WO2020155385A1 - Procédé de distribution de liquide et procédé de dosage immunologique - Google Patents

Procédé de distribution de liquide et procédé de dosage immunologique Download PDF

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Publication number
WO2020155385A1
WO2020155385A1 PCT/CN2019/082166 CN2019082166W WO2020155385A1 WO 2020155385 A1 WO2020155385 A1 WO 2020155385A1 CN 2019082166 W CN2019082166 W CN 2019082166W WO 2020155385 A1 WO2020155385 A1 WO 2020155385A1
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WIPO (PCT)
Prior art keywords
reactor
sample
station
unit
ferry
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PCT/CN2019/082166
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English (en)
Chinese (zh)
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张震
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深圳迎凯生物科技有限公司
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Publication of WO2020155385A1 publication Critical patent/WO2020155385A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers

Definitions

  • This application relates to the field of in vitro diagnostic technology, and in particular to a liquid dispensing method and an immunoassay method including steps in the liquid dispensing method.
  • the automatic immunoassay analyzer can quantitatively or qualitatively detect the target analytes such as antibodies and antigens contained in the blood samples waiting to be tested.
  • the test samples and reagents or reactants
  • the test samples and reagents are distributed in the empty reactor and mixed After homogenization, incubation and washing separation (Bound-free, combined separation, ie BF separation, etc.), signal reagents are distributed in the reactor to measure optical or electrical signals, thereby realizing the measurement of the target analyte contained in the sample to be tested analysis.
  • test flux can be understood as the number of test results that the immunoassay analyzer can report in a unit of time, that is, the number of reactors that contain the target analyte. The greater the total number of reactors measured in a time, the higher the test throughput of the immune analyzer. Because the reaction mode and test process of the analysis project are usually different, the test throughput of the immune analyzer is not static. The maximum test throughput is usually used as a measure of the speed of the immune analyzer. This application is for convenience of description, unless otherwise specified, the test Throughput refers specifically to the maximum test throughput of the analyzer. Consider the processing of the reactor by the immune analyzer as a pipeline.
  • N reactors containing the target analyte in a unit of time to complete the measurement and leave the pipeline in order to ensure that the test is continuously and reliably performed at the maximum throughput, it must be performed at the same time
  • N empty reactors entering the pipeline that is, the flow rate of the reactor at the inlet of the pipeline (inlet flow rate) is equal to the flow rate at the outlet (outlet flow rate).
  • the flow of each link in the middle of the pipeline of the reactor should be equal to the inlet flow and the outlet flow, that is, the flow rates in all parts of the pipeline are equal.
  • a technical problem solved by this application is how to improve the working efficiency of liquid distribution.
  • a liquid distribution method including:
  • the sample can be distributed to the empty reactor on the buffer unit at the same time.
  • An immunoassay method includes the steps in the liquid dispensing method described above.
  • Fig. 1 is a schematic diagram of a plane structure of a first example immune analyzer provided by the first embodiment.
  • Fig. 2 is a partial three-dimensional structure diagram of the ferry unit in Fig. 1.
  • Fig. 3 is a schematic plan view of a second example immune analyzer provided by the first embodiment.
  • Fig. 4 is a schematic plan view of a third example immune analyzer provided by the first embodiment.
  • Fig. 5 is a schematic diagram of the planar structure of the immune analyzer provided in the second embodiment.
  • Figure 6 is a schematic diagram of continuously distributing the same sample to at least two reactors.
  • Figure 7 is a timing diagram of the distribution of samples and reagents to the reactor.
  • Figure 8 is a schematic diagram of providing diluted samples to at least two reactors.
  • Figure 9 is a flow chart of the first liquid dispensing method.
  • Figure 10 is a flow diagram of the second liquid dispensing method.
  • Figure 11 is a flow chart of the sample dilution method.
  • Figure 12 is a flow chart of the third liquid dispensing method.
  • Figure 13 is a timing diagram of the third liquid dispensing method for dispensing samples and reagents to the reactor.
  • the incubation of the sample and reagent specifically refers to the antigen-antibody binding reaction or biotin avidin binding of the reactants in the reactor 20 before the start of cleaning and separation.
  • the reaction process The reagents and analysis items described here have a "one-to-one correspondence" relationship, that is, the specific reagents corresponding to different analysis items are generally different in terms of formula, reagent quantity, component quantity, etc.
  • reagents usually include multiple components, such as common 2-5 components, including magnetic particles, markers, diluents, dissociating agents, and other reagent components (the corresponding reagent components can be separated Represented by R1, R2, R3, R4).
  • T4 reagent thyroxine
  • R1 magnetic particles
  • R2 labeling
  • R4 reagent dissociating agent
  • multiple reagent components of an analysis item can be distributed at one time or in multiple steps. When distributed in steps, it is defined as first reagent, second reagent, third reagent, etc. according to the order of distribution. After the incubation is completed, cleaning and separation are carried out.
  • Cleaning and separation refers to the use of a magnetic field to capture the bound magnetic particles and labeled complexes, while removing free (free) markers and other unreacted or bound components (this text is convenient for expression, referred to as Unbound components) process.
  • the signal reagent is distributed, the signal is incubated (generally 1-6 minutes), and finally the amount of luminescence produced by the reaction between the labeling reagent and the signal reagent is measured (this text is convenient for expression, called reactant signal).
  • the signal reagent is used to measure the signal (usually the amount of luminescence), usually a kind of general reagent, and the corresponding relationship with the analysis item is "one-to-many", that is, different analysis items share the signal reagent.
  • Signal incubation refers to a process in which the cleaned and separated reactor 20 is reacted for a period of time in a constant temperature environment after the signal reagent is distributed to increase the signal. It should be pointed out that due to the difference in the specific components of the signal reagent, some luminescence systems do not require signal incubation, and can be directly measured during or after the signal reagent is distributed.
  • the signal reagent can be one or more, for example, some signal reagents include a first signal reagent, a second signal reagent, and so on.
  • the immune analyzer 10 can perform analysis corresponding to several different analysis items on the sample.
  • Work cycle or cycle is the shortest time window that can be reproduced cyclically during the test. It usually has a fixed length of time. Within the cycle time, a certain number of process operations, tasks or work packages, such as liquid withdrawal Operations and tasks such as mixing, incubating, washing and separating, and measuring are performed serially or in parallel in a controlled sequence. The tasks of the same component in a cycle are usually executed in series, and the tasks of different components in the same cycle depend on whether there is a dependency between the actions of related components and can be executed serially or in parallel. All process operations performed in one cycle are executed only when needed, and not necessarily repeated in another cycle. In particular, certain process operations may repeat in every cycle, while others may occur every two or more cycles.
  • each test is usually at a different stage of the test process.
  • process operations that occur in a single cycle only certain process operations are dedicated to executing a test, and other process operations are used for Perform other tests.
  • the time length of multiple parallel cycles has a multiple relationship, and the multiple is usually equal to the number of the same component.
  • each ferry unit 200 works in the first cycle.
  • the cycle length is N times the second cycle, and the action sequences of the N ferry units 200 are continuously "staggered in parallel" for the second cycle.
  • the immune analyzer 10 provided by an embodiment of the present application includes a liquid distribution device 11 and a reaction device 12.
  • the liquid distribution device 11 is located beside the reaction device 12, and the liquid distribution device 11 is used in the reactor 20. Complete the distribution of samples and reagents, and the mixing of samples and reagents.
  • the reaction device 12 is used for incubating, cleaning, separating, and measuring the sample and reagent (reactant) after mixing in the reactor 20.
  • the liquid distribution device 11 includes a buffer unit 100, a supply silo 130, a supply slide 140, a ferry unit 200, a sample addition part 300, a sample delivery unit 400, a reagent distribution part 500, a storage unit 600, a transfer unit 700, a cleaning tank 800 and Sorting agency.
  • the liquid distribution device 11 may also include a sample adding drive unit 301, a reagent drive unit 501, and a sample adding power unit and a reagent distribution drive.
  • the sample adding piece 300 is installed on the sample adding drive unit 301, and the sample adding drive unit 301 is used for The sample adding member 300 is driven to move so that the sample adding member 300 can absorb or remove the sample under the action of the sample adding power device.
  • the reagent distributing member 500 is installed on the reagent driving unit 501, and the reagent driving unit 501 is used to drive the reagent distributing member 500 to move so that the reagent distributing member 500 sucks or discharges the reagent under the reagent distributing power unit.
  • the sample loading power unit and the reagent distribution power unit can use general fluid quantitative devices such as syringes, plunger pumps, and quantitative pumps.
  • the sample conveying unit 400 may include a sample rack 410, a sample tube 430, and a conveying rail 420.
  • the sample rack 410 may be matched with the conveying rail 420.
  • the sample tube 430 is placed on the sample rack 410.
  • the sample tube 430 is used to hold samples. Five to ten sample tubes 430 can be placed on one sample rack 410.
  • the sample rack 410 drives the sample tube 430 to move to a designated position along the conveying track 420, the sample adding component 300 aspirates a sample of the tube 430 and distributes the sample to the empty reactor 20.
  • the supply silo 130 is used to store clean and empty reactors 20, and the sorting mechanism can be used to sort the scattered reactors 20 from the supply silo 130 to arrange them in a certain order, and the supply chute 140 will sort the reactions.
  • the reactors 20 are introduced into the buffer unit 100 one by one, and the buffer unit 100 is used to buffer the reactor 20 conveyed by the supply chute 140.
  • the entire liquid distribution device 11 has a receiving station 33, a sample adding station 34 and a removing station 35.
  • the buffer unit 100 includes a turntable 110, which can rotate around its own central axis. A plurality of buffer positions 101 are provided on the turntable 110. The buffer positions 101 are used to carry the reactor 20.
  • the buffer positions 101 can be accommodating holes. The accommodating hole can also be replaced by a solid structure such as a bracket, as long as the reactor 20 can be placed on the turntable 110.
  • the buffer positions 101 are distributed along the circumferential interval of the turntable 110.
  • the buffer positions 101 can be driven to move between the receiving station 33, the sample loading station 34 and the removal station 35, so that the reaction on the turntable 110
  • the device 20 moves between the receiving station 33, the sample loading station 34, and the removing station 35.
  • the turntable 110 drives the reactor 20 to make a circular movement between the receiving station 33, the sample loading station 34, and the removing station 35.
  • the reactor 20 from the supply chute 140 will enter the buffer position 101 on the turntable 110 at the receiving station 33.
  • the sample 300 can aspirate the sample from the sample tube 430 to distribute it to the reactor 20.
  • the transfer unit 700 will have the sample The reactor 20 is separated from the turntable 110 at the removal station 35 and transferred to the ferry unit 200.
  • the buffer bits 101 are arranged at intervals along the circumference of the turntable 110, and the buffer units 100 may only be arranged to form a buffer circle, which is arranged close to the edge of the turntable 110.
  • the cache bits 101 can also be arranged to form multiple cache circles, and the multiple cache circles are arranged concentrically around the center axis of the turntable 110.
  • the cache unit 100 includes a slider 120.
  • the slider 120 is also provided with a cache position 101 for placing the reactor 20, and the cache position 101 on the slider 120 may also be a receiving hole.
  • the cache bits 101 may only be distributed along a straight line on the slider 120 to form a row.
  • the cache bits 101 may be distributed on the slider 120 along a straight line to form multiple rows.
  • the multiple rows of cache bits 101 are arranged on the slider 120 in a matrix distribution.
  • the sliding block 120 moves linearly between the receiving station 33, the sample loading station 34 and the removal station 35, thereby driving the buffer position 101 (corresponding to the reactor 20) on it in the receiving station 33, sample loading station 34 and Move out of the station 35 to move.
  • the reactor 20 enters the sliding block 120 at the receiving station 33, the sample addition member 300 distributes the sample to the reactor 20 located at the sample addition station 34, and the transfer unit 700 will be filled
  • the reactor 20 with the sample is separated from the slider 120 at the removal station 35 and transferred to the ferry unit 200.
  • the slider 120 and the buffer position 101 on it linearly reciprocate between the receiving station 33, the sample adding station 34, and the removing station 35 to complete the receiving, sample distribution, and full storage of the reactor 20
  • the slider 120 itself can be designed to be smaller in volume.
  • the area covered by the linear motion track of the slider 120 is smaller, which is beneficial to the supply bin 130, the supply slide 140, and the sample.
  • the optimized spatial layout of the conveying unit 400, the sample addition part 300, the transfer unit 700, etc. can make the liquid distribution device 11 more compact and lower in cost.
  • the cache unit 100 includes both a turntable 110 and a slider 120 each provided with a cache bit 101.
  • the turntable 110 rotates around its own central axis, and the turntable 110 can drive the cache bit 101 on it to pass
  • the receiving station 33 and the reactor 20 on the supply chute 140 will enter the buffering position 101 on the turntable 110 at the receiving station 33.
  • the slider 120 moves linearly between the sample loading station 34 and the removal station 35.
  • the transfer unit 700 can separate the empty reactor 20 from the turntable. 110 and transferred to the sliding block 120 at the removal station 35.
  • the sample loading part 300 distributes the sample to the empty reactor 20, and then the sliding block 120 After driving the reactor 20 containing the sample to the removal station 35, the transfer unit 700 separates the reactor 20 containing the sample from the removal station 35 from the slider 120 and transfers it to the ferry unit 200.
  • the liquid distribution device 11 further includes a frame 210, a conveyor 220, a support 230, and a driver 240.
  • the conveyor 220 is arranged on the frame 210, and the conveyor 220 is used to drive the support 230.
  • the conveyor 220 includes a motor 221, a driving wheel 222, a driven wheel 223, and a timing belt 224.
  • the motor 221 is used to drive the driving wheel 222 to rotate.
  • the timing belt 224 is wound around the driving wheel 222 and the driven wheel 223.
  • the transmitter 220 can also be replaced by one or more of transmission mechanisms such as a screw mechanism and a rack and pinion.
  • the rack 210 may be provided with a sliding rail 225, the support 230 is matched with the sliding rail 225, the timing belt 224 is connected with the support 230 and drives the support 230 to slide along the extending direction of the sliding rail 225, the driver 240 and the ferry unit 200
  • the ferry unit 200 is used to place the reactor 20, and the driver 240 can drive the ferry unit 200 to produce eccentric oscillations, so that the samples and reagents (reactants) in the reactor 20 produce non-contact eccentric oscillations. And to achieve mixing.
  • the ferry unit 200 may be provided with a plurality of receiving holes, and the reactor 20 is inserted into the receiving holes, so as to realize the bearing function of the ferry unit 200 to the reactor 20.
  • the receiving hole can also be replaced by a solid structure such as a bracket, as long as the reactor 20 can be placed on the ferry unit 200.
  • the entire liquid distribution device 11 also has an initial station 30 and a first station 31.
  • the ferry unit 200 can reciprocate between the initial station 30 and the first station 31. Linear motion.
  • the transfer unit 700 can transfer the reactor 20 containing samples from the buffer unit 100 to the ferry unit 200 at the initial station 30.
  • the ferry unit 200 drives the reactor 20 containing the sample to move to the first station 31, and the reagent distribution member 500 will suck reagents from the storage unit 600 for storing reagents and distribute the reagents to the first station. 31 of the reactor 20.
  • the driver 240 can drive the ferry unit 200 to eccentrically oscillate, thereby mixing the samples and reagents in the reactor 20, so that the mixing of reactants and the movement of the ferry unit 200 can be achieved.
  • the ferry unit 200 can simultaneously mix the reactants in the reactor 20 during the movement process, which improves the mixing efficiency and mixing effect, thereby increasing the test throughput of the whole machine.
  • the driver 240 can also drive the ferry unit 200 to eccentrically oscillate to mix the samples and reagents in the reactor 20 uniformly.
  • the ferry unit 200 returns the reactor 20 after reagent distribution to the initial station 30.
  • the transfer unit 700 can separate the reactor 20 with the reactants from the initial station 30 from the ferry unit 200 and transfer it to the reaction device 12 for reaction.
  • the reactor 20 performs incubation, washing and separation, and measurement processing on the reaction device 12.
  • the driver 240 directly oscillates the ferry unit 200 eccentrically, so that the reactants in the reactor 200 on the ferry unit 200 can be mixed uniformly. No additional independent mixing device is needed.
  • the ferry unit 200 is linearly moved or stationary. The mixing of the reactants in the reactor 200 is not restricted, and the problems of the complicated mixing device, low mixing efficiency and poor mixing effect of the prior art are solved.
  • the apron unit 200 performs linear motion between the sample loading part 300, the transfer unit 700, and the reagent distribution part 500. On the one hand, the control difficulty of linear motion is reduced, so that the apron unit 200 moves more accurately and efficiently, and prevents the apron unit 200 from deviating from the designated stop.
  • the reagent distributor 500 reliably adds the reagents to the reactor 20 at the first station 31; on the other hand, the reactor 20 containing the sample is carried on the buffer unit 100 to be transferred to the ferry unit 200 to add reagents and mix. It is not necessary to transfer all the reactors 20 containing samples to the ferry unit 200, which fully utilizes the carrying space of the buffer unit 100, reduces the number of reactors 20 carried by the ferry unit 200 at the same time, and ensures the ferry unit
  • the volume of 200 is designed to be smaller and the structure is more compact.
  • the area covered by the linear motion track of the ferry unit 200 is smaller, which solves the problem that the sample addition piece 300, the transfer unit 700, and the reagent distribution piece 500 in the prior art must be along
  • the large-radius rotation or the restriction of the rotary disk layout optimizes the spatial layout and control process between components or units, and can more efficiently connect and coordinate the logical actions among the sample addition unit 300, the transfer unit 700, and the reagent distribution unit 500.
  • the immune analyzer can be made more compact and the overall work efficiency can be improved.
  • the reactor 20 By using the reactor 20 on the buffer unit 100 for sample distribution, the reactor 20 after the sample distribution is transferred to the ferry unit 200 for reagent distribution and mixing, so that the sample distribution and the reagent distribution are in different independent units.
  • the realization and mixing are directly realized on the ferry unit, which solves the mutual limitation of the distribution of samples and reagents in the prior art and the problem of mixing the reactants individually at a specific station, and improves the efficiency of liquid distribution and mixing .
  • the storage unit 600 is a rotatable disk, and the storage unit 600 is arranged close to the first station 31.
  • a plurality of storage parts 610 are provided on the storage unit 600.
  • the storage part 610 is used to place and store reagent containers. It is placed in a reagent container, and the reagent distributing member 500 is used for sucking the reagent components in the reagent container on the storage part 610 and distributing the reagent components into the reactor 20 at the first station 31.
  • the number of storage units 610 can be set according to needs.
  • the number of storage units 610 on each storage unit 600 is preferably 15-50, for example, the storage units on each storage unit 600 The number of 610 is 25, so that two storage units 600 can store 50 reagent containers online at the same time.
  • Each storage unit 600 stores all the reagent components required by the corresponding analysis project. For example, in an analysis project, three reagent components including magnetic particles, label and dissociating agent must be allocated to the reactor 20, then the magnetic particles The three components, the label and the dissociating agent, are stored in the same storage unit 600. When a certain analysis project needs to load multiple reagent containers to expand the test volume of the project, the multiple reagent containers can be stored in each storage unit 600 in any suitable combination.
  • TSH thyroid stimulating hormone
  • all three TSH reagent containers can be loaded in the same storage unit 600. It is also possible to load one TSH reagent container in one storage unit 600 and the other two in the other storage unit 600.
  • the storage unit 610 can be driven to move to the designated liquid suction station, so that the reagent distribution member 500 can suck the reagent on the storage unit 610 at the liquid suction station and distribute it to the reactor 20.
  • At least one cavity of the reagent container on the storage unit 610 (for example, a magnetic particle cavity containing magnetic particle reagent components) is rotated around its own central axis , So that the magnetic particle reagent component in the form of a solid suspension generates a vortex, and prevents the solid matter (such as magnetic particles) from being precipitated.
  • a scanner can also be provided on the storage unit 600, and the scanner can identify the barcode information of the reagent container on the storage part 610, so as to distinguish different reagents.
  • a refrigerator may also be provided on the storage unit 600, and the refrigerator may perform refrigeration processing on the reagents in the storage part 610, so as to realize online long-term storage of the reagents.
  • the transfer unit 700 is used to transfer the reactor 20 between the ferry unit 200, the buffer unit 100 and the reaction device 12.
  • the transfer unit 700 can move horizontally and vertically. Obviously, the removal station 35 and the initial station 30 are both in transfer Above the movement trajectory of the unit 700.
  • the liquid distribution device 11 When the liquid distribution device 11 is used to distribute samples and reagents in the reactor 20 and mix the samples and reagents, a first liquid distribution method can be formed.
  • the main characteristics of the first liquid distribution method are: Distributed from the buffer unit 100 (not from the ferry unit 200) to the reactor 20.
  • the first liquid distribution method mainly includes the following steps:
  • S520 Stop the ferry unit 200 at the initial station 30, and transfer the reactor 20 to which the sample has been allocated from the buffer unit 100 to the ferry unit 200 at the initial station 30;
  • the ferry unit 200 is moved between the initial station 30 and the first station 31, and the reagent is distributed to the reactor 20 located at the first station 31; of course, the ferry unit 200 can be moved between the initial station 30 and the first station 31. Perform linear movement between the first stations 31. Wherein, when the reactor 20 carrying the distributed sample in the ferry unit 200 is moving or stationary, the sample can be distributed to the empty reactor 20 on the buffer unit 100 at the same time.
  • the ferry unit 200 is allowed to mix the sample and the reagents.
  • the ferry unit 200 can mix the reactants in the reactor 20 in a non-contact eccentric oscillating manner, thereby eliminating the carryover pollution of the reactants caused by other contact stirring.
  • the ferry unit 200 can mix the samples and reagents in the reactor 20.
  • the shortest time window in which the action sequence in the above steps S520, S530, S540, and S550 executed by the ferry unit 200 can be reproduced is recorded as the working period T, so that the ferry unit 200 works in one
  • Reactor 20 exits at position 30.
  • t ⁇ T more than one sample is distributed to the reactor 20 located on the buffer unit 100 in the interval of the working period T.
  • There is at least one t so that when t ⁇ T, the time for allocating samples to the empty reactor 20 on the cache unit 100 is not fixed, and it is not limited by the working period T.
  • the interval for allocating samples is determined according to the needs of the test, so that Testing is more flexible and efficient.
  • the sample is only distributed from the buffer unit 100 to the reactor 20, and not from the ferry unit 200 to the reactor 20, so that the distribution of samples from the ferry unit 200 to the reactor 20 can be omitted. Time, improve the working efficiency of the liquid distribution device.
  • the buffer unit 100 only includes the rotary disk 110 that moves in a circular motion or only the slider 120 that moves in a linear motion
  • the step of allocating samples to the buffer unit 100 includes the following sub-steps:
  • the reactor 20 follows the buffer unit 100 to move from the sample loading station 34 to the removal station 35, and the reactor 20 can be transferred from the removal station 35 to the ferry unit 200.
  • the step of distributing samples to the buffer unit 100 includes the following sub-steps:
  • the reactor 20 is transferred from the turntable 110 to the sliding block 120 of the buffer unit 100.
  • the reactor 20 follows the sliding block 120 to move linearly to the sample loading station 34, and distributes the sample to the reactor 20 located at the sample loading station 34 ;and
  • the reactor 20 follows the sliding block 120 to move linearly from the sample loading station 34 to the removal station 35, and the reactor 20 can be transferred from the removal station 35 to the ferry unit 200.
  • a reactor 20 whose reactants have been homogenized must be removed from the ferry unit 200 within a prescribed time. Therefore, when a single reactor 20 enters the ferry unit 200 until it leaves the ferry unit 200, the shorter the time (that is, the total residence time of the single reactor 20 on the ferry unit 200), the greater the test flux.
  • the residence time of the reactor 20 on the ferry unit 200 It will include at least sample distribution time, reagent distribution time, movement time of the ferry unit 200, and reactant mixing time.
  • the sample is only distributed from the buffer unit 100 to the reactor 20 (the sample is not distributed from the ferry unit 200 to the reactor 20), that is, the sample is only distributed in the reactor 20 of the buffer unit 100;
  • the reactor 20 containing the sample is transferred from the buffer unit 100 to the ferry unit 200, the reagent will be distributed to the reactor 20 on the ferry unit 200 that has already contained the sample. Therefore, compared with the traditional solution, on the basis of the same working efficiency of the sample addition piece 300 and the reagent distribution piece 500 and the same operating speed of the ferry unit 200, the residence time of the reactor 20 on the ferry unit 200 only includes reagent distribution.
  • the reactor 20 stays on the ferry unit 200 for a short time, so that the reactor 20 can remove the reactants in a relatively short time. Mixing and leaving the ferry unit 200 improves the test throughput of the entire immune analyzer 10.
  • the distribution of samples will not be restricted by the movement speed and position of the ferry unit 200.
  • the ferry unit 200 is moving or stationary.
  • the sample addition part 300 can make full use of the idle waiting time, thereby pre-allocating the samples from the buffer unit 100 to the reactor 20, compressing the total residence time of a single reactor 20 on the ferry unit 200, and finally improving the maximum test The purpose of flux. Therefore, even when the reagent distribution piece 500 distributes the reagents from the ferry unit 200 to the reactor 20 thereon, the sample addition piece 300 can distribute samples from the buffer unit 100 to the reactor 20 thereon, that is, samples and reagents.
  • the allocation can be performed synchronously, thereby eliminating the sample allocation time on the ferry unit 200.
  • the sample distribution cannot get rid of the restriction of the speed and position of the ferry unit 200. Only when the ferry unit 200 reaches the initial station 30, the sample 300 can be distributed from the swing unit to the reactor 20. For samples, it is impossible to distribute samples and reagents to the reactor 20 at the same time.
  • the operating speed (work efficiency or workload) of the ferry unit 200, the sample addition piece 300, and the reagent distribution piece 500 can be appropriately reduced. ), so as to reduce the difficulty of controlling the movement of the ferry unit 200, the sample adding piece 300 and the reagent distribution piece 500, and also reduce the vibration, noise and failure of the components in the liquid distribution device 11 due to high-speed operation, and improve the entire liquid The running stability and reliability of the distribution device 11.
  • a second liquid distribution method can also be formed.
  • the main features of the second liquid distribution method are: The same sample is sucked through the sample addition member 300 and distributed multiple times to at least two reactors 20; at the same time, after the same sample is absorbed and continuously distributed to at least two reactors, the sample addition member 300 is cleaned or replaced. The sample is continuously distributed between at least two reactors, and the sample addition part 300 is not cleaned or replaced.
  • the second liquid distribution method mainly includes the following steps:
  • the sample addition part 300 is provided, and the buffer unit 100 and the ferry unit 200 are provided at the same time.
  • the same sample is sucked through the sample addition member 300 and continuously distributed to at least two reactors 20 of the buffer unit 100, and the time interval between the continuous distribution of samples to at least two reactors 20 vacant on the buffer unit 100 is recorded. Assign an interval t to the sample.
  • the same sample here specifically refers to the same sample to be tested (that is, a sample corresponding to a certain subject, such as a sample of a certain patient) that needs to be tested at least twice, and the at least two tests can be at least two Different analysis items can also be the same analysis items repeated at least twice; different samples refer to samples from different subjects.
  • sample addition member 300 After the same sample is drawn and continuously distributed to at least two reactors, the sample addition member 300 is cleaned or replaced. When the same sample is drawn and continuously distributed between at least two reactors, the sample addition part 300 is not cleaned or replaced. In particular, between distributing different samples, the sample addition member 300 is cleaned or replaced.
  • S740 Record the shortest time window that can be cyclically reproduced by the ferry unit 200 as the working period T, and make the ferry unit 200 move between the initial station 30 and the first station 31, and transfer the reagents through the reagent distributor 500.
  • the first station 31 is distributed to each reactor 20 that has contained samples. Specifically, the reagent is distributed at the first station 31 to each of the reactors 20 on the ferry unit 200 through the reagent distribution member 500. Sample in the reactor 20. Similar to the ferry unit 200, the reagent distributing member 500 also distributes the reagents according to the working period T, that is, in each working period T, the reagent is distributed to only one reactor 20 containing the sample. Therefore, for at least two reactors after the sample adding member 300 distributes samples according to the sample distribution interval t, the interval time between which the reagent is distributed by the same reagent distribution member 500 is at least T.
  • the ferry unit 200 is allowed to mix the sample and the reagent.
  • the ferry unit 200 can mix the reactants in the reactor 20 in a non-contact eccentric oscillating manner, thereby eliminating the carryover pollution of the reactants caused by other contact stirring.
  • the ferry unit 200 can mix the samples and reagents in the reactor 20.
  • the reactor 20 is removed from the ferry unit 200 from the initial station 30 by the transfer unit 700 and transferred to the reaction device 12.
  • the sample adding member 300 adopts an elongated cylindrical sample needle, and after the same sample is drawn and continuously distributed to at least two reactors, in particular, the sample needle is moved to
  • the cleaning tank 800 cleans the inner wall and the outer wall of the sample needle at the same time to remove carryover contamination between different samples.
  • the cleaning fluid is injected into the inner cavity of the sample needle through a fluid power device such as a syringe or a pump.
  • the cleaning fluid flowing through the inner cavity of the sample needle at a certain speed will wash the inner wall of the sample needle to achieve a cleaning effect.
  • the outer wall of the sample needle is sprayed or immersed in a cleaning liquid to clean it, and the cleaning liquid flowing from the inner cavity and the outer wall of the sample needle can be discharged into the cleaning tank 800 at the same time.
  • the cleaning time of the sample needle is 2 to 10 seconds.
  • the sample addition member 300 adopts a disposable suction nozzle. After the same sample is sucked and continuously distributed to at least two reactors 20, the disposable suction nozzle is replaced. In particular, between distributing different samples, Replace the disposable nozzle. In this way, the cleaning of the disposable nozzle can be omitted, and the cleaning time can be reduced to improve efficiency. At the same time, the cost consumption of the disposable nozzle can be compensated by reducing the cost of cleaning liquid.
  • the sample addition member 300 when the sum of the samples required for all analysis items corresponding to the sample to be tested does not exceed the capacity of the sample needle, that is, when the capacity of the sample addition member 300 is greater than that of each reactor 20
  • the sample addition member 300 only sucks once and distributes the same sample to different reactors 20 in multiple consecutive times.
  • the sample addition piece 300 needs to draw the same blood sample (denoted as the first S) to detect two of the five items of A-function, namely the TSH item and the T4 item.
  • the reactor 20 For the reactor 20 that detects the TSH item, the reactor 20 (Denoted as TSH reactor) requires 100 microliters of blood sample; for the reactor 20 for testing T4 items, the reactor 20 (denoted as T4 reactor) requires 50 microliters of blood sample.
  • the volume of the sample addition piece 300 is greater than 150 microliters, that is, the volume of the sample addition piece 300 is greater than the sum of the blood sample volume required by the TSH reactor and the T4 reactor. Therefore, the sample addition piece 300 is disposable from the sample tube 430 Aspirate at least 150 microliters of blood sample (first S).
  • the buffer unit 100 drives the TSH reactor to move to the sample loading station 34, and the sample addition component 300 distributes 100 microliters of blood sample to the TSH reactor (first S) One S); then, the buffer unit 100 continues to drive the T4 reactor to the sample loading station 34, after the sample distribution interval t (there is at least one t such that t ⁇ T), the sample addition 300 distributes 50 to the T4 reactor Microliter blood sample (first S).
  • the sample addition member 300 can be moved above the cleaning tank 800 or in the cleaning tank 800 For backhaul calibration, you can also stay at sample loading station 34.
  • the reagent distributor 500 then distributes TSH reagents to the TSH reactor.
  • the TSH reagents may include R1 components and R2 components, and distribute T4 reagents to the T4 reactor after at least one working period T.
  • the T4 reagents may also include R1 components. Sub and R2 component.
  • the sample when the sum of the samples required for all analysis items corresponding to the sample to be tested exceeds the capacity of the sample needle, that is, when the capacity of the sample addition member 300 is less than the total amount of the same samples required by each reactor 20, the sample is added
  • the piece 300 draws the same sample (denoted as the second S) multiple times and distributes it to different reactors 20 according to the sample distribution interval t (there is at least one t such that t ⁇ T). For example, it is necessary to allocate the same sample (second S) to four reactors 20 to detect four different items.
  • the sample addition member 300 can be drawn twice to distribute the same sample to the four reactors 20 (second S). During operation, the sample addition part 300 sucks less than 500 microliters of sample for the first time (second S), and distributes it to the three reactors 20 according to the set amount for three consecutive times, and then, the sample addition part 300 second time Aspirate less than 500 microliters of sample and distribute it to the last reactor 20 in a set amount.
  • the sample adding piece 300 is moved into the cleaning tank 800 to reset the sample adding power unit. Since the reset needs to be carried out during the cleaning process of the sample addition piece 300, and there is no carryover problem between the same samples, only the sample addition piece 300 needs to be cleaned for a short period of time so that the sample addition piece 300 can be better completed. Prepare for the second sample draw.
  • the working efficiency of the sample addition part 300 is greater than the working efficiency of the reagent distribution part 500.
  • the reagent distribution member 500 can draw different reagent components multiple times and distribute them to the reactor 20 in each working period T.
  • the sample addition part 300 can distribute the samples to at least two reactors 20.
  • each reagent distribution piece 500 can only distribute each reagent component to one reactor 20, while the sample addition piece 300 can distribute samples to at least two reactors 20 .
  • the reagent distributing piece 500 absorbs different reagent components, the reagent distributing piece 500 is cleaned to prevent the carrying contamination of different reagent components; for example, the reagent components of different reagent components include R1 component and R2 component.
  • the accessory 500 sucks the R1 component and the R2 component into the same reactor 20 successively.
  • the reagent distribution piece 500 sucks the R2 component, the reagent distribution piece 500 that has just absorbed the R1 component is cleaned to prevent the R1 component
  • the R2 component constitutes carryover pollution.
  • the sample volume taken by the sample addition part 300 can be set to 10 to 500 ⁇ l each time, and the sample volume required for each reactor 20 is 5 to 250 ⁇ l.
  • the sample adding piece 300 needs to be cleaned, so as to eliminate the problems caused by the sample adding piece 300 extending into the sample tube 430 to suck the sample again and distribute the sample.
  • This will increase the number of cleanings, which will lead to at least the following three defects.
  • First there is a lot of time consumption, which also reduces the working efficiency of the sample addition part 300, thereby affecting the test throughput of the entire immune analyzer 10.
  • Second a large amount of cleaning solution is consumed, which increases the test cost of the immune analyzer 10.
  • the cleaning time of the sample addition piece 300 will be compressed, so that the sample addition piece 300 is not thoroughly cleaned and cannot effectively eliminate carryover pollution.
  • the second liquid distribution method has at least the following beneficial effects.
  • the sample adding piece 300 when the sample adding piece 300 is inserted into the sample tube 430 to absorb the same sample multiple times, the sample adding piece 300 only needs to be cleaned for a short time, so that the sample adding piece 300 can distribute a certain type of the same sample. , And then centrally allocate another same sample, reduce the frequency of switching between different samples, and further reduce the number of cleaning times and cleaning time of the sample addition 300. Third, since the number of cleaning times of the sample addition piece 300 is reduced, on the basis of ensuring work efficiency and test throughput, the cleaning time of each sample addition piece 300 can be appropriately extended, so that the sample addition piece 300 can be thoroughly cleaned. Effectively reduce the risk of contamination between different samples.
  • the reduction in the number of cleanings will also reduce the consumption of cleaning liquid, which can reduce the test cost of the immune analyzer 10.
  • the ferry unit 200 and the reagent distribution member 500 are coordinated and efficiently moved, which further improves the efficiency of reagent distribution and reactant mixing.
  • the reagent is distributed in the first station 31 by the reagent distributing member 500 to each reactor 20 that has contained the sample, and the sample and reagent are mixed by the ferry unit 200, and the sample is distributed Through the buffer unit, reagent distribution and reactant mixing are completed on the ferry unit, which improves the efficiency of sample, reagent distribution and reactant mixing, thereby improving the test efficiency and throughput of the immune analyzer.
  • the test throughput of the immune analyzer can break through the highest level currently reported in the industry (600 tests per hour), and achieve test throughput of 720, 800 tests per hour or even higher.
  • the diluent can be regarded as a component of the reagent corresponding to the analysis item
  • the sample and the diluent are mixed to form a diluted sample
  • the diluted sample is distributed to multiple reactors 20, and finally the reagents are distributed to the reactor 20 containing the diluted sample and mixed, which can form a sample dilution method.
  • the sample dilution method mainly includes the following steps:
  • S810 Move the first reactor carrying the sample in the ferry unit 200 from the initial station 30 to the first station 31.
  • S820 Distribute the diluent to the first reactor at the first station 31.
  • S840 Move the ferry unit 200 carrying the first reactor containing the diluted sample to the second station 32, and distribute the diluted sample in the first reactor at the second station to at least two vacant second stations.
  • the amount of diluted sample distributed to each vacant second reactor may be different.
  • the amount of diluted sample distributed in one of the second reactors may be greater than The amount of diluted sample dispensed in another second reactor.
  • the second reactor carrying the diluted sample in the ferry unit 200 is moved to the first station 31, and the reagent is distributed to the second reactor at the first station 31.
  • the first reactor is placed on the buffer unit 100, and after the sample is distributed in the first reactor on the buffer unit 100 through the sample addition member 300 , The first reactor containing samples is transferred from the buffer unit 100 to the ferry unit 200 at the initial station 30.
  • the samples are only distributed from the buffer unit 100 (not from the ferry unit 200) to the reactor 20, so that the distribution of samples will not be restricted by the speed and location of the ferry unit 200.
  • the ferry unit 200 carries the reaction
  • samples can be distributed from the buffer unit 100 (not from the ferry unit 200) to the reactor 20, thereby increasing the test throughput of the immune analyzer 10.
  • the empty second reactor is placed on the buffer unit 100.
  • the second reactor containing the diluted sample is removed from the buffer unit at the initial station 30.
  • 100 is transferred to the ferry unit 200.
  • the samples and diluents in the first reactor, and the diluted samples and reagents in the second reactor are all mixed by the ferry unit 200 itself; the method of mixing is non-contact eccentric shaking treatment.
  • the buffer unit 100 is moved between the receiving station 33, the sample loading station 34 and the removing station 35. Both the first reactor and the second reactor enter the buffer unit 100 from the receiving station 33, the sample is distributed from the sample loading station 34 to the first reactor, and the diluted sample is also distributed from the sample loading station 34 to the second reaction In the vessel, both the first reactor and the second reactor leave the buffer unit 100 from the removal station 35 and are transferred to the ferry unit 200.
  • the buffer unit 100 is the turntable 110
  • the turntable 110 drives the first and second reactors to make a circular motion between the receiving station 33, the sample adding station 34, and the removing station 35.
  • the buffer unit 100 is a slider 120
  • the slider 120 drives the first and second reactors to move linearly between the receiving station 33, the sample adding station 34, and the removing station 35.
  • the diluted sample formed by the liquid distribution device 11 can only be used by one reactor 20 each time, that is, the liquid distribution device 11 should perform a separate mixing process for each reactor 20 to form a dilution.
  • the sample in other words, the diluted sample formed by one mixing process corresponds to only one reactor 20. This increases the processing times of mixing to form a diluted sample, thereby reducing the working efficiency of sample dilution, and further affecting the maximum test throughput of the immune analyzer 10.
  • the liquid distribution device 11 mixes the sample and the diluent in the first reactor to form a diluted sample, and distributes the diluted sample in the first reactor to at least two second reactors. in. Therefore, the diluted sample formed by each mixing of the liquid distribution device 11 can be used by at least two reactors 20, which eliminates the need for the liquid distribution device 11 to perform a separate mixing process for each reactor 20 to form a diluted sample, in other words
  • the diluted sample formed by one mixing process can correspond to at least two reactors 20, which can greatly reduce the processing times of mixing to form a diluted sample, improve the working efficiency of the sample dilution method, and improve the maximum test pass of the immunoassay analyzer 10. the amount.
  • a third liquid distribution method can be formed.
  • the third liquid distribution method is similar to the first liquid distribution method, that is, the sample is not distributed from the swing unit to the reactor 20, but from the buffer unit 100 to the reactor 20.
  • the transfer unit transfers the reactor 20 containing the samples from the buffer unit 100 to the ferry unit 200.
  • the third liquid distribution method mainly includes the following steps:
  • S910 Provide at least two ferry units 200, so that each ferry unit 200 reciprocates between the initial station 30 and the first station 31.
  • S920 Transfer the reactor 20 containing the sample to the ferry unit 200 at the initial station 30.
  • the sample is distributed from the buffer unit 100 to the reactor 20.
  • the reactor 20 containing the sample is removed from the buffer unit 100 and moved to the ferry unit 200 at the initial station 30.
  • the ferry unit 200 drives the reactor 20 that is input from the buffer unit 100 and contains samples to move from the initial station 30 to the first station 31, and the reagents are distributed to the reactor 20 at the first station 31, and then The samples and reagents in the reactor 20 are mixed uniformly.
  • the length of the second cycle can be any suitable value within 4-15 seconds, such as 4 seconds, 4.5 seconds, 5 seconds, 6 seconds, 9 seconds, etc., corresponding to
  • the test throughput is 900-240 tests per hour, that is, the immune analyzer 10 can continuously report 900-240 results per hour. To facilitate understanding, the following takes 5 seconds for the second period as an example for description.
  • the ferry unit 200 must also output a reactor 20 that has been homogenized every 5 seconds. If there is only one ferry unit 200, the ferry unit 200 moves into the reactor 20 containing the sample, receives the reagent distributor 500 to dispense the reagents, eccentrically shakes and mixes, and removes and mixes is completed in one cycle. The total time required for the sequence of actions such as the reactor 20 is greater than 5 seconds.
  • the ferry unit 200 will not be able to output a mixed reactor 20 every 5 seconds.
  • the flow rate of the ferry unit 200 is lower than the outlet flow of the pipeline, resulting in The pipeline cannot work continuously at maximum efficiency (test throughput). Therefore, by setting the first period to twice the second period, that is, the first period is 10 seconds, and the number of ferry units 200 is set to two, the sequence of actions performed by the two ferry units 200 is relatively staggered in the second period.
  • the time (ie, 5 seconds) is executed, that is, the two ferry units 200 are "staggered in parallel" after a second cycle.
  • the reactor 20 containing the sample is transferred to the first ferry unit 200 at the 0th second, and then to the second ferry unit 200 at the 5th second
  • the reactor 20 containing the sample is transferred.
  • the reactor 20 on the first ferry unit 200 will output in the 10th second
  • the reactor 20 containing the sample will be transferred to the first swing unit.
  • the reactor 20 on the second ferry unit 200 will output at the 15th second.
  • the reactor 20 containing the sample will be transferred to the second swing unit.
  • the cycle operation will make the first ferry unit 200 output a reactor 20 at the 10th second, 20th second, 30th second,...10N second; meanwhile, the second ferry unit 200 will A reactor 20 is output at the 15th second, 25th second, 35th second, ... (5N+10) second. Therefore, on the basis that each ferry unit 200 outputs a reactor 20 that has been homogenized every 10 seconds, the two ferry units 200 as a whole will output a reactor that has been homogenized every 5 seconds. 20, so as to achieve the purpose of "quantity for time", and finally meet the requirements of the highest test throughput of the immune analyzer 10.
  • the time of the first period can be longer.
  • the number of ferry units 200 is three, four or more, and the first period can be set as the second period. Three times, four times or even more, that is, the first period is 15 seconds or 20 seconds, etc.
  • the movement speed of the ferry unit 200 can be reduced, the reagent distribution and the mixing time of samples and reagents can be prolonged, and the bottleneck of the movement speed of the ferry unit 200, reagent distribution and mixing of samples and reagents can be effectively solved.
  • Time bottleneck Under the condition that the moving speed of the ferry unit 200 and the mixing time of the sample and reagent are constant, each ferry unit 200 still outputs a reactor 20 whose mixing process is completed every 10 seconds, that is, the first cycle is still 10 seconds.
  • the third liquid distribution method further includes the following steps:
  • the storage units 600 equal to the number of the ferry units 200 are provided, and the reagents are stored in the multiple storage units 610 of each storage unit 600.
  • the storage unit 610 follows the storage unit 600 to move to the liquid suction station for sucking reagents.
  • the shortest time window in which the sequence of actions executed by each storage unit 600 can be reproduced cyclically is equal to the first period. From the first time when one of the storage units 600 drives the storage unit 610 to move towards the liquid suction station, it is staggered one second in sequence. The time interval between the cycles causes other storage units 600 to drive the storage unit 610 to move toward the corresponding liquid suction station.
  • the storage unit 610 is moved to the liquid suction station every other first cycle, when all the storage units 600 are regarded as a whole, every second cycle
  • the storage unit 610 will arrive at the liquid suction station at the time of, which can also achieve the purpose of “changing quantity for time”, and finally meet the requirement of the highest test throughput of the immune analyzer 10.
  • the number of storage units 600 is usually one.
  • the number of storage units 610 In order to increase the storage capacity of reagents for analysis items, the number of storage units 610 must be increased, which leads to an increase in the size of the entire storage unit 600.
  • the large occupied area is not conducive to the layout and manufacturing of the storage unit 600.
  • the storage unit 600 with a large volume and weight it also increases the difficulty of its motion control, which causes the storage unit 610 to fail in a short time. Arriving at the designated position for the reagent dispenser 500 to absorb reagents has become a bottleneck in achieving high test throughput. At the same time, when the storage unit 600 fails, the entire immune analyzer 10 will not work.
  • each storage unit 600 has a small volume, which is conducive to overall machine layout and motion control, and can also ensure a large reagent storage capacity.
  • the tolerance of the storage unit 600 to failure can be improved.
  • the other remaining storage units 600 can continue to work to ensure continuous supply of reagents.
  • the faulty storage unit 600 can be refurbished while other storage units 600 are working.
  • reagent dispensing parts 500 equal to the number of storage units 600 such that each storage unit 600 corresponds to one reagent dispensing part 500.
  • the shortest time window in which the sequence of actions performed by each reagent dispenser 500 can be reproduced cyclically is equal to the first cycle. From the time when one of the reagent dispensers 500 dispenses reagents, the time interval of the second cycle is sequentially staggered to make other reagents The sub-assembly 500 dispenses reagents.
  • the number of reagent dispensers 500 and storage units 600 and the number of ferry units 200 are equal, and at the same time
  • the reagent distributor 500 is also "staggered in parallel” every second cycle.
  • all the reagent dispensing pieces 500 as a whole one of the reagent dispensing pieces 500 will dispense reagents every second period of time.
  • the reagent distributing part 500 will dispense the reagent once, which can also achieve the purpose of “changing the quantity for time”, and finally meet the requirement of the highest test throughput of the immune analyzer 10.
  • the storage unit 600 can be divided into two equal halves. One half of the storage unit 600 is opposite to the other half of the storage unit 600.
  • the trajectory of the movement is symmetrical, which is beneficial to the layout of the immune analyzer 10 as a whole.
  • the reactor 20 containing the sample is distributed from the buffer unit 100 to the reactor 20 in advance, eliminating the need for the ferry unit 200 to the reactor 20.
  • the time for distributing samples in the medium thereby reducing the residence time of the reactor 20 on each ferry unit 200, so that each ferry unit 200 can quickly output one reactor 20.
  • the number of ferry units 200 is set to at least two, and at least two ferry units 200 are "staggered in parallel" after a second cycle.
  • the third liquid distribution method is similar to the second liquid distribution method, that is, the same sample is sucked by the sample addition member 300 and continuously distributed to at least two reactors 20 on the buffer unit 100. At the same time, after the same sample is drawn and continuously distributed to at least two reactors, the sample addition member 300 is cleaned or replaced.
  • the second liquid distribution method please refer to the description of the second liquid distribution method.
  • the sample injection part 300 is made to adopt a sample needle or a disposable suction nozzle. After the same sample is drawn and distributed to at least two reactors continuously, the inner and outer walls of the sample needle are cleaned or once The sex nozzle is replaced. For thorough cleaning, the cleaning time for the sample needle is 2 to 10 seconds.
  • the capacity of the sample adding member 300 is greater than the total amount of the same sample required by each reactor 20, the sample adding member 300 only sucks once and distributes the same sample to different reactors 20 in multiple consecutive times.
  • the sample addition part 300 sucks the same sample once (first S) and distributes the first S divided into four parts (denoted as S1, S2, S3, S4, respectively) into the four reactors in four consecutive times. Since the next sample taken by the sample addition piece 300 is a different sample, after allocating S1, S2, S3, S4 to the four reactors, clean the inner and outer walls of the sample needle of the sample addition piece 300 or clean the disposable nozzle Replace it.
  • each reagent distribution piece 500 distributes the reagent corresponding to the analysis item in the first cycle to the first S that has been allocated on the ferry unit 200 at the first station.
  • the two reagent distributors 500 are separated by N second cycles to distribute the reagents corresponding to the analysis items to the first S-allocated reactor on the ferry unit 200 at the ferry position at the first station 31 Inside.
  • the first reagent distribution member 500 distributes the reagent to the first reactor 20 containing S1 in the first first period T, and distributes the reagent to the third reactor 20 containing S3 in the second first period T.
  • the second reagent distribution piece 500 distributes reagents to the second reactor 20 containing S2 in the second first cycle T, and distributes reagents to the fourth reactor 20 containing S4 in the third first cycle T. It can be seen that the first reagent The sub-assembly 500 and the second reagent distributing member 500 are separated by N second cycles (T/2) to distribute the reagents corresponding to the analysis items. In this way, it can be ensured that each second period (T/2) has a reactor 20 to complete the distribution of reagents, thereby improving the efficiency of reagent distribution.
  • each reagent distributing piece 500 is sequentially staggered for a second cycle (T/2) and the analysis items are distributed at intervals of N (N is an integer and N ⁇ 1) first cycle.
  • T/2 the second cycle
  • N is an integer and N ⁇ 1 first cycle.
  • the third liquid distribution method of the present application improves the sample distribution efficiency and effectively reduces the pollution carried between samples, so that at least two ferry units 200, at least two storage units 600, and at least two reagent distribution pieces 500 Efficient and coordinated movement further improves the efficiency of reagent distribution and reactant mixing, thereby improving the test efficiency and throughput of the immune analyzer.
  • the test throughput of the immune analyzer of this application can break through the highest reported in the industry. Level (600 tests per hour) to achieve test throughput of 720, 800 tests per hour or even higher.
  • This application also provides an immunoassay method, which includes the steps in the first liquid distribution method, the second liquid distribution method, the third liquid distribution method, and the sample dilution method described above.

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Abstract

La présente invention concerne un procédé de distribution de liquide, comprenant les étapes suivantes : fournir une unité tampon (100) utilisée pour recevoir un réacteur (20) et amener ledit réacteur (20) à se déplacer, et utilisée pour distribuer un échantillon dans un réacteur vide (20) situé sur l'unité tampon (100) ; transférer de l'unité tampon (100) à une unité de convoyage (200) située au niveau d'un poste de travail initial (30) le réacteur (20) contenant un échantillon distribué ; et amener l'unité de convoyage (200) à se déplacer entre le poste de travail initial (30) et un premier poste de travail (31), et distribuer un réactif dans le réacteur (20) situé au niveau du premier poste de travail (31) ; un échantillon pouvant également être distribué dans un réacteur vide (20) sur l'unité tampon (100) pendant le processus de déplacement ou d'arrêt de l'unité de convoyage (200) portant un réacteur (20) contenant un échantillon distribué.
PCT/CN2019/082166 2019-02-02 2019-04-11 Procédé de distribution de liquide et procédé de dosage immunologique WO2020155385A1 (fr)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112268869A (zh) * 2020-10-20 2021-01-26 深圳立勤生物科技有限公司 一种用于农药残留检测仪的检测方法及相应的检测仪

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080206097A1 (en) * 2003-11-25 2008-08-28 Katsuaki Takahashi Automatic analyzer
CN102590539A (zh) * 2012-02-22 2012-07-18 漯河曙光汇知康生物科技有限公司 分立式全自动生化分析仪
CN103760375A (zh) * 2014-01-28 2014-04-30 桂林优利特医疗电子有限公司 改进的自动生化分析仪及其使用方法
CN205656200U (zh) * 2016-03-04 2016-10-19 深圳普门科技有限公司 一种高速c反应蛋白分析仪
CN206740769U (zh) * 2017-03-02 2017-12-12 重庆科斯迈生物科技有限公司 免疫分析仪反应杯条移送系统
CN207866714U (zh) * 2017-12-28 2018-09-14 深圳市新产业生物医学工程股份有限公司 化学发光检测仪

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69928644T2 (de) * 1999-05-14 2006-07-27 F. Hoffmann-La Roche Ag Automatisches Analysesystem
CN203825021U (zh) * 2014-05-21 2014-09-10 嘉兴凯实生物科技有限公司 一种条式免疫全自动分析仪用板条进给装置
CN207472774U (zh) * 2017-09-29 2018-06-08 深圳市新产业生物医学工程股份有限公司 化学发光检测仪及其温育装置
CN207472773U (zh) * 2017-09-29 2018-06-08 深圳市新产业生物医学工程股份有限公司 化学发光检测仪、温育装置及反应盘机构
CN207516251U (zh) * 2017-09-29 2018-06-19 深圳市新产业生物医学工程股份有限公司 化学发光检测仪、温育装置及反应盘机构
CN207866716U (zh) * 2017-12-28 2018-09-14 深圳市新产业生物医学工程股份有限公司 化学发光检测仪
CN207866715U (zh) * 2017-12-28 2018-09-14 深圳市新产业生物医学工程股份有限公司 化学发光检测仪

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080206097A1 (en) * 2003-11-25 2008-08-28 Katsuaki Takahashi Automatic analyzer
CN102590539A (zh) * 2012-02-22 2012-07-18 漯河曙光汇知康生物科技有限公司 分立式全自动生化分析仪
CN103760375A (zh) * 2014-01-28 2014-04-30 桂林优利特医疗电子有限公司 改进的自动生化分析仪及其使用方法
CN205656200U (zh) * 2016-03-04 2016-10-19 深圳普门科技有限公司 一种高速c反应蛋白分析仪
CN206740769U (zh) * 2017-03-02 2017-12-12 重庆科斯迈生物科技有限公司 免疫分析仪反应杯条移送系统
CN207866714U (zh) * 2017-12-28 2018-09-14 深圳市新产业生物医学工程股份有限公司 化学发光检测仪

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112268869A (zh) * 2020-10-20 2021-01-26 深圳立勤生物科技有限公司 一种用于农药残留检测仪的检测方法及相应的检测仪

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