WO2020218877A1

WO2020218877A1 - Diagnostic device and method for operating diagnostic device

Info

Publication number: WO2020218877A1
Application number: PCT/KR2020/005442
Authority: WO
Inventors: 김희준; 송규정; 육종석; 김성준
Original assignee: 프리시젼바이오 주식회사
Priority date: 2019-04-25
Filing date: 2020-04-24
Publication date: 2020-10-29

Abstract

The present invention provides a method for operating a diagnostic device, the method comprising the steps of: putting a sample including a reactant into a reaction well; pouring a buffer solution into the reaction well; reacting the reactant with conjugates in the reaction well to form a reaction product; removing the buffer solution from the reaction well; and measuring the reaction product.

Description

Diagnostic device driving method and diagnostic device

The present invention relates to a method of driving a diagnostic device and a diagnostic device. More specifically, the present invention relates to a driving method and a diagnostic device for performing diagnosis by reacting a conjugate and a reactant.

In keeping with the recent era of prevention and personalized medicine, the in vitro diagnostic industry is in the spotlight. The in vitro diagnostic industry is a field in which various diseases such as diabetes, cholesterol, cancer, etc. can be checked with a drop of blood, urine, etc., and in vitro diagnostic devices that enable users to check various diseases at home or while carrying are being developed. .

The core competitiveness in the development of in vitro diagnostic devices is the fusion of biotechnology that develops biomaterials (antigens, antibodies, genes, enzymes, etc.) that react with target substances and information technology that develops devices to measure them. Most of the world's in vitro diagnostic companies are leading high value-added knowledge-based industries, generating enormous sales and net profits through such technology convergence.

An object of the present invention is to provide a method of driving a diagnostic device and a diagnostic device capable of inducing an effective reaction between a conjugate and a reactant.

The present invention includes the steps of introducing a sample containing a reactant into a reaction well; Injecting a buffer solution into the reaction well; Forming a reaction product by reacting the conjugates in the reaction well and the reactant; Removing the buffer solution in the reaction well; And it provides a method of driving a diagnostic device comprising the step of measuring the reaction result.

Forming the reaction product may include rotating the second magnet in the reaction well.

Rotating the second magnet may include rotating the first magnet outside the reaction well to rotate the second magnet.

The rotation of the second magnet may include rotation of the second magnet so that the third magnet in the reaction well separates from the second magnet.

The step of forming the reaction product may include reacting the first conjugate connected to the third magnet and the second conjugate connected to the light-emitting body with the reactant.

The step of removing the buffer solution in the reaction well may include attaching the third magnet to the second magnet in the reaction well, and not moving the reaction product together with the buffer solution.

The step of removing the buffer solution in the reaction well may include removing the light-emitting body and the second assembly that are not connected to the third magnet together with the buffer solution.

The step of measuring the reaction result may include uniformly distributing the light emitter by stirring the buffer solution in the reaction well.

The present invention is a reaction well comprising an inner space; A first magnet other than the reaction well; A second magnet provided in the reaction well and spaced apart from the first magnet by the reaction well; A third magnet in the reaction well; A first bonding body connected to the third magnet; A light emitter in the reaction well; And a second conjugate connected to the light-emitting body.

The first magnet may be rotatable.

The second magnet may rotate according to the rotation of the first magnet.

The first conjugate may be bonded to the reactant, and the second conjugate may be bonded to the reactant.

The maximum length of the first magnet may be longer than the maximum length of the second magnet.

It may further include a measuring unit for measuring the light emitted in the reaction well.

The reaction well, a base; Sidewalls protruding from the edge of the upper surface of the base; And

It may include a second opening penetrating through the sidewall.

The functional group of the third magnet and the functional group of the first conjugate are bonded to each other to connect the third magnet and the first conjugate, and the functional group of the luminous material and the functional group of the second conjugate are bonded to each other to the light-emitting body and the second conjugate. Can be connected.

A method of driving a diagnostic device and a diagnostic device according to the present invention include stirring a buffer solution in the reaction well by rotating a magnet in the reaction well, so that the conjugate and the reactant in the reaction well can react effectively.

1 is a cross-sectional view of a diagnostic device according to an embodiment of the present invention.

2 is a flowchart illustrating a method of driving a diagnostic device according to an embodiment of the present invention.

3A, 3B, 3C, 3D, and 3E are diagrams for explaining a method of driving a diagnostic apparatus according to an exemplary embodiment of the present invention.

Advantages and features of the present invention, and a method of achieving them will become apparent with reference to the embodiments described below in detail together with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms. It is provided to completely inform the scope of the invention to the possessor, and the invention is only defined by the scope of the claims. The same reference numerals refer to the same elements throughout the specification.

The terms used in the present specification are for describing exemplary embodiments and are not intended to limit the present invention. In this specification, the singular form also includes the plural form unless specifically stated in the phrase. As used in the specification,'comprises' and/or'comprising' refers to the presence of one or more other components, steps, actions and/or devices, and/or the recited component, step, action and/or device. Or does not exclude additions. Hereinafter, embodiments of the present invention will be described in detail.

1 is a cross-sectional view of a reaction diagnosis apparatus according to an embodiment of the present invention.

Referring to Figure 1, the reaction induction device according to an embodiment of the present invention is a reaction well (reaction well, 110), a first magnet 120, a second magnet 130, third magnets 140, the first It may include conjugates 150, light emitters 160, second conjugates 170, and measurement unit 200.

The reaction well 110 may have a cylindrical shape with an empty inside. The reaction well 110 may include a base 111, a sidewall 112 and an inner space 113. The base 111 may have a planar circular plate shape. The sidewall 112 may protrude upward from the edge of the upper surface of the base 111. The inner space 113 may be defined by the base 111 and the sidewall 112. The sidewall 112 may planarly surround the inner space 113.

The reaction well 110 may further include a first opening 114 and a second opening 115. The inner space 113 of the reaction well 110 may communicate with a space outside the reaction well 110 by the first and

second openings

114 and 115. The first opening 114 may communicate the external space above the reaction well 110 and the internal space 113 of the reaction well 110. The first opening 114 may be surrounded in a plane by an upper portion of the sidewall 112. The second opening 115 may penetrate through the sidewall 112 of the reaction well 110. The second opening 115 may communicate the external space on the side of the reaction well 110 and the internal space 113 of the reaction well 110.

The first magnet 120 may be disposed outside the reaction well 110. The first magnet 120 may be disposed under the base 111 of the reaction well 110. For example, the first magnet 120 may have a bar shape. The maximum length of the first magnet 120 may be smaller than the diameter of the base 111 of the reaction well 110.

In the inner space 113 of the reaction well 110, the second magnet 130, the third magnets 140, the first bonding bodies 150, the luminous bodies 160, and the second bonding bodies 170 are Can be provided. The second magnet 130 may be disposed on the base 111 of the reaction well 110. The second magnet 130 may be spaced apart from the first magnet 120 with the base 111 of the reaction well 110 interposed therebetween. The second magnet 130 may have a bar shape. The maximum length of the second magnet 130 may be smaller than the maximum length of the first magnet 120.

The second and

third magnets

130 and 140 may attract each other by magnetic force acting between the second and

third magnets

130 and 140. Each of the third magnets 140, the first conjugates 150, the light emitters 160, and the second conjugates 170 may include a functional group. The third magnets 140 and the light emitters 160 may include a functional group by surface treatment. The functional group of the first assembly 150 and the functional group of the third magnet 140 are bonded to each other, so that the first assembly 150 may be connected to the third magnet 140. The functional group of the second conjugate 170 and the functional group of the light-emitting body 160 are bonded to each other, so that the second conjugate 170 may be connected to the light-emitting body 160.

The first magnet 120 may be rotatable under the base 111 of the reaction well 110. As the first magnet 120 rotates, the second magnet 130 may rotate.

The measurement unit 200 may be disposed on the reaction well 110. The measurement unit 200 may measure light emitted from the reaction well 110.

For example, the measurement unit 200 may include a light source, an optical sensor, and a processor. In this case, the measurement unit 200 irradiates light into the reaction well 110 from a light source, obtains the light emitted from the reaction well 110 by the irradiated light by an optical sensor, and then obtained using a processor. Light can be analyzed.

As another example, the measurement unit 200 may include an optical sensor and a processor. In this case, the measurement unit 200 may acquire light emitted from the reaction well 110 by an optical sensor and then analyze the acquired light using a processor.

Referring to FIG. 2, in the method of driving a diagnostic device according to an embodiment of the present invention, in the step of injecting a sample into a reaction well (S100), injecting a buffer solution into the reaction well (S200), and forming a reaction result. (S300), a step of removing the buffer solution from the reaction well (S400), a washing step (S500), and a measurement step (S600) may be included.

Referring to FIGS. 2 and 3A, in step S100 of introducing a sample into the reaction well, a sample SA may be injected into the reaction well 110. The sample SA may be introduced into the inner space 113 of the reaction well 110 through the first opening 114 of the reaction well 110. For example, the sample SA may be blood. The sample SA may include reactants RM. The reactant RM may be a material that reacts with the first conjugate 150 and the second conjugate 170 in the reaction well 110 and binds to the first conjugate 150 and the second conjugate 170. . For example, the first conjugate 150 and the second conjugate 170 and the reactant RM may react with an antigen antibody. For another example, the first conjugate 150 and the second conjugate 170 and the reactant RM may react with Streptavidin-Biotin.

Referring to FIGS. 2 and 3B, in the step S200 of introducing the buffer solution into the reaction well, the buffer solution BL may be injected into the reaction well 110. The buffer solution BL may be injected into the inner space 113 of the reaction well 110 through the second opening 115 of the reaction well 110. The second opening 115 of the reaction well 110 may be open or closed. By closing the second opening 115 of the reaction well 110, the buffer solution BL filling the inner space 113 of the reaction well 110 may not escape to the outside of the reaction well 110.

The sample SA may be diluted by the buffer solution BL injected into the reaction well 110. When the sample SA is diluted in the buffer solution BL, the reactants RM of the sample SA may maintain their shape and properties without reacting with the buffer solution BL.

2 and 3C, in the step of forming a reaction product (S300), the first and

second conjugates

150 and 170 and the reactant RM are reacted to form a reaction product RR. have. The reaction result RR may include a third magnet 140, a first conjugate 150, a light emitter 160, a second conjugate 170, and a reactant RM.

The step of forming the reaction product (S300) may include raising the temperature in the reaction well 110 and stirring the buffer solution BL in the reaction well 110.

As an example, a heat source may be disposed around the reaction well 110 to increase the temperature in the reaction well 110. The temperature in the reaction well 110 may rise to a temperature at which the first and

second conjugates

150 and 170 and the reactant RM can react.

Stirring the buffer solution BL in the reaction well 110 may include rotating the first magnet 120. The first magnet 120 may rotate by a separate external force. For example, the first magnet 120 may be rotated by connecting a rotation motor to the first magnet 120. As the first magnet 120 rotates, the second magnet 130 may rotate. The second magnet 130 may rotate according to a change in the magnetic field around the first magnet 120 due to the rotation of the first magnet 120. When the second magnet 130 rotates, the third magnets 140 may fall from the second magnet 130 by centrifugal force. By the rotation of the second magnet 130, the buffer solution BL in the reaction well 110 may be stirred.

As the temperature in the reaction well 110 rises and the buffer solution BL in the reaction well 110 is stirred, the first and

second conjugates

150 and 170 and the reactant RM in the reaction well 110 ) Can react. In other words, the first and

second conjugates

150 and 170 may be connected to the reactant RM. According to the reaction between the first and

second conjugates

150 and 170 and the reactant RM, the third magnet 140, the first and

second conjugates

150 and 170, the reactant RM, and the light emitter A reaction product RR including 160 may be formed.

2 and 3D, in the step of removing the buffer solution from the reaction well (S400), the second opening 115 of the reaction well 110 is opened to allow the buffer solution BL in the reaction well 110 to be removed. It can be moved to the outside of the reaction well 110.

When the rotation of the second magnet 130 is stopped, the third magnets 140 of the reaction products RR may be attached to the second magnet 130. When the buffer solution BL moves outside the reaction well 110, as the third magnets 140 are attached to the second magnet 130, the reaction products RR react together with the buffer solution BL. It may not move outside the well 110. In other words, the reaction products RR may remain in the reaction well 110. Materials other than the reaction products RR (for example, the light-emitting body 160 and the second conjugate 170 that are not connected to the third magnet 140) are formed with the buffer solution BL and the reaction well 110 ) Can be moved outside. In other words, the remaining materials may be removed from the reaction well 110.

Subsequently, the cleaning step S500 may be performed. The cleaning step (S500) is to fill the buffer solution BL in the reaction well 110 similar to that described in FIG. 3B, and agitate the buffer solution BL in the reaction well 110 similar to that described in FIG. 3C. It may include removing the buffer solution BL in the reaction well 110, similar to that described with reference to FIG. 3D.

By the cleaning step (S500), the remaining materials (for example, the luminous body 160 and the second assembly 170 that are not connected to the third magnet 140) other than the reaction product RR are removed from the reaction well ( 110) can be removed. Accordingly, measurement of the reaction products RR can be made relatively accurately.

2 and 3E, in the measurement step S600, the reaction result RR may be measured. Measuring the reaction result RR may include measuring the light by the luminous body 160 in the reaction well 110. For example, the light-emitting body 160 may be a phosphor. As another example, the light emitter 160 may be a self light emitter.

Similar to the one described in FIG. 3B, the buffer solution BL may be filled in the reaction well 110, and the buffer solution BL in the reaction well 110 may be stirred similarly to the one described in FIG. 3C. After stirring the buffer solution BL to uniformly distribute the light-emitting body 160 of the reaction product RR in the buffer solution BL, light may be obtained using the measurement unit 200. For example, the measurement unit 200 may irradiate the incident light L1 into the reaction well 110, and the emission light L2 emitted from the reaction well 110 may be obtained from the measurement unit 200.

By analyzing the acquired light, it can be diagnosed whether the sample SA has a specific reactant RM. By removing the buffer solution from the reaction well (S400) and cleaning (S500), the luminous body 160 must be connected to the third magnet 140 to remain in the reaction well 110, so that the sample SA The luminous body 160 can remain in the reaction well 110 only when the luminous body 160 has a reactant RM that reacts with the first and

second conjugates

150 and 170, and the light emitted from the luminous body 160 is measured. Can be obtained with (200).

In the above, embodiments of the present invention have been described with reference to the accompanying drawings, but those of ordinary skill in the art to which the present invention pertains can be implemented in other specific forms without changing the technical spirit or essential features. You can understand that there is. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting.

Claims

Introducing a sample including a reactant into a reaction well;

Injecting a buffer solution into the reaction well;

Forming a reaction product by reacting the conjugates in the reaction well and the reactant;

Removing the buffer solution in the reaction well; And

A method of driving a diagnostic device comprising the step of measuring the reaction result.
The method of claim 1,

The step of forming the reaction product,

A method of driving a diagnostic device comprising rotating a second magnet in the reaction well.
The method of claim 2,

The rotation of the second magnet,

A driving method of a diagnostic device comprising rotating the second magnet by rotating the first magnet outside the reaction well.
The method of claim 2,

The rotation of the second magnet,

And a third magnet in the reaction well separated from the second magnet by rotating the second magnet.
The method of claim 1,

The step of forming the reaction product,

A driving method of a diagnostic device, comprising reacting a first conjugate connected to a third magnet and a second conjugate connected to a light emitter with the reactant.
The method of claim 5,

The step of removing the buffer solution in the reaction well,

Attaching the third magnet to the second magnet in the reaction well, and not moving the reaction product together with the buffer solution.
The method of claim 6,

The step of removing the buffer solution in the reaction well,

And removing the light-emitting body and the second assembly that are not connected to the third magnet together with the buffer solution.
The method of claim 1,

The step of measuring the reaction product,

A method of driving a diagnostic device comprising uniformly distributing a light emitter by stirring the buffer solution in the reaction well.
A reaction well including an internal space;

A first magnet other than the reaction well;

A second magnet provided in the reaction well and spaced apart from the first magnet by the reaction well;

A third magnet in the reaction well;

A first bonding body connected to the third magnet;

A light emitter in the reaction well; And

A diagnostic device comprising a second conjugate connected to the luminous body.
The method of claim 9,

The first magnet is rotatable diagnostic device.
The method of claim 10,

The second magnet is a diagnostic device that rotates according to the rotation of the first magnet.
The method of claim 9,

The first conjugate can be combined with a reactant,

The second conjugate is a diagnostic device capable of being combined with the reactant.
The method of claim 9,

The diagnostic device in which the maximum length of the first magnet is longer than the maximum length of the second magnet.
The method of claim 9,

Diagnosis device further comprising a measurement unit for measuring the light emitted in the reaction well.
The method of claim 9,

The reaction well,

Base;

Sidewalls protruding from the edge of the upper surface of the base; And

A diagnostic device comprising a second opening penetrating the sidewall.
The method of claim 9,

A functional group of the third magnet and a functional group of the first bonding body are bonded to each other to connect the third magnet and the first bonded body,

A diagnostic device in which the functional group of the luminous body and the functional group of the second conjugate are joined to connect the luminous body and the second conjugate.