WO2020056808A1

WO2020056808A1 - Device and system for detecting indocyanine green in blood

Info

Publication number: WO2020056808A1
Application number: PCT/CN2018/109558
Authority: WO
Inventors: 刘旭; 刘吉奎; 黄茜; 姚思宇
Original assignee: 北京大学深圳医院
Priority date: 2018-09-21
Filing date: 2018-10-10
Publication date: 2020-03-26

Abstract

Disclosed are a device and a system for detecting indocyanine green in blood, including a blood shunt tube, a photoelectric detector, and a data acquisition device. The blood shunt tube is made of a transparent material and is connected to an organism to be tested, such that the blood in blood vessels of the organism to be tested is shunted via the blood shunt tube. The photoelectric detector is disposed at a predetermined distance from the blood shunt tube and is used to receive fluorescence emitted from indocyanine green in the blood and convert the fluorescence into an electrical signal. The data acquisition device is connected to the photoelectric detector and is used to acquire the electrical signal.

Description

Device and system for detecting indocyanine green in blood

Technical field

The invention relates to the technical field of biomedical in vitro detection, and in particular, to a device and system for detecting indocyanine green in blood.

Background technique

Indocyanine green (Indocyanine green) is immediately injected into the body through the vein, and immediately binds to plasma proteins. It is quickly distributed in the blood vessels of the whole body with the blood circulation. In the middle, enter the intestine through the biliary tract, and excrete with the feces. Due to rapid excretion, about 97% of normal people are excluded from the blood after 20 minutes of intravenous injection, do not participate in in vivo chemical reactions, no enterohepatic circulation, no lymphatic reflux, and are not excreted from the kidney and other extrahepatic organs. The retention of indocyanine green in the blood after entering the body can well reflect the liver reserve function of the body. Therefore, the determination of the residual amount of indocyanine green in the blood after injection into the body will detect the liver's metabolic capacity and related diseases. Diagnosis is important.

A pulse photometry method is disclosed in the related technology (Wu Jing. (2015). Noninvasive PDD to detect liver effective blood flow in the short-term and prognostic value of patients with chronic acute liver failure. Master's thesis, Guiyang Medical College), in vitro The density of two different light-absorbing substances in the blood was measured and compared, and the indocyanine green concentration injected into the body was analyzed in real time for the diagnosis of liver function reserve and related levels. With this method, individual differences between organisms (for example: differences in the skin and muscles of the organism cause different degrees of fluorescence attenuation when passing through these tissues; and although this difference can be calibrated, it is difficult to achieve accuracy, And time consuming), the related equipment needs to be frequently calibrated and parameter modified.

Summary of the Invention

The present invention aims to solve at least one of the technical problems in the related technology. Therefore, the object of the present invention is to provide an indole cyanide green detection device and system.

To achieve the above object, in one aspect, a device for detecting indolocyanine green in blood according to an embodiment of the present invention includes:

A blood shunt tube, the blood shunt tube is a transparent material, and is used to connect the organism to be tested, so that the blood in the blood vessel of the organism to be tested is shunted through the blood shunt tube;

A photodetector, the photodetector is located at a predetermined distance from the blood shunt tube, and is used to receive fluorescence from indole cyanide green in the blood and convert it into an electrical signal;

A data acquisition device, which is connected to the photodetector and is used to acquire the electrical signal.

In addition, the device for detecting indocyanine green in blood according to the above embodiments of the present invention may also have the following additional technical features:

According to an embodiment of the present invention, one end of the blood shunt tube is adapted to be connected to a first position of a blood vessel of the organism to be detected, and the other end of the blood shunt tube is adapted to be connected to a first position of a blood vessel of the organism to be detected. Two positions, so that the blood flowing out of the first position returns to the blood vessel from the second position after passing through the blood shunt tube.

According to an embodiment of the present invention, a peristaltic pump is further provided. The peristaltic pump is provided on the blood shunt tube and is used for conveying blood in the blood shunt tube.

According to an embodiment of the present invention, a dark room is further included, and the photodetector is disposed in the dark room to reduce interference of external ambient light.

According to an embodiment of the present invention, a first needle is provided on one end of the blood shunt tube, and a second needle is provided on the other end of the blood shunt tube.

According to an embodiment of the present invention, the photodetector is any one of a photomultiplier tube, a silicon photomultiplier, a photodiode, and an avalanche photodiode.

According to an embodiment of the present invention, the predetermined distance is 0.01 mm to 10 cm.

In another aspect, a system for detecting indocyanine green in blood according to an embodiment of the present invention includes:

Device for detecting indocyanine green in blood as described above;

A processing device, the processing device is connected to the data acquisition device, and is configured to determine the content of the indole cyanide green according to the electrical signal.

According to an embodiment of the present invention, a display device is further included, and the display device is connected to the data acquisition device.

According to an embodiment of the present invention, the electrical signal is a pulse signal, and the data acquisition device is specifically configured to periodically collect the number of the pulse signals output by the photodetector in a unit time according to a collection time interval. The processing device is specifically configured to calculate the fluorescence intensity according to the number of the pulse signals, and determine the content of the indole cyan green according to the fluorescence intensity.

According to an embodiment of the present invention, the processing device is further configured to generate indole cyan green data information according to the fluorescence intensity and send it to the display device for display. The indole cyan green data information is about Relationship curve between "fluorescence intensity and measurement time".

According to the device and system for detecting indocyanine green in blood according to the embodiments of the present invention, a blood shunt tube is used to drain blood from an organism to be detected to the outside of the body, and then a photodetector is used to detect the fluorescence emitted by the blood indocyanine green to generate a response. The data acquisition device collects the electrical signal, and the processing device determines the content of indolocyanine green according to the electrical signal. In this way, the detection of the concentration of indolocyanine in blood is realized, and the structure is simple and the detection is convenient; and, Because the blood shunt tube is used to drain blood out of the body for detection, the fluorescence passes through the transparent blood shunt tube and is received by the photodetector without the need to pass through human tissue. Therefore, it can be avoided in related technologies when fluorescence requires traditional body tissue. Absorption and loss, and inaccurate detection results due to individual differences between organisms, as well as the need for correction and parameter revision, etc., the detection results are accurate, high sensitivity, stable and reliable.

Additional aspects and advantages of the present invention will be given in part in the following description, and part of them will become apparent from the following description, or be learned through the practice of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to more clearly explain the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are merely These are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on the structure shown in these drawings without paying creative work.

FIG. 1 is a schematic structural diagram of an indocyanine green detection system in blood according to an embodiment of the present invention.

The realization of the purpose, functional characteristics and advantages of the present invention will be further explained with reference to the embodiments and the drawings.

detailed description

Hereinafter, embodiments of the present invention will be described in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present invention, but should not be construed as limiting the present invention. Based on the embodiments in the present invention, those skilled in the art have not made creative labor prerequisites. All other embodiments obtained below belong to the protection scope of the present invention.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", " Rear "," left "," right "," vertical "," horizontal "," top "," bottom "," inside "," outside "," clockwise "," counterclockwise "," axial "," The azimuth or position relationship indicated by “circumferential” and “radial” is based on the azimuth or position relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must It has a specific orientation, is constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as “first” and “second” may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless specifically defined otherwise.

In the present invention, the terms "installation", "connected", "connected", "fixed" and other terms shall be understood in a broad sense unless otherwise specified and defined, for example, they may be fixed connections or removable connections , Or integrally connected; it can be mechanical or electrical; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

In the present invention, unless specifically stated and defined otherwise, the "first" or "down" of the second feature may include the first and second features in direct contact, and may also include the first and second features. Not directly, but through another characteristic contact between them. Moreover, the first feature is "above", "above", and "above" the second feature, including that the first feature is directly above and obliquely above the second feature, or merely indicates that the first feature is higher in level than the second feature. The first feature is “below”, “below”, and “below” of the second feature, including the fact that the first feature is directly below and obliquely below the second feature, or merely indicates that the first feature is less horizontal than the second feature.

Indocyanine green is a fluorescently labeled drug that is metabolized by the liver. After intravenous injection into the body, the change in the concentration of indocyanine green in the blood directly reflects the liver function of the body. Therefore, the determination of the content of indocyanine green in the blood after injection into the body is of great significance for the detection of the metabolic capacity of the liver and the diagnosis of related diseases. An embodiment of the present invention provides a detection system capable of detecting the content of indocyanine green in the blood.

In the following, an indole cyanide green detection system in blood according to an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

Referring to FIG. 1, a system for detecting indole cyanine green in blood according to an embodiment of the present invention includes an indole cyanide green detection device 100 and a processing device 200 in blood.

Specifically, the indocyanine green detection device 100 in the blood includes a blood shunt tube 10, a photodetector 11, and a data acquisition device 12. The blood shunt tube 10 is made of a transparent material and is used to connect the living body 400 to be tested, so that the blood in the blood vessel of the living body 400 to be shunted through the blood shunt tube 10.

That is, before the test is performed, at least one end of the blood shunt tube 10 is connected to a blood vessel on the organism 400 to be tested, such as a venous blood vessel or an arterial blood vessel. In this way, blood in the blood vessel can flow into the blood shunt outside the body. Tube 10. The blood shunt tube 10 is a transparent material, which can ensure that the fluorescence emitted by indole cyanide green in the blood can be emitted through the blood shunt tube 10.

The photodetector 11 is located at a predetermined distance from the blood shunt tube 10, and is used to receive the fluorescence from indole cyanide green in the blood and convert it into an electrical signal. The photodetector 11 may be any one of a photomultiplier tube, a silicon photomultiplier, a photodiode, and an avalanche photodiode. Preferably, the predetermined distance between the photodetector 11 and the blood shunt tube 10 is 0.01 mm to 10 cm. That is, the photodetector 11 is disposed adjacent to the blood shunt tube 10. The fluorescence emitted by indole cyanide green in the blood can be detected by the photodetector 11 and converted into an electrical signal. The intensity of the electrical signal and the intensity of the fluorescence There is a correlation, for example, the stronger the intensity of the fluorescence, the stronger the intensity of the generated electrical signal.

A data acquisition device 12 is connected to the photodetector 11 to collect the electrical signal, and a processing device 200 is connected to the data acquisition device 12 to determine the content of the indole cyanide green according to the electrical signal.

That is, the data acquisition device 12 can collect the electrical signals output by the photodetector 11. Since the intensity of fluorescence is related to the content of indole cyanide green, for example, the higher the content of indole cyanide green, the stronger the intensity of the fluorescence it emits. Therefore, the content of indocyanine green can be determined by the processing device 200 based on the electrical signal.

According to the indocyanine green detection system provided in the embodiment of the present invention, the blood shunt tube 10 is used to drain blood from the organism 400 to be detected to the outside, and then the photodetector 11 is used to detect the fluorescence generated by the blood indocyanine green. For the corresponding electrical signal, the data acquisition device 12 collects the electrical signal, and the processing device 200 determines the content of indocyanine green according to the electrical signal. In this way, the detection of the concentration of indocyanine green in the blood is realized, and its structure is simple and convenient. And, because blood is drained out of the body for detection using the blood shunt tube 10, the fluorescence passes through the transparent blood shunt tube 10 and is received by the photodetector 11 without the need to pass through human tissue, so fluorescence in the related art can be avoided Absorption and loss when traditional tissues are needed, and inaccurate detection results due to individual differences between organisms, as well as the need for correction and parameter revision, etc. The detection results are accurate, highly sensitive, stable and reliable.

In one embodiment of the present invention, one end of the blood shunt tube 10 is adapted to be connected to a first position of a blood vessel of the organism 400 to be tested, and the other end of the blood shunt tube 10 is adapted to be connected to a blood vessel of the organism 400 to be tested. The second position is such that the blood flowing out of the first position is returned to the blood vessel from the second position after passing through the blood shunt tube 10.

That is, during testing, one end of the blood shunt tube 10 may be connected to a first position (proximal end) of a blood vessel of the organism 400 to be tested, and the other end of the blood shunt tube 10 may be connected to the The second position (distal end) of the blood vessel. In this way, the blood in the blood vessel can flow from the first position into the blood shunt tube 10. After passing through the blood shunt tube 10, it returns to the blood vessel from the second position. In this way, the blood can be ensured. Flow back into the organism 400 to be detected to avoid damage to the organism 400 to be detected.

More specifically, the one end of the blood shunt tube 10 is provided with a first needle (not shown), and the other end of the blood shunt tube 10 is provided with a second needle (not shown). The first and second needles can be arterial or venous needles. During the detection, the first needle is used to penetrate into the first position of the artery or vein, and the second needle is used to penetrate into the second position of the artery or vein. To facilitate the connection between the two ends of the blood shunt tube 10 and the organism 400 to be detected.

According to an embodiment of the present invention, a peristaltic pump 13 is further provided. The peristaltic pump 13 is disposed on the blood shunt tube 10 and is used for conveying blood in the blood shunt tube 10. In this way, the peristaltic pump 13 provides power to transport the blood in the blood shunt tube 10 back into the blood vessel. At the same time, the use of the peristaltic pump 13 can control the blood flow, flow velocity and flow direction in the blood shunt tube 10 to prevent blood backflow. When this happens, the system is more stable.

It can be understood that the peristaltic pump 13 is optional. In some embodiments, the peristaltic pump 13 may not be used, and the blood pressure in the blood vessel may also be used to enable the blood to flow in the blood shunt tube 10.

Optionally, a flow control valve (not shown) may be provided on the blood shunt tube 10, and the flow rate of the blood in the blood shunt tube 10 can be precisely adjusted and controlled by the flow control valve.

In an example of the present invention, a dark room (not shown) is further included, and the photodetector 11 is disposed in the dark room to reduce interference from external ambient light. The dark room can screen the ambient light outside, avoiding the external ambient light from causing interference to the photodetector 11, and ensuring higher detection accuracy of the photodetector 11.

It should be noted that the data acquisition device 12 may be installed in the dark room to form a whole with the photodetector 11, of course, it may also be installed separately in the dark room. In addition, the data collector can be connected to the photodetector 11 through a standard digital interface, such as a USB interface or a serial port.

In addition, the processing device 200 may be a processor (such as an MCU or a CPU), which may be integrated into the data acquisition device 12. The processing device 200 may also be a host computer, and the host computer communicates with the data acquisition device 12.

In an embodiment of the present invention, a display device 300 is further included. The display device 300 is connected to the data acquisition device 12. In this way, the display device 300 can display information such as detection results.

More specifically, the electric signal is a pulse signal, and the data acquisition device 12 is specifically configured to periodically collect the number of the pulse signals output by the photodetector 11 per unit time according to the acquisition time interval, and the processing device 200 is specifically It is used for calculating the fluorescence intensity according to the number of the pulse signals, and determining the content of the indole cyanine green according to the fluorescence intensity. Wherein, optionally, the collection time interval is 0.1us to 200min.

That is, the photodetector 11 outputs pulse signals, and the data acquisition device 12 collects these pulse signals, and then the processing device 200 calculates the fluorescence intensity according to the number of pulse signals. The stronger the fluorescence intensity, the photodetector 11 unit time The greater the number of output pulses, finally, the processing device 200 determines the content of indole cyanine green by the intensity of the fluorescence. In this way, the content of indole cyanide green can be accurately detected.

Further, the processing device 200 is further configured to generate indole cyan green data information according to the fluorescence intensity and send it to the display device 300 for display. The indole cyan green data information is about "fluorescence intensity and measurement Time "relationship curve. In this way, the display device 300 can intuitively know the change process of the content of indocyanine green with time.

In the description of this specification, the description with reference to the terms “one embodiment”, “some embodiments”, “examples”, “specific examples”, or “some examples” and the like means specific features described in conjunction with the embodiments or examples , Structure, material, or characteristic is included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms are not necessarily directed to the same embodiment or example. Moreover, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine different embodiments or examples and features of the different embodiments or examples without conflicting one another.

The above is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structural transformation or direct / indirect use of the description and drawings of the present invention under the inventive concept of the present invention All other related technical fields are included in the patent protection scope of the present invention.

Claims

A device for detecting indocyanine green in blood, comprising:

A blood shunt tube, the blood shunt tube is a transparent material, and is used to connect the organism to be tested, so that the blood in the blood vessel of the organism to be tested is shunted through the blood shunt tube;

A photodetector, the photodetector is located at a predetermined distance from the blood shunt tube, and is used to receive fluorescence from indole cyanide green in the blood and convert it into an electrical signal;

A data acquisition device, which is connected to the photodetector and is used to acquire the electrical signal.
The device for detecting indocyanine green in blood according to claim 1, wherein one end of the blood shunt tube is adapted to be connected to a first position of a blood vessel of the organism to be detected, and the other of the blood shunt tube is One end is adapted to be connected to a second position of a blood vessel of the organism to be detected, so that blood flowing out of the first position flows back into the blood vessel from the second position after passing through the blood shunt tube.
The device for detecting indocyanine green in blood according to claim 1, further comprising a peristaltic pump, wherein the peristaltic pump is provided on the blood shunt tube and is used for conveying blood in the blood shunt tube. .
The device for detecting indole cyanine green in blood according to claim 1, further comprising a dark room, and the photodetector is disposed in the dark room to reduce interference from external ambient light.
The device for detecting indocyanine green in blood according to claim 2, wherein a first needle is provided on one end of the blood shunt tube, and a second needle is provided on the other end of the blood shunt tube. Needle.
The device for detecting indole cyanine green in blood according to claim 1, wherein the photodetector is any one of a photomultiplier tube, a silicon photomultiplier, a photodiode, and an avalanche photodiode.
The device for detecting indocyanine green in blood according to claim 1, wherein the predetermined distance is 0.01 mm to 10 cm.
A detection system for indocyanine green in blood, comprising:

The device for detecting indocyanine green in blood according to any one of claims 1 to 7;

A processing device, the processing device is connected to the data acquisition device, and is configured to determine the content of the indole cyanide green according to the electrical signal.
The device for detecting indocyanine green in blood according to claim 8, further comprising a display device, wherein the display device is connected to the data acquisition device.
The detection device for indole cyanine green in blood according to claim 8 or 9, wherein the electrical signal is a pulse signal, and the data acquisition device is specifically configured to periodically collect unit time according to the acquisition time interval The number of the pulse signals output by the photodetector, the processing device is specifically configured to calculate the fluorescence intensity according to the number of the pulse signals, and determine the indole cyan green according to the fluorescence intensity Content.
The device for detecting indolocyanine green in blood according to claim 10, wherein the processing device is further configured to generate indolocyanine green data information according to the fluorescence intensity and send it to the display device. It is displayed that the indole cyan green data information is a relationship curve about "fluorescence intensity and measurement time".