WO2019179072A1 - 一种植物组培容器 - Google Patents

一种植物组培容器 Download PDF

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Publication number
WO2019179072A1
WO2019179072A1 PCT/CN2018/108183 CN2018108183W WO2019179072A1 WO 2019179072 A1 WO2019179072 A1 WO 2019179072A1 CN 2018108183 W CN2018108183 W CN 2018108183W WO 2019179072 A1 WO2019179072 A1 WO 2019179072A1
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WO
WIPO (PCT)
Prior art keywords
container
tissue culture
plant tissue
upper container
opening
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PCT/CN2018/108183
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English (en)
French (fr)
Inventor
李绍华
郑延海
李海滨
王雅缘
郑娜娜
巫杨文
Original Assignee
福建省中科生物股份有限公司
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Publication of WO2019179072A1 publication Critical patent/WO2019179072A1/zh

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029—Receptacles for seedlings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture

Definitions

  • the invention relates to the technical field of plant tissue culture, in particular to a plant tissue culture container capable of improving the inoculation efficiency and the utilization rate of tissue culture seedlings.
  • Tissue culture technique is to place living ex vivo organs (such as roots, stems, leaves, stem segments, protoplasts), tissues or cells in a medium under artificially created aseptic conditions and place them in a suitable environment. Techniques for continuous culture to obtain cells, tissues or individuals. This technology has been widely used in agricultural, biological and pharmaceutical research.
  • tissue culture containers used for plant tissue culture are mostly tissue culture bottles with high shrinkage or high bottle body.
  • tissue culture containers are often inconvenient for inoculation due to small bottle mouth or deep bottle body.
  • the inoculation efficiency is low.
  • the shrinkage bottle grows to a certain height, the shrinkage bottle will cause mutual crowding, and the shrinkage bottle will be subjected to large resistance when the tissue culture seedling is taken out, which is easy to cause mechanical damage and reduce the tissue culture seedling. Utilization.
  • the object of the present invention is at least to provide a plant tissue culture container which improves the inoculation efficiency and the utilization rate of tissue culture seedlings.
  • an embodiment of the present invention provides a plant tissue culture container comprising a lower container and an upper container, the lower container and the upper container each comprising a container body having a bottom closed and an upper opening;
  • the upper container is inverted on the lower container, and the openings of the two are detachably connected.
  • the container body of the upper container is provided with a venting hole, and the venting hole is covered by a gas permeable membrane.
  • the venting opening is a single through hole or a mesh opening.
  • the vent hole is opened at the bottom of the upper container, and the inner side of the bottom of the upper container is provided with a mounting hole extending from the periphery of the vent hole toward the opening of the upper container, and the gas permeable membrane is installed by Plastic screws or plastic rivets into the mounting hole section are pressed over the venting holes.
  • the lower container opening and the upper container opening are screwed or snap-fitted.
  • the lower container and the upper container are wide mouth containers.
  • the lower container and/or the upper container are of a coarse and fine structure.
  • the height of the lower container is less than or equal to twice the height of the upper container.
  • the above technical solution provided by the embodiment of the present invention divides the tissue culture container into two upper and lower parts which are inverted together, so that the bottom depth of the lower container is moderate, which facilitates the inoculation operation and the removal of the tissue culture seedling, thereby improving the work efficiency and the group. Peony seedling utilization.
  • Figure 1 is a schematic view showing the structure of a plant tissue culture container in one embodiment of the present invention.
  • Figure 1 shows an embodiment of a plant tissue culture container of the present invention.
  • the plant tissue culture container comprises two parts, a lower container 1 and an upper container 2.
  • the lower container 1 and the upper container 2 each include a container body with a bottom closed and an upper opening.
  • the upper container 2 is inverted on the lower container 1, and the openings of the two are detachably connected together, for example, as shown in FIG. Threaded connection.
  • an external thread is provided in the opening of the lower container 1
  • an internal thread that is fitted to the external thread of the lower container is provided in the opening of the upper container 2, or vice versa.
  • the threaded connection described above may also be replaced by a snap connection or the like in other embodiments.
  • a venting hole (not shown) is formed in the container body of the upper container 2.
  • the bottom of the upper container 2 is opened, and the venting hole may be a single through hole or a mesh opening.
  • the venting holes are covered by the gas permeable membrane 3 for aeration and filtration.
  • the gas permeable membrane 3 may cover the outer side of the upper container 2 or may cover the inner side of the upper container 2 as shown in FIG.
  • the inside of the upper container 2 can be installed on the inner side of the upper container 2: the inner side of the bottom of the upper container 2 is provided with a mounting hole extending from the periphery of the vent hole to the opening direction of the container 2, and the inner wall of the mounting hole is provided with an internal thread.
  • the gas permeable membrane 3 is placed in the mounting hole section, and the plastic screw 4 is screwed into the mounting hole section to cover the gas permeable membrane by squeezing the gas permeable membrane 3.
  • the plastic screw can also be replaced with a plastic rivet, in which case the inner wall of the mounting hole section does not have to be internally threaded.
  • the tissue culture container is divided into upper and lower parts which are inverted together, so that the lower container is lower in height than the tissue culture container in the prior art, which is convenient for inoculation operation and cleaning, and is convenient for taking out the tissue culture seedling and reducing the physics. Damage, improve the utilization rate of tissue culture seedlings.
  • the upper and lower containers can be up and down, high, short, or high. In the case of a lower high and upper short form, it is preferred that the height of the lower container is less than or equal to twice the height of the upper container.
  • the lower container 1 and the upper container 2 in this embodiment are both wide-mouth containers, which further improves the inoculation efficiency and the utilization rate of the tissue culture seedlings. Moreover, the lower container 1 and the upper container 2 are both thick and thin, which are convenient for folding storage and reduce space occupation.
  • the tissue culture container is preferably made of a material having high light transmittance and high temperature resistance, which improves the utilization of light energy and satisfies the requirements of high temperature sterilization of 120 to 130.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

一种植物组培容器，该组培容器包括下容器（1）和上容器（2），下容器（1）和上容器（2）均包括底部封闭、上部开口的容器本体；使用时，上容器（2）倒扣在下容器（1）上，二者的开口部可拆卸地连接。通过将组培容器分为倒扣在一起的上下两部分，使得下容器（1）的底部深度适中，便于接种操作和组培苗的取出，提高了工作效率和组培苗利用率。

Description

一种植物组培容器 技术领域
本发明涉及植物组织培养技术领域，具体涉及一种能够提高接种效率和组培苗利用率的植物组培容器。
背景技术
组织培养技术是在人为创造的无菌条件下将活的离体器官(如根、茎、叶、茎段、原生质体)、组织或细胞置于培养基内，并放在适宜的环境中，进行连续培养以获得细胞、组织或个体的技术。这种技术已广泛应用于农业和生物、医药的研究。
传统的用于植物组织培养的组培容器多为缩口或瓶身较高的组培瓶，这种组培容器往往由于瓶口较小，或瓶身较深，造成外植体接种不方便，接种效率低。另一方面，缩口瓶在组培苗长到一定的高度时，会造成相互拥挤的现象，而且缩口瓶在组培苗取出时受到的阻力较大，容易造成机械损伤，降低组培苗的利用率。
发明内容
鉴于以上所述现有技术的缺点，本发明的目的至少在于提供一种提高接种效率和组培苗利用率的植物组培容器。
为实现上述目的及其他相关目的，本发明的一个实施方式提供一种植物组培容器，包括下容器和上容器，所述下容器和上容器均包括底部封闭、上部开口的容器本体；使用时，所述上容器倒扣在下容器上，二者的开口部可拆卸地连接。
在一个实施方式中，所述上容器的容器本体上开设有透气孔，所述透气孔由透气膜覆盖。
在一个实施方式中，所述透气孔为单一通孔或网状开口。
在一个实施方式中，所述透气孔开设在所述上容器的底部，所述上容器的底部内侧设置从透气孔周围向所述上容器开口部延伸的安装孔段，所述透气膜通过安装到所述安装孔段内的塑料螺钉或塑料铆钉挤压覆盖在所述透气孔上。
在一个实施方式中，所述下容器开口部和所述上容器开口部螺纹连接或卡扣连接。
在一个实施方式中，所述下容器和所述上容器为广口容器。
在一个实施方式中，所述下容器和/或所述上容器为上粗下细的结构。
在一个实施方式中，所述下容器的高度小于或等于所述上容器的高度的2倍。
本发明实施方式提供的上述技术方案，通过将组培容器分为倒扣在一起的上下两部分， 使得下容器的底部深度适中，便于接种操作和组培苗的取出，提高了工作效率和组培苗利用率。
附图说明
图1显示为本发明一个实施例中植物组培容器的结构示意图。
元件标号说明
1       下容器
2       上容器
3       透气膜
4       塑料螺钉
具体实施方式
以下由特定的具体实施例说明本发明的实施方式，熟悉此技术的人士可由本说明书所揭露的内容轻易地了解本发明的其他优点及功效。
须知，本说明书附图中所绘示的结构、比例、大小等，均仅用以配合说明书所揭示的内容，以供熟悉此技术的人士了解与阅读，并非用以限定本发明可实施的限定条件，故不具技术上的实质意义，任何结构的修饰、比例关系的改变或大小的调整，在不影响本发明所能产生的功效及所能达成的目的下，均应仍落在本发明所揭示的技术内容所能涵盖的范围内。同时，本说明书中所引用的如“上”、“下”、“左”、“右”、“中间”及“一”等的用语，亦仅为便于叙述的明了，而非用以限定本发明可实施的范围，其相对关系的改变或调整，在无实质变更技术内容下，当亦视为本发明可实施的范畴。
图1示出本发明植物组培容器的一个实施例。本实施例中，植物组培容器包括下容器1和上容器2两部分。下容器1和上容器2均包括底部封闭、上部开口的容器本体，使用时，上容器2倒扣在下容器1上，二者的开口部可拆卸地连接在一起，例如通过图1所示的螺纹连接方式。具体来说，在下容器1开口部设置外螺纹，在上容器2的开口部设置与下容器外螺纹配合的内螺纹，反之亦可。上述螺纹连接在其他实施方式中也可由卡扣连接等方式替代。
上容器2的容器本体上开设透气孔(图中未示出)，本实施例中开设在上容器2的底部，透气孔可以是单一通孔，也可为网状开口。透气孔由透气膜3覆盖，用于通气和滤菌。透气膜3可覆盖于上容器2的外侧，也可如图1所示覆盖于上容器2的内侧。透气膜3在上容器2内侧的安装可采用如图1所示的结构：上容器2的底部内侧设置从透气孔周围向上容器2开口方向延伸的安装孔段，安装孔段内壁设置内螺纹，将透气膜3放入该安装孔段内，再将 塑料螺钉4旋入该安装孔段，从而通过挤压透气膜3，使其覆盖在透气孔上。在其他实施方式中也可将塑料螺钉替换为塑料铆钉，这种情况下，安装孔段内壁可不必设置内螺纹。
上述方案中，组培容器分为倒扣在一起的上下两部分，使得下容器相比现有技术中的组培容器降低了高度，便于接种操作和清洗，且便于组培苗取出，减少物理损伤，提高组培苗的利用率。上下容器可以上下等高、上高下短或下高上短。采用下高上短的形式时，优选下容器的高度小于或等于上容器高度的2倍。
如图1所示，本实施例中的下容器1和上容器2均为广口容器，进一步提高了接种效率和组培苗利用率。并且下容器1和上容器2均为上粗下细的结构，便于摞叠存放，减少空间占用。
为有利于组培苗植株的生长，组培容器优选采用高透光、耐高温的材质，提高光能利用率，并且满足耐120～130高温消毒的要求。
上述实施例仅例示性说明本发明的原理及其功效，而非用于限制本发明。任何熟悉此技术的人士皆可在不违背本发明的精神及范畴下，对上述实施例进行修饰或改变。因此，举凡所属技术领域中具有通常知识者在未脱离本发明所揭示的精神与技术思想下所完成的一切等效修饰或改变，仍应由本发明的权利要求所涵盖。

Claims (8)

  1. 一种植物组培容器，其特征在于：
    所述组培容器包括下容器和上容器，所述下容器和上容器均包括底部封闭、上部开口的容器本体；
    使用时，所述上容器倒扣在下容器上，二者的开口部可拆卸地连接。
  2. 根据权利要求1所述的植物组培容器，其特征在于：
    所述上容器的容器本体上开设有透气孔，所述透气孔由透气膜覆盖。
  3. 根据权利要求2所述的植物组培容器，其特征在于：
    所述透气孔为单一通孔或网状开口。
  4. 根据权利要求2所述的植物组培容器，其特征在于：
    所述透气孔开设在所述上容器的底部，所述上容器的底部内侧设置从透气孔周围向所述上容器开口部延伸的安装孔段，所述透气膜通过安装到所述安装孔段内的塑料螺钉或塑料铆钉挤压覆盖在所述透气孔上。
  5. 根据权利要求1所述的植物组培容器，其特征在于：
    所述下容器开口部和所述上容器开口部螺纹连接或卡扣连接。
  6. 根据权利要求1所述的植物组培容器，其特征在于：
    所述下容器和所述上容器为广口容器。
  7. 根据权利要求1所述的植物组培容器，其特征在于：
    所述下容器和/或所述上容器为上粗下细的结构。
  8. 根据权利要求1-7任一所述的植物组培容器，其特征在于：
    所述下容器的高度小于或等于所述上容器的高度的2倍。
PCT/CN2018/108183 2018-03-22 2018-09-28 一种植物组培容器 WO2019179072A1 (zh)

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108464239A (zh) * 2018-03-22 2018-08-31 福建省中科生物股份有限公司 一种植物组培容器
CN110800615A (zh) * 2019-12-13 2020-02-18 福建省中科生物股份有限公司 一种圆形植物组培盒子

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015527A1 (en) * 1989-06-13 1990-12-27 Agristar, Inc. Integument with breakable inner containers
CN201550489U (zh) * 2009-09-22 2010-08-18 北京农业生物技术研究中心 一种组织培养用容器
CN102835310A (zh) * 2012-05-12 2012-12-26 姜伟 一种双盘组合式植物组织培养容器
CN202799770U (zh) * 2012-07-25 2013-03-20 中国热带农业科学院热带生物技术研究所 一种组织培养容器
CN205727483U (zh) * 2016-06-13 2016-11-30 天津丰华裕隆农业发展有限公司 一种组合式组培瓶
CN206851647U (zh) * 2017-01-26 2018-01-09 欣新兰艺有限公司 植物培养盒结构
CN206895454U (zh) * 2017-05-10 2018-01-19 河南联源生物科技有限公司 一种新型石斛组培瓶
CN207011442U (zh) * 2017-07-25 2018-02-16 皖西学院 一种石斛组织培养瓶
CN108464239A (zh) * 2018-03-22 2018-08-31 福建省中科生物股份有限公司 一种植物组培容器

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015527A1 (en) * 1989-06-13 1990-12-27 Agristar, Inc. Integument with breakable inner containers
CN201550489U (zh) * 2009-09-22 2010-08-18 北京农业生物技术研究中心 一种组织培养用容器
CN102835310A (zh) * 2012-05-12 2012-12-26 姜伟 一种双盘组合式植物组织培养容器
CN202799770U (zh) * 2012-07-25 2013-03-20 中国热带农业科学院热带生物技术研究所 一种组织培养容器
CN205727483U (zh) * 2016-06-13 2016-11-30 天津丰华裕隆农业发展有限公司 一种组合式组培瓶
CN206851647U (zh) * 2017-01-26 2018-01-09 欣新兰艺有限公司 植物培养盒结构
CN206895454U (zh) * 2017-05-10 2018-01-19 河南联源生物科技有限公司 一种新型石斛组培瓶
CN207011442U (zh) * 2017-07-25 2018-02-16 皖西学院 一种石斛组织培养瓶
CN108464239A (zh) * 2018-03-22 2018-08-31 福建省中科生物股份有限公司 一种植物组培容器

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