WO2020230045A1

WO2020230045A1 - Liposuction aspirate and fat purification device

Info

Publication number: WO2020230045A1
Application number: PCT/IB2020/054521
Authority: WO
Inventors: Mario GOISIS
Original assignee: Goisis Mario
Priority date: 2019-05-13
Filing date: 2020-05-13
Publication date: 2020-11-19

Abstract

The invention relates to a method and a device (100) for purifying aspirate extracted from a patient, which comprises: - a first connector (101) able to be coupled with a containment member (102) for suction fluid, so as to receive its contents; - a discharge member (103), connected or connectable to a discharge tank (104), which is adapted to receive a waste fluid; - a duct (105) which connects the first connector (101) to the discharge member (103); - a washing tank (106), adapted to contain a washing liquid and connected or connectable to the duct (105) by means of a second connector (107), so as to introduce the washing liquid into the latter. Moreover, the following are provided, in sequence, along the duct (105) between the first connector (101) and the discharge member (103): - a first filtering member (108) configured to prevent the passage of mature cell elements and, instead, able to be crossed by adipocyte precursors and stem cells and by a stromal vascular fraction; - a third connector (109) able to be coupled with a receiving member (110) configured to receive fluid from said duct (105); - a second filtering member (111) configured to prevent the passage of precursors of vital cell elements and vital stem cells and, instead, able to be crossed by blood and/or washing liquid.

Description

LIPOSUCTION ASPIRATE AND FAT PURIFICATION DEVICE

Technical sector

The present invention relates generally to medicine and aesthetic surgery and in particular to a device for purifying cells and fat extracted by liposuction and aspiration.

Background art

Liposuction was introduced during the 1970s. Initially it was a very invasive procedure carried out under general anesthesia in major surgery rooms. The procedure lasted several hours and the excess fat was removed by means of aspiration using large cannulas with a diameter of 1-2 cm connected to a high-power aspirator. The fat was eliminated together with large amounts of blood and anesthetic. In many cases, blood transfusions were required during the days following the operation and many complications and deaths are reported in medical literature.

Only since the 1990s have less invasive liposuction techniques gradually become established. The size of the cannulas has gradually been reduced down to average diameters of 2-5 mm.

The introduction of the tumescent anesthesia technique has also allowed many operations to be carried out under local anesthesia. With the tumescent technique a large volume of liquid containing a local anesthetic together with vasoconstrictor (adrenaline) is injected into the area to be treated. This injection reduces the bleeding and the pain during extraction and makes liposuction simpler and less dangerous.

During the last 20 years so-called "lipofilling" treatments have been developed, these making use of the aspirated fat as a filler for reshaping and increasing the volume of the breast, buttocks and the calf. In the region of the face, wrinkles may be filled and corrected, and the lips, cheekbones and chin may be reshaped.

It has therefore become necessary to preserve as far as possible the integrity of the fat removed. Unlike the initial liposuction procedures, in fact, the fat is no longer eliminated, but becomes a resource for aesthetic treatment procedures. The effectiveness of this resource is strictly related to the survival of the graft, namely transfer of the fat from a donor site to a target site. The fat is formed by adipocytes, stem cells and adipocyte precursor cells. Each of these elements contributes towards ensuring the success of the treatment.

These cells are removed from the site where they are in excess (thighs, abdomen, hips) and are grafted into the site to be reshaped (breast, lip, etc.). The procedure is extremely delicate and always involves the death of a variable percentage of the grafted cells. The survival rate varies between 70% in the best case scenario to less than 10%, which obviously results in failure of the treatment.

The survival of the fat is associated with numerous factors: the individual predisposition, the degree of oxidation of the tissues, the infiltration technique, the fact that the patient is a smoker, the trauma caused by the cannula during the extraction process, etc. The local anesthetic used to numb the infiltration zone represents undoubtedly one of the most important damaging factors for the grafted cells since it has a toxic effect on the cells during the 12-24 hours following their implantation in the target site. This damage is increased by the presence of erythrocytes which, if left together with the fat, have a significant oxidative effect. In order to reduce tissue damage recently smaller size cannulas have been introduced, these being able to be easily inserted into the adipose tissues and reducing the force which must be applied to them. Cannulas with special arrangements and shapes of the holes through which the fat is sucked have also been developed. An example of such a cannula is described in Italian patent application 102015902323792 in the name of the present Applicant.

During a liposuction procedure it is required to inject an anesthetic solution into the site from where the fat is to be extracted. The anesthetic is generally injected by means of syringes.

Once the anesthetic has been injected, the same syringes are used to draw off the graft following the mounting of suitable liposuction cannulas. The extracted graft is obviously mixed with local anesthetic and blood (referred to as "liquid component"). Generally the difference in specific weight is used to separate the fat from the liquid component, with the syringes being vertically positioned manually or by means of suitable rack-like supports. The graft, which has a lower specific weight, is collected in top part of the syringe, i.e. towards the plunger, while the liquid component is deposited in the bottom part of the syringe, where the cannula is attached, and may thus be eliminated. In order to ensure the survival of the cells grafted with a view to a subsequent lipofilling treatment, the graft remaining inside the syringe must be treated during one or more cycles with a washing liquid, typically a physiological solution, so as to purify it of the residual blood and anesthetic.

Blood and anesthetic are in fact toxic substances for the graft. In particular, during each treatment cycle, a certain amount of washing liquid is sucked into the syringe, which is then positioned vertically in order to allow the separation of the fat and the liquid component. The liquid component, which is deposited on the bottom owing to the greater specific weight, is then eliminated and a new washing solution is sucked into the syringe again and the separation and washing procedure repeated.

A number of different containers, which must be suitably prepared and organized before an intervention, are generally used for the aforementioned operations, the successful outcome of which depends greatly on the manual dexterity of the surgeon. For example, a first container is filled with physiological solution, a second container is filled with anesthetic and a third container is used to collect the liquid component to be eliminated during each treatment cycle. These containers are generally open to the external atmosphere and are thus exposed to the risk of contamination by bacteria present in the operating theatre.

Italian patent No. 102015000061679, in the name of the present Applicant, describes an invention aimed at improving the procedures for purifying the fat extracted by means of liposuction with particular reference to the devices used therein.

Said invention consists of a filtering device comprising a filter element on one side of which a connection element is arranged, it being possible to connect to said element a syringe containing fat extracted from a patient by means of liposuction treatment. The syringe also contains the liquid component consisting of blood and anesthetic mixed together with the fat. On the opposite side of the filter element a first reservoir containing a physiological solution and a second, empty, reservoir are connected in parallel by means of a three-way connector. The filter element is configured to retain the fat cells and to allow the liquid component to pass through.

This device is configured so that, by injecting the contents of the syringe towards the filter element, the liquid component which crosses it is directed towards and collected inside the second, empty, reservoir, whereas, by operating the plunger of the syringe in the opposite direction, the syringe draws in the washing liquid from the first reservoir through the filtering element. The washing liquid is therefore mixed together with the fat and the residual matter contained inside the syringe. A specific system of one-way valves, taps and/or filters ensures the tightness of the system, resulting in a reduction in the risks of contamination and consequent infection.

By repeating cyclically the injection and suction steps, the fat contained inside the syringe is therefore gradually purified of the residual blood and anesthetic.

Although this device and method are widely accepted, there exists a particularly urgent need to improve the purification technique and efficiency.

Further purification devices and methods according to the preamble of the attached independent claims are substantially described in the documents US2016/361476A1, US2005/048033A1, US2010/285521A1 and JP2010/088386A.

The problem underlying the present invention is therefore that of improving and selecting the purification of the adipocytes and the dermic and subcutaneous tissue including the stromal vascular fraction and the stem cells extracted by means of aspiration (which will be referred to below as "aspirate" or "aspirated material").

The task of the present invention is therefore to solve this problem by providing a device and method for purifying fat which allows different cellular components present in the aspirate, such as the mature adipocytes and adipocyte precursors and stem cells, to be selected and concentrated.

In connection with this task, an object of the present invention is to provide a fat purification device and method which is able to simplify the procedures for enriching the aspirate with stromal vascular component, stem cells and platelet rich plasma (PRP).

A further object of the present invention is to provide a device and method for purifying liposuction fat, which are able to form a liposuction fat emulsion, known in scientific literature as "nanofat", which is richer in stem cells than the emulsions which can be obtained by means of conventional devices and methods.

Yet another object of the present invention is to propose a device and method for purifying liposuction fat, which are able to form nanofat, which is richer in live cells than the nanofat which can be obtained by means of conventional devices and methods. This task, as well as these and other objects which will become clearer below are achieved by a device and method for purifying liposuction fat according to the attached independent claims.

Detailed description

Detailed characteristic features of a device and method for purifying liposuction fat according to the invention are contained in the dependent claims.

Further characteristic features and advantages will emerge more clearly from the description of a preferred, but non-exclusive embodiment of a device and method for purifying liposuction fat according to the invention, shown by way of a non-limiting example in the attached sets of drawings in which:

- Figure 1 shows a simplified structural diagram of a liposuction fat purification device according to the present invention;

- Figure 2 shows a simplified structural diagram of a variation of embodiment of a liposuction fat purification device according to the present invention.

With particular reference to the said figures, 100 denotes overall a liposuction fat purification device which comprises:

- a first connector 101 able to be coupled to a containment member 102 for receiving its contents, the containment member 102 being configured to contain a liposuction fluid;

- a discharge member 103, connected or connectable to a discharge tank 104 and adapted to receive a waste fluid, for example consisting of or comprising a syringe or a bag, whereby a member may be provided for preventing the reflux of waste fluid into the duct 105 through the discharge member 103;

- a duct 105 which connects the first connector 101 to the discharge member 103;

- a washing tank 106, adapted to contain washing liquid and connected or connectable to the duct 105 by means of a second connector 107, so as to introduce washing liquid into the latter, whereby a member may be provided for preventing the reflux of washing liquid into the washing tank through the connector 107.

According to a particular aspect of the present invention, the device 100 comprises, in sequence along the duct 105, between the first connector 101 and the discharge member 103: - a first filtering member 108 configured to prevent the passage of mature cells and especially mature adipocytes and, instead, able to be crossed by adipocyte precursors and stem cells;

- a third connector 109 able to be coupled with a receiving member 110, which for example may be or comprise a syringe, configured to receive fluid from the duct 105;

- a second filtering member 111 configured to prevent the passage of vital adipocyte precursors and vital stem cells and, instead able to be crossed by blood and/or washing liquid.

In this way a device 100 according to the present invention offers a surgeon a proper aspirate selection and purification device which, owing to the provision of more than one filtering member, allows all the operations typically performed by means of more complex conventional devices to be performed in a reliable and repeatable manner.

In particular, by means of the device 100 according to the present invention, it is possible to select various cellular components present in the aspirated material, such as the mature adipocytes, which are characterized by a very large diameter, and the adipocyte precursors and stem cells, which are characterized by an intermediate diameter, and the erythrocytes and platelets, which are characterized by a small diameter.

As will emerge more clearly from the description below, moreover, the device allows the production of an emulsion which is rich in stem cells, referred to in the literature as "nanofat", particularly rich in stromal vascular component, and the injection of the anesthetic solution, the extraction of the aspirated material, the washing/filtering and enrichment thereof, the production of nanofat and grafting thereof, in a completely closed cycle.

The present invention also relates to a method for purifying liposuction fat, in order to produce fat purified by means of a device 100 according to the present invention. Said method comprises the following steps, in succession:

A. forcing a flow of aspirated material, for example obtained by means of liposuction, to pass, in succession through the first filtering member 108, so as to filter a first fraction of aspirated material, and the second filtering member 111, so as to filter a second fraction of aspirated material and discharge subsequently a waste fraction of aspirated material, without said first and second fractions, through the discharge member 103, for example into a discharge member 103;

B. aspirating, into the duct 105, from the washing tank 106 a washing liquid and mixing it with the first fraction and/or with the second fraction, so as to dilute the anesthetic and the blood which are generally contained inside it;

C. forcing a mixture of the washing liquid with the first fraction and/or with the second fraction through the filtering members 108 and 111, so as to obtain a first purified fraction rich in cellular elements, and especially vital mature adipocytes, a second purified fraction rich in vital adipocyte precursors and vital stem cells, and a waste fraction; wherein the first purified fraction and the second purified fraction are respectively filtered by the first filtering member 108 and by the second filtering member 11, the waste fraction being forced through the discharge member 103, for example into the discharge member 103;

D. repeating cyclically steps B and C, for example until the desired degree of purification of the first fraction and/or the second fraction is obtained.

According to one aspect of the invention, according to step B, the washing liquid may be sucked into the containment member 102, so as to be mixed with the aspirated material contained therein, or into the receiving member 110, so as to be mixed with the aspirated material collected between filtering members 108 and 111.

According to an aspect of the invention, the aforementioned method may also comprise, following said step D, a step of:

E. emulsifying the first purified fraction to obtain nanofat.

According to another aspect of the invention, the aforementioned method may also comprise, following said step E, a step of:

F. adding said first purified fraction, or a derivative thereof which may be emulsified, to said second purified fraction, so as to form a nanofat enriched with vital adipocyte precursors and with vital stem cells.

According to one aspect of the invention, the first filtering member 108 may be configured to prevent the passage of particles having dimensions not smaller than a first dimension chosen in a range of between 50 microns and 400 microns.

The first filtering member 108 may be configured to prevent the passage of particles having dimensions not smaller than a first dimension chosen in a range of between 20 microns and 400 microns. Moreover, the second filtering member 11 1 may be configured to prevent the passage of particles having dimensions not smaller than a second size chosen in a range of between 5 microns and 50 microns.

The second filtering member 111 may be configured to prevent the passage of particles having dimensions not smaller than a second dimension chosen in a range of between 5 microns and 80 microns.

According to an aspect of the invention, the washing tank 106 may be connected to the duct 105 by means of a valve member 112 configured to allow the outflow from the washing tank 106 and prevent reflux into the said washing tank 106. The valve member 112 may be a non-return valve, for example of the conventional type not further described.

According to one aspect of the invention, the filtering members 108 and 11 1 are arranged, along the duct 105, between the first connector 101 and the second connector 107, as shown for example in the embodiment of the invention according to Figure 1.

In this way, during use, a flow of washing liquid from the washing tank 106 towards the containment member 102 will pass through the filtering members 108 and 111

Thus, during the step B, the washing liquid, sucked from the washing tank, will cross the filtering member 108 and 111 in the opposite direction to the direction of flow of the aspirated material which is forced to cross them during the steps A or C. This crossing movement in an opposite direction of flow allows cleaning of the filtering members so as to protect the functionality thereof and improve the purification efficiency.

According to another aspect of the present disclosure, the second connector 107 may instead be arranged, along the duct 105, between the first connector 101 and the filtering members 108 and 111, or so that the first connector 101 is positioned between the second connector 107 and the filtering members 108 and 111, as for example in the embodiment of the invention shown in Figure 2.

The second connector 107, in this case, will be positioned so that a flow of washing liquid directed from the washing tank 106 towards the containment member 102 does not cross the filtering members 108 and 111.

According to an aspect of the present disclosure, the device 100 may comprise an auxiliary container 113 arranged in fluid communication with the duct 105 by means of a fourth connector 114.

The second connector 107 may in this case be positioned, along the duct 105, between the fourth connector 114 and the first connector 101;

The device 100 may also comprise pressure limiting means, configured to prevent an outflow, from the auxiliary container 113, of a fluid having a pressure lower than a first threshold; there also being provided pressure limiting means configured to prevent reflux, towards the auxiliary container 113, of a fluid having a pressure less than a second threshold, where said first threshold is less than said second threshold.

The pressure limiting means may consist of, or comprise, a two-way non-return valve 115 positioned on the duct 105 between the first connector 101 and the fourth connector 114.

According to an aspect of the present disclosure, the step B may involve simultaneously sucking washing liquid from the washing tank 106 and aspirated material from the containment member 102, collecting them inside the auxiliary container 113 so as to perform mixing thereof.

In this respect, the auxiliary container 113 may be a syringe for facilitating such a simultaneous sucking action.

Accordingly, the step C may involve emptying of the auxiliary container 113 in order to force its contents through the filtering members 108 and 111.

According to an aspect of the invention, the device 100 may comprise:

- a liposuction syringe 116, which can be connected to the duct 105 in order to transfer aspirated material during a liposuction treatment, from the liposuction syringe 116 to the containment member 102,

and/or

- a lipofilling syringe 117, connected to the duct 105 for receiving from the latter purified fat and especially nanofat which may be enriched, as resulting from step E and/or F of the aforementioned purification method.

In this case, it is possible to implement the aforementioned purification method, from step A to step F, without exposing the aspirate to the environment, thus helping prevent contamination of the aspirate.

According to an aspect of the present disclosure, the device 100 may also comprise an oscillating and/or vibrating base plate - conventional per se and not shown in the attached figures - on which the duct 105 may be fixed so as to subjected to oscillations and/or vibrations; said oscillating and/or vibrating base plate being configured to generate oscillations and/or vibrations suitable for carrying out the separation of stem cells from stromal vascular material in an aspirated material present, during use, in the device 100.

In the figures, the zones for joining together the various components of the device 100 are schematically indicated by means of areas highlighted with parallel lines and may comprise valves for intercepting and/or regulating the flow, for example taps.

In order to perform the purification treatment of the aspirated material collected inside the containment member 101 which, as shown for example in the embodiment of Figures 1 and 2, may be a liposuction syringe, the plunger of the syringe may be lowered so as to force the aspirated material through the filtering members 108 and 111.

A first fraction of the aspirated material, which is rich in mature adipocytes, will be retained by the first filtering member 108, a second fraction, which is rich in vital adipocyte precursors and vital stem cells, will be retained by the second filtering member 111, while the resultant waste fraction, which is rich in blood and anesthetic, will be discharged through the discharge member 103.

It can be understood how the valve member 112 prevents the entry of the waste fraction into the washing tank 106, while the said waste fraction is pushed towards the discharge member 103.

The latter may in turn be provided with a non-return valve so as to prevent reflux of the waste fraction into the duct 105.

When the plunger is retracted, washing fluid will be recalled from the washing tank 106 and will be mixed with the first fraction and/or the second fraction, diluting the blood and anesthetic contained in them.

Clearly, instead of operating the plunger of the containment member 102, if the auxiliary container 113 is provided, it will be possible to recall into it the aforementioned fractions together with the washing liquid.

In this connection, for the sake of simplicity, the auxiliary container 113 may be, or comprise, a syringe.

It will be understood how the non-return valve of the discharge member 103 will prevent the reflux of the waste fraction into the duct 105.

If the syringe or - in general - the containment member 102 or the auxiliary container 113 are discharged, in the cases described above, a waste fraction will again be discharged, while collecting a first and a second fraction purified, respectively, at the first filtering member 108 and at the second filtering member 111. During these steps the purification may be increased.

In order to ensure a better outcome of the purification treatment, the device 100 according to the invention may comprise a microfilter 118 arranged between the washing tank 106 and the valve member 112.

The microfilter 118, which is for example provided with meshes having apertures in the region of fractions of microns (for example 0.2 microns), is able to retain any impurities and bacteria which are present in the washing liquid and which otherwise would be mixed with the fractions to be purified.

Still with regard to ensuring the best outcome of the purification treatment, the device 100 may also comprise a safety valve 119 set to open beyond a predefined pressure threshold.

This safety valve, which is schematically indicated by the reference number 119, is branched off from the first connector 101.

The use of a safety valve is able to limit the maximum pressure for injection of the fat towards the filtering members 108 and 111 and, therefore, the mechanical compression of the fat cells against its meshes, ensuring a greater probability of survival during a purification treatment.

The safety valve 119 may be advantageously associated with a pressure sensor (not shown) which is provided with a visual and/or acoustic indicator and which allows a user to reduce the pressure exerted in order to force the aspirate through the filtering members 108 and 111, should the predefined pressure threshold be exceeded.

The first fraction present in the first filtering member 108 may, at the end of purification, be collected inside a container (not shown) or, more advantageously, in a further syringe, for example in the lipofilling syringe 117.

In this way the fat present in the first filtering member 108 will not be lost.

Again with a view to ensuring the best outcome of the purification treatment and reducing as far as possible the fat loss in the system, the device 100 may also comprise two taps positioned immediately upstream and downstream of each filtering member 108 and 111, and a third tap provided with a luer lock connector or the like.

According to a further aspect of the invention, the discharge tank 104 may contain an element 121 made of or comprising an absorbent material. In this way the washing liquids and blood may be disposed of in a simple and rapid manner.

According to an embodiment of the invention, this element made of absorbent material is contained in a film of hydrosoluble material in turn arranged in a disposable bag, as described in the patent publication EP 2452660 A1.

A sensor 120 able to measure relative percentages of fat and washing liquid may be advantageously connected to the first connector 101. The sensor may comprise for example an electric impedance, an infrared measuring device of the NIR (Near-Infrared Interactance) type, an electromagnetic field generator of the TOBEC (Total Body Electrical Conductivity) type, or an X-ray system of the (DEXA Dual Energy X-ray Absorptiometry) type.

The device 100 according to the invention may be advantageously positioned on a scales for measuring the weight of the quantity of aspirated material mixed together with liquid component removed from the patient and to be purified.

According to a further aspect of the invention, the discharge tank 104 may comprise an outlet opening 121 for discharging the liquid component.

The outlet opening 121 is preferably arranged close to the bottom of the discharge tank 104 so as to facilitate emptying thereof. A filtering element configured for filtration of specific cellular elements which cross the said filtering element together with the waste fraction may be advantageously associated with the outlet opening 121. The filtering element may, for example, have through-openings in the range of 12-180 microns.

With reference now to Figure 2, an alternative embodiment of the device 100 according to the present invention, configured for the assembly, in parallel, of a plurality of syringes, will be described.

In a similar manner to the first embodiment of the invention, in this case also, the device 100 comprises a washing tank 106 filled with a washing liquid and a second, empty, discharge tank 104 intended to receive cyclically a waste fraction consisting of the washing liquid, blood and anesthetic, separated from the fat.

A collection container 122 may be connected to the duct 105 for transferring cyclically between the latter and the containment member 102, or the auxiliary container 113 if present, the first fraction purified so as to emulsify and obtain nanofat.

At the end of emulsification, the emulsion obtained may be pushed through the first filtering member 108 into a third syringe or receptacle or container, connected to the third connector 109.

The third connector 109 may have, connected thereto, the receiving member 110 for collecting the second purified fraction which is rich in vital adipocyte precursors and vital stem cells which are to be mixed with the first emulsified fraction to obtain enriched nanofat.

Since the quantity of vital elements improves the clinical results of the nanofat grafting treatment, the graft carried out using aspirate obtained with the technique described here is able to ensure greater success compared to conventional treatments which use aspirate obtained according to known methods.

Even better results are obtained by the treatments with nanofat enriched with cellular elements, as described above.

In particular, as a result of the present technical invention, the stem cells and the stromal vascular component present between the two nanofat filtering members may be mixed together in a very simple manner.

The invention has been described hitherto with reference to examples of embodiment shown in the attached figures. It is to be understood, however, that other embodiments relating to the same inventive idea may exist, as defined by the scope of protection of the claims which are illustrated hereinbelow.

The invention thus devised may be subject to numerous modifications and variations, all of which fall within the scope of protection of the accompanying claims.

All the details may be replaced by other technically equivalent elements.

In practice the materials used as well as the associated forms and dimensions may be varied depending on the particular requirements and the state of the art.

Where the constructional characteristics and the techniques mentioned in the following claims are followed by symbols or reference numbers, these reference numbers or symbols have been assigned with the sole purpose of facilitating understanding of the said claims and consequently they do not limit in any way the interpretation of each element which is identified, purely by way of example, by said reference numbers or symbols.

Claims

1. Device (100) for purifying fat and/or stromal vascular component and/or stem cells obtained by aspiration, said device (100) comprising:

- a first connector (101) able to be coupled to a containment member (102) for suction fluid, so as to receive its contents;

- a discharge member (103), connected or connectable to a discharge tank (104), which is adapted to receive a waste fluid;

- a duct (105) which connects said first connector (101) to said discharge member

(103);

- a washing tank (106), adapted to contain a washing liquid and connected or connectable to said duct (105) by means of a second connector (107), so as to introduce said washing liquid into the latter;

characterized in that it comprises, in sequence along said duct (105), between said first connector (101) and said discharge member (103):

- a first filtering member (108) configured to prevent the passage of mature cells and, instead, able to be crossed by adipocyte precursors and stem cells and by a stromal vascular component;

- a third connector (109) able to be coupled with a receiving member (110) configured to receive fluid from said duct (105);

- a second filtering member (111) configured to prevent the passage of precursors of vital adipocytes and vital stem cells and, instead, able to be crossed by blood and/or washing liquid and by a stromal vascular component;

wherein said filtering members (108, 111) are arranged, along said duct (105), between said first connector (101) and said second connector (107) so that, during use, a flow of washing liquid coming from said washing tank (106) and directed towards said containment member (102), passes through said filtering members (108, 111).

2. Device according to claim 1, wherein said first filtering member (108) is configured to prevent the passage of particles having dimensions not smaller than a first size chosen in a range of between 50 microns and 400 microns; wherein said second filtering member (111) is configured to prevent the passage of particles having dimensions not smaller than a second size chosen in a range of between 5 microns and 50 microns.

3. Device according to claim 1, wherein said first filtering member (108) is configured to prevent the passage of particles having dimensions not smaller than a first size chosen in a range of between 20 microns and 400 microns; wherein said second filtering member (111) is configured to prevent the passage of particles having dimensions not smaller than a second size chosen in a range of between 5 microns and 80 microns.

4. Device according to one of the preceding claims, wherein said washing tank (106) is connected to said duct (105) by means of a valve member (112) configured to allow the outflow from said washing tank (106) and prevent reflux into said washing tank (106).

5. Liposuction fat purification method for producing fat purified by means of a device according to one of the preceding claims, which comprises the following steps, in succession:

A. forcing a flow of aspirated material to pass, in succession, through said first filtering member (108), so as to filter a first fraction of said aspirated material, and said second filtering member (111), so as to filter a second fraction of aspirated material and discharge a residual fraction of said aspirated material, through said discharge member (103);

B. aspirating from said washing tank (106) a washing liquid so as to mix it with said first fraction and/or with said second fraction;

C. forcing a mixture of said washing liquid with said first fraction and/or with said second fraction through said filtering members (108, 111) so as to obtain a first purified fraction rich in vital mature cellular elements, a second purified fraction rich in precursors of vital adipocytes and vital stem cells, and a waste fraction; wherein said first purified fraction and said second purified fraction are respectively filtered by said first filtering member (108) and by said second filtering member (111), said waste fraction being forced through said discharge member (103);

D. repeating steps B and C cyclically;

wherein said method comprises, following said step D, the following steps in sequence: E. emulsifying said first purified fraction so as to obtain nanofat;

F. adding said nanofat to said second purified fraction so as to enrich said nanofat with said vital adipocyte precursors and said vital stem cells.