WO2020100981A1 - Highly dispersible ceramide composition - Google Patents
Highly dispersible ceramide composition Download PDFInfo
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- WO2020100981A1 WO2020100981A1 PCT/JP2019/044702 JP2019044702W WO2020100981A1 WO 2020100981 A1 WO2020100981 A1 WO 2020100981A1 JP 2019044702 W JP2019044702 W JP 2019044702W WO 2020100981 A1 WO2020100981 A1 WO 2020100981A1
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- glycosphingolipid
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- the present invention relates to a ceramide composition having excellent dispersibility in water and a method for producing the same.
- Ceramide is a kind of glycosphingolipid, and is a general term for a compound group in which sphingosine, which is a long-chain amino alcohol, and a fatty acid are amide-bonded. It is known that this ceramide is present in a high concentration in the cell membrane, and in particular, it exists as a main component of the interkeratinocyte lipid of the skin, and has a barrier function as a moisturizing ability to prevent the evaporation of water from the inside of the skin. I carry it. However, it has been reported that ceramide in inter-keratinocyte lipids decreases with age and the moisturizing ability declines, which causes dry skin, wrinkles, or rough skin.
- the ceramide content in the skin is one of the indicators of the health condition of the skin, and it is said that supplementation of the insufficient ceramide is effective for the moisturizing effect on the skin or the skin-beautifying effect, so that the efficiency is high. It can be said that demand for ceramide as a functional food or as a raw material for cosmetics will increase in the future.
- the present inventors also paid attention to useful trace components contained in by-products produced in the process of producing vegetable rice oil, particularly rice bran as a raw material, and conducted a study, as a result, a method for extracting and producing rice ceramide.
- various studies have been conducted on various ceramide components derived from plants such as various fruits and vegetables (Patent Document 1).
- peach is said to have a high content of ceramide, and the present inventors have proposed a technique for efficiently extracting ceramide (peach ceramide: glycosphingolipid) from peach (Patent Document 2). ).
- Ceramide glycosphingolipid
- Ceramide is an amphoteric substance having a hydrophilic sugar, a lipophilic lipid and a sphingoid in the molecule, but its dispersibility in water is not preferable. Therefore, in order to obtain a uniform aqueous suspension of glycosphingolipid, it is necessary to add a large amount of a chemically synthesized surfactant, which is unfavorable to the living body, in order to obtain a uniform dispersibility in water. It was
- Japanese Patent No. 4108069 Publication Japanese Patent No. 5986160 JP, 2016-124798, A JP, 2008-014966, A
- the present invention is for a uniform and stable aqueous suspension of ceramide (glycosphingolipid) without adding a chemically synthesized surfactant and only with a component having a skin beautiful effect.
- An object is to provide a composition and a method for producing the composition.
- FIG. 1 is a diagram showing the evaluation of dispersibility of compositions having different mixing ratios of collagen peptide and glycosphingolipid in Example 4.
- such plant material-derived glycosphingolipid for example, rice bran, rice germ and / or glycosphingolipid derived therefrom, more preferably, the glycosphingolipid is as ⁇ -glucosylceramide ( ⁇ -GlcCer).
- ⁇ -GlcCer ⁇ -glucosylceramide
- the glycosphingolipid (come ceramide) consisting of the purified glycosphingolipid fraction obtained by Further, a glycosphingolipid (peach ceramide) obtained from peach prepared according to Patent Document 2 can be mentioned.
- a composition prepared by mixing a collagen peptide with a specific ratio of glycosphingolipid is a stable suspension solution when the glycosphingolipid is uniformly dispersed in water. It was confirmed.
- the collagen peptide to be mixed with the glycosphingolipid is not particularly limited in its material.
- commercially available “Nippi peptide PRA” Nippi Corporation
- “water-soluble collagen peptide SS” Kyowa Hakko Bio Co., Ltd.
- Collagen peptide SCP (Nitta Gelatin Co., Ltd.)
- HACP Zeraisu Co., Ltd.)
- the content of glycosphingolipid in the composition is preferably 5 to 50% by weight based on the total weight of the composition. Therefore, the content of the glycosphingolipid is selected according to the intended use of the composition, and the amount of the collagen peptide based on the specific ratio of the present invention is selected accordingly, and both are homogeneously mixed in the solvent. Thereby, the composition of the present invention is prepared.
- both the glycosphingolipid and the collagen peptide are homogeneously mixed and produced in a solvent
- a solvent examples include water, a lower alcohol, a polyhydric alcohol, or acetone. Alternatively, a mixture of two or more of these can be used.
- the composition provided by the present invention requires ingestion of glycosphingolipids orally, or from the viewpoint of use as a raw material for cosmetics, it is necessary to use a non-toxic solvent for mixing, and therefore, as a solvent. Is preferably water and / or ethanol. It is possible to use other solvents for the mixing, but it is not preferable because there is a problem of residual solvent which is concerned about safety.
- the composition of the present invention is prepared by pulverizing and homogenizing the residue using a mortar, and then performing a vacuum drying treatment (eg, 80 ° C., ⁇ 0.1 MPa) to completely distill off the solvent. To do.
- a vacuum drying treatment eg, 80 ° C., ⁇ 0.1 MPa
- Example 2 Method for preparing composition of the present invention by method B 2 g of rice-derived glycosphingolipid (manufactured by Okayasu Co., Ltd.) (HPLC content 81.8%) was mixed with 10 mL of 95% ethanol (5 volumes), and then added on a water bath. While heating to 50 ° C., ultrasonic treatment was performed to completely dissolve it. Separately, purified water (65 mL, 5 times amount) was added to 13 g of fish-derived collagen peptide (Nippi peptide: manufactured by Nippi Co., Ltd.), and ultrasonic treatment was performed while heating to 50 ° C. on a water bath to dissolve.
- Example 3 Preparation of a composition of the present invention in which the ceramide content was changed Even for a composition having a glycosphingolipid content ratio of 1, 5, 25, 50, 75%, the compounding ratio of ceramide and collagen peptide was changed.
- the target composition was prepared by the same method (both methods A and B).
- Example 4 Evaluation of dispersibility of composition The dispersibility of the composition obtained in each of the above-described examples in water was evaluated.
- ⁇ Method> Purified water is added to the test substance so that the test substance is 7.50% (w / v) in a bottle with a lid. The sample is shaken vigorously for about 1 minute to mix. The sample after mixing is quickly transferred to a measuring cell, and the transmittance at a wavelength of 660 nm is measured by an ultraviolet-visible spectrophotometer after standing. The measurement time was set to 0, 1, 3, 5, 10, 20, 30 minutes after standing, and the dispersibility was evaluated by the change in the transmittance.
- the glycosphingolipid concentration that maintains preferable uniform dispersibility of the suspension is 5 to 75% (permeability after 10 minutes of standing: 5% or less, 20 Transmittance after 10 minutes: 10% or less, Transmittance after 30 minutes: 10% or less). More preferably, the glycosphingolipid concentration was 10 to 25% (permeability after 10 to 30 minutes of standing: 1% or less).
- Example 5 Comparative Study of Permeability As described above, regarding the dispersibility of the composition containing the glycosphingolipid in water, the lower the permeability, the better the uniformity as a solution. The dispersibility of the solution is maintained. Therefore, for the mixture of glycosphingolipid and peptide collagen, the permeability was compared between the composition prepared according to the present invention and a simple mixed composition of both (simple mixture). The results are shown in Table 2 below.
- composition provided by the present invention may further contain additional components as long as it does not adversely affect the intended purpose.
- additional components include, for example, vitamins, minerals, nutritional ingredients, flavors, antioxidants, pigments, preservatives, seasonings, sweeteners, acidulants, pH adjusters, stabilizers, tonicity agents, Mention may be made of emulsifiers, buffers, nutrient enhancers and preservatives.
- the composition provided by the present invention can be orally taken as a food or drink, and can be provided as a raw material for cosmetics.
- a health food such as a functionally labeled food, a food for specified health use (Tokuho), a supplement, a drink (including a solution and a suspension). It may be provided in the form of a soft drink, a tea drink, a lactic acid bacterium drink, or the like, but is not limited thereto.
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Abstract
The objective of the present invention is to provide a composition for uniform aqueous suspension having ceramide (sphingoglycolipid), and a method for producing same, wherein a chemically synthesized surfactant is not added. This sphingoglycolipid composition contains both sphingoglycolipid and collagen peptide at a weight ratio of collagen peptide:sphingoglycolipid=99:1 to 25:75, and has excellent dispersibility into water. This method for producing a sphingoglycolipid composition having excellent dispersibility into water is characterized by comprising mixing, in a solvent, both sphingoglycolipid and collagen peptide at a weight ratio of collagen peptide:sphingoglycolipid=99:1 to 25:75, and making the resultant mixture have a dry powder form.
Description
本発明は、水中における分散性に優れたセラミド組成物及びその製造方法に関する。
The present invention relates to a ceramide composition having excellent dispersibility in water and a method for producing the same.
セラミドは、スフィンゴ糖脂質の一種であり、長鎖アミノアルコールであるスフィンゴシンと脂肪酸とがアミド結合した化合物群の総称である。このセラミドは、細胞膜に高濃度で存在することが知られており、特に、皮膚の角質細胞間脂質の主成分として存在し、皮膚内部からの水分の蒸発を防止する保湿能としてのバリア機能を担っている。
しかしながら、加齢と共に角質細胞間脂質中のセラミドが減少し、保湿能が衰退することから、乾燥肌、シワ、或いは肌荒れの原因となることが報告されている。 Ceramide is a kind of glycosphingolipid, and is a general term for a compound group in which sphingosine, which is a long-chain amino alcohol, and a fatty acid are amide-bonded. It is known that this ceramide is present in a high concentration in the cell membrane, and in particular, it exists as a main component of the interkeratinocyte lipid of the skin, and has a barrier function as a moisturizing ability to prevent the evaporation of water from the inside of the skin. I carry it.
However, it has been reported that ceramide in inter-keratinocyte lipids decreases with age and the moisturizing ability declines, which causes dry skin, wrinkles, or rough skin.
しかしながら、加齢と共に角質細胞間脂質中のセラミドが減少し、保湿能が衰退することから、乾燥肌、シワ、或いは肌荒れの原因となることが報告されている。 Ceramide is a kind of glycosphingolipid, and is a general term for a compound group in which sphingosine, which is a long-chain amino alcohol, and a fatty acid are amide-bonded. It is known that this ceramide is present in a high concentration in the cell membrane, and in particular, it exists as a main component of the interkeratinocyte lipid of the skin, and has a barrier function as a moisturizing ability to prevent the evaporation of water from the inside of the skin. I carry it.
However, it has been reported that ceramide in inter-keratinocyte lipids decreases with age and the moisturizing ability declines, which causes dry skin, wrinkles, or rough skin.
また、皮膚の老化に限らず、アトピー性皮膚炎によって起こる肌のカサつき、肌荒れなどの要因として、セラミドが不足していることが報告されている。すなわち、アトピー性皮膚炎における乾燥肌は、もともとセラミドが少ないという特徴があり、皮膚のバリア機能の低下に伴い、皮膚内部から外部へ水分が失われやすくなり、皮膚がカサカサと乾燥して、抗原や化学物質が皮膚内部に入りやすく、その結果アレルギー反応が起こり、かゆみや炎症などの様々な症状を呈することが報告されている。
Also, it is reported that ceramide is deficient not only due to aging of the skin but also as factors such as dryness and rough skin caused by atopic dermatitis. That is, dry skin in atopic dermatitis originally has a characteristic that the amount of ceramide is low, and with a decrease in the barrier function of the skin, water is easily lost from the inside of the skin to the outside, the skin becomes dry and dry, and the antigen It has been reported that chemical substances and chemical substances easily enter the skin, resulting in an allergic reaction and various symptoms such as itch and inflammation.
このように、皮膚におけるセラミド含量は、皮膚の健康状態の一つの指標とされており、不足したセラミドの補充が肌の保湿効果、或いは美肌効果に有効であるとされていることから、効率よくセラミドを摂取する機能性食品として、或いは化粧品原料として、今後その需要が益々高まるものといえる。
As described above, the ceramide content in the skin is one of the indicators of the health condition of the skin, and it is said that supplementation of the insufficient ceramide is effective for the moisturizing effect on the skin or the skin-beautifying effect, so that the efficiency is high. It can be said that demand for ceramide as a functional food or as a raw material for cosmetics will increase in the future.
本発明者等も植物油脂、特に米の糠を原料とした食用こめ油の製造過程で生成される副産物に含まれる有用微量成分に着目し、検討を行い、その結果、コメセラミドの抽出製造法を確立し、天然由来のコメセラミド（スフィンゴ糖脂質）を提供してきている。
また、最近に至り、種々の果実、或いは野菜等、植物由来のセラミド成分の研究が種々検討されてきている（特許文献１）。そのなかでも、桃にはセラミドの含有量が多いとされており、本発明者らは、桃からセラミド（モモセラミド：スフィンゴ糖脂質）を効率よく抽出する技術を提案してきている（特許文献２）。 The present inventors also paid attention to useful trace components contained in by-products produced in the process of producing vegetable rice oil, particularly rice bran as a raw material, and conducted a study, as a result, a method for extracting and producing rice ceramide. Has been established to provide naturally-occurring rice ceramide (glycosphingolipid).
Further, recently, various studies have been conducted on various ceramide components derived from plants such as various fruits and vegetables (Patent Document 1). Among them, peach is said to have a high content of ceramide, and the present inventors have proposed a technique for efficiently extracting ceramide (peach ceramide: glycosphingolipid) from peach (Patent Document 2). ).
また、最近に至り、種々の果実、或いは野菜等、植物由来のセラミド成分の研究が種々検討されてきている（特許文献１）。そのなかでも、桃にはセラミドの含有量が多いとされており、本発明者らは、桃からセラミド（モモセラミド：スフィンゴ糖脂質）を効率よく抽出する技術を提案してきている（特許文献２）。 The present inventors also paid attention to useful trace components contained in by-products produced in the process of producing vegetable rice oil, particularly rice bran as a raw material, and conducted a study, as a result, a method for extracting and producing rice ceramide. Has been established to provide naturally-occurring rice ceramide (glycosphingolipid).
Further, recently, various studies have been conducted on various ceramide components derived from plants such as various fruits and vegetables (Patent Document 1). Among them, peach is said to have a high content of ceramide, and the present inventors have proposed a technique for efficiently extracting ceramide (peach ceramide: glycosphingolipid) from peach (Patent Document 2). ).
保湿効果を目的としてセラミドを補充するためには、経口的に摂取するか、或いは化粧品として肌に適用する場合には、セラミド自体を水溶性懸濁液として適用することが考えられるが、植物由来のセラミド（スフィンゴ糖脂質）は、分子内に親水性の糖と親油性の脂質とスフィンゴイドを持つ両性物質であるが、水に対する分散性が好ましいものではない。
したがって、スフィンゴ糖脂質の均一な水懸濁液を得るには、水中への均質な分散性を得るために、生体にとって好ましくない多量の化学的に合成された界面活性剤を添加する必要があった。 To supplement ceramide for the purpose of moisturizing effect, it may be taken orally or, when applied to the skin as a cosmetic, ceramide itself may be applied as an aqueous suspension. Ceramide (glycosphingolipid) is an amphoteric substance having a hydrophilic sugar, a lipophilic lipid and a sphingoid in the molecule, but its dispersibility in water is not preferable.
Therefore, in order to obtain a uniform aqueous suspension of glycosphingolipid, it is necessary to add a large amount of a chemically synthesized surfactant, which is unfavorable to the living body, in order to obtain a uniform dispersibility in water. It was
したがって、スフィンゴ糖脂質の均一な水懸濁液を得るには、水中への均質な分散性を得るために、生体にとって好ましくない多量の化学的に合成された界面活性剤を添加する必要があった。 To supplement ceramide for the purpose of moisturizing effect, it may be taken orally or, when applied to the skin as a cosmetic, ceramide itself may be applied as an aqueous suspension. Ceramide (glycosphingolipid) is an amphoteric substance having a hydrophilic sugar, a lipophilic lipid and a sphingoid in the molecule, but its dispersibility in water is not preferable.
Therefore, in order to obtain a uniform aqueous suspension of glycosphingolipid, it is necessary to add a large amount of a chemically synthesized surfactant, which is unfavorable to the living body, in order to obtain a uniform dispersibility in water. It was
しかしながら、セラミド懸濁液を飲食品として経口的に摂取する場合、或いは敏感な肌に適用する化粧品原料として使用する場合には、化学的に合成された界面活性剤の使用を極力回避することが好ましい。
そのため、これまでにセラミドを含め、コラーゲン、或いはヒアルロン酸等を含有する組成物の懸濁水溶液を得る技術が種々提案されているが（例えば、特許文献３、特許文献４等）、いまだに満足するものが得られていないのが現状である。 However, when the ceramide suspension is orally ingested as a food or drink, or when it is used as a cosmetic raw material applied to sensitive skin, it is possible to avoid the use of a chemically synthesized surfactant as much as possible. preferable.
Therefore, various techniques for obtaining a suspension aqueous solution of a composition containing collagen, hyaluronic acid, or the like including ceramide have been proposed so far (for example, Patent Documents 3 and 4), but still satisfactory. The current situation is that nothing has been obtained.
そのため、これまでにセラミドを含め、コラーゲン、或いはヒアルロン酸等を含有する組成物の懸濁水溶液を得る技術が種々提案されているが（例えば、特許文献３、特許文献４等）、いまだに満足するものが得られていないのが現状である。 However, when the ceramide suspension is orally ingested as a food or drink, or when it is used as a cosmetic raw material applied to sensitive skin, it is possible to avoid the use of a chemically synthesized surfactant as much as possible. preferable.
Therefore, various techniques for obtaining a suspension aqueous solution of a composition containing collagen, hyaluronic acid, or the like including ceramide have been proposed so far (for example, Patent Documents 3 and 4), but still satisfactory. The current situation is that nothing has been obtained.
本発明は、上記の現状に鑑み、化学的に合成された界面活性剤を添加することなく、美肌効果を有する成分のみで、セラミド（スフィンゴ糖脂質）の均一で、安定な水性懸濁液用組成物、並びにその製造方法を提供することを課題とする。
In view of the above situation, the present invention is for a uniform and stable aqueous suspension of ceramide (glycosphingolipid) without adding a chemically synthesized surfactant and only with a component having a skin beautiful effect. An object is to provide a composition and a method for producing the composition.
かかる課題を解決するべく本発明者らは鋭意検討した結果、セラミドとしてのスフィンゴ糖脂質とコラーゲンペプチドを、溶媒中で、特定比率で混合して製した組成物が、均一な水性懸濁状態を良好に呈することを見出し、本発明を完成させるに至った。
As a result of intensive studies conducted by the present inventors to solve such a problem, a composition prepared by mixing glycosphingolipid as ceramide and collagen peptide in a solvent at a specific ratio has a uniform aqueous suspension state. The inventors have found that they exhibit good results and have completed the present invention.
すなわち本発明は、一つの態様として、
（１）スフィンゴ糖脂質及びコラーゲンペプチドの両者を、重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９９：１～２５：７５の割合で含有してなる水中への分散性に優れたスフィンゴ糖脂質組成物；
（２）重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９０：１０～７５：２５の割合で含有してなる上記（１）に記載の水中への分散性に優れたスフィンゴ糖脂質組成物；
（３）組成物中のスフィンゴ糖脂質の含有量が、５～５０重量％である上記（１）又は（２）に記載の水中への分散性に優れたスフィンゴ糖脂質組成物；
（４）乾燥粉末状である上記（１）～（３）のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物；
（５）食品として使用される上記（１）～（４）のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物；
（６）化粧品として使用される上記（１）～（４）のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物；
である。 That is, the present invention, as one aspect,
(1) A glycosphingolipid composition excellent in dispersibility in water, which contains both glycosphingolipid and collagen peptide in a weight ratio of collagen peptide: sphingolipid = 99: 1 to 25:75. object;
(2) The glycosphingolipid composition excellent in dispersibility in water according to the above (1), which contains collagen peptide: sphingolipid in a weight ratio of 90:10 to 75:25.
(3) The glycosphingolipid composition having excellent dispersibility in water according to the above (1) or (2), wherein the content of the glycosphingolipid in the composition is 5 to 50% by weight;
(4) A glycosphingolipid composition which is in the form of a dry powder and has excellent dispersibility in water according to any one of (1) to (3) above;
(5) A glycosphingolipid composition having excellent dispersibility in water according to any one of (1) to (4) above, which is used as a food;
(6) A glycosphingolipid composition having excellent dispersibility in water according to any one of (1) to (4) above, which is used as a cosmetic product.
Is.
（１）スフィンゴ糖脂質及びコラーゲンペプチドの両者を、重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９９：１～２５：７５の割合で含有してなる水中への分散性に優れたスフィンゴ糖脂質組成物；
（２）重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９０：１０～７５：２５の割合で含有してなる上記（１）に記載の水中への分散性に優れたスフィンゴ糖脂質組成物；
（３）組成物中のスフィンゴ糖脂質の含有量が、５～５０重量％である上記（１）又は（２）に記載の水中への分散性に優れたスフィンゴ糖脂質組成物；
（４）乾燥粉末状である上記（１）～（３）のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物；
（５）食品として使用される上記（１）～（４）のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物；
（６）化粧品として使用される上記（１）～（４）のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物；
である。 That is, the present invention, as one aspect,
(1) A glycosphingolipid composition excellent in dispersibility in water, which contains both glycosphingolipid and collagen peptide in a weight ratio of collagen peptide: sphingolipid = 99: 1 to 25:75. object;
(2) The glycosphingolipid composition excellent in dispersibility in water according to the above (1), which contains collagen peptide: sphingolipid in a weight ratio of 90:10 to 75:25.
(3) The glycosphingolipid composition having excellent dispersibility in water according to the above (1) or (2), wherein the content of the glycosphingolipid in the composition is 5 to 50% by weight;
(4) A glycosphingolipid composition which is in the form of a dry powder and has excellent dispersibility in water according to any one of (1) to (3) above;
(5) A glycosphingolipid composition having excellent dispersibility in water according to any one of (1) to (4) above, which is used as a food;
(6) A glycosphingolipid composition having excellent dispersibility in water according to any one of (1) to (4) above, which is used as a cosmetic product.
Is.
また本発明は、別の態様として、
（７）スフィンゴ糖脂質及びコラーゲンペプチドの両者を、重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９９：１～２５：７５の割合で溶媒中混合し、混合物を乾燥粉末状としてなることを特徴とする水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法；
（８）重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９０：１０～７５：２５の割合で混合してなる上記（７）に記載の水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法；
（９）組成物中のスフィンゴ糖脂質含有量が、５～５０重量％である上記（７）又は（８）に記載の水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法；
（１０）溶媒が、水、及び／又はエタノール、あるいはこれらの混合溶媒である、上記（７）～（９）のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法；
である。 The present invention, as another aspect,
(7) Both the glycosphingolipid and the collagen peptide are mixed in a solvent at a weight ratio of collagen peptide: sphingolipid = 99: 1 to 25:75, and the mixture is formed into a dry powder. A method for producing a glycosphingolipid composition having excellent dispersibility in water;
(8) Production of a glycosphingolipid composition excellent in dispersibility in water according to the above (7), which is prepared by mixing collagen peptide: glycosphingolipid = 90: 10 to 75:25 in a weight ratio. Method;
(9) The method for producing a glycosphingolipid composition excellent in dispersibility in water according to the above (7) or (8), wherein the glycosphingolipid content in the composition is 5 to 50% by weight.
(10) Production of a glycosphingolipid composition excellent in dispersibility in water according to any one of (7) to (9) above, wherein the solvent is water and / or ethanol, or a mixed solvent thereof. Method;
Is.
（７）スフィンゴ糖脂質及びコラーゲンペプチドの両者を、重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９９：１～２５：７５の割合で溶媒中混合し、混合物を乾燥粉末状としてなることを特徴とする水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法；
（８）重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９０：１０～７５：２５の割合で混合してなる上記（７）に記載の水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法；
（９）組成物中のスフィンゴ糖脂質含有量が、５～５０重量％である上記（７）又は（８）に記載の水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法；
（１０）溶媒が、水、及び／又はエタノール、あるいはこれらの混合溶媒である、上記（７）～（９）のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法；
である。 The present invention, as another aspect,
(7) Both the glycosphingolipid and the collagen peptide are mixed in a solvent at a weight ratio of collagen peptide: sphingolipid = 99: 1 to 25:75, and the mixture is formed into a dry powder. A method for producing a glycosphingolipid composition having excellent dispersibility in water;
(8) Production of a glycosphingolipid composition excellent in dispersibility in water according to the above (7), which is prepared by mixing collagen peptide: glycosphingolipid = 90: 10 to 75:25 in a weight ratio. Method;
(9) The method for producing a glycosphingolipid composition excellent in dispersibility in water according to the above (7) or (8), wherein the glycosphingolipid content in the composition is 5 to 50% by weight.
(10) Production of a glycosphingolipid composition excellent in dispersibility in water according to any one of (7) to (9) above, wherein the solvent is water and / or ethanol, or a mixed solvent thereof. Method;
Is.
なお、本発明は具体的には上記の態様に関するものであるが、これらに限定されるものではない。
The present invention specifically relates to the above aspects, but is not limited to these.
本発明により、化学的に合成された界面活性剤を使用することなく、美肌効果を有するコラーゲンペプチドのみの添加により、水に対する分散性に優れた、スフィンゴ糖脂質の均一であり安定性に優れた水性懸濁液を調製することができる組成物が提供される。
したがって、本発明が提供するスフィンゴ糖脂質を含有するセラミド組成物は、安全性に優れた組成物として、経口摂取をすることができる機能性食品用組成物、或いは化粧品用の原料組成物として使用することができる点で、極めて有用性の高いものである。 ADVANTAGE OF THE INVENTION According to the present invention, without using a chemically synthesized surfactant, by adding only a collagen peptide having a beautiful skin effect, the dispersibility in water is excellent, and the glycosphingolipid is uniform and excellent in stability. Compositions are provided that allow for the preparation of aqueous suspensions.
Therefore, the ceramide composition containing the glycosphingolipid provided by the present invention is used as a composition with excellent safety, a functional food composition that can be taken orally, or a raw material composition for cosmetics. It is extremely useful in that it can be done.
したがって、本発明が提供するスフィンゴ糖脂質を含有するセラミド組成物は、安全性に優れた組成物として、経口摂取をすることができる機能性食品用組成物、或いは化粧品用の原料組成物として使用することができる点で、極めて有用性の高いものである。 ADVANTAGE OF THE INVENTION According to the present invention, without using a chemically synthesized surfactant, by adding only a collagen peptide having a beautiful skin effect, the dispersibility in water is excellent, and the glycosphingolipid is uniform and excellent in stability. Compositions are provided that allow for the preparation of aqueous suspensions.
Therefore, the ceramide composition containing the glycosphingolipid provided by the present invention is used as a composition with excellent safety, a functional food composition that can be taken orally, or a raw material composition for cosmetics. It is extremely useful in that it can be done.
本発明は、上記したとおり、その基本的態様は、スフィンゴ糖脂質及びコラーゲンペプチドの両者を、重量比率で、コラーゲンペプチド：スフィンゴ糖脂質＝９９：１～２５：７５の割合で含有してなる水中への分散性に優れたスフィンゴ糖脂質組成物である。
As described above, the basic aspect of the present invention is an aqueous solution containing both glycosphingolipid and collagen peptide in a weight ratio of collagen peptide: sphingolipid = 99: 1 to 25:75. A glycosphingolipid composition having excellent dispersibility in water.
本発明で使用するスフィンゴ糖脂質としては、セラミドの一種であり、特にその由来が特に限定されるものではなく、あらゆるスフィンゴ糖脂質を使用することができる。
そのなかでも、スフィンゴ糖脂質としては、植物素材由来のスフィンゴ糖脂質が好ましく、穀類（コメ、コムギ、トウモロコシ、ダイズなど）、果実（モモ、リンゴ、パイナップルなど）、イモ類（コンニャク、ジャガイモ、サツマイモなど）、根菜類（甜菜など）由来のスフィンゴ糖脂質があげられる。 The glycosphingolipid used in the present invention is a kind of ceramide, and its origin is not particularly limited, and any glycosphingolipid can be used.
Among them, glycosphingolipids derived from plant materials are preferable as the glycosphingolipid, and grains (rice, wheat, corn, soybean, etc.), fruits (peach, apple, pineapple, etc.), potatoes (konjac, potato, sweet potato, etc.) are preferred. Etc.), and glycosphingolipids derived from root vegetables (such as sugar beet).
そのなかでも、スフィンゴ糖脂質としては、植物素材由来のスフィンゴ糖脂質が好ましく、穀類（コメ、コムギ、トウモロコシ、ダイズなど）、果実（モモ、リンゴ、パイナップルなど）、イモ類（コンニャク、ジャガイモ、サツマイモなど）、根菜類（甜菜など）由来のスフィンゴ糖脂質があげられる。 The glycosphingolipid used in the present invention is a kind of ceramide, and its origin is not particularly limited, and any glycosphingolipid can be used.
Among them, glycosphingolipids derived from plant materials are preferable as the glycosphingolipid, and grains (rice, wheat, corn, soybean, etc.), fruits (peach, apple, pineapple, etc.), potatoes (konjac, potato, sweet potato, etc.) are preferred. Etc.), and glycosphingolipids derived from root vegetables (such as sugar beet).
より詳細には、このような植物素材由来のスフィンゴ糖脂質、例えば、米糠、米胚芽及び／又はこれら由来のスフィンゴ糖脂質、より好ましくは、スフィンゴ糖脂質がβ－グルコシルセラミド（β－GlcCer）としては、例えば、特開２０１０－２７０１０４号公報に記載する、米糠、米胚芽及び／又はこれら由来の素材を有機溶媒抽出して得たスフィンゴ糖脂質含有粗製画分を、クロマトグラフィー及び／又は再結晶により分画したスフィンゴ糖脂質精製画分からなるスフィンゴ糖脂質（コメセラミド）を挙げることができる。
さらには、特許文献２に従って調製した、桃から得られたスフィンゴ糖脂質（モモセラミド）を挙げることができる。 More specifically, such plant material-derived glycosphingolipid, for example, rice bran, rice germ and / or glycosphingolipid derived therefrom, more preferably, the glycosphingolipid is as β-glucosylceramide (β-GlcCer). Refers to, for example, chromatography and / or recrystallization of a crude fraction containing sphingoglycolipid obtained by organic solvent extraction of rice bran, rice germ and / or materials derived from these, as described in JP 2010-270104A. The glycosphingolipid (come ceramide) consisting of the purified glycosphingolipid fraction obtained by
Further, a glycosphingolipid (peach ceramide) obtained from peach prepared according to Patent Document 2 can be mentioned.
さらには、特許文献２に従って調製した、桃から得られたスフィンゴ糖脂質（モモセラミド）を挙げることができる。 More specifically, such plant material-derived glycosphingolipid, for example, rice bran, rice germ and / or glycosphingolipid derived therefrom, more preferably, the glycosphingolipid is as β-glucosylceramide (β-GlcCer). Refers to, for example, chromatography and / or recrystallization of a crude fraction containing sphingoglycolipid obtained by organic solvent extraction of rice bran, rice germ and / or materials derived from these, as described in JP 2010-270104A. The glycosphingolipid (come ceramide) consisting of the purified glycosphingolipid fraction obtained by
Further, a glycosphingolipid (peach ceramide) obtained from peach prepared according to Patent Document 2 can be mentioned.
一方、本発明の組成物を構成するコラーゲンペプチドは、コラーゲンを酵素などで分解し、比較的低分子化したものである。
かかるコラーゲンペプチドは、肌の保湿性や弾力性を向上するなどの美肌効果や、血液流動性を改善する効果など様々な機能を有するため、近年、多くの飲料、食品、化粧品等に配合されている。 On the other hand, the collagen peptide that constitutes the composition of the present invention is a relatively low molecular weight product obtained by decomposing collagen with an enzyme or the like.
Such collagen peptide has various functions such as skin beautifying effect such as improving the moisturizing property and elasticity of the skin, and an effect of improving blood fluidity, and thus has been blended in many beverages, foods, cosmetics and the like in recent years. There is.
かかるコラーゲンペプチドは、肌の保湿性や弾力性を向上するなどの美肌効果や、血液流動性を改善する効果など様々な機能を有するため、近年、多くの飲料、食品、化粧品等に配合されている。 On the other hand, the collagen peptide that constitutes the composition of the present invention is a relatively low molecular weight product obtained by decomposing collagen with an enzyme or the like.
Such collagen peptide has various functions such as skin beautifying effect such as improving the moisturizing property and elasticity of the skin, and an effect of improving blood fluidity, and thus has been blended in many beverages, foods, cosmetics and the like in recent years. There is.
本発明者らの検討では、スフィンゴ糖脂質に対してコラーゲンペプチドを特定に比率で混合して製した組成物が、スフィンゴ糖脂質を水中に均一に分散させたとき、安定した懸濁溶液となることを確認した。
スフィンゴ糖脂質と混合されるそのようなコラーゲンペプチドとしては、その素材は特に限定されるものではないが、例えば、市販の「ニッピペプタイドＰＲＡ」（株式会社ニッピ）、「水溶性コラーゲンペプチドＳＳ」（協和発酵バイオ株式会社）「コラーゲンペプチドＳＣＰ」（新田ゼラチン株式会社）、「ＨＡＣＰ」（ゼライス株式会社）などを用いることができる。 In the study by the present inventors, a composition prepared by mixing a collagen peptide with a specific ratio of glycosphingolipid is a stable suspension solution when the glycosphingolipid is uniformly dispersed in water. It was confirmed.
The collagen peptide to be mixed with the glycosphingolipid is not particularly limited in its material. For example, commercially available “Nippi peptide PRA” (Nippi Corporation), “water-soluble collagen peptide SS” ( Kyowa Hakko Bio Co., Ltd.) "Collagen peptide SCP" (Nitta Gelatin Co., Ltd.), "HACP" (Zeraisu Co., Ltd.) and the like can be used.
スフィンゴ糖脂質と混合されるそのようなコラーゲンペプチドとしては、その素材は特に限定されるものではないが、例えば、市販の「ニッピペプタイドＰＲＡ」（株式会社ニッピ）、「水溶性コラーゲンペプチドＳＳ」（協和発酵バイオ株式会社）「コラーゲンペプチドＳＣＰ」（新田ゼラチン株式会社）、「ＨＡＣＰ」（ゼライス株式会社）などを用いることができる。 In the study by the present inventors, a composition prepared by mixing a collagen peptide with a specific ratio of glycosphingolipid is a stable suspension solution when the glycosphingolipid is uniformly dispersed in water. It was confirmed.
The collagen peptide to be mixed with the glycosphingolipid is not particularly limited in its material. For example, commercially available “Nippi peptide PRA” (Nippi Corporation), “water-soluble collagen peptide SS” ( Kyowa Hakko Bio Co., Ltd.) "Collagen peptide SCP" (Nitta Gelatin Co., Ltd.), "HACP" (Zeraisu Co., Ltd.) and the like can be used.
本発明が提供するスフィンゴ糖脂質とコラーゲンペプチドとの混合物は、具体的には、スフィンゴ糖脂質及びコラーゲンペプチドの両者を、重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９９：１～２５：７５の割合で溶媒中混合し、混合物を乾燥して粉末状として調製することができる。
より好ましい混合比率としては、重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９０：１０～７５：２５の割合であるのが好ましい。 The mixture of the glycosphingolipid and the collagen peptide provided by the present invention specifically includes both the glycosphingolipid and the collagen peptide in a weight ratio of collagen peptide: sphingolipid = 99: 1 to 25:75. It can be prepared by mixing in a solvent in a ratio and drying the mixture as a powder.
A more preferable mixing ratio is a collagen peptide: glycosphingolipid = 90: 10 to 75:25 ratio by weight.
より好ましい混合比率としては、重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９０：１０～７５：２５の割合であるのが好ましい。 The mixture of the glycosphingolipid and the collagen peptide provided by the present invention specifically includes both the glycosphingolipid and the collagen peptide in a weight ratio of collagen peptide: sphingolipid = 99: 1 to 25:75. It can be prepared by mixing in a solvent in a ratio and drying the mixture as a powder.
A more preferable mixing ratio is a collagen peptide: glycosphingolipid = 90: 10 to 75:25 ratio by weight.
スフィンゴ糖脂質の配合量が、１％未満である場合には、懸濁液の分散性が均一なものとならず、また、７５％を超える場合にも、懸濁液の分散性が均一なものとならないものであった。
なお、組成物中におけるスフィンゴ糖脂質の含有量としては、組成物の全重量を基準として、５～５０重量％であるのが好ましい。
したがって、目的とする組成物の用途に応じてスフィンゴ糖脂質の含有量が選択され、それに応じて本発明の特定の比率に基づくコラーゲンペプチドの量が選択され、両者を溶媒中で均質に混合することにより、本発明の組成物が調製される。 If the content of the glycosphingolipid is less than 1%, the dispersibility of the suspension will not be uniform, and if it exceeds 75%, the dispersibility of the suspension will not be uniform. It was something that was not good.
The content of glycosphingolipid in the composition is preferably 5 to 50% by weight based on the total weight of the composition.
Therefore, the content of the glycosphingolipid is selected according to the intended use of the composition, and the amount of the collagen peptide based on the specific ratio of the present invention is selected accordingly, and both are homogeneously mixed in the solvent. Thereby, the composition of the present invention is prepared.
なお、組成物中におけるスフィンゴ糖脂質の含有量としては、組成物の全重量を基準として、５～５０重量％であるのが好ましい。
したがって、目的とする組成物の用途に応じてスフィンゴ糖脂質の含有量が選択され、それに応じて本発明の特定の比率に基づくコラーゲンペプチドの量が選択され、両者を溶媒中で均質に混合することにより、本発明の組成物が調製される。 If the content of the glycosphingolipid is less than 1%, the dispersibility of the suspension will not be uniform, and if it exceeds 75%, the dispersibility of the suspension will not be uniform. It was something that was not good.
The content of glycosphingolipid in the composition is preferably 5 to 50% by weight based on the total weight of the composition.
Therefore, the content of the glycosphingolipid is selected according to the intended use of the composition, and the amount of the collagen peptide based on the specific ratio of the present invention is selected accordingly, and both are homogeneously mixed in the solvent. Thereby, the composition of the present invention is prepared.
組成物の調製にあたっては、スフィンゴ糖脂質とコラーゲンペプチドの両者を溶媒中で均質に混合して製するのが良く、そのような溶媒としては、水、低級アルコール、多価アルコール、あるいはアセトンなど、またはこれらの２種以上の混合物を挙げることができる。
本発明が提供する組成物は、スフィンゴ糖脂質を経口的に摂取すること、或いは化粧品の原料として使用する点からみて、混合にあたっては毒性の無い溶媒を用いることが必要であり、従って、溶媒としては水、及び／又はエタノールであるのが好ましい。
混合にあたっては、他の溶媒を用いることも可能であるが、安全性が懸念される残留溶媒の問題があり、好ましいものではない。 In the preparation of the composition, it is preferable that both the glycosphingolipid and the collagen peptide are homogeneously mixed and produced in a solvent, and examples of such a solvent include water, a lower alcohol, a polyhydric alcohol, or acetone. Alternatively, a mixture of two or more of these can be used.
The composition provided by the present invention requires ingestion of glycosphingolipids orally, or from the viewpoint of use as a raw material for cosmetics, it is necessary to use a non-toxic solvent for mixing, and therefore, as a solvent. Is preferably water and / or ethanol.
It is possible to use other solvents for the mixing, but it is not preferable because there is a problem of residual solvent which is concerned about safety.
本発明が提供する組成物は、スフィンゴ糖脂質を経口的に摂取すること、或いは化粧品の原料として使用する点からみて、混合にあたっては毒性の無い溶媒を用いることが必要であり、従って、溶媒としては水、及び／又はエタノールであるのが好ましい。
混合にあたっては、他の溶媒を用いることも可能であるが、安全性が懸念される残留溶媒の問題があり、好ましいものではない。 In the preparation of the composition, it is preferable that both the glycosphingolipid and the collagen peptide are homogeneously mixed and produced in a solvent, and examples of such a solvent include water, a lower alcohol, a polyhydric alcohol, or acetone. Alternatively, a mixture of two or more of these can be used.
The composition provided by the present invention requires ingestion of glycosphingolipids orally, or from the viewpoint of use as a raw material for cosmetics, it is necessary to use a non-toxic solvent for mixing, and therefore, as a solvent. Is preferably water and / or ethanol.
It is possible to use other solvents for the mixing, but it is not preferable because there is a problem of residual solvent which is concerned about safety.
混合手段としては、スフィンゴ糖脂質及びコラーゲンペプチドの両者を溶媒中で混合する方法（以下、Ａ法という）、或いは、スフィンゴ糖脂質並びにコラーゲンペプチドのそれぞれの溶液を調製し、スフィンゴ糖脂質の溶液をコラーゲンペプチドの溶液中へ滴下しながら混合する方法（以下、Ｂ法という）により行うことができる。
なお、混合においては、混合液の均質な混合を確保するため、超音波処理をしながら混合することもできる。 As the mixing means, a method of mixing both the glycosphingolipid and the collagen peptide in a solvent (hereinafter referred to as method A), or a solution of each of the glycosphingolipid and the collagen peptide is prepared, and the solution of the glycosphingolipid is prepared. It can be carried out by a method of mixing while dropping into the collagen peptide solution (hereinafter referred to as method B).
In addition, in the mixing, in order to ensure uniform mixing of the mixed liquid, it is possible to perform the mixing while performing ultrasonic treatment.
なお、混合においては、混合液の均質な混合を確保するため、超音波処理をしながら混合することもできる。 As the mixing means, a method of mixing both the glycosphingolipid and the collagen peptide in a solvent (hereinafter referred to as method A), or a solution of each of the glycosphingolipid and the collagen peptide is prepared, and the solution of the glycosphingolipid is prepared. It can be carried out by a method of mixing while dropping into the collagen peptide solution (hereinafter referred to as method B).
In addition, in the mixing, in order to ensure uniform mixing of the mixed liquid, it is possible to perform the mixing while performing ultrasonic treatment.
混合の条件としては、５０℃前後に加温した条件下で０．１～２時間程度混合し、混合終了後減圧下に溶媒を留去し、得られ残留物を均一に粉砕し、これを例えば減圧乾燥することにより目的をする組成物を得ることができる。
As for the mixing condition, mixing is carried out for about 0.1 to 2 hours under the condition of being heated at about 50 ° C., after completion of the mixing, the solvent is distilled off under reduced pressure, and the obtained residue is uniformly pulverized. For example, the desired composition can be obtained by drying under reduced pressure.
これら、Ａ法、或いはＢ法による本発明組成物の調製の詳細を記す。
ただし、以下に説明する方法は、本発明の調製法の一例を具体例として記載するものであって、種々の変法が可能であり、かかる変法も本願発明の目的達成手段として逸脱しない限り、本願発明の技術的範囲に包含されるものである。 The details of the preparation of the composition of the present invention by Method A or Method B will be described below.
However, the method described below describes one example of the preparation method of the present invention as a specific example, and various modified methods are possible, and such modified methods do not depart as the means for achieving the object of the present invention. The invention is included in the technical scope of the present invention.
ただし、以下に説明する方法は、本発明の調製法の一例を具体例として記載するものであって、種々の変法が可能であり、かかる変法も本願発明の目的達成手段として逸脱しない限り、本願発明の技術的範囲に包含されるものである。 The details of the preparation of the composition of the present invention by Method A or Method B will be described below.
However, the method described below describes one example of the preparation method of the present invention as a specific example, and various modified methods are possible, and such modified methods do not depart as the means for achieving the object of the present invention. The invention is included in the technical scope of the present invention.
＜Ａ法＞
ＨＰＬＣ含量分析値をもとに、セラミドであるスフィンゴ糖脂質の含量が１０重量％となるように、米由来セラミド及び魚由来コラーゲンペプチド（ニッピペプタイド）を秤量し、ナス型フラスコヘ移す。その後、９５％エタノールをセラミドとコラーゲンペプチドの総量に対して１０倍量となるように添加し、５０℃に設定した水浴上で２０分間、加温し、混合させる。その後、エバポレーターを用い、溶媒を留去させる。残留物を、乳鉢を用いて、粉砕・均一化させた後、減圧乾燥処理（例えば、８０℃、－０．１ＭＰａ）を行い、溶媒を完全に留去させることにより、本発明の組成物を調製する。 <Method A>
Based on the HPLC content analysis value, the rice-derived ceramide and the fish-derived collagen peptide (nippi peptide) were weighed so that the content of the ceramide glycosphingolipid was 10% by weight, and transferred to an eggplant-shaped flask. Then, 95% ethanol is added so as to be 10 times the total amount of the ceramide and the collagen peptide, and the mixture is heated and mixed in a water bath set at 50 ° C. for 20 minutes. Then, the solvent is distilled off using an evaporator. The residue is pulverized and homogenized using a mortar, and then dried under reduced pressure (for example, 80 ° C., −0.1 MPa) to completely distill off the solvent, whereby the composition of the present invention is obtained. Prepare.
ＨＰＬＣ含量分析値をもとに、セラミドであるスフィンゴ糖脂質の含量が１０重量％となるように、米由来セラミド及び魚由来コラーゲンペプチド（ニッピペプタイド）を秤量し、ナス型フラスコヘ移す。その後、９５％エタノールをセラミドとコラーゲンペプチドの総量に対して１０倍量となるように添加し、５０℃に設定した水浴上で２０分間、加温し、混合させる。その後、エバポレーターを用い、溶媒を留去させる。残留物を、乳鉢を用いて、粉砕・均一化させた後、減圧乾燥処理（例えば、８０℃、－０．１ＭＰａ）を行い、溶媒を完全に留去させることにより、本発明の組成物を調製する。 <Method A>
Based on the HPLC content analysis value, the rice-derived ceramide and the fish-derived collagen peptide (nippi peptide) were weighed so that the content of the ceramide glycosphingolipid was 10% by weight, and transferred to an eggplant-shaped flask. Then, 95% ethanol is added so as to be 10 times the total amount of the ceramide and the collagen peptide, and the mixture is heated and mixed in a water bath set at 50 ° C. for 20 minutes. Then, the solvent is distilled off using an evaporator. The residue is pulverized and homogenized using a mortar, and then dried under reduced pressure (for example, 80 ° C., −0.1 MPa) to completely distill off the solvent, whereby the composition of the present invention is obtained. Prepare.
＜Ｂ法＞
ＨＰＬＣ含量分析値をもとに、セラミドであるスフィンゴ糖脂質の含量が１０重量％となるように、米由来セラミド及び魚由来コラーゲンペプチド（ニッピペプタイド）をそれぞれ別容器に秤量する。セラミドに対しては、エタノール（９９．５％）を秤量値の５倍量となるように添加し、５０℃に設定した水浴上で加温・超音波処理を行う。一方、コラーゲンペプチドに対しては、精製水を秤量値の５倍量となるように添加し、５０℃に設定した水浴上で加温・超音波処理を行う。その後、スターラー攪拌を行いながら、セラミド溶液をコラーゲンペプチド溶液へ滴下する。滴下後、約２０分間混合したのち、エバポレーターを用い、溶媒を留去させる。残留物を、乳鉢を用いて粉砕・均一化させた後、減圧乾燥処理（例えば、８０℃、－０．１ＭＰａ）を行い、溶媒を完全に留去させることにより、本発明の組成物を調製する。 <Method B>
Based on the HPLC content analysis value, the rice-derived ceramide and the fish-derived collagen peptide (nippi peptide) are weighed in separate containers so that the content of the ceramide glycosphingolipid is 10% by weight. To ceramide, ethanol (99.5%) is added so that the amount becomes 5 times the weighed value, and heating / sonication is performed on a water bath set at 50 ° C. On the other hand, to the collagen peptide, purified water is added in anamount 5 times the weighed value, and the mixture is heated and sonicated in a water bath set at 50 ° C. Then, the ceramide solution is added dropwise to the collagen peptide solution while stirring with a stirrer. After the dropping, the mixture is mixed for about 20 minutes, and then the solvent is distilled off using an evaporator. The composition of the present invention is prepared by pulverizing and homogenizing the residue using a mortar, and then performing a vacuum drying treatment (eg, 80 ° C., −0.1 MPa) to completely distill off the solvent. To do.
ＨＰＬＣ含量分析値をもとに、セラミドであるスフィンゴ糖脂質の含量が１０重量％となるように、米由来セラミド及び魚由来コラーゲンペプチド（ニッピペプタイド）をそれぞれ別容器に秤量する。セラミドに対しては、エタノール（９９．５％）を秤量値の５倍量となるように添加し、５０℃に設定した水浴上で加温・超音波処理を行う。一方、コラーゲンペプチドに対しては、精製水を秤量値の５倍量となるように添加し、５０℃に設定した水浴上で加温・超音波処理を行う。その後、スターラー攪拌を行いながら、セラミド溶液をコラーゲンペプチド溶液へ滴下する。滴下後、約２０分間混合したのち、エバポレーターを用い、溶媒を留去させる。残留物を、乳鉢を用いて粉砕・均一化させた後、減圧乾燥処理（例えば、８０℃、－０．１ＭＰａ）を行い、溶媒を完全に留去させることにより、本発明の組成物を調製する。 <Method B>
Based on the HPLC content analysis value, the rice-derived ceramide and the fish-derived collagen peptide (nippi peptide) are weighed in separate containers so that the content of the ceramide glycosphingolipid is 10% by weight. To ceramide, ethanol (99.5%) is added so that the amount becomes 5 times the weighed value, and heating / sonication is performed on a water bath set at 50 ° C. On the other hand, to the collagen peptide, purified water is added in an
かくして調製された本発明の組成物は、水中に均一に分散し、その分散懸濁状態を長時間にわたり安定して維持するものであった。
したがって、本発明が提供する組成物は、保湿効果を目的として経口的に安全に摂取することができ、また、保湿化粧品の原料として、効果的に使用できるものであった。 The thus-prepared composition of the present invention was uniformly dispersed in water and maintained its dispersed suspension state stably for a long time.
Therefore, the composition provided by the present invention can be safely orally taken for the purpose of moisturizing effect, and can be effectively used as a raw material for moisturizing cosmetics.
したがって、本発明が提供する組成物は、保湿効果を目的として経口的に安全に摂取することができ、また、保湿化粧品の原料として、効果的に使用できるものであった。 The thus-prepared composition of the present invention was uniformly dispersed in water and maintained its dispersed suspension state stably for a long time.
Therefore, the composition provided by the present invention can be safely orally taken for the purpose of moisturizing effect, and can be effectively used as a raw material for moisturizing cosmetics.
以下に、本発明を種々の試験例等を記載することにより説明するが。本発明はこれらに限定されるものではない。
The present invention will be described below by describing various test examples and the like. The present invention is not limited to these.
実施例１：Ａ法による本発明の組成物の調製方法
コメ由来スフィンゴ糖脂質（オカヤス株式会社製）２ｇ（ＨＰＬＣ含量８１．８％）と魚由来コラーゲンペプチド（ニッピペプタイド：株式会社ニッピ製）１３ｇに９５％エタノール１５０ｍＬ（１０倍量）を加えて水浴上で撹拌しながら５０℃に加温条件下に２０分間混合した。その後、減圧下でエバポレーターにより溶媒を留去した。残留物を取り出して均一に粉砕し、これを減圧乾燥処理（８０℃、－０．１ＭＰａ）し、目的とする組成物を得た。 Example 1: Method for preparing composition of the present invention by Method A 2 g of rice-derived glycosphingolipid (Okayasu Co., Ltd.) (HPLC content 81.8%) and 13 g of fish-derived collagen peptide (Nippi Peptide: Nippi Co., Ltd.) 150 mL (10 volumes) of 95% ethanol was added to and mixed with heating on a water bath at 50 ° C. for 20 minutes while heating. Then, the solvent was distilled off under reduced pressure with an evaporator. The residue was taken out and uniformly pulverized, and this was dried under reduced pressure (80 ° C., −0.1 MPa) to obtain the desired composition.
コメ由来スフィンゴ糖脂質（オカヤス株式会社製）２ｇ（ＨＰＬＣ含量８１．８％）と魚由来コラーゲンペプチド（ニッピペプタイド：株式会社ニッピ製）１３ｇに９５％エタノール１５０ｍＬ（１０倍量）を加えて水浴上で撹拌しながら５０℃に加温条件下に２０分間混合した。その後、減圧下でエバポレーターにより溶媒を留去した。残留物を取り出して均一に粉砕し、これを減圧乾燥処理（８０℃、－０．１ＭＰａ）し、目的とする組成物を得た。 Example 1: Method for preparing composition of the present invention by Method A 2 g of rice-derived glycosphingolipid (Okayasu Co., Ltd.) (HPLC content 81.8%) and 13 g of fish-derived collagen peptide (Nippi Peptide: Nippi Co., Ltd.) 150 mL (10 volumes) of 95% ethanol was added to and mixed with heating on a water bath at 50 ° C. for 20 minutes while heating. Then, the solvent was distilled off under reduced pressure with an evaporator. The residue was taken out and uniformly pulverized, and this was dried under reduced pressure (80 ° C., −0.1 MPa) to obtain the desired composition.
実施例２：Ｂ法による本発明の組成物の調製方法
コメ由来スフィンゴ糖脂質（オカヤス株式会社製）２ｇ（ＨＰＬＣ含量８１．８％）に９５％エタノール１０ｍＬ（５倍量）を加え水浴上で５０℃に加温しながら超音波処理して完全に溶解した。別途、魚由来コラーゲンペプチド（ニッピペプタイド：株式会社ニッピ製）１３ｇに精製水６５ｍＬ（５倍量）を加えて水浴上で５０℃に加温しながら超音波処理して溶解した。撹拌しながら５０℃に加温下でスフィンゴ糖脂質のエタノール溶液をコラーゲンペプチド水溶液中に少量ずつ滴下して混合し、５０℃、２０分保持した。次いで、減圧下にてエバポレーターで溶媒を留去した。残留物を取り出して均一に粉砕し、これを減圧乾燥処理（８０℃、－０．１ＭＰａ）して、目的物を得た。 Example 2: Method for preparing composition of the present invention by method B 2 g of rice-derived glycosphingolipid (manufactured by Okayasu Co., Ltd.) (HPLC content 81.8%) was mixed with 10 mL of 95% ethanol (5 volumes), and then added on a water bath. While heating to 50 ° C., ultrasonic treatment was performed to completely dissolve it. Separately, purified water (65 mL, 5 times amount) was added to 13 g of fish-derived collagen peptide (Nippi peptide: manufactured by Nippi Co., Ltd.), and ultrasonic treatment was performed while heating to 50 ° C. on a water bath to dissolve. An ethanol solution of glycosphingolipid was added dropwise to the collagen peptide aqueous solution while being stirred and heated at 50 ° C., and the mixture was maintained at 50 ° C. for 20 minutes. Then, the solvent was distilled off under reduced pressure with an evaporator. The residue was taken out and uniformly pulverized, and this was dried under reduced pressure (80 ° C., −0.1 MPa) to obtain the desired product.
コメ由来スフィンゴ糖脂質（オカヤス株式会社製）２ｇ（ＨＰＬＣ含量８１．８％）に９５％エタノール１０ｍＬ（５倍量）を加え水浴上で５０℃に加温しながら超音波処理して完全に溶解した。別途、魚由来コラーゲンペプチド（ニッピペプタイド：株式会社ニッピ製）１３ｇに精製水６５ｍＬ（５倍量）を加えて水浴上で５０℃に加温しながら超音波処理して溶解した。撹拌しながら５０℃に加温下でスフィンゴ糖脂質のエタノール溶液をコラーゲンペプチド水溶液中に少量ずつ滴下して混合し、５０℃、２０分保持した。次いで、減圧下にてエバポレーターで溶媒を留去した。残留物を取り出して均一に粉砕し、これを減圧乾燥処理（８０℃、－０．１ＭＰａ）して、目的物を得た。 Example 2: Method for preparing composition of the present invention by method B 2 g of rice-derived glycosphingolipid (manufactured by Okayasu Co., Ltd.) (HPLC content 81.8%) was mixed with 10 mL of 95% ethanol (5 volumes), and then added on a water bath. While heating to 50 ° C., ultrasonic treatment was performed to completely dissolve it. Separately, purified water (65 mL, 5 times amount) was added to 13 g of fish-derived collagen peptide (Nippi peptide: manufactured by Nippi Co., Ltd.), and ultrasonic treatment was performed while heating to 50 ° C. on a water bath to dissolve. An ethanol solution of glycosphingolipid was added dropwise to the collagen peptide aqueous solution while being stirred and heated at 50 ° C., and the mixture was maintained at 50 ° C. for 20 minutes. Then, the solvent was distilled off under reduced pressure with an evaporator. The residue was taken out and uniformly pulverized, and this was dried under reduced pressure (80 ° C., −0.1 MPa) to obtain the desired product.
実施例３：セラミド含量を変化させた本発明の組成物の調製
スフィンゴ糖脂質の含有比率を１，５，２５，５０，７５％の組成物に関しても、セラミドとコラーゲンペプチドの配合比を変えて、同様の方法（Ａ、Ｂの両法）により、目的とする組成物を調製した。 Example 3: Preparation of a composition of the present invention in which the ceramide content was changed Even for a composition having a glycosphingolipid content ratio of 1, 5, 25, 50, 75%, the compounding ratio of ceramide and collagen peptide was changed. The target composition was prepared by the same method (both methods A and B).
スフィンゴ糖脂質の含有比率を１，５，２５，５０，７５％の組成物に関しても、セラミドとコラーゲンペプチドの配合比を変えて、同様の方法（Ａ、Ｂの両法）により、目的とする組成物を調製した。 Example 3: Preparation of a composition of the present invention in which the ceramide content was changed Even for a composition having a glycosphingolipid content ratio of 1, 5, 25, 50, 75%, the compounding ratio of ceramide and collagen peptide was changed. The target composition was prepared by the same method (both methods A and B).
実施例４：組成物の分散性の評価
上記した各実施例で得られた組成物の水中での分散性を評価した。
＜方法＞
蓋付きの瓶に被検物質が７．５０％（ｗ／ｖ）となるように、被験物質に精製水を加える。試料を約１分間、強く振り混ぜ、混合させる。混合後の試料をすばやく測定セルへ移し、静置後における紫外可視分光光度計により、波長６６０ｎｍにおける透過度を測定する。
測定時間は、静置後０、１、３、５、１０、２０、３０分とし、透過度の変動により分散性を評価した。 Example 4: Evaluation of dispersibility of composition The dispersibility of the composition obtained in each of the above-described examples in water was evaluated.
<Method>
Purified water is added to the test substance so that the test substance is 7.50% (w / v) in a bottle with a lid. The sample is shaken vigorously for about 1 minute to mix. The sample after mixing is quickly transferred to a measuring cell, and the transmittance at a wavelength of 660 nm is measured by an ultraviolet-visible spectrophotometer after standing.
The measurement time was set to 0, 1, 3, 5, 10, 20, 30 minutes after standing, and the dispersibility was evaluated by the change in the transmittance.
上記した各実施例で得られた組成物の水中での分散性を評価した。
＜方法＞
蓋付きの瓶に被検物質が７．５０％（ｗ／ｖ）となるように、被験物質に精製水を加える。試料を約１分間、強く振り混ぜ、混合させる。混合後の試料をすばやく測定セルへ移し、静置後における紫外可視分光光度計により、波長６６０ｎｍにおける透過度を測定する。
測定時間は、静置後０、１、３、５、１０、２０、３０分とし、透過度の変動により分散性を評価した。 Example 4: Evaluation of dispersibility of composition The dispersibility of the composition obtained in each of the above-described examples in water was evaluated.
<Method>
Purified water is added to the test substance so that the test substance is 7.50% (w / v) in a bottle with a lid. The sample is shaken vigorously for about 1 minute to mix. The sample after mixing is quickly transferred to a measuring cell, and the transmittance at a wavelength of 660 nm is measured by an ultraviolet-visible spectrophotometer after standing.
The measurement time was set to 0, 1, 3, 5, 10, 20, 30 minutes after standing, and the dispersibility was evaluated by the change in the transmittance.
＜結果＞
スフィンゴ糖脂質濃度（セラミド濃度）と分散性について、下記表１にその結果を示した。 <Results>
The results of the glycosphingolipid concentration (ceramide concentration) and dispersibility are shown in Table 1 below.
スフィンゴ糖脂質濃度（セラミド濃度）と分散性について、下記表１にその結果を示した。 <Results>
The results of the glycosphingolipid concentration (ceramide concentration) and dispersibility are shown in Table 1 below.
表１：本発明品のスフィンゴ糖脂質濃度と分散性（透過度：６６０ｎｍ）
Table 1: Glycosphingolipid concentration and dispersibility of the product of the present invention (transmittance: 660 nm)
透過度は、スフィンゴ糖脂質が増加するにつれて低下し、２５％で最低値を示し、さらに濃度が増加するにつれて増加していた。
すなわち、透過度の値が低いほど均一な分散性が維持されていることを示している。
静置１０分後の、各濃度における透過度（６６０ｎｍ）を図１に示した。 The permeability decreased with increasing glycosphingolipids, showed a minimum value at 25%, and increased with increasing concentration.
That is, it is indicated that the lower the transmittance value, the more uniform the dispersibility is maintained.
The transmittance (660 nm) at each concentration after 10 minutes of standing was shown in FIG.
すなわち、透過度の値が低いほど均一な分散性が維持されていることを示している。
静置１０分後の、各濃度における透過度（６６０ｎｍ）を図１に示した。 The permeability decreased with increasing glycosphingolipids, showed a minimum value at 25%, and increased with increasing concentration.
That is, it is indicated that the lower the transmittance value, the more uniform the dispersibility is maintained.
The transmittance (660 nm) at each concentration after 10 minutes of standing was shown in FIG.
表１の結果から判断すると、懸濁液の好ましい均一分散性を維持するスフィンゴ糖脂質濃度は、５～７５％であることが判明する（静置１０分後の透過度：５％以下、２０分後の透過度：１０％以下、３０分後の透過度：１０％以下）。
より好ましくは、スフィンゴ糖脂質濃度が１０～２５％であった（静置１０～３０分後の透過度：１％以下）。 Judging from the results shown in Table 1, it is found that the glycosphingolipid concentration that maintains preferable uniform dispersibility of the suspension is 5 to 75% (permeability after 10 minutes of standing: 5% or less, 20 Transmittance after 10 minutes: 10% or less, Transmittance after 30 minutes: 10% or less).
More preferably, the glycosphingolipid concentration was 10 to 25% (permeability after 10 to 30 minutes of standing: 1% or less).
より好ましくは、スフィンゴ糖脂質濃度が１０～２５％であった（静置１０～３０分後の透過度：１％以下）。 Judging from the results shown in Table 1, it is found that the glycosphingolipid concentration that maintains preferable uniform dispersibility of the suspension is 5 to 75% (permeability after 10 minutes of standing: 5% or less, 20 Transmittance after 10 minutes: 10% or less, Transmittance after 30 minutes: 10% or less).
More preferably, the glycosphingolipid concentration was 10 to 25% (permeability after 10 to 30 minutes of standing: 1% or less).
実施例５：透過度の比較検討
上記したように、スフィンゴ糖脂質を含有する組成物の水中での分散性については、溶液として均一性を確保するためには、透過度が低ければ低いほど、溶液の分散性が維持されていることとなる。
そこで、スフィンゴ糖脂質とペプチドコラーゲンの混合物について、本発明により調製した組成物と、単純な両者の混合組成物（単純混合物）との間での透過度の比較を行った。
その結果を、下記表２に示した。 Example 5: Comparative Study of Permeability As described above, regarding the dispersibility of the composition containing the glycosphingolipid in water, the lower the permeability, the better the uniformity as a solution. The dispersibility of the solution is maintained.
Therefore, for the mixture of glycosphingolipid and peptide collagen, the permeability was compared between the composition prepared according to the present invention and a simple mixed composition of both (simple mixture).
The results are shown in Table 2 below.
上記したように、スフィンゴ糖脂質を含有する組成物の水中での分散性については、溶液として均一性を確保するためには、透過度が低ければ低いほど、溶液の分散性が維持されていることとなる。
そこで、スフィンゴ糖脂質とペプチドコラーゲンの混合物について、本発明により調製した組成物と、単純な両者の混合組成物（単純混合物）との間での透過度の比較を行った。
その結果を、下記表２に示した。 Example 5: Comparative Study of Permeability As described above, regarding the dispersibility of the composition containing the glycosphingolipid in water, the lower the permeability, the better the uniformity as a solution. The dispersibility of the solution is maintained.
Therefore, for the mixture of glycosphingolipid and peptide collagen, the permeability was compared between the composition prepared according to the present invention and a simple mixed composition of both (simple mixture).
The results are shown in Table 2 below.
表２：スフィンゴ糖脂質＋コラーゲン混合物と本発明品の水中での分散性の比較
Table 2: Comparison of dispersibility in water of glycosphingolipid + collagen mixture and the product of the present invention
注：Ａ法、Ｂ法：スフィンゴ糖脂質濃度はいずれも１０重量％である。
Note: Method A, Method B: The glycosphingolipid concentration is 10% by weight in both cases.
表中に示したに示した結果からも判明するように、スフィンゴ糖脂質とコラーゲンペプチドは、単純な混合による組成物であっても、溶液の分散性はある程度確保されるものの、本発明の組成物（Ａ法、Ｂ法により調製した組成物）においては、格段に分散性が改善されていることがわかる。この時、表１及び表２から、セラミド１％、コラーゲン９９％であっても、単純混合物よりも分散性が良好であることが判明した。
以上の事実から、本発明の組成物の優位性が理解できるものであった。 As can be seen from the results shown in the table, the glycosphingolipid and the collagen peptide, even if the composition by simple mixing, the dispersibility of the solution is ensured to some extent, but the composition of the present invention. It can be seen that the product (composition prepared by the method A and the method B) has remarkably improved dispersibility. At this time, it was found from Tables 1 and 2 that even 1% ceramide and 99% collagen had better dispersibility than the simple mixture.
From the above facts, the superiority of the composition of the present invention can be understood.
以上の事実から、本発明の組成物の優位性が理解できるものであった。 As can be seen from the results shown in the table, the glycosphingolipid and the collagen peptide, even if the composition by simple mixing, the dispersibility of the solution is ensured to some extent, but the composition of the present invention. It can be seen that the product (composition prepared by the method A and the method B) has remarkably improved dispersibility. At this time, it was found from Tables 1 and 2 that even 1% ceramide and 99% collagen had better dispersibility than the simple mixture.
From the above facts, the superiority of the composition of the present invention can be understood.
本発明が提供する組成物は、その使用目的に悪影響を与えない限り、さらに追加的な成分を含んでもよい。そのような成分は、例えば、ビタミン類、ミネラル類、栄養成分、香料、酸化防止剤、色素類、保存料、調味料、甘味料、酸味料、ｐＨ調整剤、安定剤、等張化剤、乳化剤、緩衝剤、栄養強化剤、防腐剤を挙げることができる。
The composition provided by the present invention may further contain additional components as long as it does not adversely affect the intended purpose. Such ingredients include, for example, vitamins, minerals, nutritional ingredients, flavors, antioxidants, pigments, preservatives, seasonings, sweeteners, acidulants, pH adjusters, stabilizers, tonicity agents, Mention may be made of emulsifiers, buffers, nutrient enhancers and preservatives.
また、本発明が提供する組成物は、スフィンゴ糖脂質及びコラーゲンペプチドに由来する優れた美肌効果を期待できるため、例えば、肌荒れ防止や皮膚の保湿、皮膚の保護、肌のたるみ改善等のために用いられる。
したがって、乾燥肌や肌荒れ、肌のたるみの対象等にとって極めて有用である。 Further, the composition provided by the present invention can be expected to have an excellent skin beautifying effect derived from glycosphingolipids and collagen peptides, and for example, for preventing rough skin and moisturizing the skin, protecting the skin, improving sagging of the skin, etc. Used.
Therefore, it is extremely useful for dry skin, rough skin, and sagging skin.
したがって、乾燥肌や肌荒れ、肌のたるみの対象等にとって極めて有用である。 Further, the composition provided by the present invention can be expected to have an excellent skin beautifying effect derived from glycosphingolipids and collagen peptides, and for example, for preventing rough skin and moisturizing the skin, protecting the skin, improving sagging of the skin, etc. Used.
Therefore, it is extremely useful for dry skin, rough skin, and sagging skin.
かかる目的のために、本発明が提供する組成物は、飲食品として経口的に摂取することもでき、また、化粧品の原料として提供することができる。
本発明の組成物を飲食品として提供する場合、その形態は、機能性表示食品、特定保健用食品（トクホ）、サプリメント、ドリンク剤（溶液剤及び懸濁液剤が含まれる）等の健康食品の形態で提供することも、また、清涼飲料、茶飲料、乳酸菌飲料等の形態で提供することも可能であるが、これらに限定されない。 For this purpose, the composition provided by the present invention can be orally taken as a food or drink, and can be provided as a raw material for cosmetics.
When the composition of the present invention is provided as a food or drink, its form is that of a health food such as a functionally labeled food, a food for specified health use (Tokuho), a supplement, a drink (including a solution and a suspension). It may be provided in the form of a soft drink, a tea drink, a lactic acid bacterium drink, or the like, but is not limited thereto.
本発明の組成物を飲食品として提供する場合、その形態は、機能性表示食品、特定保健用食品（トクホ）、サプリメント、ドリンク剤（溶液剤及び懸濁液剤が含まれる）等の健康食品の形態で提供することも、また、清涼飲料、茶飲料、乳酸菌飲料等の形態で提供することも可能であるが、これらに限定されない。 For this purpose, the composition provided by the present invention can be orally taken as a food or drink, and can be provided as a raw material for cosmetics.
When the composition of the present invention is provided as a food or drink, its form is that of a health food such as a functionally labeled food, a food for specified health use (Tokuho), a supplement, a drink (including a solution and a suspension). It may be provided in the form of a soft drink, a tea drink, a lactic acid bacterium drink, or the like, but is not limited thereto.
以上のとおり本発明により、化学的に合成された界面活性剤を使用することなく、水に対する分散性に優れたスフィンゴ糖脂質の均一な水性懸濁液を調製することができるスフィンゴ糖脂質含有組成物が提供される。
したがって、本発明が提供するスフィンゴ糖脂質含有組成物は、安全性に優れた組成物として、食品用、或いは化粧品用として使用することができる点で、極めて有用性の高いものであり、その産業上に利用性は多大なものである。 As described above, according to the present invention, a glycosphingolipid-containing composition capable of preparing a uniform aqueous suspension of glycosphingolipid having excellent dispersibility in water without using a chemically synthesized surfactant. Things are offered.
Therefore, the glycosphingolipid-containing composition provided by the present invention is extremely useful in that it can be used for foods or cosmetics as a composition having excellent safety. The availability above is enormous.
したがって、本発明が提供するスフィンゴ糖脂質含有組成物は、安全性に優れた組成物として、食品用、或いは化粧品用として使用することができる点で、極めて有用性の高いものであり、その産業上に利用性は多大なものである。 As described above, according to the present invention, a glycosphingolipid-containing composition capable of preparing a uniform aqueous suspension of glycosphingolipid having excellent dispersibility in water without using a chemically synthesized surfactant. Things are offered.
Therefore, the glycosphingolipid-containing composition provided by the present invention is extremely useful in that it can be used for foods or cosmetics as a composition having excellent safety. The availability above is enormous.
Claims (10)
- スフィンゴ糖脂質及びコラーゲンペプチドの両者を、重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９９：１～２５：７５の割合で含有してなる水中への分散性に優れたスフィンゴ糖脂質組成物。 A glycosphingolipid composition excellent in dispersibility in water, which contains both glycosphingolipid and collagen peptide in a weight ratio of collagen peptide: sphingolipid = 99: 1 to 25:75.
- 重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９０：１０～７５：２５の割合で含有してなる請求項１に記載の水中への分散性に優れたスフィンゴ糖脂質組成物。 The glycosphingolipid composition excellent in dispersibility in water according to claim 1, which is contained in a weight ratio of collagen peptide: glycosphingolipid = 90:10 to 75:25.
- 組成物中のスフィンゴ糖脂質の含有量が、５～５０重量％である請求項１又は２に記載の水中への分散性に優れたスフィンゴ糖脂質組成物。 The glycosphingolipid composition excellent in dispersibility in water according to claim 1 or 2, wherein the content of the glycosphingolipid in the composition is 5 to 50% by weight.
- 乾燥粉末状である請求項１～３のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物。 The glycosphingolipid composition having excellent dispersibility in water according to any one of claims 1 to 3, which is a dry powder.
- 食品として使用される請求項１～４のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物。 The glycosphingolipid composition having excellent dispersibility in water according to any one of claims 1 to 4, which is used as a food.
- 化粧品として使用される請求項１～４のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物。 A glycosphingolipid composition having excellent dispersibility in water according to any one of claims 1 to 4, which is used as a cosmetic.
- スフィンゴ糖脂質及びコラーゲンペプチドの両者を、重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９９：１～２５：７５の割合で溶媒中混合し、混合物を乾燥粉末状としてなることを特徴とする水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法。 Both the glycosphingolipid and the collagen peptide are mixed in a solvent in a weight ratio of collagen peptide: sphingolipid = 99: 1 to 25:75 into a solvent, and the mixture is made into a dry powder, which is characterized in that it is in water. A method for producing a glycosphingolipid composition having excellent dispersibility.
- 重量比で、コラーゲンペプチド：スフィンゴ糖脂質＝９０：１０～７５：２５の割合で混合してなる請求項７に記載の水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法。 The method for producing a glycosphingolipid composition having excellent dispersibility in water according to claim 7, wherein the collagen peptide: glycosphingolipid is mixed in a weight ratio of 90:10 to 75:25.
- 組成物中のスフィンゴ糖脂質含有量が、５～５０重量％である請求項７又は８に記載の水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法。 The method for producing a glycosphingolipid composition excellent in dispersibility in water according to claim 7 or 8, wherein the content of the glycosphingolipid in the composition is 5 to 50% by weight.
- 溶媒が、水、及び／又はエタノール、あるいはこれらの混合溶媒である、請求項７～９のいずれかに記載の水中への分散性に優れたスフィンゴ糖脂質組成物の製造方法。 The method for producing a glycosphingolipid composition excellent in dispersibility in water according to any one of claims 7 to 9, wherein the solvent is water and / or ethanol, or a mixed solvent thereof.
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| JP2016067320A (en) * | 2014-09-30 | 2016-05-09 | 小林製薬株式会社 | Edible composition |
| JP2016124798A (en) * | 2014-12-26 | 2016-07-11 | サントリーホールディングス株式会社 | Collagen peptide-containing composition |
| JP2017042084A (en) * | 2015-08-26 | 2017-03-02 | 株式会社明治 | Composition containing collagen peptide and ceramide and method for producing the same |
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| WO2015156246A1 (en) * | 2014-04-10 | 2015-10-15 | サントリーホールディングス株式会社 | Method for masking bitterness of composition containing collagen peptide |
| JP2016067320A (en) * | 2014-09-30 | 2016-05-09 | 小林製薬株式会社 | Edible composition |
| JP2016124798A (en) * | 2014-12-26 | 2016-07-11 | サントリーホールディングス株式会社 | Collagen peptide-containing composition |
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