WO2019182148A1 - Biological tissue collecting instrument, biological tissue collecting device, and biological tissue collecting method - Google Patents

Abstract

Provided are a biological tissue collecting instrument, a biological tissue collecting device, and a biological tissue collecting method with which it is possible to collect a biological tissue with reduced destruction of tissue structure. The biological tissue collecting instrument of the present invention comprises a first cutting unit (1) and a second cutting unit (2), and is characterized in that: the first cutting unit (1) cuts a biological tissue to form a side surface of the cutting tissue; the second cutting unit (2) during rotation cuts the biological tissue to form a bottom surface of the cutting tissue; the second cutting unit (2) is disposed in the first cutting unit (1); and the bottom surface is in a surface direction intersecting the side surface.

Description

Biological tissue collection device, biological tissue collection device, and biological tissue collection method

The present invention relates to a biological tissue collection instrument, a biological tissue collection device, and a biological tissue collection method.

When performing biological tissue examination (hereinafter also referred to as “biopsy”), tissue sampling is performed using a biopsy needle or the like. When a biopsy needle is used, after puncturing the living tissue, suction is performed under reduced pressure, and the living tissue around the tip of the biopsy needle is sucked into the needle to collect the living tissue (Non-Patent Document 1). However, the tissue structure of the biological tissue obtained by suction collection is destroyed at the time of collection. For this reason, there exists a problem that a biological tissue cannot be extract | collected in the state which maintained the original tissue structure.

Therefore, an object of the present invention is to provide a biological tissue collection instrument, a biological tissue collection device, and a biological tissue collection method that can collect a biological tissue with reduced destruction of the tissue structure.

In order to achieve the above object, the biological tissue collection device of the present invention (hereinafter also referred to as “collection device”) includes a first cutting unit and a second cutting unit,
The first cutting unit cuts the living tissue to form a side surface of the cut tissue;
The second cutting unit, when rotating, cuts the living tissue to form a bottom surface of the cut tissue,
The second cutting unit is disposed in the first cutting unit;
The bottom surface is a surface direction intersecting with the side surface.

The biological tissue collection device of the present invention (hereinafter also referred to as “collection device”) includes the biological tissue collection device of the present invention, a moving unit, and a rotation unit,
The moving unit is capable of moving the biological tissue collection device forward and backward from the biological tissue;
The rotating unit can rotate the second cutting unit.

The biological tissue collection method of the present invention (hereinafter also referred to as “collection method”) includes introducing the biological tissue collection device of the present invention into a biological tissue, cutting the biological tissue with the first cutting unit, and cutting the tissue. A first cutting step for forming a side surface of
A second cutting step of rotating the biological tissue collection device, and cutting the biological tissue cut by the first cutting unit to form a bottom surface of the cut tissue by the second cutting unit;
A recovery step of extracting the biological tissue collection device out of the biological tissue and recovering the cut tissue.

According to the present invention, it is possible to collect a living tissue (cut tissue) with reduced destruction of the tissue structure.

FIG. 1 is a view showing an example of a sampling device of the present invention, (A) is a perspective view, (B) is a cross-sectional view in the direction AA ′, and (C) is a cross-sectional view of B- FIG. 4 is a cross-sectional view in the B ′ direction, (D) is a cross-sectional view in the CC ′ direction, and (E) is an exploded view. FIG. 2 is a diagram showing another example of the sampling device of the present invention, (A) is a perspective view, (B) is a cross-sectional view in the direction of AA ′, (C) is FIG. 4 is a cross-sectional view in the BB ′ direction, (D) is a cross-sectional view in the CC ′ direction, and (E) is an exploded view. FIG. 3 is a view showing another example of the sampling device of the present invention, (A) is a perspective view, (B) is a cross-sectional view in the direction AA ′, (C) is FIG. 4 is a cross-sectional view in the BB ′ direction, (D) is a cross-sectional view in the CC ′ direction, and (E) is an exploded view. FIG. 4 is a view showing another example of the sampling device of the present invention, (A) is a perspective view, (B) is a cross-sectional view in the direction of AA ′, (C) is FIG. 4 is a cross-sectional view in the BB ′ direction, (D) is a cross-sectional view in the CC ′ direction, and (E) is an exploded view. FIG. 5 is a view showing another example of the sampling device of the present invention, (A) is a perspective view, (B) is a sectional view in the DD ′ direction, and (C) is It is sectional drawing of an EE 'direction. FIG. 6 is a view showing another example of the sampling device of the present invention, (A) is a perspective view, (B) is a cross-sectional view in the DD ′ direction, and (C) is It is sectional drawing of an EE 'direction. FIG. 7 is a view showing another example of the sampling device of the present invention, (A) is a perspective view, (B) is a cross-sectional view in the DD ′ direction, (C), It is sectional drawing of an EE 'direction. FIG. 8 is a view showing another example of the sampling device of the present invention, (A) is a perspective view, (B) is a cross-sectional view in the DD ′ direction, and (C) is It is sectional drawing of an EE 'direction. FIG. 9 is a perspective view showing an example of the collection device of the present invention, (A) is a perspective view, and (B) is an enlarged view of the collection device 100 in (A).

<Biological tissue collection device>
As described above, the biological tissue collection device of the present invention includes a first cutting unit and a second cutting unit, and the first cutting unit cuts the biological tissue to form a side surface of the cut tissue, The second cutting unit, when rotating, cuts the living tissue to form a bottom surface of the cut tissue, the second cutting unit is disposed in the first cutting unit, and the bottom surface intersects the side surface. It is a surface direction. In the collection instrument of the present invention, the first cutting unit cuts the living tissue to form a side surface of the cutting tissue, and the second cutting unit is cut by the first cutting unit during rotation. To form a bottom surface of the cut tissue, the second cutting unit is disposed in the first cutting unit, and the bottom surface is a surface direction intersecting the side surface, The conditions are not particularly limited. For the biological tissue collection device of the present invention, for example, the description of the collection device and collection method of the present invention described later can be used.

In the sampling device of the present invention, for example, when the sampling device is first introduced into the living tissue, the first cutting unit cuts the living tissue with the introduction, and then rotates the second unit. With this rotation, the second cutting unit further cuts the living tissue. At this time, the first cutting unit forms, for example, a side surface of the finally obtained cut tissue in order to cut the living tissue along the direction of introduction of the sampling device to the living tissue, and the second The cutting unit forms the bottom surface of the cut tissue. In the cut tissue, the bottom surface is a surface in a direction intersecting the side surface. In the cut tissue, the surface facing the bottom surface is, for example, the surface of the biological tissue (for example, epithelial tissue such as skin) when the sampling device of the present invention is introduced into the biological tissue. The living tissue to be introduced may be, for example, a living tissue separated from a living body or a living body. For this reason, the cut tissue can be separated from the living tissue by forming the side surface and the bottom surface, for example. In addition, the collection device of the present invention can be implemented without aspiration of the living tissue, for example, in forming the cut tissue. Therefore, according to the collection device of the present invention, it is possible to collect a biological tissue with reduced destruction of the tissue structure. The “reduction of tissue structure destruction” means that, for example, a layer structure or a hierarchical structure in the living tissue is maintained. As a specific example, for example, in the case of a biological tissue in which epithelial tissue, connective tissue, and muscle tissue are laminated in this order from the outside of the living tissue toward the inside of the living tissue, in the cut tissue, the epithelial tissue, When the connective tissue and the muscle tissue are laminated in this order, it can be said that the layer structure or the hierarchical structure is maintained. On the other hand, in the cut tissue, when the order of the epithelial tissue, the connective tissue, and the muscle tissue is changed, or when two or more tissues are not independent layers but mixed, for example, the layer structure Or it can be said that the hierarchical structure is not maintained, that is, the organizational structure is destroyed. In the case where the cutting by the second cutting unit is performed after the cutting by the first cutting unit, that is, the case where the bottom of the cut tissue is formed by cutting the biological tissue cut by the first cutting unit. Although described above, the present invention is not limited to this. In the sampling device of the present invention, for example, the second cutting unit may cut the living tissue simultaneously with or prior to cutting by the first cutting unit.

The sampling device of the present invention can form the bottom surface of the cut tissue by the second cutting unit. In the case of a sampling device that does not have the second cutting unit, such as the biopsy needle described above, the sampling device cannot form the bottom surface of the cut tissue, for example. For this reason, when the said collection instrument can penetrate the said biological tissue, since the said collection instrument can make the other surface of the said biological tissue facing the said surface into the said bottom face, the said cut | disconnected tissue can be extract | collected. However, in the case of a collection tool that does not have the second cutting unit, if the collection tool cannot penetrate the living tissue, the collection tool cannot form the bottom surface, for example, and can form only the side surface. In this case, the cut tissue is connected to the living tissue at a portion corresponding to the bottom surface, for example. For this reason, the cut tissue cannot be collected, for example. On the other hand, the sampling device of the present invention can form the bottom surface by the second cutting unit even when the living tissue cannot be penetrated by the sampling device of the present invention. For this reason, the sampling device of the present invention can form the cut tissue from the biological tissue by forming the side surface and the bottom surface even for a biological tissue having a thickness that cannot be penetrated by the sampling device of the present invention. Separable. For this reason, according to the collection instrument of the present invention, for example, the cut tissue can be collected from a living tissue having an arbitrary thickness.

The sampling device of the present invention can separate a part of an object from the object, for example. For this reason, the collection device of the present invention can also be referred to as a “biological tissue separation device”, for example. The object is not particularly limited, and examples thereof include solid cancers such as breast cancer and skin cancer. When the collection device of the present invention is used for the separation of the cancer, the collection device of the present invention can also be referred to as a “surgical device”, for example. The collection device of the present invention can also be used as a collection device for living tissue in a medical device such as an endoscope. In this case, the collection device of the present invention can also be referred to as a “medical device”, for example.

In the present invention, as described above, the side surface and the bottom surface may be in a plane direction in which the bottom surface intersects with the side surface, that is, the side surface and the bottom surface intersect each other. ing. For example, the side surface and the bottom surface can further reduce the destruction of the tissue structure of the cut tissue. Therefore, the bottom surface has an intersection angle of 90 ± 30 degrees, 90 ± 20 degrees, 90 ± 10 degrees, 90 ± It is 1 degree and is preferably substantially orthogonal or orthogonal.

In the present invention, various “units” can be read as, for example, “means” or “mechanism”.

In the present invention, the biological tissue is not particularly limited, and examples thereof include biological tissue used for biopsy. As a specific example, the biological tissue is, for example, cartilage, skin tissue, subcutaneous tissue, blood vessel tissue, liver tissue, periosteum, synovium, mucous membrane, periodontal membrane membrane tissue, thyroid gland, adrenal gland, hormone producing tissue such as prostate, Examples include nervous tissue and tendons. The living tissue may be an organ, for example. Examples of the organ include a breast. The origin of the living body and the living tissue may be, for example, a human or a non-human animal such as a dog, a cat, a pig, a cow, a monkey, a bird, or a sheep.

The first cutting unit and the second cutting unit of the sampling device of the present invention will be described with reference to FIGS.

FIG. 1 is a schematic view showing an example of a sampling device of the present invention. 1A is a perspective view, FIG. 1B is a cross-sectional view in the direction AA ′, FIG. 1C is a cross-sectional view in the direction BB ′, and FIG. , CC ′ direction sectional view, (E) is an exploded view. As shown in FIG. 1, the collection tool 100 includes a first cutting unit 1 and a second cutting unit 2. The first cutting unit 1 includes a blade 11 that is a first cutting unit that cuts the living tissue, and a cylinder 12 that is a holding unit that holds the cut tissue. The blade 11 is formed in the opening of the tube 12. The second cutting unit 2 includes a blade 21 that is a second cutting unit that cuts the living tissue, and a support unit 22 that supports the cutting unit. The blade 21 is formed at the opening side end of the cylinder 12 in the support portion 22. As shown by an arrow in FIG. 1 (E), the second cutting unit 2 is detachably disposed in the first cutting unit 1. As shown in FIGS. 1B and 1D, in the second cutting unit 2, the side surfaces of the blade 21 and the support portion 22 are inscribed with the blade 11 and the cylinder 12, respectively. As shown in FIG. 1B, the blade 21 is arranged so as to cross the cross section in the cross section with respect to the axial direction of the tube 12. Further, the blade 21 is disposed so as to pass through the center (center of gravity) of the cross section of the cylinder 12.

When the biological tissue is cut by the first cutting unit 1 of the collection tool 100, the biological tissue or the living body is arranged at the tip of the arrow X direction, and the biological tissue is moved by the blade 11 by moving the collection tool 100 in the arrow X direction. The side surface of the cut tissue is formed by cutting (first cutting step). At this time, the living tissue is cut by the blade 21. Next, the second cutting unit 2 is rotated in the circumferential direction of the cylinder 12 in a state where the collection tool 100 is introduced into the living tissue. Thereby, a biological tissue is cut | disconnected by the blade 21, and the bottom face of the said cut | disconnected tissue is formed (2nd cutting process). The second cutting unit 2 may be rotated by rotating the collection tool 100, for example. The rotation angle in the circumferential direction may be an angle at which the bottom surface of the cut tissue is formed, for example. As a specific example, the rotation angle of the second cutting unit 2 is, for example, 180 ° or more. The side surface formed by the blade 11 and the bottom surface cut by the blade 21 intersect each other. That is, the bottom surface is connected to the side surface, for example. For this reason, the cut tissue is separated from surrounding biological tissue. Therefore, the cut tissue is collected (collected), for example, by returning the collection tool 100 in the direction opposite to the arrow X direction (collection step).

In the collection tool 100, the first cutting unit 1 includes a blade 11 that is a first cutting unit that cuts the living tissue and a cylinder 12 that is a holding unit that holds the cut tissue. Any other configuration may be adopted as long as the side surface of the cut tissue can be formed by cutting the living tissue. As a specific example, the 1st cutting unit 1 may have only the blade 11 which is the said 1st cutting part, for example.

In the sampling device 100, the blade 11 is formed over the entire opening of the tube 12, but may be formed in part. By forming the blade 11 over the entire opening of the tube 12, for example, the resistance of the sampling device 100 when cutting the living tissue can be reduced, and the tissue of the living tissue (cutting tissue) generated by an excessive force during cutting. Structural destruction can be reduced. When the holding part has a shape other than the cylinder 12, the first cutting part is formed at one end of the holding part, more specifically, on the introduction end side of the holding part into the living tissue. In the collection tool 100, the blade 11 is a double-edged blade, but may be a single-edged blade. The sampling instrument 100 can reduce the resistance at the time of cutting | disconnecting the said biological tissue by the sampling instrument 100 by using the blade 11 as a double-edged blade, for example. For this reason, the collection instrument 100 can further reduce the destruction of the tissue structure of the living tissue caused by an excessive force during cutting. The angle of the blade of the blade 11 is not particularly limited, and is, for example, 10 to 120 °, 10 to 60 °, 60 to 90 °, 90 to 120 °. The angle of the blade means, for example, an angle at which the other blade surface intersects one blade surface. The thickness of the blade 11 can be appropriately set according to, for example, the strength (for example, hardness, elasticity) of the material of the blade 11. For example, the living body generated by an excessive force at the time of cutting can be reduced. The thinner one is preferable because the destruction of the tissue structure of the tissue (cut tissue) can be reduced. The thickness of the blade 11 is, for example, 10 to 500 μm, and preferably 30 to 500 μm.

In the collection tool 100, the cylinder 12 as the holding portion is a cylinder, but may be any structure as long as it can hold the cut tissue. Examples of the holding portion include a circular or square cylinder or box having a circular cross section. When the holding part is a cylinder, the holding part may have a bottomed cylindrical shape, for example. In this case, the first cutting part is formed in the opening of the bottomed cylinder. In addition, the bottom portion preferably has a hole, for example, because the cut tissue in the holding portion can be easily led out of the holding portion. For example, the holding portion preferably has no opening on the side surface. The said side surface means the surface of the circumferential direction of the said holding part, for example. Since the holding portion does not have an opening on the side surface, the sampling device 100 can reduce the destruction of the tissue structure of the living tissue caused by friction with the cut tissue, for example, when rotating. The diameter (diameter) of the holding part is not particularly limited, and can be appropriately set according to the diameter of the cut tissue to be collected. The diameter (diameter) of the holding portion is, for example, 0.2 to 50 mm, 0.2 to 10 mm, or 10 to 50 mm. The length of the holding part is not particularly limited, and can be set according to the depth from the surface of the biological tissue to be collected. The length of the holding portion is, for example, 20 to 300 mm, 20 to 100 mm, or 100 to 300 mm. The length means, for example, a distance in the axial direction of the holding portion. When the collection device 100 is used as a biological tissue collection device, the diameter (diameter) of the holding portion is preferably 0.1 to 0.9 mm, 1 to 3 mm, and 4 to 6 mm. When using the sampling instrument 100 as a surgical instrument, the diameter (diameter) of the holding part is preferably 3 to 30 mm, 3 to 5 mm, 5 to 10 mm, and 10 to 30 mm.

In the collection tool 100, the second cutting unit 2 includes a blade 21 that is a second cutting unit that cuts the living tissue, and a support unit 22 that supports the blade 21, and the second cutting unit 2 includes: For example, it is sufficient if the living tissue cut by the first cutting unit 1 can be cut to form the bottom surface of the cut tissue during rotation, and other configurations may be adopted. As a specific example, the 2nd cutting unit 2 may have only the blade 21 which is the said 2nd cutting part, for example. In the collection tool 100, the second cutting unit 2 is inscribed in the first cutting unit 1, but it may not be inscribed. By arranging the first cutting unit 1 so as to be inscribed in the second cutting unit 2, the sampling device 100 can efficiently form the bottom surface, for example, and can reduce the destruction of the tissue structure of the living tissue.

In the collection tool 100, the second cutting unit 2 is detachably disposed in the first cutting unit 1, but may be fixed in the first cutting unit 1 or formed integrally with the first cutting unit 1. May be. By fixing the second cutting unit 2 in the first cutting unit 1 or integrally forming it with the first cutting unit 1, the sampling device 100 can apply force when rotating the sampling device 100 to the blade 11 and the blade, for example. It becomes easy to transmit to 21. For this reason, by adopting such a configuration, the sampling device 100 can easily collect the cut tissue even when the biological tissue to be collected exists at a deep position from the surface, for example. The second cutting unit 2 can be fixed in the first cutting unit 1 using, for example, a fixing member. Examples of the fixing member include a spring.

In the sampling device 100, the blade 21 is inscribed in the first cutting unit 1, but it does not have to be inscribed. By arranging the blade 21 as the second cutting part so as to be inscribed in the first cutting unit 1, the sampling device 100 can efficiently form the bottom surface, for example, and destroy the tissue structure of the living tissue. Can be further reduced. Moreover, although the blade 21 is arranged linearly so as to cross the cross section in the cross section with respect to the axial direction of the cylinder 12, it may be arranged in a curved shape. The cross section is preferably, for example, a cross section that is substantially orthogonal or orthogonal to the first cutting unit 1, more specifically, the axial direction of the holding portion. The substantially orthogonal direction means, for example, that the crossing angle between the axial direction and the cross section is 90 ± 30 degrees, 90 ± 20 degrees, 90 ± 10 degrees, 90 ± 1 degrees. By setting the cross section in the substantially orthogonal direction, for example, when the bottom surface is formed by the second cutting unit 2, the sampling device 100 can further reduce the destruction of the tissue structure of the biological tissue on the bottom surface side. The blade 21 is arranged so as to pass through the center of the cross section in the cross section, but the blade 21 may not pass through the center of the cross section. By arranging the blade 21 so as to pass through the center of the cross section, the sampling device 100 can efficiently form the bottom surface, for example, and can further reduce the destruction of the tissue structure of the living tissue.

In the sampling instrument 100, the blade 21 is disposed at the same position as the blade 11 in the axial direction of the cylinder 12 (left and right direction in FIG. 1E), but may be disposed at a different position. As a specific example, the blade 21 may be arranged in the axial direction of the tube 12 in the inner direction of the tube 12 than the blade 11 (right direction in FIG. 1E), or in the outer direction of the tube from the blade 11 (see FIG. 1 (E) left direction).

In the sampling device 100, the blade 21 is a double blade, but it may be a single blade. The sampling instrument 100 can reduce the resistance when the biological tissue is cut by the sampling instrument 100, for example, by using the blade 21 as a double-edged blade. For this reason, the collection instrument 100 can further reduce the destruction of the tissue structure of the living tissue caused by an excessive force during cutting. The angle of the blade of the blade 21 is not particularly limited, and is 15 to 180 °, 15 to 30 °, 30 to 90 °, and 90 to 180 °. The angle of the blade means, for example, an angle at which the other blade surface intersects one blade surface. The thickness of the blade 21 can be appropriately set according to, for example, the strength (for example, hardness, elasticity) of the material of the blade 21, but, for example, the resistance at the time of cutting can be reduced, and the living body generated by an excessive force at the time of cutting. The thinner one is preferable because the destruction of the tissue structure of the tissue (cut tissue) can be reduced. The thickness of the blade 21 is, for example, 10 to 2000 μm, and preferably 30 to 1000 μm.

In the sampling device 100, the support portion 22 is a rectangular plate, but may be any shape as long as it can support the blade 21 as the second cutting portion. The size of the support portion 22 is not particularly limited, and can be set as appropriate based on the size and shape of the holding portion.

In the collection tool 100, the entire second cutting unit 2 is disposed in the first cutting unit 1, but a part thereof may be disposed in the first cutting unit 1. When a part of the second cutting unit 2 is arranged in the first cutting unit 1, for example, a part of the second cutting unit 2 is directed from the inside of the first cutting unit 1 to the outside of the first cutting unit 1. It protrudes.

In the collection tool 100, the forming material of each component is not particularly limited, and is a resin such as a metal such as stainless steel or titanium, plastic, polyvinyl chloride, Teflon (registered trademark), polyurethane, polyacetal, polyether ether ketone (PEEK), or the like. And carbon such as carbon fiber. As the forming material, for example, stainless steel is preferable because force can be efficiently transmitted to the distal end portion of the second cutting unit 2 at the time of the rotation operation and the manufacturing cost can be reduced. For example, the collection tool 100 may be entirely formed of the same forming material.

In the sampling tool 100, the first cutting unit 1 is cylindrical, the second cutting unit 2 is plate-shaped, and the second cutting unit 2 can be detachably disposed on the first cutting unit 1, The shapes and configurations of the first cutting unit 1 and the second cutting unit 2 are not limited to this. In the present invention, the first cutting unit 1 has, for example, one or more recesses such as grooves on the inner surface thereof, and the second cutting unit 2 is fitted into the recesses so that the inside of the first cutting unit 1 May be arranged. In this case, the contact surface in the fitting portion between the first cutting unit 1 and the second cutting unit may be joined by welding or the like, for example. Other examples of the first cutting unit 1 and the second cutting unit 2 will be described with reference to FIGS. The first cutting unit 1 and the second cutting unit 2 in FIGS. 2 to 8 can be combined. 2 to 8 are schematic views showing other examples of the sampling device of the present invention. 2 to 4, (A) is a perspective view, (B) is a sectional view in the direction AA ′, (C) is a sectional view in the direction BB ′, D) is a cross-sectional view in the CC ′ direction, and (E) is an exploded view. 2 to 4, the left figure of (E) is a plan view of the first cutting unit 1, the upper right figure of (E) is a plan view of the second cutting unit 2, and (E) The lower right figure is a right side view of the second cutting unit 2. 5 to 8, (A) is a perspective view, (B) is a sectional view in the D-D 'direction, and (C) is a sectional view in the E-E' direction.

As shown in FIG. 2, the sampling instrument 110 has a first cutting unit 1 and a second cutting unit 2. In the sampling instrument 110, the cylinder 12 that is the holding portion of the first cutting unit 1 is tapered on the end of the opening where the blade 11 is formed. Specifically, the cylinder 12 has a tapered shape that narrows toward the blade 11, for example. By making the 1st cutting | disconnection unit 1 into such a structure, the collection instrument 110 can reduce the resistance at the time of cut | disconnecting the said biological tissue with the collection instrument 110, for example. For this reason, the collection instrument 110 can further reduce the destruction of the tissue structure of the living tissue caused by an excessive force during cutting. The taper angle (intersection angle between two-dot chain lines L1 and L2 in FIG. 2E) is, for example, 10 to 30 °. Except for these points, the sampling instrument 110 has the same configuration as the sampling instrument 100, and the description thereof can be used.

As shown in FIG. 3, the sampling instrument 120 has a first cutting unit 1 and a second cutting unit 2. In the sampling instrument 120, the cylinder 12 that is the holding portion of the first cutting unit 1 is formed such that the opening in which the blade 11 is formed is inclined from one end to the other end. By making the 1st cutting | disconnection unit 1 into such a structure, the collection instrument 120 can reduce the resistance at the time of cut | disconnecting hard biological tissues, such as the said skin tissue, by the collection instrument 120, for example. For this reason, the sampling device 120 can further reduce the destruction of the tissue structure of the living tissue caused by an excessive force during cutting. An intersection angle α between the inclination of the opening (two-dot chain line L3 in FIG. 3) and the cylinder 12 is, for example, 15 to 30 °. Further, in the sampling instrument 120, the support portion 22 of the second cutting unit 2 is a convex portion having a reverse tapered shape from the side surface toward the opening end side of the tube 12 on the opening end side of the tube 12. It is formed as (protruding part). The angle β of the convex portion (intersection angle β between the two-dot chain lines L4 and L5 in FIG. 3E) is, for example, 90 to 120 °. Except for these points, the sampling instrument 120 has the same configuration as the sampling instrument 100, and the description thereof can be used.

As shown in FIG. 4, the sampling instrument 130 has a first cutting unit 1 and a second cutting unit 2. In the sampling instrument 130, the cylinder 12 that is the holding part of the first cutting unit 1 has an opening in which the blade 11 is formed, and both side surfaces thereof are formed as concave parts having a reverse arc shape with respect to the living tissue. Yes. And in the opening part of the pipe | tube 12, the junction part of two recessed parts forms the protrusion part. Thereby, when cutting | disconnecting hard biological tissues, such as the said skin tissue, by the extractor 130, the resistance at the time of a cutting | disconnection start can be reduced. Moreover, in the collection tool 130, the support part 22 of the 2nd cutting | disconnection unit 2 is the same as the support part 22 of the collection tool 120, The description can be used. Except for these points, the sampling instrument 130 has the same configuration as the sampling instrument 100, and the description thereof can be used.

As shown in FIG. 5, the sampling instrument 140 has a first cutting unit 1 and a second cutting unit 2. In the collection tool 140, the support portion 22 of the second cutting unit 2 has a reduced length in the axial direction of the cylinder 12. Further, the second cutting unit 2 has contact surfaces with the two first cutting units 1, that is, the upper and lower surfaces of the blade 21 and the support portion 22 shown in FIG. It is integrated with the first cutting unit 1 by joining and fixing to the inner peripheral surface. By immobilizing the second cutting unit 2 in the first cutting unit 1, the collection tool 140 can easily transmit, for example, a force when rotating the collection tool 140 to the blade 11 and the blade 21. For this reason, by adopting such a configuration, the sampling instrument 140 can easily collect the cut tissue even when the biological tissue to be collected is present at a deep position from the surface. Except for these points, the sampling instrument 140 has the same configuration as the sampling instrument 100, and the description thereof can be used.

As shown in FIG. 6, the sampling instrument 150 includes a first cutting unit 1 and a second cutting unit 2. In the collection tool 150, the length of the support portion 22 of the second cutting unit 2 in the axial direction of the cylinder 12 is shortened. Further, the second cutting unit 2 has first contact surfaces with the three first cutting units 1, that is, the upper end, the lower right end, and the lower left end of the blade 21 and the support portion 22 shown in FIG. It is integrated with the first cutting unit 1 by joining and fixing to the inner peripheral surface of the cutting unit 1. By immobilizing the second cutting unit 2 in the first cutting unit 1, the collection tool 150 can easily transmit, for example, a force when rotating the collection tool 150 to the blade 11 and the blade 21. For this reason, by adopting such a configuration, the sampling instrument 150 can easily collect the cut tissue even when the biological tissue to be collected exists at a deep position from the surface. In the sampling instrument 150, three blades 21 of the second cutting unit 2 are arranged from the center of the cross section perpendicular to the axial direction of the cylinder 12 toward the cylinder 12. Further, the blades 21 are arranged so that the crossing angles of the blades are substantially equal angles (about 120 °). By adopting such a configuration, for example, the sampling instrument 150 can reduce the rotation angle when rotating the second cutting unit 2, so that the tissue structure of the living tissue when cutting by the second cutting unit 2 can be reduced. Destruction can be further reduced. Except for these points, the sampling instrument 150 has the same configuration as the sampling instrument 100, and the description thereof can be used.

As shown in FIG. 7, the sampling instrument 160 has a first cutting unit 1 and a second cutting unit 2. In the sampling instrument 160, the support portion 22 of the second cutting unit 2 has a reduced length in the axial direction of the cylinder 12. Further, the second cutting unit 2 has first contact surfaces with the four first cutting units 1, that is, the upper end, right end, lower end, and left end surfaces of the blade 21 and the support portion 22 shown in FIG. It is integrated with the first cutting unit 1 by joining and fixing to the inner peripheral surface of the cutting unit 1. By immobilizing the second cutting unit 2 in the first cutting unit 1, the sampling instrument 160 can easily transmit, for example, a force when rotating the sampling instrument 160 to the blade 11 and the blade 21. Therefore, by adopting such a configuration, the sampling instrument 160 can easily collect the cut tissue even when the biological tissue to be collected is present at a deep position from the surface. Further, in the sampling instrument 160, four blades 21 of the second cutting unit 2 are arranged from the center of the cross section perpendicular to the axial direction of the cylinder 12 toward the cylinder 12. Further, the blades 21 are arranged so that the intersecting angles of the blades are substantially equal angles (about 90 °). By adopting such a configuration, for example, the sampling instrument 160 can reduce the rotation angle when rotating the second cutting unit 2, so that the tissue structure of the living tissue when cutting by the second cutting unit 2 Can be further reduced. Except for these points, the sampling instrument 160 has the same configuration as the sampling instrument 100, and the description thereof can be used.

As shown in FIG. 8, the sampling instrument 170 includes a first cutting unit 1 and a second cutting unit 2. In the sampling instrument 170, the second cutting unit 2 does not have the support portion 22, but has only the linear blade 21. Thereby, when cutting | disconnecting hard biological tissues, such as the said skin tissue, with the extractor 170, the resistance at the time of a cutting | disconnection start can be reduced. Further, in the second cutting unit 2, the contact portions with the two first cutting units 1, that is, the upper end and the left end of the blade 21 shown in FIG. 8B are joined to the inner peripheral surface of the first cutting unit 1. By being fixed, it is integrated with the first cutting unit 1. By immobilizing the second cutting unit 2 in the first cutting unit 1, the collection tool 170 can easily transmit, for example, a force when rotating the collection tool 170 to the blade 11 and the blade 21. For this reason, by adopting such a configuration, the sampling device 170 can easily collect the cut tissue even when the biological tissue to be collected is present at a deep position from the surface. Except for these points, the sampling instrument 170 has the same configuration as the sampling instrument 100, and the description thereof can be used.

The collection tool 100 may have, for example, an attachment unit for attachment to the collection device of the present invention described later. The attachment unit is disposed, for example, on the side opposite to the blade 11 in the cylinder 12 of the first cutting unit 1. For the mounting unit, the description of the mounting unit in the collection device described later can be used. The attachment unit disposed in the first cutting unit 1 may be, for example, an attachment tool described later.

The sampling instrument 100 may include, for example, a closing unit that closes the opening of the first cutting unit 1 so as to be openable and closable. The term “closed so as to be openable / closable” means, for example, that the closing unit can close the opening of the sampling device 100 and can open and close the opening in the state where it is arranged in the sampling device 100. To do. The closing unit is disposed, for example, when the first cutting unit 1 has an opening. The opening of the first cutting unit 1 in which the closing unit is arranged excludes, for example, the opening in which the blade 11 in the first cutting unit 1 is formed, that is, of the two openings in the first cutting unit 1 It is preferable that the opening is not formed with the blade 11. The closing unit may be anything that can close the opening so that it can be opened and closed, for example, a sheet that closes the outside of the opening, a closing member such as rubber that closes the inside of the opening, a combination of these, etc. can give. Examples of the sheet include a mesh. The combination is not particularly limited, and for example, a part of the opening is closed by the closing member, and the remaining part of the opening is closed by the sheet. The collection tool 100 includes the blocking unit. For example, when the collection tool 100 is moved in the direction opposite to the arrow X direction after being cut by the first cutting unit 1 and the second cutting unit 2, the first cutting unit 100 A negative pressure is generated in the internal space of 1, and the cut tissue in the first cutting unit 1 can be prevented from dropping (detaching) from the collection tool 100. For this reason, according to the collection | collection instrument 100 which has the said obstruction | occlusion unit, the said cutting | disconnection tissue can be collect | recovered more simply. For example, the closing unit may be provided as a separate member from the first cutting unit 1 or may be provided as the same member, that is, may be formed integrally with the first cutting unit 1.

In the collection tool 100, for example, the first cutting unit 1 may include a regulating unit that regulates the detachment of the cut tissue held in the cylinder 12 that is a holding unit. For example, when the regulating unit 100 leads the sampling device 100 out of the living tissue, negative pressure is generated in the internal space of the first cutting unit 1, and the cut tissue in the first cutting unit 1 is removed from the cylinder 12. Prevent detachment. As a specific example, the restricting unit is, for example, a valve such as a one-way valve (check valve). When the collection instrument 100 includes the blocking unit, the blocking unit may also serve as the restricting unit. As for the arrangement | positioning location of the control part in the 1st cutting unit 1, the opening part in which the blade 11 is not formed, the inside of the cylinder 12, etc. are mention | raise | lifted, for example. When the restricting portion is disposed inside the cylinder 12, the restricting portion is located at a position that does not come into contact with the obtained cut tissue when the living tissue is cut by the first cutting unit 1 and the second cutting unit 2. Preferably they are arranged.

The collection tool 100 may include a collection unit that can collect the cut tissue held in the cylinder 12 that is a holding unit of the first cutting unit 1 to the outside of the cylinder 12, for example. The collection unit includes, for example, suction means capable of sucking the inside of the cylinder 12 from an opening in which the blade 11 as the first cutting part is formed or an opening in which the blade 11 is not formed, and an opening in which the blade 11 is formed. Examples thereof include a pressurizing means that can pressurize the inside of the cylinder 12 from an opening in which no part or blade 11 is formed, an extruding means that contacts the cut tissue in the cylinder 12 and pushes the cut tissue. Examples of the extruding means include a rod-shaped member. The shape of the rod-shaped member can be appropriately designed according to the shape of the blade 11 and the cylinder 12, for example.

According to the collection device of the present invention, for example, the cut tissue can be collected by introducing the collection device into a biological tissue, rotating it within the biological tissue, and deriving from the biological tissue. For this reason, according to the extraction | collection instrument of this invention, in the formation of the said cutting | disconnection tissue, it can implement without performing the suction | attraction of the said biological tissue. Therefore, according to the collection device of the present invention, it is possible to collect a biological tissue with reduced destruction of the tissue structure.

<Biological tissue collection device>
As described above, the biological tissue collection device of the present invention includes the biological tissue collection device of the present invention, a moving unit, and a rotation unit. The moving unit moves the biological tissue collection device toward the biological tissue. The rotation unit can move forward and backward from the living tissue, and the rotation unit can rotate the second cutting unit. The collection device of the present invention is characterized in that the collection device is the collection device of the present invention, and other configurations and conditions are not particularly limited. The collection device of the present invention can use, for example, the description of the collection device of the present invention and the collection method of the present invention described later. The collection device of the present invention can be implemented without, for example, aspiration of the living tissue in forming the cut tissue. Therefore, according to the collection device of the present invention, it is possible to collect a biological tissue with reduced destruction of the tissue structure. Moreover, according to the collection device of the present invention, for example, the cut tissue can be collected from a living tissue having an arbitrary thickness.

The sampling device of the present invention can separate a part of the object from the object, for example. For this reason, the collection device of the present invention can also be referred to as a “biological tissue separation device”, for example. The object is not particularly limited, and examples thereof include solid cancers such as breast cancer and skin cancer. When the collection device of the present invention is used for separating the cancer, the collection device of the present invention can also be referred to as, for example, a “surgical device”. The collection device of the present invention can also be used as a biological tissue collection device in a medical instrument such as an endoscope. In this case, the collection device of the present invention can also be referred to as a “medical device”, for example.

The sampling device of the present invention will be described with reference to FIG. FIG. 9 is a schematic view showing an example of the collection device of the present invention. 9A is a perspective view, and FIG. 9B is an enlarged view of the collection tool 100 in FIG. In FIG. 9, the living tissue is arranged in the direction of the arrow. As shown in FIG. 9, the collection device 200 includes a collection device 100, a moving unit 3, a rotation unit 4, a rotation stop unit 5, and a mounting unit 6 as main components. The moving unit 3 includes a guide 31, a cover 32, a support tool 33, a gripping tool 34, and a spring member 35. The guide 31 has a groove 31 a into which the protrusion 5 a of the rotation stop unit 5 can be inserted and removed. The guide 31 is connected to the grip portion 34 at the connecting portion 36. The cover 32 accommodates the guide 31 so that the guide 31 can move inside. The cover 32 has a support 33 fixed to the end of the rotary unit 4. The support tool 33 is connected to the gripping tool 34 at the pivot portion 37 so that the gripping tool 34 can pivot. The spring member 35 has one end fixed to the support tool 33 and the other end fixed to the gripping tool 34. The spring member 35 elastically biases the gripping tool 34 in the direction of the arrow when the lower portion of the gripping tool 34 is moved so as to approach the support tool 33 from the connecting portion with the pivot portion 37. The rotation unit 4 is arranged on the opposite end side of the connecting portion with the mounting unit 6 in the guide 31 so that the guide 31 can rotate. The rotation stop unit 5 has a protruding portion 5a at one end thereof. The rotation stopper unit 5 has a protruding portion 5a at one end thereof disposed so as to be insertable into and removable from the groove 31a. Further, the other end of the rotation stopper unit 5 is fixed to the support 33. The attachment unit 6 includes a connection instrument 61 and an attachment instrument 62. The connection tool 61 is disposed on the end side of the collection tool 100 of the guide 31. The attachment device 62 is attached to one end of the collection device 100. The attachment device 62 is detachably disposed on the connection device 61. In FIG. 9A, the sampling instrument 100 to which the mounting instrument 62 is attached is disposed so as to protrude from the connecting instrument 61 in the direction of the arrow (the direction of the biological tissue). It may be accommodated inside the connection device 61. In this case, for example, when the biological tissue is collected, the collection device 100 is moved from the inside of the connection device 61 to the outside by the moving unit 3 and protrudes from the connection device 61 toward the biological tissue. Further, for example, after the biological tissue is collected, the collection device 100 is moved from the outside of the connection device 61 to the inside by the moving unit 3 and is accommodated in the connection device 61. In the sampling device 200, the rotation stop unit 5 and the attachment unit 6 are arbitrary configurations, and may or may not be present.

The collection of the biological tissue by the collection device 200 is performed as follows, for example. First, the sampling instrument 100 with the mounting instrument 62 disposed at one end is attached to the connection instrument 61 of the sampling apparatus 200. The blade 11 of the first cutting unit 1 of the sampling instrument 100 is arranged in the direction of the biological tissue to be cut (the arrow direction in FIG. 9A). Next, a lower part than the connection part with the pivot part 37 of the holding | gripping tool 34 of the collection device 200 is moved to the support tool 33 side. Then, since the gripping tool 34 is connected to the guide 31 at the connecting portion 36, the guide 31 moves in the arrow direction around the pivot portion 37. As a result, the first cutting unit 1 of the collection tool 100 is moved into the living tissue and then introduced into the living tissue, thereby cutting the cut tissue and forming a side surface of the cut tissue (first). 1 cutting step). Next, the protrusion 5a of the rotation stop unit 5 disposed in the groove 31a of the guide 31 is extracted from the groove 31a. Thereby, the guide 31 can be rotated by the rotation unit 4. And if the rotation unit 4 is rotated, force will be conducted through the guide 31 and the attachment unit 6, and the sampling instrument 100 will rotate in the circumferential direction. Thereby, the 2nd cutting unit 2 cut | disconnects the biological tissue cut | disconnected by the 1st cutting unit 1, and forms the bottom face of the said cut | disconnected tissue (2nd cutting process). Further, when the gripping tool 34 is released, the gripping tool 34 is moved in the direction of the arrow by the spring member 35. Then, the guide 31 connected to the gripping tool 34 and the connecting portion 36 moves in the direction opposite to the arrow direction around the pivot portion 37. As a result, the sampling device 100 is led out of the living tissue, and the cut tissue in the sampling device 100 can be recovered (recovery step).

In the sampling device 200, the moving unit 3 includes a guide 31, a cover 32, a support tool 33, a gripping tool 34, a spring member 35, a connecting portion 36, and a pivoting portion 37, all of which have an arbitrary configuration. May or may not be. The moving unit 3 can employ any configuration that allows the collection instrument 100 to advance toward and away from the living tissue. By adopting the above configuration, the moving unit 3 can simplify introduction of the collection tool 100 into the living tissue, cutting of the living tissue, and derivation of the cut tissue, for example. Although the movement unit 3 controls the movement of the collection tool 100 via the attachment unit 6, that is, indirectly, the movement unit 3 may directly control the movement. As a specific example, the mobile unit 3 may be directly connected to the collection tool 100, for example. The moving unit 3 may include, for example, a confirmation unit that can confirm the moving distance of the sampling device 100 by the moving unit 3 and an adjusting unit that can adjust the moving distance of the sampling device 100. Examples of the confirmation unit include a scale. The confirmation unit is provided on the cover 32, for example. For example, the adjustment unit can adjust the movement of the sampling device 100 in one or more stages. As a specific example, for example, the adjusting unit moves the gripping tool 34 to the support tool 33, and stops the movement of the gripping tool 34 when the collection tool 100 moves a predetermined distance.

In the collection device 200, the rotation unit 4 controls the rotation of the second cutting unit 2 of the collection tool 100 via the guide 31 and the mounting unit 6, that is, indirectly, but directly controls the rotation. May be. As a specific example, the rotation unit 4 may be directly connected to the second cutting unit 2 of the collection tool 100, for example. Moreover, the rotation unit 4 can employ | adopt the arbitrary structures which can rotate the 2nd cutting unit 2 of the collection instrument 100. FIG. In the collection device 200, the rotation unit 4 rotates the collection tool 100, but the rotation unit 4 may rotate only the second cutting unit 2.

In the collection device 200, the rotation stopper unit 5 has an arbitrary configuration as described above, and may or may not be present. The anti-rotation unit 5 can employ any configuration that can inhibit the rotation of the sampling device 100 by the rotation unit 4. The rotation stop unit 5 is configured to be able to release the inhibition of rotation of the rotation unit 4, for example. Although the rotation prevention unit 5 arrange | positions the protrusion part 5a in the groove | channel 31a of the guide 31 so that insertion or removal is possible, the rotation of the collection instrument 100 by the rotation unit 4 is indirectly inhibited, but directly inhibited. Also good. As a specific example, the rotation stopper unit 5 is directly connected to the rotation unit 4, for example.

In the collection device 200, the mounting unit 6 has an arbitrary configuration as described above, and may or may not be present. The attachment unit 6 can employ any configuration that allows the collection tool 100 to be detachably attached. As a specific example, the attachment unit 6 can use, for example, a removable fixture such as a mechanical chuck, a scroll chuck, an independent chuck, a drill chuck, a collet chuck, a magnet chuck, a vacuum chuck, and a chuck device. As a specific example, the connection tool 61 of the mounting unit 6 can use a collet, for example, and the mounting tool 62 can use a vacuum chuck. For example, the collection device 200 includes the attachment unit 6 so that the collection device 100 on which the attachment device 62 is arranged can be replaced. Therefore, it is possible to prevent the blood or the like attached when different biological tissues or organisms are collected.

The collection device 200 may include, for example, a closing unit that closes the opening of the collection tool 100 so that the opening can be opened and closed. For the closure unit, for example, the description of the closure unit in the sampling device 100 can be cited. The closing unit is disposed, for example, at the end of the moving unit 3 on the side of the collection instrument 100, the attachment unit 6, and the like.

The collection device 200 includes the collection device 100 as the collection device of the present invention, but may include other collection devices, for example, the collection devices 110 to 170.

According to the collection apparatus of the present invention, for example, the cut tissue can be collected by introducing the collection device into a biological tissue, rotating the biological instrument in the biological tissue, and deriving from the biological tissue. For this reason, according to the collection device of the present invention, the cut tissue can be formed without aspiration of the living tissue. Therefore, according to the collection device of the present invention, it is possible to collect a biological tissue with reduced destruction of the tissue structure.

<How to collect biological tissue>
As described above, the biological tissue collection method of the present invention introduces the biological tissue collection device of the present invention into the biological tissue, and cuts the biological tissue with the first cutting unit to form the side surface of the cut tissue. A first cutting step; a second cutting step in which the biological tissue collection device is rotated and the biological tissue is cut by the second cutting unit to form a bottom surface of the cut tissue; and the biological tissue collection device And a recovery step of recovering the cut tissue from the living tissue. The collection method of the present invention is characterized in that the collection device of the present invention is used for collecting the biological tissue, and other steps and conditions are not particularly limited. For the collection method of the present invention, for example, the description of the collection instrument and collection apparatus of the present invention can be used.

The sampling method of the present invention can separate a part of an object from the object, for example. For this reason, the collection method of the present invention can also be referred to as a “biological tissue separation method”, for example. The object is not particularly limited, and examples thereof include solid cancers such as breast cancer and skin cancer. When the collection method of the present invention is used for the separation of the cancer, the method apparatus of the present invention can also be referred to as, for example, a “surgical method”.

The first cutting step is a step of introducing the biological tissue collection device of the present invention into a biological tissue and cutting the biological tissue with the first cutting unit to form a side surface of the cut tissue. Specifically, first, the collection tool of the present invention is brought into contact with the surface of a biological tissue, and the collection tool is moved toward the biological tissue (collection region) to be collected in the biological tissue. When the biological tissue is present in the living body, the sampling device may be implemented by, for example, bringing the sampling device into contact with the surface of the living body and moving the sampling device toward the sampling region in the living body. The collection device may be brought into contact with the surface of the biological tissue or the biological surface at an arbitrary angle, for example. For example, since the resistance of the first cutting unit during cutting of the living tissue can be reduced, the collection tool is preferably brought into contact with the surface of the living tissue or the surface of the living body in a substantially orthogonal direction. Thereby, the said collection instrument can reduce destruction of the tissue structure of the biological tissue (cutting | disconnecting tissue) produced by the excessive force at the time of cutting | disconnection, for example. Next, the sampling instrument is moved, for example, so that the sampling area is located inside the sampling instrument, specifically, inside the first cutting unit. Thereby, the said sampling instrument forms the cut surface around the said collection area | region which hits the said side surface, for example. When the second cutting unit is detachably disposed in the first cutting unit, the second cutting unit may be introduced after the introduction of the first cutting unit. For example, since the collection instrument can reduce resistance during cutting of the biological tissue, it is preferable to introduce the collection instrument into the biological tissue in a state where the second cutting unit is disposed in the first cutting unit. . Thereby, the said collection instrument can reduce destruction of the tissue structure of the biological tissue (cutting | disconnecting tissue) produced by the excessive force at the time of cutting | disconnection, for example.

In the second cutting step, the biological tissue collecting device is rotated, and the biological tissue is cut by the second cutting unit to form the bottom surface of the cut tissue. When the second cutting unit is detachably disposed in the first cutting unit, only the second cutting unit, or the first cutting unit and the second cutting unit are rotated. On the other hand, when the second cutting unit is fixed in the first cutting unit, or when the second cutting unit and the first cutting unit are integrated, the first cutting unit and the second cutting unit The unit is rotated. As a result, the second cutting unit cuts the living tissue that contacts when rotating, and forms the bottom surface. More specifically, the second cutting unit cuts the distal end of the collection region located inside the first cutting unit to form the bottom surface. Further, since the bottom surface intersects the side surface, for example, the bottom surface and the side surface are connected. For this reason, the said cut | disconnected tissue is located in the said 1st cutting unit, for example, and isolate | separates from the surrounding biological tissue.

The first cutting step and the second cutting step may be performed at the same time or separately, for example. In the former case, in the first cutting step and the second cutting step, the side surface and the bottom surface are formed simultaneously, for example. In the latter case, the sampling method of the present invention may perform the second cutting step after the first cutting step, or the first cutting step after the second cutting step. You may implement. In the collection method of the present invention, the first cutting step and the second cutting step can reduce the destruction of the tissue structure of the living tissue (cutting tissue), for example. It is preferable to carry out the second cutting step. In this case, in the second cutting step, the biological tissue collection tool is rotated, and the biological tissue cut by the first cutting unit is cut by the second cutting unit to form the bottom surface of the cut tissue. .

In the collecting step, the sampling device is led out of the living tissue, and the cut tissue is collected. The cut tissue is, for example, located in the first cutting unit and separated from the surrounding biological tissue. For this reason, the cutting | disconnection structure | tissue in the said 1st cutting unit can be derived | led-out by deriving | leading out the said collection instrument containing the said 1st cutting unit out of the said biological tissue or the said biological body. Therefore, the collection device can collect the cut tissue. The moving direction of the sampling device at the time of the derivation is, for example, opposite to the moving direction when the sampling device is introduced. In the case where the collection device includes a closing unit, the recovery step may be performed after the opening of the collection device is closed by the closing unit. Thereby, at the time of derivation | leading-out of the said collection instrument, it can suppress that the said cutting | disconnection structure | tissue falls off (detach | detaches) from the said collection instrument, for example. The collection step may further include, for example, a step of collecting the cut tissue by leading the cut tissue in the collection tool out of the collection device. The cutting tissue can be derived using, for example, the recovery unit.

In the collection method of the present invention, the collection device of the present invention including the collection device may be used as the collection device. In this case, in the first cutting step, the biological tissue collection tool is introduced into the biological tissue by the moving unit, and the biological tissue is cut by the first cutting unit to form a side surface of the cut tissue. Further, in the second cutting step, the sampling unit is rotated by the rotating unit, the biological tissue cut by the first cutting unit is cut by the second cutting unit, and the bottom surface of the cut tissue is removed. Form. In the recovery step, the biological tissue collection device is led out of the biological tissue by the moving unit, and the cut tissue is recovered. Except for this point, the collection device of the present invention can collect the cut tissue as in the case of using the collection instrument of the present invention.

The embodiments of the present invention have been described above by way of example, but the scope of the present invention is not limited to these embodiments, and can be changed or modified according to the purpose within the scope of the claims. is there.

<Appendix>
Some or all of the above embodiments and examples can be described as the following supplementary notes, but are not limited thereto.
(Appendix 1)
Including a first cutting unit and a second cutting unit;
The first cutting unit cuts the living tissue to form a side surface of the cut tissue;
The second cutting unit, when rotating, cuts the living tissue to form a bottom surface of the cut tissue,
The second cutting unit is disposed in the first cutting unit;
The biological tissue collection device, wherein the bottom surface is a surface direction intersecting with the side surface.
(Appendix 2)
The sampling instrument according to appendix 1, wherein the second cutting unit is inscribed in the first cutting unit.
(Appendix 3)
The collection instrument according to appendix 1 or 2, wherein the second cutting unit includes a second cutting unit that cuts the living tissue and a support unit that supports the second cutting unit.
(Appendix 4)
The collection instrument according to appendix 3, wherein the second cutting unit is inscribed in the first cutting unit.
(Appendix 5)
The collection instrument according to appendix 3 or 4, wherein the second cutting section is arranged so as to cross the cross section in a cross section with respect to the axial direction of the first cutting unit.
(Appendix 6)
The first cutting unit includes a first cutting unit that cuts the living tissue, and a holding unit that holds the cut tissue,
The sampling instrument according to any one of appendices 1 to 5, wherein the first cutting part is disposed at one end of the holding part.
(Appendix 7)
The holding portion is a cylinder,
The sampling instrument according to appendix 6, wherein the first cutting part is disposed in an opening of the cylinder.
(Appendix 8)
The collection instrument according to appendix 6 or 7, wherein the first cutting unit includes a regulating unit that regulates detachment of the cut tissue held by the holding unit.
(Appendix 9)
The sampling instrument according to any one of appendices 1 to 8, wherein the second cutting unit is detachably disposed on the first cutting unit.
(Appendix 10)
The sampling instrument according to any one of appendices 1 to 8, wherein the second cutting unit is fixed to the first cutting unit.
(Appendix 11)
Including the biological tissue collection device according to any one of appendices 1 to 10, a moving unit, and a rotating unit;
The moving unit is capable of moving the biological tissue collection device forward and backward from the biological tissue;
The living tissue sampling apparatus, wherein the rotating unit is capable of rotating the second cutting unit.
(Appendix 12)
Including an occlusion unit,
The collection device according to appendix 11, wherein the closing unit closes an opening of the biological tissue collection device so as to be openable and closable.
(Appendix 13)
Including anti-rotation unit,
The collection device according to appendix 11 or 12, wherein the rotation stop unit inhibits rotation of the biological tissue collection device by the rotation unit.
(Appendix 14)
Including mounting unit,
The collection device according to any one of appendices 11 to 13, wherein the attachment unit detachably attaches the biological tissue collection device.
(Appendix 15)
A first cutting step of introducing the biological tissue collection device according to any one of appendices 1 to 10 into the biological tissue, and cutting the biological tissue with the first cutting unit to form a side surface of the cut tissue;
A second cutting step of rotating the biological tissue collection device and cutting the biological tissue by the second cutting unit to form a bottom surface of the cut tissue;
A method of collecting a biological tissue, comprising: a recovery step of extracting the biological tissue collection device out of the biological tissue and collecting the cut tissue.
(Appendix 16)
The biological tissue collection device is the biological tissue collection device according to any one of appendices 11 to 14, including the biological tissue collection device according to any one of appendices 1 to 10,
In the first cutting step, the moving unit introduces the living tissue collection device into the living tissue, and cuts the living tissue by the first cutting unit to form a side surface of the cutting tissue,
In the second cutting step, the biological tissue collection instrument is rotated by the rotating unit, and the biological tissue is cut by the second cutting unit to form a bottom surface of the cut tissue,
The collection method according to appendix 15, wherein, in the collection step, the biological tissue collection device is led out of the biological tissue and the cut tissue is collected by the moving unit.
(Appendix 17)
Appendix 15 or 16 wherein the biological tissue is at least one selected from the group consisting of cartilage, membrane tissue, skin tissue, subcutaneous tissue, vascular tissue, hormone-producing tissue, nerve, tendon, liver tissue, fat and muscle. The collection method described.

This application claims priority based on Japanese Patent Application No. 2018-056797 filed on Mar. 23, 2018, the entire disclosure of which is incorporated herein.

As described above, according to the present invention, for example, the cut tissue can be collected by introducing the sampling device into a biological tissue, rotating the biological instrument in the biological tissue, and then extracting the sample from the biological tissue. Therefore, according to the present invention, the cut tissue can be formed without aspiration of the living tissue. Therefore, according to the present invention, it is possible to collect a biological tissue with reduced destruction of the tissue structure. Therefore, the present invention is extremely useful, for example, in the medical field.

DESCRIPTION OF SYMBOLS 1 1st cutting unit 11, 21 Blade 12 Holding part 2 2nd cutting unit 22 Support part 3 Movement unit 31 Guide 32 Cover 33 Support tool 34 Holding tool 35 Spring member 36 Connection part 37 Pivoting part 4 Rotating unit 5 Anti-rotation unit 5a Protruding part 6 Mounting unit 61 Connecting tool 62 Mounting tool 100, 110, 120, 130, 140, 150, 160, 170 Sampling tool 200 Sampling device

Claims (17)

1. Including a first cutting unit and a second cutting unit;
   The first cutting unit cuts the living tissue to form a side surface of the cut tissue;
   The second cutting unit, when rotating, cuts the living tissue to form a bottom surface of the cut tissue,
   The second cutting unit is disposed in the first cutting unit;
   The biological tissue collection device, wherein the bottom surface is a surface direction intersecting with the side surface.
2. The sampling instrument according to claim 1, wherein the second cutting unit is inscribed in the first cutting unit.
3. The sampling instrument according to claim 1 or 2, wherein the second cutting unit includes a second cutting unit that cuts the living tissue and a support unit that supports the second cutting unit.
4. The sampling instrument according to claim 3, wherein the second cutting unit is inscribed in the first cutting unit.
5. The sampling instrument according to claim 3 or 4, wherein the second cutting section is arranged so as to cross the cross section in a cross section with respect to the axial direction of the first cutting unit.
6. The first cutting unit includes a first cutting unit that cuts the living tissue, and a holding unit that holds the cut tissue,
   The said 1st cutting part is a collection instrument as described in any one of Claim 1 to 5 arrange | positioned at the end of the said holding | maintenance part.
7. The holding portion is a cylinder,
   The sampling instrument according to claim 6, wherein the first cutting part is disposed in an opening of the cylinder.
8. The sampling instrument according to claim 6 or 7, wherein the first cutting unit includes a regulating unit that regulates detachment of the cut tissue held by the holding unit.
9. The collection instrument according to any one of claims 1 to 8, wherein the second cutting unit is detachably disposed on the first cutting unit.
10. The sampling instrument according to any one of claims 1 to 8, wherein the second cutting unit is fixed to the first cutting unit.
11. The biological tissue collection device according to any one of claims 1 to 10, a moving unit, and a rotating unit,
    The moving unit is capable of moving the biological tissue collection device forward and backward from the biological tissue;
    The living tissue sampling apparatus, wherein the rotating unit is capable of rotating the second cutting unit.
12. Including an occlusion unit,
    The collection device according to claim 11, wherein the closing unit closes an opening of the biological tissue collection device so as to be openable and closable.
13. Including anti-rotation unit,
    The collection device according to claim 11 or 12, wherein the rotation stop unit inhibits rotation of the biological tissue collection device by the rotation unit.
14. Including mounting unit,
    The collection device according to any one of claims 11 to 13, wherein the attachment unit detachably attaches the biological tissue collection instrument.
15. A first cutting step of introducing the biological tissue collection device according to any one of claims 1 to 10 into a biological tissue, and cutting the biological tissue with the first cutting unit to form a side surface of the cut tissue. ,
    A second cutting step of rotating the biological tissue collection device and cutting the biological tissue to form a bottom surface of the cut tissue by the second cutting unit;
    A method of collecting a biological tissue, comprising: a recovery step of extracting the biological tissue collection device out of the biological tissue and collecting the cut tissue.
16. The biological tissue collection device according to any one of claims 11 to 14, wherein the biological tissue collection device includes the biological tissue collection device according to any one of claims 1 to 10.
    In the first cutting step, the moving unit introduces the living tissue collection device into the living tissue, and cuts the living tissue by the first cutting unit to form a side surface of the cutting tissue,
    In the second cutting step, the biological tissue collection instrument is rotated by the rotating unit, and the biological tissue is cut by the second cutting unit to form a bottom surface of the cut tissue,
    The collection method according to claim 15, wherein, in the collection step, the biological tissue collection device is led out of the biological tissue and the cut tissue is collected by the moving unit.
17. The biological tissue is at least one selected from the group consisting of cartilage, membrane tissue, skin tissue, subcutaneous tissue, vascular tissue, hormone-producing tissue, nerve, tendon, liver tissue, fat and muscle, or The collection method according to 16.
    
    

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