WO2020139260A1 - An algae removal method by using bacteria culture - Google Patents
An algae removal method by using bacteria culture Download PDFInfo
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- WO2020139260A1 WO2020139260A1 PCT/TR2019/051118 TR2019051118W WO2020139260A1 WO 2020139260 A1 WO2020139260 A1 WO 2020139260A1 TR 2019051118 W TR2019051118 W TR 2019051118W WO 2020139260 A1 WO2020139260 A1 WO 2020139260A1
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- WO
- WIPO (PCT)
- Prior art keywords
- algae
- bacteria
- algae removal
- removal method
- medium
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/42—Nature of the water, waste water, sewage or sludge to be treated from bathing facilities, e.g. swimming pools
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/20—Prevention of biofouling
Definitions
- the invention is particularly relater to algae removal method in which bacteria culture is used in order to remove the algae which is formed in thermal areas, thermal water and mud pools.
- the main aim of the invention is to provide an algae removal method which enables eliminating the algae formed in the thermal pools without damaging the human health.
- An aim of the invention is to provide a method in which the algae are removed without forming any toxic side products by means of a totally biological method.
- Another aim of the invention is to provide a method in which the algae are removed without changing the quality of the water and mud which is cleaned.
- Another aim of the invention is to provide a method in which the algae are removed which makes a great contribution to the national economy by means of increasing the preferability of the thermal areas.
- the invention is related to an algae removal method for eliminating the algae pollution formed within the thermal water and mud. Accordingly, said algae removal method comprises the following process steps:
- the bacteria cultures in step (c) are preferably Lactobacillus paracasei subsp. tolerance and Anoxbacillus thermarum bacteria.
- said bacteria culture are added in amounts of 1 ml. in each conical flask.
- the incubation process in said step (d) is preferably performed with pH 5, 5/5, 5 at S /SSO'de during 5 days.
- the effect of Lactobacillus paracasei subsp. tolerance and Anoxbacillus thermarum bacteria on the removal of the algae and blurriness is determined by means of observing the reaction medium during preferably 5 days at different pH degrees.
- said Lactobacillus paracasei subsp. Tolerance bacteria realizes the most algae removal at pH 5.5 and 37 ⁇ .
- said Anoxbacillus thermarum bacteria realizes the most algae removal at pH 5.5 and 55TT
- Figure 1 is a general view of the inventive process flow of the algae removal method by using bacteria culture.
- the invention is related to a method in which the algae accumulated in time in environments such as thermal field, thermal water and mud and mud pools etc. are eliminated by means of the inoculated bacteria culture in the medium where algae exist.
- a general process flow in terms of the method is given in Figure 1.
- the algae removal method comprises an experimental method particularly as an algae removal method, then inoculation of the bacteria cultures with determined values by considering the results of this experiment into the medium where the algae is required to be removed.
- the thermal water, mud and algae provided from the environment including algae is primarily mixed in a homogenous manner. Subsequently these obtained homogenous mixtures are divided into two groups in 6 conical flasks.
- the Lactobacillus paracasei subsp. tolerance and Anoxbacillus thermarum bacteria whose activity are determined are taken from the stock cultures and revived within appropriate nutritive medium and inoculated into reaction mediums where algae pollution exists. Therefore the bacteria culture removes the algae pollution.
- each reaction medium is left to incubation during 5 days within an agitating incubator by means of adjusting the medium to be pH 5 and 25TT
- the reactions maintained in the conical flasks are kept during 5 days in environments with preferably 25 - 35 ⁇ by means of taking measurement s at one day intervals.
- control group is provided by means of creating reaction medium by means of adding distilled water instead of bacteria culture into the experiment medium within 3 different conical flasks. Then the experiment results are examined in a spectrophotometric manner by accepting the control group as blank. Following this, the experiment results are photographed. Microbial developments of the bacteria are examined in a spectrophotometric and visual manner.
- the algae removal is provided wherein 0.4 % and preferably 0.37 % bacteria culture by weight is added into a thermal water which includes 8 % and preferably 7,41 % algae by weight.
- the algae are removed without forming any toxic side products by means of using bacteria cultures with biological characteristic. Also algae removal is enabled without damaging the human health and without changing the quality of the water and mud which is cleaned.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention is related to an algae removal method in which a reaction medium obtained by means of adding preferably Lactobacillus paracasei subsp. tolerance and Anoxbacillus thermarum bacteria into the experimental medium which is homogenized with the mixture of thermal water, mud and algae is evaluated in a spectrophotometric and visual manner by means of the control group formed by adding distilled water into the experiment medium and as a result of these evaluations, after the bacteria culture is revived within a nutritive medium and their purity is determined, they are inoculated into the medium where there is algae pollution.
Description
AN ALGAE REMOVAL METHOD BY USING BACTERIA CULTURE
Technical Field
The invention is related to a method which provides algae removal by using bacteria culture.
The invention is particularly relater to algae removal method in which bacteria culture is used in order to remove the algae which is formed in thermal areas, thermal water and mud pools.
State of the Art
Today the treatments in which applications such as thermo mineral water bath, respiration, mineral springs, mud bath etc. and the treatments in which these applications are combined with other treatments are included within the thermal tourism subject which is a sub branch of the health tourism. Thermal treatments are provided by means of hot and cold waters provided from thermal sources in the thermal fields (regions) including some melts and mines within it. It is very widespread to treat the rheumatic diseases and arthralgia etc. disorders in the thermal fields. In addition to this, in case thermal sources are utilized as a bath; it is known that it is beneficial for anemia, hypertension, rheumatoid, skin diseases, metabolic disorders, neuralgias, neuritis, nutrition disorders and when it is drunk it is known that it is beneficial for peptic and it has a treating effect for the stomach. For these reasons thermal areas are very important in terms of health tourism and they make a great contribution to the national economy.
Thermal treatments are mostly applied in the pools where thermal water or mud is filled. The person waits during a determined period within these pools according to the treatment to be applied. In said pools algae can be formed in time and this algae accumulation creates an unhealthy environment and bothers people for its usage both in terms of health and in terms of visual pollution. Algae removal processes is provided by means of removing the algae via a tool such as a shovel etc. in a physical manner in standard applications. Another alternative cleaning process is performed by means of
using chemicals. In this manner the following components are added into the pools and the algae is removed; AI2(SC>4)3, CaCC>3, MgCC>3, FeC and NaOH etc.
In these developed physical and chemical applications, thermal water or mud cannot be totally cleaned from the algae. Since the algae cannot be removed totally, the water becomes blurred and it creates risk in terms of health and also it leads to undesired situations in terms of its appearance. In addition to these applied chemical applications leads to a decrease in the quality of the thermal water and mud and also the chemical substances which creates risk in terms of human health can cause serious health problems after they are accumulated within the environment.
As a result, due to the above mentioned disadvantages and the insufficiency of the current solutions in terms of the issuer, making a development in the relevant technical field is required.
Brief Description of the Invention
The present invention is related to an algae removal method by means of bacteria culture usage in order to eliminate the algae accumulation in thermal water and mud pools which fulfills the abovementioned requirements, eliminates all disadvantages and brings some additional advantages.
The main aim of the invention is to provide an algae removal method which enables eliminating the algae formed in the thermal pools without damaging the human health.
An aim of the invention is to provide a method in which the algae are removed without forming any toxic side products by means of a totally biological method.
Another aim of the invention is to provide a method in which the algae are removed without changing the quality of the water and mud which is cleaned.
Another aim of the invention is to provide a method in which the algae are removed which makes a great contribution to the national economy by means of increasing the preferability of the thermal areas.
In order to fulfill the above mentioned aims, the invention is related to an algae removal method for eliminating the algae pollution formed within the thermal water and mud. Accordingly, said algae removal method comprises the following process steps:
a) Homogenizing said thermal water, mud and algae after mixing thereof,
b) Dividing the homogenized mixture within the conical flasks,
c) Reviving the bacteria whose activity is determined within a nutritive medium by means of taking thereof from the stock culture of the bacteria,
d) Inoculating the bacteria into a medium in which algae pollution exists after an amount is taken from the bacteria culture,
e) Incubating each reaction medium within the agitating incubator,
f) Forming the control group by means of adding distilled water within the experimental medium obtained from the homogenized mixture,
g) Examining the experimental group and the control group visually in a spectrophotometric manner by accepting the formed control group as blank.
In order to realize the aims of the invention, the division of the homogenized mixture in step (b) is performed in preferably in 6 conical flasks in 2 groups.
In order to realize the aims of the invention, the bacteria cultures in step (c) are preferably Lactobacillus paracasei subsp. tolerance and Anoxbacillus thermarum bacteria.
In order to realize the aims of the invention, said bacteria culture are added in amounts of 1 ml. in each conical flask.
In order to realize the aims of the invention, the incubation process in said step (d) is preferably performed with pH 5, 5/5, 5 at S /SSO'de during 5 days.
In order to realize the aims of the invention, said control group is preferably arranged in 3 repetitions.
In order to realize the aims of the invention, the effect of Lactobacillus paracasei subsp. tolerance and Anoxbacillus thermarum bacteria on the removal of the algae and blurriness is determined by means of observing the reaction medium during preferably 5 days at different pH degrees.
In order to realize the aims of the invention, said Lactobacillus paracasei subsp. Tolerance bacteria realizes the most algae removal at pH 5.5 and 37Ό.
In order to realize the aims of the invention, said Anoxbacillus thermarum bacteria realizes the most algae removal at pH 5.5 and 55TT
In order to realize the aims of the invention, the algae removal is provided wherein 0.4 % and preferably 0.37 % bacteria culture by weight is added into a thermal water which includes 8 % and preferably 7,41 % algae by weight.
The structural and characteristic features of the present invention will be understood clearly by the following drawings and the detailed description made with reference to these drawings and therefore the evaluation shall be made by taking these figures and the detailed description into consideration.
Figures Clarifying the Invention
Figure 1 , is a general view of the inventive process flow of the algae removal method by using bacteria culture.
The drawings are not required to be scaled and the details which are not necessary for clarifying the present invention can be omitted. Apart from these, the elements which are substantially identical or at least which have substantially identical functions are shown by the same numbers.
Detailed Description of the Invention
In this detailed description the inventive algae removal method by using bacteria culture is described only for clarifying the subject matter in a manner such that no limiting effect is created.
The invention is related to a method in which the algae accumulated in time in environments such as thermal field, thermal water and mud and mud pools etc. are eliminated by means of the inoculated bacteria culture in the medium where algae exist. A general process flow in terms of the method is given in Figure 1. Accordingly, the algae removal method comprises an experimental method particularly as an algae removal
method, then inoculation of the bacteria cultures with determined values by considering the results of this experiment into the medium where the algae is required to be removed.
In the algae removal method, the thermal water, mud and algae provided from the environment including algae is primarily mixed in a homogenous manner. Subsequently these obtained homogenous mixtures are divided into two groups in 6 conical flasks. The Lactobacillus paracasei subsp. tolerance and Anoxbacillus thermarum bacteria whose activity are determined are taken from the stock cultures and revived within appropriate nutritive medium and inoculated into reaction mediums where algae pollution exists. Therefore the bacteria culture removes the algae pollution.
1 ml. is added from the bacteria samples developed in each conical flask. Here preferably Lactobacillus paracasei subsp. tolerance and Anoxbacillus thermarum bacteria are used which have a wide usage potential in food and different industrial fields. Following the addition of the bacteria culture, each reaction medium is left to incubation during 5 days within an agitating incubator by means of adjusting the medium to be pH 5 and 25TT The reactions maintained in the conical flasks are kept during 5 days in environments with preferably 25 - 35Ό by means of taking measurement s at one day intervals.
In order to compare the experimental group, control group is provided by means of creating reaction medium by means of adding distilled water instead of bacteria culture into the experiment medium within 3 different conical flasks. Then the experiment results are examined in a spectrophotometric manner by accepting the control group as blank. Following this, the experiment results are photographed. Microbial developments of the bacteria are examined in a spectrophotometric and visual manner.
Determining the effect of the bacteria culture used in terms of the method on the removal of the algae and blurriness;
The effect of the Lactobacillus paracasei subsp. tolerance bacteria; the reaction medium is observed during 5 days at seven different pH (4, 4.5, 5, 5.5, 6, 7 and 8) in order to understand the effect of the Lactobacillus paracasei subsp. tolerance bacteria on the removal of the algae and blurriness. Bacteria culture is added to each sample, and the most algae removal is observed to be realized at pH 5.5 and 37Ό among these results. These results are seen in the following Graph 1.
Graph 1. The effect of pH and time on the algae removal with the Lactobacillus paracasei bacteria
The effect of the Anoxbacillus thermarum bacteria; the reaction medium is observed during 5 days at seven different pH (4, 4.5, 5, 5.5, 6, 7 and 8) in order to understand the effect of the Anoxbacillus thermarum bacteria on the removal of the algae and blurriness. Bacteria culture is added to each sample, and the most algae removal is observed to be realized at pH 5.5 and 55Ό among these results. Th ese results are seen in the following Graph 2.
Graph 2. The effect of pH and time on the algae removal with the Anoxbacillus thermarum bacteria
Determining the algae amount on which the bacteria used for the method have maximum effect during algae removal; algae between 3-9 gr algae is used and it is determined that maximum 7.41 g algae is removed from the environment. The obtained results show that the removal of maximum 8 % algae level by weight within the reaction environment is enabled. During this evaluation phase, the table in terms of the obtained values is given in the following table.
Lactobacillus paracasei subsp. tolerance bacteria have maximum effect during algae removal
In terms of these evaluations and applied method, the algae removal is provided wherein 0.4 % and preferably 0.37 % bacteria culture by weight is added into a thermal water which includes 8 % and preferably 7,41 % algae by weight. Together with the inventive method, the algae are removed without forming any toxic side products by means of using bacteria cultures with biological characteristic. Also algae removal is enabled without damaging the human health and without changing the quality of the water and mud which is cleaned.
Claims
1. An algae removal method in order to eliminate the algae pollution formed in the thermal water and mud, characterized by comprising the following process steps;
a) Homogenizing said thermal water, mud and algae after mixing thereof, b) Dividing the homogenized mixture within the conical flasks,
c) Reviving the bacteria whose activity is determined within a nutritive medium by means of taking thereof from the stock culture of the bacteria,
d) Inoculating the bacteria into a medium in which algae pollution exists after an amount is taken from the bacteria culture,
e) Incubating each reaction medium within the agitating incubator,
f) Forming the control group by means of adding distilled water within the experimental medium obtained from the homogenized mixture,
g) Examining the experimental group and the control group visually in a spectrophotometric manner by accepting the formed control group as blank.
2. The algae removal method according to claim 1 , characterized in that; the division of the homogenized mixture in step (b) is performed in preferably in 6 conical flasks in 2 groups.
3. The algae removal method according to claim 1 , characterized in that; said bacteria cultures in step (c) are preferably Lactobacillus paracasei subsp. tolerance and Anoxbacillus thermarum bacteria.
4. The algae removal method according to claim 1 and 3, characterized in that; said bacteria culture are added in amounts of 1 mL in each conical flask.
5. The algae removal method according to claim 1 , characterized in that; the incubation process in said step (e) is preferably performed with pH 5, 5/5, 5 at 37/55T! b during 5 days.
6. The algae removal method according to claim 1 , characterized in that; said control group is preferably arranged in 3 repetitions.
7. The algae removal method according to claim 1 and 3, characterized in that; the effect of Lactobacillus paracasei subsp. tolerance and Anoxbacillus thermarum bacteria on
the removal of the algae and blurriness is determined by means of observing the reaction medium during preferably 5 days at different pH degrees.
8. The algae removal method according to claim 7, characterized in that; said Lactobacillus paracasei subsp. Tolerance bacteria realizes the most algae removal at pH 5.5 and 37TT
9. The algae removal method according to claim 1 , characterized in that; said Anoxbacillus thermarum bacteria realizes the most algae removal at pH 5.5 and 55TT
10. The algae removal method according to any of the preceding claims, characterized in that; the algae removal is provided wherein 0.4 % and preferably 0.37 % bacteria culture by weight is added into a thermal water which includes 8 % and preferably 7,41 % algae by weight.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TR2018/20942A TR201820942A2 (en) | 2018-12-28 | 2018-12-28 | MOSSING METHOD BY USING BACTERIA CULTURE |
| TR2018/20942 | 2018-12-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2020139260A1 true WO2020139260A1 (en) | 2020-07-02 |
Family
ID=67955830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/TR2019/051118 WO2020139260A1 (en) | 2018-12-28 | 2019-12-19 | An algae removal method by using bacteria culture |
Country Status (2)
| Country | Link |
|---|---|
| TR (1) | TR201820942A2 (en) |
| WO (1) | WO2020139260A1 (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015007419A1 (en) * | 2013-07-15 | 2015-01-22 | Direvo Industrial Biotechnology Gmbh | Methods for producing carbon-based chemicals by algal biomass processing |
| US20170121198A1 (en) * | 2015-11-02 | 2017-05-04 | BiOWiSH Technologies, Inc. | Compositions and methods of use for reducing evaporative loss from swimming pools and spas |
-
2018
- 2018-12-28 TR TR2018/20942A patent/TR201820942A2/en unknown
-
2019
- 2019-12-19 WO PCT/TR2019/051118 patent/WO2020139260A1/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015007419A1 (en) * | 2013-07-15 | 2015-01-22 | Direvo Industrial Biotechnology Gmbh | Methods for producing carbon-based chemicals by algal biomass processing |
| US20170121198A1 (en) * | 2015-11-02 | 2017-05-04 | BiOWiSH Technologies, Inc. | Compositions and methods of use for reducing evaporative loss from swimming pools and spas |
Non-Patent Citations (1)
| Title |
|---|
| GOH K.M. ET AL.: "Recent discoveries and applications of Anoxybacillus", APPL MICROBIOL BIOTECHNOL, vol. 97, 17 January 2013 (2013-01-17), pages 1475 - 1488, XP035328863, DOI: 10.1007/s00253-012-4663-2 * |
Also Published As
| Publication number | Publication date |
|---|---|
| TR201820942A2 (en) | 2019-02-21 |
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