WO2020228710A1 - 离心管、检测系统及检测方法 - Google Patents

离心管、检测系统及检测方法 Download PDF

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Publication number
WO2020228710A1
WO2020228710A1 PCT/CN2020/089900 CN2020089900W WO2020228710A1 WO 2020228710 A1 WO2020228710 A1 WO 2020228710A1 CN 2020089900 W CN2020089900 W CN 2020089900W WO 2020228710 A1 WO2020228710 A1 WO 2020228710A1
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WIPO (PCT)
Prior art keywords
electrode
detection
centrifuge tube
extraction electrode
centrifuge
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PCT/CN2020/089900
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English (en)
French (fr)
Inventor
朱琳
Original Assignee
京东方科技集团股份有限公司
北京京东方技术开发有限公司
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Priority to US17/279,161 priority Critical patent/US11904320B2/en
Publication of WO2020228710A1 publication Critical patent/WO2020228710A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis

Definitions

  • the embodiment of the present disclosure relates to a centrifuge tube, a detection system and a detection method.
  • saliva component detection is mostly carried out in hospitals.
  • the quantitative detection of saliva component markers mainly relies on immunological and biochemical methods, such as culture method, PCR (Polymerase Chain Reaction, polymerase chain reaction), and enzyme-linked immunosorbent assay. And so on, it requires a dedicated person to perform multi-step operations, which is complicated.
  • At least one embodiment of the present disclosure provides a centrifuge tube, including:
  • Tube body including inner and outer surfaces
  • the second electrode group is on the outer surface of the tube body and is electrically connected to the first electrode group.
  • At least one embodiment of the present disclosure provides a detection system including a detection device and a centrifuge tube according to any embodiment of the present disclosure.
  • the detection device is configured to be signal-connected to the centrifuge tube to process the electrical signal from the sensor.
  • At least one embodiment of the present disclosure provides a detection method, including the following steps:
  • the electrical signal of the sensor is processed by the detection device to obtain the value of the target parameter of the analyte to be detected.
  • Fig. 1 is a schematic structural diagram of a centrifuge tube according to at least one embodiment of the present disclosure
  • Fig. 2 is a schematic structural diagram of a centrifuge tube according to at least one embodiment of the present disclosure
  • FIG. 3 is a schematic expanded view of the inner surface of a centrifuge tube provided by at least one embodiment of the present disclosure
  • FIG. 4 is a schematic expanded view of the outer surface of a centrifuge tube provided by at least one embodiment of the present disclosure
  • FIG. 5 is a schematic structural diagram of an electrochemical sensor in a centrifuge tube provided by at least one embodiment of the present disclosure
  • Fig. 6 is another schematic structural diagram of a centrifuge tube provided by at least one embodiment of the present disclosure.
  • Fig. 7 is a schematic block diagram of a detection system according to at least one embodiment of the present disclosure.
  • FIG. 8 is a schematic structural diagram of a detection system according to at least one embodiment of the present disclosure.
  • Fig. 9 is another schematic structural diagram of a detection system according to at least one embodiment of the present disclosure.
  • FIG. 10 is another schematic structural diagram of a detection system according to at least one embodiment of the present disclosure.
  • FIG. 11 is a schematic flowchart of an example of a detection method according to at least one embodiment of the present disclosure.
  • At least one embodiment of the present disclosure provides a centrifuge tube, a detection system, and a detection method.
  • the test substance is put into the centrifuge tube, the centrifuge tube is put into the centrifuge for centrifugation, the sensor contacts the supernatant in the centrifuge tube, and the detection device outputs analysis according to the electrical signal of the sensor
  • the operation is simple, and the problem of complicated operation of the existing detection method can be solved.
  • At least one embodiment of the present disclosure provides a centrifuge tube, which includes a tube body, a sensor, and a second electrode group.
  • the pipe body includes an inner surface and an outer surface.
  • the sensor is on the inner surface of the tube and includes a first electrode group.
  • the second electrode group is on the outer surface of the tube body and is electrically connected to the first electrode group.
  • Fig. 1 is a schematic structural diagram of a centrifuge tube provided by at least one embodiment of the present disclosure.
  • Fig. 3 is a schematic expanded view of the inner surface of a centrifuge tube provided by at least one embodiment of the present disclosure.
  • 4 is a schematic expanded view of the outer surface of a centrifuge tube provided by at least one embodiment of the disclosure.
  • FIG. 5 is another structural schematic diagram of the electrochemical sensor in the centrifuge tube provided by at least one embodiment of the disclosure.
  • a centrifuge tube according to at least one embodiment of the present disclosure includes a tube body 10, a sensor, a first extraction electrode 11, at least one second extraction electrode 12, and a third extraction electrode 13. .
  • the pipe body 10 includes an inner surface 101 and an outer surface 102.
  • the sensor is on the inner surface 101 of the tube body 10.
  • the sensor includes a reference electrode 21, a counter electrode 23 and at least one working electrode 22.
  • the first extraction electrode 11, the at least one second extraction electrode 12 and the third extraction electrode 13 are arranged at intervals on the outer surface 102 of the tube body 10.
  • the first extraction electrode 11 is electrically connected to the reference electrode 21, the at least one second extraction electrode 12 is electrically connected to the at least one working electrode 22, the third extraction electrode 13 is electrically connected to the counter electrode 23, and the at least one working electrode 22 is electrically connected to the at least one first electrode.
  • the two lead electrodes 12 correspond one to one.
  • the aforementioned sensor may be, for example, an electrochemical sensor or a biosensor, which is not limited in the embodiments of the present disclosure.
  • an electrochemical sensor will be used as an example to describe various embodiments of the present disclosure.
  • the reference electrode 21, the counter electrode 23, and the at least one working electrode 22 are only examples of the first electrode group, and the first extraction electrode 11, at least one second extraction electrode 12, and the third extraction electrode 13 are only examples of the second electrode group
  • the embodiment of the present disclosure does not limit this.
  • the reference electrode 21, the counter electrode 23, and the at least one working electrode 22 may directly contact the inner surface 101 of the tube body 10 or there may be other intermediate layers, which are not limited in the embodiment of the present disclosure.
  • the reference electrode 21, the counter electrode 23, and the at least one working electrode 22 can be connected to the inner surface 101 of the tube body 10 by means of brazing, laser welding, bonding or the like.
  • any one of the first extraction electrode 11, the second extraction electrode 12, and the third extraction electrode 13 may correspond to the reference electrode 21, the working electrode 22, and the counter electrode 23 through a via hole on the side wall of the tube body 10.
  • the electrodes are electrically connected.
  • the centrifuge tube according to at least one embodiment of the present disclosure may further include a lead, one end of the lead is electrically connected to any one of the reference electrode 21, the working electrode 22, and the counter electrode 23, and the other end of the lead is connected to the first lead Corresponding electrodes among the electrode 11, the second extraction electrode 12, and the third extraction electrode 13 are electrically connected.
  • the lead can extend from any one of the reference electrode 21, the working electrode 22 and the counter electrode 23 out of the centrifuge tube along the inner surface 101 of the tube body 10 of the centrifuge tube, and then bend in the opposite direction, and then follow the tube body of the centrifuge tube.
  • the outer surface 102 of 10 extends to the corresponding electrodes among the first extraction electrode 11, the second extraction electrode 12 and the third extraction electrode 13.
  • a centrifuge tube includes a first lead, a second lead, and a third lead.
  • the first lead includes a first portion 31a extending along the inner surface 101 of the tube body 10 and a second portion 31b extending along the outer surface 102 of the tube body 10.
  • the second lead includes a first portion 32 a extending along the inner surface 101 of the tube body 10 and a second portion 32 b extending along the outer surface 102 of the tube body 10.
  • the third lead includes a first portion 33 a extending along the inner surface 101 of the tube body 10 and a second portion 33 b extending along the outer surface 102 of the tube body 10.
  • the embodiment of the present disclosure does not limit the specific connection manner of the first extraction electrode and the reference electrode, as long as the first extraction electrode can extract the electrical signal of the reference electrode.
  • the embodiments of the present disclosure also do not limit the specific connection manner of the second extraction electrode and the working electrode, and the specific connection manner of the third extraction electrode and the counter electrode.
  • an electrochemical sensor can generate an electrical signal that is proportional to the concentration of the analyte to be detected. By processing the electrical signal, the concentration of the analyte to be detected can be obtained according to the size of the electrical signal. The sensor detects the concentration of the analyte, the detection speed is fast, the detection result is accurate, and the operation is simple, portable, and the detection cost is reduced.
  • the electrochemical sensor may be arranged closer to the opening of the tube body 10 relative to the bottom of the tube body 10.
  • the electrochemical sensor can be arranged on the upper half, upper third, upper quarter, etc. of the tube body 10, which is not limited in the embodiment of the present disclosure.
  • the first extraction electrode is electrically connected to the reference electrode
  • the first extraction electrode is electrically connected to the reference electrode
  • the second extraction electrode is electrically connected to the working electrode
  • the second extraction electrode is electrically connected to the working electrode
  • the third extraction electrode is electrically connected to the counter electrode. Connected, the third extraction electrode leads out the electrical signal of the counter electrode.
  • the first extraction electrode, the second extraction electrode and the third extraction electrode are arranged on the outer surface of the tube body to facilitate the extraction of the electrical signals generated by the electrochemical sensor on the inner surface of the tube body during the detection process for further processing, so as to obtain the detection result .
  • the first extraction electrode, the second extraction electrode and the third extraction electrode are arranged at intervals to avoid the interference of the signal between the reference electrode, the working electrode and the counter electrode, and improve the accuracy of the detection result.
  • the electrochemical sensor includes at least one working electrode.
  • multiple working electrodes When multiple working electrodes are provided, multiple analytes can be detected at the same time, which improves the detection efficiency.
  • There is a one-to-one correspondence between the working electrode and the second extraction electrode that is, one working electrode corresponds to one second extraction electrode.
  • there are multiple working electrodes there are also multiple second extraction electrodes. With the same number, the multiple second extraction electrodes can respectively extract the electrical signals of the multiple working electrodes, thereby achieving the purpose of detecting multiple analytes.
  • the first extraction electrode 11, the second extraction electrode 12, and the third extraction electrode 13 are all ring electrodes extending at least partly along the outer surface of the tube body 10 in the circumferential direction.
  • the ring electrode may be an exposed ring-shaped conductive material that is not covered by an insulating layer; or, the ring electrode may be a ring-shaped conductive material partially covered with an insulating layer.
  • the first extraction electrode 11, the second extraction electrode 12, and the third extraction electrode 13 are all shown as ring electrodes extending in a circumferential direction along the outer surface of the tube body 10.
  • the aforementioned conductive material may be, for example, metal, indium tin oxide, indium zinc oxide, etc., which are not limited in the embodiments of the present disclosure.
  • first extraction electrode 11, the second extraction electrode 12, and the third extraction electrode 13 are all ring electrodes extending along the outer surface of the tube body 10 in the circumferential direction
  • some of the first extraction electrode 11, the second extraction electrode 12, and the third extraction electrode 13 may be ring electrodes extending in the circumferential direction of the outer surface of the tube body 10, and the first extraction electrode 11.
  • the other electrodes of the second extraction electrode 12 and the third extraction electrode 13 adopt other shapes, which are not limited in the embodiment of the present disclosure.
  • the centrifuge tube is rotated to any angle, and the first extraction electrode
  • the electrodes, the second extraction electrode, and the third extraction electrode can all be connected to the detection device, which facilitates the connection of the first extraction electrode, the second extraction electrode, and the third extraction electrode with the detection device, thereby connecting the reference electrode, the working electrode and the counter electrode.
  • the electrical signal is transmitted to the detection device, and the electrical signal is further processed by the detection device to obtain the detection result, which improves the detection speed.
  • Fig. 2 is another schematic structural diagram of a centrifuge tube provided by at least one embodiment of the present disclosure.
  • the centrifuge tube may further include a pad or a spring pressure piece electrically connected to the ring electrode.
  • the outer wall of the tube body 10 is provided with a first pad or a first spring pressing piece 14, a second pad or a second spring pressing piece 15 and a third pad or a third spring pressing piece 16, the first pad or the second spring pressing piece at intervals.
  • a spring pressing piece 14 is electrically connected to the first lead electrode 11, the second pad or second spring pressing piece 15 is electrically connected to the second lead electrode 12, and the third pad or third spring pressing piece 16 is electrically connected to the third lead electrode 12 13 Electrical connection.
  • the first pad, the second pad, and the third pad are spaced apart on the sidewall of the tube body, which can improve the reliability of the connection and facilitate the connection of the first pad, the second pad and the
  • the third pad is connected to the detection device to transmit the electrical signals of the reference electrode, the working electrode and the counter electrode to the detection device, and the electrical signals are further processed by the detection device to obtain the detection result, which improves the detection speed.
  • the first spring pressing piece, the second spring pressing piece, and the third spring pressing piece can make the first lead electrode, the second lead electrode, and the third lead
  • the electrode and the detection device form a stable electrical connection, which avoids the problem of poor contact between some lead electrodes and the detection device affecting the detection result, and improves the stability and reliability of the detection result.
  • Fig. 6 is another schematic diagram of a centrifuge tube provided by at least one embodiment of the present disclosure.
  • the first extraction electrode 11, the second extraction electrode 12, and the third extraction electrode 13 can also be directly formed in the form of a pad or a spring pressure piece.
  • the connection of the first extraction electrode 11, the second extraction electrode 12, and the third extraction electrode 13 to the reference electrode 21, the working electrode 22, and the counter electrode 23 in FIG. 6 can be the same as those mentioned in other embodiments of the present disclosure. The same, this article will not repeat them.
  • first lead electrode 11, the second lead electrode 12, and the third lead electrode 13 in FIG. 6 are all in the form of a pad or a spring pressure piece, in other embodiments, the first lead electrode 11, the Part of the electrodes in the second extraction electrode 12 and the third extraction electrode 13 can be directly formed in the form of pads or spring pressing pieces, while the other electrodes in the first extraction electrode 11, the second extraction electrode 12 and the third extraction electrode 13 can be Other forms, such as ring electrodes, are not limited in the embodiments of the present disclosure.
  • the working electrode 22 is provided with a reactant or catalyst corresponding to the analyte to be detected, and the reactant or catalyst can chemically react with the analyte to be detected to generate an electrical signal.
  • the concentration of the analyte to be detected can be obtained according to the size of the electrical signal.
  • the electrochemical sensor includes at least one working electrode.
  • the electrochemical sensor includes multiple working electrodes, different reactants or catalysts can be arranged on each working electrode, and each working electrode is used to detect different analytes, so that multiple analytes can be detected simultaneously. The analyte is detected, which improves the detection efficiency.
  • the electrochemical sensor includes a working electrode 22, which can detect an analyte in the test object.
  • the electrochemical sensor includes two working electrodes 22, which can simultaneously detect two analytes in the test substance.
  • the working electrode 22 may be provided with any one of lactate oxidase, glucose oxidase, urate oxidase, and the like.
  • the object to be detected may be saliva, blood, urine, etc., which are not limited in the embodiments of the present disclosure.
  • the analyte may be any one of glucose, lactate, urea, and the like.
  • the concentration of lactate in saliva can be detected by lactate oxidase
  • the concentration of glucose in saliva can be detected by glucose oxidase
  • the concentration of glucose in saliva can be detected by urate oxidase.
  • any one of lactate oxidase, glucose oxidase, and urate oxidase can be set on the working electrode to detect the concentration of a corresponding analyte in saliva; for example, Lactic acid oxidase is set on the working electrode to detect the concentration of the corresponding analyte lactate in the saliva; glucose oxidase can be set on the working electrode to detect the concentration of glucose in the saliva; uric acid oxidation can also be set on the working electrode Enzymes to detect the concentration of urea in saliva.
  • any two of lactate oxidase, glucose oxidase, and urate oxidase can be set on the two working electrodes, so as to simultaneously detect the concentration of the two corresponding analytes in saliva , To improve detection efficiency.
  • lactate oxidase can be set on one working electrode and glucose oxidase can be set on the other working electrode to simultaneously detect the concentration of lactic acid and glucose in saliva; lactate oxidase can be set on one working electrode, Set urate oxidase on the other working electrode to simultaneously detect the concentration of lactic acid and urea in saliva; you can also set glucose oxidase on one working electrode and set urate oxidase on the other working electrode to detect saliva at the same time The concentration of glucose and urea.
  • the object to be detected is blood or urine is similar to the case where the object to be detected is saliva, so the detailed description of the case where the object to be detected is blood or urine is omitted here.
  • Fig. 7 is a schematic block diagram of a detection system provided by at least one embodiment of the present disclosure.
  • FIG. 8 is a schematic structural diagram of a detection system provided by at least one embodiment of the present disclosure.
  • FIG. 9 is a schematic diagram of another structure of a detection system provided by at least one embodiment of the present disclosure.
  • FIG. 10 is a schematic diagram of another structure of a detection system provided by at least one embodiment of the present disclosure.
  • the detection system 200 according to at least one embodiment of the present disclosure includes a centrifuge tube 210 and a detection device 220.
  • the centrifuge tube 210 may be the centrifuge tube described in any embodiment of the present disclosure.
  • the detection device 220 is configured to be in signal connection with the centrifuge tube 210 to process electrical signals from the sensor of the centrifuge tube 210.
  • the detection device processes the electrical signals from the electrochemical sensor of the centrifuge tube (for example, the reference electrode, the working electrode, and the counter electrode of the electrochemical sensor) to obtain the detection result, and the detection device and the centrifuge tube are arranged separately When the analyte in the centrifuge tube is centrifuged, the parts that work together with the centrifuge tube are reduced, thereby reducing the impact on the detection device, extending the service life of the detection device, and improving the accuracy of the detection result.
  • the electrochemical sensor of the centrifuge tube for example, the reference electrode, the working electrode, and the counter electrode of the electrochemical sensor
  • the detection system 200 further includes a centrifuge 40 and a centrifuge disk 240 rotatably connected with the centrifuge 40.
  • the centrifuge disc 240 is configured to fix the centrifuge tube 210.
  • the centrifuge 40 includes a side wall 41, and the detection device 220 is on the side wall 41.
  • the centrifuge 40 is used to perform the centrifugal treatment on the object to be detected in the centrifuge tube 210, the centrifuge tube 210 can be fixed on the centrifuge disk 240, and the side wall 41 of the centrifuge 40 can fix the detection device 220.
  • the test substance is put into the centrifuge tube, the centrifuge tube is fixed on the centrifuge disc, and the centrifuge performs the centrifugation treatment on the test substance. After the centrifugation process is completed, the centrifuge disc stops rotating and sets the centrifuge
  • the detection device on the side wall of the machine is electrically connected with the first, second, and third extraction electrodes of the centrifuge tube.
  • the detection device processes the electrical signals of the electrochemical sensor to obtain the detection results of the object to be detected. The operation is simple , To facilitate the detection of the object to be tested.
  • the detection device 220 includes a detection circuit 221, a first detection electrode 31, a second detection electrode 32, and a third detection electrode 33.
  • the first detection electrode 31 is configured to be electrically connected to the first extraction electrode 11
  • the second detection electrode 32 is configured to be electrically connected to the second extraction electrode 12
  • the third detection electrode 33 is configured to be electrically connected to the third extraction electrode 13
  • a detection electrode 31, a second detection electrode 32 and a third detection electrode 33 are signally connected to the detection circuit 221.
  • the first detection electrode 31 is electrically connected to the first extraction electrode 11 so as to transmit the electrical signal of the reference electrode to the detection circuit
  • the second detection electrode 32 and the second extraction electrode 12 are in one-to-one electrical correspondence.
  • the third detection electrode 33 is electrically connected to the third extraction electrode 13, thereby transmitting the electrical signal of the counter electrode to the detection circuit, and the detection circuit performs the electrical signal of the electrochemical sensor. Processing to obtain the test result of the object to be tested.
  • the position of the first detection electrode 31 corresponds to the position of the first extraction electrode 11
  • the position of the second detection electrode 32 corresponds to the position of the second extraction electrode 12
  • the position of the third detection electrode 33 corresponds to the position of the third extraction electrode.
  • the position of 13 corresponds.
  • the detection system of the embodiment of the present disclosure it is convenient to connect the first detection electrode with the first extraction electrode, connect the second detection electrode with the second extraction electrode, and connect the third detection electrode with the third extraction electrode. , Improve the detection speed and detection efficiency.
  • the detection system 200 further includes a first elastic member 34, a second elastic member 35 and a third elastic member 36.
  • first detection electrode 31 is connected to the side wall 41 of the centrifuge 40 through the first elastic member 34
  • second detection electrode 32 is connected to the side wall 41 of the centrifuge 40 through the second elastic member 35
  • third detection electrode 33 passes through
  • the third elastic member 36 is connected to the side wall 41 of the centrifuge 40.
  • the detection system 200 further includes an electromagnet 42.
  • the electromagnet 42 is fixedly connected to the centrifuge 40, and is configured to attract the first detection electrode 31, the second detection electrode 32, and the third detection electrode 33 toward the side wall 41 when energized.
  • the detection system 200 may include a plurality of electromagnets for attracting the first detection toward the side wall 41, respectively.
  • the electrode 31, the second detection electrode 32 and the third detection electrode 33 are shown, and the third detection electrode 33.
  • the first detection electrode 31, the second detection electrode 32, and the third detection electrode 33 are located on the first pad or the first spring pressing piece 14, the second pad or the second pad, respectively.
  • the projection on the spring pressing piece 15 and the third pad or the third spring pressing piece 16 can be the same as the first spring pressing piece 14, the second land or the second spring pressing piece 15 and the third land or the third spring pressing piece. 16 basically coincides.
  • the first detection electrode 31, the second detection electrode 32, and the third detection electrode 33 are located on the first pad or the first spring pressing piece 14, the second pad or the second pad, respectively.
  • the projections on the second spring pressing piece 15 and the third land or the third spring pressing piece 16 can fall into the first land or the first spring pressing piece 14, the second land or the second spring pressing piece 15 and the third welding The inside of the disc or the third spring press piece 16 to save materials.
  • the electromagnet when the centrifuge is performing centrifugal treatment on the object to be tested, the electromagnet is energized and attracts the first detection electrode, the second detection electrode, and the third detection electrode.
  • the first elastic member and the second elastic The first detection electrode, the second detection electrode, and the third detection electrode are not in contact with the centrifuge tube, that is, the first detection electrode is not in contact with the first extraction electrode, the first pad or The first spring pressure piece contacts, the second detection electrode does not contact the second extraction electrode, the second pad or the second spring pressure piece, and the third detection electrode does not contact the third extraction electrode, the third pad or the third spring Press sheet contact; after the centrifugal treatment is completed, the electromagnet is powered off.
  • the centrifuge tube moves, and the first detection electrode, the second detection electrode, and the third detection electrode are close to the outer surface of the centrifuge tube, so that the first detection electrode is in contact with the first extraction electrode, the first pad or the first spring pressure piece ,
  • the second detection electrode is in contact with the second pad of the second extraction electrode or the second spring pressing piece, and the third detection electrode is in contact with the third extraction electrode, the third pad or the third spring pressing piece, thereby connecting the electrochemical sensor
  • the electrical signal is transmitted to the detection circuit, so that the detection circuit processes the electrical signal, thereby obtaining the detection result, and the operation is simple.
  • At least one embodiment of the present disclosure provides a detection method, which is applicable to the detection system described in any embodiment of the present disclosure.
  • the detection method includes:
  • the electrical signal of the sensor is processed by the detection device to obtain the value of the target parameter of the analyte to be detected.
  • the second electrode group includes a first extraction electrode, at least one second extraction electrode, and a third extraction electrode
  • electrically connecting the second electrode group to the detection device may include:
  • the detection device does not receive the electrical signal of the sensor
  • FIG. 11 is a schematic flowchart of an example of a detection method provided by at least one embodiment of the present disclosure.
  • the detection method according to at least one embodiment of the present disclosure includes the following steps:
  • the working electrode of the electrochemical sensor is in contact with the supernatant liquid in the centrifuge tube, and the first extraction electrode, the second extraction electrode and the third extraction electrode are respectively electrically connected to the detection device;
  • the detection device processes the electrical signal of the electrochemical sensor to obtain the concentration of the analyte to be detected.
  • the object to be detected is put into the centrifuge tube, the centrifuge performs the centrifugal treatment of the object to be detected, and the electrochemical sensor detects the supernatant obtained after the centrifugal treatment, so that the detection result is more accurate, and the detection device passes
  • the first extraction electrode, the second extraction electrode and the third extraction electrode obtain the electrical signal of the electrochemical sensor.
  • the detection device processes the electrical signal to obtain the target parameter values of the analyte in the test, such as concentration, reaction time, etc.
  • the operation is simple , Reduce the cost of testing and reduce the reagents consumed in the testing process.
  • electrically connecting the first extraction electrode, the second extraction electrode and the third extraction electrode to the detection device respectively includes: pressing the detection device against the outer surface of the centrifuge tube, and detecting whether the detection device receives the electrical signal of the electrochemical sensor If it is received, the electrical connection is completed. If it is not received, the centrifuge tube is rotated until the detection device receives the electrical signal of the electrochemical sensor.
  • the first detection electrode, the second detection electrode, and the third detection electrode of the detection device bear against the centrifugal tube
  • the first detection electrode, the second detection electrode and the third detection electrode of the detection device can be contacted with the first extraction electrode, the second extraction electrode and the third extraction electrode, so that the detection device can Receive the electrical signal of the electrochemical sensor.
  • the outer surface of the tube body is provided with a first pad, a second pad, and a third pad at intervals, or the outer surface of the tube body is provided with a first spring pressing piece, a second spring pressing piece and a third spring pressing piece at intervals.
  • the centrifuge tube needs to be rotated, but not only the centrifuge tube Rotation of degrees/step, 5 degrees/step, 10/step, 15 degrees/step, etc., adjust the position so that the detection device and the first pad or the first spring pressing piece, the second pad or the second spring pressing piece and The third pad or the third spring pressure piece is in contact, so that the detection device can receive the electrical signals of the reference electrode, the working electrode and the counter electrode of the electrochemical sensor.

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Abstract

一种离心管、检测系统及检测方法。该离心管包括管体（10）、传感器和第二电极组。管体（10）包括内表面（101）和外表面（102）。传感器在管体（10）的内表面（101），并包括第一电极组。第二电极组在管体（10）的外表面（102）并与第一电极组电连接。

Description

离心管、检测系统及检测方法
相关申请的交叉引用
本申请要求于2019年5月13日递交的第201910395030.X号中国专利申请的优先权，在此出于所有目的全文引用上述中国专利申请公开的内容以作为本申请的一部分。
技术领域
本公开的实施例涉及一种离心管、检测系统及检测方法。
背景技术
传统的唾液成分检测大都在医院进行临床检测，唾液成分标志物定量检测主要依靠免疫学和生物化学的方式，诸如培养法、PCR(Polymerase Chain Reaction，聚合酶链式反应)、酶联免疫吸附法等，需要专人进行多步骤操作，操作复杂。
发明内容
本公开至少一个实施例提供了一种离心管，包括：
管体，包括内表面和外表面；
传感器，在所述管体的所述内表面，并包括第一电极组；以及
第二电极组，在所述管体的所述外表面并与所述第一电极组电连接。
本公开至少一个实施例提供了一种检测系统，包括检测装置以及根据本公开任一实施例的离心管。检测装置配置为与所述离心管信号连接，以处理来自所述传感器的电信号。
本公开至少一个实施例提供了一种检测方法，包括以下步骤：
将待检测物放入所述离心管，进行离心处理，获得上层清液和沉淀物并使得所述传感器的所述第一电极组与所述离心管内的所述上层清液接触；
将所述第二电极组与所述检测装置电连接；以及
通过所述检测装置对所述传感器的电信号进行处理，获取所述待检测物中待分析物的目标参数的值。
附图说明
为了更清楚地说明本公开实施例的技术方案，下面将对实施例的附图作简单地介绍，显而易见地，下面描述中的附图仅仅涉及本公开的一些实施例，而非对本公开的限制。
图1为根据本公开至少一个实施例的离心管的结构示意图；
图2为根据本公开至少一个实施例的离心管的结构示意图；
图3为本公开至少一个实施例提供的离心管的内表面的示意性展开图；
图4为本公开至少一个实施例提供的离心管的外表面的示意性展开图；
图5为本公开至少一个实施例提供的离心管中的电化学传感器的结构示意图；
图6为本公开至少一个实施例提供的离心管的另一结构示意图；
图7为根据本公开至少一个实施例的检测系统的示意性框图；
图8为根据本公开至少一个实施例的检测系统的一种结构示意图；
图9为根据本公开至少一个实施例的检测系统的另一结构示意图；
图10为根据本公开至少一个实施例的检测系统的另一结构示意图；
图11为根据本公开至少一个实施例的检测方法的示例的流程示意图。
具体实施方式
为使本公开实施例的目的、技术方案和优点更加清楚，下面将结合本公开实施例的附图，对本公开实施例的技术方案进行清楚、完整地描述。显然，所描述的实施例是本公开的一部分实施例，而不是全 部的实施例。基于所描述的本公开的实施例，本领域普通技术人员在无需创造性劳动的前提下所获得的所有其他实施例，都属于本公开保护的范围。
除非另作定义，此处使用的技术术语或者科学术语应当为本公开所属领域内具有一般技能的人士所理解的通常意义。本公开中使用的“第一”、“第二”以及类似的词语并不表示任何顺序、数量或者重要性，而只是用来区分不同的组成部分。同样，“包括”或者“包含”等类似的词语意指出现该词前面的元件或者物件涵盖出现在该词后面列举的元件或者物件及其等同，而不排除其他元件或者物件。“连接”或者“相连”等类似的词语并非限定于物理的或者机械的连接，而是可以包括电性的连接，不管是直接的还是间接的。“上”、“下”、“左”、“右”等仅用于表示相对位置关系，当被描述对象的绝对位置改变后，则该相对位置关系也可能相应地改变。
本公开至少一个实施例提供一种离心管、检测系统和检测方法。根据本公开实施例提供的技术方案，将待检测物放入离心管中，将离心管放入离心机进行离心处理，传感器接触离心管中的上层清液，检测装置根据传感器的电信号输出分析结果，操作简单，能够解决现有的检测方法操作复杂的问题。
本公开至少一个实施例提供了一种离心管，其包括：管体、传感器和第二电极组。管体包括内表面和外表面。传感器在管体的内表面，并包括第一电极组。第二电极组在管体的外表面上并与第一电极组电连接。
图1为本公开至少一个实施例提供的离心管的一个结构示意图。图3为本公开至少一个实施例提供的离心管的内表面的示意性展开图。图4为本公开至少一个实施例提供的离心管的外表面的示意性展开图。图5为本公开至少一个实施例提供的离心管中的电化学传感器的另一个结构示意图。请参考图1、图3、图4和图5，根据本公开至少一个实施例的离心管包括管体10、传感器、第一引出电极11、至少一个第二引出电极12和第三引出电极13。
管体10包括内表面101和外表面102。传感器在管体10的内表 面101。传感器包括参考电极21、对电极23和至少一个工作电极22。第一引出电极11、至少一个第二引出电极12和第三引出电极13在管体10的外表面102上间隔设置。第一引出电极11与参考电极21电连接，至少一个第二引出电极12与至少一个工作电极22电连接，第三引出电极13与对电极23电极电连接，至少一个工作电极22与至少一个第二引出电极12一一对应。
上述的传感器例如可以是电化学传感器或生物传感器等，本公开的实施例对此不作限制。在本公开中将以电化学传感器为例来描述本公开的各实施例。
参考电极21、对电极23和至少一个工作电极22仅是第一电极组的示例，以及第一引出电极11、至少一个第二引出电极12和第三引出电极13仅是第二电极组的示例，本公开的实施例对此不作限制。
参考电极21、对电极23和至少一个工作电极22可与管体10的内表面101直接接触或还可存在其他中间层，本公开的实施例对此不作限制。例如，参考电极21、对电极23和至少一个工作电极22与管体10的内表面101可通过钎焊、激光焊接、粘接等方式进行连接。
例如，第一引出电极11、第二引出电极12和第三引出电极13中的任一个电极可以经由管体10的侧壁上的过孔与参考电极21、工作电极22和对电极23中对应的电极电连接。
例如，根据本公开至少一个实施例的离心管还可包括引线，该引线的一端与参考电极21、工作电极22和对电极23中的任一个电极电连接，该引线的另一端与第一引出电极11、第二引出电极12和第三引出电极13中的对应的电极电连接。
该引线例如可从参考电极21、工作电极22和对电极23中的任一个电极沿离心管的管体10的内表面101延伸出离心管，然后反向弯折，再沿离心管的管体10的外表面102延伸至第一引出电极11、第二引出电极12和第三引出电极13中的对应的电极。
例如，参照3和图4，根据本公开至少一个实施例的离心管包括第一引线、第二引线和第三引线。第一引线包括沿管体10的内表面101延伸的第一部分31a和沿管体10的外表面102延伸的第二部分 31b。第二引线包括沿管体10的内表面101延伸的第一部分32a和沿管体10的外表面102延伸的第二部分32b。第三引线包括沿管体10的内表面101延伸的第一部分33a和沿管体10的外表面102延伸的第二部分33b。
需要说明的是，本公开的实施例对第一引出电极和参考电极的具体连接方式不作限制，只要第一引出电极可以将参考电极的电信号引出即可。类似地，本公开的实施例对第二引出电极和工作电极的具体连接方式以及第三引出电极和对电极的具体连接方式也不作限制。
在本公开的实施例中，通常将待检测物放入离心管内进行离心处理后，沉淀物会在离心管的管体的底部，上层清液会在沉底物上方，在离心管的管体的内表面贴合电化学传感器，使得电化学传感器能够与上层清液接触并对其进行检测，减少沉淀物对检测结果的干扰，提高检测准确性。例如，电化学传感器能够产生与待检测物中待分析物浓度成正比的电信号，通过对电信号进行处理，能够根据电信号的大小等获得待检测物中待分析物的浓度，通过电化学传感器来检测待分析物的浓度，检测速度快，检测结果准确，同时，操作简单，便携，降低了检测成本。
如图3所示，在一些实施例中，电化学传感器可设置成相对于管体10的底部，离管体10的开口更近。例如，电化学传感器可设置在管体10的上半部分、上三分之一、上四分之一等，本公开的实施例对此不作限制。通过将电化学传感器设置成相对于管体10的底部离管体10的开口更近，可使得电化学传感器仅与上层清液接触，而不与沉淀物接触，减少沉淀物对检测结果的干扰，提高检测准确性。
第一引出电极与参考电极电连接，第一引出电极引出参考电极的电信号，第二引出电极与工作电极电连接，第二引出电极引出工作电极的电信号，第三引出电极与对电极电连接，第三引出电极引出对电极的电信号。第一引出电极、第二引出电极和第三引出电极设置在管体的外表面，便于将设置在管体内表面的电化学传感器在检测过程中产生的电信号引出进行进一步处理，从而获取检测结果。第一引出电极、第二引出电极和第三引出电极间隔设置，避免参考电极、工作电 极和对电极之间的信号产生干扰，提高了检测结果的准确性。
电化学传感器包括至少一个工作电极，当设置多个工作电极时，能够同时检测多个待分析物，提高检测效率。工作电极与第二引出电极一一对应，也就是一个工作电极对应一个第二引出电极，当存在多个工作电极时，也存在多个第二引出电极，第二引出电极的数量与工作电极的数量相同，多个第二引出电极能够将多个工作电极的电信号分别引出，从而实现检测多个待分析物的目的。
例如，第一引出电极11、第二引出电极12和第三引出电极13均为至少部分沿着管体10的外表面周向延伸的环形电极。在一些实施例中，该环形电极可以是没有被绝缘层覆盖的、裸露的环形导电材料；或者，该环形电极可以是部分覆盖有绝缘层的环形导电材料。例如，在图1中，第一引出电极11、第二引出电极12和第三引出电极13均示出为沿着管体10的外表面周向延伸一圈的环形电极，然而应理解，本公开的实施例并不限于此。上述的导电材料例如可以是金属、氧化铟锡、氧化铟锌等，本公开的实施例对此不作限制。
此外，还应理解虽然在本申请的各附图中，第一引出电极11、第二引出电极12和第三引出电极13均为沿着管体10的外表面周向延伸的环形电极，然而在其他实施例中，可以是第一引出电极11、第二引出电极12和第三引出电极13中的部分电极为沿着管体10的外表面周向延伸的环形电极，而第一引出电极11、第二引出电极12和第三引出电极13中的其他电极采用其他的形状，本公开的实施例对此不作限制。
在本公开的实施例中，例如，当第一引出电极、第二引出电极和第三引出电极均为没有被绝缘层覆盖的、裸露的环形电极时，离心管转动至任意角度，第一引出电极、第二引出电极和第三引出电极均能与检测装置对接，便于将第一引出电极、第二引出电极和第三引出电极与检测装置进行对接，从而将参考电极、工作电极和对电极的电信号传输到检测装置中，通过检测装置对电信号进行进一步处理，获得检测结果，提高了检测速度。图2为本公开至少一个实施例提供的离心管的另一个结构示意图。参考图2，根据本公开至少一个实施例的 离心管还可包括与环形电极电连接的焊盘或弹簧压片。管体10外侧壁间隔设置有第一焊盘或第一弹簧压片14、第二焊盘或第二弹簧压片15和第三焊盘或第三弹簧压片16，第一焊盘或第一弹簧压片14与第一引出电极11电连接，第二焊盘或第二弹簧压片15与第二引出电极12电连接，第三焊盘或第三弹簧压片16与第三引出电极13电连接。
在本公开的实施例中，在管体外侧壁间隔设置有第一焊盘、第二焊盘和第三焊盘，能提高连接的可靠性，便于将第一焊盘、第二焊盘和第三焊盘与检测装置进行对接，从而将参考电极、工作电极和对电极的电信号传输到检测装置中，通过检测装置对电信号进行进一步处理，获得检测结果，提高了检测速度。
在本公开的实施例中，在检测装置与离心管进行对接时，第一弹簧压片、第二弹簧压片和第三弹簧压片能够使得第一引出电极、第二引出电极和第三引出电极和检测装置形成稳定的电连接，避免出现部分引出电极与检测装置接触不良影响检测结果的问题，提高了检测结果的稳定性和可靠性。
图6为本公开至少一个实施例提供的离心管的另一示意图。如图6所示，第一引出电极11、第二引出电极12和第三引出电极13还可直接形成为焊盘或弹簧压片的形式。图6中的第一引出电极11、第二引出电极12和第三引出电极13与参考电极21、工作电极22和对电极23的连接方式可与本公开的其他实施例中提到的连接方式相同，本文将不再赘述。
应理解，虽然在图6中第一引出电极11、第二引出电极12和第三引出电极13均采用焊盘或弹簧压片的形式，然而在其他实施例中，第一引出电极11、第二引出电极12和第三引出电极13中的部分电极可直接形成为焊盘或弹簧压片的形式，而第一引出电极11、第二引出电极12和第三引出电极13中的其他电极可采用其他的形式，例如环形电极，本公开的实施例对此不作限制。
例如，工作电极22上设置有与待检测物中待分析物对应的反应物或催化剂，该反应物或催化剂可以与待检测物中待分析物发生化学反应以产生电信号，通过对该电信号进行处理，能够根据电信号的大小 等获得待检测物中待分析物的浓度。
在本公开的实施例中，电化学传感器包括至少一个工作电极。在电化学传感器包括多个工作电极的情况下，可以在各个工作电极上设置不同的反应物或催化剂，各工作电极用于检测不同的待分析物，从而能够同时对待检测物中的多个待分析物进行检测，提高了检测效率。
例如，在图3中，电化学传感器包括一个工作电极22，可以对检测物中的一种待分析物进行检测。例如，在图5中，电化学传感器包括两个工作电极22，可以同时对检测物中的两种待分析物进行检测。例如，工作电极22上可以设置有乳酸氧化酶、葡萄糖氧化酶、尿酸氧化酶等中任意一种。
例如，待检测物可以为唾液、血液和尿液等，本公开的实施例对此不做限制。
例如，待分析物可以为葡萄糖、乳酸盐和尿素等中的任意一种。
在本公开的实施例中，以待检测物为唾液为例，可以通过乳酸氧化酶来检测唾液中乳酸盐的浓度，可以通过葡萄糖氧化酶来检测唾液中葡萄糖的浓度，可以通过尿酸氧化酶来检测唾液中尿素的浓度。当电化学传感器包括一个工作电极时，可以在工作电极上设置乳酸氧化酶、葡萄糖氧化酶、尿酸氧化酶中任意一种，从而检测唾液中对应的一种待分析物的浓度；例如，可以在工作电极上设置乳酸氧化酶，以检测唾液中对应的待分析物乳酸盐的浓度；可以在工作电极上设置葡萄糖氧化酶，以检测唾液中葡萄糖的浓度；还可以在工作电极上设置尿酸氧化酶，以检测唾液中尿素的浓度。当电化学传感器包括两个工作电极时，可以分别在两个工作电极上设置乳酸氧化酶、葡萄糖氧化酶、尿酸氧化酶中任意两种，从而同时检测唾液中对应的两种待分析物的浓度，提高检测效率例如，可以在一个工作电极上设置乳酸氧化酶，在另一个工作电极上设置葡萄糖氧化酶，以同时检测唾液中乳酸和葡萄糖的浓度；可以在一个工作电极上设置乳酸氧化酶，在另一个工作电极上设置尿酸氧化酶，以同时检测唾液中乳酸和尿素的浓度；还可以在一个工作电极上设置葡萄糖氧化酶，在另一个工作电极上设置尿酸氧化酶，以同时检测唾液中葡萄糖和尿素的浓度。
需要说明的是，待检测物为血液或尿液的情形与待检测物为唾液的情形类似，因此这里省略了对待检测物为血液或尿液的情形的详细描述。
图7为本公开至少一个实施例提供的检测系统的示意性框图。图8为本公开至少一个实施例提供的检测系统的一种结构示意图。图9为本公开至少一个实施例提供的检测系统的另一种结构示意图。图10为本公开至少一个实施例提供的检测系统的另一种结构示意图。参考图7-图9，根据本公开至少一个实施例的检测系统200包括离心管210和检测装置220。离心管210可以是本公开任一实施例中描述的离心管。
检测装置220配置为与离心管210信号连接，以处理来自离心管210的传感器的电信号。
在本公开的实施例中，检测装置处理来自离心管的电化学传感器(例如电化学传感器的参考电极、工作电极和对电极)的电信号，从而获得检测结果，将检测装置和离心管分开设置，在对离心管内的待分析物进行离心处理时，减少与离心管共同动作的部件，从而减少对检测装置的影响，延长检测装置的使用寿命，提高检测结果的准确性。
例如，检测系统200还包括离心机40和与离心机40转动连接的离心盘240。
离心盘240配置为固定离心管210。离心机40包括侧壁41，检测装置220在侧壁41上。当使用离心机40对离心管210中的待检测物进行离心处理时，可以将离心管210固定在离心盘240上，离心机40的侧壁41固定检测装置220。
在本公开的实施例中，将待检测物放入离心管内，将离心管固定在离心盘上，离心机对待检测物进行离心处理，在离心处理完成后，离心盘停止转动，将设置在离心机的侧壁的检测装置与离心管的第一引出电极、第二引出电极和第三引出电极电连接，检测装置对电化学传感器的电信号进行处理，获得对待检测物的检测结果，操作简单，便于对待检测物进行检测。
例如，检测装置220包括检测电路221、第一检测电极31、第二 检测电极32以及第三检测电极33。第一检测电极31配置为与第一引出电极11电连接，第二检测电极32配置为与第二引出电极12电连接，第三检测电极33配置为与第三引出电极13电连接，并且第一检测电极31、第二检测电极32和第三检测电极33与检测电路221信号连接。
在本公开的实施例中，第一检测电极31与第一引出电极11电连接，从而将参考电极的电信号传输至检测电路，第二检测电极32与第二引出电极12一一对应地电连接，从而将工作电极的电信号传输至检测电路，第三检测电极33与第三引出电极13电连接，从而将对电极的电信号传输至检测电路，检测电路对电化学传感器的电信号进行处理，获得对待检测物的检测结果。
例如，第一检测电极31的位置与第一引出电极11的位置对应，第二检测电极32的位置与第二引出电极12的位置一一对应，第三检测电极33的位置与第三引出电极13的位置对应。
在本公开的实施例的检测系统中，便于将第一检测电极与第一引出电极进行对接，将第二检测电极与第二引出电极进行对接，将第三检测电极与第三引出电极进行对接，提高了检测速度和检测效率。
如图9和图10所示，在一些实施例中，检测系统200还包括第一弹性件34、第二弹性件35和第三弹性件36。例如，第一检测电极31通过第一弹性件34与离心机40的侧壁41连接，第二检测电极32通过第二弹性件35与离心机40的侧壁41连接，第三检测电极33通过第三弹性件36与离心机40的侧壁41连接。
如图9和图10所示，在一些实施例中，检测系统200还包括电磁铁42。电磁铁42与离心机40固定连接，并配置为在通电的情况下朝向侧壁41吸引第一检测电极31、第二检测电极32以及第三检测电极33。虽然在图9和图10中，仅示出了一个电磁铁42，然而应理解，在其他实施例中，检测系统200可包括多个电磁铁，以分别用于朝向侧壁41吸引第一检测电极31、第二检测电极32以及第三检测电极33。
如图9所示，在一些实施例中，第一检测电极31、第二检测电极32以及第三检测电极33分别在第一焊盘或第一弹簧压片14、第二焊盘或第二弹簧压片15和第三焊盘或第三弹簧压片16上的投影可与第 一弹簧压片14、第二焊盘或第二弹簧压片15和第三焊盘或第三弹簧压片16基本重合。如图10所示，在另一些实施例中，第一检测电极31、第二检测电极32以及第三检测电极33分别在第一焊盘或第一弹簧压片14、第二焊盘或第二弹簧压片15和第三焊盘或第三弹簧压片16上的投影可落入第一焊盘或第一弹簧压片14、第二焊盘或第二弹簧压片15和第三焊盘或第三弹簧压片16的内部，以节省物料。
在本公开的实施例中，在离心机对待检测物进行离心处理时，电磁铁通电并吸引第一检测电极、第二检测电极和第三检测电极，此时，第一弹性件、第二弹性件和第三弹性件处于压缩状态，第一检测电极、第二检测电极和第三检测电极不与离心管接触，也就是说，第一检测电极不与第一引出电极、第一焊盘或第一弹簧压片接触，第二检测电极不与第二引出电极、第二焊盘或第二弹簧压片接触，并且第三检测电极不与第三引出电极、第三焊盘或第三弹簧压片接触；在离心处理完成后，电磁铁断电，在第一弹性件、第二弹性件和第三弹性件的弹力作用下，第一检测电极、第二检测电极和第三检测电极向离心管运动，并且第一检测电极、第二检测电极和第三检测电极紧贴离心管的外表面，从而使得第一检测电极与第一引出电极、第一焊盘或第一弹簧压片接触，第二检测电极与第二引出电极第二焊盘或第二弹簧压片接触接触，第三检测电极与第三引出电极、第三焊盘或第三弹簧压片接触，从而将电化学传感器的电信号传输至检测电路，使得检测电路对电信号进行处理，从而获得检测结果，操作简单。
本公开的至少一个实施例提供一种检测方法，该检测方法适用于本公开任一实施例中描述的检测系统。该检测方法包括：
将待检测物放入离心管，进行离心处理，获得上层清液和沉淀物并使得传感器的第一电极组与离心管内的上层清液接触；
将第二电极组与检测装置电连接；以及
通过检测装置对传感器的电信号进行处理，获取待检测物中待分析物的目标参数的值。
例如，在第二电极组包括第一引出电极、至少一个第二引出电极和第三引出电极的情况下，将第二电极组与检测装置电连接可包括：
将检测装置顶住离心管的外表面；
检测出检测装置没有接收到传感器的电信号；
转动离心管，使得检测装置与第一引出电极、至少一个第二引出电极和第三引出电极信号连接。
图11为本公开至少一个实施例提供的检测方法的示例的示意流程图。参考图11，根据本公开至少一个实施例的检测方法，包括以下步骤：
将待检测物放入离心管，进行离心处理，获得上层清液和沉淀；
电化学传感器的工作电极与离心管内的上层清液接触，将第一引出电极、第二引出电极和第三引出电极分别与检测装置电连接；
检测装置对电化学传感器的电信号进行处理，获取待检测物中待分析物的浓度。
在本公开的实施例中，将待检测物放入离心管内，离心机对待检测物进行离心处理，电化学传感器对离心处理后获得的上层清液进行检测，使得检测结果更加准确，检测装置通过第一引出电极、第二引出电极和第三引出电极获取电化学传感器的电信号，检测装置对电信号进行处理获得检测物中待分析物的诸如浓度、反应时间等目标参数的值，操作简单，减少了检测费用，减少了检测过程中消耗的试剂。
例如，将第一引出电极、第二引出电极和第三引出电极分别与检测装置电连接，包括：将检测装置顶住离心管的外表面，并检测检测装置是否接收到电化学传感器的电信号，若接收到，则完成电连接，若没有接收到，则转动离心管，直至检测装置接收到电化学传感器的电信号。
在本公开的实施例中，在第一引出电极、第二引出电极和第三引出电极为环形电极时，检测装置的第一检测电极、第二检测电极和第三检测电极顶住离心管的外表面，不需要转动离心管，就能够使得检测装置的第一检测电极、第二检测电极和第三检测电极与第一引出电极、第二引出电极和第三引出电极接触，从而检测装置能够接收到电化学传感器的电信号。在管体的外表面间隔设置有第一焊盘、第二焊盘和第三焊盘，或者，管体的外表面间隔设置有第一弹簧压片、第二 弹簧压片和第三弹簧压片时，在检测装置的第一检测电极、第二检测电极和第三检测电极顶住离心管的外表面后，在检测装置的第一检测电极、第二检测电极和第三检测电极未与第一焊盘或第一弹簧压片、第二焊盘或第二弹簧压片以及第三焊盘或第三弹簧压片接触时，需要转动离心管，可以但不仅仅为将离心管进行1度/步、5度/步、10/步、15度/步等的旋转，调整位置，使得检测装置与第一焊盘或第一弹簧压片、第二焊盘或第二弹簧压片以及第三焊盘或第三弹簧压片接触，从而检测装置能够接收到电化学传感器的参考电极、工作电极和对电极的电信号。
虽然上文中已经用一般性说明及具体实施方式，对本公开作了详尽的描述，但在本公开实施例基础上，可以对之作一些修改或改进，这对本领域技术人员而言是显而易见的。因此，在不偏离本公开精神的基础上所做的这些修改或改进，均属于本公开要求保护的范围。

Claims (20)

  1. 一种离心管，包括：
    管体，包括内表面和外表面；
    传感器，在所述管体的所述内表面，并包括第一电极组；以及
    第二电极组，在所述管体的所述外表面并与所述第一电极组电连接。
  2. 根据权利要求1所述的离心管，其中，
    所述传感器包括电化学传感器，并且所述第一电极组包括至少一个参考电极、至少一个对电极和至少一个工作电极，
    所述第二电极组包括至少一个第一引出电极、至少一个第二引出电极和至少一个第三引出电极，
    所述至少一个第一引出电极、所述至少一个第二引出电极和所述至少一个第三引出电极在所述管体的所述外表面上间隔设置，以及
    所述至少一个第一引出电极与所述至少一个参考电极一一对应地电连接，所述至少一个第二引出电极与所述至少一个工作电极一一对应地电连接，所述第三引出电极与所述至少一个对电极一一对应地电连接。
  3. 根据权利要求2所述的离心管，其中，所述至少一个第一引出电极、所述至少一个第二引出电极和所述至少一个第三引出电极中至少之一为至少部分沿着所述管体的所述外表面周向延伸的环形电极。
  4. 根据权利要求3所述的离心管，其中，所述环形电极为部分覆盖有绝缘层的环形导电材料。
  5. 根据权利要求2-4中任一项所述的离心管，还包括在所述管体的所述外表面间隔设置的至少一个第一焊盘、至少一个第二焊盘和至少一个第三焊盘，
    其中，所述至少一个第一焊盘与所述至少一个第一引出电极一一对应地电连接，所述至少一个第二焊盘与所述至少一个第二引出电极一一对应地电连接，所述至少一个第三焊盘与所述至少一个第三引出电极一一对应地电连接。
  6. 根据权利要求2-4中任一项所述的离心管，还包括在所述管体的所述外表面间隔设置的至少一个第一弹簧压片、至少一个第二弹簧压片和 至少一个第三弹簧压片，
    其中，所述至少一个第一弹簧压片与所述至少一个第一引出电极一一对应地电连接，所述至少一个第二弹簧压片与所述至少一个第二引出电极一一对应地电连接，所述至少一个第三弹簧压片与所述至少一个第三引出电极一一对应地电连接。
  7. 根据权利要求2-6中任一项所述的离心管，其中，所述至少一个工作电极上设置有与待检测物中待分析物对应的反应物或催化剂，所述电化学传感器包括至少两个所述工作电极，各所述工作电极用于检测所述待检测物中不同的所述待分析物。
  8. 根据权利要求7所述的离心管，其中，所述反应物或催化剂包括选自由乳酸氧化酶、葡萄糖氧化酶、尿酸氧化酶形成的组中的任意一种。
  9. 根据权利要求7或8所述的离心管，其中，所述待检测物为唾液、血液或尿液。
  10. 根据权利要求7-9中任一项所述的离心管，其中，所述待分析物包括选自由葡萄糖、乳酸盐和尿素形成的组中的任意一种。
  11. 根据权利要求2-10中任一项所述的离心管，其中，相对于所述管体的底部，所述电化学传感器离所述管体的开口更近。
  12. 根据权利要求2-11中任一项所述的离心管，还包括引线，
    其中，所述引线的一端与所述至少一个参考电极、所述至少一个工作电极和所述至少一个对电极中的任一个电极电连接，并且所述引线的另一端与所述至少一个第一引出电极、所述至少一个第二引出电极和所述至少一个第三引出电极中的对应电极电连接，以及
    所述引线包括：沿所述管体的所述内表面延伸的第一部分和沿所述管体的所述外表面延伸的第二部分。
  13. 一种检测系统，包括检测装置以及权利要求1-12中任一项所述的离心管，所述检测装置配置为与所述离心管信号连接，以处理来自所述传感器的电信号。
  14. 根据权利要求13所述的检测系统，还包括离心机和与所述离心机转动连接的离心盘，
    其中，所述离心盘配置为固定所述离心管，以及
    所述离心机包括侧壁，所述检测装置在所述侧壁上。
  15. 根据权利要求14所述的检测系统，其中，所述检测装置包括检测电路、至少一个第一检测电极、至少一个第二检测电极以及至少一个第三检测电极，所述至少一个第一检测电极配置为与所述至少一个第一引出电极一一对应地电连接，所述至少一个第二检测电极配置为与所述至少一个第二引出电极一一对应地电连接，所述至少一个第三检测电极配置为与所述第三引出电极一一对应地电连接，并且所述第一检测电极、所述第二检测电极和所述第三检测电极与所述检测电路信号连接。
  16. 根据权利要求15所述的检测系统，其中，所述至少一个第一检测电极的位置与所述至少一个第一引出电极的位置一一对应，所述至少一个第二检测电极的位置与所述至少一个第二引出电极的位置一一对应，所述至少一个第三检测电极的位置与所述至少一个第三引出电极的位置对应。
  17. 根据权利要求15或16所述的检测系统，还包括：至少一个第一弹性件、至少一个第二弹性件和至少一个第三弹性件，
    其中，所述至少一个第一检测电极一一对应地通过所述至少一个第一弹性件与所述离心机的所述侧壁连接，所述至少一个第二检测电极一一对应地通过所述至少一个第二弹性件与所述离心机的所述侧壁连接，所述至少一个第三检测电极一一对应地通过所述第三弹性件与所述离心机的所述侧壁连接。
  18. 根据权利要求17所述的检测系统，还包括电磁铁，所述电磁铁与所述离心机连接，并且所述电磁铁配置为在通电的情况下朝向所述侧壁吸引所述至少一个第一检测电极、所述至少一个第二检测电极以及所述至少一个第三检测电极。
  19. 一种用于检测系统的检测方法，
    所述检测系统包括：检测装置以及离心管，
    所述离心管包括：
    管体，包括内表面和外表面；
    传感器，在所述管体的所述内表面，并包括第一电极组；
    第二电极组，在所述管体的所述外表面上并与所述第一电极组电连 接，
    所述检测装置配置为与所述离心管信号连接，以处理来自所述传感器的电信号，
    所述检测方法包括：
    将待检测物放入所述离心管，进行离心处理，获得上层清液和沉淀物并使得所述传感器的所述第一电极组与所述离心管内的所述上层清液接触；
    将所述第二电极组与所述检测装置电连接；以及
    通过所述检测装置对所述传感器的电信号进行处理，获取所述待检测物中待分析物的目标参数的值。
  20. 根据权利要求19所述的检测方法，其中，
    所述第二电极组包括至少一个第一引出电极、至少一个第二引出电极和至少一个第三引出电极；以及
    所述将所述第二电极组与所述检测装置电连接，包括：
    将所述检测装置顶住所述离心管的所述外表面；
    检测出所述检测装置没有接收到所述传感器的电信号；
    转动所述离心管，使得所述检测装置与所述至少一个第一引出电极、所述至少一个第二引出电极和所述至少一个第三引出电极信号连接。
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