WO2020068014A3 - Tetrahymena thermophila artificial chromosome 1 (ttac1) and its use for the production of recombinant proteins - Google Patents

Tetrahymena thermophila artificial chromosome 1 (ttac1) and its use for the production of recombinant proteins Download PDF

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Publication number
WO2020068014A3
WO2020068014A3 PCT/TR2019/050498 TR2019050498W WO2020068014A3 WO 2020068014 A3 WO2020068014 A3 WO 2020068014A3 TR 2019050498 W TR2019050498 W TR 2019050498W WO 2020068014 A3 WO2020068014 A3 WO 2020068014A3
Authority
WO
WIPO (PCT)
Prior art keywords
artificial chromosome
seq
recombinant proteins
ttac1
production
Prior art date
Application number
PCT/TR2019/050498
Other languages
French (fr)
Other versions
WO2020068014A2 (en
Inventor
Muhittin ARSLANYOLU
Ayca Fulya ÜSTÜNTANIR DEDE
Original Assignee
Anadolu Universitesi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anadolu Universitesi filed Critical Anadolu Universitesi
Priority to EP19835861.6A priority Critical patent/EP3841210A2/en
Publication of WO2020068014A2 publication Critical patent/WO2020068014A2/en
Publication of WO2020068014A3 publication Critical patent/WO2020068014A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a Tetrahymena thermophila artificial chromosome 1 comprising a telomere sequence having SEQ ID NO: 1. In more preferred embodiments, the artificial chromosome of the present invention further comprises a C3 replicative origin "Tt rDNA C3 ori" (SEQ ID NO: 2), a modified HSP70.2 promoter (SEQ ID NO: 3), a PLA signal sequence (SEQ ID NO: 5), and a sfGFP marker gene (SEQ ID NO: 6) to obtain an artificial chromosome in a circular or linear form. In further aspects, the present invention relates to a novel method for obtaining said artificial chromosome, and utilization of it for producing recombinant proteins.
PCT/TR2019/050498 2018-09-25 2019-06-24 Tetrahymena thermophila artificial chromosome 1 (ttac1) and its use for the production of recombinant proteins WO2020068014A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP19835861.6A EP3841210A2 (en) 2018-09-25 2019-06-24 Tetrahymena thermophila artificial chromosome 1 (ttac1) and its use for the production of recombinant proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR201813880A TR201813880A2 (en) 2018-09-25 2018-09-25 ARTIFICIAL CHROMOSOME OF TETRAHYMENA THERMOPHILA (TtAC1) AND ITS USE FOR RECOMBINANT PROTEIN PRODUCTION
TR2018/13880 2018-09-25

Publications (2)

Publication Number Publication Date
WO2020068014A2 WO2020068014A2 (en) 2020-04-02
WO2020068014A3 true WO2020068014A3 (en) 2020-07-16

Family

ID=69159904

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/TR2019/050498 WO2020068014A2 (en) 2018-09-25 2019-06-24 Tetrahymena thermophila artificial chromosome 1 (ttac1) and its use for the production of recombinant proteins

Country Status (3)

Country Link
EP (1) EP3841210A2 (en)
TR (1) TR201813880A2 (en)
WO (1) WO2020068014A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TR202022424A1 (en) * 2020-12-30 2022-07-21 Eskisehir Teknik Ueniversitesi HETEROLOGICAL EXPRESSION SYSTEM FOR THE PRODUCTION OF RECOMBINANT MAMMINIAN INSULIN AND THE METHOD OF PRODUCTION OF RECOMBINANT MAMMINIAN INSULIN IN TETRAHYMENA THERMOPHILA

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050019924A1 (en) * 1998-07-27 2005-01-27 Hitzeman Ronald A. Automatic eukaryotic artificial chromosome vector
WO2007006812A1 (en) * 2005-07-13 2007-01-18 Cilian Ag Tetrahymena heat inducible promoters and their use
CN101586119A (en) * 2009-05-15 2009-11-25 中国科学院水生生物研究所 Tetrahymena transgenic carrier containing HSP70 promoter and GFP and preparation method and use thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030033617A1 (en) 1996-04-10 2003-02-13 Gyula Hadlaczky Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
DE10105152A1 (en) 2001-01-22 2002-07-25 Hartmann Marcus New nucleic acid encoding ciliate phospholipase A1, useful e.g. for causing secretion of proteins from Tetrahymena

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050019924A1 (en) * 1998-07-27 2005-01-27 Hitzeman Ronald A. Automatic eukaryotic artificial chromosome vector
WO2007006812A1 (en) * 2005-07-13 2007-01-18 Cilian Ag Tetrahymena heat inducible promoters and their use
CN101586119A (en) * 2009-05-15 2009-11-25 中国科学院水生生物研究所 Tetrahymena transgenic carrier containing HSP70 promoter and GFP and preparation method and use thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GUO-LIANG YU ET AL: "Transformation of Tetrahymena thermophila with a mutated circular ribosomal DNA plasmid vector", PROC. NATL. ACAD. SCI. USA, vol. 86, 1 November 1989 (1989-11-01), pages 8487 - 8491, XP055678327 *
HAUSER K ET AL: "Expression of the green fluorescent protein in Paramecium tetraurelia", EUROPEAN JOURNAL OF CELL BIOLOGY, WISSENSCHAFLICHE VERLAGSGESELLSCHAFT, STUTTGART, DE, vol. 79, no. 2, 1 February 2000 (2000-02-01), pages 144 - 149, XP004954617, ISSN: 0171-9335, DOI: 10.1078/S0171-9335(04)70016-3 *
HAYNES W J ET AL: "INDUCTION OF ANTIBIOTIC RESISTANCE IN PARAMECIUM TETRAURELIA BY THEBACTERIAL GENE APH-3'-II", JOURNAL OF EUKARYOTIC MICROBIOLOGY, LAWRENCE, KS, US, vol. 42, no. 1, 1 January 1995 (1995-01-01), pages 83 - 91, XP002044343, ISSN: 1066-5234 *
MOCHIZUKI ET AL: "High efficiency transformation of Tetrahymena using a codon-optimized neomycin resistance gene", GENE, ELSEVIER, AMSTERDAM, NL, vol. 425, no. 1-2, 1 December 2008 (2008-12-01), pages 79 - 83, XP025504483, ISSN: 0378-1119, [retrieved on 20080815], DOI: 10.1016/J.GENE.2008.08.007 *

Also Published As

Publication number Publication date
TR201813880A2 (en) 2020-04-21
WO2020068014A2 (en) 2020-04-02
EP3841210A2 (en) 2021-06-30

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