WO2020211504A1 - Polypeptide hydrogel and preparation method therefor - Google Patents

Polypeptide hydrogel and preparation method therefor Download PDF

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Publication number
WO2020211504A1
WO2020211504A1 PCT/CN2020/073061 CN2020073061W WO2020211504A1 WO 2020211504 A1 WO2020211504 A1 WO 2020211504A1 CN 2020073061 W CN2020073061 W CN 2020073061W WO 2020211504 A1 WO2020211504 A1 WO 2020211504A1
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polypeptide
hydrogel
preparation
self
peptide
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PCT/CN2020/073061
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French (fr)
Chinese (zh)
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汪少芸
陈惠敏
何庆燕
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福州大学
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00—Processes of treating or compounding macromolecular substances
    • C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
    • C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
    • C08J3/075—Macromolecular gels
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2389/00—Characterised by the use of proteins; Derivatives thereof

Definitions

  • the invention belongs to the technical field of biological materials, and specifically relates to a polypeptide hydrogel and a preparation method thereof.
  • Self-assembled peptide hydrogels are widely used in tissue engineering, drug delivery, cell culture and other fields due to their good biocompatibility, reversibility and degradability.
  • the types of peptides that can self-assemble into hydrogels include amino acid paired peptides, ⁇ -hairpin peptides, Fluorenylmethoxycarbonyl (Fmoc) peptides and amphiphilic peptides, but these peptides that can self-assemble into hydrogels have longer sequences. Often contain synthetic non-degradable end groups. The self-assembly of natural oligopeptides (4-8 amino acids) has received less research and attention.
  • Engineering peptide-based hydrogels introduce functional molecules into some natural peptide fragments to make the hydrogel have certain specific functions, but most of the engineering peptide-based hydrogels are now designed based on the original self-assembled peptide system The design is limited by the above modification, so it is necessary and meaningful to develop a new self-assembled peptide system.
  • the purpose of the present invention is to overcome the existing research ideas and methods of polypeptide hydrogels at home and abroad in view of the shortcomings of the prior art, and provide a polypeptide hydrogel and a preparation method thereof.
  • the polypeptide sequence of the polypeptide hydrogel is short in length, the gel forming method is simple and rapid, and the obtained polypeptide hydrogel has good biocompatibility.
  • a self-assembled polypeptide to form a hydrogel its amino acid sequence is: Glu-Ile-Trp-Leu-Lys.
  • a polypeptide hydrogel is a hydrogel formed by adjusting the pH and concentration of a polypeptide solution formed by self-assembled polypeptides to form a hydrogel.
  • a method for preparing a polypeptide hydrogel the specific steps are: the polypeptides that self-assemble to form a gel are prepared into a polypeptide solution of a certain concentration in a PBS buffer of a certain pH, vortexed until the polypeptide powder is dissolved, and allowed to stand at room temperature for condensation Gelatinize.
  • the pH range of the PBS buffer in the above preparation method is: pH 5-8.
  • the pH of the PBS buffer in the above preparation method is 7.
  • the concentration range of the polypeptide solution in the above preparation method is: 7.27-14.54 mM.
  • the standing time in the above preparation method is: 30 min.
  • the significant advantages of the present invention are: a polypeptide hydrogel provided by the present invention and a preparation method thereof, the polypeptide sequence of the polypeptide self-assembled to form the hydrogel is short; the preparation method of the polypeptide hydrogel is simple and rapid; the obtained polypeptide
  • the hydrogel has good biocompatibility and reversibility; it provides flexibility for the chemical design of preparing peptide-based hydrogels, provides simplicity for peptide synthesis, and facilitates the development and utilization of peptide-based hydrogel materials.
  • Figure 1 shows the gelation phase diagram under different peptide concentrations and different pH conditions.
  • Sol peptide solution
  • Gel peptide hydrogel
  • Precipate peptide precipitation.
  • Figure 2 is a TEM image of a 14.54 mM, pH 7.0 peptide hydrogel, and the inset in the upper right corner is a macroscopic gelation image.
  • Figure 3 shows the storage modulus of 14.54 mM polypeptide hydrogel under different pH conditions.
  • Figure 4 is a graph showing the influence of 14.54 mM, pH 7.0 polypeptide hydrogel on HepG2 cell activity.
  • Control control group
  • EK experimental group.
  • a self-assembled polypeptide to form a hydrogel its amino acid sequence is: Glu-Ile-Trp-Leu-Lys.
  • the method for obtaining the polypeptide that self-assembles to form a hydrogel is: design a self-assembled polypeptide through theory, and use solid-phase synthesis technology for polypeptide synthesis.
  • a method for preparing polypeptide hydrogels the specific steps are: PBS buffers of different pH (pH3, pH4, pH5, pH6, pH7, pH8, pH9) are configured to different concentrations (7.27, 8.72, 10.18, 11.63, 13.08, 14.54mM) self-assembled peptide solution to form a hydrogel, vortex until the peptide powder is dissolved, and let it stand overnight at room temperature. Use an inverted test tube to determine whether the solution forms a peptide hydrogel. If the sample does not slip off in an inverted test tube, that is It is a peptide hydrogel.
  • the gelation phase diagram of the polypeptide hydrogel prepared in this example under different polypeptide concentrations and different pH conditions is shown in Figure 1.
  • the results from Fig. 1 show that the gel forming conditions of the polypeptide are in a "v" shape, that is, when the polypeptide concentration is low, the gelation pH range is narrow, and as the concentration increases, the gelation pH range increases. Therefore, in a wide range of pH 5-8, peptides can self-assemble to form hydrogels.
  • a polypeptide hydrogel the preparation method is as follows: Weigh the polypeptide powder assembled to form the hydrogel into a 2mL tube, add pH 7.0 PBS buffer to prepare a polypeptide solution with a concentration of 14.54 mM, and vortex to the powder Fully dissolve, stand at room temperature for 30 minutes to prepare polypeptide hydrogel. Take 10 ⁇ L of gel and drop it on the copper net. After 8 minutes, absorb the excess polypeptide hydrogel with filter paper. Next, take 10 ⁇ L of sodium phosphotungstate solution for negative staining. After 8 minutes, absorb the excess sodium phosphotungstate solution with filter paper and dry at room temperature. Overnight, observe its microstructure on a transmission electron microscope machine.
  • Figure 2 shows the TEM and macroscopic gel formation diagram of the 14.54 mM, pH 7.0 polypeptide hydrogel prepared in this example.
  • Figure 2 can visually see that the peptides self-assemble to form a solid-like hydrogel with a three-dimensional network structure, and the presence of nanofibers can be observed from the TEM image. This is because the pentapeptide has N'and C'at pH 7
  • the terminal amino groups and carboxyl groups are protonated, each with positive and negative charges, and self-assemble through electrostatic interaction to form nanofibers.
  • the fibers are further entangled to "lock" water molecules, and finally form a hydrogel with a three-dimensional network structure.
  • a polypeptide hydrogel the preparation method is as follows: Weigh the polypeptide powder self-assembled to form the hydrogel into a 2mL tube, add PBS buffers of different pH (pH5, pH6, pH7, pH8) to the concentration of 14.54 mM polypeptide solution, vortex until the powder is fully dissolved, and let it stand at room temperature for 30 minutes to form a polypeptide hydrogel.
  • PBS buffers of different pH (pH5, pH6, pH7, pH8)
  • Figure 3 shows the storage modulus diagram of the 14.54 mM polypeptide hydrogel in this example under different pH conditions.
  • Figure 3 The results show that when the pH is 7, its storage modulus reaches the maximum and has the best rheological properties.
  • a polypeptide hydrogel is prepared by weighing the polypeptide powder that has been assembled to form a hydrogel into a 2 mL tube, adding pH 7 PBS buffer to prepare a polypeptide solution with a concentration of 14.54 mM, and vortexing until the powder is fully dissolved Put 2 ⁇ L of the peptide hydrogel in a 96-well plate with HepG2 cells for culture. After 24 hours, add 20 ⁇ L MTT (5mg/mL) and continue to incubate for 4 hours. After aspirating the supernatant, add 150 ⁇ L of DMSO to each well, mix with a microplate shaker, and measure the absorbance at 570 nm.
  • the control group is a blank control that replaces 2 ⁇ L of peptide hydrogel with 2 ⁇ L of sterile water. Contains peptide hydrogel.
  • Figure 4 shows that the polypeptide hydrogel has no toxic side effects on HepG2 cells, and its activity can reach 100%, indicating that the polypeptide hydrogel has good biocompatibility.

Abstract

The present invention belongs to the technical field of biological materials, and provides a polypeptide capable of self-assembling and forming a hydrogel, a hydrogel thereof and a preparation method therefor. The amino acid sequence of the polypeptide is Glu-Ile-Trp-Leu-Lys. The sequence length of the polypeptide is short, the prepared polypeptide hydrogel has good biocompatibility, and the gel forming process is simple and rapid.

Description

一种多肽水凝胶及其制备方法Polypeptide hydrogel and preparation method thereof 技术领域Technical field
本发明属于生物材料技术领域，具体涉及一种多肽水凝胶及其制备方法。The invention belongs to the technical field of biological materials, and specifically relates to a polypeptide hydrogel and a preparation method thereof.
背景技术Background technique
自组装多肽水凝胶由于其良好的生物相容性、可逆性和可降解性等特点，被广泛应用于组织工程、药物递送和细胞培养等领域。可自组装成水凝胶的多肽类型有氨基酸配对型多肽、β-发夹型多肽、Fluorenylmethoxycarbonyl (Fmoc)肽和两亲性多肽，但这些能够自组装成水凝胶的肽段序列较长，往往含有合成的不可降解末端基。天然的寡肽(4-8个氨基酸)自组装受到较少研究和关注。Self-assembled peptide hydrogels are widely used in tissue engineering, drug delivery, cell culture and other fields due to their good biocompatibility, reversibility and degradability. The types of peptides that can self-assemble into hydrogels include amino acid paired peptides, β-hairpin peptides, Fluorenylmethoxycarbonyl (Fmoc) peptides and amphiphilic peptides, but these peptides that can self-assemble into hydrogels have longer sequences. Often contain synthetic non-degradable end groups. The self-assembly of natural oligopeptides (4-8 amino acids) has received less research and attention.
技术问题technical problem
工程肽基水凝胶是在一些天然多肽的片段上引入功能分子，使其水凝胶具有某些特定功能，但是现在大多数的工程肽基水凝胶的设计是在原有的自组装多肽体系上进行改造，其设计受到限制，因此开发新的自组装多肽体系是十分必要且有意义的。Engineering peptide-based hydrogels introduce functional molecules into some natural peptide fragments to make the hydrogel have certain specific functions, but most of the engineering peptide-based hydrogels are now designed based on the original self-assembled peptide system The design is limited by the above modification, so it is necessary and meaningful to develop a new self-assembled peptide system.
技术解决方案Technical solutions
本发明的目的在于针对现有技术的不足，突破了国内外现存的多肽水凝胶的研究思路和方法，提供一种多肽水凝胶及其制备方法。该多肽水凝胶的多肽序列长度短，成胶方式简便、快速，得到的多肽水凝胶生物相容性好。The purpose of the present invention is to overcome the existing research ideas and methods of polypeptide hydrogels at home and abroad in view of the shortcomings of the prior art, and provide a polypeptide hydrogel and a preparation method thereof. The polypeptide sequence of the polypeptide hydrogel is short in length, the gel forming method is simple and rapid, and the obtained polypeptide hydrogel has good biocompatibility.
为实现上述目的，采用以下技术方案：To achieve the above objectives, the following technical solutions are adopted:
一种自组装形成水凝胶的多肽，其氨基酸序列为：Glu-Ile-Trp-Leu-Lys。A self-assembled polypeptide to form a hydrogel, its amino acid sequence is: Glu-Ile-Trp-Leu-Lys.
上述自组装形成水凝胶的多肽在制备水凝胶中的应用。Application of the above self-assembled polypeptide to form hydrogel in preparing hydrogel.
一种多肽水凝胶，通过调节自组装形成水凝胶的多肽形成的多肽溶液的pH及浓度来进行凝胶化而形成的水凝胶。A polypeptide hydrogel is a hydrogel formed by adjusting the pH and concentration of a polypeptide solution formed by self-assembled polypeptides to form a hydrogel.
一种多肽水凝胶的制备方法，具体步骤为：将自组装形成凝胶的多肽以一定pH的PBS缓冲液，配置成一定浓度的多肽溶液，涡旋至多肽粉末溶解，于室温静置凝胶化。A method for preparing a polypeptide hydrogel, the specific steps are: the polypeptides that self-assemble to form a gel are prepared into a polypeptide solution of a certain concentration in a PBS buffer of a certain pH, vortexed until the polypeptide powder is dissolved, and allowed to stand at room temperature for condensation Gelatinize.
上述制备方法中PBS缓冲液的pH范围为：pH 5~8。The pH range of the PBS buffer in the above preparation method is: pH 5-8.
优选的，上述制备方法中PBS缓冲液的pH为7。Preferably, the pH of the PBS buffer in the above preparation method is 7.
上述制备方法的中多肽溶液的浓度范围为：7.27~14.54 mM。The concentration range of the polypeptide solution in the above preparation method is: 7.27-14.54 mM.
上述制备方法的中静置时间为：30min。The standing time in the above preparation method is: 30 min.
有益效果Beneficial effect
本发明的显著优势在于：本发明提供的一种多肽水凝胶及其制备方法，其自组装形成水凝胶的多肽的多肽序列长度短；多肽水凝胶的制备方法简便、快速；所得多肽水凝胶的生物相容性好且可逆；为制备肽基水凝胶的化学设计提供灵活性，为多肽合成提供简便性，有助于肽基水凝胶材料的开发利用。The significant advantages of the present invention are: a polypeptide hydrogel provided by the present invention and a preparation method thereof, the polypeptide sequence of the polypeptide self-assembled to form the hydrogel is short; the preparation method of the polypeptide hydrogel is simple and rapid; the obtained polypeptide The hydrogel has good biocompatibility and reversibility; it provides flexibility for the chemical design of preparing peptide-based hydrogels, provides simplicity for peptide synthesis, and facilitates the development and utilization of peptide-based hydrogel materials.
附图说明Description of the drawings
图1为不同多肽浓度、不同pH条件下的成胶相图。Sol：多肽溶液；Gel：多肽水凝胶；Precipate：多肽沉淀。Figure 1 shows the gelation phase diagram under different peptide concentrations and different pH conditions. Sol: peptide solution; Gel: peptide hydrogel; Precipate: peptide precipitation.
图2为14.54 mM、pH7.0的多肽水凝胶的TEM图，右上角插图为宏观成胶图。Figure 2 is a TEM image of a 14.54 mM, pH 7.0 peptide hydrogel, and the inset in the upper right corner is a macroscopic gelation image.
图3为14.54 mM的多肽水凝胶在不同pH条件下的储能模量图。Figure 3 shows the storage modulus of 14.54 mM polypeptide hydrogel under different pH conditions.
图4为14.54 mM、pH7.0的多肽水凝胶对HepG2细胞活性的影响图。Control：对照组；EK：实验组。Figure 4 is a graph showing the influence of 14.54 mM, pH 7.0 polypeptide hydrogel on HepG2 cell activity. Control: control group; EK: experimental group.
本发明的实施方式Embodiments of the invention
以下通过实施例对本发明做进一步的说明。The following examples further illustrate the present invention.
实施例Example 11
一种自组装形成水凝胶的多肽，其氨基酸序列为：Glu-Ile-Trp-Leu-Lys。A self-assembled polypeptide to form a hydrogel, its amino acid sequence is: Glu-Ile-Trp-Leu-Lys.
 该自组装形成水凝胶的多肽的获得方法为：通过理论设计可自组装的多肽，并利用固相合成技术进行多肽合成。The method for obtaining the polypeptide that self-assembles to form a hydrogel is: design a self-assembled polypeptide through theory, and use solid-phase synthesis technology for polypeptide synthesis.
实施例Example 22
一种多肽水凝胶的制备方法，具体步骤为：以不同pH（pH3、pH4、pH5、pH6、pH7、pH8、pH9）的PBS缓冲液，配置成不同浓度（7.27、8.72、10.18、11.63、13.08、14.54mM）的自组装形成水凝胶的多肽溶液，涡旋至多肽粉末溶解，于室温静置过夜，通过倒立试管判断溶液是否形成多肽水凝胶，若倒立试管样品不会滑落，即为多肽水凝胶。A method for preparing polypeptide hydrogels, the specific steps are: PBS buffers of different pH (pH3, pH4, pH5, pH6, pH7, pH8, pH9) are configured to different concentrations (7.27, 8.72, 10.18, 11.63, 13.08, 14.54mM) self-assembled peptide solution to form a hydrogel, vortex until the peptide powder is dissolved, and let it stand overnight at room temperature. Use an inverted test tube to determine whether the solution forms a peptide hydrogel. If the sample does not slip off in an inverted test tube, that is It is a peptide hydrogel.
本实施例制备获得的多肽水凝胶，其在不同多肽浓度、不同pH条件下的成胶相图见图1。从图1结果表明，多肽的成胶条件呈现“v”型，即多肽浓度较低时，成胶的pH范围窄，随着浓度的增加，成胶的pH范围增大。由此得，在pH5~8的较宽范围下，多肽能自组装形成水凝胶。The gelation phase diagram of the polypeptide hydrogel prepared in this example under different polypeptide concentrations and different pH conditions is shown in Figure 1. The results from Fig. 1 show that the gel forming conditions of the polypeptide are in a "v" shape, that is, when the polypeptide concentration is low, the gelation pH range is narrow, and as the concentration increases, the gelation pH range increases. Therefore, in a wide range of pH 5-8, peptides can self-assemble to form hydrogels.
实施例Example 33
一种多肽水凝胶，其制备方法为： 称取自组装形成水凝胶的多肽粉末于2mL管中，加入pH7.0的PBS缓冲液，配置成浓度为14.54mM的多肽溶液，旋涡至粉末充分溶解，室温下静置30min制成多肽水凝胶。取10μL凝胶滴在铜网上，8min后用滤纸吸取多余的多肽水凝胶，接下来取10μL 磷钨酸钠溶液进行负染，8min后用滤纸吸取多余磷钨酸钠溶液，于室温下干燥过夜，于透射电镜机上观察其微观结构图。A polypeptide hydrogel, the preparation method is as follows: Weigh the polypeptide powder assembled to form the hydrogel into a 2mL tube, add pH 7.0 PBS buffer to prepare a polypeptide solution with a concentration of 14.54 mM, and vortex to the powder Fully dissolve, stand at room temperature for 30 minutes to prepare polypeptide hydrogel. Take 10μL of gel and drop it on the copper net. After 8 minutes, absorb the excess polypeptide hydrogel with filter paper. Next, take 10μL of sodium phosphotungstate solution for negative staining. After 8 minutes, absorb the excess sodium phosphotungstate solution with filter paper and dry at room temperature. Overnight, observe its microstructure on a transmission electron microscope machine.
本实施例制备获得的14.54mM、pH7.0的多肽水凝胶的TEM及宏观成胶图见图2。图2能直观看到多肽自组装形成具有三维网络结构的固状样的水凝胶，并且从TEM图观测到纳米纤维的存在，这是因为五肽在pH为7时，N＇和C＇端的氨基及羧基质子化，各自带上正电荷和负电荷，通过静电相互作用进行自组装形成纳米纤维，纤维进一步缠绕，“锁住”水分子，最终形成具有三维网络结构的水凝胶。Figure 2 shows the TEM and macroscopic gel formation diagram of the 14.54 mM, pH 7.0 polypeptide hydrogel prepared in this example. Figure 2 can visually see that the peptides self-assemble to form a solid-like hydrogel with a three-dimensional network structure, and the presence of nanofibers can be observed from the TEM image. This is because the pentapeptide has N'and C'at pH 7 The terminal amino groups and carboxyl groups are protonated, each with positive and negative charges, and self-assemble through electrostatic interaction to form nanofibers. The fibers are further entangled to "lock" water molecules, and finally form a hydrogel with a three-dimensional network structure.
实施例Example 44
一种多肽水凝胶，其制备方法为： 称取自组装形成水凝胶的多肽粉末于2mL管中，加入不同 pH（pH5、pH6、pH7、pH8）的PBS缓冲液，配置成浓度为14.54mM的多肽溶液，旋涡至粉末充分溶解，室温下静置30min制成多肽水凝胶。A polypeptide hydrogel, the preparation method is as follows: Weigh the polypeptide powder self-assembled to form the hydrogel into a 2mL tube, add PBS buffers of different pH (pH5, pH6, pH7, pH8) to the concentration of 14.54 mM polypeptide solution, vortex until the powder is fully dissolved, and let it stand at room temperature for 30 minutes to form a polypeptide hydrogel.
本实施例14.54mM的多肽水凝胶在不同pH条件下的储能模量图见图3。图3 结果表明pH为7时，其储能模量达到最大，具有最好的流变性能。Figure 3 shows the storage modulus diagram of the 14.54 mM polypeptide hydrogel in this example under different pH conditions. Figure 3 The results show that when the pH is 7, its storage modulus reaches the maximum and has the best rheological properties.
实施例Example 55
一种多肽水凝胶，其制备方法为： 称取自组装形成水凝胶的多肽粉末于2mL管中，加入pH7的PBS缓冲液，配置成浓度为14.54mM的多肽溶液，旋涡至粉末充分溶解，室温下静置30min制成多肽水凝胶，取2μL的多肽水凝胶加入培养有HepG2细胞的96孔板中进行培养, 24h后加入20μL MTT（5mg/mL），继续培养4 h。吸去上清后，每孔加入150 μL DMSO，微孔板振荡器混匀，测定570 nm处吸光值，对照组为以2μL的无菌水替换2μL的多肽水凝胶的空白对照，即不含有多肽水凝胶。A polypeptide hydrogel is prepared by weighing the polypeptide powder that has been assembled to form a hydrogel into a 2 mL tube, adding pH 7 PBS buffer to prepare a polypeptide solution with a concentration of 14.54 mM, and vortexing until the powder is fully dissolved Put 2μL of the peptide hydrogel in a 96-well plate with HepG2 cells for culture. After 24 hours, add 20μL MTT (5mg/mL) and continue to incubate for 4 hours. After aspirating the supernatant, add 150 μL of DMSO to each well, mix with a microplate shaker, and measure the absorbance at 570 nm. The control group is a blank control that replaces 2 μL of peptide hydrogel with 2 μL of sterile water. Contains peptide hydrogel.
本实施例制备获得的14.54mM、pH7.0的多肽水凝胶对HepG2细胞活性的影响见图4。图4结果表明多肽水凝胶对HepG2细胞无毒副作用，其活性可达到100%，说明多肽水凝胶具有良好的生物相容性。The effect of the 14.54 mM, pH 7.0 polypeptide hydrogel prepared in this example on the HepG2 cell activity is shown in Figure 4. Figure 4 shows that the polypeptide hydrogel has no toxic side effects on HepG2 cells, and its activity can reach 100%, indicating that the polypeptide hydrogel has good biocompatibility.
以上所述仅为本发明的较佳实施实例，凡依本发明申请专利范围所做的均等变化与修饰，皆应属本发明的涵盖范围。The above are only preferred implementation examples of the present invention, and all equivalent changes and modifications made in accordance with the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims (8)

  1. 一种自组装形成水凝胶的多肽，其特征在于：所述多肽的氨基酸序列为：Glu-Ile-Trp-Leu-Lys。A self-assembled polypeptide to form a hydrogel, characterized in that the amino acid sequence of the polypeptide is: Glu-Ile-Trp-Leu-Lys.
  2. 如权利要求1所述的一种自组装形成水凝胶的多肽在制备水凝胶中的应用。The use of a polypeptide that self-assembles to form a hydrogel according to claim 1 in preparing a hydrogel.
  3. 一种多肽水凝胶，其特征在于，通过调节权利要求1所述多肽形成的多肽溶液的pH及浓度来进行凝胶化而形成的水凝胶。A polypeptide hydrogel characterized in that it is a hydrogel formed by gelation by adjusting the pH and concentration of the polypeptide solution formed by the polypeptide of claim 1.
  4. 如权利要求3所述的一种多肽水凝胶的制备方法，其特征在于，具体步骤为：将权利要求1所述的多肽以一定pH的PBS缓冲液，配置成一定浓度的多肽溶液，涡旋至多肽粉末溶解，于室温静置凝胶化。The method for preparing a polypeptide hydrogel according to claim 3, wherein the specific steps are: preparing the polypeptide according to claim 1 in a PBS buffer with a certain pH to form a polypeptide solution with a certain concentration, and vortex Spin until the peptide powder dissolves, and let stand at room temperature to gel.
  5. 根据权利要求4所述的一种多肽水凝胶的制备方法，其特征在于：上述制备方法中PBS缓冲液的pH范围为pH 5~8。The preparation method of polypeptide hydrogel according to claim 4, wherein the pH range of the PBS buffer in the preparation method is pH 5-8.
  6. 根据权利要求4所述的一种多肽水凝胶的制备方法，其特征在于：上述制备方法中PBS缓冲液的pH为7。The preparation method of polypeptide hydrogel according to claim 4, wherein the pH of the PBS buffer in the preparation method is 7.
  7. 根据权利要求4所述的一种多肽水凝胶的制备方法，其特征在于：上述制备方法中多肽溶液的浓度范围为7.27~14.54 mM。The method for preparing a polypeptide hydrogel according to claim 4, wherein the concentration of the polypeptide solution in the preparation method ranges from 7.27 to 14.54 mM.
  8. 根据权利要求4所述的一种多肽水凝胶的制备方法，其特征在于：上述制备方法中静置时间为30min。The preparation method of polypeptide hydrogel according to claim 4, wherein the standing time in the preparation method is 30 minutes.
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