WO2020171144A1

WO2020171144A1 - Oral composition using clethra barbinervis containing biofunctional component in form of plant body

Info

Publication number: WO2020171144A1
Application number: PCT/JP2020/006626
Authority: WO
Inventors: 和也秋光; 達博松尾; 何森　健; 明秀吉原; 望月　進; 武二郎高村
Original assignee: 国立大学法人香川大学
Priority date: 2019-02-19
Filing date: 2020-02-19
Publication date: 2020-08-27
Also published as: JPWO2020171144A1; CN113453755A; KR20210127146A; JP6937062B2

Abstract

[Problem] To provide an oral composition that uses a body fat accumulation inhibitory function and/or a total cholesterol increase inhibitory function of a natural plant body, in other words, a plant body in which a rare sugar D-allulose and a rare sugar alcohol allitol, which are produced only in very small amounts in nature, are produced and contained within the plant body. [Solution] This body fat accumulation inhibitory agent or total cholesterol increase inhibitory agent is characterized by containing, as an active ingredient, a plant body containing a rare sugar D-allulose and a rare sugar alcohol allitol, which are produced only in very small amounts in nature, within the plant body, or an extract from the plant body.

Description

Oral composition using Zuina containing a biofunctional component in the form of a plant

The present invention relates to an oral composition using Zuina containing an in-vivo functional ingredient in the form of a plant, more specifically, an oral composition having a body fat accumulation suppressing function and/or a total cholesterol level increase suppressing function. Regarding
Body fat accumulation suppressing function and/or total cholesterol of Zuina or Ryobu (Zuina is called Ryobu depending on the region. In the present invention, Ryobu=Zuina. This description is omitted hereinafter). It utilizes the price increase suppression function.

Due to growing public awareness of health, attempts to maintain health through food that is consumed daily have been attracting attention. In particular, the development of food materials containing dietary fiber containing in-vivo functional ingredients is remarkable. It has often been practiced to dry plants such as leaves and stems into powder and use them as tea and food materials, or to use plants having medicinal properties as powders for health foods. For example, a method of cutting leaves and stems of Moroheiya into appropriate sizes, steaming them with live steam for several minutes, then drying while rubbing and rubbing, and then crushing to obtain Moroheiya powder (Patent Document 1), GABA having a blood pressure lowering effect There is known a method (Patent Document 2) in which a green leaf of a Gramineous plant having a high concentration of is treated with a microwave and dried to obtain a dry powder.

When drying mainly by hot air, the dry powder is discolored to brown, etc., and it does not look good.In many cases, the components are denatured by heat, and the quality is changed due to reduction or disappearance of the content, so the freeze-drying method is adopted. It is often done. For example, succulent plants in the solid state are sterilized and washed using sodium hypochlorite, and when vacuum freeze-dried, the powder manufacturing process of drying at low temperature for a long time ensures that only the colors and ingredients that succulents originally possess (Patent Document 3), a plant belonging to the genus Shishido or Kawaraboufu genus of the Umbelliferae, the Japanese cane such as Touki, Inoki and Hyugatouki, as well as the raw material of any of the so-called ginseng plants. Freeze-dried leaves, stems, and seeds that have been dried in the sun to produce a raw material (Patent Document 4), adding excipients such as starch and a thickener in the freeze-dried state of okra It is known to produce a dry powder such as a method for producing okra tea by making a tablet form or mixing with a substance such as coix or herb tea (Patent Document 5).

JP-A-2003-18975 JP 2002-58446 A JP, 2009-284891, A Japanese Patent Laid-Open No. 2008-303174 Japanese Patent Laid-Open No. 2006-61167 Japanese Patent No. 6437721

With the spread of preventive medicine knowledge, attempts have been made to maintain health by ingesting naturally-derived foods that have physiological activity in a preventive manner in a healthy or semi-healthy state before getting a disease. Has been put on the market as a health food. Many of them have been advocated for their antidiabetic effect, but the actual situation is that few of them have been proven to be effective. As far as the inventors know, rare sugars are harmless natural products that can be added to original foods, do not affect taste, and have not yet been developed as foods and drinks that have safe natural products themselves. .. That is, there has been no report that verifies that the plant itself which does not correspond to the extract from the natural product has a characteristic physiological activity of improving one or more of blood glucose level, weight gain, and visceral fat accumulation. It is considered that there is a strong social demand for a natural product which is excellent in characteristic physiological activity for improving one or more of blood sugar level, weight gain, and visceral fat accumulation and is safe. The present invention provides that plants such as leaves and stems of Zuina or Ryobu contain rare sugars having physiological activity, and the rare sugars improve one or more of blood sugar level, weight gain, and visceral fat accumulation. Based on the fact that it is a characteristic physiologically active substance, it is intended to examine whether or not these plants themselves have the same effects as rare sugars.
The new leaves of deciduous shrubs of the genus Zina belonging to the family Asteraceae were edible, but no nutritionally examined report was found. Therefore, first, rats were fed with a diet to which dry powder of zuina was added to collect basic data on physiological effects of zuina, and whether the same effect as D-allulose (=D-psicose) was obtained or not. The purpose was to examine. Next, in order to elucidate the components of Zuina in more detail, the rare sugars D-allulose and allitol (allitol is a rare sugar of sugar alcohol, so it may be referred to as “sugar alcohol rare sugar”) are examined. However, in particular, it was decided to verify the effect of suppressing body fat accumulation and cholesterol metabolism.

The present invention is a plant body that is a natural product, that is, the plant body itself that contains and produces the rare sugar D-allulose and the sugar alcohol rare sugar allitol that are produced in the natural world in an extremely small amount, and the body fat accumulation. It is an object of the present invention to provide an oral composition that utilizes a suppressive function and/or a total cholesterol level increase suppressing function.

It is known that plants such as leaves and stems of Zuina or Ryobu (hereinafter, may be abbreviated as "Zuina") contain rare sugars having physiological activity and contain rare sugars. In order that Zuina can be used in the form of a plant, the effect of suppressing the body fat accumulation and cholesterol metabolism of Zuina was verified, and the function of suppressing the fat accumulation containing Zina in the form of a plant and/or total cholesterol was investigated. The inventors have invented an oral composition having a function of suppressing increase in value and health foods and drinks containing it.

The gist of the present invention is the following body fat accumulation inhibitors or total cholesterol level elevation inhibitors (1) to (12).
(1) It is characterized by containing a plant or an extract of the plant, which contains and produces the rare sugar D-allulose and the sugar alcohol rare sugar allitol, which are produced in the natural world in an extremely small amount, in the plant. And a body fat accumulation inhibitor or a total cholesterol level elevation inhibitor.
(2) The body fat accumulation inhibitor or total cholesterol level elevation inhibitor according to (1) above, wherein the plant is Zuina or Ryobu.
(3) The body fat accumulation suppressor or the total cholesterol level increase suppressor according to (1) or (2) above, wherein the plant is a plant that has been treated to increase the rare sugar content.
(4) The plant is a bud, leaf, branch, flower or root of a mutant such as an adult tree, an adult tree subjected to D-allulose increasing treatment, and/or a polyploid of any of them, or a germinated seed. The body fat accumulation suppressor or the total cholesterol level increase suppressor according to any one of (1) to (3) above, which is the whole plant grown by passage culture or tissue culture.
(5) The body fat accumulation inhibitor or total cholesterol level elevation inhibitor according to any one of (1) to (4) above, wherein the plant is a dried product.
(6) The body fat accumulation suppressor or the total cholesterol level increase suppressor according to (5) above, wherein the dried product is a freeze-dried product.
(7) The body fat accumulation suppressor or the total cholesterol level increase suppressor according to (6), wherein the freeze-dried product is a freeze-dried product of a liquid nitrogen-immersed frozen body by a freeze-drying device.
(8) The body fat accumulation inhibitor or total cholesterol level elevation inhibitor according to (6) or (7) above, wherein the freeze-dried product is a pulverized product or a crushed product of the freeze-dried product.
(9) The body fat accumulation suppressor or the total cholesterol level increase suppressor according to (8), wherein the crushed product or crushed product is a powdery product.
(10) The body fat accumulation inhibitor according to any one of (1) to (4) above, wherein the plant extract is a water extract or an alcohol extract of the aqueous medium extract residue of the plant. , Total cholesterol elevation inhibitor.
(11) The body fat accumulation inhibitor or total cholesterol level according to any one of (1) to (10) above, wherein the plant or the plant extract is in the form of powder, paste or solution. Rise suppressant.
(12) The body fat accumulation suppressor or the total cholesterol level increase suppressor according to any one of (1) to (11), which is orally administered.

The gist of the present invention is the following oral compositions (13) to (35).
(13) A plant body containing and producing the rare sugar D-allulose and the sugar alcohol rare sugar allitol, which is produced in the plant body in a very small amount in nature, and/or the plant extract The oral composition having the function of suppressing body fat accumulation and/or the function of suppressing increase in total cholesterol level.
(14) The oral composition according to the above (13), wherein the plant is Zuina or Ryob.
(15) The oral composition according to the above (13) or (14), wherein the plant is a plant that has been treated to increase the rare sugar content.
(16) The plant is a bud, leaf, branch, flower or root of a mutant such as an adult tree, an adult tree which has been subjected to D-allulose increasing treatment, and/or a polyploid of any of them, or a germinated seed. The oral composition according to any of (13) to (15) above, which is a whole plant grown by subculture or tissue culture.

(17) The oral composition according to any of (13) to (16), wherein the plant is a dried product.
(18) The oral composition according to the above (17), wherein the dried product is a freeze-dried product.
(19) The oral composition according to the above (18), wherein the freeze-dried product is a freeze-dried product of a liquid nitrogen-immersed frozen body by a freeze-drying device.
(20) The oral composition according to the above (18) or (19), wherein the freeze-dried product is a pulverized product or a crushed product of the freeze-dried product.
(21) The oral composition according to the above (20), wherein the pulverized product or crushed product is a powdery product.
(22) The oral composition according to any of (13) to (16), wherein the plant extract is an aqueous extract or an alcohol extract of the aqueous medium extract residue of the plant.
(23) The oral composition according to any of (13) to (16), wherein the plant or the plant extract is in the form of powder, paste or solution.

(24) The oral composition according to any one of (13) to (23), which further contains a dietary fiber-containing material.
(25) The oral composition according to the above (24), wherein the dietary fiber-containing material is tea powder selected from Japanese tea, black tea and Chinese tea.
(26) The oral composition according to the above (24), wherein the dietary fiber-containing material is green plant powder.
(27) The material according to (24) above, wherein the dietary fiber-containing material comprises one or more of ashitaba, barley leaf, green tea, kale, sweet potato leaf, broccoli, morohaya, sesame seed leaf, mulberry leaf, buttonfish fu, mugwort. Oral composition.

(28) The oral composition according to any one of (13) to (27), which is a food, a quasi drug, or a drug.
(29) The oral composition according to the above (28), which is used as a health food for suppressing body fat accumulation, a health drink or a medicine.
(30) The oral composition according to the above (28), which is used as a health food, a health drink or a drug for suppressing an increase in total cholesterol level.
(31) The above food is prepared as a supplement, a functional food, a health food, an enteral nutrition food, a special-purpose food, a dietary supplement, a food for specified health uses, or a nutritionally functional food. The oral composition according to any of (28) to (30).
(32) The oral composition according to the above (31), wherein the special-purpose food is a food for the sick.
(33) One of the above (13) to (32), which is prepared in the form of tablet, round, capsule, powder, granule, fine granule, troche, or liquid. The oral composition according to.
(34) The oral composition according to the above (33), which is a granule or a tablet.
(35) The food is a beverage, a confectionery, a frozen dessert, a noodle, a processed marine product, a processed livestock product, a dairy product, an oil and fat and an oil/fat processed food, a seasoning, an enteral nutritional food, a soup, a stew, a side dish, a pickle, or The oral composition according to any of (28) to (34) above, which is prepared as bread.

Plants such as leaves and stems of Zuina contain rare sugars having physiological activity, and the rare sugars are characteristic bioactive substances that improve one or more of blood sugar level, weight gain, and visceral fat accumulation. Based on the above, it was expected that Zuina had such a physiological activity even in the form of a plant, but its verification had not been made yet. Rats were fed a diet supplemented with dry powder of Zuina, and basic data on physiological effects of Zuina were collected. As a result, the effects of rare sugar D-allulose on the suppression of body fat accumulation and the increase of total cholesterol It was also found that the influence of the ingredients in zuina was greater. In addition, under high-fat and high-sucrose diet conditions, verification of the anti-obesity effect of dry powder of Zuina, and examination of whether the anti-obesity effect was due to the rare sugars (D-allulose and allitol) in the dry powder of Zuina, were examined. The anti-obesity effect of the powder was confirmed, and the synergistic effect of D-allulose and allitol was confirmed. In the natural world, only a very small amount is produced, and the plant or the plant extract containing and producing the rare sugar D-allulose and the sugar alcohol rare sugar allitol in the plant body suppresses body fat accumulation, or total cholesterol. Expected to suppress price increase.

According to the present invention, the plant itself is a natural product, that is, the plant itself or an extract thereof containing and producing in the plant a rare sugar D-allulose and a sugar alcohol rare sugar allitol which are produced only in a very small amount in nature. It is possible to provide an agent or an oral composition that utilizes the function of suppressing body fat accumulation and/or the function of suppressing increase in total cholesterol level.

FIG. 3 is a diagram showing tissues and inspection items collected in Example 1. 3 is a graph showing changes in body weight of rats in each diet group in Example 1. 5 is a graph showing the body weight and food intake of rats in each diet group in Example 1. 3 is a graph showing relative weights of heart, liver, kidney, and spleen of rats in each diet group in Example 1. 3 is a graph showing the relative weights of perirenal adipose tissue, epididymal adipose tissue, mesenteric adipose tissue, and total intraperitoneal adipose tissue of rats in each diet group in Example 1. 3 is a graph showing total protein, lipase, total cholesterol, and chlor among the serum components of rats in each diet group in Example 1. 4 is a graph showing glycogen, total fat, cholesterol, and neutral fat among the liver components of rats in each diet group in Example 1. 3 is a graph showing carcass fat mass, carcass fat mass, total body fat mass, and total body fat mass of rats in each diet group in Example 1. FIG. FIG. 7 is a diagram showing tissues and inspection items collected in Example 2. 3 is a graph showing the body weight and food intake of rats in each diet group in Example 2. 3 is a graph showing relative weights of heart, liver, kidney, and spleen of rats in each diet group in Example 2. 5 is a graph showing the relative weights of epididymal fat, perirenal fat, mesenteric fat, and total intraperitoneal fat in rats of each diet group in Example 2. 7 is a graph showing carcass fat mass, carcass fat mass, total body fat mass, and total body fat mass of rats in each diet group in Example 2. 5 is a graph showing the concentrations of glucose, insulin and neutral fat, which are serum components of rats in each diet group in Example 2. 5 is a graph showing the concentrations of total cholesterol, HDL cholesterol, and free fatty acids, which are serum components of rats in each diet group in Example 2. Various substances for zuina [1-naphthalene acetic acid (abbreviation NNA), 2,4-dichlorophenoxyacetic acid (abbreviation 2,4-D), 6-benzylaminopurine (abbreviation BAP), sodium chloride (NaCl), D-glucose , Sucrose, D-fructose, D-allose], and the experimental results of examining whether the D-allulose content is increased are shown. In the figure, each treatment concentration is shown on the horizontal axis.

[Plants containing rare sugars]
A plant belonging to the genus Duina is a plant that contains and produces rare sugar D-allulose and sugar alcohol rare sugar allitol, which are produced in the natural world in a very small amount. That is, it is the only plant in the natural world that contains and produces D-allulose and allitol having a physiologically active action called rare sugars. Plants belonging to the genus Duina are sold as seedlings in Japan, and some plants belonging to the species are grown in ornamental gardens due to their long spikes and fragrant head flowers, and are also used as hedges or ikebana. Is used as. Although it is sold as a seedling in Japan, it could not propagate by seed germination. Since it was found that the plants belonging to the genus Duina produce and contain D-allulose and allitol, which have bioactive activities, in the plant body, it is earnestly desired to develop a method for efficiently germinating seeds. became.

The plant of the present invention includes a mature tree, a mature tree that has been subjected to D-allulose increasing treatment, and/or a bud, a leaf, a branch, a flower or a root of a mutant such as a polyploid thereof, or a germinated seed. , Whole plants grown by passage culture or tissue culture. More specifically, for example, plants such as wild and polyploid mutants, plants obtained by cultivation or plants obtained by culture such as tissue culture, for example, leaves, flowers, branches, stems, fruits, Examples include roots, seeds, cultured cells or organs, callus and the like, and various processed products and the like obtained by directly processing these or physically, chemically or biologically.
Examples of the physical/chemical treatment method include drying treatment such as sun drying, air drying, and freeze drying, pulverization treatment with a blender, a homogenizer, a ball mill, and the like.As the physical/chemical treatment product, a dried treatment product, Examples include freeze-dried products and crushed products. Examples of the biological treatment method include a fermentation method, and examples of the biological treatment product include a fermentation treatment product.

In plants belonging to the genus Zuinia, cultivated in a plant culture medium, which is cut from a Zuiina plant body obtained by sexually propagating the wild Zuina (seed breeding), without depending on the tissue culture method of the plant, A method for producing clone seedlings in a good and economical manner has been developed (Patent Document 6).
It is preferable to use buds, leaves, branches, flowers or roots of mature trees, or germinated seeds, plants grown by subculture or tissue culture.

The only plant that contains the rare sugar D-allulose is the genus Duina (Non-Patent Document 1). D-allulose is known to inhibit the growth of many plants, and is also known to have an insulin secretory action (Non-Patent Document 2), an arteriosclerosis-preventing action (Non-Patent Document 3), and the like. .. It has about 70% the sweetness of sugar, but has almost zero calories. In animal experiments with rats, "slow up postprandial blood glucose increase" (Non-Patent Document 4), "suppress accumulation of visceral fat" (Non-Patent Document 5), "hard to become arteriosclerosis" (Non-Patent Document 6) ) Has been reported. However, it has been reported that the effect of suppressing the rise in blood glucose level after eating is affected by the decrease in D-allulose by cooking, and the effect is reduced as compared with the non-heated state. In the case of cooking at high temperature such as karinto, the effect is greatly reduced. It has been confirmed that it inhibits the growth of nematodes and may be used as an anthelmintic drug. The present invention relates to a plant powder containing a rare sugar, which is produced by freeze-drying and pulverizing a plant of Zuina or Ryob. A powder which can be used as a raw material for food or a health-promoting agent by making it possible to easily use rare sugars contained in these plants and by applying it to a wide range of applications Is. It has never been verified that the rare sugar containing Zuina even in the form of a plant has a characteristic physiological activity of improving one or more of blood sugar level, weight gain, and visceral fat accumulation. For the first time, the anti-obesity effect of Zuina dry powder was verified under the condition of high fat and high sucrose diet. The anti-obesity effect was also due to the rare sugar (D-allulose) and allitol in the dry powder of Zuna, and the synergistic effect of D-allulose and allitol could be confirmed. In the natural world, only a very small amount is produced, and the plant or the plant extract containing and producing the rare sugar D-allulose and the sugar alcohol rare sugar allitol in the plant body suppresses body fat accumulation, or total cholesterol. It is expected that a price increase suppression effect will be expected.

[Plants that have been treated to increase the content of rare sugars]
In order to produce plants that have been treated to increase the content of rare sugars, the present inventors have found that various substances [1-naphthalene acetic acid (abbreviated as NNA) auxin-like plant growth regulator, 2 ,4-Dichlorophenoxyacetic acid (abbreviation 2,4-D), a plant hormone agent with an auxin-like action, 6-benzylaminopurine (abbreviation BAP), a first-generation synthetic cytokinin that stimulates plant growth, sodium chloride (NaCl ), D-glucose, sucrose, D-fructose, D-allose], and examined whether the D-allulose content increased.
Cultured individuals about 2 months after passage were used and tested in a growth chamber (normal light condition: 100-150 μmolm ⁻² S ⁻¹ ). Hydroponics was carried out for 3 days under the condition of 28° C. and each treatment was carried out for 10 days. Hydroponics was carried out by floating the cultured plant individual in pure water, and sugar treatment was performed by changing the sugar solution every two days. The sampled leaves were frozen under liquid nitrogen, crushed into powder, and then extracted with sterile water. D-fructose and D-glucose were removed by adding dry baker's yeast to the above sugar extract. The amount of D-allulose in this rare sugar solution was quantified by the cysteine-carbazole sulfate method. The results are shown in Fig. 16. In the figure, each treatment concentration is shown on the horizontal axis. As shown in FIG. 16, it was revealed that the amount of D-allulose in Zuna fluctuates greatly by performing various treatments. There are those that decrease by pretreatment and those that increase on the contrary. In particular, it is very useful to increase the D-allulose content in zuina by performing a pretreatment that can increase the D-allulose content of the plant by sugar treatment.

The above results were obtained, and the D-allulose content of leaves by sugar (monosaccharide/disaccharide) treatment under light and dark conditions was experimentally examined and the results are shown in Table 1. The data values in Table 1 are relative values with the D-allulose content determined using the cysteine-carbazole-sulfuric acid method as 100% for untreated plots under light conditions (100% for untreated plots under dark conditions). Indicated. In the experiment, cultured individuals about 2 months after passage were used and tested in a growth chamber (100-150 μmolm ⁻² S ⁻¹ ). It was carried out under a condition of 28° C. for 3 days of hydroponic culture (ordinary light) and 2 days of sugar treatment (ordinary light condition/complete light-shielding dark condition). In hydroponics, individual cultured plants were floated on pure water and sampled 3 days after the start of sugar treatment (unlike the above, no sugar solution exchange). The sampled leaves were frozen under liquid nitrogen, crushed into powder, and then extracted with sterile water. D-fructose and D-glucose were removed by adding dry baker's yeast to the above sugar extract. The amount of D-allulose in this rare sugar solution was quantified by the cysteine-carbazole sulfate method. Each sugar was treated so as to have a concentration of 10 mM.

The data values in the table are the relative value (relative value (%)) of the D-allulose content quantified using the cysteine-carbazole sulfate method, with the untreated section under light conditions being 100%, and under light and dark conditions. It was shown as a relative value (relative value (%) <each untreated ratio>) with the untreated section in 100% as 100%.

Moreover, it was confirmed by experiments that the contents of D-allulose and allitol can be increased by artificial multiplication of chromosomes.
In the experiment, leaf sugar contents in diploid and tetraploid under light and dark conditions were examined, and the results are shown in Table 2. All the data values in Table 2 are each sugar content (mg/g dry weight) per 1 g of dry weight of leaves.
The D-psicose content was shown as a relative value, with the untreated plot under bright conditions being 100% (the untreated ratio is 100% for untreated plots under dark conditions). Subcultured individuals of Zuina, a diploid, were treated with 125 μM amiprophos-methyl (APM) for 24 hours, and subcultured and analyzed for ploidy using flow cytometry. It was unified and a tetraploid individual was created. In addition to amiprophos-methyl, for example, colchicine or the like can be treated for chromosome doubling.
Cultured individuals about 2 months after passage were used and tested in a growth chamber (100-150 μmolm ⁻² S ⁻¹ ). It was carried out under a condition of 28° C. for 3 days of hydroponic culture (ordinary light) and 3 days of light/dark treatment (ordinary light/dark condition of complete shading). The leaves were sampled, lyophilized for 2 days, weighed, and the monosaccharide content was measured. The sugar content was calculated from the HPLC peak area and the calibration curve of the standard sample. Experiments were performed with n=4 for diploids under bright conditions and n=5 for other conditions.

Examples of plant extracts include extracts obtained from the above-mentioned plants by various extraction methods. Examples of the extraction method include various solvent extractions and supercritical fluid extractions. The extract is sedimentation separation, cake filtration, clarification filtration, centrifugal filtration, centrifugal sedimentation, compression separation, various solid-liquid separation methods such as filter press, various concentration methods, various drying methods, formulation methods such as granulation or powderization, You may process by various purification methods etc. Examples of the purification method include a solvent fractionation method, a column chromatography method and a recrystallization method. Concentration and drying methods include freeze drying, natural drying, hot air drying, ventilation drying, blast drying, spray drying, reduced pressure drying, sun drying, vacuum drying, fluidized bed drying, foam layer drying, drum drying, and other film drying methods. , Ultrasonic drying, electromagnetic wave drying and the like, preferably spray drying and freeze drying.
Upon extraction and treatment of the extract, for example, an antioxidant and a preservative can be added. As the solvent used for solvent extraction, any solvent can be used as long as it can extract the functional components of the plant, for example, water, distilled water, deionized water, an aqueous solution of an inorganic salt, an aqueous medium such as a buffer, methanol, ethanol, Monohydric alcohols such as propanol and butanol, polyhydric alcohols such as propylene glycol and glycerol, hexane, toluene, petroleum ether, benzene, ethyl acetate, chloroform, dichloromethane, 1,1,2-trichloroethene, dimethyl sulfoxide, acetone And the like, and an organic solvent and the like are preferable, and an aqueous medium and alcohol are preferable.
Examples of the buffer solution include a phosphate buffer solution and a citrate buffer solution. Examples of the inorganic salt in the aqueous solution of inorganic salt include sodium chloride, potassium chloride, calcium chloride and the like. The alcohol is preferably a monohydric alcohol, and the monohydric alcohol is preferably ethanol.
These solvents can be used alone or in combination of two or more. The mixed solvent is preferably hydrous alcohol, more preferably hydrous monohydric alcohol, and particularly preferably hydrous ethanol. The water content is preferably 70% or less, more preferably 40% or less. As the solvent, carbon dioxide converted into a supercritical fluid can also be used.
Extraction is performed using, for example, 0.1 part by weight to 10000 parts by weight of solvent, preferably 1 part by weight to 100 parts by weight, relative to 1 part by weight of the plant. The extraction temperature is not particularly limited, but is preferably 0°C to 100°C, more preferably 20°C to 90°C. The extraction time is not particularly limited, but is preferably 1 minute to 1 week, more preferably 30 minutes to 1 day. Further, as a method for extracting from Zuina or Ryoubu, the above-mentioned Zuina or Ryobu, the physical/chemically processed product of the above-mentioned Zuina or Ryobu, the residue obtained by extracting the biologically-processed product with an aqueous medium is water, alcohol, or water-containing material. It can be extracted with alcohol.
The aqueous medium is not particularly limited, but water, pure water and deionized water are preferable. The extraction temperature when extracting with an aqueous medium and alcohol or hydrous alcohol is not particularly limited, but is preferably 0°C to 100°C, more preferably 20°C to 90°C. The extraction time is not particularly limited, but is preferably 1 minute to 1 week, more preferably 30 minutes to 1 day. In addition, it is preferable to use dried or fermented Zuina or Ryobu.
The equipment used for extraction is not particularly limited, but examples thereof include a container, a stirrer, a reflux condenser, a Soxhlet extractor, a homogenizer, a shaker, an ultrasonic generator, etc. devised for efficient extraction. The liver function-protecting agent or improving agent of the present invention contains Zuina or Ryobu or the extract prepared by the above-mentioned method, and if necessary, one or more pharmaceutically acceptable carriers, and further if necessary. It may optionally contain other therapeutically active ingredients.

For example, it is known that duina leaves contain about 5% of raw weight of D-allulose among rare sugars. In the present invention, for the purpose of utilizing the rare sugars contained in these plant matter, it contains rare sugars by powdering plants including leaves, shoots, stems, roots, etc. It can be used as a powder. Collection of plants from Zuina or Ryobu is, for example, collection of leaves from mature trees or mutants such as mature trees and polyploids of which D-allulose has been increased, or seedlings germinated from seeds, tissue culture. Alternatively, a powder can be obtained by collecting and freeze-drying the whole plant body including leaves, stems, and roots of the subcultured seedlings.
In the present invention, commercially available freeze-drying equipment is used for freeze-drying the Zuiina plant. Compared with other drying methods, the freeze-drying method is a low-temperature treatment, so that it has no nutrient destruction and is excellent in reconstitution. The prevention of tissue destruction due to dehydration is also a feature of this drying method. Since food is close to 90% water, great care must be taken against spoilage. Freeze-drying is used for the production of preserved foods because the final dryness is much better than other drying methods. In addition, since the weight of the dried food is reduced by the amount of water removed, it is suitable for transportation. A powder of a plant of Zuina or Ryob is produced from a plant of Zuina or Ryob without impairing the functional components of the plant of Zuina or Ryob as much as possible. For example, a step of crushing a plant of Zuina or Ryob to form a slurry, a step of pre-freezing the slurry into a divided freezing type, and a vacuum freeze-drying treatment for reducing the water content of the frozen product to 3 to 5% It is characterized by comprising a step and a step of adjusting the powder of the dried product obtained from the step. Further, vacuum drying or spray drying is applied as the drying treatment.

The cultivation and freeze-drying of Zuina will be described below in detail.
As plants containing rare sugars, zuna and ryobu are known at present, and the following description is made according to a plant pictorial book.
[Zuina] Zuina is a plant belonging to the genus Zuina. The genus Duina (genus Duina, genus Venezuelan, scientific name: Itea spp.) is a deciduous shrub of the family Asteraceae, and is a genus of plants consisting of about 10 species of shrubs. The young leaves are boiled and eaten like yomena, so it is also called Japanese linden. The trunk is 1-2 meters in height, the young branches are pale green, the leaves are alternate, the oval oblong shape is sharp, and the tips are sharp and the edges are fine. There are saw teeth. On the leaf surface, about 8 pairs of parallel lateral veins are conspicuous. In May-June, the shoots are inflorescences 5-17 cm long, and many white florets open. The petals are ovate lanceolate, five erect, with the same number of petals and stamens as petals, one pistil, stigma head, and ovary semi-upper. Zuirina grows in the mountains of warm regions and is distributed in the southern Kinki region, Shikoku, and Kyushu. The genus Duina is known to be the only plant that produces D-allulose and allitol contained in rare sugars in the plant. It is also sold as a seedling in Japan.

[Ryobu]
Ryobu is a plant that belongs to the family Ryobiaceae, and is described in the botanical pictorial book as follows.
Distributed on Hokkaido, Honshu, Shikoku, Kyushu, and Jeju Island in the Korean Peninsula, it is a small deciduous tree that grows in the forest. It has a height of 3 to 7 meters, its trunk is smooth and brown, and its branches are ring-shaped. Young shoots have stellate hairs. The leaves are pedunculated, alternate, broadly inverted needle-shaped, with serrated edges, clustered at the tips of branches, sparsely hairless or stellate hairs, and dense hairs on the dorsal veins. In summer, put out racemes 6 to 15 cm in length on the tips of the branches and attach small white flowers densely. The sepal is small and splits into five. The corolla is 5-6 mm in diameter and has 5 deep splits, resembling a small plum blossom. 10 stamens, 1 pistil. It is a spherical and small fruit. The wood becomes fine charcoal, and the young shoots are edible. Ryobu does not contain the rare sugar allulose, but depending on the region, Zuina is sometimes called Ryobu. The present invention relates to Zuina.

[Cultivation from zuina seeds]
Since the seeds of Zuina are dormant, they will not germinate even if they are sown unless the dormancy is broken. In order to break the dormancy of seeds, it is necessary to store the seeds in a cool, dark place in a dry atmosphere for a predetermined period of time. It is done by storing for a period. As for the drying condition, it is preferable that the water vapor pressure is low, and for example, it is stored together with a large amount of silica gel in a closed plastic container. The storage temperature is 4°C or lower, preferably 4°C. In addition, the storage period under such conditions is preferably 2 weeks or more, more preferably 2 to 6 months. Since the seed of Zuina is a light germinated seed, it is difficult to germinate it by covering it with soil, and it is preferable to germinate it in a bright place at a temperature of 25 to 30°C. The germination rate after 2 weeks at 28°C is about 60%. Is.
In order to grow germinated seeds, for example, the germinated seeds are grown in the same 1/2 MS medium [containing 1% sucrose (pH 5.8)] or planted in rock wool or soil in the same manner as ordinary plants. Can grow. Whether sprouted seeds were planted in a medium or soil, the growth speed was the same after that, but the soil cultivation slightly promotes lignification. Compared with these, in the rock wool cultivation, the growth in the early cultivation is faster. The germinated seeds can be transplanted, for example, about 10 days after sowing.

[Growth by tissue culture]
Seedlings in which the 2nd to 5th leaves of Zuina were developed, or the Zuina plant body containing the shoot apex or the stem of a normal Zuoina juvenile tree was left as it was, or the leaves were removed as it was, and 1/2MS medium (1% sucrose was used). There is a method of cultivating Zuina which makes it possible to proliferate a large amount of seedlings simply by placing them in a plant culture medium (with or without addition) or by piercing and culturing them like cuttings. As the Zuina plant, a seedling obtained by breaking down the dormancy of seeds improved by crossing can be used. Mass production of seedlings by germination of seeds that have broken dormancy and subculture thereof makes it possible to easily obtain a large number of plants or seedlings of Zuina.

[Growth of Duina seedlings by subculture]
In order to mass-produce the seedlings of Zuina, a part of the plant can be taken out and aseptically cultured in a test tube or the like by tissue culture. For example, a large amount of seedlings can be easily produced by culturing cells near the growth point at the tip of the stem. For example, a plant body (about 5 to 10 mm) containing the shoot apex of the seedling in which the 2nd to 5th leaves have spread is cut out, and it is cultivated by cutting it into a 1/2 MS medium and piercing it like a tree, or placing only the stem. A plurality of side shoots are observed from the lower part (plant including roots and cotyledons or first leaves) of the plant with the shoot apex cut. It is possible to easily obtain the growing point because the side shoots are formed by cutting the shoot apex. In the case of stems without leaves only, homogeneous young leaves from all can be observed by simply placing them in the medium.

When 1/2 μM medium was added with 3 μM, 5 μM, 10 μM indolebutyric acid (IBA)-containing medium and non-added medium were prepared, and cuttings were carried out. Roots were induced. However, since roots were formed earliest in the additive-free medium and the roots grew well, it is considered that plant hormones (IBA etc.) are not particularly necessary.
For the subculture, either cuttings on the shoot apices or cuttings on the shoots (excluding the shoot apices) may be used, and rooting is generally performed within 10 days after transplantation to 1/2 MS medium (containing 1% sucrose). To do. In the case of only stems without leaves, rooting is observed in some of the homogeneous young leaves and a part of them after 30 days just by placing them in the medium.

[Zuna seedling making]
A plant rooted in a petri dish (cultivated until rooted for one month or more) is transplanted to vermiculite or culture soil, and preferably covered with a hood such as a transparent film. It is preferable to use vermiculite rather than the culture soil because the growth tends to be suppressed when the culture soil is used during the culture from the seedlings. Then, after 2-3 days, the hood is shifted to remove moisture, and after 2-4 days, the hood is completely removed. With regard to this acclimatization step, if a container with a hood is used, even seedlings of only Futaba will not cause any problems. Further, it is possible to raise seedlings by hydroponics using rock wool or the like. During the seedling raising period, it is preferable to cover the larvae of Spodoptera litura by covering them with insect repellent nets. The rooted Zina plant is transplanted to a medium or culture soil in a plug tray and grown in the form of a plug seedling.
If the outside temperature becomes low during the growth (the minimum temperature is below 15 degrees), the leaves turn red. Zuina does not fall, but Kobanozuina leaves in winter. Since the growth of seedlings is greatly affected by temperature, it is more efficient to start the acclimation step after early spring and grow it for the summer, and in winter, concentrate on the above-mentioned passage work and grow seedlings from the early spring. The schedule to get to is good.

[Growth from seedlings of Zuina]
As in the case of making Zuina seedlings, Zuina plants containing stems were cut from a young tree of Zuina, or Zuina plants containing shoot apices or stems were cut from seedlings in which the 2-5th leaves of Zuina were expanded. Zuina plants as they are, or leaves removed, as they are, placed in rooting medium and pierced like a tree, cultivated and rooted, and the rooted Zuina plants are cultivated in medium or culture soil, cultivation materials (rock wool). Etc.) and grow the young leafy vegetables and/or fresh leaf vegetables until they can be harvested, and the young leafy vegetables and/or fresh leaf vegetables are harvested. As the rooting medium, 1/2 MS medium or Gamborg B5 medium is used. By using a medium when growing rooted Zuina plants into Zina vegetables, it is possible to aseptically grow functional vegetables based on the functions of D-allulose and allitol.

[Zuina freeze-drying]
(When using whole Zuna seedlings aseptically cultivated in a petri dish)
The rootling medium (for example, MS medium) was removed as much as possible using a toothpick or the like to remove the roots of submerged Zuinia seedlings (48 days) by hand so as not to cut the roots. Then, the water is wiped off, and the fresh weight of the seedlings of Zuinia japonica is measured. In this case, about 1 to 4 g of Zuina fresh weight can be obtained for one petri dish. For example, Table 3 is a table showing an example of the measurement results of the number of petri dishes and the fresh weight of the Zuna seedlings.

(When using only the grown leaves of Zuina grown in the field)
Only the grown leaves of Zuina grown in the field are cut with pruning shears and collected in a bag. Then, the fresh weight of the dried leaves of Zuina is measured.
The leaves of Zuina are soaked in liquid nitrogen and frozen, then placed in a double bag and stored at -80°C. Crush the preserved adult leaves of Zuina.

(Common procedure)
Dip the zuina in liquid nitrogen to freeze it, put the frozen zuina in a stainless beaker cooled with liquid nitrogen, and then cover the beaker with double gauze. A 5 liter beaker containing zuina was placed in a vacuum freeze dryer (FREEZONE 12Plus, LABCONCO), or a 2 liter beaker containing zuina was placed in a vacuum freeze dryer (FREEZE DRY SYSTEM/FREEZONE 2.5, LABCONCO). Manufactured) and freeze-dried for a certain time with occasional stirring. Even when the zuina was put in a 5 liter beaker, it dried in about one week. After drying, the weight was measured to be about 1/10 of the fresh weight. The weighed Zuina was transferred to a mini speed mill (RaboNext). After crushing the zuina for 10 to 20 seconds with a mini speed mill, the contents were confirmed, and this was repeated until the zuina became powdery. If the zuina is continuously crushed, the temperature of the mill becomes high, and the zuina powder is discolored. Therefore, it is preferable to cool the mill or replace the mill. The crushed zuina was collected as much as possible into a container using a spatula and a spatula. The lid of the container containing the zuina was wrapped with parafilm, shielded from light with aluminum foil, placed in a box, and stored at room temperature in the dark.

[Orally administered body fat accumulation inhibitor or total cholesterol level elevation inhibitor, or oral composition]
The body fat accumulation inhibitor or total cholesterol level elevation inhibitor orally administered orally according to the present invention, or oral composition (hereinafter collectively referred to as “oral composition”), is intended for mammals including humans, Orally administered to the mammal. The oral composition of the present invention is a composition having a body fat accumulation suppressing function and/or a total cholesterol level increase suppressing function, and may be foods, pharmaceuticals, food additives and the like. The oral composition of the present invention utilizes, in the form of a plant, a deciduous shrub of the Yukinoshita family, which is a plant containing and producing allulose and allitol, which are produced only in a very small amount in nature in the plant. In the past, although no reports have been found that were nutritionally examined, it is a plant itself that uses new leaves, so it can be reasonably ingested on a daily basis, so-called functional foods. Suitable for use as a food such as.

As described above, the oral composition of the present invention contains zuina as a plant form or as a plant extract, but in addition to it, various health ingredients, and various additives acceptable as foods or pharmaceuticals. It may be included. Other components include various carriers that are pharmaceutically or food hygienically acceptable as orally administered agents, such as excipients, lubricants, stabilizers, dispersants, binders, diluents, flavors, sweeteners, Flavoring agents, coloring agents and the like can be exemplified. The form of the composition of the present invention is not particularly limited as long as it exhibits the effects of the present invention, and examples thereof include tablets, pills, granules, fine granules, chewable agents, capsules, liquid agents, chewable agents, and beverages. Etc. It may be in the form of other foods. These dosage forms can be prepared using conventional methods commonly known in the art.

For example, when the oral composition is formed into granules, the granulation method is not particularly limited, and the oral composition can be formed by any method such as a fluidized bed granulation method and an extrusion granulation method. Above all, a fluidized bed granulation method that can obtain a granulated product having good dispersibility can be preferably used. Usually, when granulating a powdery oral material, a binder such as hydroxypropyl cellulose, hydroxypropylmethyl cellulose, methyl cellulose, carboxymethyl cellulose, pullulan, starch, dextrin, guar gum, galactomannan is used.
The obtained granular composition has good dispersibility in water, has a small tendency to scatter as a fine powder, and can be easily packaged as a product. In addition, generally, for health and taste reasons, an additive other than a plant, such as zuina, which is an in-vivo functional ingredient, is often added to the consumer by adding a lot of binders such as the above hydroxypropylcellulose. Not liked by. The binder can be used in an amount within the range that does not impair the solubility and dispersibility when the obtained granular composition is dissolved in water. Depending on the strength of the binder used, the binder can be used in an amount of 3% by weight or less when used as a solution, and can be used in an amount of 10% by weight or less when added as a powder.

[Rare sugar]
Rare sugars are defined as monosaccharides and their derivatives that are present only in trace amounts in nature. For hexose, in the case of aldose, L-allose, L-gulose, L-glucose, L-galactose, L-altrose, L-idose, L-mannose, L-talose, D-talose, 16 types of D-mannose, D-idose, D-altrose, D-galactose, D-glucose, D-gulose, D-allose, L-allulose, L-sorbose, L-fructose, L- for ketose There are eight types, tagatose, D-tagatose, D-fructose, D-sorbose, and D-allulose. For example, D-allulose has not been reported to have toxicity to humans, and toxicity to animals is considered to be low.
As a method for obtaining D-allulose with a rare sugar, a production method obtained by treating D-fructose with an enzyme (epimerase) is generally used at present.

The rare sugar D-allulose has the properties as an assimilating sugar, an excellent sweetener, an antiobesity agent, an antifeedant, an insulin sensitizer, and a low-calorie sweetener as described above, and even in low-calorie Nevertheless, it has new characteristics such as suppressing food intake. In addition, since it has a sweetness similar to that of sugar and has a low calorie, it is attracting attention as a sugar that can be widely used as a low-calorie sweetener. Rare sugars are monosaccharides excluding D-glucose and D-mannose that are abundant in nature, for example, D-allulose, D-allose, which is a monosaccharide that produces only a very small amount in nature, The properties and uses are still under development. At present, rare sugars such as D-allulose can be produced using enzymes and the like, but it has been found that they are also contained in plants such as leaves of Zuina and Ryob.
Rare sugars are defined by the International Society for Rare Sugars as "monosaccharides and their derivatives that are abundant in nature", and when monosaccharides and sugar alcohols as their derivatives are added, there are about 60 types. D-Glucose, D-fructose, D-mannose, and D-galactose that can be produced from lactose in milk are abundant in nature, and other substances are classified as rare sugars that exist only in trace amounts. The discovery of DTE has made it possible to produce D-fructose, D-allulose, and further D-allose, allitol, D-talitol from D-glucose.

Allitol is a rare sugar alcohol sugar and is a polyol (sugar alcohol). Specifically, allitol is a sugar alcohol having 6 carbon atoms produced by reducing D-allulose, and it is considered that it exists in zuina while dynamically moving back and forth through redox with D-allulose. .. In the present embodiment, by using Zuna, which is a plant body containing D-allulose and allitol, it is intended to elucidate the physiological function of the plant body containing both D-allulose and allitol. There are many unclear points regarding physiological functions, and it is considered that they will be revealed in future studies.

[Materials containing dietary fiber]
The oral composition of the present invention may further include a dietary fiber-containing material. A preferred example of the dietary fiber-containing material is tea powder selected from Japanese tea, black tea and Chinese tea.
The oral composition of the present invention may be in the form of tablets, capsules, granules, powders, troches, or the like, and therefore, for example, a dietary fiber-containing material similar to the pulverized or crushed product of freeze-dried Zuna is added. Even in the case, the granulation can be performed well. Many studies have been conducted on the main actions of dietary fiber in the fields related to protein metabolism, lipid metabolism, and carbohydrate metabolism. In addition, research on physiological effects on bacterial groups present in the intestine has also attracted attention. Dietary fiber is mainly contained in leaves, buds, stems, flowers, fruits, roots, ears, seeds, fruits and the like, which mainly contains plant fibers. The dietary fiber-containing material is typically a material containing green plant fibers. Preferred examples include food materials for producing so-called green juice. Examples thereof include ashitaba, barley young leaves, green tea, kale, sweet potato young leaves, broccoli, moloheiya, sesame young leaves, mulberry leaves, button-bowhu, mugwort and the like, and mixtures thereof. The shape and manufacturing method of the dietary fiber-containing material are not particularly limited, but are preferably powdery, and preferably powder of 75 μm or less.

[Body fat accumulation suppression function and total cholesterol level increase suppression function]
As shown in Examples 1 and 2 described later, the inventor has found that the dried powder of Zuina has a body fat accumulation suppressing function and a total cholesterol level increase suppressing function. Such a physiological action was recognized in rare sugars extracted from natural products, but in the present invention, even Zuina dry powder which is not an extracted rare sugar has a function of suppressing body fat accumulation and a function of suppressing increase in total cholesterol level. I found out. This suggests that the dry powder itself can be used as a raw material of an agent having a body fat accumulation suppressing function and a total cholesterol level increase suppressing function.

In addition to the above, the oral composition of the present invention may contain additives or auxiliary components generally used in foods or pharmaceuticals. For example, starch, lactose, crystalline cellulose, maltitol, dextrin, pullulan, guar gum as an excipient, aspartame, sucralose, acesulfame K as a sweetener, high-sweetness sweeteners such as stevia, monatin, monerin, miraculin, sorbitol, xylitol. , Mannitol, erythritol, lactitol, maltitol, and other sugar alcohols, and sweeteners such as ketose and aldose may be further mixed to adjust the sweetness. Carotene, L-ascorbic acid, α-tocopherol, lutein may be used as a strengthening agent. , Lycopene, etc. may be included.

The dosage form of pharmaceuticals (including quasi drugs) may be any of injections, suppositories, inhalants, transdermal agents, various external preparations, tablets, capsules, granules, powders, syrups, etc. The dosage form may be oral administration (internal use) or parenteral administration (external use, injection). To prepare pharmaceutical formulations of various dosage forms as described above, for example, the rare sugar D-allulose and sugar alcohol rare sugar allitol, which are produced in a very small amount in the natural world of the present invention, are produced and contained in the plant body. Plants or other pharmaceutically acceptable excipients, binders, fillers, disintegrants, surfactants, lubricants, dispersants, buffers, preservatives, flavoring agents, flavors, coatings Agents, carriers, diluents, other medicinal components and the like can be used in an appropriate combination.

Examples of the medicinal component that can be incorporated into the above-mentioned pharmaceutical products (including quasi drugs) include vitamins, drugs known to have a fat metabolism promoting action, and natural products. That is, in addition to the above, the oral composition of the present invention includes vitamins, vitamin-like substances, proteins, amino acids, oils and fats, organic acids, carbohydrates, plant-derived raw materials, animal-derived raw materials, microorganisms, food additives, and pharmaceuticals. Orally ingestible ingredients such as additives can be appropriately contained.

When a plant containing the rare sugar D-allulose and sugar alcohol rare sugar allitol produced and contained in the plant, which produces only a very small amount in nature, is used as a food, it can be carried out by a usual method. .. Specifically, for example, it can be carried out as described below. As a food, a plant containing the rare sugar D-allulose and the sugar alcohol rare sugar allitol produced in the plant or containing the plant extract, which is produced in a very small amount in nature, and the animal is orally As long as it can be ingested, there is no particular limitation on the type and shape of food. Examples of the food include confectionery such as drops, candies, ramunes, gummy, chewing gum; Western confectionery such as cookies, crackers, biscuits, potato chips, cakes, chocolates, donuts, puddings and jellies; rice crackers, yokan, daifuku, ohagi, Japanese sweets such as buns and castella; frozen desserts such as ice cream, ice candy, sorbet, gelato; breads such as bread, French bread, croissants; noodles such as udon, buckwheat, Chinese noodles, kishimen; kamaboko, chikuwa, fish sausage, etc. Fish meat paste products; ham, sausages, hamburgers, corn beef and other meat products; salt, pepper, miso, soy sauce, sauces, dressings, mayonnaise, ketchup, sweeteners (eg sugar, honey, candy, candy, jam, marmalade, etc.) ), spices (for example, mustard, pepper, etc.); seasonings such as Akashi-yaki, Takoyaki, Monja-yaki, Okonomiyaki, yakisoba, yaki-udon, etc.; dairy products such as cheese, butter, margarine, yogurt; Natto, deep-fried tofu, tofu, konjac, dumplings, pickles, boiled gyoza, choux mai, croquettes, sandwiches, pizza, hamburgers, salads, and other various livestock products; beef, pork, chicken, and other livestock products; prawns, scallops, shrimp, Examples include seafood such as kelp; various powders obtained by powdering vegetables/fruits, plants, yeasts, algae, etc.; solidified oils/flavors (vanilla, citrus fruits, bonito, etc.); beverages and the like.

Beverages include food and drink such as soups and miso soups; powdered foods and drinks such as instant coffee, instant tea, instant milk, instant soup and instant miso soup; whiskey, bourbon, spirits, liqueur, wine, fruit wine, sake, Chinese sake, Alcoholic beverages such as shochu, beer, non-alcoholic beer having an alcohol content of 1% or less, sparkling liquor, and shochu; beverages containing fruit juice (for example, apple, mandarin orange, grape, banana, pear, plum juice, etc.), vegetable juice (for example, , Tomato, carrot, celery, cucumber, watermelon vegetable juice, etc.) beverage, fruit juice and vegetable juice beverage, soft drink, milk, soy milk, milk beverage, drink type yogurt, coffee, cocoa, tea beverage (black tea, Green tea, barley tea, brown rice tea, sencha, gyokuro tea, houjicha, oolong tea, turmeric tea, puerh tea, rooibos tea, rose tea, chrysanthemum tea, herbal tea (eg, mint tea, jasmine tea), nutritional drink, sports Beverages, non-alcoholic beverages such as mineral water, etc. may be mentioned.

Preferred examples of such food composition include jelly, tea beverage, alcoholic beverage, drop, candy, ramune, cookie, cracker, biscuit, chocolate, cheese, butter, margarine, chewing gum and the like.

Further, the food of the present invention is prepared as a functional food, a health food, a food for specified health use, a food with a health function such as a food with a nutritional function, a special-purpose food (for example, a food for the sick), a dietary supplement, a supplement, etc. However, it is preferable that it is prepared as a food for specified health use, a food for special purpose, a dietary supplement or a supplement.

Examples of the shape of such foods include tablets, rounds, capsules (including hard capsules, soft capsules and microcapsules), powders, granules, fine granules, troches, liquids (syrups, milks, suspensions, etc.). (Including turbidity) and the like, and tablets and capsules are preferable.

The food of the present invention is preferably a tablet-shaped or capsule-shaped food for specified health use, a special-purpose food, a dietary supplement, or a supplement. Here, the supplement means not only a dietary supplement or the like for supplementing nutrients, but also a function useful for maintaining, recovering, improving health (for example, suppressing weight gain, suppressing body fat accumulation, etc.). A health functional food having an obesity effect or a slimming effect) is also meant.

The food of the present invention, for example, a plant or a plant containing the rare sugar D-allulose and the sugar alcohol rare sugar allitol produced in the plant, which is produced in the food in a very small amount in the natural world by a known method. It can be produced by adding a body extract. Specifically, for example, a tablet-shaped food product, which produces only a very small amount in nature, contains a rare sugar D-allulose and a sugar alcohol rare sugar allitol, which is produced and contained in the plant body, and shaped. It can be manufactured by adding raw materials such as an agent (for example, lactose, sucrose, mannitol, etc.), a sweetening agent, a flavoring agent and the like, mixing them, and applying pressure with a tableting machine or the like to form tablets. If necessary, other materials (for example, vitamins such as vitamin C, minerals such as iron, dietary fiber, etc.) can be added. The capsule-shaped food composition contains, for example, a plant or a plant extract containing and producing the rare sugar D-allulose and the sugar alcohol rare sugar allitol in the plant, which produces only a very small amount in nature. It can be produced by filling a capsule with the liquid, suspension, paste, powder or granular food composition, or encapsulating with a capsule base.

The food of the present invention, in addition to the commonly used food materials, food additives, various nutrients, vitamins, flavor substances (for example, cheese, chocolate, etc.), etc., physiologically unless the effects of the present invention are impaired. An acceptable carrier or the like can be added. As the physiologically acceptable carrier and the like, various conventional organic or inorganic carrier substances are used, and excipients, binders, disintegrants, lubricants, colorants, sweeteners, preservatives, antioxidants, Examples thereof include thickeners and emulsifiers. Examples of food additives include colorants, sweeteners, preservatives, antioxidants, and flavoring agents. Furthermore, other materials, for example, minerals such as iron, dietary fiber such as pectin, carrageenan, and mannan may be contained.

Excipients, binders, disintegrants, lubricants, solvents, solubilizers, suspending agents, buffers, thickeners, colorants, sweeteners, preservatives, and antioxidants are as described above. The same thing as what is used for the formulation of this invention is mentioned.

The vitamins may be water-soluble or fat-soluble, for example, retinol palmitate, tocopherol, bisbentamine, riboflavin, pyridoxine hydrochloride, cyanocobalamin, sodium ascorbate, cholecalciferol, nicotinic acid amide, pantothene. Calcium acid, folic acid, biotin, choline bitartrate and the like can be mentioned.

For tablet-shaped, granular, fine-grained food compositions, etc., coating with a coating base material by a method known per se for the purpose of masking taste, improving photostability, improving appearance or enteric properties, etc. You may. Examples of the coating substrate include those similar to those used in the above-mentioned formulation of the present invention, and the coating can be carried out in the same manner.

Health products such as dietary supplements, foods for specified health use, etc. containing plants or plant extracts that contain and produce rare sugar D-allulose and sugar alcohol rare sugar allitol, which are produced in the natural world in very small amounts. When added to special-purpose foods such as functional foods and foods for the sick, it produces only a very small amount in nature, and contains rare sugar D-allulose and sugar alcohol rare sugar allitol in its plant body. It is preferable that the body or the extract of the plant is contained in the beverage or food in the form of being packaged or filled in a unit of one food intake. Here, "a beverage or food in a form packed or filled in a unit of one food intake" means that the amount of beverage or food to be ingested at one time is packaged in a container such as a bag, a box or a bottle. Or, it means filled.

Furthermore, the food of the present invention may be used alone, but may be used in combination with other pharmaceutical composition, food composition or feed having an antiobesity effect or a blood triglyceride lowering effect. When used in combination, the anti-obesity effect such as the effect of suppressing the increase in body weight and the effect of suppressing the accumulation of body fat, the effect of slimming or the prevention and improvement of hypertriglyceridemia can be further enhanced.

The oral composition of the present invention may be provided with a label regarding the function. There is no particular limitation on the method of displaying the function, but examples include display on food packaging, container surfaces, food instructions, and food advertisements. Examples of the function of the oral composition of the present invention include a body fat accumulation suppressing function and/or a total cholesterol level increase suppressing function. The above-mentioned food has a concept of a body fat accumulation suppressing function and/or a total cholesterol level increase suppressing function, etc., and displays the fact as necessary, food for sick people, nutritionally functional food, health food or specified health care. Functional foods such as foods for use are included. Here, the function indications attached to these foods can be any of the main body of the product, the container, the packaging, the manual, the package insert, or the advertisement. The form of the food product may be solid, semi-solid or liquid.

The use as a food, a pharmaceutical product, a food additive, etc. may be a use in a human or non-human animal or a specimen derived therefrom, and may be a therapeutic use or a non-therapeutic use. Here, "non-therapeutic" means a concept that does not include a medical act, that is, a concept that does not include a method of operating, treating, or diagnosing a human, more specifically, a doctor or a person who receives an instruction from a doctor It is a concept that does not include a method of performing surgery, treatment, or diagnosis.
The food of the present invention is ingested in the natural world in a very small amount, and contains a rare sugar D-allulose and a sugar alcohol rare sugar allitol produced in the plant body or a body fat of the plant extract. It may be within the range of an effective amount that suppresses accumulation or within the range of an effective amount that lowers the total cholesterol level. Producing only a very small amount in nature in the food of the present invention, the content of the plant containing the rare sugar D-allulose and the sugar alcohol rare sugar allitol produced in the plant is usually about the whole food. The amount is 0.1 to 20% by mass, preferably about 0.5 to 10% by mass, more preferably about 1 to 5% by mass. The content of the plant extract is about 10% of the plant itself. In the natural world, only a very small amount is produced, and the effect is not expressed even if the content of the plant or the plant extract containing and producing the rare sugar D-allulose and sugar alcohol rare sugar allitol in the plant is too small. ..
For example, when the food of the present invention is ingested by an adult for the purpose of suppressing weight gain, the rare sugar D-allulose and sugar alcohol rarely produced in the natural world, although it varies depending on the object to be ingested, ingestion form, food intake, etc. The ingestion amount of a plant body that contains and contains sugar allitol in the plant body is generally 1 to 400 mg, preferably 40 to 400 mg as a dry weight per 1 kg of body weight per day. The above-mentioned amount may be ingested once, but may be ingested in several times. Producing only a very small amount in nature, the plant that contains and produces the rare sugar D-allulose and sugar alcohol rare sugar allitol in the plant, or the dose of the plant extract is not effective. .. Further, each of the above intake amounts is preferable from the viewpoint that the effect is exhibited without affecting the palatability or the intake amount. Similar amounts can be ingested for other animals.
Since the food thus obtained is safe, it can be continuously fed to vertebrates, preferably mammals, and particularly preferably humans.

A plant or an extract of the plant which contains and produces the rare sugar D-allulose and the sugar alcohol rare sugar allitol, which is produced only in a very small amount in nature in the above-mentioned pharmaceuticals (including quasi drugs). The content is usually 0.1% by mass or more, preferably 1.0% by mass or more, and 95% by mass or less, preferably 80% by mass or less, more preferably 60% by mass or less based on the total mass of the preparation. ..
The content of the plant or the plant extract containing the rare sugar D-allulose and the sugar alcohol rare sugar allitol produced and contained in the plant, which is produced only in a very small amount in the natural environment in the above foods, is the use thereof. The amount is usually 0.01% by mass or more and 50% by mass or less, preferably 20% by mass or less, and more preferably 10% by mass or less, though it depends on the form.
In the natural world of the present invention, which produces only a very small amount, a plant or a plant extract containing and producing the rare sugar D-allulose and the sugar alcohol rare sugar allitol in the plant, as a medicine or supplement, or a medicine or When used in combination with supplements, the dose is dependent on the form of the plant that produces and contains the rare sugar D-allulose and the sugar alcohol rare sugar allitol, which produces only a very small amount in nature, and , Rare sugar D, which may vary according to the condition of the subject, weight, sex, age or other factors, but the daily dose per adult when orally administered is usually very small in nature. -Allulose and sugar-alcohol rare sugar allitol are 15 mg or more, and 10 g or less, preferably 5 g or less, more preferably 1 g or less, as a plant or an extract of the plant that contains and contains allitol.
The above-mentioned preparation may be administered according to any administration schedule, but it is preferable to administer the preparation once a day to several times a day, continuously for several weeks to several months.
Moreover, the subject to be administered or ingested is not particularly limited as long as it is an animal in need or desire, but it is a human in need or desire of energy consumption promotion, suppression of body fat accumulation, or obesity prevention or improvement. Is mentioned.

[Example 1] Example 1 and Example 2 will be described below.

First, a dry powder of zuina was produced from zuina (young seedling). The results of nutritional component analysis of Zuina dry powder are shown in Table 4. As shown in Table 4, the dry powder of Zuina used in Example 1 contained about 10 g of allitol and 8 to 9 g of D-allulose (indicated as D-psicose in the figure) per 100 g of dry powder of Zuina, The content of rare sugar corresponded to about 5% of the dry powder of Zuina.

In Example 1, 16 3-week-old Wistar male rats were preliminarily bred, and then were bred for 8 weeks by being divided into 8 control diet groups and 8 zuina diet groups, respectively. The diet composition of each group is as shown in Table 5.

-Each diet is a high-fat and high-sucrose diet, and contains the same amount of protein, sugar and lipid. In the zuina food, the zuina dry powder is 5% of the total weight of the diet, that is, 50 g of the zuina dry powder is contained with respect to the total diet weight of 1000 g.

Then, the rats in each diet group were bred for 8 weeks under the following breeding conditions. Specifically, in the light-dark cycle, 8:00 to 20:00 is a light period, and 20:00 to 8:00 is a dark period. The temperature was 22±1° C., and food and water were freely available. And body weight and food intake were recorded daily. After the rearing period, the rats in each diet group were sacrificed without fasting.

After the rats in each diet group were bred for 8 weeks, they were sacrificed and dissected, and serum components and tissue components were collected as shown in FIG. Then, the inspection shown in FIG. 1 was performed using each of the collected tissues. Note that FIG. 1 is a diagram for explaining a collected tissue and an inspection item in the first embodiment.

FIG. 2 is a graph showing a change in average body weight of the rats of each diet group after 8 weeks of breeding in Example 1. As shown in FIG. 2, the weight of the rats of the Zuina diet group tended to be lower than that of the rats of the control diet group, but this was not a significant difference.

Also, FIG. 3 is a graph showing the final body weight, weight gain, dietary intake and dietary efficiency of the rats in each diet group. Regarding the final body weight, the amount of weight gain, the food intake, and the food efficiency, the rats of the Zuiina diet group tended to have a lower body weight than the rats of the control diet group, but they were not significantly different.

FIG. 4 is a graph showing the relative tissue weights of the heart, liver, spleen, and kidney of the rats in each diet group when the weight of the rats is 100 g. As shown in FIG. 4, regarding the heart, spleen, and kidney, there was no significant difference between the rats in the diet diet group and the rats in the control diet group, but in the liver, the rats in the diet diet group were compared with the control diet group. The weight was significantly lower than that of rats.

FIG. 5 is a graph showing the relative tissue weights of perirenal adipose tissue, epididymal adipose tissue, mesenteric adipose tissue, and total abdominal adipose tissue of rats in each diet group when the weight of the rat is 100 g. .. As shown in FIG. 5, with respect to perirenal adipose tissue, epididymal adipose tissue, mesenteric adipose tissue, and total abdominal adipose tissue, the weight of the rats of the Zuina diet group was lower than that of the rats of the control diet group. There was a tendency, but it was not a significant difference.

FIG. 6 is a graph showing total protein, lipase, total cholesterol, and chlor among the serum components of rats in each diet group. As shown in FIG. 6, the total protein, lipase, and total cholesterol of the rats of the Zuiina diet group were significantly lower than those of the rats of the control diet group. On the other hand, regarding crawl, the rats of the Zuiina diet group showed a significantly higher value than the rats of the control diet group. No significant difference was observed except for the serum components listed in FIG.

FIG. 7 is a graph showing glycogen, total fat, cholesterol, and neutral fat in the liver components of rats in each diet group. As shown in FIG. 7, the rats in the diet diet group tended to have lower amounts of glycogen, total fat, cholesterol, and triglyceride in the liver as compared with the rats in the control diet group, but to a significant difference. There wasn't. It is considered that one of the factors that the liver weight of the rats of the Zuina diet group was lower than that of the rats of the control diet group was that fat accumulation was reduced.

FIG. 8 is a graph showing carcass fat mass, carcass fat mass, total body fat mass, and total body fat mass of rats in each diet group. As shown in FIG. 8, the carcass fat amount, carcass fat percentage, total body fat amount, and total body fat percentage were significantly lower in rats of the zuina diet group than in rats of the control diet group. ..

(About toxicity of Zuina dry powder)
As shown in FIG. 6, the total protein of the rats of the Zuiina diet group showed a significantly lower value than the rats of the control diet group. Although total protein is a standard for knowing the condition of the liver and kidneys, among serum components not shown, ALS and AST, which are indicators of liver function, urea nitrogen, creatinine, and serum protein abnormalities, which are indicators of renal function. There is no significant difference in the A/G ratio, which is an index of the above, between the rats of the Zuiina diet group and the rats of the control diet group, and it is considered to be within the range of changes that can normally occur.
In addition, as shown in FIG. 6, the total cholesterol level of the rats in the Zuiina diet group was significantly lower than that of the rats in the control diet group. Total cholesterol was an indicator of abnormal lipid metabolism, but there was no significant difference in HLD cholesterol, non-HDL cholesterol, and neutral fat (not shown). In addition, as shown in FIG. 7, the cholesterol level in the liver also tends to be lower in rats in the diet diet group than in the control diet group. It was suggested that the significant decrease compared with the rats in the control diet group was due to the components in zuina.
As shown in FIG. 6, for lipase, the rats in the zuiner diet group showed a significantly lower value than the rats in the control diet group, and for crawl, the rats in the zuiner diet group had a significantly lower value. The value was significantly higher than that of the rat. Lipase is an indicator of pancreatic disease, and chlor is involved in the adjustment of pH and osmotic pressure, but since both are components that are affected by diet, blood should be collected on an empty stomach. It is considered that there was a significant difference because the animals were sacrificed without fasting.
As shown in FIG. 6, among the serum components, there was a significant difference in the total protein, lipase, total cholesterol, and chlor between rats in the Zuina diet group and rats in the control diet group. I knew it wasn't.

(Zuina dry powder suppresses body fat accumulation)
As shown in FIG. 8, the carcass fat and the total body fat of the rats of the Zuiina diet group were significantly lower than those of the rats of the control diet group. From this, it is suggested that Zuina dry powder has a body fat accumulation suppressing function.

(Zuina dry powder suppresses the rise in total cholesterol)
As shown in FIG. 6, in the serum components, the rats of the Zuiina diet group showed significantly lower levels of total cholesterol than the rats of the control diet group. Further, as shown in FIG. 7, the cholesterol in the liver tissue of the rats of the Zuiina diet group tended to be lower than that of the rats of the control diet group. From this, it is suggested that Zuina dry powder has a function of suppressing the increase in total cholesterol level.

Thus, it was found that Zuina dry powder has no toxicity and has a function of suppressing body fat accumulation and a function of suppressing increase in total cholesterol level. Such a function is considered to be due to the action of the rare sugars D-allulose and allitol contained in Zuina. Therefore, the following Example 2 was carried out in order to examine whether or not there is a difference in function between the dried powder of Zuina and D-allulose and allitol extracted from natural products.

First, a dry powder of zuina was produced from zuina (young seedling). Table 6 shows the results of nutritional component analysis of Zuina dry powder. As shown in Table 6, the dry powder of Zuina used in Example 2 contained 12.1 g of allitol and 7.8 g of D-allulose (denoted as D-psicose in the figure) per 100 g of dry powder of Zuina. , About 5% of the dry powder of Zuina.

In Example 2, after 32 week-old male Wistar rats of 3 weeks were preliminarily bred, the control diet group was 8 animals, the zuina diet group was 8 animals, the rare sugar diet group was 8 animals, and the green tea diet group was 8 animals, respectively. The animals were divided and kept for 8 weeks. The diet composition of each group is as shown in Table 7.

-Each diet is a high-fat and high-sucrose diet, and contains the same amount of protein, sugar and lipid. Further, in the zuina diet, the zuina dry powder is 5% of the total weight of the diet, that is, 50 g of the zuina dry powder is contained with respect to the total diet weight of 1000 g. Similarly, in the green tea food, the dry powder of green tea is 5% of the total weight of the diet, that is, 50 g of the dry powder of green tea is contained for 1000 g of the total diet weight. In addition, since 50 g of zuina powder contained in zuina food contains 3.9 g of D-allulose (indicated as D-psicose in the figure) and 6.1 g of allitol, it is extracted from natural products in rare sugar diet D-allulose and allitol were mixed in the same amount as the Zuna diet, that is, 3.9 g of D-allulose and 6.1 g of allitol were mixed.

Then, the rats in each diet group were bred for 8 weeks under the following breeding conditions. Specifically, in the light-dark cycle, 8:00 to 20:00 is a light period, and 20:00 to 8:00 is a dark period. The temperature was around 25°C, the humidity was around 68%, and food and water were freely available. And body weight and food intake were recorded daily. After the rearing period, the rats in each diet group were sacrificed without fasting.

After the rats in each diet group were bred for 8 weeks, they were sacrificed and dissected, and each tissue component of the rats was collected as shown in FIG. Then, the inspection items shown in FIG. 9 were inspected using each of the collected tissues. Note that FIG. 9 is a diagram for explaining the collected tissue and the inspection item in the second embodiment.

FIG. 10 is a graph showing changes in body weight of rats in each diet group, final body weight, dietary intake, and dietary efficiency. In the following description, rats in the control diet group will be described as group C, rats in the zuiina diet group as IT group, rats in the rare sugar diet group as RS group, and rats in the green tea diet group as G group. Regarding the body weight transition and the final body weight, the weight of the rats in the green tea diet group (G group) tended to be low, and it was significant only between the rats in the zuina diet group (IT group) and the rats in the green tea diet group (G group). A difference was observed. Regarding the food intake, no significant difference was observed between the rats of each diet group, but the dietary efficiency of the rats of the green tea diet group (G group) was significantly lower than that of the rats of other diet groups.

FIG. 11 is a graph showing the relative tissue weights of the heart, liver, kidney, and spleen of the rats in each diet group when the weight of the rats is 100 g. As shown in FIG. 11, regarding the heart and kidney, there was no significant difference between the rats of each diet group, but regarding the liver and spleen, the rats of the green tea diet group (G group) were the rats of other diet groups. It was a low value compared to. Specifically, in the liver, the rats in the green tea diet group (group G) were significantly lower than those in the duina diet group (IT group), and in the spleen, the zina diet group and the rare sugar group. Rats (G group) in the green tea diet group were significantly lower than those of the rats (IT group, RS group).

FIG. 12 is a graph showing relative tissue weights of epididymal adipose tissue, perirenal adipose tissue, mesenteric adipose tissue, and total abdominal adipose tissue of rats in each diet group when the weight of the rat is 100 g. .. As shown in FIG. 12, in all of epididymal adipose tissue, perirenal adipose tissue, mesenteric adipose tissue, and total abdominal adipose tissue, the rats in the green tea diet group (G group) were compared with the control diet group and the zina diet group. The value was significantly lower than that of the rats in the rare sugar diet group (C group, IT group, RS group). Regarding the perirenal adipose tissue and the total abdominal adipose tissue, the rats of the Zuiina diet group (IT group) showed significantly lower values than the rats of the control diet group (C group).

FIG. 13 is a graph showing carcass fat mass, carcass fat mass, total body fat mass, and total body fat mass of rats in each diet group. As shown in FIG. 13, regarding the total body fat amount, the rats of the green tea diet group (G group) were compared with the rats of the control diet group, the Zuna diet group and the rare sugar diet group (C group, IT group, RS group). Showed a significantly lower value. Regarding the carcass fat percentage, rats in the green tea diet group (G group) showed significantly lower values than those in the control diet group and the rare sugar diet group (C group, IT group, RS group). It was Furthermore, regarding the total body fat percentage, rats of the Zuiina diet group (IT group) showed a significantly lower value than rats of the control diet group (C group), and rats of the green tea diet group (G group) further showed It showed a low value.

FIG. 14 is a graph showing the test results of the concentrations of glucose, insulin and neutral fat in the serum components of the rats in each diet group. As shown in FIG. 14, there was no significant difference in the concentration of glucose among the serum components between the rats in each diet group, but the insulin concentration and the triglyceride concentration were not significantly different in the rats in the green tea diet group (G group). ) Was significantly lower than the rats in the control diet group, the Zuiina diet group, and the rare sugar diet group (C group, IT group, RS group).

FIG. 15 is a graph showing the test results for the concentrations of total cholesterol, HDL cholesterol, and free fatty acids in the serum components of rats in each diet group. As shown in FIG. 15, among the serum components, the concentrations of total cholesterol and free fatty acids were significantly lower in the rats of the green tea diet group (G group) than in the rats of the control diet group (C group). The value was shown. Regarding the concentration of HDL cholesterol, the rats of the Zuiina diet group and the rare sugar diet group (IT group, RS group) showed a significantly lower value than the rats of the control diet group (C group), and the green tea diet group Rats (Group G) showed even lower values.

(Zuina dry powder anti-obesity function)
As shown in FIG. 12 and FIG. 13, regarding the perirenal adipose tissue, the total abdominal adipose tissue, and the total body fat percentage, the rats of the zuina diet group (IT group) were compared with the rats of the control diet group (C group). It was a significantly low value. From this, also in Example 2, it was suggested that Zuina dry powder has a body fat accumulation suppressing function. In addition, as shown in FIG. 15, HDL cholesterol and free fatty acids in the serum components were significantly lower in rats of the Zuiina diet group (IT group) than in rats of the control diet group (C group). became.
In rats (RS group) in the rare sugar diet group, epididymal adipose tissue, perirenal adipose tissue, and mesenteric adipose tissue and total abdominal adipose tissue adipose tissue, insulin, neutral fat, free fatty acid, total fatty acid, With regard to serum components of cholesterol and HDL cholesterol, and carcass fat amount, carcass fat percentage, total body fat percentage and total body fat percentage, body fat of the body showed the same tendency as that of the Zuna diet group rat (IT group), and a control diet. The value was relatively low as compared to the group of rats (C group).
Therefore, it is suggested that zuina dry powder has an antiobesity function under the condition of high-fat and high-sucrose diet, like the rare sugar extracted from natural products.

In particular, since the test results tended to be the same between rats of the Zuina diet group (IT group) and rats of the rare sugar diet group (RS group), such a function was found in the rare sugar D-allulose and It is considered to be the action of allitol). In Example 2, the anti-obesity function was suggested even though the dietary addition amount of D-allulose was only 0.4%. Therefore, not only the action of D-allulose but also allitol (co-administered simultaneously) It is considered to be due to the effect of (0.6% content) or the synergistic effect of D-allulose and allitol.

(Combination with green tea food)
Further, in Example 2, the rats in the green tea diet group (Group G) showed very low values in terms of body weight, diet efficiency, total intraperitoneal fat, serum components, and body fat, compared with other diet groups. It is considered that the anti-obesity effect possessed has acted. Therefore, it is expected that the dry powder of Zuina is mixed with a dietary fiber-containing material such as green tea and taken to more efficiently exhibit an antiobesity effect.

According to the present invention, the plant itself containing and producing the rare sugar D-allulose and the sugar alcohol rare sugar allitol, which are produced only in a very small amount in nature, can be easily ingested as an oral preparation, and body fat Since the effects of suppressing the accumulation, suppressing the increase in the total cholesterol value, and the like can be expected, the industrial utility of the present invention is high.

Claims

Producing only a very small amount in nature, characterized by containing a rare sugar D-allulose and a sugar alcohol rare sugar allitol produced in the plant and containing the plant or the plant extract as an active ingredient, Body fat accumulation suppressor or total cholesterol level increase suppressor.
The body fat accumulation suppressor or the total cholesterol level increase suppressor according to claim 1, wherein the plant is Zuina or Ryobu.
The body fat accumulation suppressor or the total cholesterol level increase suppressor according to claim 1 or 2, wherein the plant is a plant that has been treated to increase the content of rare sugars.
The plant is a mature tree, a mature tree that has been subjected to D-allulose increasing treatment, and/or buds, leaves, branches, flowers or roots of mutants thereof, or germinated seeds, subculture or tissue culture. The body fat accumulation suppressor or the total cholesterol level increase suppressor according to any one of claims 1 to 3, which is the whole grown plant.
The body fat accumulation suppressor or the total cholesterol level increase suppressor according to any one of claims 1 to 4, wherein the plant is a dried product.
The body fat accumulation suppressor or the total cholesterol level increase suppressor according to claim 5, wherein the dried product is a freeze-dried product.
The body fat accumulation inhibitor or the total cholesterol level elevation inhibitor according to claim 6, wherein the freeze-dried product is a freeze-dried product of a freeze-dried body immersed in liquid nitrogen by a freeze-drying device.
The body fat accumulation inhibitor or total cholesterol level elevation inhibitor according to claim 6 or 7, wherein the freeze-dried product is a pulverized product or a crushed product of the freeze-dried product.
The body fat accumulation suppressor or the total cholesterol level increase suppressor according to claim 8, wherein the crushed product or crushed product is a powdery product.
5. The body fat accumulation inhibitor according to any one of claims 1 to 4, or the total cholesterol level increase inhibition, wherein the plant extract is a water extract or an alcohol extract of an aqueous medium extract residue of the plant. Agent.
The body fat accumulation suppressor or the total cholesterol level increase suppressor according to any one of claims 1 to 10, wherein the plant or the plant extract is in the form of powder, paste or solution.
The body fat accumulation suppressor or the total cholesterol level increase suppressor according to any one of claims 1 to 11, which is orally administered.
Producing only a very small amount in the natural world, a plant containing and producing the rare sugar D-allulose and the sugar alcohol rare sugar allitol in the plant, and/or containing the plant extract, An oral composition having a body fat accumulation suppressing function and/or a total cholesterol level increase suppressing function.
The oral composition according to claim 13, wherein the plant is Zuina or Ryobu.
The oral composition according to claim 13 or 14, wherein the plant is a plant that has been treated to increase the content of rare sugars.
The plant is a mature tree, a mature tree that has been subjected to D-allulose increasing treatment, and/or buds, leaves, branches, flowers or roots of mutants thereof, or germinated seeds, subculture or tissue culture. The oral composition according to any one of claims 13 to 15, which is a whole grown plant.
The oral composition according to any one of claims 13 to 16, wherein the plant is a dried product.
The oral composition according to claim 17, wherein the dried product is a freeze-dried product.
The oral composition according to claim 18, wherein the freeze-dried product is a freeze-dried product obtained by freeze-drying a liquid nitrogen-immersed frozen body.
The oral composition according to claim 18 or 19, wherein the freeze-dried product is a pulverized product or a crushed product of the freeze-dried product.
The oral composition according to claim 20, wherein the crushed product or crushed product is a powdery product.
The oral composition according to any one of claims 13 to 16, wherein the plant extract is a water extract or alcohol extract of the aqueous medium extract residue of the plant.
The oral composition according to any one of claims 13 to 16, wherein the plant or the plant extract is in the form of powder, paste or solution.
The oral composition according to any one of claims 13 to 23, further comprising a dietary fiber-containing material.
The oral composition according to claim 24, wherein the dietary fiber-containing material is tea powder selected from Japanese tea, black tea and Chinese tea.
The oral composition according to claim 24, wherein the dietary fiber-containing material is green plant powder.
The oral composition according to claim 24, wherein the dietary fiber-containing material comprises one or more of ashitaba, barley leaf, green tea, kale, sweet potato leaf, broccoli, moloheiya, sesame seed leaf, mulberry leaf, peony, and mugwort.
The oral composition according to any one of claims 13 to 27, which is a food product, a quasi drug, or a pharmaceutical product.
The oral composition according to claim 28, which is used as a health food for suppressing body fat accumulation, a health drink or a medicine.
29. The oral composition according to claim 28, which is used as a health food, a health drink or a medicine for suppressing an increase in total cholesterol level.
29. The food according to claim 28, wherein the food is prepared as a supplement, a functional food, a health food, an enteral nutritional food, a special-purpose food, a dietary supplement, a food for specified health use, or a nutritionally functional food. The oral composition according to any of 30.
The oral composition according to claim 31, wherein the special-purpose food is a food for the sick.
The oral composition according to any one of claims 13 to 32, which is prepared in the form of a tablet, a round, a capsule, a powder, a granule, a fine granule, a troche, or a liquid. ..
The oral composition according to claim 33, which is a granule or a tablet.
The food is prepared as a beverage, a confectionery, a frozen dessert, a noodle, a processed seafood, a processed livestock food, a dairy product, a fat and oil and a processed fat, a seasoning, an enteral nutritional food, a soup, a stew, a side dish, a pickle, or a bread. The oral composition according to any one of claims 28 to 34, which is characterized in that