + expressing cells. According to some embodiments of the invention, the immature hematopoietic cells comprise less than 4 x 10 5 CD3 + expressing cells per kg ideal body weight of the subject.
  • the immature hematopoietic cells comprise less than 3 x 10 5 CD3 + expressing cells per kg ideal body weight of the subject.
  • the immature hematopoietic cells comprise less than 2 x 10 5 CD3 + expressing cells per kg ideal body weight of the subject.
  • the method further comprises conditioning the subject under non-myeloablative conditioning protocol prior to the transplanting.
  • the method further comprises a non- myeloablative pre-transplant conditioning protocol.
  • the non-myeloablative conditioning protocol comprises at least one of total body irradiation (TBI), a partial body irradiation (TLI), a chemotherapeutic agent, an antibody immunotherapy or a co- stimulatory blockade.
  • the TBI comprises a single or fractionated irradiation dose within the range of 1-5 Gy. According to some embodiments of the invention, TBI is administered on day -1 prior to transplantation.
  • TBI is administered on the day of transplantation, e.g. in the morning of day 0, and transplantation is carried out on the same day, e.g. in the evening.
  • the chemotherapeutic agent comprises at least one of Fludarabine, Cyclophosphamide, Rapamycin, Busulfan, Trisulphan, Melphalan or Thiotepa.
  • the non-myeloablative conditioning protocol comprises T cell debulking.
  • the non-myeloablative conditioning protocol does not comprise T cell debulking.
  • the T cell debulking is affected by at least one of anti-thymocyte globulin (ATG) antibodies, anti-CD52 antibodies or anti-CD3 (OKT3) antibodies.
  • ATG anti-thymocyte globulin
  • OKT3 anti-CD3
  • the method further comprises administering to the subject a therapeutically effective amount of cyclophosphamide subsequent to the transplanting.
  • the therapeutically effective amount of the cyclophosphamide comprises 25-200 mg cyclophosphamide per kilogram ideal body weight of the subject.
  • the therapeutically effective amount of the cyclophosphamide is to be administered to the subject in two doses 3 and 4 days post- transplant.
  • the non-myeloablative pre-transplant conditioning protocol comprises T cell debulking.
  • the T cell debulking is affected by anti- thymocyte globulin (ATG).
  • ATG anti- thymocyte globulin
  • the non-myeloablative pre-transplant conditioning protocol does not comprise T cell debulking.
  • corticosteroids are not administered.
  • the subject is not treated chronically with GVHD prophylaxis following transplantation.
  • the subject is a human subject.
  • FIG. 1 is a flow diagram outlining processing and testing of anti-viral CD8 + veto cell depleted cells according to some embodiments of the invention.
  • FIG. 2 is a flow diagram outlining processing and testing of CD34 + enriched and CD3 + CD19 + depleted cells according to some embodiments of the invention.
  • FIG. 3 is a schematic representation of a conditioning regimen of a recipient subject with fresh immature hematopoietic cell transplant for use according to some embodiments of the invention.
  • FIG. 4 is a schematic representation of a conditioning regimen of a recipient subject with cryopreserved immature hematopoietic cell transplant for use according to some embodiments of the invention.
  • FIG. 5 is a schematic illustration of a combination protocol combining mis-matched megadose T cell depleted hematopoietic stem cell transplant with post-transplant high dose CY, following non-myeloablative conditioning by T cell debulking (with ATG), Fludarabine and 3 GY TBI.
  • FIG. 6 is a graph illustrating that infusion of veto cells on day 7 enables to overcome graft rejection in the absence of T cell debulking of the recipients.
  • C3H recipient mice were transplanted with megadose T cell depleted Balb/Nude (H2 d ) BM cells following 3.5 GY TBI.
  • Transplanted mice also received either CY post-transplant (100 mg/kg) on day 3-4 (designated Nu/BN + CTX) or 5 x 10 6 Balb/c veto Tcm (H2 d ) on day 7 post-transplant (designated Nu/BN + Tcm D-7), or both (designated Nu/BN + Tcm D7 + CTX).
  • Percentage of donor cells in peripheral blood was analyzed by FACS using anti-donor and anti-host (H2 d and H2 k ) antibodies. Each dot represents one mouse belonging to the appropriate group.
  • FIG. 7 is a schematic illustration of a treatment protocol without ATG which is replaced by administration of veto cells on day 7.
  • FIGs. 8A-C illustrate the anti- viral activity of Tcm cells as determined by intracellular staining of INF-g and TNF-a.
  • Veto-Tcm cells were co-cultured with the respective viral peptide mix (EBV, CMV, Adeno, BKV) in the presence of Brefeldin A (eBioscience) at 37 °C 5% CO2 for 6 hrs.
  • Cells were fixed, permeabilized (Invitrogen Fix & Perm set), and immunostained for CD45, CD3, CD8, IFNy, and TNFa (BD). Positive control included TCR-independent stimulation with PMA/Ionomycin.
  • Cells were gated on a CD45 + /FSC lymphocyte gate and CD3 + CD8 + .
  • the present invention in some embodiments thereof, relates to veto cells generated from memory T cells and, more particularly, but not exclusively, to methods of their manufacture and use in therapy for attaining a stable and long term cell or tissue transplantation.
  • tolerance inducing cells e.g. veto cells
  • an anti-disease activity e.g. anti-viral activity
  • GVH graft versus host
  • T cell depleted megadose allogeneic hematopoietic cell transplantation can tolerize host anti-donor T cells, enable engraftment in the absence of GVHD following safe, non-myeloablative (NMA) conditioning (e.g. comprising TBI and a chemotherapeutic agent such as Fludarabine), with minimal post-transplant immune suppressive therapy comprising cyclophosphamide (e.g. administered on days +3 and +4 post HCT).
  • NMA non-myeloablative
  • PBMC peripheral blood mononuclear cells
  • MNC mononuclear cells
  • the MNC were separated into two fractions, one for generating stimulator cells (i.e. antigen presenting cells presenting viral peptides) and the second for generating anti-viral central memory CD8 + veto cells.
  • stimulator cells were generated from the first MNC fraction by selecting CD14 + expressing cells from the MNC fraction using CD14-binding monoclonal antibodies, the CD14 + expressing cells were grown with dendritic cell maturation factors comprising IL-4, GM-CSF, IFN- g and LPS for 16 hours.
  • the mature dendritic cells were then loaded with viral peptides comprising EBV, CMV, BKV and Adenovirus, and were irradiated (e.g. by 25 Gy).
  • viral peptides comprising EBV, CMV, BKV and Adenovirus
  • memory cells were enriched from the second MNC fraction by negative selection of CD4 CD56 CD45RA- expressing cells using CD4 + , CD56 + and CD45RA + antibodies conjugated to super-paramagnetic particles.
  • the CD4 CD56 CD45RA- depleted cells were co-cultured with the viral-loaded dendritic cells (at a ratio of 5:1 cells to DC) for 3 days in a T cell growth media deprived of cytokines and supplemented with only IL-21 (such conditions allow proliferation of viral reactive memory CD8 T cells and elimination of GVH reactive cells). After 3 days, the T cells were cultured in the presence of IL-21, IL-15 and IL-7 for an additional 9 days (such conditions allow proliferation of cells comprising the Tcm phenotype). The cell medium was replaced every 2 days and the glucose and PH levels were monitored and adjusted as needed. The resulting population of cells comprise anti-viral central memory CD8 + veto cells as determined by immunophenotyping (e.g.
  • CD3 + CD8 + CD62L + CD45RO + functional immune studies (e.g. by assessing the anti-viral activity, for example, by restimulating the Tcm cells against the original viral peptides used at the beginning of the culture and measuring the percentage of INFg + TNF + cells e.g. by FACS).
  • the anti-viral central memory CD8 + veto cells facilitate engraftment of allogeneic CD34 + enriched CD3 + CD19 + depleted hematopoietic cell transplantation (i.e. megadose T cell depleted allogeneic hematopoietic stem cell (HSC) transplant) following a non-myeloablative (NMA) conditioning regimen.
  • the recipient subject may be treated daily with fludarabine on days -6 to -3 (or on days -7 to -4) followed by low dose TBI on day -1 (or on day -2) (i.e. on days 6 to 3, days 7 to 4, day 1, or day 2, respectively, prior to transplantation) as the preparative regimen.
  • the patient receives cyclophosphamide (CY) followed by the infusion of the veto cells on day +7 (i.e. day +7 after transplantation).
  • CY cyclophosphamide
  • ATG can be administered daily on days on days -9 to -7 (or on days - 10 to -8) prior to transplantation so as to induce T cell debulking in the recipient subject.
  • the anti-viral central memory CD8 + veto cells offer disease treatment (e.g. protection from viral infections or treatment of viral infections) coupled with enhanced engraftment of donor hematopoietic progenitors, by virtue of their ability to induce immune tolerance towards donor cells.
  • the veto cells enable engraftment of megadose T cell depleted allogeneic HSC transplant without prolonged immune suppression following a safe non-myeloablative regimen.
  • the large scale protocol provided enables generation of anti-viral central memory CD8 + veto cells in doses suitable for use in treatment of human subjects.
  • a method of generating an isolated population of non-graft versus host disease (GVHD) inducing cells comprising a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation, the method comprising:
  • PBMCs peripheral blood mononuclear cells
  • step (d) contacting the population of cells comprising the memory T cells with the antigen presenting cells loaded with the viral peptides of step (b) in the presence of IL-21 so as to allow enrichment of viral reactive memory T cells;
  • step (e) culturing the cells resulting from step (d) in the presence of IL-21, IL-15 and/or IL-7 so as to allow proliferation of cells comprising the Tcm phenotype, thereby generating the isolated population of non-GVHD inducing cells comprising the Tcm phenotype.
  • a method of generating an isolated population of non graft versus host disease (GVHD) inducing cells comprising a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation comprising: (a) providing a population of cells comprising T cells, wherein the T cells in the population of cells comprise at least 40 % memory T cells expressing a CD45RA-CD8 + phenotype and depleted of CD4 + , CD56 + and CD45RA + expressing cells; and
  • step (c) culturing the cells resulting from step (b) in the presence of IL-21, IL-15 and/or IL-7 so as to allow proliferation of cells comprising the Tcm phenotype, thereby generating the isolated population of non-GVHD inducing cells comprising the Tcm phenotype.
  • isolated refers to cells which have been isolated from their natural environment (e.g., the human body).
  • a population of cells refers to a heterogeneous cell mixture.
  • non-graft versus host disease or “non-GVHD” as used herein refers to having substantially reduced or no graft versus host (GVH) inducing reactivity.
  • the cells of some embodiments of the present invention are generated as to not significantly cause graft versus host disease (GVHD) as evidenced by survival, weight and overall appearance of the transplanted subject 30-120 days following transplantation. Methods of evaluating a subject for reduced GVHD are well known to one of skill in the art.
  • the cells of some embodiments of the present invention have at least 10 %, at least 20 %, at least 30 %, at least 40 %, at least 50 %, at least 55 %, at least 60 %, at least 65 %, at least 70 %, at least 75 %, at least 80 %, at least 85 %, at least 90 %, at least 95 % or even 100 % reduced reactivity against a host relative to cells not generated according to the present teachings.
  • central memory T-lymphocyte (Tcm) phenotype refers to a subset of T cells which home to the lymph nodes.
  • FACS Fluorescence-activated cell sorting
  • cells having the Tcm phenotype comprise 20 % of the isolated population of non-GVHD inducing cells.
  • cells having the Tcm phenotype comprise 30 % of the isolated population of non-GVHD inducing cells.
  • cells having the Tcm phenotype comprise 40 % of the isolated population of non-GVHD inducing cells.
  • cells having the Tcm phenotype comprise 50 % of the isolated population of non-GVHD inducing cells.
  • cells having the Tcm phenotype comprise 60 % of the isolated population of non-GVHD inducing cells.
  • cells having the Tcm phenotype comprise 70 % of the isolated population of non-GVHD inducing cells.
  • about 10-20 %, about 10-30 %, about 10-40 %, about 10-50 %, about 20-30 %, about 20-40 %, about 30-50 %, about 40-60 %, about 50-70 %, about 60- 80 %, about 70-90 %, about 80-100 %, or about 90-100 % of the isolated population of non-GVHD inducing cells have the Tcm cell signature.
  • cells having the Tcm phenotype comprise 10-30 % of the isolated population of non-GVHD inducing cells.
  • cells having the Tcm phenotype comprise 10-50 % of the isolated population of non-GVHD inducing cells.
  • cells having the Tcm phenotype comprise 20-40 % of the isolated population of non-GVHD inducing cells.
  • cells having the Tcm phenotype comprise 30-50 % of the isolated population of non-GVHD inducing cells.
  • cells having the Tcm phenotype comprise 50-70 % of the isolated population of non-GVHD inducing cells.
  • Tcm cells The non-GVHD inducing cells comprising a Tcm phenotype of the invention are also referred to herein as “Tcm cells”.
  • Tcm cells typically home to the lymph nodes following transplantation.
  • the isolated population of cells of some embodiments of the present invention may home to any of the lymph nodes following transplantation, as for example, the peripheral lymph nodes and mesenteric lymph nodes.
  • the homing nature of these cells allows them to exert their veto effect in a rapid and efficient manner.
  • the non-GVHD inducing cells comprising a Tcm phenotype of some embodiments of the present invention are tolerance-inducing cells.
  • tolerance inducing cells refers to cells which provoke decreased responsiveness of the recipient's cells (e.g. recipient's T cells) when they come in contact with the donor cells as compared to the responsiveness of the recipient's cells in the absence of administered tolerance inducing cells.
  • Tolerance inducing cells include veto cells (i.e. T cells which lead to apoptosis of host T cells upon contact with same) as was previously described in PCT Publication Nos. WO 2001/049243 and WO 2002/102971.
  • veto activity relates to immune cells (e.g. donor derived T cells) which lead to inactivation of anti-donor recipient T cells upon recognition and binding to the veto cells. According to one embodiment, the inactivation results in apoptosis of the anti-donor recipient T cells.
  • immune cells e.g. donor derived T cells
  • non-GVHD inducing cells comprising a Tcm phenotype of the invention are also referred to herein as “veto cells”.
  • the isolated population of non-GVHD inducing cells comprising a Tcm phenotype of some embodiments of the present invention comprises anti-disease activity.
  • anti-disease activity refers to the function of the Tcm cells against a diseased cell.
  • the anti-disease activity may be directly against a diseased cell, e.g. killing capability of the diseased cell. This activity may be due to TCR independent killing mediated by LFA1 -I/CAM 1 binding [Arditti et al., Blood (2005) 105(8):3365-71. Epub 2004 Jul 6].
  • the anti-disease activity may be indirect, e.g. by activation of other types of cells (e.g. CD4 + T cells, B cells, monocytes, macrophages, NK cells) which leads to death of the diseased cell (e.g. by killing, apoptosis, or by secretion of other factors, e.g. antibodies, cytokines, etc.).
  • anti-viral activity refers to the function of the Tcm cells against a virally infected cell (e.g. expressing viral antigen/s in the context of MHC-peptide complex on the cell surface). Typically the anti-viral activity results in killing of the virally infected cell.
  • anti-tumor activity refers to the function of the Tcm cells against a tumor cell. Typically the anti-tumor activity results in killing of the tumor cell. According to a specific embodiment, anti-tumor activity comprises graft versus leukemia/lymphoma (GVL) activity.
  • VTL leukemia/lymphoma
  • a diseased cell may comprise, for example, a virally infected cell, a bacterial infected cell, a cancer cell [e.g. cell of a solid tumor or leukemia/lymphoma cell, also referred to herein as graft versus leukemia (GVL) activity of the Tcm cells], a cell associated with an autoimmune disease, a cell associated with an allergic response, or a cell altered due to stress, radiation or age.
  • the non-GVHD inducing cells of some embodiments of the present invention comprising a Tcm phenotype may be non-genetically modified cells or genetically modified cells (e.g.
  • the Tcm cells express a chimeric antigen receptor (CAR) or a modified T cell receptor (TCR).
  • CAR chimeric antigen receptor
  • TCR modified T cell receptor
  • the Tcm cells of some embodiments of the invention may be transduced to express a TCR or a CAR.
  • TCR T cell receptor
  • the TCR comprises the variable region of a TCR (e.g. a- and b-chains or g- and d-chains).
  • Method of transducing cells (e.g. T cells) with a TCR are known in the art and are disclosed e.g. in Nicholson et al. Adv Hematol. 2012; 2012:404081; Wang and Riviere Cancer Gene Ther. 2015 Mar;22(2):85-94); and Lamers et al., Cancer Gene Therapy (2002) 9, 613-623.
  • transduction with a CAR refers to cloning of a nucleic acid sequence encoding a chimeric antigen receptor (CAR), wherein the CAR comprises an antigen recognition moiety and a T-cell activation moiety.
  • a chimeric antigen receptor (CAR) is an artificially constructed hybrid protein or polypeptide containing an antigen binding domain of an antibody (e.g., a single chain variable fragment (scFv)) linked to T-cell signaling or T-cell activation domains.
  • the genetically modified Tcm cells express a transgenic TCR (TCR-T) or a CAR (CAR-T).
  • the isolated population of non-GVHD inducing cells comprising a Tcm phenotype of some embodiments of the invention may be genetically modified for therapy (e.g. anti-cancer cell therapy) by redirecting the cell specificity (e.g. T cell specificity) by promoting presentation of a receptor targeting a disease antigen (e.g. tumor antigen) by way of transducing with a T cell receptor (TCR) or a chimeric antigen receptor (CAR).
  • TCR or CAR expressing Tcm cells may be generated to target malignant diseases e.g.
  • CD20 or GD2 follicular lymphoma
  • CD171 follicular lymphoma
  • CD20 non-Hodgkin lymphoma
  • CD19 lymphoma
  • IL13Ra2 glioblastoma
  • CLL chronic lymphocytic leukemia
  • ALL acute lymphocytic leukemia
  • a method of generating the isolated population of non-GVHD inducing cells comprising a Tcm phenotype.
  • the method comprises contacting a first population of peripheral blood mononuclear cells (PBMCs) from a donor subject with an antibody capable of binding CD14 + expressing cells and selecting CD14 + expressing cells capable of maturing into antigen presenting cells.
  • PBMCs peripheral blood mononuclear cells
  • donor subject refers to a human being.
  • the donor subject is non-syngeneic with respect to a subject (e.g. allogeneic), as discussed in detail hereinbelow.
  • PBMCs peripheral blood mononuclear cells
  • the PBMCs are non-syngeneic with respect to a recipient subject (e.g. allogeneic), as discussed in detail hereinbelow.
  • the PBMCs used for generation of the non-GVHD inducing cells comprising the Tcm phenotype are non-mobilized (i.e. unprimed) PBMCs, i.e. cells not obtained by means of using drugs to affect the movement of hematopoietic precursors (e.g., stem cells) from bone marrow into peripheral blood circulation.
  • hematopoietic precursors e.g., stem cells
  • the PBMCs are collected 5-15 days (e.g. 7-10 days, e.g. 7 days, e.g. 8 days) prior to the planned transplant date of the hematopoietic cells (HCT) (i.e. Day 0), as discussed in detail hereinbelow.
  • HCT hematopoietic cells
  • PBMCs can be collected at any time prior to the planned transplant date.
  • Such PBMCs can be stored as is, or can be treated as discussed below and then stored for future use (e.g. cryopreserved).
  • PBMCs are collected from a donor subject using standard techniques.
  • PBMCs are collected using leukapheresis, i.e. a process which essentially removes PBMCs from a donor subject, returning the remaining blood components to the donor subject.
  • the PBMC collection procedure (e.g. leukapheresis) yields e.g. 0.01-1000 x 10 10 mononuclear cells (MNC), e.g. 0.1-100 x 10 10 , e.g. 1 x 10 10 MNC.
  • the PBMC collection procedure (e.g. leukapheresis) yields a minimum of 500 x 10 6 mononuclear cells (MNC), e.g. 500 x 10 10 to 1000 x 10 10 MNC, e.g. 1 x 10 10 MNC.
  • the PBMCs are collected in a single collection procedure.
  • the PBMCs are collected in a two, three, four, five or more collection procedure (e.g. in order to obtain the required number of MNC).
  • the PBMCs may be pooled together (e.g. for further processing) or used separately.
  • any of the aformentioned collections may be referred to as a batch.
  • any group of collections e.g. from the same donor subject in the context of collection over several days e.g. 1, 2, 3, 4, 5 days, e.g. 3 days
  • any of the aformentioned collections of PBMCs may be kept in a collection tube for one or more days (e.g. e.g. 1, 2, 3, 4, 5 days, e.g. 1-2 days) prior to further processing (e.g. MNC isolation as discussed below).
  • days e.g. 1, 2, 3, 4, 5 days, e.g. 1-2 days
  • further processing e.g. MNC isolation as discussed below.
  • MNC mononuclear cells
  • the collected PBMCs are diluted (e.g. at 1:2) with Dulbecco’s Phosphate-Buffered Saline (DPBS), e.g. without Calcium and Magnesium and e.g. supplemented with e.g. 0.6 % ACD-A and 0.5 % of 25 % HAS)), and the MNC are isolated by ficoll density gradient separation.
  • the MNC preparation of one embodiment is platelet washed (i.e. thrombowashed), e.g. 1-5 times, e.g. 1-3 times e.g. twice, by manual centrifugation and is resuspended with Wash Buffer (e.g. PBS with ACD-A and 0.5 % of 25 % HSA).
  • the PBMC preparation or MNC preparation of some embodiments of the invention is divided into two fractions (e.g. equal fractions).
  • One PBMC or MNC fraction is further processed into antigen presenting cells (i.e. referred to herein as first population of PBMCs) and the second PBMC or MNC fraction (i.e. referred to herein second population of PBMCs) is enriched for CD8 + memory T cells.
  • the first population of PBMCs and the second population of PBMCs are from the same batch.
  • two PBMC preparations may be used from different PBMC collection procedures.
  • the first population of PBMCs and the second population of PBMCs are from diverse batches.
  • antigen presenting cells e.g. dendritic cells
  • the antibody capable of binding CD14 + expressing cells is a CD14 monoclonal antibody.
  • CD14 monoclonal antibodies can be obtained commercially e.g. from BD Biosciences, Santa Cruz Biotechnology and R&D Systems.
  • Selecting CD14 + expressing cells using CD14 monoclonal antibodies may be carried out using any method known in the art, such as by the use of magnetic-activated cell sorting (MACSTM) available from e.g. Miltenyi Biotec, FACS sorter and/or capture ELISA labeling.
  • MCSTM magnetic-activated cell sorting
  • different depletion/separation methods can be combined, for example, magnetic cell sorting can be combined with FACS, to increase the separation quality or to allow sorting by multiple parameters.
  • selection of CD14 + expressing cells is not affected by plastic adherence.
  • the CD14 + expressing cells are selected by magnetic separation techniques.
  • Different magnetic beads are available from a number of sources, including for example, Dynal (Norway), Advanced Magnetics (Cambridge, MA, U.S.A.), Immuncon (Philadelphia, U.S.A.), Immunotec (Marseille, France), Invitrogen, Stem cell Technologies (U.S.A), Cellpro (U.S.A) and Miltenyi Biotec GmbH (Germany).
  • antibodies can be biotinylated or conjugated with digoxigenin and used in conjunction with avidin or anti- digoxigenin coated affinity columns.
  • the CD14 monoclonal antibodies are conjugated to magnetic particles.
  • the magnetic particles comprise super-paramagnetic iron dextran particles.
  • the CD14 labeled cells are processed on CliniMACS ® column.
  • the CD14 labeled cells are selected on SuperMACSTM II using the XS Separation Column.
  • the CD14 magnetically labeled cells i.e. CD14 + expressing cells
  • the separation column i.e. positive selection
  • the CD14 + cells are then released from the column and collected.
  • samples from each fraction are removed for cell count, viability and/or immunopheno typing .
  • viability is assessed by positive expression of 7AAD, i.e. 7AAD + cells.
  • the CD14 + enriched cell preparation is resuspended at a cell concentration of e.g. 1-10 x 10 6 cells/ml, e.g. 3 x 10 6 cells/ml, in cell culture medium (e.g. dendritic cell culture medium, e.g. CellGro/1 % HSA).
  • cell culture medium e.g. dendritic cell culture medium, e.g. CellGro/1 % HSA.
  • the cell culture medium is supplemented with cytokines and growth factors. Determination of cytokines and growth factors to be used is within the skill of a person of skill in the art.
  • the cell culture medium is supplemented with IL-4 (e.g. 200-2000 IU/mL, e.g. 1000 IU/mL) and GM-CSF (e.g.
  • the cell suspension is then seeded (e.g. in cell culture plates e.g. Cell Factory plates) and incubated for 12-36 hours, e.g. for 16-24 hours, e.g. for 24 hours, in at 37 °C, 5 % CO 2 .
  • the CD14 + enriched cell preparation is cultured in the presence of maturation factors (e.g. dendritic cell maturation factors). Determination of maturation factors to be used is within the skill of a person of skill in the art.
  • the seeded (e.g. in cell culture plates, e.g. Cell Factory plates) CD14 + enriched cells are cultured in the presence of IL-4 (e.g. 200-2000 IU/mL, e.g. 1000 IU/mL), GM-CSF (e.g. 1000- 4000 IU/mL, e.g.
  • LPS e.g. 10-100 ng/mL, e.g. 40 ng/mL
  • IFN-g e.g. 50-500 IU/mL, e.g. 200 IU/mL
  • 10-24 hours e.g. for 14-18 hours, e.g. for 16 hours, in at 37 °C, 5 % CO 2 .
  • the antigen presenting cells e.g. mature dendritic cells i.e. mDCs
  • the antigen presenting cells are obtained from the cell culture.
  • non-adherent cells are removed and the antigen presenting cells (i.e. adherent cells) are detached from the culture plates using any method known in the art (e.g. by adding ice-cold buffer e.g. ACD-A with 0.5 % of 25 % HAS and DPBS Buffer and incubation on ice or frozen gel packs for 10-60 minutes, e.g. 30 minutes).
  • the harvested antigen presenting cells are then centrifuged, washed and resuspended (e.g. in ACD-A with 0.5 % of 25 % HAS and DPBS Buffer).
  • antigen presenting cells e.g. mDCs
  • an antigen or antigens are loaded with an antigen or antigens.
  • loading refers to the attachment of an antigen or antigens (e.g. peptides) to MHC peptides (e.g. MHC class I or II) presented in the peptide-MHC complex on the surface of the antigen-presenting cell (APC).
  • an antigen or antigens e.g. peptides
  • MHC peptides e.g. MHC class I or II
  • APC antigen-presenting cell
  • an antigen or antigens refers to a soluble or non-soluble (such as membrane associated) molecule capable of inducing an immune response.
  • an antigen or antigens can be whole cells (e.g. live or dead cells), cell fractions (e.g. lysed cells), cell antigens (e.g. cell surface antigens), a protein extract, a purified protein or a synthetic peptide.
  • the antigen or antigens comprise viral antigens.
  • the viral antigens are derived from at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20 or more different types of viruses.
  • the viral antigens are derived from 1-50, 1-40, 1-30, 1-25, 1- 20, 1-15, 1-10, 1-9, 1-8, 1-7, 1-6, 1-5, 1-4, 1-3, 1-2, 2-50, 2-40, 2-30, 2-20, 2-10, 2-8, 2-6, 2-4, 3- 50, 3-40, 3-30, 3-20, 3-10, 3-9, 3-7, 3-5, 3-4, 4-50, 4-40, 4-30, 4-20, 4-10, 4-8 or 4-6 types of viruses.
  • the viral antigens are derived from 1-20 types of viruses.
  • the viral antigens are derived from 1-10 types of viruses.
  • the viral antigens are derived from 1-4 types of viruses.
  • the viral antigens are derived from 2-10 types of viruses.
  • the viral antigens are derived from 2-4 types of viruses.
  • the viral antigens are derived from 4-20 types of viruses.
  • the viral antigens are derived from 4-10 types of viruses.
  • the viral antigens are derived from 4-8 types of viruses.
  • the viral antigens are derived from 4-6 types of viruses.
  • viruses from which antigens can be derived include, but are not limited to, Epstein-Barr virus (EBV), Adenovirus (Adv), cytomegalovirus (CMV), cold viruses, flu viruses, hepatitis A, B, and C viruses, herpes simplex, HIV, influenza, Japanese encephalitis, measles, polio, rabies, respiratory syncytial, rubella, smallpox, varicella zoster, rotavirus, West Nile virus, Polyomavirus (e.g. BK Virus), zika virus, parvovirus (e.g. parvovirus B19), varicella-zoster virus (VZV), Herpes simplex virus (HSV), severe acute respiratory syndrome (SARS), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
  • EBV Epstein-Barr virus
  • Adv Adenovirus
  • CMV cytomegalovirus
  • HSV Herpes simplex virus
  • SARS severe acute respiratory syndrome
  • viruses and their respective antigens include, but are not limited to, BK Virus antigens include, but are not limited to, BKV LT; BKV (capsid VP1), BKV (capsid protein VP2), BKV (capsid protein VP2, isoporm VP3), BKV (small T antigen);
  • Adenovirus antigens include, but are not limited to, Adv-penton or Adv-hexon;
  • CMV antigens include, but are not limited to, envelope glycoprotein B, CMV IE-1 and CMV pp65, UL28, UL32,UL36, UL40, UL48,UL55,UL84, UL94, UL99 UL103, UL151, UL153, US 29, US 32;
  • EBV antigens include, but are not limited to, EBV LMP2, EBV BZLF1, EBV EBNA1, EBV P18, and EBV P23;
  • the antigen or antigens comprise viral peptides (or fragments thereof).
  • the viral peptides comprise 1-50, 1-40, 1-30, 1-25, 1-20, 1- 15, 1-10, 1-9, 1-8, 1-7, 1-6, 1-5, 1-4, 1-3, 1-2, 2-50, 2-40, 2-30, 2-20, 2-10, 2-8, 2-6, 2-4, 3-50, 3- 40, 3-30, 3-20, 3-10, 3-9, 3-7, 3-5, 3-4, 4-50, 4-40, 4-30, 4-20, 4-10, 4-8 or 4-6 viral peptides.
  • the viral peptides comprise 4-50 viral peptides (e.g. in a single formulation or in several formulations). According to a specific embodiment, the viral peptides comprise 4-40 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 4-30 viral peptides (e.g. in a single formulation or in several formulations). According to a specific embodiment, the viral peptides comprise 4-20 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 4-10 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 4-8 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 4-6 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45 or 50 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 4 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 5 viral peptides (e.g. in a single formulation or in several formulations). According to a specific embodiment, the viral peptides comprise 6 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 8 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 10 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 15 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 20 viral peptides (e.g. in a single formulation or in several formulations). According to a specific embodiment, the viral peptides comprise 30 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise 40 viral peptides (e.g. in a single formulation or in several formulations). According to a specific embodiment, the viral peptides comprise 50 viral peptides (e.g. in a single formulation or in several formulations).
  • the viral peptides comprise peptides from a single organism (i.e. from one virus type).
  • the viral peptides comprise peptides from two or more organism (i.e. a mixture from 2, 3, 4, 5 or more virus types).
  • the viral peptides comprise a BK vims peptide.
  • the viral peptides comprise at least one of an Epstein- Barr virus (EBV) peptide, a cytomegalovirus (CMV) peptide, a BK Virus peptide and an Adenovirus (Adv) peptide.
  • EBV Epstein- Barr virus
  • CMV cytomegalovirus
  • Adv Adenovirus
  • the viral peptides comprise an Epstein-Barr vims (EBV) peptide, a cytomegalovirus (CMV) peptide, a BK Vims peptide and an Adenovims (Adv) peptide.
  • EBV Epstein-Barr vims
  • CMV cytomegalovirus
  • Adv Adenovims
  • the viral peptides comprise at least one of EBV- LMP2, EBV-BZLF1, EBV-EBNA1, EBV-BRAF1, EBV-BMLF1, EBV-GP340/350 EBNA2, EBV-EBNA3a, EBV-EBNA3b, EBV-EBNA3c, CMV-pp65, CMV-IE-1, Adv-penton, Adv-hexon, BKV LT, BKV (capsid VP1), BKV (capsid protein VP2), BKV (capsid protein VP2, isoporm VP3), and BKV (small T antigen).
  • the viral peptides comprise at least one of AdV5 Hexon, hCMV pp65, EBV select (discussed below) and BKV LT.
  • Dedicated software can be used to analyze antigen sequences to identify immunogenic short peptides, i.e., peptides presentable in context of major histocompatibility complex (MHC) class I or MHC class II.
  • MHC major histocompatibility complex
  • the antigen or antigens comprise a mixture of pepmixes which are overlapping peptide libraries (e.g. 15mers overlapping by 11 amino acids) spanning the entire protein sequence of three viruses: CMV, EBV, and Adeno (such pepmixes can be commercially bought e.g. from JPT Technologies, Berlin, Germany).
  • pepmixes which are overlapping peptide libraries (e.g. 15mers overlapping by 11 amino acids) spanning the entire protein sequence of three viruses: CMV, EBV, and Adeno (such pepmixes can be commercially bought e.g. from JPT Technologies, Berlin, Germany).
  • the viral peptides comprise “EBV select” i.e. a commercial product from Miltenyi Biotec comprising 43 MHC class 1 and class 2 restricted peptides from 13 different proteins from EBV (e.g. MACS GMP PepTivator® EBV Select, e.g. catalog no. 170-076-143). Additionally or alternatively, the viral peptides comprise “collection EBV” i.e., a commercial product from JPT have comprising a pepmix which includes peptides from 14 different EBV antigens.
  • the viral peptides comprise PepMixTM BKV (capsid protein VP1), PepMixTM BKV (capsid protein VP2), PepMixTM BKV (capsid protein VP2, isoform VP3), PepMixTM BKV (large T antigen),
  • PepMixTM BKV small T antigen
  • the antigen or antigens comprise a mixture of seven pepmixes spanning EBV-LMP2, EBV-BZLF1, EBV-EBNA1, CMV-pp65, CMV-IE-1, Adv- penton and Adv-hexon at a concentration of e.g. 100 ng/peptide or 700 ng/mixture of the seven peptides.
  • the antigen or antigens comprise antigen or antigens of an infectious organism (e.g., bacterial, fungal organism) which typically affects immune comprised subjects, such as transplantation patients.
  • an infectious organism e.g., bacterial, fungal organism
  • the antigen is a bacterial antigen, such as but not limited to, an antigen of anthrax; gram-negative bacilli, chlamydia, diptheria, haemophilus influenza, Helicobacter pylori, malaria, Mycobacterium tuberculosis, pertussis toxin, pneumococcus, rickettsiae, staphylococcus, streptococcus and tetanus.
  • an antigen of anthrax gram-negative bacilli, chlamydia, diptheria, haemophilus influenza, Helicobacter pylori, malaria, Mycobacterium tuberculosis, pertussis toxin, pneumococcus, rickettsiae, staphylococcus, streptococcus and tetanus.
  • anthrax antigens include, but are not limited to, anthrax protective antigen; gram-negative bacilli antigens include, but are not limited to, lipopolysaccharides; haemophilus influenza antigens include, but are not limited to, capsular polysaccharides; diptheria antigens include, but are not limited to, diptheria toxin; Mycobacterium tuberculosis antigens include, but are not limited to, mycolic acid, heat shock protein 65 (HSP65), the 30 kDa major secreted protein and antigen 85A; pertussis toxin antigens include, but are not limited to, hemagglutinin, pertactin, FIM2, FIM3 and adenylate cyclase; pneumococcal antigens include, but are not limited to, pneumolysin and pneumococcal capsular polysaccharides; rickettsiae antigens include
  • the antigen is a superbug antigen (e.g. multi-drug resistant bacteria).
  • superbugs include, but are not limited to, Enterococcus faecium, Clostridium difficile, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacteriaceae (including Escherichia coli, Klebsiella pneumoniae, Enterobacter spp.).
  • the antigen is a fungal antigen.
  • fungi include, but are not limited to, Candida, coccidiodes, cryptococcus, histoplasma, leishmania, plasmodium, protozoa, parasites, schistosomae, tinea, toxoplasma, and trypanosoma cruzi.
  • coccidiodes antigens include, but are not limited to, spherule antigens; cryptococcal antigens include, but are not limited to, capsular polysaccharides; histoplasma antigens include, but are not limited to, heat shock protein 60 (HSP60); leishmania antigens include, but are not limited to, gp63 and lipophosphoglycan; plasmodium falciparum antigens include, but are not limited to, merozoite surface antigens, sporozoite surface antigens, circumsporozoite antigens, gametocyte/gamete surface antigens, protozoal and other parasitic antigens including the blood-stage antigen pf 155/RESA; schistosomae antigens include, but are not limited to, glutathione-S-transferase and paramyosin; tinea fungal antigens include, but are not limited to, trichophytin;
  • the antigen or antigens comprise antigens associated with a malignant disease (e.g. tumor antigens).
  • the antigen is an antigen (or part thereof, e.g. antigen epitope) expressed by tumor cells.
  • the antigen (or part thereof) is derived from a protein expressed in a hematopoietic tissue (e.g. hematopoietic malignancy such as leukemia antigen) or expressed in a solid tumor (e.g. melanoma, pancreatic cancer, liver cancer, gastrointestinal cancer, etc.).
  • tumor antigens include, but are not limited to, A33, BAGE, Bcl-2, B cell maturation antigen (BCMA), BCR-ABL, b-catenin, cancer testis antigens (CTA e.g. MAGE-1, MAGE-A2/A3 and NY-ESO-1), CA 125, CA 19-9, CA 50, CA 27.29 (BR 27.29), CA 15-3, CD5, CD19, CD20, CD21, CD22, CD33, CD37, CD45, CD123, CEA, c-Met, CS-1, cyclin Bl, DAGE, EBNA, EGFR, ELA2, ephrinB2, estrogen receptor, FAP, ferritin, folate-binding protein, GAGE, G250/CA IX, GD-2, GM2, gp75, gplOO (Pmel 17), HA-1, HA-2, HER-2/neu, HM1.24, HPV E6, HPV E7, hTERT, Ki-67
  • the antigen or antigens comprise a mixture of antigens (e.g. a mixture of antigens of one group of antigens as discussed, e.g. viral antigens; or a mixture of antigens from different groups of antigens, e.g. viral and bacterial antigens, viral and tumor antigens).
  • a mixture of antigens e.g. a mixture of antigens of one group of antigens as discussed, e.g. viral antigens; or a mixture of antigens from different groups of antigens, e.g. viral and bacterial antigens, viral and tumor antigens.
  • the antigen or antigens comprise a mixture of viral peptides and tumor peptides (e.g. in a single formulation or in several formulations). According to one embodiment, the antigen or antigens comprise a mixture of viral peptides and bacterial peptides (e.g. in a single formulation or in several formulations).
  • the antigen or antigens comprise a mixture of viral peptides and fungal peptides (e.g. in a single formulation or in several formulations).
  • loading of antigen presenting cells (e.g. mDCs) with an antigen or antigens can be carried out using any method known in the art.
  • the viral peptides are co-cultured with the antigen presenting cells (e.g. mDCs) for 30 minutes to 3 hours (e.g. 1 hour) at 37 °C at 5 % CO2.
  • antigen presenting cells e.g. mDCs
  • peptivators e.g. AdV5 Hexon, HCMV pp65, EBV select and BKV LT
  • the viral peptide loaded antigen presenting cells e.g. mDCs
  • the viral peptide loaded antigen presenting cells are washed and centrifuged with e.g. ACD-A with 0.5 % of 25 % HAS and DPBS Buffer, and are resuspended in cell growth medium (e.g. T cell growth medium).
  • the antigen or antigens (e.g. viral peptide) loaded antigen presenting cells are irradiated via X-Ray source.
  • the loaded antigen presenting cells e.g. mDCs
  • the DCs are irradiated with about 10-40 Gy (e.g. 25-30 Gy e.g. 30 Gy).
  • the loaded antigen presenting cells e.g. viral peptide loaded mDCs are washed, centrifuged and resuspended in cell growth medium (e.g. T cell growth medium).
  • the antigen-loaded antigen presenting cells e.g. mDCs
  • Tcm cells are then ready to use for generation of Tcm cells from the population of cells comprising memory CD8 T cells according to some embodiments of the invention.
  • the antigen presenting cells comprise dendritic cells
  • the antigen presenting cells comprise mature dendritic cells (mDC).
  • the antigen presenting cells comprise mature dendritic cells (mDC).
  • the antigen presenting cells comprise irradiated dendritic cells.
  • the antigen presenting cells are of the same donor subject as the population of cells (as discussed below).
  • antigen presenting cells may express all of the antigens on a single cell or may express only part of the antigens on a single cell. Moreover, different antigen presenting cells (e.g. in the same preparation) may express different antigens. Accordingly, the antigen presenting cells (e.g. mDC) comprise a heterogeneous cell mixture.
  • the antigen or antigens can be presented by genetically modified antigen presenting cells or artificial antigen presenting cells exhibiting MHC antigens (also referred to as human leukocyte antigen (HLA)) recognizable by the memory CD8 T cells (e.g. cell line transfected with the antigen or antigens).
  • MHC antigens also referred to as human leukocyte antigen (HLA)
  • HLA human leukocyte antigen
  • antigen or antigens e.g. viral peptides
  • an artificial vehicle e.g. liposome
  • antigen presenting cells as discussed above
  • cell lines, artificial vehicles (such as a liposome) or artificial antigen presenting cells e.g. leukemic or fibroblast cell line transfected with the antigen or antigens
  • artificial antigen presenting cells e.g. leukemic or fibroblast cell line transfected with the antigen or antigens
  • Such short peptides, protein extracts or purified proteins may be viral-, bacterial-, fungal-, or tumor-antigen derived peptides or peptides representing any other antigen.
  • the method of some embodiments of the invention is affected by providing a population of cells comprising T cells, wherein the T cells in the population of cells comprise at least 40 % memory T cells expressing a CD45RA-CD8 + phenotype and depleted of CD4 + , CD56 + and CD45RA + expressing cells.
  • the term "population of cells comprising T cells” refers to a heterogeneous mixture of PBMCs comprising T cells, B cells and myeloid cells.
  • the population of cells comprising T cells typically comprises T cells having numerous signatures, functions and capable of binding various antigens (e.g. cytotoxic T cells, memory T cells, effector T cells etc.).
  • the population of cells comprising T cells does not comprise erythrocytes and granulocytes.
  • memory T cells refers to a subset of T lymphocytes which have previously encountered and responded to an antigen, also referred to as antigen experienced T cells.
  • the memory T cells comprise at least about 20 %, at least about 30 %, at least about 40 %, at least about 50 %, at least about 60 %, at least about 70 %, at least about 80 %, at least about 90 %, at least about 95 %, at least about 99 %, or even 100 % of the T cells in the population of cells.
  • the level of memory T cells comprises less than 20 % of the total number of cells in a population of cells comprising T cells.
  • the memory T cells comprise T cells expressing a CD8 marker (i.e. CD8 + T cells).
  • the memory T cells comprise a CD8 + CD45RO + phenotype.
  • the memory T cells comprise a CD8 + CD45RA- phenotype.
  • the memory T cells comprise a CD8 + CD45RO + CD45RA- phenotype.
  • the memory T cells are devoid of CD45RA + cells.
  • the memory T cells are devoid of CD4 + and/or CD56 + cells.
  • Selection of memory CD8 + T cells may be affected by selection of cells co-expressing CD8 + and CD45RA- and/or cells co-expressing CD8 + and CD45RO + and may be carried out using any method known in the art, such as by affinity based purification (e.g. such as by the use of MACS beads, FACS sorter and/or capture ELISA labeling).
  • affinity based purification e.g. such as by the use of MACS beads, FACS sorter and/or capture ELISA labeling.
  • Selection of memory CD8 + T cells may be further affected by selection of effector T cells and central memory T cells, the latter expressing e.g. CD62L, CCR7, CD27 and/or CD28.
  • PBMCs may be depleted of naive cells, e.g. CD45RA + cells, of CD4 + cells (e.g. T helper cells), of CD56 + cells (e.g. NK cells) or any other cells not comprising a memory T cell phenotype.
  • naive cells e.g. CD45RA + cells, of CD4 + cells (e.g. T helper cells), of CD56 + cells (e.g. NK cells) or any other cells not comprising a memory T cell phenotype.
  • CD4 + and/or CD56+ cells may be carried out using any method known in the art, such as by affinity based purification (e.g. such as by the use of MACS beads, FACS sorter and/or capture ELISA labeling).
  • memory T cells are obtained from peripheral blood mononuclear cells (PBMCs).
  • PBMCs peripheral blood mononuclear cells
  • memory T cells are obtained by a method comprising treating a second population of PBMCs of the same donor subject as the first population of PBMCs with one or more agents capable of depleting CD4 + , CD56 + and CD45RA + expressing cells so as to obtain a population of cells which include at least 40 % memory T cells expressing a CD45RA- CD8 + phenotype.
  • the population of cells further comprises B cells and myeloid cells.
  • the CD14- cells collected from the first population of cells are combined with the second population of cells prior to enrichment of memory T cells.
  • the CD14- cells obtained from the first population of cells and/or the PBMCs obtained from the second population of cells are centrifuged and resuspended at a concentration of e.g. 10-50 x 10 6 cells/ml, e.g. 30 x 10 6 cells/ml, in cell growth media e.g. T Cell Growth Media (e.g. Click’s Media with advanced RPMI 1640 supplemented with 1:100 Glutamaxe and 5% Human AB Serum) along with IL-7 (30 IU/mL)).
  • the cell growth media is supplemented with IL-7 (e.g. at a concentration of e.g.
  • the cell suspension is then seeded (e.g. in tissue culture flasks) and incubated for 12-36 hours, e.g. for 16-24 hours, e.g. for 24 hours, in at 37 °C, 5 % CO 2 .
  • the second population of PBMCs are centrifuged and resuspended in buffer (e.g. in CliniMACS ® /0.5 % HSA Buffer) to a minimum of 1:2 ratio.
  • buffer e.g. in CliniMACS ® /0.5 % HSA Buffer
  • the second population of PBMCs are platelet washed (e.g. thrombowash), centrifuged and resuspended in in buffer (e.g. CliniMACS ® /0.5 % HSA buffer).
  • the post-platelet depleted cell preparation of one embodiment is incubated with IVIg for 5-30 minutes e.g. 10-15 minutes. After the initial incubation the CD4 + , CD56 + and CD45RA + binding agents are added to the cell preparation and incubated for e.g. 10-60 minutes, e.g. 30 minutes, on an orbital rotator.
  • the CD4 + , CD56 + and/or CD45RA + binding agent is an antibody.
  • the CD4 + , CD56 + and/or CD45RA + binding agent is a monoclonal antibody.
  • the CD4 + , CD56 + and/or CD45RA + monoclonal antibody is conjugated to magnetic particles.
  • the CD4 + , CD56 + and/or CD45RA + monoclonal antibody is conjugated to super-paramagnetic particles.
  • the cells are washed by centrifugation and the cell pellet resuspended in buffer (e.g. CliniMACS ® /0.5 % HSA buffer) to remove excess reagent.
  • buffer e.g. CliniMACS ® /0.5 % HSA buffer
  • the CD4 + /CD56 + /CD45RA + labeled cells are selected by magnetic separation techniques (as discussed in detail hereinabove).
  • the CD4 + /CD56 + /CD45RA + labeled cells are processed on CliniMACS ® column.
  • the CD4 + /CD56 + /CD45RA + magnetically labeled cells i.e. CD4 + /CD56 + /CD45RA + expressing cells
  • the separation column i.e. negative selection
  • the CD4-/CD56-/CD45RA- cells are collected.
  • the collected cells are washed and resuspended in T cell Growth Medium.
  • samples from each fraction are removed for cell count, viability and/or immunopheno typing .
  • the collected CD4-/CD56-/CD45RA- cell fraction is adjusted at 0.01-10 x 10 6 cells/ml, e.g. 2 x 10 6 cells/ml, in T Cell Growth Media supplemented with cytokines and growth factors. Determination of cytokines and growth factors to be used is within the skill of a person of skill in the art.
  • the T Cell Growth Media is supplemented with IL-7 (e.g. at a concentration of e.g. 1-100 IU/mL, e.g. 30 IU/mL).
  • the cell suspension is then seeded (e.g. in G-Rex ® 100) and incubated for 12-36 hours, e.g. for 16-24 hours, e.g. for 24 hours, in at 37 °C, 5 % CO 2 .
  • the memory T cells are contacted with the antigenic peptides, e.g. viral peptides.
  • antigen e.g. viral antigen
  • the non-GVHD inducing cells comprising a Tcm phenotype of the present invention are generated by contacting a population of cells comprising memory T cells with antigen presenting cells loaded with the antigenic peptides e.g. viral peptides (such as described above) in a culture supplemented with IL-21 (e.g. in an otherwise cytokine-free culture i.e., without the addition of any additional cytokines).
  • antigenic peptides e.g. viral peptides (such as described above)
  • IL-21 e.g. in an otherwise cytokine-free culture i.e., without the addition of any additional cytokines.
  • This step is typically carried out for about 12-24 hours, about 12-36 hours, about 12-72 hours, 12-96 hours, 12-120 hours, about 24-36 hours, about 24-48 hours, about 24-72 hours, about 36-48 hours, about 36-72 hours, about 48-72 hours, about 48-96 hours, about 48-120 hours, 0.5-1 days, 0.5-2 days, 0.5-3 days, 0.5-5 days, 1-2 days, 1-3 days, 1-5 days, 1- 7 days, 1-10 days, 2-3 days, 2-4 days, 2-5 days, 2-6 days, 2-8 days, 3-4 days, 3-5 days, 3-7 days, 4- 5 days, 4-8 days, 5-7 days, 6-8 days or 8-10 days and allows enrichment of antigen (e.g. viral antigen) reactive cells.
  • antigen e.g. viral antigen
  • contacting a population of PBMC depleted of CD4+, CD56+ and CD45RA+ cells and comprising memory CD8 + T cells e.g. a population of cells comprising T cells, wherein the T cells in the population of cells comprise at least 40 % memory T -ells
  • an antigen or antigens such as described above
  • a culture supplemented with IL-21 otherwise cytokine-free culture
  • Contacting a population of cells comprising memory CD8 + T cells with an antigen or antigens (such as described above) in a culture supplemented with IL-21 is typically carried out in the presence of about 0.001-3000 IU/ml, 0.01-3000 IU/ml, 0.1-3000 IU/ml, 1-3000 IU/ml, 10-3000 IU/ml, 100-3000 IU/ml, 1000-3000 IU/ml, 0.001-1000 IU/ml, 0.01-1000 IU/ml, 0.1-1000 IU/ml, 1- 1000 IU/ml, 10-1000 IU/ml, 100-1000 IU/ml, 250-1000 IU/ml, 500-1000 IU/ml, 750-1000 IU/ml, 10-500 IU/ml, 50-500 IU/ml, 100-500 IU/ml, 250-500 IU/ml, 100-250 IU/ml, 0.1-100 IU/ml, 1-100
  • contacting a population of cells comprising memory CD8 + T cells with an antigen or antigens is affected in a cytokine-free culture (e.g. supplemented with only IL-21), such a culture condition enables survival and enrichment of only those cells which undergo stimulation and activation by the antigen or antigens (i.e. of antigen reactive cells, e.g. viral reactive memory T cells) as these cells secrete cytokines (e.g. IL-2) which enable their survival (all the rest of the cells die under these culture conditions).
  • cytokine-free culture e.g. supplemented with only IL-21
  • the ratio of the population of cells comprising memory CD8 + T cells (i.e. CD4-CD56-CD45RA- cells) to antigenic (e.g. viral) peptide loaded antigen presenting cells (e.g. mDCs) is typically about 2:1 to about 10:1, such as about 2:1, about 3:1, about 4:1, about 5:1, about 6:1, about 8:1 or about 10:1.
  • the ratio of the population of cells comprising memory CD8 + T cells (i.e. CD4-CD56-CD45RA- cells) to antigenic (e.g. viral) peptide loaded antigen presenting cells (e.g. mDCs) is about 2:1 to 8:1, e.g. about 5:1.
  • the population of cells comprising memory CD8 + T cells are seeded (e.g. in G-Rex ® 100) at a concentration of 0.01-10 x 10 6 cells/ml, e.g. 1 x 10 6 cells/ml, together with the viral peptide loaded antigen presenting cells (e.g. mDCs) at a ratio of about 2:1 to about 8:1, e.g. about 5:1 (memory CD8 + T cells: antigen presenting cells (e.g. mDC)) in T Cell Growth Media along with IL-21 (e.g. at a concentration of 50-500 IU/ml, e.g. 100 IU/ml) for 1-5 days (e.g. 3 days) in 37 °C, 5 % CO2.
  • G-Rex ® 100 the population of cells comprising memory CD8 + T cells
  • the viral peptide loaded antigen presenting cells e.g. mDCs
  • IL-21 e.g. at a concentration of 50
  • the resultant population of cells comprising memory CD8 + T cells are cultured in the presence of IL-21, IL-15 and/or IL-7 so as to allow proliferation of cells comprising the Tcm phenotype.
  • This step is typically carried out for about 12-24 hours, about 12-36 hours, about 12-72 hours, about 12-96 hours, about 12-120 hours, about 12-240 hours, 24-36 hours, 24-48 hours, about 24-72 hours, 24-96 hours, 24-120 hours, 24-240 hours, about 48-72 hours, about 48-120 hours, about 48-240 hours, about 96-240 hours, about 120-144 hours, about 120-240 hours, about 144-240 hours, 0.5-1 days, 0.5-2 days, 0.5-3 days, 0.5-5 days, 0.5-10 days, 1- 2 days, 1-3 days, 1-4 days, 1-6 days, 1-8 days, 1-9 days, 1-10 days, 2-3 days, 2-4 days, 2-5 days, 2- 6 days, 2-8 days, 2-9 days, 2-10 days, 4-5 days, 4-6 days, 4-8 days, 4-9 days, 4-10 days, 5-6 days, 5-7 days, 5-8 days, 5-9 days, 5-10 days, 5-15 days, 6-7 days, 6-8 days, 6-9 days, 6-10 days, 6-12 days, 7-8 days,
  • the resultant population of cells comprising memory CD8 + T cells are cultured in the presence of IL-21, IL-15 and IL-7 for about 6-12 days (e.g. 9 days).
  • This step is typically carried out in the presence of IL-21 at a concentration of about 0.001- 3000 IU/ml, 0.01-3000 IU/ml, 0.1-3000 IU/ml, 1-3000 IU/ml, 10-3000 IU/ml, 100-3000 IU/ml, 1000-3000 IU/ml, 0.001-1000 IU/ml, 0.01-1000 IU/ml, 0.1-1000 IU/ml, 1-1000 IU/ml, 10-1000 IU/ml, 100-1000 IU/ml, 250-1000 IU/ml, 500-1000 IU/ml, 750-1000 IU/ml, 10-500 IU/ml, 50-500 IU/ml, 100-500 IU/ml, 250-500 IU/ml, 100-250 IU/ml, 0.1-100 IU/ml, 1-100 IU/ml, 10-100 IU/ml, 30-100 IU/ml, 50-100 IU/
  • This step is further carried out in the presence of IL-15 at a concentration of about 0.001- 3000 IU/ml, 0.01-3000 IU/ml, 0.1-3000 IU/ml, 1-3000 IU/ml, 10-3000 IU/ml, 100-3000 IU/ml, 125-3000 IU/ml, 1000-3000 IU/ml, 0.001-1000 IU/ml, 0.01-1000 IU/ml, 0.1-1000 IU/ml, 1-1000 IU/ml, 10-1000 IU/ml, 100-1000 IU/ml, 125-1000 IU/ml, 250-1000 IU/ml, 500-1000 IU/ml, 750- 1000 IU/ml, 10-500 IU/ml, 50-500 IU/ml, 100-500 IU/ml, 125-500 IU/ml, 250-500 IU/ml, 250-500 IU/ml, 125-250 IU/ml,
  • This step is further carried out in the presence of IL-7 at a concentration of about 0.001- 3000 IU/ml, 0.01-3000 IU/ml, 0.1-3000 IU/ml, 1-3000 IU/ml, 10-3000 IU/ml, 30-3000 IU/ml, 100- 3000 IU/ml, 1000-3000 IU/ml, 0.001-1000 IU/ml, 0.01-1000 IU/ml, 0.1-1000 IU/ml, 1-1000 IU/ml, 10-1000 IU/ml, 30-1000 IU/ml, 100-1000 IU/ml, 250-1000 IU/ml, 500-1000 IU/ml, 750- 1000 IU/ml, 10-500 IU/ml, 30-500 IU/ml, 50-500 IU/ml, 100-500 IU/ml, 250-500 IU/ml, 100-250 IU/ml, 0.1-100 IU/ml, 1-100 IU
  • the cell culture comprising the resultant population of cells comprising memory CD8 + T cells is supplemented with IL-7 (e.g. at a concentration of e.g. 1-100 IU/ml, e.g. 30 IU/mL), IL-15 (e.g. at a concentration of e.g. 50-500 IU/ml, e.g. 125 IU/mL) and IL-21 (e.g. at a concentration of 50-500 IU/ml, e.g. 100 IU/mL) at 50 % of the culture volume, and cultured for about 6-12 days (e.g. 9 days) while supplementing IL-7, IL-15, IL-21 every about 48-96 hours, e.g. 48 hours, e.g. 72 hours.
  • IL-7 e.g. at a concentration of e.g. 1-100 IU/ml, e.g. 30 IU/mL
  • IL-15 e.g. at a concentration of
  • the total length of culturing time for generating the Tcm cells is about 9, 10, 11, 12, 13, 14, 15, 17, 19 or 21 days (e.g. 12 days).
  • the cell culture comprising the resultant population of cells comprising memory CD8 + T cells is monitored for glucose levels.
  • the glucose is at a level comprising 10-500 mg/dl, e.g. 50- 170 mg/dl.
  • cytokines IL-7, IL-15, IL-21 are added to the culture (as detailed above).
  • fresh T cell Growth medium e.g. 25 % volume, e.g. 100 ml (e.g. 25 % of the G-Rex ® 100 volume of 400 ml) plus cytokines IL-7, IL-15, IL-21 are added to the culture.
  • fresh T cell Growth medium e.g. 50 % volume, e.g. 200 ml (e.g. 50 % of the G-Rex ® 100 volume of 400 ml) plus cytokines IL-7, IL-15, IL-21 are added to the culture.
  • culturing further comprises adding glucose to a concentration of at least about 20 mg/dl, at least about 30 mg/dl, at least about 40 mg/dl, at least about 50 mg/dl, at least about 60 mg/dl, at least about 70 mg/dl, at least about 80 mg/dl, at least about 90 mg/dl, at least about 100 mg/dl
  • culturing further comprises adding glucose to a concentration of at least about 50 mg/dl.
  • the cell culture comprising the resultant population of cells comprising memory CD8 + T cells is monitored for pH levels.
  • the pH is at the physiologic range (e.g. pH 7.2-7.6). In case the pH level is not at a physiological range, the pH level may be adjusted using any method known in the art.
  • a method of generating an isolated population of non-graft versus host disease (GVHD) inducing cells comprising a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation, the method comprising:
  • PBMCs peripheral blood mononuclear cells
  • step (d) contacting the population of cells comprising the memory T cells with the dendritic cells loaded with the viral peptides of step (b) in the presence of IL-21 so as to allow enrichment of viral reactive memory T cells;
  • step (e) culturing the cells resulting from step (d) in the presence of IL-21, IL-15 and/or IL-7 so as to allow proliferation of cells comprising the Tcm phenotype, thereby generating the isolated population of non-GVHD inducing cells comprising the Tcm phenotype.
  • culturing the resultant population of cells comprising memory CD8 + T cells i.e. after culture with IL-21
  • IL-21 IL-15
  • IL-7 is typically affected in an antigen free environment (i.e. without the addition of an antigen or antigens, e.g. viral peptides).
  • an antigen or antigens e.g. viral peptides
  • residual antigen or antigens can be present in the cell culture after culture with IL-21 (i.e.
  • an antigen free environment relates to a cell culture without the addition of supplementary antigen presenting cells presenting antigen or antigens (e.g. viral peptides).
  • supplementary antigen presenting cells presenting antigen or antigens (e.g. viral peptides).
  • the antigen/s e.g. tumor antigen, viral antigen
  • the antigen/s is administered to the donor subject prior to obtaining memory CD8 + T cells therefrom (e.g. prior to providing the population of T cells comprising at least 40 % memory CD8 + T cells).
  • Any method of immunizing a cell donor against an antigen in order to elicit an immunogenic response e.g. generation of memory CD8 + T cells
  • the antigen may be administered as is or as part of a composition comprising an adjuvant (e.g. Complete Freund's adjuvant (CFA) or Incomplete Freund's adjuvant (IFA)).
  • CFA Complete Freund's adjuvant
  • IFA Incomplete Freund's adjuvant
  • the antigen is administered to a donor subject once.
  • the donor subject receives at least one additional (e.g. boost) administration of the antigen (e.g. 2, 3, 4 or more administrations).
  • additional administration may be affected 1, 3, 5, 7, 10, 12, 14, 21, 30 days or more following the first administration of the antigen.
  • the population of cells comprising memory CD8 + T cells may be further contacted with the same antigen or antigens (e.g. the same antigen as administered to the cell donor), as described hereinabove.
  • cell samples and culture medium samples can be obtained at any stage during the process of generating the isolate population of non-GVHD inducing cells comprising the Tcm phenotype. These can be used for evaluating cell count, cell viability, sterility, immunophenotyping, glucose and pH levels, etc. Any method known in the art can be used to implement such procedures.
  • an isolated population of non-GVHD inducing cells comprising cells having a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation, generated according to the method of some embodiments of the invention.
  • Tcm central memory T-lymphocyte
  • the cells may be used as fresh cells (e.g. within about 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 days, e.g. within about 3 days).
  • the cells may be cryopreserved until needed (e.g. for 1 week, 2 weeks, 1 month, 2 months, 4 months, 6 months, a year or more).
  • the non-GVHD inducing cells comprising the Tcm phenotype of the invention are not naturally occurring and are not a product of nature. These cells are typically produced by ex-vivo manipulation (i.e. exposure to an antigen or antigens in the presence of specific cytokines).
  • the above describe protocols are typically used for non-syngeneic applications and therefore the population of cells comprising memory T cells or PBMC used are typically allogeneic with respect to a recipient subject (e.g. from an allogeneic donor).
  • the memory T cells or PBMC used may be of a xenogeneic origin as discussed below.
  • the population of cells comprising memory T cells or PBMC used may be autologous with respect to a recipient subject (e.g. from the subject).
  • the population of cells comprising memory CD8 + T cells or PBMC may be syngeneic or non-syngeneic with respect to a subject.
  • syngeneic cells refer to cells which are essentially genetically identical with the subject or essentially all lymphocytes of the subject.
  • Examples of syngeneic cells include cells derived from the subject (also referred to in the art as an “autologous”), from a clone of the subject, or from an identical twin of the subject.
  • non-syngeneic cells refer to cells which are not essentially genetically identical with the subject or essentially all lymphocytes of the subject, such as allogeneic cells or xenogeneic cells.
  • allogeneic refers to cells which are derived from a donor subject who is of the same species as the recipient subject, but which is substantially non-clonal with the recipient subject. Typically, outbred, non-zygotic twin mammals of the same species are allogeneic with each other. It will be appreciated that an allogeneic cell may be HLA identical, partially HLA identical or HLA non-identical (i.e. displaying one or more disparate HLA determinant) with respect to the recipient subject.
  • the donor is a human being.
  • xenogeneic refers to a cell which substantially expresses antigens of a different species relative to the species of a substantial proportion of the lymphocytes of the subject. Typically, outbred mammals of different species are xenogeneic with each other.
  • xenogeneic cells are derived from a variety of species.
  • the cells may be derived from any mammal.
  • Suitable species origins for the cells comprise the major domesticated or livestock animals and primates.
  • Such animals include, but are not limited to, porcines (e.g.
  • pig bovines (e.g., cow), equines (e.g., horse), ovines (e.g., goat, sheep), felines (e.g., Felis domestica ), canines (e.g., Canis domestica ), rodents (e.g., mouse, rat, rabbit, guinea pig, gerbil, hamster), and primates (e.g., chimpanzee, rhesus monkey, macaque monkey, marmoset).
  • Cells of xenogeneic origin e.g. porcine origin
  • human-derived cells or tissues are preferably obtained from substantially pathogen-free sources.
  • the source of the population of cells comprising memory CD8 + T cells or PBMCs will be determined with respect to the intended use of the cells (see further details hereinbelow) and is well within the capability of one skilled in the art, especially in light of the detailed disclosure provided herein.
  • veto cells are especially beneficial in situations in which there is a need to eliminate graft rejection, overcome graft versus host disease (GVHD) and/or to induce donor specific tolerance, such as in transplantation of allogeneic or xenogeneic cells or tissues.
  • GVHD graft versus host disease
  • the use of veto cells can also be beneficial for prolongation of “off-the-shelf’ allogenic genetically modified T cells such as CAR-T cells or TCR transgenic T cells (TCR-T).
  • the veto cells of some embodiments of the invention may be used as an adjuvant therapy to facilitate engraftment of genetically modified cells transduced to express a cell surface receptor comprising a T cell receptor signaling module (i.e. an intracellular portion of the receptor responsible for activation of at least one of the normal effector functions of the T cell in which the receptor has been placed in), e.g. a transgenic T cell receptor (tg-TCR) or a chimeric antigen receptor (CAR), as discussed in detail herein above.
  • a T cell receptor signaling module i.e. an intracellular portion of the receptor responsible for activation of at least one of the normal effector functions of the T cell in which the receptor has been placed in
  • tg-TCR transgenic T cell receptor
  • CAR chimeric antigen receptor
  • the veto cells of some embodiments of the invention can be used in conjunction with any CAR-T or TCR-T cells (e.g. CAR-T or TCR-T cells generated from cells of the veto cell donor), such as tumor specific CAR-T or TCR-T cells targeting a variety of tumor antigens for the treatment of hematopoietic or solid cancers.
  • CAR-T or TCR-T cells e.g. CAR-T or TCR-T cells generated from cells of the veto cell donor
  • tumor specific CAR-T or TCR-T cells targeting a variety of tumor antigens for the treatment of hematopoietic or solid cancers.
  • cancers and their antigens that can be targeted include, but are not limited to, follicular lymphoma (CD20 or GD2), neuroblastoma (CD171), non-Hodgkin lymphoma (CD20), lymphoma (CD19), glioblastoma (IL13Ra2), chronic lymphocytic leukemia or CLL, Multiple Myeloma (BCMA, CD138, CS1) and acute lymphocytic leukemia or ALL (both CD 19).
  • follicular lymphoma CD20 or GD2
  • neuroblastoma CD171
  • CD20 non-Hodgkin lymphoma
  • CD19 lymphoma
  • IL13Ra2 glioblastoma
  • CLL chronic lymphocytic leukemia or CLL
  • BCMA Multiple Myeloma
  • ALL acute lymphocytic leukemia
  • the veto cells and the CAR-T/TCR-T cells can be used concomitantly or subsequent to each other (e.g. on the same day or within e.g. about 1, 2, 3, 4, 5, 6, 7 days of each other).
  • the veto cells of the invention are further endowed with anti-disease activity (e.g. anti-viral activity or anti-tumor activity e.g. GVL) and are therefore beneficial in situations in which a subject, e.g. transplanted subject, has a disease or condition (e.g. malignant, viral, bacterial, fungal, autoimmune or allergic disease or condition), pre- or post- transplantation (e.g. before immune reconstitution is established).
  • anti-disease activity e.g. anti-viral activity or anti-tumor activity e.g. GVL
  • GVL anti-disease activity
  • the veto cells of the invention are beneficial for preventing viral infection.
  • the veto cells of the invention are beneficial for killing cancer cells including residual cancer cells (e.g. leukemic cells). According to one embodiment, the veto cells of the invention are beneficial for preventing disease relapse (e.g. leukemia relapse).
  • a method of treating a disease in a subject in need thereof comprising administering to the subject a therapeutically effective amount of the isolated population of non-GVHD inducing cells of some embodiments of the invention (i.e. Tcm cells), thereby treating the disease in the subject.
  • a therapeutically effective amount of the isolated population of non-GVHD inducing cells of some embodiments of the invention i.e. Tcm cells
  • non-GVHD inducing cells comprising a Tcm phenotype of some embodiments of the invention for the manufacture of a medicament identified for treating a disease in a subject in need thereof.
  • treating includes abrogating, substantially inhibiting, slowing or reversing the progression of a condition, substantially ameliorating clinical or aesthetical symptoms of a condition or substantially preventing the appearance of clinical or aesthetical symptoms of a condition.
  • the term "subject” or “subject in need thereof’ refers to a mammal, preferably a human being, male or female at any age that is in need of a cell or tissue transplantation or suffers from a disease which may be treated with the non-GVHD inducing Tcm cells.
  • the subject is in need of cell or tissue transplantation (also referred to herein as recipient) due to a disorder or a pathological or undesired condition, state, or syndrome, or a physical, morphological or physiological abnormality which is amenable to treatment via cell or tissue transplantation. Examples of such disorders are provided further below.
  • the method of the present invention may be applied to treat any disease such as, but not limited to, a malignant disease, a disease associated with transplantation of a graft (e.g. graft rejection, graft versus host disease), an infectious disease (e.g. viral disease, fungal disease or a bacterial disease), an inflammatory disease, an autoimmune disease and/or an allergic disease or condition.
  • a malignant disease e.g. graft rejection, graft versus host disease
  • an infectious disease e.g. viral disease, fungal disease or a bacterial disease
  • the subject has a malignant disease.
  • Malignant diseases also termed cancers
  • Malignant diseases which can be treated by the method of some embodiments of the invention can be any solid or non- solid tumor and/or tumor metastasis and/or disease relapse.
  • cancer examples include, but are not limited to, carcinoma, lymphoma, blastoma, sarcoma, and leukemia. More particular examples of such cancers include squamous cell cancer, soft-tissue sarcoma, Kaposi's sarcoma, melanoma, lung cancer (including small-cell lung cancer, non- small-cell lung cancer, adenocarcinoma of the lung, and squamous carcinoma of the lung), cancer of the peritoneum, hepatocellular cancer, gastric or stomach cancer (including gastrointestinal cancer), pancreatic cancer, glioblastoma, cervical cancer, ovarian cancer, liver cancer, bladder cancer, hepatoma, breast cancer, colon cancer, colorectal cancer, rectal cancer, endometrial or uterine carcinoma, carcinoid carcinoma, salivary gland carcinoma, kidney or renal cancer, liver cancer, prostate cancer, vulval cancer, thyroid cancer, hepatic carcinoma, mesothelioma, multiple myeloma,
  • the malignant disease is a leukemia, a lymphoma, a myeloma, a melanoma, a sarcoma, a neuroblastoma, a colon cancer, a colorectal cancer, a breast cancer, an ovarian cancer, an esophageal cancer, a synovial cell cancer, a hepatic cancer and a pancreatic cancer .
  • the malignant disease is a hematological malignancy.
  • hematological malignancies include, but are not limited to, leukemia [e.g., acute lymphatic, acute lymphoblastic, acute lymphoblastic pre-B cell, acute lymphoblastic T cell leukemia, acute - megakaryoblastic, monocytic, acute myelogenous, acute myeloid, acute myeloid with eosinophilia, B cell, basophilic, chronic myeloid, chronic, B cell, eosinophilic, Friend, granulocytic or myelocytic, hairy cell, lymphocytic, megakaryoblastic, monocytic, monocytic- macrophage, myeloblastic, myeloid, myelomonocytic, plasma cell, pre-B cell, promyelocytic, subacute, T cell, lymphoid neoplasm, predisposition to myeloid malignancy, acute
  • the hematological malignancy is a follicular lymphoma (FL), mantle cell lymphoma (MCL), chronic lymphocytic leukemia (CLL), multiple myeloma (MM), Hodgkin’s lymphoma (HL), non-hodgkin’s lymphoma (NHL), chronic myeloid leukemia (CML), myeloproliferative syndromes (MPD), acute myeloid leukemia (AML) or acute lymphoid leukemia (ALL).
  • FL follicular lymphoma
  • MCL mantle cell lymphoma
  • CLL chronic lymphocytic leukemia
  • MM multiple myeloma
  • NHL Hodgkin’s lymphoma
  • NHL non-hodgkin’s lymphoma
  • CML chronic myeloid leukemia
  • MPD myeloproliferative syndromes
  • AML acute myeloid leukemia
  • the subject has a non-malignant disease.
  • the non-malignant disease is an organ dysfunction or failure, a hematologic disease, a graft related disease, an infectious disease, an inflammatory disease, an autoimmune disease, an allergic disease, a genetic disease or disorder, a metabolic disorder, a trauma and an injury.
  • the subject of the present invention may suffer from any of a cardiovascular disease, a rheumatoid disease, a glandular disease, a gastrointestinal disease, a cutaneous disease, a hepatic disease, a neurological disease, a muscular disease, a nephric disease, a connective tissue disease, a systemic disease and/or a disease related to reproduction, treatable by cell or tissue transplantation.
  • non-malignant diseases include, but are not limited to, severe combined immunodeficiency syndromes (SCID), sickle cell disease (e.g. sickle cell anemia), congenital neutropenia, thrombocytopenia, aplastic anemia (e.g.
  • SCID severe combined immunodeficiency syndromes
  • sickle cell disease e.g. sickle cell anemia
  • congenital neutropenia e.g. thrombocytopenia
  • aplastic anemia e.g.
  • aplastic anemia myelodysplastic syndrome, monosomy 7, osteopetrosis, Gaucher's disease, Hurler's disease, metachromatic leukodystrophy, adrenal leukodystrophy, thalassemia, congenital or genetically-determined hematopoietic abnormality, adenosine deaminase (ADA), lupus, autoimmune hepatitis, celiac disease, type I diabetes mellitus, Grave's disease, Guillain-Barr syndrome, Myasthenia gravis, Rheumatoid arthritis, scleroderma and psoriasis.
  • ADA adenosine deaminase
  • the non-malignant disease may be treated by transplantation of hematopoietic progenitor cells assisted by the addition of veto cells.
  • the non-malignant disease may be treated by transplantation of a cell or tissue graft (as discussed below), assisted by transplantation of hematopoietic progenitor cells and veto cells (e.g. inducing donor chimerism).
  • infectious diseases include, but are not limited to, chronic infectious diseases, subacute infectious diseases, acute infectious diseases, viral diseases, bacterial diseases, protozoan diseases, parasitic diseases, fungal diseases, mycoplasma diseases and prion diseases .
  • viral pathogens causing infectious diseases treatable according to the teachings of the present invention include, but are not limited to, retroviruses, circoviruses, parvoviruses, papovaviruses, adenoviruses, herpesviruses, iridoviruses, poxviruses, hepadnaviruses, picornaviruses, caliciviruses, togaviruses, flaviviruses, reoviruses, orthomyxoviruses, paramyxoviruses, rhabdoviruses, bunyaviruses, coronaviruses, arenaviruses, and filoviruses.
  • viral infections include, but are not limited to, those caused by human immunodeficiency virus (HlV)-induced acquired immunodeficiency syndrome (AIDS), influenza, rhinoviral infection, viral meningitis, Epstein-Barr virus (EBV) infection, hepatitis A, B or C virus infection, measles, papilloma virus infection/warts, cytomegalovirus (CMV) infection, Herpes simplex virus infection, yellow fever, Ebola virus infection, rabies, Adenovirus (Adv), cold viruses, flu viruses, Japanese encephalitis, polio, respiratory syncytial, rubella, smallpox, varicella zoster, rotavirus, West Nile virus and zika virus.
  • HlV human immunodeficiency virus
  • AIDS human immunodeficiency virus
  • EBV Epstein-Barr virus
  • CMV cytomegalovirus
  • Herpes simplex virus infection yellow fever
  • Ebola virus infection rabies
  • bacterial infections which may be treated according to the teachings of the present invention include, but are not limited to, those caused by anthrax; gram-negative bacilli, chlamydia, diptheria, haemophilus influenza, Helicobacter pylori, malaria, Mycobacterium tuberculosis, pertussis toxin, pneumococcus, rickettsiae, staphylococcus, streptococcus and tetanus.
  • superbug infections e.g. multi-drug resistant bacteria
  • superbug infections include, but are not limited to, those caused by Enterococcus faecium, Clostridium difficile, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacteriaceae (including Escherichia coli, Klebsiella pneumoniae, Enterobacter spp).
  • fungal infections which may be treated according to the teachings of the present invention include, but are not limited to, those caused by Candida, coccidiodes, cryptococcus, histoplasma, leishmania, plasmodium, protozoa, parasites, schistosomae, tinea, toxoplasma, and trypanosoma cruzi.
  • the disease is associated with transplantation of a graft.
  • diseases associated with transplantation of a graft include, but are not limited to, graft rejection, chronic graft rejection, subacute graft rejection, hyperacute graft rejection, acute graft rejection, allograft rejection, xenograft rejection and graft-versus-host disease (GVHD).
  • graft rejection chronic graft rejection
  • subacute graft rejection hyperacute graft rejection
  • acute graft rejection allograft rejection
  • xenograft rejection graft-versus-host disease
  • Non-malignant hematologic diseases include, but are not limited to, anemia, bone marrow disorders, deep vein thrombosis/pulmonary embolism, diamond blackfan anemia, hemochromatosis, hemophilia, immune hematologic disorders, iron metabolism disorders, sickle cell disease (e.g. sickle cell anemia), thalassemia, thrombocytopenia and Von Willebrand disease.
  • anemia bone marrow disorders
  • deep vein thrombosis/pulmonary embolism diamond blackfan anemia
  • hemochromatosis hemophilia
  • immune hematologic disorders iron metabolism disorders
  • sickle cell disease e.g. sickle cell anemia
  • thalassemia thalassemia
  • thrombocytopenia Von Willebrand disease.
  • the non-malignant disease is a sickle cell disease or an autoimmune disease (e.g. treatable by HCT plus veto cells, as discussed below).
  • Non-limiting examples of autoimmune diseases include, but are not limited to, Acute disseminated encephalomyelitis (ADEM), Addison's disease, Agammaglobulinemia, Alopecia areata, Amyotrophic lateral sclerosis, Ankylosing Spondylitis, Antiphospholipid syndrome, Antisynthetase syndrome, Atopic allergy, Atopic dermatitis, Autoimmune aplastic anemia, Autoimmune cardiomyopathy, Autoimmune enteropathy, Autoimmune hemolytic anemia, Autoimmune hepatitis, Autoimmune inner ear disease, Autoimmune lymphoproliferative syndrome, Autoimmune peripheral neuropathy, Autoimmune pancreatitis, Autoimmune polyendocrine syndrome, Autoimmune progesterone dermatitis, Autoimmune thrombocytopenic purpura, Autoimmune uveitis (e.g.
  • Non-infectious uveitis Behcet's disease, Celiac disease, Chronic Glomerulonephritis, Cold agglutinin disease, Crohn's disease, Dermatomyositis, Dermatomyositis, Diabetes mellitus type 1 (Type 1 diabetes mellitus (T1DM)), Eosinophilic fasciitis, Goodpasture's syndrome, Graves' disease, Guillain-Barre syndrome (GBS), Hashimoto's encephalopathy, Hashimoto's thyroiditis, Idiopathic thrombocytopenic purpura, Immune Thrombocytic Purpura (ITP), Lupus erythematosus (SLE), Miller-Fisher syndrome, Mixed connective tissue disease, Multiple sclerosis (MS), Myasthenia gravis, Narcolepsy, Pemphigus vulgaris, Pernicious anaemia, Polymyositis Primary biliary cirrhosis, Psoriasis, Psori
  • the autoimmune disease is Lupus erythematosus (SLE), Multiple sclerosis (MS) and Diabetes mellitus type 1 (Type 1 diabetes mellitus (T1DM)).
  • SLE Lupus erythematosus
  • MS Multiple sclerosis
  • T1DM Diabetes mellitus type 1
  • the non-malignant disease is an aplastic anemia, a severe immune deficiency and a non-malignant bone marrow failure.
  • the non-GVHD inducing cells comprising a Tcm phenotype may be selected from an antigen or antigens (e.g. viral antigens) associated with the disease to be treated and to generate antigen specific Tcm cells for treatment. Additionally or alternatively, as discussed above, the non-GVHD inducing cells comprising a Tcm phenotype may be genetically modified for therapy.
  • an antigen or antigens e.g. viral antigens
  • the non-GVHD inducing cells comprising a Tcm phenotype may be genetically modified for therapy.
  • the non-GVHD inducing cells comprising a Tcm phenotype of the invention are endowed with veto activity. Accordingly, the Tcm cells of the present invention may be used as adjuvant therapy for a cell or tissue transplant. As the non-GVHD inducing cells comprising a Tcm phenotype of the present invention are also endowed with anti-disease activity the method of the present invention can furthermore be advantageously applied towards treating a disease in a subject while concomitantly facilitating engraftment of a transplant of cells or tissues .
  • the method further comprises transplanting a cell or tissue transplant into a subject.
  • the medicament further comprises a cell or tissue transplant.
  • a method of treating a subject in need of a non-syngeneic cell or tissue transplant comprising: (a) transplanting a cell or tissue transplant into the subject; and (b) administering to the subject a therapeutically effective amount of the isolated population of non-GVHD inducing cells of some embodiments of the invention, thereby treating the subject in need of the cell or tissue transplant.
  • tissue transplant refers to a bodily cell (e.g. a single cell or a group of cells) or tissue (e.g. solid tissues/organs or soft tissues, which may be transplanted in full or in part).
  • tissue e.g. solid tissues/organs or soft tissues, which may be transplanted in full or in part.
  • Exemplary tissues or organs which may be transplanted according to the present teachings include, but are not limited to, liver, pancreas, spleen, kidney, heart, lung, skin, intestine and lymphoid/hematopoietic tissues (e.g. lymph node, Peyer’s patches thymus or bone marrow).
  • Exemplary cells which may be transplanted according to the present teachings include, but are not limited to, immature hematopoietic cells, including stem cells, cardiac cells, hepatic cells, pancreatic cells, spleen cells, pulmonary cells, brain cells, nephric cells, intestine/gut cells, ovarian cells, skin cells, (e.g. isolated population of any of these cells).
  • immature hematopoietic cells including stem cells, cardiac cells, hepatic cells, pancreatic cells, spleen cells, pulmonary cells, brain cells, nephric cells, intestine/gut cells, ovarian cells, skin cells, (e.g. isolated population of any of these cells).
  • the present invention also contemplates transplantation of whole organs, such as for example, kidney, heart, liver or skin.
  • the cells or tissues of the present invention may be obtained from a prenatal organism, postnatal organism, an adult organism or a cadaver donor. Moreover, depending on the application needed the cells or tissues may be naive or genetically modified. Such determinations are well within the ability of one of ordinary skill in the art.
  • Any method known in the art may be employed to obtain a cell or tissue (e.g. for transplantation).
  • the cell or tissue transplant comprises immature hematopoietic cells.
  • the phrase “immatopoietic cells” refers to a hematopoietic tissue or cell preparation comprising precursor hematopoietic cells (e.g. hematopoietic stem cells).
  • tissue/cell preparation includes or is derived from a biological sample, for example, bone marrow, mobilized peripheral blood (e.g. mobilization of CD34 + cells to enhance their concentration), cord blood (e.g. umbilical cord), fetal liver, yolk sac and/or placenta.
  • purified CD34 + cells or other hematopoietic stem cells such as CD131 + cells can be used in accordance with the present teachings, either with or without ex-vivo expansion.
  • the immature hematopoietic cells comprise T cell depleted immature hematopoietic cells.
  • T cell depleted immature hematopoietic cells refers to a population of precursor hematopoietic cells which are depleted of T lymphocytes.
  • the T cell depleted immature hematopoietic cells may include e.g. CD34 + , CD33 + and/or CD56 + cells.
  • the T cell depleted immature hematopoietic cells may be depleted of CD3 + cells, CD2 + cells, CD8 + cells, CD4 + cells, a/b T cells and/or g/d T cells.
  • the immature hematopoietic cells comprise T cell depleted mobilized blood cells enriched for CD34 + immature hematopoietic cells.
  • the T cell depleted immature hematopoietic cells comprise at least about 0.1 x 10 6 CD34 + cells, 0.5 x 10 6 CD34 + cells, 1 x 10 6 CD34 + cells, 2 x 10 6 CD34 + cells, 3 x 10 6 CD34 + cells, 4 x 10 6 CD34 + cells, 5 x 10 6 CD34 + cells, 6 x 10 6 CD34 + cells, 7 x 10 6 CD34 + cells, 8 x 10 6 CD34 + cells, 9 x 10 6 CD34 + cells, 10 x 10 6 CD34 + cells, 15 x 10 6 CD34 + cells or 20 x 10 6 CD34 + cells per kg ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise at least about 2.5-20 x 10 6 CD34 + cells (e.g. 5-10 x 10 6 CD34 + cells) per kg ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise at least about 5 x 10 6 CD34 + cells per kg ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise at least about 6 x 10 6 CD34 + cells per kg ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise at least about 8 x 10 6 CD34 + cells per kg ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise at least about 10 x 10 6 CD34 + cells per kg ideal body weight of the subject.
  • the immature hematopoietic cells are depleted of CD3 + and/or CD19 + cells.
  • the T cell depleted immature hematopoietic cells comprise less than about 50 x 10 5 CD3 + cells, 40 x 10 5 CD3 + cells, 30 x 10 5 CD3 + cells, 20 x 10 5 CD3 + cells, 15 x 10 5 CD3 + cells, 10 x 10 5 CD3 + cells, 9 x 10 5 CD3 + cells, 8 x 10 5 CD3 + cells, 7 x 10 5 CD3 + cells, 6 x 10 5 CD3 + cells, 5 x 10 5 CD3 + cells, 4 x 10 5 CD3 + cells, 3 x 10 5 CD3 + cells, 2 x 10 5 CD3 + cells, 1 x 10 5 CD3 + cells, 0.5 x 10 5 CD3 + cells or 0.1 x 10 5 CD3 + cells per kilogram ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than 1-5 x 10 5 CD3 + cells (e.g. 2-5 x 10 5 CD3 + cells)
  • the T cell depleted immature hematopoietic cells comprise less than 5 x 10 5 CD3 + cells per kilogram ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than 4 x 10 5 CD3 + cells per kilogram ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than 3 x 10 5 CD3 + cells per kilogram ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than 2 x 10 5 CD3 + cells per kilogram ideal body weight of the subject.
  • the immature hematopoietic cells are depleted of CD8+ cells.
  • the T cell depleted immature hematopoietic cells comprise less than 1 x 10 4 - 5 x 10 5 CD8 + cells (e.g. 0.1-4 x 10 5 CD8 + cells, e.g. 1-3 x 10 5 CD8 + cells) per kilogram ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than about 50 x 10 5 CD8 + cells, 25 x 10 5 CD8 + cells, 15 x 10 5 CD8 + cells, 10 x 10 5 CD8 + cells, 9 x 10 5 CD8 + cells, 8 x 10 5 CD8 + cells, 7 x 10 5 CD8 + cells, 6 x 10 5 CD8 + cells, 5 x 10 5 CD8 + cells,
  • the T cell depleted immature hematopoietic cells comprise less than 5 x 10 5 CD8 + cells per ideal kilogram body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than 4 x 10 5 CD8 + cells per ideal kilogram body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than 3 x 10 5 CD8 + cells per ideal kilogram body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than about 1 x 10 6 CD8 + TCRo/b- cells, 0.5 x 10 6 CD8 + TCRa/b- cells, 1 x 10 5 CD8 + TCRa/b- cells, 0.5 x 10 5 CD8 + TCRa/b- cells, 1 x 10 4 CD8 + TCRa/b- cells, 0.5 x 10 4 CD8 + TCRa/b- cells, 1 x 10 3 CD8 + TCRa/b- cells or 0.5 x 10 3 CD8 + TCRa/b- cells per kilogram ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than 1 x 10 5 - 1 x 10 6 CD8 + TCRa/b- cells per kilogram ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than 1 x 10 6 CD8 + TCRa/b- cells per kilogram ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than 5 x 10 5 CD8 + TCRa/b- cells per kilogram ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise less than 1 x 10 5 CD8 + TCRa/b- cells per kilogram ideal body weight of the subject. According to one embodiment, the immature hematopoietic cells are depleted of B cells.
  • the immature hematopoietic cells are depleted of B cells (CD19 + and/or CD20 + B cells).
  • the immature hematopoietic cells comprise less than about 50 x 10 5 CD19 + and/or CD20 + cells, 40 x 10 5 CD19 + and/or CD20 + cells, 30 x 10 5 CD19 + and/or CD20 + cells, 20 x 10 5 CD19 + and/or CD20 + cells, 10 x 10 5 CD19 + and/or CD20 + cells, 9 x 10 5
  • the immature hematopoietic cells comprise less than
  • the immature hematopoietic cells comprise less than 4 x 10 5 CD19 + and/or CD20 + cells per kilogram ideal body weight of the subject. According to a specific embodiment, the immature hematopoietic cells comprise less than 3 x 10 5 CD19 + and/or CD20 + cells per kilogram ideal body weight of the subject.
  • the immature hematopoietic cells comprise less than 2 x 10 5 CD19 + and/or CD20 + cells per kilogram ideal body weight of the subject.
  • the T cell depleted immature hematopoietic cells comprise two or more batches of cells, e.g. a first batch comprising CD34+ selected cells and a second batch comprising CD3 + /CD19 + -depleted cells (i.e. obtained from the same donor). It will be appreciated that these can be used concomitantly or subsequent to each other (e.g. on the same day or within e.g. about 1, 2, 3, 4, 5, 6, 7 days of each other, as discussed below). Depletion of T cells, e.g. CD3 + , CD2 + , TCRa/b , CD4 + and/or CD8 + cells, or B cells, e.g.
  • CD19 + and/or CD20 + cells may be carried out using any method known in the art, such as by eradication (e.g. killing) with specific antibodies or by affinity based purification e.g. such as by the use of magnetic cell separation techniques, FACS sorter and/or capture ELISA labeling.
  • eradication e.g. killing
  • affinity based purification e.g. such as by the use of magnetic cell separation techniques, FACS sorter and/or capture ELISA labeling.
  • FACS fluorescence activated cell sorting
  • Any ligand-dependent separation techniques known in the art may be used in conjunction with both positive and negative separation techniques that rely on the physical properties of the cells rather than antibody affinity, including but not limited to elutriation and density gradient centrifugation.
  • cell sorting include, for example, panning and separation using affinity techniques, including those techniques using solid supports such as plates, beads and columns.
  • affinity techniques including those techniques using solid supports such as plates, beads and columns.
  • biological samples may be separated by "panning" with an antibody attached to a solid matrix, e.g. to a plate.
  • cells may be sorted/separated by magnetic separation techniques, and some of these methods utilize magnetic beads.
  • Different magnetic beads are available from a number of sources, including for example, Dynal (Norway), Advanced Magnetics (Cambridge, MA, U.S.A.), Immuncon (Philadelphia, U.S.A.), Immunotec (Marseille, France), Invitrogen, Stem cell Technologies (U.S.A) and Cellpro (U.S.A).
  • antibodies can be biotinylated or conjugated with digoxigenin and used in conjunction with avidin or anti-digoxigenin coated affinity columns.
  • different depletion/separation methods can be combined, for example, magnetic cell sorting can be combined with FACS, to increase the separation quality or to allow sorting by multiple parameters.
  • T cell depleted immature hematopoietic cells are obtained by a method comprising collecting mobilized PBMCs from a donor subject (e.g. the same donor subject from which non-mobilized PBMCs were collected for generation of non-GVHD inducing cells comprising a Tcm phenotype).
  • mobilization is affected by G-CSF.
  • mobilization is affected by G-CSF and plerixafor.
  • the collection of mobilized PBMCs is obtained in a single collection.
  • the collection of the mobilized PBMCs is obtained in two, three, four, five or more daily collection, e.g. three daily collections (e.g. on consequent days or within a few days apart).
  • a back-up fraction of unmodified mobilized PBMC containing at least about 0.1-5 x 10 6 CD34 + cells, e.g. 2 x 10 6 CD34 + cells, e.g. 1 x 10 6 CD34 + cells per kg ideal body weight (e.g. obtained from the first collection) is set aside and cryopreserved.
  • the collections of mobilized PBMCs are pooled, CD34 + selected (as discussed below) and cryopreserved.
  • the third day’s collection is depleted of CD3 + /CD19 + cells (as discussed below) and cryopreserved.
  • enrichment of CD34 + expressing cells is affected by incubating the PBMCs with a CD34 binding agent.
  • the CD34 binding agent is an antibody.
  • the CD34 antibody is a monoclonal antibody.
  • the CD34 monoclonal antibody is conjugated to magnetic particles. According to a specific embodiment, the CD34 monoclonal antibody is conjugated to super- paramagnetic particles.
  • the CD34 + labeled cells are selected by magnetic separation techniques (as discussed in detail hereinabove).
  • the CD34 magnetically labeled cells i.e. CD34 + expressing cells
  • the separation column i.e. positive selection
  • the CD34- cells are removed.
  • the CD34 + cells are then released from the column and collected.
  • samples from each fraction are removed for cell count, viability and/or immunopheno typing .
  • the mobilized PBMCs are depleted of platelets using e.g. COBE 2991.
  • the post-platelet depleted PBMC preparation of one embodiment is incubated with IVIg for 5-30 minutes e.g. 10-15 minutes. After the initial incubation the CD3 + and CD19 + binding agents are added to the cell preparation and incubated for e.g. 10-60 minutes, e.g. 30 minutes, on an orbital rotator.
  • the CD3 and/or CD 19 binding agent is an antibody.
  • the CD3 and/or CD 19 antibody is a monoclonal antibody.
  • the CD3 and/or CD 19 monoclonal antibody is conjugated to magnetic particles.
  • the CD3 and/or CD 19 monoclonal antibody is conjugated to super-paramagnetic particles.
  • the cells are washed by centrifugation and the cell pellet resuspended in buffer (e.g. COBE 2991) to remove excess reagent.
  • the CD3 + /CD19 + labeled cells are selected by magnetic separation techniques (as discussed in detail hereinabove).
  • the CD3 + /CD19 + labeled cells are processed on CliniMACS ® column.
  • the CD3 + /CD19 + magnetically labeled cells i.e. CD3 + /CD19 + expressing cells
  • the separation column i.e. negative selection
  • the CD4-/CD56-/CD45RA- cells are collected.
  • the collected cells CD3VCD19- cells
  • samples from each fraction are removed for cell count, viability and/or immunopheno typing.
  • a second CD3 + depletion step is carried out (as discussed above) in situations in which more than about 1 x 10 5 CD3 to 5 x 10 5 CD3, e.g. 1 x 10 5 CD3 to 3 x 10 5 CD3, e.g. 2.5 x 10 5 CD3, e.g. 2 x 10 5 CD3, per kg ideal body weight are present in the collected cell fraction.
  • the T cell depleted immature hematopoietic cells i.e. for cell transplantation
  • the PBMCs used for generation of the non-GVHD inducing cells comprising a Tcm phenotype are obtained from the same donor subject.
  • the method may be affected using a cell or tissue which is syngeneic or non-syngeneic with the recipient subject (e.g. allogeneic), as discussed hereinabove.
  • both the recipient subject and the donor subject are humans.
  • the subject i.e. recipient subject
  • the subject i.e. recipient subject
  • the subject i.e. recipient subject
  • a disease which can be treated by immature hematopoietic cell transplantation e.g. T cell depleted immature hematopoietic cells.
  • the subject i.e. recipient subject
  • a solid organ or tissue e.g. kidney, pulmonary, liver, pancreatic, cardiac, etc.
  • the subject i.e. recipient subject
  • a solid organ or tissue e.g. kidney, pulmonary cells, liver cells, pancreatic cells, cardiac cells, etc. as discussed above.
  • the subject i.e. recipient subject
  • organs e.g. cardiac and pulmonary tissues
  • the cells, organs or tissues for co-transplantation are obtained from the same donor.
  • Transplanting the cell or tissue into the subject may be affected in numerous ways, depending on various parameters, such as, for example, the cell or tissue type; the type, stage or severity of the recipient's disease (e.g. type and stage of malignant disease); the physical or physiological parameters specific to the subject; and/or the desired therapeutic outcome.
  • the cell or tissue type e.g., the cell or tissue type; the type, stage or severity of the recipient's disease (e.g. type and stage of malignant disease); the physical or physiological parameters specific to the subject; and/or the desired therapeutic outcome.
  • Transplanting a cell or tissue transplant of the present invention may be affected by transplanting the cell or tissue transplant into any one of various anatomical locations, depending on the application.
  • the cell or tissue transplant may be transplanted into a homotopic anatomical location (a normal anatomical location for the transplant), or into an ectopic anatomical location (an abnormal anatomical location for the transplant).
  • the cell or tissue transplant may be advantageously implanted under the renal capsule, or into the kidney, the testicular fat, the sub cutis, the omentum, the portal vein, the liver, the spleen, the heart cavity, the heart, the chest cavity, the lung, the skin, the pancreas and/or the intra- abdominal space.
  • the immature hematopoietic cells may be transplanted into a subject using any method known in the art for cell transplantation, such as but not limited to, cell infusion (e.g. I.V.) or via an intraperitoneal route.
  • cell infusion e.g. I.V.
  • intraperitoneal route e.g. I.V.
  • the cell numbers of immature hematopoietic cells can be monitored in a subject by standard blood and bone marrow tests (e.g. by FACS analysis). Structural development of the cells or tissues may be monitored via computerized tomography, or ultrasound imaging.
  • the method may further advantageously comprise conditioning the subject under sublethal, lethal or supralethal conditions prior to the transplanting .
  • the terms “sublethal”, “lethal”, and “supralethal”, when relating to conditioning of subjects of the present invention, refer to myelotoxic and/or lymphocytotoxic treatments which, when applied to a representative population of the subjects, respectively, are typically: non-lethal to essentially all members of the population; lethal to some but not all members of the population; or lethal to essentially all members of the population under normal conditions of sterility.
  • the sublethal, lethal or supralethal conditioning comprises a total body irradiation (TBI), total lymphoid irradiation (TLI, i.e. exposure of all lymph nodes, the thymus, and spleen), partial body irradiation (e.g. specific exposure of the lungs, kidney, brain etc.), myeloablative conditioning and/or non-myeloablative conditioning, e.g. with different combinations including, but not limited to, co- stimulatory blockade, chemotherapeutic agent and/or antibody immunotherapy.
  • the conditioning comprises a combination of any of the above described conditioning protocols (e.g. chemotherapeutic agent and TBI, co-stimulatory blockade and chemotherapeutic agent, antibody immunotherapy and chemotherapeutic agent, etc).
  • the conditioning is affected by conditioning the subject under supralethal conditions, such as under myeloablative conditions (i.e. intensive conditioning regimen in which the bone marrow cells are destroyed).
  • supralethal conditions such as under myeloablative conditions (i.e. intensive conditioning regimen in which the bone marrow cells are destroyed).
  • the conditioning may be affected by conditioning the subject under lethal or sublethal conditions, such as by conditioning the subject under myeloreductive conditions or non- myeloablative conditions, respectively (i.e. reduced intensity conditioning which is a less aggressive conditioning regimen).
  • the conditioning comprises non-myeloablative conditioning (e.g. a reduced intensity conditioning regimen).
  • the reduced intensity conditioning is affected for up to 2 weeks (e.g. 1-10 or 1-7 days) prior to transplantation of the cell or tissue graft.
  • the non-myeloablative conditioning comprises a chemotherapeutic agent and TBI/TLI.
  • the TBI comprises a single or fractionated irradiation dose within the range of 0.5-1 Gy, 0.5-1.5 Gy, 0.5-2 Gy, 0.5-2.5 Gy, 0.5-5 Gy, 0.5-7.5 Gy, 0.5-10 Gy, 0.5-15 Gy, 1-1.5 Gy, 1-2 Gy, 1-2.5 Gy, 1-3 Gy, 1-3.5 Gy, 1-4 Gy, 1-4.5 Gy, 1-5 Gy, 1-7.5 Gy, 1-10 Gy, 2-3 Gy, 2-4 Gy, 2-5 Gy, 2-6 Gy, 2-7 Gy, 2-8 Gy, 2-9 Gy, 2-10 Gy, 3-4 Gy, 3-5 Gy, 3-6 Gy, 3-7 Gy, 3-8 Gy, 3-9 Gy, 3-10 Gy, 4-5 Gy, 4-6 Gy, 4-7 Gy, 4-8 Gy, 4-9 Gy, 4-10 Gy, 5-6 Gy, 5-7 Gy, 5- 8 Gy, 5-9 Gy, 5-10 Gy, 6-7 Gy, 6-8 Gy, 6-9 Gy, 6-10 Gy, 7-8 Gy, 7-9 Gy, 7-10 Gy, 8-9 Gy, 8-10 Gy, 10-12 Gy or 10-15 Gy.
  • the TBI comprises a single or fractionated irradiation dose within the range of 1-7.5 Gy.
  • the TBI comprises a single or fractionated irradiation dose within the range of 1-5 Gy.
  • the TBI comprises a single or fractionated irradiation dose of 3 Gy.
  • TBI treatment is administered to the subject 1-10 days (e.g. 1-3 days) prior to transplantation.
  • the subject is conditioned once with TBI 1, 2, 3 or 4 days prior to transplantation.
  • TBI is administered on day -2 prior to transplantation.
  • TBI is administered on day -1 prior to transplantation.
  • TBI is administered on the day of transplantation, e.g. in the morning of day 0, and transplantation is carried out on the same day, e.g. in the evening.
  • the TBI comprises a single or fractionated irradiation dose on day -1 (i.e. one day prior to transplantation).
  • the TLI comprises an irradiation dose within the range of 0.5-1 Gy, 0.5-1.5 Gy, 0.5-2.5 Gy, 0.5-5 Gy, 0.5-7.5 Gy, 0.5-10 Gy, 0.5-15 Gy, 1-1.5 Gy, 1-2 Gy, 1-2.5 Gy, 1-3 Gy, 1-3.5 Gy, 1-4 Gy, 1-4.5 Gy, 1-1.5 Gy, 1-7.5 Gy, 1-10 Gy, 2-3 Gy, 2-4 Gy, 2-5 Gy, 2-6 Gy, 2-7 Gy, 2-8 Gy, 2-9 Gy, 2-10 Gy, 3-4 Gy, 3-5 Gy, 3-6 Gy, 3-7 Gy, 3-8 Gy, 3-9 Gy, 3- 10 Gy, 4-5 Gy, 4-6 Gy, 4-7 Gy, 4-8 Gy, 4-9 Gy, 4-10 Gy, 5-6 Gy, 5-7 Gy, 5-8 Gy, 5-9 Gy, 5-10 Gy, 6-7 Gy, 6-8 Gy, 6-9 Gy, 6-10 Gy, 7-8 Gy, 7-9 Gy, 7-10 Gy, 8-9 Gy, 8-10 Gy, 10-12 Gy, 10-15 Gy, 10-20 Gy, 10-30 Gy, 10
  • the TLI comprises a single or fractionated irradiation dose within the range of 1-5 Gy.
  • the TLI comprises a single or fractionated irradiation dose of 3 Gy.
  • TLI treatment is administered to the subject 1-10 days (e.g. 1- 3 days) prior to transplantation.
  • the subject is conditioned once with TLI 1, 2, 3 or 4 days prior to transplantation.
  • TLI is administered on day -2 prior to transplantation.
  • TLI is administered on day -1 prior to transplantation.
  • TLI is administered on the day of transplantation, e.g. in the morning of day 0, and transplantation is carried out on the same day, e.g. in the evening.
  • the TLI comprises a single or fractionated irradiation dose on day -1 (i.e. one day prior to transplantation).
  • the conditioning comprises a chemotherapeutic agent.
  • chemotherapeutic agents include, but are not limited to, Busulfan, Busulfex, Cyclophosphamide, Fludarabine, Melphalan, Myleran, Rapamycin, Trisulphan, and Thiotepa.
  • the chemotherapeutic agent/s may be administered to the subject in a single dose or in several doses e.g. 2, 3, 4, 5, 6, 7, 8, 9, 10 or more doses (e.g. daily doses) prior to transplantation.
  • the subject is administered a chemotherapeutic agent (e.g. Fludarabine e.g. at a dose of about 30 mg/m 2 /day) for 3, 4, 5 or 6 consecutive days (e.g. 4 consecutive days) prior to transplantation (e.g. on days -7 to -4, e.g. on days -6 to -3).
  • a chemotherapeutic agent e.g. Fludarabine e.g. at a dose of about 30 mg/m 2 /day
  • Fludarabine is commercially available from e.g. Sanofi Genzyme, Bayer and Teva, e.g. under the brand name e.g. Fludara.
  • the pre-transplant conditioning comprises in vivo T cell debulking.
  • the in-vivo T cell debulking is affected by antibodies.
  • the antibodies comprise an anti-CD8 antibody, an anti-CD4 antibody, or both.
  • the antibodies comprise anti-thymocyte globulin (ATG) antibodies, anti-CD52 antibodies or anti-CD3 (OKT3) antibodies.
  • ATG anti-thymocyte globulin
  • the antibody is administered to the subject in a single dose or in several doses e.g. 2, 3, 4, 5, 6, 7, 8, 9, 10 or more doses (e.g. daily doses) prior to transplantation.
  • the subject is administered an antibody therapeutic agent (e.g. ATG e.g. at a dose of about 2 mg per kg ideal body weight) for 2, 3, 4 or 5 consecutive days (e.g. 3 consecutive days) prior to transplantation (e.g. on days -10 to -8 prior to transplantation, e.g. on days -9 to -7 prior to transplantation).
  • an antibody therapeutic agent e.g. ATG e.g. at a dose of about 2 mg per kg ideal body weight
  • the subject is treated with three administrations of ATG prior to transplantation (e.g. at a dose of about 2 mg per kg ideal body weight, e.g. on days -9, -8 and -7 prior to transplantation).
  • the subject is treated with two administrations of ATG prior to transplantation (e.g. at a dose of about 2 mg per kg ideal body weight, e.g. on days -9 and - 8, or -8 and -7 prior to transplantation).
  • the subject is treated with a single administration of ATG prior to transplantation (e.g. at a dose of about 2 mg per kg ideal body weight, e.g. on days -9, -8 or -7 prior to transplantation).
  • a single administration of ATG prior to transplantation e.g. at a dose of about 2 mg per kg ideal body weight, e.g. on days -9, -8 or -7 prior to transplantation.
  • the pre-transplant conditioning does not comprise in vivo T cell debulking.
  • the subject is not treated with ATG prior to transplantation.
  • Anti-thymocyte globulin (ATG) antibodies are commercially available from e.g. Genzyme and Pfizer, e.g. under the brand names e.g. Thymoglobulin and Atgam.
  • the pre-transplant conditioning regimen comprises rituximah (e.g. at a dose of about 375 mg/m 2 , e.g. on days -12, -11, -10, -9, -8 or -7, e.g. on day -10 prior to transplantation).
  • Rituximab is commercially available from e.g. Genentech and Roche, e.g. under the brand names e.g. Mabthera, Rixathon, Truxima, Rituxan.
  • the method comprises post-transplant administration of a therapeutically effective amount of cyclophosphamide.
  • the present invention further contemplates administration of cyclophosphamide prior to transplantation (e.g. on days 6, 5, 4 or 3 prior to transplantation, i.e. D-6 to -3) in addition to the administration following transplantation as described herein.
  • the therapeutic effective amount of cyclophosphamide comprises about 1-25 mg, 1-50 mg, 1-75 mg, 1-100 mg, 1-250 mg, 1-500 mg, 1-750 mg, 1-1000 mg, 5-50 mg, 5-75 mg, 5-100 mg, 5-250 mg, 5-500 mg, 5-750 mg, 5-1000 mg, 10-50 mg, 10-75 mg, 10-100 mg, 10-250 mg, 10-500 mg, 10-750 mg, 10-1000 mg, 25-50 mg, 25- 75 mg, 25-100 mg, 25-125 mg, 25-200 mg, 25-300 mg, 25-400 mg, 25-500 mg, 25-750 mg, 25- 1000 mg, 50-75 mg, 50-100 mg, 50-125 mg, 50-150 mg, 50-175 mg, 50-200 mg, 50-250 mg, 50- 500 mg, 50-1000 mg, 75-100 mg, 75-125 mg, 75-150 mg, 75-250 mg, 75-500 mg, 75-1000 mg, 100-125 mg, 100-150 mg, 100-200 mg,
  • the therapeutic effective amount of cyclophosphamide is about 25-200 mg per kilogram ideal body weight of the subject.
  • cyclophosphamide is administered in a single dose.
  • cyclophosphamide is administered in multiple doses, e.g. in 2, 3, 4, 5 doses or more.
  • cyclophosphamide is administered in two doses.
  • each cyclophosphamide administration may comprise about 5 mg, 7.5 mg, 10 mg, 20 mg, 30 mg, 40 mg, 50 mg, 60 mg, 70 mg, 80 mg, 90 mg, 100 mg, 110 mg, 120 mg, 130 mg, 140 mg, 150 mg, 160 mg, 170 mg, 180 mg, 190 mg, 200 mg, 210 mg, 220 mg, 230 mg, 240 mg, 250 mg, 260 mg, 270 mg, 280 mg, 290 mg, 300 mg, 350 mg, 400 mg, 450 mg or 500 mg per kilogram ideal body weight of the subject.
  • each dose of cyclophosphamide is 50 mg per kilogram ideal body weight of the subject.
  • cyclophosphamide is administered post transplantation.
  • cyclophosphamide may be administered to the subject 1, 2, 3, 4, 5 days post-transplant (i.e., D+l, +2, +3, +4, +5).
  • cyclophosphamide is administered to the subject in two doses 3 and 4 days post-transplant.
  • Cyclophosphamide is commercially available from e.g. Zydus (German Remedies), Roxane Laboratories Inc-Boehringer Ingelheim, Bristol-Myers Squibb Co - Mead Johnson and Co, and Pfizer - Pharmacia & Upjohn, under the brand names of Endoxan, Cytoxan, Neosar, Procytox and Revimmune.
  • the subject is treated with additional supportive drugs, e.g. chemotherapy adjuvants.
  • additional supportive drugs e.g. chemotherapy adjuvants.
  • the subject is treated with a dose of Mesna (e.g. 10 mg/kg intravenous piggy back (IVPB) just prior to the first dose of cyclophosphamide (e.g. 2 hours, 1 hour, 30 minutes, 15 minutes prior to the first dose of cyclophosphamide).
  • a dose of Mesna e.g. 10 mg/kg intravenous piggy back (IVPB) just prior to the first dose of cyclophosphamide (e.g. 2 hours, 1 hour, 30 minutes, 15 minutes prior to the first dose of cyclophosphamide).
  • IVPB intravenous piggy back
  • administration of mesna is repeated every 4 hours for a total of 10 doses.
  • Mesna is commercially available from e.g. Baxter under the brand names of Uromitexan and Mesnex.
  • the subject is treated with ondansetron (or another anti- emetic) prior to each dose of Cyclophosphamide (Cy).
  • the subject is not treated with an immunosuppressive agent (e.g. aside from the CY and veto cells discussed herein).
  • an immunosuppressive agent e.g. aside from the CY and veto cells discussed herein.
  • the subject is treated with an immunosuppressive agent.
  • immunosuppressive agents include, but are not limited to, Tacrolimus (also referred to as FK-506 or fujimycin, trade names: Prograf, Advagraf, Protopic), Mycophenolate Mofetil, Mycophenolate Sodium, Prednisone, methotrexate, cyclophosphamide, cyclosporine, cyclosporin A, chloroquine, hydroxychloroquine, sulfasalazine (sulphasalazopyrine), gold salts, D- penicillamine, leflunomide, azathioprine, anakinra, infliximab (REMICADE), etanercept, TNF.alpha.
  • Tacrolimus also referred to as FK-506 or fujimycin, trade names: Prograf, Advagraf, Protopic
  • Mycophenolate Mofetil Mycophenolate Sodium
  • Prednisone methotrexate
  • cyclophosphamide
  • NSAIDs Non-Steroidal Anti-Inflammatory Drug
  • NSAIDs include, but are not limited to acetyl salicylic acid, choline magnesium salicylate, difhmisal, magnesium salicylate, salsalate, sodium salicylate, diclofenac, etodolac, fenoprofen, flurbiprofen, indomethacin, ketoprofen, ketorolac, meclofenamate, naproxen, nabumetone, phenylbutazone, piroxicam, sulindac, tolmetin, acetaminophen, ibuprofen, Cox-2 inhibitors, tramadol, rapamycin (sirolimus) and rapamycin analogs (such as CCI-779, RAD001, AP23573). These agents may be administered individually or in combination.
  • corticosteroids are not administered as a pretreatment to the veto cells.
  • a method of treating a subject in need of an immature hematopoietic cell transplantation comprising:
  • non-myeloablative pre-transplant conditioning protocol comprising a total body irradiation (TBI) and a chemotherapeutic agent, wherein the TBI and the chemotherapeutic agent are administered on days -7 to -1 prior to transplantation (e.g. on days -7 to -2, or on days -6 to -1 prior to transplantation);
  • TBI total body irradiation
  • chemotherapeutic agent e.g. on days -7 to -2, or on days -6 to -1 prior to transplantation
  • T cell depleted immature hematopoietic cells comprises less than 5 x 10 5 CD3 + T cells per kilogram ideal body weight of the subject, and wherein the T cell depleted immature hematopoietic cells comprise at least 5 x 10 6 CD34 + cells per kilogram ideal body weight of the subject;
  • TBI is administered on the day of transplantation of the T cell depleted immature hematopoietic cells e.g. in the morning of day 0, and transplantation is carried out on the same day, e.g. in the evening.
  • the subject is treated as follows: Fludarabine is administered to the subject in 4 consequetive doses (e.g. 30 mg/m 2 /day) on days -6 to -3 (prior to transplanation), TBI is administered to the subject at a single or fractionated irradiation dose (e.g. of 3-5 Gy, e.g. 3 Gy) on day -1 prior to transplantation, on day 0 (i.e. transplantation day) the subject is infused (e.g. IV) with megadose T cell depleted immature hematopoietic cells comprising CD34 + selected cells and/or CD3/CD19-depleted cells (e.g.
  • the subject is administered (e.g. IV) with Cyclophosphamide (CY) each at a dose of e.g. 50 mg/kg ideal body weight/day, and on day +7 the subject is infused with the anti-viral veto cells of some embodiments ofo the invention at a dose of e.g. 2.5-10 x 10 6 CD8 + cells per kg ideal body weight.
  • CY Cyclophosphamide
  • the subject is administed with ATG (Thymoglobulin) in 3 consequetive doses (e.g. 2 mg per kg ideal body weight) on days -9 to -7 (prior to transplanation).
  • the subject is treated as follows: Fludarabine is administered to the subject in 4 consequetive doses (e.g. 30 mg/m 2 /day) on days -7 to -4 (prior to transplanation), TBI is administered to the subject at a single or fractionated irradiation dose (e.g. of 3-5 Gy, e.g. 3 Gy) on day -2 prior to transplantation, on day -1 the subject is infused (e.g. IV) with a first dose of megadose T cell depleted immature hematopoietic cells comprising CD34 + selected cells (e.g.
  • the subject is infused (e.g. IV) with a second dose of megadose T cell depleted immature hematopoietic cells comprising CD3/CD19-depleted cells (e.g. cells collected after mobilization of a donor, depleted of CD3/CD19 expressing cells and used as fresh cells as described herein), on days +3 and +4 the subject is administered (e.g. IV) with Cyclophosphamide (CY) each at a dose of e.g.
  • CY Cyclophosphamide
  • the subject is infused with the anti-viral veto cells of some embodiments ofo the invention at a dose of e.g. 2.5-10 x 10 6 CD8 + cells per kg ideal body weight.
  • the subject is administed with ATG (Thymoglobulin) in 3 consequetive doses (e.g. 2 mg per kg ideal body weight) on days -10 to -8 (prior to transplanation).
  • ATG Thimoglobulin
  • the subject is treated with a dose of Mesna (e.g. 10 mg/kg intravenous piggy back (IVPB) just prior to the first dose of cyclophosphamide.
  • a dose of Mesna e.g. 10 mg/kg intravenous piggy back (IVPB) just prior to the first dose of cyclophosphamide.
  • IVPB intravenous piggy back
  • this is repeated every 4 hours for a total of 10 doses.
  • the subject is treated with ondansetron (or another anti-emetic) prior to each dose of Cyclophosphamide (CY).
  • ondansetron or another anti-emetic
  • premedication for the veto cells does not include corticosteroids.
  • the pre- transplant conditioning regimen comprises rituximab (e.g. at a dose of about 375 mg/m2, e.g. on day -10 prior to transplantation).
  • the number of administrations and the therapeutically effective amount of the pre- transplant and post-transplant immunosuppressive drugs and/or immunosuppressive irradiation may be adjusted as needed taking into account the type of transplantation and the subject's response to the regimen. Determination of the number of administrations and the therapeutically effective amount is well within the capability of those skilled in the art, especially in light of the detailed disclosure provided herein.
  • the method of the present invention utilizes the novel Tcm cells (as described in detail hereinabove).
  • the non-GVHD inducing cells comprising a Tcm phenotype i.e. veto cells
  • the non-GVHD inducing cells comprising a Tcm phenotype are used per se for reduction of graft rejection and or for reduction of GVHD of transplanted cells, tissues or organs (e.g. transplanted from the same donor).
  • the non-GVHD inducing cells comprising a Tcm phenotype i.e. veto cells
  • veto cells are for administration either concomitantly with, prior to, or following the transplantation of the cell or tissue transplant.
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration either concomitantly with, prior to, or following the transplantation of immature hematopoietic cells (e.g. T cell depleted immature hematopoietic cells).
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration following the non-syngeneic cell or tissue transplant.
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration following the immature hematopoietic cells (e.g. T cell depleted immature hematopoietic cells).
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 21 following the non-syngeneic cell or tissue transplant (e.g. T cell depleted immature hematopoietic cells).
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 21 following the immature hematopoietic cells (e.g. T cell depleted immature hematopoietic cells).
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 6-9 following the non-syngeneic cell or tissue transplant (e.g. immature hematopoietic cells e.g. T cell depleted immature hematopoietic cells).
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 9 following the non-syngeneic cell or tissue transplant.
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 8 following the non-syngeneic cell or tissue transplant.
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 7 following the non-syngeneic cell or tissue transplant.
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 6 following the non-syngeneic cell or tissue transplant.
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 9 following the immature hematopoietic cells (e.g. T cell depleted immature hematopoietic cells).
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 8 following the immature hematopoietic cells (e.g. T cell depleted immature hematopoietic cells).
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 7 following the immature hematopoietic cells (e.g. T cell depleted immature hematopoietic cells).
  • the non-GVHD inducing cells comprising a Tcm phenotype are for administration on day 6 following the immature hematopoietic cells (e.g. T cell depleted immature hematopoietic cells).
  • the veto cells when veto cells are used as adjuvant treatment for transplantation of cells or tissues obtained from a solid organ or tissue (e.g. kidney, pulmonary cells, liver cells, pancreatic cells, cardiac cells, etc. as discussed above), the veto cells may be administered concomitantly with, prior to, or following the transplantation of the cell or tissue transplant.
  • the veto cells, the immature hematopoietic cells and the cells or tissues can be administered in any order (e.g. kidney, immature hematopoietic cells and veto cells; immature hematopoietic cells, kidney and veto cells; etc).
  • immature hematopoietic cells e.g. T cell depleted immature hematopoietic cells
  • cells or tissues obtained from a solid organ or tissue e.g. kidney, pulmonary cells, liver cells, pancreatic cells, cardiac cells, etc. as discussed above
  • the veto cells and the immature hematopoietic cells are administered and the cell or tissue graft is transplanted only following establishment of chimerism (e.g. immature hematopoietic cells, veto cells an kidney).
  • the immature hematopoietic cells may be administered in a single administration, e.g. comprising CD3 + /CD19 + -depleted CD34+ cells (e.g. when using cryopreserved cells which are thawed on the day of transplantation).
  • the immature hematopoietic cells may be administered in two or more administrations, e.g. a first administration comprising CD34 + selected cells and a second administration comprising CD3 + /CD19 + -depleted cells (e.g. when using fresh cells, i.e. cells which are collected and used within about 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 days, e.g. within about 1 day). It will be appreciated that these can be administered concomitantly or subsequent to each other (e.g. on the same day or within 1, 2, 3, 4, 5 days of each other).
  • the non-GVHD inducing cells comprising a Tcm phenotype of some embodiments of the invention may be administered via any method known in the art for cell transplantation, such as but not limited to, cell infusion (e.g. intravenous) or via an intraperitoneal rout.
  • cell infusion e.g. intravenous
  • intraperitoneal rout e.g. intraperitoneal rout
  • the non-GVHD inducing cells comprising a Tcm phenotype are used as fresh cells.
  • the non-GVHD inducing cells comprising a Tcm phenotype are cryopreserved.
  • non-GVHD inducing cells comprising a Tcm phenotype of some embodiments of the invention can be administered to an organism per se, or in a pharmaceutical composition where it is mixed with suitable carriers or excipients.
  • the cell or tissue transplant e.g. immature hematopoietic cells (e.g. T cell depleted immature hematopoietic cells) of some embodiments of the invention can be administered to an organism per se, or in a pharmaceutical composition where it is mixed with suitable carriers or excipients.
  • immature hematopoietic cells e.g. T cell depleted immature hematopoietic cells
  • suitable carriers or excipients e.g. T cell depleted immature hematopoietic cells
  • a "pharmaceutical composition” refers to a preparation of one or more of the active ingredients described herein with other chemical components such as physiologically suitable carriers and excipients.
  • the purpose of a pharmaceutical composition is to facilitate administration of a compound to an organism.
  • active ingredient refers to the Tcm cells accountable for the biological effect.
  • physiologically acceptable carrier and “pharmaceutically acceptable carrier” which may be interchangeably used refer to a carrier or a diluent that does not cause significant irritation to an organism and does not abrogate the biological activity and properties of the administered compound.
  • An adjuvant is included under these phrases.
  • excipient refers to an inert substance added to a pharmaceutical composition to further facilitate administration of an active ingredient.
  • excipients include calcium carbonate, calcium phosphate, various sugars and types of starch, cellulose derivatives, gelatin, vegetable oils and polyethylene glycols.
  • Suitable routes of administration may, for example, include oral, rectal, transmucosal, especially transnasal, intestinal or parenteral delivery, including intramuscular, subcutaneous and intramedullary injections as well as intrathecal, direct intraventricular, intracardiac, e.g., into the right or left ventricular cavity, into the common coronary artery, intravenous, intraperitoneal, intranasal, or intraocular injections.
  • neurosurgical strategies e.g., intracerebral injection or intracerebroventricular infusion
  • molecular manipulation of the agent e.g., production of a chimeric fusion protein that comprises a transport peptide that has an affinity for an endothelial cell surface molecule in combination with an agent that is itself incapable of crossing the BBB
  • pharmacological strategies designed to increase the lipid solubility of an agent (e.g., conjugation of water-soluble agents to lipid or cholesterol carriers)
  • the transitory disruption of the integrity of the BBB by hyperosmotic disruption resulting from the infusion of a mannitol solution into the carotid artery or the use of a biologically active agent such as an angiotensin peptide).
  • each of these strategies has limitations, such as the inherent risks associated with an invasive surgical procedure, a size limitation imposed by a limitation inherent in the endogenous transport systems, potentially undesirable biological side effects associated with the systemic administration of a chimeric molecule comprised of a carrier motif that could be active outside of the CNS, and the possible risk of brain damage within regions of the brain where the BBB is disrupted, which renders it a suboptimal delivery method.
  • compositions of some embodiments of the invention may be manufactured by processes well known in the art, e.g., by means of conventional mixing, dissolving, granulating, dragee-making, levigating, emulsifying, encapsulating, entrapping or lyophilizing processes.
  • compositions for use in accordance with some embodiments of the invention thus may be formulated in conventional manner using one or more physiologically acceptable carriers comprising excipients and auxiliaries, which facilitate processing of the active ingredients into preparations which, can be used pharmaceutically. Proper formulation is dependent upon the route of administration chosen.
  • the active ingredients of the pharmaceutical composition may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hank’s solution, Ringer’s solution, or physiological salt buffer.
  • physiologically compatible buffers such as Hank’s solution, Ringer’s solution, or physiological salt buffer.
  • penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art.
  • the pharmaceutical composition can be formulated readily by combining the active compounds with pharmaceutically acceptable carriers well known in the art.
  • Such carriers enable the pharmaceutical composition to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions, and the like, for oral ingestion by a patient.
  • Pharmacological preparations for oral use can be made using a solid excipient, optionally grinding the resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries if desired, to obtain tablets or dragee cores.
  • Suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethyl-cellulose, sodium carbomethylcellulose; and/or physiologically acceptable polymers such as polyvinylpyrrolidone (PVP).
  • disintegrating agents may be added, such as cross-linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate.
  • Dragee cores are provided with suitable coatings.
  • suitable coatings For this purpose, concentrated sugar solutions may be used which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, carbopol gel, polyethylene glycol, titanium dioxide, lacquer solutions and suitable organic solvents or solvent mixtures.
  • Dyestuffs or pigments may be added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses.
  • compositions which can be used orally include push-fit capsules made of gelatin as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol.
  • the push-fit capsules may contain the active ingredients in admixture with filler such as lactose, binders such as starches, lubricants such as talc or magnesium stearate and, optionally, stabilizers.
  • the active ingredients may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols.
  • stabilizers may be added. All formulations for oral administration should be in dosages suitable for the chosen route of administration.
  • compositions may take the form of tablets or lozenges formulated in conventional manner.
  • the active ingredients for use according to some embodiments of the invention are conveniently delivered in the form of an aerosol spray presentation from a pressurized pack or a nebulizer with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichloro-tetrafluoroethane or carbon dioxide.
  • a suitable propellant e.g., dichlorodifluoromethane, trichlorofluoromethane, dichloro-tetrafluoroethane or carbon dioxide.
  • the dosage unit may be determined by providing a valve to deliver a metered amount.
  • Capsules and cartridges of, e.g., gelatin for use in a dispenser may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.
  • compositions described herein may be formulated for parenteral administration, e.g., by bolus injection or continuous infusion.
  • Formulations for injection may be presented in unit dosage form, e.g., in ampoules or in multidose containers with optionally, an added preservative.
  • the compositions may be suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents.
  • compositions for parenteral administration include aqueous solutions of the active preparation in water-soluble form.
  • suspensions of the active ingredients may be prepared as appropriate oily or water based injection suspensions.
  • Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acids esters such as ethyl oleate, triglycerides or liposomes.
  • Aqueous injection suspensions may contain substances, which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol or dextran.
  • the suspension may also contain suitable stabilizers or agents which increase the solubility of the active ingredients to allow for the preparation of highly concentrated solutions.
  • the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., sterile, pyrogen-free water based solution, before use.
  • compositions of some embodiments of the invention may also be formulated in rectal compositions such as suppositories or retention enemas, using, e.g., conventional suppository bases such as cocoa butter or other glycerides.
  • the non-GVHD inducing cells comprising a Tcm phenotype are formulated for administration as fresh cells.
  • the non-GVHD inducing cells comprising a Tcm phenotype are formulated for administration as cryopreserved cells.
  • compositions suitable for use in context of some embodiments of the invention include compositions wherein the active ingredients are contained in an amount effective to achieve the intended purpose. More specifically, a therapeutically effective amount means an amount of active ingredients (non-GVHD inducing Tcm cells) efficient for tolerization (i.e. veto effect), anti-disease effect, anti-tumor effect and/or immune reconstitution without inducing GVHD. Since the Tcm cells of the present invention home to the lymph nodes following transplantation, lower amounts of cells (compared to the dose of cells previously used, see for example WO 2001/049243) may be needed to achieve the beneficial effect/s of the cells (e.g. tolerization, anti-disease, anti-tumor effect and/or immune reconstitution).
  • the therapeutically effective amount or dose can be estimated initially from in vitro and cell culture assays.
  • a dose can be formulated in animal models to achieve a desired concentration or titer. Such information can be used to more accurately determine useful doses in humans.
  • the number of Tcm cells infused to a subject should be more than 1 x 10 6 cells per kg ideal body weight.
  • the number of Tcm cells infused to a subject should typically be in the range of 0.01 x 10 6 to 20 x 10 6 cells per kg ideal body weight, 0.01 x 10 6 to 0.5 x 10 6 cells per kg ideal body weight, 0.01 x 10 6 to 1 x 10 6 cells per kg ideal body weight, 0.01 x 10 6 to 5 x 10 6 cells per kg ideal body weight, 0.1 x 10 6 to 0.5 x 10 6 cells per kg ideal body weight, 0.1 x 10 6 to 1 x 10 6 cells per kg ideal body weight, 0.1 x 10 6 to 5 x 10 6 cells per kg ideal body weight, 0.5 x 10 6 to 1 x 10 6 cells per kg ideal body weight, 0.5 x 10 6 to 1 x 10 6 cells per kg ideal body weight, 0.5 x 10 6 to 1 x 10 6 cells per kg ideal body weight, 0.5 x 10 6 to 1 x 10 6 cells per kg ideal body weight, 0.5 x 10 6 to 5 x 10 6 cells per kg ideal body weight, 1 x 10
  • the dose of Tcm cells infused to a subject is about 2.5 x 10 6 CD8 + cells per kg ideal body weight.
  • the dose of Tcm cells infused to a subject is about 5 x 10 6 CD8 + cells per kg ideal body weight.
  • the dose of Tcm cells infused to a subject is about 10 x 10 6 CD8 + cells per kg ideal body weight.
  • implant body weight refers to the measurement used clinically to adjust drug dosing, help estimate renal function and the pharmacokinetics (such as in obese patients).
  • IBW 50 kg + 2.3 kg for each inch over 5 feet.
  • Toxicity and therapeutic efficacy of the active ingredients described herein can be determined by standard pharmaceutical procedures in vitro, in cell cultures or experimental animals.
  • the data obtained from these in vitro and cell culture assays and animal studies can be used in formulating a range of dosage for use in human.
  • the dosage may vary depending upon the dosage form employed and the route of administration utilized.
  • the exact formulation, route of administration and dosage can be chosen by the individual physician in view of the patient's condition. (See e.g., Fingl, et al., 1975, in "The Pharmacological Basis of Therapeutics", Ch. 1 p.1).
  • Dosage amount and interval may be adjusted individually to provide ample levels of the active ingredient which are sufficient to induce or suppress the biological effect (minimal effective concentration, MEC).
  • MEC minimum effective concentration
  • the MEC will vary for each preparation, but can be estimated from in vitro data. Dosages necessary to achieve the MEC will depend on individual characteristics and route of administration. Detection assays can be used to determine plasma concentrations. Depending on the severity and responsiveness of the condition to be treated, dosing can be of a single or a plurality of administrations, with course of treatment lasting from several days to several weeks or until cure is effected or diminution of the disease state is achieved .
  • dosing can be of a single or a plurality of administrations, with course of treatment lasting from several days to several weeks or until cure is effected or diminution of the disease state is achieved .
  • compositions to be administered will, of course, be dependent on the subject being treated, the severity of the affliction, the manner of administration, the judgment of the prescribing physician, etc.
  • compositions of some embodiments of the invention may, if desired, be presented in a pack or dispenser device, such as an FDA approved kit, which may contain one or more unit dosage forms containing the active ingredient.
  • the pack may, for example, comprise metal or plastic foil, such as a blister pack.
  • the pack or dispenser device may be accompanied by instructions for administration.
  • the pack or dispenser may also be accommodated by a notice associated with the container in a form prescribed by a governmental agency regulating the manufacture, use or sale of pharmaceuticals, which notice is reflective of approval by the agency of the form of the compositions or human or veterinary administration. Such notice, for example, may be of labeling approved by the U.S. Food and Drug Administration for prescription drugs or of an approved product insert.
  • Compositions comprising a preparation of the invention formulated in a compatible pharmaceutical carrier may also be prepared, placed in an appropriate container, and labeled for treatment of an indicated condition, as is further detailed above.
  • the non-GVHD inducing cells comprising a Tcm phenotype of some embodiments of the invention can be manufactured for an “off-the-shelf’ product for therapy (e.g. as veto cells for use in therapy as discussed above).
  • the subject is not treated chronically (e.g. for a prolonged period of time, e.g. for more than 8, 9, 10, 12, 14, 21, 25, 30, 45, 60 or 90 days e.g. 10-21 days, e.g. 10 or 14 days) with GVHD prophylaxis post-transplant (e.g. with corticosteroids and/or with immunosuppressive agents).
  • chronically e.g. for a prolonged period of time, e.g. for more than 8, 9, 10, 12, 14, 21, 25, 30, 45, 60 or 90 days e.g. 10-21 days, e.g. 10 or 14 days
  • GVHD prophylaxis post-transplant e.g. with corticosteroids and/or with immunosuppressive agents.
  • the subject in case of relapse after hematopoietic stem cell transplantation, the subject may be further treated by donor lymphocyte infusions (DLIs).
  • DLIs donor lymphocyte infusions
  • the subject may be administered with graded doses of T-cells as previously described by Dazzi et al [Dazzi, Szydlo et al., Blood, (2000) 96: 2712-6] fully incorporated herein by reference.
  • compositions, method or structure may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure.
  • a compound or “at least one compound” may include a plurality of compounds, including mixtures thereof.
  • range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range.
  • a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range.
  • the phrases “ranging/ranges between” a first indicate number and a second indicate number and “ranging/ranges from” a first indicate number “to” a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween.
  • method refers to manners, means, techniques and procedures for accomplishing a given task including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the chemical, pharmacological, biological, biochemical and medical arts.
  • FL follicular lymphoma
  • MCL mantle cell lymphoma
  • CLL chronic lymphocytic leukemia
  • MM multiple myeloma
  • NHL Hodgkin’s lymphoma
  • NHL non-hodgkin’s lymphoma
  • NDL non-hodgkin’s lymphoma
  • CML chronic myeloid leukemia
  • MPD myeloproliferative syndromes
  • AML acute myeloid leukemia
  • ALL acute lymphoid leukemia
  • Pulmonary function test (PFT) demonstrating an adjusted diffusion capacity of least
  • MNC mononuclear cells
  • PBPCs mobilized peripheral blood progenitor cells
  • the collection of unprimed (i.e. non-mobilized) peripheral blood mononuclear cells for production of the anti-viral central memory CD8 + veto T cells may be done at any time, but generally are done about 7 days before the planned transplant date (i.e. Day 0, i.e. DO).
  • the veto cells are either used fresh at the end of their manufacturing and are to be infused on D+7, or are cryopreserved at the end of their manufacturing.
  • the collection of the mobilized PBPCs using G-CSF and plerixafor, if clinically necessary, for the megadose transplant can be completed 7 days or more before collection of the PBMCs for production of the of the anti- viral central memory CD8 + veto cells.
  • fresh CD34 + stem cells can be used wherein collection of the mobilized stem cells can be performed on three consecutive days (day -2, day -1 and day 0) while collection of PBMC for veto cells can begin on day -8.
  • An optimal PBMC cell dose goal of > 10 x 10 6 CD34 + cells per kg ideal body weight is procured; a minimum of 5 x 10 6 CD34 + cells per kg ideal body weight is required which includes an unfractionated aliquot of PBPCs containing approximately 1 x 10 6 CD34 + cells per kg ideal body weight to be cryopreserved as a backup in case of inadequate veto cell production or graft failure post- transplant or to be available for treatment of graft-failure. If the donor does not tolerate the procedure, or an adequate cell dose cannot be collected, an alternative donor may be used if available.
  • the mobilized PBPCs are generally collected in 3 days.
  • the first 2 days collections are pooled and CD34 + cells selected using the CliniMacs device (Miltenyi), the cells can be used fresh (along with the cells of the third collection) or may be cryopreserved.
  • the third day's collection are depleted of CD3 + /CD19 + cells, the cells can be used fresh (along with the cells of the previous 2 days collections) or may be cryopreserved.
  • the maximum T-cell dose in the entire T-cell depleted stem cell transplant is 2 x 10 5 CD3+ cells/kg ideal recipient body weight.
  • the back-up fraction of unmanipulatd PBPCs are removed from the first day’s collection before the CD34 + cell selection procedure is performed on the remaining PBPC product.
  • Example 2 A detailed protocol for production of veto cells is provided in Example 2, hereinbelow. This section provides a brief description of some embodiments of the invention.
  • Step 1 Preparation of Stimulators (Day -8 to day -5):
  • Day -8 Approximately 1 x 10 10 mononuclear cells are collected by leukapheresis and kept overnight.
  • Day-7 The cells are processed by Ficol separation and thereafter half of the mononuclear cells are used for monocyte isolation by CD14 magnetic beads (Miltenyi magnetic beads sorting system. Thereafter, the CD14 + monocytes are differentiated to immature DC with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin 4 (IL-4).
  • CD14 magnetic beads Miltenyi magnetic beads sorting system. Thereafter, the CD14 + monocytes are differentiated to immature DC with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin 4 (IL-4).
  • GM-CSF granulocyte macrophage-colony stimulating factor
  • IL-4 interleukin 4
  • CD14- (i.e. CD14 neg) cells are combined with half of the initial mononuclear cells obtained after ficol isolation and stored overnight in 37 °C 5% O2/CO2 in the presence of IL-7. These cells are used for the enrichment of CD8 + memory T cells on the next day.
  • Day -6 DC Maturation by addition of cytokine-cocktail comprising lipopolysaccharide (EPS), Interferon gamma (IFNg), GM-CSF, and IL-4 for 16 hours of incubation.
  • EPS lipopolysaccharide
  • IFNg Interferon gamma
  • GM-CSF GM-CSF
  • IL-4 IL-4
  • Step 2 Preparation of responders and co-culture with viral loaded mDC ( Day -6 to Day -5):
  • Day -6 Donor CD14- cells combined with half of the mononuclear cells that were incubated overnight with IL-7, are depleted of CD4 + , CD56 + , and CD45RA + cells by magnetic beads and stored for an additional night in 37 °C 5% 02/C02 in the presence of IL-7.
  • Days -5 to -2 Initiation of Tcm phenotype with IL-21 only.
  • Days -2 to +7 Expansions of Tcm cultures by splitting according to glucose level consumption and addition of medium supplemented with the following cytokines: IL-7, IL-15 and IL-21 depending on the observed expansion in culture.
  • the conditioning regimen from D-10 to D-2 includes Anti-thymocyte globulin (ATG) (3 doses from day -10 to day -8), Fludarabine (Flu) (4 doses from day-7 to day -4) and 3 Gy total body irradiation (TBI) (once on day-2).
  • ATG Anti-thymocyte globulin
  • Fludarabine Fludarabine
  • TBI 3 Gy total body irradiation
  • the T-cell depleted stem cell graft is infused on day -1 and day 0.
  • D+3 and +4 post-transplant i.e. days 3 and 4 after the transplant
  • the patient receives cyclophosphamide (CY) followed by the infusion of the veto cells on D+7 post-transplant (i.e. on day 7 after transplant).
  • Patients with B-cell malignancies may receive rituximab 375 mg/m 2 on day -11.
  • Premedication for the Veto cells does not include corticosteroids.
  • the patient receives Anti-thymocyte globulin (ATG), Fludarabine (Fu) and low dose total body irradiation (TBI) as the preparative regimen.
  • ATG Anti-thymocyte globulin
  • Fu Fludarabine
  • TBI low dose total body irradiation
  • D+3 and +4 after transplant i.e. days 3 to 4 after the transplant
  • the patient receives cyclophosphamide (CY) followed by the infusion of the veto cells on day 7 post-transplant (D+7 after transplant).
  • Patients with B-cell malignancies may receive rituximab 375 mg/m 2 on day -10.
  • Day and Treatment :
  • IVPB intravenous piggy back
  • Premedication for the Veto cells does not include corticosteroids.
  • Anti-viral veto cell dose levels - CD8 + Cell dose escalation Dose level 1: 2.5 x 10 6 CD8 + cells per kg ideal body weight
  • Dose level 2 5.0 x 10 6 CD8 + cells per kg ideal body weight
  • Dose level 3 10 x 10 6 CD8 + cells per kg ideal body weight
  • No additional post-transplant immunosuppressive therapy is administered as GVHD prophylaxis.
  • Dose level one comprises 2.5 x 10 6 cells per kg ideal body weight. Of note, a lower dose is not typically considered acceptable.
  • the target dose is 5 x 10 6 cells per kg ideal body weight, however, a dose of 2.6 to 5 x 10 6 cells per kg ideal body weight is considered adequate and considered dose level 2.
  • the target dose is 10 x 10 6 cells per kg ideal body weight, however, a dose of 5.1 to 10 x 10 6 cells per kg ideal body weight is considered adequate, and considered dose level
  • Standard work up for transplant as well as disease assessment is done prior to study entry as part of diagnostic or routine pre-transplant evaluation.
  • the following tests are standard of care pre- transplant tests and not protocol specific. The results are used to determine transplant eligibility and are not repeated prior to the beginning of treatment. If the treatment is delayed for more than 30 days after consenting, the PI or designee should determine which, if any, tests need to be repeated as clinically indicated.
  • Bilirubin serum creatinine and creatinine clearance, ALT, albumin, electrolytes, LDH, alkaline phosphatase
  • Infectious diseases panel hepatitis serology (B, C), HIV, HTLV, I/II, CMV, TPHA screen), toxoplasma serology, Strongyloides serology (if indicated), tuberculosis, IRGA (if indicated)
  • DSA Serum for donor-specific anti-HLA antibodies
  • the active treatment period for patients treated in this study is from the beginning of the preparative regimen (Day -9) through Day +42 post-transplant. After that, patients have follow-up as clinically indicated through D+100. Thereafter, disease relapse, infections, acute and chronic GVHD and survival data are collected according to the standard follow up of stem cell transplant recipients. After one year, patients are removed from the study. Acute GVHD and Chronic GVHD are scored according to NIH Consensus Criteria.
  • Standard Post Evaluations are per SOC post allogeneic transplant. Bone marrow aspiration and peripheral blood - to evaluate treatment response, engraftment, chimerism and immune reconstitution - are performed monthly for 3 months and as clinically indicated.
  • Immune reconstitution is assessed by flow cytometry immunophenotype panel and antiviral responses by tetramer analysis. Immune tolerance test is performed approximately 3 months post- transplant. Missed samples for correlative studies are not constitute protocol deviations.
  • GVHD chronic GVHD
  • NIH Consensus Criteria Jagasia MH, et al. National Institutes of Health Consensus Development Project on Criteria for Clinical Trials in Chronic Graft-versus-Host Disease: I. The 2014 Diagnosis and Staging Working Group report. Biol Blood Marrow Transplant. (2015) 21:389-401 e381].
  • the cells are infused if the dose is > 2.5 x 10 6 CD8 + cells per kg ideal body weight (dose level 1) In this case, the patients are evaluated in their assigned dose level by the intention to treat principle.
  • the veto cells dose is less than 2.5 x 10 6 CD8 + cells per kg ideal body weight, or if the veto cells do not meet release criteria, these cells are not infused. Instead, the backup PBPCs plus the T cell depleted PBPCs are infused to reconstitute a "standard PBPC transplant," and the patient receives a standard of care preparative regimen, with tacrolimus and mycophenolate as standard GVHD prophylaxis.
  • GVHD GVHD
  • patients who have achieved engraftment and have > 5 % myeloid donor cells and who have progressive disease at any time or persistent disease at > 3 months post- transplant may receive donor lymphocyte infusion as standard of care or alternative therapy.
  • Engraftment is defined as detection of donor cells by chimerism analysis and recovery of an absolute neutrophil count, ANC > 0.5 x 10 9 /L.
  • ANC donor cell engraftment and hematopoietic recovery
  • Acute GVHD a) Grade I - monitor without medication (Rx) for 1 week or topical steroids only. b) If grade II- IV occurs, treat with methylprednisolone 1-2 mg/kg ideal body weight/day. c) If, after addition of methylprednisolone, grade 3 II persists for 48 hrs or progresses, tacrolimus or other immunosuppressive medications may be added as clinically indicated or on active GVHD treatment protocols. Patients with grade 3-4 steroid resistant acute GVHD are considered treatment failures and may receive other immunosuppressive treatments as clinically indicated.
  • the primary scientific goal of the trial is to determine an optimal veto cell dose among the three levels 2.5 x 10 6 , 5 x 10 6 , and 10 7 CD8 + cells per kilogram ideal body weight, hereafter referred to and coded numerically in the dose-outcome model as doses 1, 2, 3 (see Table IB, below).
  • Dose-finding are done using the sequentially adaptive phase I-II EffTox trade-off- based design as previously discussed [Thall PF and Cook JD. Dose-finding based on efficacy- toxicity trade-offs. Biometrics (2004) 60:684-693; Thall PF et al. Using effective sample size for prior calibration in Bayesian phase I-II dose-finding. Clinical Trials. (2014) 11:657-666].
  • Toxicity is defined as a grade 3 or 4 GVHD, or death from any cause, within 42 days of veto cell infusion; and (2) Efficacy is defined as the patient being alive and engrafted at day 42 post veto cell infusion. A patient who is enrolled in the trial but, for any reason, does not receive veto cells on day 7 post allosct are replaced in the sample.
  • Dose Acceptability For the two EffTox dose acceptability rules, the upper limit on the probability of Toxicity is .20, the minimum probability of Efficacy .80, and posterior probability decision cut-offs .10 are used or both acceptability criteria. If the lowest dose level is found to be unacceptable in terms of either high Toxicity or low Efficacy, the trial are terminated and no veto cell dose level are selected.
  • Prior The prior on the model parameters was computed using the EffTox program version 4.0.12, from the elicited mean marginal outcome probabilities given on Table IB, with hyper- variances calibrated to obtain prior effective sample size 1.
  • the EffTox design are implemented using the MD Anderson Cancer Center (MDACC) Department of Biostatistics Clinical Trial Conduct website www(dot)biostatistics(dot)mdanderson (dot)org/ClinicalTrialConduct/.
  • MDACC MD Anderson Cancer Center
  • the study biostatistician Peter Thall or a designated Statistical Analyst in the Biostatistics Department should be consulted if necessary during trial conduct.
  • Secondary outcomes include infection, progression-free survival (PFS) time, overall survival (OS) time, cytomegalovirus, and immune reconstitution, with a 1-year follow up period for each patient.
  • Unadjusted distributions of time-to-event outcomes are estimated using the method of Kaplan and Meier [Kaplan, EL and Meier, P: Nonparametric estimator from incomplete observations. J. American Statistical Association (1958) 53:457-481] and their relationship to prognostic covariates and veto cell dose level are evaluated by Bayesian piecewise exponential survival regression [Ibrahim JG, Chen M-H, Sinha D. Bayesian Survival Analysis. Springer, NY (2001)].
  • An adverse event is the appearance or worsening of any undesirable sign, symptom, or medical condition occurring after starting the study drug even if the event is not considered to be related to study drug.
  • Medical conditions/diseases present before starting study drug are only considered adverse events if they worsen after starting study drug.
  • Abnormal laboratory values or test results constitute adverse events only if they induce clinical signs or symptoms, are considered clinically significant, or require therapy.
  • the Investigator or physician designee is responsible for verifying and providing source documentation for all adverse events and assigning the attribution for each event for all subjects enrolled on the trial.
  • Redcap is used as the electronic case report form for this protocol.
  • Grade 1 Mild: discomfort present with no disruption of daily activity, no treatment required beyond prophylaxis.
  • Grade 2 Moderate: discomfort present with some disruption of daily activity, require treatment.
  • Grade 3 Severe: discomfort that interrupts normal daily activity, not responding to first line treatment.
  • Grade 4 Life Threatening: discomfort that represents immediate risk of death
  • abnormal laboratory findings considered associated to the original disease as well as isolated changes in laboratory parameters such as electrolyte magnesium and metabolic imbalances, uric acid changes, elevations of GPT, GOT, LDH and alkaline phosphatase are not considered adverse events and are not be collected in the database.
  • a life-threatening adverse drug experience any adverse experience that places the patient, in the view of the initial reporter, at immediate risk of death from the adverse experience as it occurred. It does not include an adverse experience that, had it occurred in a more severe form, might have caused death.
  • Important medical events that may not result in death, be life-threatening, or require hospitalization may be considered a serious adverse drug experience when, based upon appropriate medical judgment, they may jeopardize the patient or subject and may require medical or surgical intervention to prevent one of the outcomes listed in this definition.
  • Examples of such medical events include allergic bronchospasm requiring intensive treatment in an emergency room or at home, blood dyscrasias or convulsions that do not result in inpatient hospitalization, or the development of drug dependency or drug abuse (21 CFR 312.32).
  • Important medical events as defined above may also be considered serious adverse events. Any important medical event can and should be reported as an SAE if deemed appropriate by the Principal Investigator or the IND Sponsor, IND Office.
  • Serious adverse events are captured from the time of the first protocol- specific intervention, until 30 days after infusion of anti-Veto cells, unless the participant withdraws consent. Serious adverse events must be followed until clinical recovery is complete and laboratory tests have returned to baseline, progression of the event has stabilized, or there has been acceptable resolution of the event.
  • Doses of up to 1.5 gm/m 2 /d x 4 may be used in conjunction with bone marrow or peripheral blood progenitor cell transplant.
  • Cyclophosphamide is considered a classical bifunctional alkylating agent, with the predominant alkylation reaction occurring at the 7 nitrogen of guanine. Cyclophosphamide must be activated by liver microsomal enzymes in order to damage the DNA molecule. Although active throughout the cell cycle, the agent is most active during the S phase.
  • Cyclophosphamide can be given orally or intravenously, although oral absorption is incomplete (30-60 % of a dose is recoverable in the stool). Maximum plasma levels are achieved within one hour from an oral dose. Plasma T-l/2 is 4-6.5 hours. Approximately 60 % of an intravenous dose is recovered in the urine within 24 hours, requiring dosage adjustments in patients with renal insufficiency. The drug is activated and subsequently deactivated by liver microsomal enzymes.
  • Each vial contains 50 mg lyophilized drug, to be reconstituted with 2 ml sterile water to a solution that is 25 mg/ml for IV administration.
  • Solution Preparation mix each vial with 2 ml sterile pyrogen-free water to a clear solution, which is 25 mg/ml for IV administration only. Reconstituted solution should be used within 8 hours.
  • Anti-thymocyte globulin tATG Anti-thymocyte globulin tATG
  • ATG is a rabbit anti-thymocyte globulin. It is a purified, sterile, primarily monomeric IgG fraction of hyperimmune serum of selective immunosuppressant. It is believed to act by modifying the number and function of lymphocytes.
  • ATG is diluted in 0.9 % NaCl.
  • ATG is given intravenously as a 4 hour infusion into a central vein. It is a colorless to slightly opalescent protein solution.
  • ATG should not be diluted in dextrose or low salt fluids, as this may cause precipitation. Infusion with acidic solution may cause physical instabilities. Although no further incompatibilities or drug interactions are known, it is recommended to administer ATG alone.
  • Solution for infusion contains 1 mg of ATG/2 ml. It should be stored in a refrigerator at 2 °C to 8 °C (do not freeze). Diluted ATG is stable for 24 hours.
  • ATG may cause cytopenia of any cell line, chills, fever, or edema.
  • a serum- sickness like syndrome may also develop.
  • Adverse reactions including rash, pruritus, urticaria, wheal and flare reactions, bronchospasm bleeding and anaphylactic shock have been reported.
  • Resuscitation equipment should be available during administration. After an anaphylactic reaction, infusion should not be resumed.
  • IV mesna should continue 12-24 hr after the completion of ifosfamide or cyclophosphamide.
  • Contraindications hypersensitivity to mesna/other thiol compounds.
  • This protocol requires the collection of two separate stem cells preparations.
  • One type of stem cell preparation is from a mobilized haploidentical donor, part of which is selected for CD34 + cells, and the other part depleted of CD3 + /CD19 + cells using the Miltenyi CliniMACS ® device and cryopreserved.
  • the second type of preparation is an allogeneic non-mobilized peripheral blood mononuclear (PBMC) cells for the production of the anti-viral central memory CD8 + veto T cells.
  • the PBMC are used to generate dendritic cells and memory responder T cells (CD4- CD56- CD45RA- ), which are then co-cultured to stimulate production of anti-viral central memory CD8 + veto cells (Tcm).
  • Tcm cells are then expanded, harvested and infused. This preparation is used in the manufacture and infusion of Tcm cells, to achieve engraftment without GVHD after the infusion of T cell depleted megadose haploidentical donor from CD34 + enriched cells.
  • Cell preparations of some embodiments of the invention are processed in the Department of Stem Cell Transplantation and Cellular Therapy (SCTCT) Cell Therapy Laboratory (CTL) at MDACC.
  • SCTCT Stem Cell Transplantation and Cellular Therapy
  • CTL Cell Therapy Laboratory
  • This facility contains both a Cell Processing Laboratory for minimally manipulated cell preparations and Classified Suites (Class ISO 7) for more than minimally manipulated cell preparations.
  • the cell preparation of some embodiments of the invention is manufactured primarily in the Class ISO 7 suites.
  • the CTL also includes a Flow Cytometry and Quality Control Laboratories for the support of clinical trials, research, and development.
  • the SCTCT CTL is registered with the FDA (FEI #0001670014) and is accredited by the Foundation of Accreditation of Cellular Therapy (FACT), the College of American Pathologists (CAP), and holds a Clinical Laboratory Improvement Amendments (CLIA) Certificate for Accreditation issued by the Centers for Medicare and Medicaid Services (CMS).
  • FACT Foundation of Accreditation of Cellular Therapy
  • CAP College of American Pathologists
  • CCS Clinical Laboratory Improvement Amendments
  • PBMC Two separate cell type preparations are required for this protocol.
  • One preparation comprises an allogeneic non-mobilized peripheral blood mononuclear cells for the production of the anti-viral central memory CD8 + veto T cells (Tcm) while the other is from a mobilized haploidentical peripheral blood preparation. Both preparations are collected via leukapheresis.
  • the non-mobilized (unprimed) peripheral blood mononuclear cells for production of the Tcm cells are collected about 7 days before the planned transplant date (Day 0).
  • the target leukapheresis mononuclear cells yield is of 1 x 10 10 .
  • the Tcm cells are infused on D+7 after infusion of the CD34 + Enriched Preparation.
  • the mobilized peripheral blood cells are collected over 3 days.
  • the Day 1 and Day 2 collections are CD34 + selected and cryopreserved.
  • the third day’s collection is depleted of CD3 + /CD19 + cells and cryopreserved. Multiple collections can be pooled prior to the enrichment procedure.
  • a back up fraction of unmodified PBMC containing 2 x 10 6 CD34 + cells per kg ideal body weight is taken from the day 1 collection and cryopreserved.
  • MDACC donors are first assessed for suitability including eligibility screening and physical examination. Donors are then consented and scheduled for collection. Target cell numbers are protocol specific and multiple collections may be required. After the collection procedure is complete, cells are transported from the harvesting facility to the CTL Laboratory by CTL staff in plastic coolers to protect the cells from temperature fluctuations and physical damage. Preparations are then logged at the CTL.
  • PBMC Therapeutic Cells
  • Donors are considered ineligible if they have any of the following risk factors:
  • MDACC donor and patient medical histories are determined in the SCTCT Clinic by the Attending Physician or their designee(s).
  • Infectious disease testing includes Anti HIV-1 / HIV-2 (DHIV/1/2), Hepatitis C Virus Antibody (DHCVAB), Anti HTLV I / HTLVII (DHTLV I/II), Hepatitis B Antigen (DHBSAG), Hepatitis B Core Antibody (DHBCAB), West Nile Virus-NAT testing (WNV by Nucleic Acid Testing), Serology test for syphilis (Donor TP-HA or RPR), Anti CMV (Donor CMV Antibody), Chagas Disease, HIV, HCV antigen testing by Nucleic Acid Testing (NAT).
  • the NAT HIV/HCV testing is a combination testing. If result is reactive, discriminatory testing is performed.
  • All cell preparations according to one embodiment obtained from donors where eligibility has not been determined must be stored in quarantine until eligibility has been determined. Cell preparations in quarantine are clearly labeled to prevent release. If cell preparations must be released prior to eligibility determination then urgent medical need must be documented. These cell preparations must be labeled “NOT EVALUATED FOR INFECTIOUS SUBSTANCES” and “WARNING: Advise patient of communicable disease risks”. Release of Cell Preparations from Ineligible Donor The release of cell preparations of one embodiment from an ineligible donor requires the documentation of urgent medical need and the consent of the patient. Cell preparations are labeled with a “Biohazard” Label including “WARNING: Advise patient of communicable disease risks”.
  • the screening and test results must accompany the cell preparations and be provided to the infusing physician.
  • Infusion of Cell Preparations with Positive IDM Results The infusion of a cell preparation according to one embodiment with a positive IDM result requires the documentation of urgent medical need and the consent of the patient. Cell preparations are labeled with a “Biohazard” Label including “WARNING: Advise patient of communicable disease risks”. Screening and test results must accompany the cell preparation and be provided to the infusing physician. Prophylactic treatment and additional patient monitoring may be necessary.
  • PBMC Isolation and Thrombowash The non-mobilized leukapheresis cell preparation of one embodiment is diluted at 1:2 with Dulbecco’s Phosphate-Buffered Saline (DPBS) without Calcium and Magnesium supplemented with 0.5 % of Human Serum Albumin, 25 % (HSA). Samples for cell count, Trypan Blue (TB) viability and sterility are obtained prior to ficoll process. The MNC are isolated by ficoll density gradient separation.
  • DPBS Phosphate-Buffered Saline
  • HSA Human Serum Albumin
  • the MNC cell preparation of one embodiment is platelet washed (thrombowash) twice by manual centrifugation prior to CD14+ isolation and resuspended with Wash Buffer. Samples for cell counts and TB viability are removed for QC testing. Following the Post-Ficoll Thrombowash, the cells are divided into two equal fractions and diluted up to approximately 500 mL each. One half are processed for the Dendritic Cell (DC) isolation by CD14+ Selection and the other half (Fraction I) are kept overnight for the CD8+ Memory T Cell Enrichment process the following day.
  • DC Dendritic Cell
  • Fraction I the other half
  • Fraction I is centrifuged and resuspended at a concentration of 30 x 10 6 cells/ml in T Cell Growth Media (Click’s Media with advanced RPMI 1640 supplemented with 1:100 Glutamaxe and 5% Human AB Serum) along with IL-7 (30 IU/mL) Samples for sterility testing are removed. Fraction I is then plated onto tissue culture flasks and incubated overnight at 37 °C, 5 % CO2.
  • the DC isolation fraction is centrifuged and resuspended in 50 ml of Magnetic Bead Buffer (Dulbecco’s Phosphate-Buffered Saline (DPBS) without Calcium and Magnesium supplemented with 0.6% ACD-A and 0.5 % of 25 % HAS). Samples for cell count and TB viability are removed.
  • Magnetic Bead Buffer Dulbecco’s Phosphate-Buffered Saline (DPBS) without Calcium and Magnesium supplemented with 0.6% ACD-A and 0.5 % of 25 % HAS.
  • the DC Isolation MNC fraction is incubated with the CD14 Reagent (CD14 monoclonal antibodies conjugated to super-paramagnetic iron dextran particles).
  • the cells are then washed with Magnetic Beads buffer to remove excess reagent. Samples for cell count, TB viability and immunophenotyping are removed.
  • CD14 labeled cells are processed on the SuperMACSTM P using the XS Separation Column per established SOP.
  • the magnetically labeled cells (CD14 + ) are retained by the column and the CD14 negative cells are removed.
  • the CD14 + cells are then released from the column and collected.
  • the CD14 + fraction is washed and resuspended in DCs medium (CellGro/1% HSA), while CD14- fraction is washed and resuspended in T cell growth medium. Samples from each fraction are removed for cell count, TB viability and immunophenotyping.
  • the CD14 + enriched cell preparation of one embodiment is then continued to the DC Maturation step, while the CD14 negative cell concentration is adjusted at 30 x 10 6 cells/ml in T Cell Growth Media along with IL-7 (30 IU/mL). Samples for sterility testing are removed.
  • the CD14 Negative Fraction is then plated onto tissue culture flasks and incubated overnight at 37 °C, 5 % CO2. These cells are combined on the next day with Fraction I for the CD8 + Memory T Cell Enrichment process.
  • the CD14 + enriched cell preparation of one embodiment is resuspended at a cell concentration of 3 x 10 6 cells/ml in DC Medium (CellGro/1 % HSA) supplemented with IL-4 (1000 IU/mL) and GM-CSF (2000 IU/mL). Samples for sterility testing are removed. The cell suspension is then seeded in Cell Factory plates and incubated overnight for 16-24 hours in at 37 °C, 5 % CO 2 .
  • cytokines IL-4 (1000 IU/mL), GM-CSF (2000 IU/mL), LPS (40 ng/mL), and IFN-g (200 IU/mL) are added to the Cell Factories to induce maturation of the DCs.
  • the cells are then incubated with the cytokines at 37 °C, 5 % CO2 for 16 hours (+/- 2 hours).
  • the cells in Fraction I and in the CD14 Negative fraction are harvested and combined. Once combined (T Cell Isolation TNC Fraction), the cells are centrifuged and resuspended in CliniMACS ® /0.5 % HSA Buffer to a minimum of 1:2 ratio. Platelet depletion (thrombowash) is performed by centrifugation and resuspension of the cells pellet in CliniMACS ® /0.5 % HSA. A sample for cell count and TB viability is removed.
  • the post-platelet depleted cell preparation of one embodiment is incubated with IVIg for 10-15 minutes. After the initial incubation the CD4 + , CD56 + and CD45RA + reagents (CD4 + , CD56 + and CD45RA + antibodies conjugated to super-paramagnetic particles) are added to the cell preparation and incubated for 30 minutes on an orbital rotator. At the end of the incubation the cells are washed by centrifugation and the cell pellet resuspended in CliniMACS ® /0.5 % HSA buffer to remove excess reagent. Cell count and immunophenotyping samples are removed.
  • CD4 + , CD56 + and CD45RA + reagents CD4 + , CD56 + and CD45RA + antibodies conjugated to super-paramagnetic particles
  • the CD4 + /CD56 + /CD45RA + labeled cells are then processed on the CliniMACS ® using the depletion tubing set and the depletion program.
  • the magnetically labeled cells (CD4 + , CD56 + and CD45RA + ) are retained by the column and CD4, CD56 and CD45RA negative cells pass through the column and are collected as a CD4 CD56 CD45RA- Depleted Fraction (Fraction II).
  • Fraction II is washed and resuspended in T cell Growth Medium. Samples from each fraction are removed for cell count, TB viability and immunophenotyping. Once the volume and TNC of each fraction is determined, the positive fraction is discarded and the negative fraction (Fraction II) is further processed.
  • Fraction II cell concentration is adjusted at 2 x 10 6 cells/ml in T Cell Growth Media along with IL-7 (30 IU/mL). Samples for sterility testing are removed. Fraction II is seeded in G- Rex ® 100 and incubated at 37 °C, 5 % with CO2 for 24 hours (+/- 2 hours).
  • the maximum TNC to process with one reagent kit and tubing set is 200 x 10 6 TNC/mL unless higher number is approved by the Laboratory Director or designee.
  • the maximum load volume for the CliniMACS ® instrument is 300 ml.
  • the supernatant is removed and the Cell Factories are gently washed with warm Magnetic Bead Buffer.
  • the washing buffer and any non-adherent cells are removed and added to the mDC supernatant.
  • Samples from the mDC supernatant and wash buffer are removed for cell count, TB viability and immunopheno typing.
  • the adherent mDC are detached and harvested from the Cell Factories by adding ice-cold Magnetic Bead buffer and left resting for 30 minutes on frozen gel packs. After the 30 minutes, the mDC are harvested and the Cell Factories are washed once with ice-cold Magnet bead buffer. The Cell Factories are inspected microscopically to determine whether all the mDCs are removed. If it is observed that not all the mDCs are removed the wash process is repeated. The harvested (adherent) mDCs suspension is then centrifuged, washed and resuspended in Magnetic Bead Buffer. Samples from the adherent mDCs are removed for cell count, TB.
  • the cell concentration is adjusted to 1 x 10 7 cells/ml for peptide loading. In-process samples for immunophenotyping are removed. Once the volume and TNC of each fraction is determined the mDC supernatant fraction is discarded and the mDCs cell preparation of one embodiment is further processed.
  • the calculated peptivators (AdV5 Hexon, HCMV pp65, EBV select and BKV LT) are added to the cells and are incubated for 1 hour at 37 °C, 5 % CO2. Following the incubation the viral peptide loaded mDCs are washed and centrifuged with Magnetic Bead Buffer. Then the viral peptide loaded mDCs are resuspended with T cell Growth Medium and irradiated with 30-25 Gy via X-Ray source.
  • the viral peptide loaded mDCs are washed, centrifuged and resuspended in T Cell Growth Medium. Samples are removed for cell count, TB viability, immunophenotyping and sterility.
  • Fraction II CD4 CD56 CD45RA
  • the cells are retrieved from the incubator, centrifuged and resuspended in T Cell Growth Medium. Samples for cell count, TB viability, immunophenotyping and sterility are removed.
  • the cells from Fraction II (CD4 CD56 CD45RA ), are then washed and seeded in G-Rex ® 100 at a concentration of 1 x 10 6 cells/ml (100 ml/G-Rex ® 100 ) together with the viral peptide loaded mDCs at a ratio of 5:1 (Fraction II Cells: Dendritic Cells (DC)) in T Cell Growth Media along with IL-21 (100 IU/mL).
  • the co-culture cells are incubated for 3 days in 37 °C, 5 % CO2 for the starvation period.
  • a sample from the supernatant of the G-Rex ® 100 is carefully removed without disrupting the cell layer, to determine the pH and glucose level of the culture.
  • the pH should be at the physiologic range (pH 7.2-7.6) and the glucose at least 50 mg/dl.
  • Fresh T cell growth medium supplemented with IL-7 (30 IU/mL), IL-15 (125 IU/mL) and IL-21 (100 IU/mL) is added to each G-Rex ® 100 at 50 % of the culture volume. The cells are then incubated for an additional 48 hours at 37 °C with 5 % CO2. This process is repeated every 72 hours until the end of culture.
  • the cells are in culture for up to 12 days. Fresh media containing cytokines or only cytokines are added every 48 hours depending on the pH and glucose level. If the glucose level is between:
  • Glucose Level of 170 mg/dL to 130 mg/dL only cytokines IL-7, IL-15, IL-21 are added to the culture.
  • Glucose Level of 129 mg/dL to 100 mg/dL 100 ml (25 % of the G-Rex ® 100 volume) of fresh T cell growth medium + cytokines IL-7, IL-15, IL-21 are added to the culture.
  • Glucose Level of 99 mg/dL to 50 mg/dL 200 ml (50 % of the G-Rex ® 100 volume) of fresh T cell Growth medium + cytokines IL-7, IL-15, IL-21 are added to the culture.
  • the maximum volume level in the G-Rex ® 100 is 400 mL.
  • the volume of culture is replenished if the maximum volume in the G-Rex ® 100 is reached.
  • the Tcm cells On Day 15, the Tcm cells, inspected for visual contamination, harvested and washed with cell suspension media (Plamalyte-A/ 0.5% of 25% HSA). Samples are removed for non-release testing: cell count, TB viability, sterility and release-testing: endotoxin and mycoplasma prior to wash of the cells.
  • the cell preparation of one embodiment is washed and resuspended, release testing samples for cell count, viability, and immunophenotyping are removed. Following the cell count and TNC determination, the cell preparation of one embodiment is resuspended in approximately 50-100 mL of cell suspension media to a Tcm cell dose according to the dose-prescribed in the clinical protocol. Samples for release testing are obtained for gram stain and sterility as non-release testing. The final Tcm cell preparation meets release criteria prior to release of the cell preparation.
  • the final Tcm cells are transported from the CTL to the patient floor by the CTL staff in a plastic cooler to protect the cell preparation from temperature fluctuations and physical damage.
  • the CTL Staff deliver and issue the cell preparation to infusing personnel.
  • the mobilized hematopoietic progenitor cells (also termed HPC- A) is collected in three days.
  • a back-up fraction of unmodified PBMC containing 1 x 10 6 CD34 + cells per kg ideal body weight is set aside and cryopreserved.
  • the remaining first and second day‘s collection are pooled, CD34 + selected and cryopreserved.
  • the third day’s collection is depleted of CD3 + /CD19 + cells and cryopreserved.
  • the maximum T cell dose in the entire T cell depleted stem cell transplant collected from all fractions is 2 x 10 5 CD3 + cells per kg ideal body weight.
  • the CliniMACS ® PBS/EDTA Buffer bags 1000 ml containing 0.5 % Human Serum Albumin (HSA) are prepared prior to processing according to well-established laboratory SOP. The weight of the HPC-A preparation is determined. Samples from Day 1 and Day 2 of the HPC-A are removed for the following tests: cell count, viability, sterility, immunopheno typing. Each buffer and cell preparation bag is labeled with Patient’s name, MDACC number, and date of preparation. A back-up fraction of 2 x 10 6 CD34 + cells per kg ideal body weight is removed from the first day’s collection before the CD34 + selection procedure is performed on the remaining PBPC cell preparation. The remaining cells of the first day collection are kept overnight and pooled with the second day collection for the CD34 + enrichment process.
  • HSA Human Serum Albumin
  • the pooled HPC-A cell preparations of one embodiment are incubated with the CD34 reagent (CD34 antibody conjugated to super-paramagnetic particles).
  • the cells are washed to remove excess reagent. Cell count and immunophenotyping samples are removed.
  • the CD34 labeled cells are then processed on the CliniMACS ® using the CliniMACS ® tubing set and the CD34 + selection program. In this case, the magnetically labeled cells (CD34 + ) are retained by the column and CD34 negative cells are removed.
  • the CD34 + cells are then released from the column and collected. Samples from each fraction are removed for cell count, viability and immunopheno typing .
  • the CD34 + enriched cell preparation may be cryopreserved for infusion (as discussed below) or may be used as fresh cells (as discussed above).
  • the maximum TNC to process with one reagent kit and tubing set is 6 x 10 10 unless higher number is approved by the Laboratory Director or designee.
  • the maximum load volume for the CliniMACS ® instrument is 300 ml.
  • CliniMACS ® PBS/EDTA Buffer bags 1000 ml containing 0.5 % Human Serum Albumin (HSA) is prepared prior to processing according to well-established laboratory SOP. The weight of the HPC-A cell preparation is determined. Samples from the HPC-A are removed for the following tests: cell count, viability, sterility, and immunopheno typing. Each buffer and cell preparation bag is labeled with Patient’s name, Medical Record Number (MRN), and date of preparation.
  • MRN Medical Record Number
  • the HPC-A cell preparation of one embodiment is platelet depleted prior to the CD3 + /CD19 + depletion using the COBE 2991. Once the cells are removed from the COBE 2991, samples for cell count are removed. The cell preparation is then incubated with the CD3 reagent and CD 19 reagent.
  • the post-platelet depletion HPC-A cell preparation of one embodiment is incubated with IVIg for 10-15 minutes. After the initial incubation the CD3 + and CD19 + reagents (CD3 and CD19 antibodies conjugated to super-paramagnetic particles) are added to the cell preparation and incubated for 30 minutes on an orbital rotator. At the end of the incubation, the cell are washed using the COBE 2991 to remove excess reagent. Cell count and immunophenotyping samples are removed. The CD3 + /CD19 + labeled cells are then processed on the CliniMACS ® using the depletion tubing set and the depletion program.
  • CD3 + and CD19 + reagents CD3 and CD19 antibodies conjugated to super-paramagnetic particles
  • the magnetically labeled cells (CD3 + /CD19 + ) are retained by the column and CD3 and CD19 negative cells pass through the column and are collected as a CD3 + /CD19 + depleted fraction. Samples from each fraction are removed for cell count, viability and immunophenotyping.
  • the CD3 + /CD19 + depleted cell preparation of one embodiment is cryopreserved for infusion (as discussed below) or may be used as fresh cells (as discussed above).
  • the maximum TNC to process with one reagent kit and tubing set is 8 x 10 10 unless higher number is approved by the Laboratory Director or designee.
  • a second cycle of CD3 depletion may be performed on the CD3 + /CD19 + depleted to further reduce the CD3 population in the cell preparation. Samples for cell counts and immunophenotyping, viability and sterility are obtained from each fraction.
  • the cell preparations of one embodiment are concentrated and cryopreserved according to procedures validated at the CTL. A final cell count, viability, immunophenotyping, gram stain and sterility sample is obtained prior to cryopreservation of the cell preparation. Following the cell count and TNC determination, the cell preparation of one embodiment is cryopreserved in freeze media (50 % Plasma-Lyte A, 7.5 % DMSO, and 35 % HSA 25 %) according to SOP. Bags containing the CD34 + Enriched cells or CD3 + /CD19 + Depleted cells are frozen in a controlled rate freezer and stored in a liquid nitrogen freezer in vapor phase.
  • the final cell preparation is the preparation before cryopreservation as the thawing of the cells occurs at bedside without further manipulation.
  • the CD34 + Enriched cells and/or CD3 + /CD19 + Depleted cell preparations are delivered to the patient floor by the CTL staff and issued to the infusing personnel.
  • the final cell preparations meet lot release criteria prior to release of the cell preparations. These cells are then infused into the patient according to protocol specific dose.
  • protocol specific dose For note, when cryopreserved are used, the cells are thawed on the floor and released to infusing personnel per standard operating procedure. Also, when cryopreserved are used, the final cell preparations are transported from the CTL to the floor by CTL staff in an approved styrofoam LN2 transportation container with seamless metal inserts that are be filled with approximately 2 inches of liquid nitrogen.
  • Table 3 List of reagents, manufacturers, status (Licensed, Certificate of Analysis (CoA), or
  • Reagents are protocol specific and stored according to manufacturer’s recommendations. All reagents are qualified prior to the initiation of cell preparation manufacturing.
  • the inventory control of reagents includes documentation of receipt, reagent documentation review, tracking by the inventory manager; and review and release by QA as appropriate. Documentation for all FDA approved reagents is available through the MDACC Pharmacy. Certificates of Analysis and Cross- reference Letters for unapproved reagents must be obtained from the supplier. MSDS are managed as part of the Laboratory Safety Policy.
  • the reagents are removed from the final cell preparation. This is accomplished using various cell washing, concentrating, and re-suspension procedures.
  • BD BACTEC The MDACC Department of Laboratory Medicine tests In-Process and final cell preparation samples using this automated microbial detection system. All test are held for 14 days or until the cell preparation becomes positive. Test results are obtained before release of the cell preparation.
  • the number and timing of testing is a factor of the amount of manipulations performed on the cells, culture time, and test requirements.
  • the samples to be tested must be optimal for the type of test. Sample conditions are validated prior to the initiation of manufacturing to insure that no part of the culture media or other buffers interfere with the sterility assay. A minimum sample volume of 0.5 ml/test is required.
  • the cryopreserved Tcm cell preparation of one embodiment is tested for the presence of Mycoplasma.
  • the Tcm cell preparation (cryopreserved) is tested by a Gel Endpoint Polymerase (PCR) based assay.
  • the PCR assay is performed by an approved licensed reference laboratory.
  • the final Tcm cells are also tested using an approved biochemical mycoplasma rapid detection test (MycoAlert, Lonza Walkersville, Inc.).
  • MycoAlert biochemical mycoplasma rapid detection test
  • the sample is taken at time of harvest before cell washing and contains both cell and media.
  • the sample volume is a minimum of 0.5 ml.
  • the MycoAlert result is used as an interim test release criteria while the mycoplasma PCR based assay is performed.
  • the mycoplasma PCR result is not used as a release criteria but is analyzed along with clinical responses to help identify criteria associated with positive clinical outcomes.
  • Mycoplasma result must be Negative.
  • the investigation and proposed corrections for a positive sterility testing includes: identification of the microorganism(s) and anti-microbial agent sensitivity testing; evaluation of the current procedure for collecting and processing the cell preparation to determine the step at which contamination could be introduced,; development of changes in procedures that assist in preventing sterility lapses in the future; and implementation of appropriate testing to ensure that such changes to procedures produce a sterile cell preparation.
  • the Attending Physician is responsible for notification, monitoring and treatment of the patient.
  • the Principal Investigator is responsible for notifying the appropriate regulatory agencies (FDA, IRB) of deviations and adverse events. Additionally, the sterility failure and result of investigation of the cause and any corrective actions is reported (in an information amendment submitted to the IND in a timely manner, e.g. within 30 calendar days after initial receipt of the positive culture test result).
  • All CTL cell preparations are labeled and stored as to ensure accurate identification and prevent mix-ups. All cell preparations are assigned a unique identifier number. Critical steps during cell preparation labeling and retrieval require verification by supervising personnel.
  • CD34 + Enriched and CD3 + /CD19 + Depleted Cell Preparations Flow cytometry is also used to identify the different cell populations that are infused back into the patient. In addition to CD34 + and CD3 + /CD19 + determination, the following subset markers are evaluated: CD3, CD4, CD8, CD 16/ CD56, CD19 CD14, CD45RO and CD37.
  • Tcm Cell Preparations Flow cytometry is also used to identify the different cell populations that are infused back into the patient. In addition to CD45, CD3, CD8, CD62L, and CD45RO determination the following subset markers are evaluated: CD4, CD56, CD16, CD95 and CD45RA.
  • Flow cytometry is used at multiple points to determine the cellular phenotype.
  • the Tcm Cell Preparation Flow cytometry marker for CD3 + CD8 + CD62L + CD45RO + 7AAD- of CD45 + cells must be > 30%.
  • CD34 + Enriched Preparation and CD3 + CD19 + Depleted preparation must have a minimal acceptable cell dose of greater than 6 x10 6 CD34 + cells per kg ideal body weight. All other immunophenotyping data are not used as release criteria but are analyzed along with clinical responses to help identify appropriate potency assay that may be associated with positive clinical outcomes.
  • Immunophenotyping by flow cytometry is used to measure several potential potency markers including the markers mentioned in the Purity section above. None of the other immunophenotyping data is used as release criteria but is analyzed along with clinical responses to help identify criteria associated with positive clinical outcomes.
  • a potency assay is developed throughout this Phase I and be established prior to the initiation of the Phase III trial.
  • Endotoxin levels are determined in the CTL Quality Control laboratory using the Limulus Amebocyte Lysate (LAL) assay. This method is validated for all cell/media combinations to be tested prior to clinical use. The final Tcm cell preparations are tested and results are part of the release criteria. A minimum of 0.5 mL of sample is required. Endotoxin level must be less than 5 EU/Kg ideal body weight of the recipient.
  • LAL Limulus Amebocyte Lysate

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Transplantation (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Virology (AREA)
PCT/IL2020/050865 2019-08-06 2020-08-06 Anti-viral central memory cd8+ veto cells in haploidentical stem cell transplantation Ceased WO2021024264A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
CA3149379A CA3149379A1 (en) 2019-08-06 2020-08-06 Anti-viral central memory cd8+ veto cells in haploidentical stem cell transplantation
CN202080069269.9A CN114466925A (zh) 2019-08-06 2020-08-06 单倍体相合的干细胞移植中的抗病毒中央型记忆cd8+反抑细胞
BR112022001988A BR112022001988A2 (pt) 2019-08-06 2020-08-06 Métodos para gerar uma população isolada de células indutoras da não doença do enxerto versus hospedeiro, para tratar uma doença, para reduzir a rejeição do enxerto e/ou a doença do enxerto versus hospedeiro e/ou para induzir tolerância específica do doador e para tratar um indivíduo, população isolada de células indutoras da não doença do enxerto versus hospedeiro, composição farmacêutica, e, uso da população isolada de células indutoras da não doença do enxerto versus hospedeiro
MX2022001578A MX2022001578A (es) 2019-08-06 2020-08-06 Memoria central antiviral cd8+ celulas veto en celulas madre haploidenticas trasplante.
AU2020326568A AU2020326568A1 (en) 2019-08-06 2020-08-06 Anti-viral central memory CD8+ veto cells in haploidentical stem cell transplantation
JP2022506965A JP2022543139A (ja) 2019-08-06 2020-08-06 ハプロタイプ一致幹細胞移植における抗ウイルスセントラルメモリーｃｄ８＋ベト細胞
EP20850208.8A EP4009991A4 (en) 2019-08-06 2020-08-06 ANTI-VIRAL CENTRAL MEMORY CD8+ VETO CELLS IN HAPLOIDENTIC STEM CELL TRANSPLANTATION
US17/633,294 US20230398214A1 (en) 2019-08-06 2020-08-06 Anti-viral central memory cd8+ veto cells in haploidentical stem cell transplantation
IL290337A IL290337A (en) 2019-08-06 2022-02-03 Vito cells against viruses with a central memory phenotype, and their use for allogeneic stem cell transplantation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962883164P 2019-08-06 2019-08-06
US62/883,164 2019-08-06

Publications (2)

Publication Number Publication Date
WO2021024264A2 true WO2021024264A2 (en) 2021-02-11
WO2021024264A3 WO2021024264A3 (en) 2021-04-15

Family

ID=74504020

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2020/050865 Ceased WO2021024264A2 (en) 2019-08-06 2020-08-06 Anti-viral central memory cd8+ veto cells in haploidentical stem cell transplantation

Country Status (10)

Country Link
US (1) US20230398214A1 (https=)
EP (1) EP4009991A4 (https=)
JP (1) JP2022543139A (https=)
CN (1) CN114466925A (https=)
AU (1) AU2020326568A1 (https=)
BR (1) BR112022001988A2 (https=)
CA (1) CA3149379A1 (https=)
IL (1) IL290337A (https=)
MX (1) MX2022001578A (https=)
WO (1) WO2021024264A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4055146A4 (en) * 2019-11-05 2023-10-18 Yeda Research and Development Co. Ltd USE OF VETO CELLS TO TREAT T-CELL-MEDIATED AUTOIMMUNE DISEASES

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA05008486A (es) * 2003-02-10 2005-11-17 Northwest Biotherapeutics Inc Celulas presentadoras de antigeno cd14+ cultivadas.
WO2007137300A2 (en) * 2006-05-23 2007-11-29 Bellicum Pharmaceuticals, Inc. Modified dendritic cells having enhanced survival and immunogenicity and related compositions and methods
US9738872B2 (en) * 2008-10-30 2017-08-22 Yeda Research And Development Co. Ltd. Anti third party central memory T cells, methods of producing same and use of same in transplantation and disease treatment
WO2013035099A1 (en) * 2011-09-08 2013-03-14 Yeda Research And Development Co. Ltd. Anti third party central memory t cells, methods of producing same and use of same in transplantation and disease treatment
JP6715482B2 (ja) * 2014-07-18 2020-07-01 国立大学法人京都大学 多能性幹細胞から免疫細胞療法用ｔ細胞を誘導する方法
EP3322424B1 (en) * 2015-07-16 2023-10-11 Yeda Research and Development Co., Ltd. Use of anti third party central memory t cells
IL263924B2 (en) * 2016-06-27 2025-03-01 Yeda Res & Dev Voto cells produced from memory T cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4055146A4 (en) * 2019-11-05 2023-10-18 Yeda Research and Development Co. Ltd USE OF VETO CELLS TO TREAT T-CELL-MEDIATED AUTOIMMUNE DISEASES

Also Published As

Publication number Publication date
US20230398214A1 (en) 2023-12-14
AU2020326568A1 (en) 2022-03-24
CA3149379A1 (en) 2021-02-11
WO2021024264A3 (en) 2021-04-15
MX2022001578A (es) 2022-04-26
IL290337A (en) 2022-04-01
CN114466925A (zh) 2022-05-10
EP4009991A4 (en) 2023-09-13
JP2022543139A (ja) 2022-10-07
EP4009991A2 (en) 2022-06-15
BR112022001988A2 (pt) 2022-05-10

Similar Documents

Publication Publication Date Title
US12391916B2 (en) Veto cells generated from memory T cells
EP2846816B1 (en) Methods and compositions for infusion of transiently engrafting, selected populations of allogeneic lymphocytes to treat cancer
US20220265725A1 (en) Use of veto cells in treatment of t cell mediated autoimmune diseases
US20220265726A1 (en) Use of veto cells for the treatment of sickle cell disease
US20230398214A1 (en) Anti-viral central memory cd8+ veto cells in haploidentical stem cell transplantation
RU2834894C2 (ru) Противовирусные клетки вето центральной памяти cd8+ при гаплоидентичной трансплантации стволовых клеток
WO2024095263A1 (en) Conditioning protocols for use with anti-viral central memory cd8 + veto cells in haploidentical stem cell transplantation
RU2779844C2 (ru) Вето-клетки, полученные из т-клеток памяти
CA3029001C (en) Veto cells generated from memory t cells
HK40007502A (en) Veto cells generated from memory t cells
HK40007502B (en) Veto cells generated from memory t cells

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20850208

Country of ref document: EP

Kind code of ref document: A2

ENP Entry into the national phase

Ref document number: 3149379

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2022506965

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112022001988

Country of ref document: BR

ENP Entry into the national phase

Ref document number: 2020850208

Country of ref document: EP

Effective date: 20220307

ENP Entry into the national phase

Ref document number: 2020326568

Country of ref document: AU

Date of ref document: 20200806

Kind code of ref document: A

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20850208

Country of ref document: EP

Kind code of ref document: A2

ENP Entry into the national phase

Ref document number: 112022001988

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20220202

WWG Wipo information: grant in national office

Ref document number: 2022105687

Country of ref document: RU