WO2020014858A1 - 一种基于光纤马赫-曾德尔干涉仪的生物传感器及其制作方法 - Google Patents

一种基于光纤马赫-曾德尔干涉仪的生物传感器及其制作方法 Download PDF

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WO2020014858A1
WO2020014858A1 PCT/CN2018/095933 CN2018095933W WO2020014858A1 WO 2020014858 A1 WO2020014858 A1 WO 2020014858A1 CN 2018095933 W CN2018095933 W CN 2018095933W WO 2020014858 A1 WO2020014858 A1 WO 2020014858A1
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optical fiber
glass tube
cavity
light guide
zehnder interferometer
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PCT/CN2018/095933
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English (en)
French (fr)
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王英
王义平
邵宇
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深圳大学
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Priority to PCT/CN2018/095933 priority Critical patent/WO2020014858A1/zh
Publication of WO2020014858A1 publication Critical patent/WO2020014858A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods

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  • the invention belongs to the field of biosensors, and particularly relates to a biosensor based on an optical fiber Mach-Zehnder interferometer and a manufacturing method thereof.
  • the Mach-Zehnder (MZ) interferometer is an interferometer that can be used to observe the relative phase shift changes produced by the beam emitted from a separate light source into two collimated beams after passing through different paths and the medium. Mach-Zehnder interferometers are widely used in many fields, one of which is the biosensor based on the MZ interferometer.
  • the existing biosensors based on optical fiber MZ interferometers have many shortcomings.
  • Some processing methods require the use of harmful corrosive liquids (such as hydrofluoric acid and other chemical agents) to etch the interior of the optical fiber to form MZ cavities. These corrosive liquids may Damage to the processing personnel, so using this processing method to process the optical fiber is extremely dangerous.
  • a biosensor based on an optical fiber Mach-Zehnder interferometer comprising: a microfluidic chip, a microfluidic channel is provided in the microfluidic chip, and a liquid inlet is provided in the microfluidic chip.
  • the liquid port is in communication with the microfluidic channel; a light guide component provided in the microfluidic channel, an MZ cavity is opened in the lightguide component, and the MZ cavity is in communication with the microfluidic channel;
  • a light source provider on the side of the flow chip, the light source provider is connected to one end of the light guide assembly; and a spectrometer disposed on the side of the micro flow chip, the spectrometer and the light guide assembly are far from the light source One end of the supply is connected.
  • the biological tissue solution is first injected into the microfluidic channel from the liquid inlet, and the biological tissue solution is entered into the MZ cavity in the light guide assembly, and the light signal provided by the light source is provided.
  • the light signal After entering the light guide component, and when the light signal enters the MZ cavity, the light signal simultaneously enters the interference arm formed by the biological tissue solution, and the reference arm formed by the light guide component itself. After the light signal passes through the MZ cavity, it gathers in the light guide component. There is a difference in the refractive index of light between the interference arm and the reference arm, so that the optical signal has a refractive index difference, which causes an optical path difference to form interference.
  • the optical signal is projected from the light exit on the spectrometer to form an interference spectrum.
  • a light guide assembly is used instead of the optical fiber in the prior art. Since the light guide assembly is provided with an MZ cavity, there is no need to corrode the light guide assembly with a hazardous chemical such as hydrofluoric acid. Therefore, in the process of manufacturing a biosensor, The safety of personnel is guaranteed, and the taper treatment of the light guide assembly is not required, which can not only reduce the production Present, but also the diameter of the respective portions of the light guide assembly consistent, thereby reducing light loss and improve the sensitivity of the biosensor, the biosensor to make more reliable.
  • FIG. 1 is a schematic structural diagram of a biosensor based on an optical fiber Mach-Zehnder interferometer according to an embodiment of the present invention, and a cross-sectional process is performed on a microfluidic chip;
  • FIG. 2 is a sectional view of a microfluidic chip and a light guide assembly of a biosensor based on an optical fiber Mach-Zehnder interferometer according to an embodiment of the present invention
  • FIG. 3 is a drift chart of a simulated peak of a biosensor based on a fiber-optic Mach-Zehnder interferometer according to a variation of the refractive index according to an embodiment of the present invention.
  • each reference sign indicates: 1, a microfluidic channel; 101, a syringe; 102, a light source providing part; 103, a liquid inlet; 104, a liquid outlet; 105, a waste liquid collecting device; 106, a spectrometer; 107, microfluidic chip; 11, light inlet; 12, light outlet; 13, light guide assembly; 131, glass tube; 132, communication port; 14, closures; 15, plastic hose; 201, first optical fiber; 202 MZ cavity; 203; filler; 204; second optical fiber.
  • a biosensor based on an optical fiber Mach-Zehnder interferometer comprising: a microfluidic chip, a microfluidic channel is provided in the microfluidic chip, and a liquid inlet is provided in the microfluidic chip.
  • the liquid port is in communication with the microfluidic channel; a light guide component provided in the microfluidic channel, an MZ cavity is opened in the lightguide component, and the MZ cavity is in communication with the microfluidic channel;
  • a light source provider on the side of the flow chip, the light source provider is connected to one end of the light guide assembly; and a spectrometer disposed on the side of the micro flow chip, the spectrometer and the light guide assembly are far from the light source One end of the supply is connected.
  • a biosensor based on an optical fiber Mach-Zehnder interferometer includes: a microfluidic chip 107, a light guide assembly 13, a closure member 14, a light source providing member 102, and a spectrometer 106; a microfluidic chip 107
  • a microfluidic channel 1 is provided inside, and the microfluidic channel 1 communicates with the outside through the light inlet 11 and the light outlet 12, and the microfluidic chip 107 is provided with a liquid inlet 103 and a liquid outlet 104;
  • the light guide assembly 13 is provided in the microcirculation In the channel 1 and the light guide assembly 13 is provided with an MZ cavity 202, and the MZ cavity 202 communicates with the microfluidic channel 1, the biological tissue solution enters the microfluidic channel 1 and the MZ cavity 202 through the liquid inlet 103, and the biological tissue solution After entering the MZ cavity 202, as the interference arm of the biosensor, part of the light guide assembly 13 is used as the reference arm
  • the light source providing member 102 is used to provide a broadband light source; the spectrometer 106 is provided on the side of the microfluidic chip 107 where the light outlet 12 is opened.
  • the spectrometer 106 is provided on the side of the microfluidic chip 107 where the light outlet 12 is opened.
  • the refractive index difference of the broadband light is generated, which causes the optical path difference and forms interference.
  • the broadband light is concentrated in the light guide component 13, It is projected on the spectrometer 106 from the light outlet 12 to form an interference spectrum.
  • the microfluidic channel 1 is a linear channel, and the light inlet 11 and the light outlet 12 are symmetrical. This arrangement can reduce the distance and time required for broadband light to pass through the microfluidic chip 107, thereby reducing light loss. Improve the detection sensitivity of the biosensor.
  • the light guide assembly 13 includes a first optical fiber 201, a light guide, and a second optical fiber 204.
  • the first optical fiber 201 and the second optical fiber 204 are respectively disposed at opposite ends of the light guide, and a cavity is provided inside the light guide, and the cavity As the MZ cavity 202, a filler 203 is filled in the cavity.
  • the first optical fiber 201 and the second optical fiber 204 are both single-mode optical fiber cores. Because the single-mode optical fiber cores have the advantages of low loss and minimum dispersion when transmitting optical signals, the biosensor can be improved. Detection accuracy.
  • the light guide includes: a glass tube 131 and a filler 203; a first optical fiber 201 and a second optical fiber 204 are fused to opposite ends of the glass tube 131, and a cavity in the glass tube 131 serves as an MZ cavity 202, and is opened on the side of the glass tube 131 There are communication ports 132, which extend to the first optical fiber 201 and the second optical fiber 204, respectively, and extend to the opposite ends of the light guide member.
  • the MZ cavity 202 communicates with the outside through the communication port 132; the filling member 203 fills the MZ cavity.
  • the diameter of the filling member 203 is smaller than the diameter of the cavity, so that the biological tissue solution flows into the MZ cavity to form an interference arm.
  • one end of the first optical fiber 201 and the second optical fiber 204 are fused to the glass tube 131 and located on the inner wall of the cavity, and part of the first optical fiber 201 is located in the light inlet of the microfluidic channel 1 On the side of port 11, the second optical fiber 204 is located on the side of light exit port 12 of the microfluidic channel 1.
  • This arrangement is so that after the broadband light passes through the first optical fiber 201, it is convenient for the broadband light to pass through a medium with a different refractive index to generate an optical path difference.
  • Interference is formed, and after the broadband light passes through the glass tube 131, it converges at the second optical fiber 204, and the broadband light that has generated the optical path difference is projected on the spectrometer 106 through the second optical fiber 204, thereby forming an interference spectrum.
  • the filler 203 and the sealing member 14 are both UV-curing adhesive, and the refractive index of the UV-curing adhesive is 1.36.
  • the refractive index of the arm is closer to the refractive index of the aqueous solution, thereby reducing the refractive index of the biosensor provided by the present invention, so that the sensitivity of the biosensor provided by the present invention is 40,000nm / RIU, while the sensitivity of a common biosensor on the market is 10000nm / RIU, This improves the sensitivity of the biosensor.
  • the UV-curing glue When using UV-curing glue as the closure member 14, the UV-curing glue has the advantages of fast curing, low energy consumption, and no solvent pollution, which can reduce the curing time of the glue, reduce the waste of energy, and reduce the negative impact on the biological tissue solution. Wait.
  • the length of the glass tube 131 is 100 ⁇ m
  • the inner and outer diameters of the glass tube 131 are 125 ⁇ m and 75 ⁇ m, respectively
  • the communication opening 132 opened by the glass tube 131 is formed by excluding the thickness of 40 ⁇ m on the side of the glass tube 131.
  • a plastic hose 15 is provided at the liquid inlet 103 of the microfluidic chip 107.
  • the use of the plastic hose 15 can more easily introduce the biological tissue solution into the microfluidic channel 1.
  • a syringe 101 is used to The plastic tissue 15 is injected with a biological tissue solution at a speed to introduce the biological tissue solution into the microfluidic channel 1.
  • a waste liquid collection device 105 is provided at the liquid outlet 104 of the microfluidic chip 107. After obtaining an interference spectrum of a biological tissue solution, the biological tissue solution in the microfluidic chip 107 is introduced into the waste liquid collection device 105 and used. The microfluidic channel 1 and the light guide assembly 13 are cleaned by ethanol, and then the ethanol is introduced into the waste liquid collection device 105.
  • the waste liquid collection device 105 can temporarily store the waste liquid generated from multiple experiments, thereby preventing the experimenters from having to perform each experiment. The waste liquid needs to be put into the waste liquid drop-in point, which reduces the labor intensity of the experimenter, and reduces the time for the experimenter to put waste liquid, thereby improving the efficiency of multiple consecutive experiments.
  • the present invention also provides a method for manufacturing a biosensor based on an optical fiber Mach-Zehnder interferometer.
  • the method includes: fusing the first optical fiber 201 to one end of a glass tube 131, and performing precision cutting processing on the glass tube 131.
  • the glass tube 131 is kept at a length of 100 ⁇ m, and then the second optical fiber 204 is welded on the other end of the glass tube 131; one side of the glass tube 131 is removed, and the thickness is 40 ⁇ m, so that a communication port 132 is opened on the glass tube 131,
  • the cavity inside the glass tube 131 communicates with the outside world; the glass tube 131 is filled with ultraviolet curing adhesive, and the ultraviolet curing adhesive is cured, and the diameter of the cured ultraviolet curing adhesive is smaller than the diameter of the cavity in the glass tube 131.
  • the present invention provides a biosensor based on an optical fiber Mach-Zehnder interferometer and a manufacturing method thereof.
  • the working principle or process is as follows: When manufacturing a biosensor, the light guide component 13 is first placed in a microfluidic channel of a microfluidic chip 107 1, then use ultraviolet curing adhesive to seal the light inlet 11 and light outlet 12 at both ends of the microfluidic channel 1, and then connect the end of the first optical fiber 201 away from the glass tube 131 with the light source provider 102, and connect the second optical fiber
  • the end of 204 far from the glass tube 131 is connected to the spectrometer 106, so that the broadband light provided by the light source provider 102 can be projected on the spectrometer 106 through the first optical fiber 201, the glass tube 131 and the second optical fiber 204, and then the plastic is softened.
  • One end of the tube 15 is fixed at the liquid inlet 103 of the microfluidic chip 107, so that the plastic hose 15 communicates with the microfluidic channel 1 through the liquid inlet 103, and finally a waste liquid is installed at the liquid outlet 104 of the microfluidic chip 107
  • the collection device 105 completes the production of the biosensor. Therefore, during the production process, the safety of the production personnel is guaranteed.
  • the light source provider 102 When using a biosensor, first extract the biological tissue solution with a syringe 101, then connect the syringe 101 to a plastic hose 15, and inject the biological fluid into the microfluidic channel 1 through the plastic hose 15 and the liquid inlet 103 at a predetermined rate. Tissue solution. After the biological tissue solution enters the microfluidic channel 1 and the MZ cavity 202, the light source provider 102 is activated, so that the light source provider 102 provides broadband light, and the broadband light is transmitted in the first fiber core.
  • the broadband light When the broadband light reaches the MZ cavity 202 At the same time, the interference arm formed by the biological tissue solution and the reference arm formed by the filling member 203 are simultaneously entered, and the round is at the interference fiber and the second optical fiber 204 at the end of the reference arm. Due to the refractive index difference between the interference arm and the reference arm, Therefore, the broadband light produces a difference in optical path length and forms interference. After the broadband light is projected on the spectrometer 106, an interference spectrum is formed. After the spectrum is stabilized, the data is recorded, and then the ethanol is injected into the microfluidic chip 107 to clean the microfluidic channel 1 and MZ.
  • Cavity 202 and the generated waste liquid is introduced into the waste liquid collection device 105, and then another biological tissue solution with a different concentration is injected into the microfluidic channel 1 and the MZ cavity 202, and tested sequentially
  • the spectra of biological tissue solutions of different concentrations are filled with a UV curable adhesive with a refractive index of 1.36 as a reference arm in the MZ cavity 202, so that the refractive index of the reference arm is closer to the refractive index of the aqueous solution, so that the present invention is based on
  • the fiber-optic Mach-Zehnder interferometer has a high detection sensitivity.
  • the present invention provides The fiber-optic Mach-Zehnder interferometer-based biosensor has higher refractive index sensitivity and lower refractive index detection limit, so it also shows excellent sensing characteristics in detecting the concentration of biological tissue solutions.
  • the cavity in the glass tube is used as the MZ cavity, thereby eliminating the need to use hydrofluoric acid, etc.
  • Dangerous chemical agents corrode the optical fiber, so the safety of the producers is guaranteed during the process of making the biosensor; and the glass tube is filled by using a filler with a refractive index of 1.36, so that the biosensor provided by the present invention
  • the sensitivity is 40000nm / RIU, which is 4 times the sensitivity of most biosensors on the market, thereby improving the sensitivity of biosensors in the prior art.

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Abstract

本发明提供了一种基于光纤马赫-曾德尔干涉仪的生物传感器，用于检测生物组织溶液的浓度，避免了制作人员在制作过程中可能承受的风险，提高了生物传感器可靠度和灵敏度；该生物传感器包括：微流芯片（107），微流芯片（101）内开设有微流通道（1），微流芯片（107）开设有进液口（103），进液口（103）与微流通道（1）连通；设置在微流通道（1）内的光导组件（13），光导组件（13）开设有MZ腔（202），MZ腔（202）与微流通道（1）连通；设置在微流芯片（107）一侧的光源提供件（102）；以及，设置在微流芯片（107）一侧的光谱仪（106）；光导组件（13）的设置，避免了在制作生物传感器的过程中危险腐蚀液体的使用，从而使制作人员安全得到了保障。一种所述生物传感器的制作方法。

Description

一种基于光纤马赫-曾德尔干涉仪的生物传感器及其制作方法 技术领域
本发明属于生物传感器领域，尤其涉及一种基于光纤马赫-曾德尔干涉仪的生物传感器及其制作方法。
 背景技术
马赫-曾德尔（Mach-Zehnder，MZ）干涉仪是一种干涉仪，可以用来观测从单独光源发射的光束分裂成两道准直光束后经过不同路径与介质所产生的相对相移变化。马赫-曾德尔干涉仪在众多领域具有广泛应用，其中一项应用就是基于MZ干涉仪的生物传感器。然而，现有的基于光纤MZ干涉仪的生物传感器存在诸多缺陷。在制作生物传感器的过程中，需要对光纤进行加工处理，有的加工方法需要利用有害腐蚀液体（如：氢氟酸等化学药剂）对光纤内部进行腐蚀以形成MZ腔，而这些腐蚀液体可能会对加工人员造成伤害，从而使用此加工方法对光纤进行加工处理存在极大的危险。
 技术问题
现有的基于光纤MZ干涉仪的生物传感器在制作过程中，制作人员的安全得不到保障。
 技术解决方案
一种基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于，包括：微流芯片，所述微流芯片内开设有微流通道，所述微流芯片开设有进液口，所述进液口与所述微流通道相连通；设置在所述微流通道内的光导组件，所述光导组件内开设有MZ腔，所述MZ腔与所述微流通道相连通；设置在所述微流芯片一侧的光源提供件，所述光源提供件与所述光导组件的一端相连接；以及，设置在所述微流芯片一侧的光谱仪，所述光谱仪与所述光导组件远离所述光源提供件的一端相连接。
 有益效果
当使用基于光纤马赫-曾德尔干涉仪的生物传感器时，先将生物组织溶液从进液口注入微流通道内，并使生物组织溶液进入光导组件内的MZ腔，在光源提供件提供的光信号进入光导组件后，且当光信号进入MZ腔时，光信号同时进入生物组织溶液形成的干涉臂，以及光导组件自身形成的参考臂，在光信号经过MZ腔后，在光导组件内聚集，由于干涉臂及参考臂之间的光折射率存在差异，从而使光信号产生折射率差，从而产生光程差，形成干涉，最后光信号从出光口投射在光谱仪上，从而形成干涉光谱，在本发明中，使用光导组件代替了现有技术中的光纤，由于光导组件开设有MZ腔，故无需用氢氟酸等危险化学药剂对光导组件进行腐蚀，所以在制作生物传感器的过程中，使制作人员的安全性得到了保障，并且无需对光导组件进行拉锥处理，不仅能够减少制作成本，而且能够使光导组件的各部位直径保持一致，从而减少光损耗，提高了生物传感器的灵敏度，使生物传感器更加可靠。
 附图说明
图1是本发明实施例基于光纤马赫-曾德尔干涉仪的生物传感器的结构示意图，并对微流芯片做剖视处理；
图2是本发明实施例基于光纤马赫-曾德尔干涉仪的生物传感器的微流芯片及光导组件的剖视图；
图3是本发明实施例基于光纤马赫-曾德尔干涉仪的生物传感器的模拟仿真波峰随折射率变化的漂移图。
在附图中，各附图标记表示：1、微流通道；101、注射器；102、光源提供件；103、进液口；104、出液口；105、废液收集装置；106、光谱仪；107、微流芯片；11、进光口；12、出光口；13、光导组件；131、玻璃管；132、连通口；14、封闭件；15、塑料软管；201、第一光纤；202、MZ腔；203、填充件；204、第二光纤。
 本发明的最佳实施方式
一种基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于，包括：微流芯片，所述微流芯片内开设有微流通道，所述微流芯片开设有进液口，所述进液口与所述微流通道相连通；设置在所述微流通道内的光导组件，所述光导组件内开设有MZ腔，所述MZ腔与所述微流通道相连通；设置在所述微流芯片一侧的光源提供件，所述光源提供件与所述光导组件的一端相连接；以及，设置在所述微流芯片一侧的光谱仪，所述光谱仪与所述光导组件远离所述光源提供件的一端相连接。
 本发明的实施方式
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合附图及实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。
一种基于光纤马赫-曾德尔干涉仪的生物传感器，如图1及图2所示，包括：微流芯片107、光导组件13、封闭件14、光源提供件102及光谱仪106；微流芯片107内开设有微流通道1，微流通道1通过进光口11及出光口12与外界相连通，且微流芯片107开设有进液口103及出液口104；光导组件13设置在微流通道1内，且光导组件13内开设有MZ腔202，并且MZ腔202与微流通道1相连通，生物组织溶液通过进液口103进入微流通道1及MZ腔202内，并且生物组织溶液进入MZ腔202内后，作为生物传感器的干涉臂，部分光导组件13作为生物传感器的参考臂；封闭件14设置在微流芯片107上，用于在将光导组件13置于微流通道1内后，将出光口12及进光口11封闭，从而防止生物组织溶液从出光口12及进光口11流出微流芯片107，从而降低生物组织溶液对光导组件13造成负面影响的几率，同时能够防止生物组织溶液的浪费，以及降低生物组织溶液对微流芯片107腐蚀的几率；光源提供件102设置在微流芯片107开设进光口11的一侧，用于提供光源，为提高光源的光谱宽度，以提高生物传感器的测量灵敏度，在本实施例中，光源提供件102用于提供宽带光源；光谱仪106设置在微流芯片107开设出光口12的一侧，当光源提供件102提供的宽带光同时经过干涉臂及参考臂时，由于干涉臂及参考臂之间的光折射率存在差异，从而使宽带光产生折射率差，从而产生光程差，形成干涉，最后宽带光在光导组件13内聚集，并从出光口12投射在光谱仪106上，从而形成干涉光谱。
在本实施例中，微流通道1为直线通道，并且进光口11及出光口12相对称，这样设置，能够减少宽带光穿过微流芯片107需要的路程和时间，从而减少光损耗，提高生物传感器的探测灵敏度。
其中，光导组件13包括：第一光纤201、光导件及第二光纤204，第一光纤201及第二光纤204分别设置在光导件相对的两端，光导件内部开设有空腔，该空腔作为MZ腔202，填充件203填充于空腔内。
在本实施例中，第一光纤201及第二光纤204均为单模光纤纤芯，由于单模光纤纤芯在传输光信号时，具有低损耗及最小色散的优点，从而能够提高生物传感器的探测精度。
光导件包括：玻璃管131及填充件203；第一光纤201及第二光纤204熔接于玻璃管131相对的两端，玻璃管131内的腔室作为MZ腔202，在玻璃管131的侧面开设有连通口132，连通口132分别向第一光纤201及第二光纤204延伸，并且延伸至光导件相对的两端，MZ腔202通过连通口132与外界相连通；填充件203填充于MZ腔202内，作为生物传感器的参考臂，并且填充件203的直径小于空腔的直径，以便于生物组织溶液流入MZ腔而形成干涉臂。
在本实施例中，第一光纤201及第二光纤204熔接于玻璃管131的一端位于空腔的内壁上，并且部分与填充件203相接触，第一光纤201位于微流通道1的进光口11一侧，第二光纤204位于微流通道1的出光口12一侧，这样设置，在宽带光经过第一光纤201后，便于宽带光经过不同折射率的介质而产生光程差，从而形成干涉，在宽带光穿过玻璃管131后，在第二光纤204处汇合，已产生光程差的宽带光经过第二光纤204投射在光谱仪106上，从而形成干涉光谱。
如图2及图3所示，在本实施例中，填充件203及封闭件14均为紫外固化胶，紫外固化胶的折射率为1.36，当使用紫外固化胶作为填充件203时，使参考臂的折射率更加接近水溶液的折射率，从而降低了本发明提供的生物传感器的折射率，使得本发明提供的生物传感器灵敏度为40000nm/RIU，而市场上常见的生物传感器灵敏度为10000nm/RIU，从而提高了生物传感器的灵敏度。当使用紫外固化胶作为封闭件14时，由于紫外固化胶具有固化快、耗能少、无溶剂污染等优点，从而能够减少胶水固化的时间，减少能源的浪费，降低对生物组织溶液造成负面影响等。
在本实施例中，玻璃管131的长度为100μm，玻璃管131的内径及外径分别为125μm及75μm，玻璃管131开设的连通口132为抛除玻璃管131一侧40μm的厚度形成。
在微流芯片107的进液口103处设置有塑料软管15，塑料软管15的使用，能够更加便于将生物组织溶液导入微流通道1内，在本实施例中，使用注射器101以预定速度向塑料软管15注射生物组织溶液，以将生物组织溶液导入微流通道1。
在微流芯片107的出液口104处设置有废液收集装置105，在得到一份生物组织溶液的干涉光谱后，将微流芯片107内的生物组织溶液导入废液收集装置105内，并用乙醇清洗微流通道1及光导组件13，随后将乙醇导入废液收集装置105内，废液收集装置105的设置，能够暂时存储多次实验所产生的废液，从而防止实验人员每次实验都需要将废液投放至废液投放点，降低了实验人员的劳动强度，并减少了实验人员投放废液的时间，从而提高连续多次实验的效率。
本发明还提供了一种基于光纤马赫-曾德尔干涉仪的生物传感器的制作方法，上述方法包括：将第一光纤201与玻璃管131的一端相熔接，并对玻璃管131做精密切割处理，使玻璃管131保留100μm的长度，随后在玻璃管131的另一端熔接第二光纤204；将玻璃管131的一侧抛除，抛除厚度为40μm，从而在玻璃管131上开设连通口132，以使玻璃管131内部的空腔与外界相连通；在玻璃管131内填充紫外固化胶，并使紫外固化胶固化，且使固化后的紫外固化胶的直径小于玻璃管131内的空腔直径；移除玻璃管131内多于的紫外固化胶，以使玻璃管131内部的紫外固化胶达到最佳干涉光强对比度；在微流芯片107上制作微流通道1，并开设进液口103及出液口104；将玻璃管131、第一光纤201及第二光纤204置于微流通道1内，并用紫外固化胶分别将微流通道1两端的进光口11及出光口12封闭；将第一光纤201远离玻璃管131的一端与光源提供件102相连接，将第二光纤204远离玻璃管131的一端与光谱仪106相连接。
本发明提供的一种基于光纤马赫-曾德尔干涉仪的生物传感器及其制作方法，其工作原理或过程如下：在制作生物传感器时，先将光导组件13置于微流芯片107的微流通道1内，随后用紫外固化胶将微流通道1两端的进光口11及出光口12密封，然后将第一光纤201远离玻璃管131道的一端与光源提供件102相连接，将第二光纤204远离玻璃管131道的一端与光谱仪106相连接，从而使光源提供件102提供的宽带光能够通过第一光纤201、玻璃管131及第二光纤204而投射在光谱仪106上，随后将塑料软管15的一端固定在微流芯片107的进液口103处，使塑料软管15通过进液口103与微流通道1相连通，最后在微流芯片107的出液口104处安装废液收集装置105，从而完成生物传感器的制作，因此在制作的过程中，保障了制作人员的安全性。
当使用生物传感器时，先用注射器101抽取生物组织溶液，随后将注射器101与塑料软管15相连接，并在预定速率下通过塑料软管15及进液口103向微流通道1内注射生物组织溶液，在生物组织溶液进入微流通道1及MZ腔202后，启动光源提供件102，使光源提供件102提供宽带光，宽带光在第一纤芯内传输，当宽带光到达MZ腔202时，同时进入生物组织溶液形成的干涉臂，以及填充件203形成的参考臂，并在干涉臂及参考臂末端的第二光纤204处回合，由于干涉臂及参考臂之间存在折射率差，故宽带光产生光程差，形成干涉，并在宽带光投射在光谱仪106上后，形成干涉光谱，待光谱稳定后记录数据，随后换乙醇注入微流芯片107内，清洗微流通道1及MZ腔202，并将产生的废液导入废液收集装置105内，然后换另一种不同浓度的生物组织溶液注入微流通道1及MZ腔202内，并依次测试不同浓度的生物组织溶液的光谱图，通过在MZ腔202内填充折射率为1.36的紫外固化胶作为参考臂，使参考臂的折射率更加接近于水溶液的折射率，从而使本发明提供的基于光纤马赫-曾德尔干涉仪的生物传感器具有较高的探测灵敏度。
不同浓度的生物组织溶液的密度存在差异，从而在折射率方面存在差异，而不同浓度的生物组织溶液注入MZ腔202后，改变了干涉臂的折射率，从而导致干涉臂及参考臂之间的折射率差发生变化，从而使干涉光谱发生波长漂移，通过检测干涉光谱的波长漂移，能够间接检测出所测量的生物组织溶液的浓度变化，在使用本发明所提供的生物传感器后，使本发明提供的基于光纤马赫-曾德尔干涉仪的生物传感器具有较高的折射率灵敏度及较低的折射率探测极限，因此在探测生物组织溶液浓度方面也表现出优良传感特性。
以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等，均应包含在本发明的保护范围之内。
 工业实用性
通过采用玻璃管两端熔接光纤，然后从侧面抛磨开腔的方法代替现有技术中MZ干涉仪的生物传感器内的空腔，使玻璃管内的空腔作为MZ腔，从而无需采用氢氟酸等危险化学药剂对光纤进行腐蚀，所以在制作生物传感器的过程中，使制作人员的安全性得到了保障；并且通过使用折射率为1.36的填充件对玻璃管进行填充，使得本发明提供的生物传感器灵敏度为40000nm/RIU，为市场上的大多数生物传感器灵敏度的4倍，从而提高了现有技术中生物传感器的灵敏度。

Claims (10)

  1.   一种基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于，包括：微流芯片(107)，所述微流芯片(107)内开设有微流通道(1)，所述微流芯片(107)开设有进液口(103)，所述进液口(103)与所述微流通道(1)相连通；
    设置在所述微流通道(1)内的光导组件(13)，所述光导组件(13)内开设有MZ腔(202)，所述MZ腔(202)与所述微流通道(1)相连通；
    设置在所述微流芯片（107）一侧的光源提供件(102)，所述光源提供件(102)与所述光导组件(13)的一端相连接；以及，
    设置在所述微流芯片（107）一侧的光谱仪(106)，所述光谱仪(106)与所述光导组件(13)远离所述光源提供件(102)的一端相连接。
  2.   根据权利要求1所述的基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于：所述光导组件(13)包括：第一光纤(201)；
    设置在所述第一光纤(201)一端的光导件，所述光导件内部开设有空腔，所述空腔为MZ腔(202)；以及，
    设置在所述光导件远离所述第一光纤(201)一端的第二光纤(204)，所述第一光纤(201)及所述第二光纤(204)沿所述光导件对称设置。
  3.   据权利要求2所述的基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于：所述光导件包括：
    玻璃管(131)，所述玻璃管(131)的相对两端分别与所述第一光纤(201)及所述第二光纤(204)相熔接，所述玻璃管(131)侧面开设有连通口(132)，所述连通口(132)两端分别向所述第一光纤(201)及所述第二光纤(204)延伸，且延伸至所述玻璃管(131)相对的两端，所述MZ腔(202)通过所述连通口(132)与外界相连通；以及，
    填充于MZ腔(202)内的填充件(203)，所述填充件(203)的直径小于所述MZ腔(202)的直径，所述第一光纤(201)及所述第二光纤(204)熔接于所述玻璃管(131)的一端均位于所述空腔的内壁上，且部分与所述填充件(203)相接触。
  4.   根据权利要求3所述的基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于：所述填充件(203)为紫外固化胶，所述紫外固化胶的折射率为1.36。
  5.   根据权利要求3所述的基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于：所述玻璃管(131)长度为100μm，所述玻璃管(131)外径及内径分别为125μm及75μm。
  6.   根据权利要求2所述的基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于：所述第一光纤(201)及所述第二光纤(204)为单模光纤纤芯。
  7.   根据权利要求1所述的基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于：所述光源提供件(102)用于提供宽带光源。
  8.   根据权利要求1所述的基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于：所述微流芯片（107）开设的进液口（103）处设置有塑料软管(15)。
  9.   根据权利要求1所述的基于光纤马赫-曾德尔干涉仪的生物传感器，其特征在于：所述微流芯片（107）开设有出液口（104），所述出液口（104）与所述微流通道（1）相连通，所述微流芯片（107）一侧设置有废液收集装置(105)，所述废液收集装置（105）与所述出液口（104）相连通。
  10.   一种基于光纤马赫-曾德尔干涉仪的生物传感器的制作方法，其特征在于，所述方法包括：
    将第一光纤(201)与玻璃管(131)的一端相熔接，并对玻璃管(131)做切割处理，随后在玻璃管(131)的另一端熔接第二光纤(204)；
    将玻璃管(131)的一侧抛除，从而在玻璃管(131)上开设连通口(132)，以使玻璃管(131)内部的空腔与外界相连通；
    在玻璃管(131)内填充紫外固化胶，并使紫外固化胶固化，且使固化后的紫外固化胶的直径小于玻璃管(131)内的空腔直径；
    移除玻璃管(131)内多余的紫外固化胶，以使玻璃管(131)内部的紫外固化胶达到最佳干涉光强对比度；
    在微流芯片(107)上制作微流通道(1)，并开设进液口(103)及出液口(104)；
    将玻璃管(131)、第一光纤（201）及第二光纤（204）置于微流通道(1)内，并用紫外固化胶分别将微流通道(1)两端的进光口(11)及出光口(12)封闭；
    将第一光纤（201）远离玻璃管的一端与光源提供件（102）相连接，将第二光纤（204）远离玻璃管（131）的一端与光谱仪(106)相连接。
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