WO2021002987A9 - High-brightness nanodot fluorophores by covalent functionalization - Google Patents

High-brightness nanodot fluorophores by covalent functionalization Download PDF

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Publication number
WO2021002987A9
WO2021002987A9 PCT/US2020/035568 US2020035568W WO2021002987A9 WO 2021002987 A9 WO2021002987 A9 WO 2021002987A9 US 2020035568 W US2020035568 W US 2020035568W WO 2021002987 A9 WO2021002987 A9 WO 2021002987A9
Authority
WO
WIPO (PCT)
Prior art keywords
nanodot
fluorophores
brightness
covalent functionalization
carrier
Prior art date
Application number
PCT/US2020/035568
Other languages
French (fr)
Other versions
WO2021002987A1 (en
Inventor
Yoke Khin Yap
Chee HUEI
Original Assignee
Michigan Technological University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Michigan Technological University filed Critical Michigan Technological University
Priority to CN202080040136.9A priority Critical patent/CN113892026A/en
Priority to CA3139733A priority patent/CA3139733A1/en
Priority to KR1020217043403A priority patent/KR20220016920A/en
Priority to EP20746772.1A priority patent/EP3977125A1/en
Priority to US17/615,425 priority patent/US20220226509A1/en
Priority to JP2021570766A priority patent/JP2022534285A/en
Priority to PCT/US2020/047378 priority patent/WO2021035135A1/en
Priority to CA3148704A priority patent/CA3148704A1/en
Priority to EP20764562.3A priority patent/EP4018192A1/en
Priority to KR1020227009150A priority patent/KR20220057550A/en
Priority to JP2022511205A priority patent/JP2022544703A/en
Priority to CN202080074598.2A priority patent/CN114631016A/en
Priority to US17/637,018 priority patent/US20220291207A1/en
Priority to EP20792869.8A priority patent/EP4038385A1/en
Priority to PCT/US2020/053500 priority patent/WO2021067411A1/en
Priority to CA3152735A priority patent/CA3152735A1/en
Priority to JP2022519670A priority patent/JP2022549369A/en
Priority to CN202080068375.5A priority patent/CN114467026A/en
Priority to KR1020227014362A priority patent/KR20220070512A/en
Publication of WO2021002987A1 publication Critical patent/WO2021002987A1/en
Publication of WO2021002987A9 publication Critical patent/WO2021002987A9/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/02—Use of particular materials as binders, particle coatings or suspension media therefor
    • C09K11/025—Use of particular materials as binders, particle coatings or suspension media therefor non-luminescent particle coatings or suspension media
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1018—Heterocyclic compounds
    • C09K2211/1025—Heterocyclic compounds characterised by ligands
    • C09K2211/1088—Heterocyclic compounds characterised by ligands containing oxygen as the only heteroatom

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Materials Engineering (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Preparation (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A example compound according to the present disclosure includes, among other possible things, a nanodot carrier, a moiety, and a linker having first and second functional groups, wherein the first functional group is covalently linked to the nanodot carrier, and the second functional group is covalently linked to the moiety. An example method of making a nanodot carrier is also disclosed.
PCT/US2020/035568 2017-04-13 2020-06-01 High-brightness nanodot fluorophores by covalent functionalization WO2021002987A1 (en)

Priority Applications (19)

Application Number Priority Date Filing Date Title
CN202080040136.9A CN113892026A (en) 2019-05-31 2020-06-01 High brightness nanodot fluorophores through covalent functionalization
CA3139733A CA3139733A1 (en) 2019-05-31 2020-06-01 High-brightness nanodot fluorophores by covalent functionalization
KR1020217043403A KR20220016920A (en) 2019-05-31 2020-06-01 High Brightness Nanodot Fluorophores by Covalent Functionalization
EP20746772.1A EP3977125A1 (en) 2019-05-31 2020-06-01 High-brightness nanodot fluorophores by covalent functionalization
US17/615,425 US20220226509A1 (en) 2017-04-13 2020-06-01 High-brightness nanodot fluorophores by covalent functionalization
JP2021570766A JP2022534285A (en) 2019-05-31 2020-06-01 High-Brightness Nanodot Fluorescent Dyes with Covalent Functionalization
PCT/US2020/047378 WO2021035135A1 (en) 2019-08-21 2020-08-21 High-brightness fluorophores for quantification and phenotyping of extracellular vesicles
CA3148704A CA3148704A1 (en) 2019-08-21 2020-08-21 High-brightness fluorophores for quantification and phenotyping of extracellular vesicles
EP20764562.3A EP4018192A1 (en) 2019-08-21 2020-08-21 High-brightness fluorophores for quantification and phenotyping of extracellular vesicles
KR1020227009150A KR20220057550A (en) 2019-08-21 2020-08-21 High-brightness fluorophores for quantification and phenotyping of extracellular vesicles
JP2022511205A JP2022544703A (en) 2019-08-21 2020-08-21 Bright fluorescent dyes for extracellular vesicle quantification and phenotyping
CN202080074598.2A CN114631016A (en) 2019-08-21 2020-08-21 High brightness fluorophores for quantification and phenotypic analysis of extracellular vesicles
US17/637,018 US20220291207A1 (en) 2019-08-21 2020-08-21 High-brightness fluorophores for quantification and phenotyping of extracellular vesicles
EP20792869.8A EP4038385A1 (en) 2019-09-30 2020-09-30 Composite high-brightness fluorophores with controllable spectra shapes and method of using composite high-brightness fluorophores
PCT/US2020/053500 WO2021067411A1 (en) 2019-09-30 2020-09-30 Composite high-brightness fluorophores with controllable spectra shapes and method of using composite high-brightness fluorophores
CA3152735A CA3152735A1 (en) 2019-09-30 2020-09-30 Composite high-brightness fluorophores with controllable spectra shapes and method of using composite high-brightness fluorophores
JP2022519670A JP2022549369A (en) 2019-09-30 2020-09-30 Composite bright fluorochromes with controllable spectral shapes and methods of using composite bright fluorochromes
CN202080068375.5A CN114467026A (en) 2019-09-30 2020-09-30 Composite high brightness fluorophores with controllable spectral shape and methods of using the same
KR1020227014362A KR20220070512A (en) 2019-09-30 2020-09-30 Complex high-brightness fluorophores with controllable spectral morphology and methods of using complex high-brightness fluorophores

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962855121P 2019-05-31 2019-05-31
US62/855,121 2019-05-31

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2020/035574 Continuation-In-Part WO2020243710A1 (en) 2017-04-13 2020-06-01 High-brightness nanotube fluorophores by covalent functionalization

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US17/637,018 Continuation-In-Part US20220291207A1 (en) 2019-08-21 2020-08-21 High-brightness fluorophores for quantification and phenotyping of extracellular vesicles

Publications (2)

Publication Number Publication Date
WO2021002987A1 WO2021002987A1 (en) 2021-01-07
WO2021002987A9 true WO2021002987A9 (en) 2021-01-28

Family

ID=74100332

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2020/035568 WO2021002987A1 (en) 2017-04-13 2020-06-01 High-brightness nanodot fluorophores by covalent functionalization

Country Status (6)

Country Link
EP (1) EP3977125A1 (en)
JP (1) JP2022534285A (en)
KR (1) KR20220016920A (en)
CN (1) CN113892026A (en)
CA (1) CA3139733A1 (en)
WO (1) WO2021002987A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116239101A (en) * 2021-12-08 2023-06-09 深圳先进技术研究院 Nano enzyme with near infrared fluorescence and superoxide dismutase active carbon point, preparation method and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0404708D0 (en) * 2004-03-02 2004-04-07 Cellic As T Boron containing nanoparticles
US9840735B2 (en) * 2012-07-02 2017-12-12 Koninklijke Philips N.V. Barrier coated nano structures
CA3061892A1 (en) * 2017-04-13 2018-10-18 Michigan Technological University High-brightness fluorophores

Also Published As

Publication number Publication date
EP3977125A1 (en) 2022-04-06
CA3139733A1 (en) 2021-01-07
KR20220016920A (en) 2022-02-10
WO2021002987A1 (en) 2021-01-07
JP2022534285A (en) 2022-07-28
CN113892026A (en) 2022-01-04

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