WO2020151602A1 - 一种用于生物反应器的组合型温度计和生物反应器 - Google Patents

一种用于生物反应器的组合型温度计和生物反应器 Download PDF

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Publication number
WO2020151602A1
WO2020151602A1 PCT/CN2020/072882 CN2020072882W WO2020151602A1 WO 2020151602 A1 WO2020151602 A1 WO 2020151602A1 CN 2020072882 W CN2020072882 W CN 2020072882W WO 2020151602 A1 WO2020151602 A1 WO 2020151602A1
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Prior art keywords
thermometer
sheath body
sealing ring
sleeve
bioreactor
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Ceased
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PCT/CN2020/072882
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English (en)
French (fr)
Inventor
张明
唐寅
曾良平
周珍
郭俊福
施雪克
郭谋之
张鑫
孔乾
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Qingdao Innovation Institute Of East China University Of Science And Technology
Shanghai Guoqiang Biochemical Engineering Equipment Co Ltd
East China University of Science and Technology
Original Assignee
Qingdao Innovation Institute Of East China University Of Science And Technology
Shanghai Guoqiang Biochemical Engineering Equipment Co Ltd
East China University of Science and Technology
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Application filed by Qingdao Innovation Institute Of East China University Of Science And Technology, Shanghai Guoqiang Biochemical Engineering Equipment Co Ltd, East China University of Science and Technology filed Critical Qingdao Innovation Institute Of East China University Of Science And Technology
Publication of WO2020151602A1 publication Critical patent/WO2020151602A1/zh
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
    • G01K15/00—Testing or calibrating of thermometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
    • G01K5/00—Measuring temperature based on the expansion or contraction of a material
    • G01K5/02—Measuring temperature based on the expansion or contraction of a material the material being a liquid
    • G01K5/04—Details

Definitions

  • the utility model relates to the field of biotechnology, in particular to a combined thermometer and a bioreactor used in a bioreactor.
  • the temperature of the culture solution directly affects the process of various biological and biochemical reactions and the characteristics of the material in the metabolic process, and has a direct impact on the culture results and the components of the metabolites; in addition, it is reliable for the sterilization of the bioreactor Sex has a direct impact. Therefore, the accuracy of the temperature detection of the culture fluid in the bioreactor is very important for the product quality control of the biopharmaceutical production process. Therefore, the temperature detection system of the culture fluid needs to be calibrated frequently during the installation, commissioning and use of the bioreactor to ensure the accuracy of the temperature control results and ensure product quality.
  • the liquid level of the tested liquid in the bioreactor will form a certain liquid level static pressure, and the bioreactor needs to be maintained at 0.1 during in-situ steam sterilization.
  • the tank pressure of MPa or even higher requires the pressure-bearing and temperature-resistant seal between the glass standard thermometer and the tank body when the glass standard thermometer is inserted into the tank during the calibration process.
  • the glass standard thermometer material is easy to break, it is difficult to form a reliable structure with a pressure-bearing and temperature-resistant seal with the metal.
  • the usual method is to open an interface on the bioreactor that needs temperature calibration and use a metal with a front section closed.
  • the casing is inserted into the tank, and the part remaining on the outside of the tank is fixed and sealed with a sealing structure; then a standard thermometer is inserted into the casing, and the temperature electrode is calibrated by reading the reading of the standard thermometer.
  • the purpose of the utility model is to provide a combined thermometer and bioreactor used in a bioreactor.
  • the glass bulb end of the thermometer can be directly arranged in the bioreactor and directly contact the liquid in the bioreactor to improve Improve the detection sensitivity and accuracy of the thermometer, thereby improving the calibration and measurement accuracy of the temperature detection system.
  • a combined thermometer for a bioreactor comprising: a sheath body, a mounting hole is opened in the sheath body, and a thermometer is arranged in the mounting hole; one end of the sheath body is also provided with a first A sealing ring, the inner side of the first sealing ring abuts against the thermometer, and the outer side of the first sealing ring abuts against the sheath main body; an end of the sheath main body close to the first sealing ring is provided There is a locking member, the locking member is provided with a locking hole for the thermometer to pass through; the locking member is used to sleeve the sheath body and press the first sealing ring to The sheath body and the thermometer.
  • the first sealing ring can abut between the thermometer and the sheath body, and the sealing requirement between the thermometer and the sheath body is achieved; by setting the thermometer In the main body of the sheath with both ends open, the glass bulb end of the thermometer can be directly set in the bioreactor and make direct contact with the liquid in the bioreactor, which improves the detection sensitivity and accuracy of the thermometer, thereby improving Calibration and measurement accuracy of temperature detection system.
  • thermometer further includes a second sealing ring sleeved on the thermometer, and the outer side of the second sealing ring abuts against the sheath body.
  • a pressure sleeve is sheathed on the outer side of the thermometer, and a pressure groove communicating with the mounting hole is opened in the sheath body; one end of the pressure sleeve abuts against the first sealing ring, and the pressure The other end of the sleeve abuts against the second sealing ring.
  • the restriction between the first sealing ring, the second sealing ring and the thermometer is increased.
  • the pressure sleeve can limit the sealing ring, reducing the possibility of the thermometer shaking and increasing the thermometer The stability.
  • the end of the locking member away from the sheath body is further provided with a sleeve, and a connecting groove for the thermometer to pass through is provided in the sleeve; the glass bulb end of the thermometer is arranged at the end of the sheath body away from One end of the sleeve and the other end of the thermometer are embedded in the sleeve.
  • thermometer exposed on the outside of the bioreactor is protected through the setting of the sleeve, which reduces the possibility of damage to the thermometer.
  • an end of the sheath body away from the sleeve is provided with an adjustment window, and the adjustment window is opened on the sheath body along the length direction of the sheath body.
  • the glass bulb end of the thermometer can usually be set at the position of the adjustment window.
  • the user can directly observe the position of the thermometer in the main body of the sheath, and adjust the insertion depth of the thermometer in the bioreactor.
  • an observation window is opened on the sleeve, and the observation window is opened in the sleeve along the length direction of the sleeve.
  • the user can notify the observation window to directly observe the scale of the thermometer without removing the sleeve. While protecting the thermometer, the temperature value of the thermometer can also be read, which is convenient for the user's operation.
  • a fixing member for abutting against the reactor body is sleeved on the sheath body, and a side of the fixing member close to the locking member is arranged on the outside of the reactor body, and the fixing member The side away from the locking member is arranged in the reactor body.
  • an outer sealing ring is provided on the sheath body on the side of the fixing member away from the locking member, and the outer side of the outer sealing ring is used to abut against the inner wall of the reactor body.
  • the sheath main body and the bioreactor are sealed by the arrangement of the outer sealing ring.
  • a pressure ring is provided between the sheath body and the locking member; the locking member is also used to compress the pressure ring to the sheath body, and to compress the first sealing ring Between the pressure ring and the sheath body.
  • One of the objectives of the present invention is to provide a bioreactor, including a reactor body, the reactor body is provided with an interface, and a combined thermometer for the bioreactor is installed on the interface.
  • the first sealing ring can abut between the thermometer and the sheath body, and the sealing requirement between the thermometer and the sheath body is achieved; by setting the thermometer In the main body of the sheath with both ends open, the glass bulb end of the thermometer can be directly set in the bioreactor and make direct contact with the liquid in the bioreactor, which improves the detection sensitivity and accuracy of the thermometer, thereby improving Calibration and measurement accuracy of temperature detection system.
  • thermometer and bioreactor used in the bioreactor provided by the present invention have the following beneficial effects:
  • thermometer can be directly set in the bioreactor and directly contact the liquid in the bioreactor, which improves the detection sensitivity and accuracy of the thermometer, thereby improving the calibration and measurement accuracy of the temperature detection system .
  • the pressure sleeve can limit the sealing ring, reducing the possibility of the thermometer shaking and increasing the stability of the thermometer .
  • the setting of the first sealing ring and the second sealing ring, as well as the locking part and the pressure sleeve limit the sealing ring, reduce the possibility of the shaking of the thermometer and increase the stability of the thermometer; at the same time, the addition of the second sealing ring And the second sealing ring abuts on the inner wall of the thermometer and the pressure groove, that is, the main body of the sheath, which realizes the second sealing between the thermometer and the main body of the sheath, and increases the reliability of the sealing of the bioreactor.
  • thermometer for a bioreactor and a bioreactor
  • Figure 1 is a schematic structural diagram of a combined thermometer used in a bioreactor according to the present invention.
  • a combined thermometer for a bioreactor includes a sheath body 10, the sheath body 10 is in a long cylindrical shape, and the sheath body 10 is A mounting hole 11 is provided in the sheath body 10 along its length, and both ends of the mounting hole 11 penetrate the sheath body 10; the mounting hole 11 is provided with a thermometer 20, which can be removed from the sheath body One end of 10 penetrates into the sheath body 10.
  • first sealing ring 31 is provided at the left end of the sheath body 10; the first sealing ring 31 is ring-shaped, and the inner side of the first sealing ring 31 abuts Connected to the thermometer 20, the outer side of the first sealing ring 31 abuts against the sheath body 10, that is, one end of the sheath body 10 is clamped between the sheath body 10 and the thermometer 20 by the first sealing ring 31 to realize the protection The seal between the main body and the thermometer.
  • One end of the sheath body 10 close to the first sealing ring 31 is provided with a locking member 40, that is, the locking member 40 is provided on the left side of the first sealing ring 31; the locking member 40 is provided with a locking member for the thermometer 20 to pass through.
  • Hole 41, the locking member 40 can be sleeved on the sheath body 10, and the first sealing ring 31 can be pressed between the sheath body 10 and the thermometer 20, that is, the locking member 40 can be at one end of the sheath body 10
  • the locking member 40 can press the first sealing ring 31 on the sheath body 10 and the thermometer 20.
  • the glass bulb end of the thermometer 20 can be arranged at any end of the sheath body 10, that is, the glass bulb of the thermometer 20 can be arranged at the left or right end of the sheath body 10.
  • the first sealing ring 31 can abut between the thermometer 20 and the sheath main body 10, realizing the connection between the sheath main body 10 and the thermometer 20 Sealing requirements; by placing the thermometer 20 in the sheath body 10 with both ends open, that is, the glass bulb end of the thermometer 20 can be directly set in the reactor body and directly contact the liquid in the reactor body, adding a thermometer 20 detection sensitivity and accuracy, thereby improving the calibration and measurement accuracy of the temperature detection system.
  • a pressure ring 50 is provided between the sheath body 10 and the locking member 40, and the locking member 40 is also used to compress the pressure ring 50 to the sheath body 10 and press the first sealing ring 31 to the pressure Between the ring 50 and the sheath body 10; that is, when the locking member 40 is arranged on the sheath body 10, the pressure ring 50 can be pressed against the sheath body 10, and the first sealing ring 31 can be pressed against the pressure ring 50 and the sheath Between the main body 10 and the thermometer 20.
  • the pressure ring 50 Through the arrangement of the pressure ring 50, the pressure ring 50 and the thermometer 20 can be well sealed, which further achieves the sealing requirements of the bioreactor.
  • thermometer 20 is a standard part
  • the side of the sheath body 10 close to the pressure ring 50 is provided with a first pressing surface
  • the side of the pressure ring 50 near the sheath body 10 is provided with a second pressing surface.
  • the second pressing surface communicates with the first pressing surface
  • the first pressing surface and the second pressing surface can be formed by chamfering, and the sheath body 10, the pressing ring 50 and the thermometer 20 can form a space for accommodating the first pressing surface.
  • the first sealing ring 31 abuts between the sheath body 10 and the thermometer 20 to achieve the first seal between the thermometer 20 and the sheath body 10.
  • a sealing ring 31 abuts between the thermometer 20 and the pressure ring 50 to achieve a second seal between the thermometer 20 and the sheath body 10.
  • the locking member 40 can be connected to the sheath body 10 in any connection manner such as snap connection, snap connection, or threaded connection.
  • the locking member 40 is a locking nut, and the locking member 40 is connected by a thread.
  • the sheath body 10 through the threaded connection between the locking member 40 and the sheath body 10, the locking and releasing of the locking member 40 is facilitated.
  • the first sealing ring 31 can always tightly abut on the thermometer 20; further, when the locking member 40 is locked When tightly on the sheath body 40, the locking assembly 40 can also increase the constraint between the thermometer 20 and the sheath body 10, reduce the possibility of the thermometer 20 sliding along its axis, and increase the thermometer 20 in the sheath body 10. On the stability. When the position of the thermometer 20 needs to be adjusted, the locking member 40 is released to release the constraint between the thermometer 20 and the sheath body 10, so that the sliding movement of the thermometer 20 can be realized without completely releasing the locking member 40, and the operation is more convenient.
  • thermometer used in a bioreactor.
  • the difference between this embodiment and the first embodiment lies in the addition of a second sealing ring 32.
  • this embodiment further includes a second sealing ring 32 sleeved on the thermometer 20.
  • the outer side of the second sealing ring 32 abuts against the sheath body 10;
  • the addition of 32 realizes the second seal between the thermometer 20 and the sheath body 10, and increases the reliability of the seal.
  • the sheath body 10 is provided with a pressure groove 12 for setting the second sealing ring 32, the inner side of the second sealing ring 32 can abut on the thermometer 20, and the outer side of the second sealing ring 32 abuts against the pressure groove 12 On the inner wall.
  • the outer side of the thermometer 20 is sleeved with a pressure sleeve 60, and the sheath body 10 is provided with a pressure groove 12 communicating with the mounting hole 11; one end of the pressure sleeve 60 can abut against the first sealing ring 31, the pressure sleeve 60 The other end can abut against the second sealing ring 32.
  • the compression sleeve 60 can limit the sealing rings (31, 32) and reduce the shaking of the thermometer 20 The possibility of increasing the stability of the thermometer 20.
  • the first compression surface can be provided on the sheath body 10, the first sealing ring 31 can directly abut on the pressure ring 50, the sheath body 10, the thermometer 20 and the pressure sleeve 60, the first sealing ring 31 can realize the sealing between the thermometer 20 and the sheath body 10; or directly
  • a third pressing surface is provided at one end of the pressing sleeve 60 close to the first sealing ring 31.
  • the second pressing surface and the third pressing surface form a space for accommodating the first sealing ring 31, and the first sealing ring 31 can directly abut Connected to the pressure ring 50, the thermometer 20 and the pressure sleeve 60, the first sealing ring 31 can achieve a double seal between the thermometer 20 and the sheath body 10, and an appropriate sealing method can be selected according to the needs of the user.
  • the second sealing ring 32 can also stably abut on the thermometer 20, and at the same time ensure sealing and restraint of the thermometer 20 from sliding.
  • thermometer used in a bioreactor.
  • the difference between this embodiment and the first embodiment is the addition of a sleeve 70.
  • the end of the locking member 40 away from the sheath body 10 is provided with a sleeve 70, and the sleeve 70 is provided with a connecting groove for the thermometer 20 to pass through.
  • the glass bulb end can be set at the end of the sheath away from the sleeve 70, and the other end of the thermometer 20 is embedded in the sleeve 70, that is, the sleeve 70 can be set at the left end of the locking member 40; through the setting of the sleeve 70, The sleeve 70 exposed on the outside of the reactor body is protected, which reduces the possibility of damage to the thermometer 20.
  • the sleeve 70 can be directly integrally formed, and a connecting groove is opened on the right side of the sleeve 70; or the sleeve 70 selects a pipe with open ends at both ends, and adds a cover plate at the left end opening of the pipe to form a connecting groove;
  • the tube 70 can be connected to the locking member 40 in any connection manner such as a snap connection, a snap connection, or a threaded connection.
  • the sleeve 70 is threaded to the locking member 40 to facilitate the installation and removal of the sleeve 70.
  • the end of the sheath body 10 away from the sleeve 70 is provided with an adjusting window 13, that is, the adjusting window 13 is provided at the right end of the sheath body 10, and the adjusting window 13 is opened on the sheath body 10 along the length direction of the sheath body 10.
  • the glass bulb end of the thermometer 20 can be positioned at the adjustment window 13.
  • the user can directly observe the position of the thermometer 20 in the sheath body 10 and adjust the insertion depth of the thermometer 20 in the reactor body.
  • To detect the temperature of the liquid in the tank at a suitable position, while protecting the thermometer facilitate the flow of the liquid in the reactor at the glass bulb end of the thermometer, and improve the sensitivity and accuracy of temperature detection.
  • the sleeve 70 is further provided with an observation window 71, which can be opened on the sleeve 70 along the length direction of the sleeve 70.
  • the observation window 71 has a waist-shaped hole, and the user can notify the observation window 71 to directly observe the thermometer.
  • the scale of 20 does not need to remove the sleeve 70. While protecting the thermometer 20, the temperature value of the thermometer 20 can be read, which is convenient for the user's operation.
  • thermometer 20 protruding outside the bioreactor, and the heat preservation device can be arranged on the sheath body 10 or the sleeve 70 , Through the setting of the heat preservation device, the thermometer can be tested under the conditions that are more in line with the requirements of standard thermometers, and the detection accuracy of the thermometer is further improved.
  • thermometer 80 used in a bioreactor.
  • the difference between this embodiment and the first embodiment lies in the addition of a fixing member 80.
  • the sheath body 10 is provided with a fixing member 80 for abutting against the reactor body, and the fixing member 80 can be arranged on the side of the reaction vessel close to the locking member 40.
  • the fixing member 80 is arranged in the reactor body on the side away from the locking member 40. Therefore, the locking member 40 can be arranged on the outside of the reactor body.
  • the reactor body is realized by the fixing member 80.
  • the fixing member 80 is threadedly connected to the sheath body 10 to facilitate pressing the sheath body 10 onto the reactor body.
  • the reactor body is provided with an interface, the end of the sheath body 10 close to the glass bulb of the thermometer 20 can pass through the interface and enter the reactor body, and the fixing member 80 can abut on the reactor body outside the interface.
  • the side of the fixing member 80 away from the locking member 40 is provided with an outer sealing ring 33.
  • the outer sealing ring 33 can abut on the interface of the reactor.
  • the arrangement of the outer sealing ring 33 further increases the contact with the bioreactor.
  • the degree of sealing increases the reliability of the sealing of the bioreactor.
  • the outer sealing ring 33 can abut on the inner wall of the reactor body interface.
  • a bioreactor includes:
  • the reactor body (not shown in the figure) is provided with an interface on the reactor body, and the combined thermometer for the bioreactor described in any of the above embodiments is installed on the interface.

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  • General Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

本实用新型公开了一种用于生物反应器的组合型温度计和生物反应器，涉及生物技术领域。包括：护套主体，所述护套主体内开设有安装孔，所述安装孔内设有温度计；所述护套主体的一端还设有第一密封圈，所述第一密封圈的内侧抵接于所述温度计，所述第一密封圈的外侧抵接于所述护套主体；所述护套主体靠近所述第一密封圈的一端设有锁紧件，所述锁紧件上设有用于温度计穿过的锁紧孔；所述锁紧件用于套设在所述护套主体上，并将所述第一密封圈压紧于所述护套主体与所述温度计。温度计的玻璃泡端能够直接设置在生物反应器内，并与生物反应器内的液体进行直接接触，增加了温度计的检测灵敏度和准确度，提高了温度检测系统的标定与测量准确性。

Description

一种用于生物反应器的组合型温度计和生物反应器 技术领域
本实用新型涉及生物技术领域，尤指一种用于生物反应器的组合型温度计和生物反应器。
背景技术
近年来，生物制药产业在国民经济中发挥的作用越来越大，同时国家对药品生产的质量控制要求也日趋严格。
在微生物培养过程中，培养液的温度直接影响了代谢过程中各种生物与生化反应进程及料液特性，对培养结果及代谢产物组分有直接影响；此外还对生物反应器的灭菌可靠性有直接影响。因此，生物反应器内培养液的温度检测的准确性，对生物制药的生产过程产品质量控制来说至关重要。所以生物反应器在安装调试与使用过程中需要经常对培养液的温度检测系统进行标定，以保证温度控制结果的准确性，保证产品质量。
检测温度的标定有多种方法，其中最可靠、为监管部门与反应器使用单位共同接受的方法，是采用标准玻璃温度计检测反应器内液体的温度作为标准值，对反应器温度测控系统进行标定。
在实际应用中，由于需要将标准温度计置于被检测液中，生物反应器被检测液的液位高度会形成一定的液位静压，加上生物反应器原位蒸汽灭菌时需要维持0.1MPa甚至更高的罐压，就要求在标定过程中保证玻璃标准温度计插入罐内时与罐体之间的承压耐温的密封。
由于玻璃标准温度计材质容易碰碎，与金属之间形成承压耐温密封的可靠结构较为困难，通常的方法是在需要进行温度标定的生物反应器上开一个接口，采用一根前段封闭的金属套管插入罐内，留在罐体外侧的部分与罐体采用 密封结构进行固定与密封；之后在套管内插入标准温度计，通过读取标准温度计的读数对温度电极进行标定。
这种方法虽然解决了承压耐温与密封要求，但由于采用了金属套管，使标准温度计与被检测的液体只能进行间接测量，标准温度计的玻璃泡端与金属套管只有点接触，其余部分存在空气层，降低了标准温度计的检测灵敏度和准确度，温度检测系统的标定与测量准确性较低。
发明内容
本实用新型的目的是提供一种用于生物反应器的组合型温度计和生物反应器，温度计的玻璃泡端能够直接设置在生物反应器内，并与生物反应器内的液体进行直接接触，提高了温度计的检测灵敏度和准确度，从而提高了温度检测系统的标定与测量准确性。
本实用新型提供的技术方案如下：
一种用于生物反应器的组合型温度计，包括：护套主体，所述护套主体内开设有安装孔，所述安装孔内设有温度计；所述护套主体的一端还设有第一密封圈，所述第一密封圈的内侧抵接于所述温度计，所述第一密封圈的外侧抵接于所述护套主体；所述护套主体靠近所述第一密封圈的一端设有锁紧件，所述锁紧件上设有用于温度计穿过的锁紧孔；所述锁紧件用于套设在所述护套主体上，并将所述第一密封圈压紧于所述护套主体与所述温度计。
本技术方案中，通过锁紧件与第一密封圈的设置，第一密封圈能够抵接在温度计与护套主体之间，实现了温度计与护套主体之间的密封要求；通过将温度计设置在两端开口的护套主体内，使温度计的玻璃泡端能够直接设置在生物反应器内，并与生物反应器内的液体进行直接接触，提高了温度计的检测灵敏度和准确度，从而提高了温度检测系统的标定与测量准确性。
进一步，还包括套设在所述温度计上的第二密封圈，所述第二密封圈的外 侧抵接于所述护套主体。
本技术方案中，通过第二密封圈的添加，实现了温度计与护套主体之间的第二次密封，增加了密封可靠性。
进一步，所述温度计的外侧套设有压套，所述护套主体内开设有与所述安装孔连通的压槽；所述压套的一端抵接于所述第一密封圈，所述压套的另一端抵接于所述第二密封圈。
本技术方案中，通过压套的设置，增加了第一密封圈、第二密封圈与温度计之间的约束，压套能够对密封圈进行限位，减小了温度计晃动的可能，增加了温度计的稳定性。
进一步，所述锁紧件远离所述护套主体的一端还设有套管，所述套管内设有用于温度计穿过的连接槽；所述温度计的玻璃泡端设置在所述护套主体远离所述套管的一端，所述温度计的另一端嵌设于所述套管内。
本技术方案中，通过套管的设置，对裸露在生物反应器外侧的温度计进行保护，减小了温度计损坏的可能。
进一步，所述护套主体远离所述套管的一端设有调节窗口，所述调节窗口沿所述护套主体的长度方向开设于所述护套主体。
本技术方案中，温度计的玻璃泡端通常能够设置在调节窗口的位置，通过调节窗口的设置，用户便于直接观察到温度计在护套主体内的位置，便于调节温度计在生物反应器内的插入深度，在合适的位置检测罐体内液体温度，在保护温度计的同时，便于反应器内液体在温度计玻璃球端的流动，提高温度检测的灵敏度准确性。
进一步，所述套管上还开设有观察窗口，所述观察窗口沿所述套管的长度方向开设于所述套管。
本技术方案中，用户能够通知观察窗口直接观察到温度计的刻度，而无需取下套管，在保护温度计的同时，还能够实现温度计的温度数值读取，便于用 户的操作。
进一步，所述护套主体上套设有用于抵接在反应器本体上的固定件，所述固定件靠近所述锁紧件的一侧设置于所述反应器本体的外侧，所述固定件远离所述锁紧件的一侧设置于所述反应器本体内。
本技术方案中，通过固定件的设置，实现了反应器本体与护套主体之间的连接固定，减小了护套主体晃动的可能，继而减小了温度计晃动的可能，增加了温度计的稳定性。
进一步，所述固定件远离所述锁紧件一侧的护套主体上设有外密封圈，所述外密封圈的外侧用于抵接在所述反应器本体的内壁上。
本技术方案中，通过外密封圈的设置，实现护套主体与生物反应器的密封。
进一步，所述护套主体与所述锁紧件之间设有压环；所述锁紧件还用于将所述压环压紧于护套主体，并将所述第一密封圈压紧于所述压环与所述护套主体之间。
本技术方案中，通过压环的设置，压环、温度计与护套主体之间能够二次密封，进一步实现了生物反应器的密封要求。
本实用新型的目的之一还在于提供一种生物反应器，包括：反应器本体，所述反应器本体上开设有接口，所述接口上安装有一种用于生物反应器的组合型温度计。
本技术方案中，通过锁紧件与第一密封圈的设置，第一密封圈能够抵接在温度计与护套主体之间，实现了温度计与护套主体之间的密封要求；通过将温度计设置在两端开口的护套主体内，使温度计的玻璃泡端能够直接设置在生物反应器内，并与生物反应器内的液体进行直接接触，提高了温度计的检测灵敏度和准确度，从而提高了温度检测系统的标定与测量准确性。
与现有技术相比，本实用新型提供的一种用于生物反应器的组合型温度计和生物反应器具有以下有益效果：
1、温度计的玻璃泡端能够直接设置在生物反应器内，并与生物反应器内的液体进行直接接触，提高了温度计的检测灵敏度和准确度，从而提高了温度检测系统的标定与测量准确性。
2、通过压套的设置，增加了第一密封圈、第二密封圈与温度计之间的约束，压套能够对密封圈进行限位，减小了温度计晃动的可能，增加了温度计的稳定性。
3、通过第一密封圈与第二密封圈的设置，以及锁紧件与压套对密封圈进行限位，减小温度计晃动的可能，增加了温度计的稳定性；同时第二密封圈的添加，且第二密封圈抵接于温度计、压槽的内壁即护套主体上，实现了温度计与护套主体之间的第二次密封，增加了生物反应器密封的可靠性。
4、通过固定件的设置，实现了反应器本体与护套主体之间的连接固定，减小了护套主体晃动的可能，继而减小了温度计晃动的可能，增加了温度计的稳定性。
5、通过外密封圈的设置，实现了护套主体与生物反应器的密封。
附图说明
下面将以明确易懂的方式，结合附图说明优选实施方式，对一种用于生物反应器的组合型温度计和生物反应器的上述特性、技术特征、优点及其实现方式予以进一步说明。
图1是本实用新型一种用于生物反应器的组合型温度计的结构示意图。
附图标号说明：10.护套主体，11.安装孔，12.压槽，13.调节窗口，20.温度计，31.第一密封圈，32.第二密封圈，33.外密封圈，40.锁紧件，41.锁紧孔，50.压环，60.压套，70.套管，71.观察窗口，80.固定件。
具体实施方式
为了更清楚地说明本实用新型实施例或现有技术中的技术方案，下面将对 照附图说明本实用新型的具体实施方式。显而易见地，下面描述中的附图仅仅是本实用新型的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图，并获得其他的实施方式。
为使图面简洁，各图中只示意性地表示出了与实用新型相关的部分，它们并不代表其作为产品的实际结构。另外，以使图面简洁便于理解，在有些图中具有相同结构或功能的部件，仅示意性地绘示了其中的一个，或仅标出了其中的一个。在本文中，“一个”不仅表示“仅此一个”，也可以表示“多于一个”的情形。
根据本实用新型提供的一种实施例，如图1所示，一种用于生物反应器的组合型温度计，包括，护套主体10，护套主体10为长筒状，护套主体10内设有安装孔11，安装孔11沿其长度方向开设在护套主体10内，且安装孔11的两端贯穿护套主体10；安装孔11内设有温度计20，温度计20能够从护套主体10的一端穿入护套主体10内。
护套主体10的一端设有第一密封圈31，本实施例中，第一密封圈31设置在护套主体10的左端；第一密封圈31为环状，第一密封圈31的内侧抵接于温度计20，第一密封圈31的外侧抵接于护套主体10，即在护套主体10的一端通过第一密封圈31卡紧在护套主体10与温度计20之间，实现了护套主体与温度计之间的密封。
护套主体10靠近第一密封圈31的一端设有锁紧件40，即锁紧件40设置在第一密封圈31的左侧；锁紧件40上设有用于温度计20穿过的锁紧孔41，锁紧件40能够套设在护套主体10上，并将第一密封圈31压紧于护套主体10与温度计20之间，即锁紧件40能够在护套主体10的一端进行装卸，并在锁紧件40安装在护套主体10上时，锁紧件40能够将第一密封圈31压紧在护套主体10与温度计20上。在本实施例中，温度计20的玻璃泡端能够设置在护套主体10的任意一端，即温度计20的玻璃泡设置在护套主体10的左端或右端均可。
本实施例中，通过锁紧件40与第一密封圈31的设置，第一密封圈31能够抵接在温度计20与护套主体10之间，实现了护套主体10与温度计20之间的密封要求；通过将温度计20设置在两端开口的护套主体10内，即温度计20的玻璃泡端能够直接设置在反应器本体内，并与反应器本体内的液体进行直接接触，增加了温度计20的检测灵敏度和准确度，从而提高了温度检测系统的标定与测量准确性。
优选地，护套主体10与锁紧件40之间设有压环50，锁紧件40还用于将压环50压紧于护套主体10，并将第一密封圈31压紧于压环50与护套主体10之间；即锁紧件40设置在护套主体10上时，能够将压环50压向护套主体10，第一密封圈31能够压紧于压环50、护套主体10与温度计20之间。
通过压环50的设置，压环50与温度计20之间能够良好密封，进一步实现了生物反应器的密封要求。
本实施例中，由于温度计20为标准件，护套主体10靠近压环50的一侧开设有第一压紧面，压环50靠近护套主体10的一侧设有第二压紧面，第二压紧面与第一压紧面连通，第一压紧面与第二压紧面能够通过倒角形成，护套主体10、压环50与温度计20之间能形成一个用于容纳第一密封圈31的空间；优选地，第一密封圈31的截面为圆形，并在锁紧件40将压环50压紧在护套主体10上时，第一密封圈31能够同时抵接于压环50、护套主体10与温度计20之间，第一密封圈31抵接于护套主体10与温度计20之间，实现温度计20与护套主体10之间的第一次密封，第一密封圈31抵接与温度计20与压环50之间，实现温度计20与护套主体10之间的第二次密封。
本实施例中，锁紧件40能够卡接或搭扣连接或螺纹连接等任意连接方式连接在护套主体10上，优选地，锁紧件40为锁紧螺母，锁紧件40使用螺纹连接在护套主体10上，通过锁紧件40与护套主体10之间的螺纹连接，便于实现锁紧件40的锁紧与松开。
优选地，在本实施例中，当温度计20沿其轴线方向滑移设置在护套主体10内时，第一密封圈31能够始终紧密抵接在温度计20上；进一步，当锁紧件40锁紧在护套主体40上时，锁紧组件40还能够增加温度计20与护套主体10之间的约束，减小了温度计20沿其轴线方向滑动的可能，增加了温度计20在护套主体10上的稳定性。需要调节温度计20的位置时，松开锁紧件40，释放温度计20与护套主体10之间的约束，即可实现温度计20的滑移，无需将锁紧件40完全释放，操作较为方便。
根据本实用新型提供的另一种实施例，如图1所示，一种用于生物反应器的组合型温度计，本实施例与第一种实施例的区别在于第二密封圈32的添加。
在第一种实施例的基础上，本实施例中，还包括套设在温度计20上的第二密封圈32，第二密封圈32的外侧抵接于护套主体10；通过第二密封圈32的添加，实现了温度计20与护套主体10之间的第二次密封，增加了密封可靠性。具体地，护套主体10内开设用于第二密封圈32设置的压槽12，第二密封圈32的内侧能够抵接在温度计20上，第二密封圈32的外侧抵接于压槽12的内壁上。
优选的，温度计20的外侧套设有压套60，护套主体10内开设有与安装孔11连通的压槽12；压套60的一端能够抵接于第一密封圈31，压套60的另一端能够抵接于第二密封圈32。通过压套60的设置，增加了第一密封圈31、第二密封圈32与温度计20之间的约束，压套60能够对密封圈(31，32)进行限位，减小了温度计20晃动的可能，增加了温度计20的稳定性。
由于压套60的一端能够抵接在第一密封圈31上，结合第一种实施例所描述的一种用于生物反应器的组合型温度计20，第一压紧面能够开设在护套主体10上，第一密封圈31能够直接抵接于压环50、护套主体10、温度计20和压套60上，第一密封圈31能够实现温度计20与护套主体10之间密封；或直接在压套60靠近第一密封圈31的一端开设第三压紧面，第二压紧面与第三压紧面形成用于容纳第一密封圈31的空间，第一密封圈31能够直接抵接于压环50、温度计20和压套 60上，第一密封圈31能够实现温度计20与护套主体10之间的双重密封，并依据用户的需要选取合适的密封方式。
在第一种实施例的基础上，在温度计20产生滑移趋势时，第二密封圈32也能够稳定地抵接在温度计20上，同时保证了密封与对温度计20滑行的约束。
根据本实用新型提供的又一种实施例，如图1所示，一种用于生物反应器的组合型温度计，本实施例与第一种实施例的区别在于套管70的添加。
在第一种实施例的基础上，本实施例中，锁紧件40远离护套主体10的一端设有套管70，套管70内设有用于温度计20穿过的连接槽，温度计20的玻璃泡端能够设置在护套远离套管70的一端，温度计20的另一端嵌设于套管70内，即套管70能够设置在锁紧件40的左端；通过套管70的设置，对裸露在反应器本体外侧的套管70进行保护，减小了温度计20损坏的可能。
具体地，套管70能够直接一体成型，并在套管70的右侧开设连接槽；或套管70选取两端开口的管件，并在管件的左端开口处添加盖板，形成连接槽；套管70能够卡接或搭扣连接或螺纹连接等任意连接方式连接在锁紧件40上，优选地，套管70螺纹连接在锁紧件40上，便于套管70的装卸。
优选地，护套主体10远离套管70的一端设有调节窗口13，即调节窗口13设置在护套主体10的右端，调节窗口13沿护套主体10的长度方向开设在护套主体10上，温度计20的玻璃泡端能够在调节窗口13的位置，通过调节窗口13的设置，用户便于直接观察到温度计20在护套主体10内的位置，便于调节温度计20在反应器本体内的插入深度，在合适的位置检测罐体内液体温度，在保护温度计的同时，便于反应器内液体在温度计玻璃球泡端的流动，提高温度检测的灵敏度与准确性。
优选地，套管70上还设有观察窗口71，观察窗口71能够沿套管70的长度方向开设在套管70上，观察窗口71有腰型孔，用户能够通知观察窗口71直接观察到温度计20的刻度，而无需取下套管70，在保护温度计20的同时，还能够实现 温度计20的温度数值读取，便于用户的操作。
根据上述实施例的改进，本实施例中，在伸出生物反应器外侧的温度计20上还设有保温装置(图中未示出)，保温装置能设置在护套主体10或套管70上，通过保温装置的设置，可以使温度计在更符合标准温度计使用要求的条件下进行检测，进一步提高了温度计的检测准确度。
根据本实用新型提供的再一种实施例，如图1所示，一种用于生物反应器的组合型温度计，本实施例与第一种实施例的区别在于固定件80的添加。
在第一种实施例的基础上，本实施例中，护套主体10上设有用于抵接在反应器本体上的固定件80，固定件80靠近锁紧件40的一侧能够设置在反应器本体的外侧，固定件80远离锁紧件40侧一侧设置在反应器本体内，因此锁紧件40能够设置在反应器本体的外侧，同时通过固定件80的设置，实现了反应器本体与护套主体10之间的连接，减小了护套主体10晃动的可能，继而减小了温度计20晃动的可能，增加了温度计20的稳定性。
优选地，固定件80螺纹连接在护套主体10上，便于将护套主体10压紧在反应器本体上。反应器本体上开设有接口，护套主体10靠近温度计20玻璃球泡的一端能够穿过接口进入反应器本体内，固定件80能够抵接在接口外侧的反应器本体上。
固定件80远离锁紧件40的一侧设有外密封圈33，外密封圈33能够抵接在反应器的接口上，通过外密封圈33的设置，进一步增加了与生物反应器之间的密封程度，增加了生物反应器密封的可靠性。具体地，外密封圈33能够抵接在反应器本体接口的内壁上。
根据本实用新型提供的一种实施例，如图1所示，一种生物反应器，包括：
反应器本体(图中未示出)，反应器本体上开设有接口，接口上安装有上述任意一种实施例所述的用于生物反应器的组合型温度计。
应当说明的是，上述实施例均可根据需要自由组合。以上所述仅是本实用 新型的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本实用新型原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本实用新型的保护范围。

Claims (10)

  1. 一种用于生物反应器的组合型温度计，其特征在于，包括：
    护套主体，所述护套主体内开设有安装孔，所述安装孔内设有温度计；
    所述护套主体的一端还设有第一密封圈，所述第一密封圈的内侧抵接于所述温度计，所述第一密封圈的外侧抵接于所述护套主体；
    所述护套主体靠近所述第一密封圈的一端设有锁紧件，所述锁紧件上设有用于温度计穿过的锁紧孔；
    所述锁紧件用于套设在所述护套主体上，并将所述第一密封圈压紧于所述护套主体与所述温度计。
  2. 根据权利要求1所述的一种用于生物反应器的组合型温度计，其特征在于：
    还包括套设在所述温度计上的第二密封圈，所述第二密封圈的外侧抵接于所述护套主体。
  3. 根据权利要求2所述的一种用于生物反应器的组合型温度计，其特征在于：
    所述温度计的外侧套设有压套，所述护套主体内开设有与所述安装孔连通的压槽；
    所述压套的一端抵接于所述第一密封圈，所述压套的另一端抵接于所述第二密封圈。
  4. 根据权利要求1所述的一种用于生物反应器的组合型温度计，其特征在于：
    所述锁紧件远离所述护套主体的一端还设有套管，所述套管内设有用于温度计穿过的连接槽；
    所述温度计的玻璃泡端设置在所述护套主体远离所述套管的一端，所述温度计的另一端嵌设于所述套管内。
  5. 根据权利要求4所述的一种用于生物反应器的组合型温度计，其特征在于：
    所述护套主体远离所述套管的一端设有调节窗口，所述调节窗口沿所述护套主体的长度方向开设于所述护套主体。
  6. 根据权利要求4所述的一种用于生物反应器的组合型温度计，其特征在于：
    所述套管上还开设有观察窗口，所述观察窗口沿所述套管的长度方向开设于所述套管。
  7. 根据权利要求1所述的一种用于生物反应器的组合型温度计，其特征在于：
    所述护套主体上套设有用于抵接在反应器本体上的固定件，所述固定件靠近所述锁紧件的一侧设置于所述反应器本体的外侧，所述固定件远离所述锁紧件的一侧设置于所述反应器本体内。
  8. 根据权利要求7所述的一种用于生物反应器的组合型温度计，其特征在于：
    所述固定件远离所述锁紧件一侧的护套主体上设有外密封圈，所述外密封圈的外侧用于抵接在所述反应器本体的内壁上。
  9. 根据权利要求1～8中任意一项所述的一种用于生物反应器的组合型温度计，其特征在于：
    所述护套主体与所述锁紧件之间设有压环；
    所述锁紧件还用于将所述压环压紧于护套主体，并将所述第一密封圈压紧于所述压环与所述护套主体之间。
  10. 一种生物反应器，其特征在于，包括：
    反应器本体，所述反应器本体上开设有接口，所述接口上安装有权利要求1～9中任意一项所述的一种用于生物反应器的组合型温度计。
PCT/CN2020/072882 2019-01-24 2020-01-17 一种用于生物反应器的组合型温度计和生物反应器 Ceased WO2020151602A1 (zh)

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CN209513074U (zh) * 2019-01-24 2019-10-18 华东理工大学青岛创新研究院 一种用于生物反应器的组合型温度计和生物反应器

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