WO2019141365A1 - Électro-bioréacteur et ses pièces pour réaliser des synthèses microbiennes stériles à usage unique ou réutilisable - Google Patents

Électro-bioréacteur et ses pièces pour réaliser des synthèses microbiennes stériles à usage unique ou réutilisable Download PDF

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Publication number
WO2019141365A1
WO2019141365A1 PCT/EP2018/051228 EP2018051228W WO2019141365A1 WO 2019141365 A1 WO2019141365 A1 WO 2019141365A1 EP 2018051228 W EP2018051228 W EP 2018051228W WO 2019141365 A1 WO2019141365 A1 WO 2019141365A1
Authority
WO
WIPO (PCT)
Prior art keywords
insert
lid
bioreactor
sterile
electrobioreactor
Prior art date
Application number
PCT/EP2018/051228
Other languages
German (de)
English (en)
Inventor
Steffi Hunger
Falk Harnisch
Carla Gimkiewicz
Vitaliy JEGOROW
Luis Filipe Morgado Rosa
Beate Strehlitz
Original Assignee
Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz filed Critical Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz
Priority to EP18701701.7A priority Critical patent/EP3740557A1/fr
Priority to PCT/EP2018/051228 priority patent/WO2019141365A1/fr
Publication of WO2019141365A1 publication Critical patent/WO2019141365A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements

Definitions

  • the present invention relates to an insert for a bioreactor vessel for forming an electrobioreactor for performing a sterile microbial electrosynthesis, an electrobioreactor with the aforementioned use, a kit comprising an electrobioreactor or its parts, a use of the aforementioned insert and the electro bioreactor.
  • Bioelectrosynthesis or electrobiosynthesis is divided into enzymatic electrosynthesis and microbial electrosynthesis.
  • the aim of microbial electrosynthesis is the linking of microbial synthesis power with the flow of electrical current.
  • both electrical current can be converted in the case of oxidation processes to anodes and also in the case of reductions at the cathode, and thus can be used as reactant.
  • microbial conversion is associated with the flow of electrical current.
  • the coupling is preferably carried out directly via extracellular electron transfer or indirectly via an abiotic electrochemical reaction for substrate or product conversion of microbial syntheses.
  • EET extracellular electron transfer
  • direct EET DEET
  • DEET direct electrode contact
  • MEET soluble molecules
  • the DEET can take place by means of membrane-bound redox molecules or via pili, which act like nanowires (nanowires).
  • the MEET may be via natural (endogenous) or process-added (exogenous) mediators.
  • microorganisms which are of course capable of EET or have been developed microbiologically or molecularly for it can be used.
  • the microbial electrosynthesis in comparison to classical electrochemical synthesis has numerous advantages such as potentially higher selectivities, yields, etc., but there are also advantages over enzymatic electrosyntheses, since in most cases only max. Two electrons can be transferred per reaction step and the catalyst is not self-regenerating, but this may be the case in microbial electrosynthesis.
  • sterile microbial electrosynthesis the following processes may be included:
  • Microorganisms and product formation with microorganisms, incl. The electrochemical fermentation control and
  • Reactors which are suitable for carrying out the microbial electrosynthesis are disclosed, for example, in the publications US 2013/0256149 A1, WO 201 1/062485 A2, WO 2010/117864 A1 and WO 00/03447 DE 10 2013 224 673 A1.
  • sterilizable bioreactors and sterile disposable bioreactors i. E. Reactors that are delivered in a sterile package and ready for immediate use are the drug of choice for research and development as well as for production.
  • Sterile bioreactors are well established for numerous applications in slightly different designs, for example with regard to the geometry of the bioreactor vessel.
  • an insert for a bioreactor vessel for forming an electrobioreactor for carrying out a sterile microbial electrosynthesis is provided.
  • This insert has a top, a bottom and a wall between the top and bottom running on.
  • the top of the insert is designed open and the wall is provided with at least one passage opening.
  • the at least one passage opening is closed by a preferably ion-selective membrane.
  • the insert according to the invention is suitable to be sterilized.
  • bioreactor is used if it is not equipped for microbial electrobiosynthesis. If the insert according to the invention is introduced into the bioreactor, this electrobioreactor is called.
  • This insert can be introduced into a bioreactor or into the bioreactor vessel of a bioreactor in order to subdivide the reaction chamber into two reaction chambers connected through the membrane and thus to provide an electrobioreactor with separation of anode chamber and cathode chamber.
  • the insert according to the invention can be sterilized, i. H.
  • the insert consists of sterilizable materials such as glass, ceramics or plastics, preferably plastics.
  • the sterilization can either be done by a user himself or the insert can be sterilized in a sterile package purchased, the use is suitable for re-sterilization or single use.
  • the membrane is an ion-conducting, preferably an ion-selective membrane.
  • the membrane is preferably an ion exchange membrane, preferably based on functionalized and cross-linked hydrocarbons.
  • a Nafion membrane or a ceramic membrane can be used.
  • Composite materials of ion exchange membranes and other plastics such as polyesters, polypropylenes and polyetheretherketone, (functionalized) ceramic materials and materials based on (functionalized) glasses and composite materials thereof can also be used according to the invention.
  • the membrane is preferably formed by casting, welding (eg gas welding, extrusion welding, heating element welding, RF welding, ultrasonic welding), gluing with adhesives based on acrylics, epoxies and the like or mechanical joining technologies (screwing, pressing, "snap") in the through hole of the For use with glass or ceramic inserts the membrane is already introduced or etched into the material directly during production. Crucial in the production process is that the resulting use is sterilizable.
  • the size and the number of at least one passage opening and thus the membrane (s) is chosen such that the internal resistance is minimized. Therefore, the ratio of the size of the electrodes used for the synthesis, which are respectively arranged in the two reaction chambers of the electrobioreactor, to the size and the number of the passage opening or the membrane (s) is expediently in the range from 1: 100 to 100: 1.
  • the through hole (s) may consist of one or more small through holes with round or polygonal shapes which may be evenly or irregularly distributed around the circumference of the insert.
  • the insert is cylindrically shaped, since this allows an optimal mixing of the media in the reaction chambers in the electrobioreactor.
  • the insert has a cover with which the opening of the insert or the open top can be closed.
  • the cover has at least one passage opening, which is preferably suitable and configured for receiving an electrode and a reference electrode.
  • Other ports may be provided for various purposes, as described below.
  • the guided during use of the insert through the through hole electrode protrudes into the insert into it.
  • Other devices guided by the lid such as a reference electrode, protrude into the reaction chamber formed by the insert.
  • the passage opening is guided through the lid like a through-hole.
  • it can also be provided a shoulder in the through hole, which can serve as a support for a corresponding counterpart of an electrode.
  • At least one sealing element for example an annular seal, can be provided in the at least one passage opening on or in the passage opening for the electrode in order to prevent influences from outside the electrobioreactor during the synthesis and to better ensure the sterility for the microbial bioelectrosynthesis.
  • a flange is preferably provided, on which the lid rests, it being possible for a seal to be arranged between the lid and the insert.
  • the insert is combined with a cover for a bioreactor or a bioreactor vessel.
  • the insert may be arranged in a passage opening of the lid, wherein the insert and the lid are separate components or may be integrally formed. When closing the electro bioreactor with the lid, the insert projects into the electro bioreactor.
  • means are provided to fix the insert in the passage opening of the cover for the Bioreaktorgefäß.
  • a sealing element for example an annular seal, is preferably provided between the cover for the bioreactor vessel and the lid of the insert.
  • the cover for the electrobioreactor is formed integrally with the insert.
  • the insert according to the invention can be made up as a sterile (once) product, which can also be used several times after renewed sterilization.
  • the insert according to the invention in the above-described embodiments can be arranged sterile in a sterile packaging on the inside.
  • the insert can also be designed to be open on the underside, wherein it must then be dimensioned in such a way that it rests on the bottom of the bioreactor vessel and is preferably sealed and fixed by suitable means.
  • the insert is preferably designed to be closed on the bottom side and, after introduction into the bioreactor vessel, is spaced from its bottom in order to receive suitable stirring means, such as magnetic stirrers, in the intermediate space.
  • an electrobioreactor for carrying out a sterile microbial electrosynthesis which has a bioreactor vessel open to one side (upper side) which has a previously described insert with lid and optionally a lid for closing the opening of the bioreactor vessel.
  • the insert is reversibly insertable into the bioreactor vessel and open to the same side as the bioreactor vessel, the insert being spaced from the interior wall of the bioreactor vessel, thereby forming a reaction chamber between the insert and the wall of the bioreactor vessel. Another reaction chamber is in use.
  • the bioreactor vessel is also suitable to be sterilized.
  • the insert has in the wall facing the inner wall of the reactor vessel a through-opening closed with a membrane, wherein the bioreactor vessel, the lid of the bioreactor vessel, the insert and / or the lid of the insert are suitable for being sterilized. Furthermore, reference is made to the above statements on the use.
  • the electrobioreactor and at least one, preferably all of the parts described above are made of an electrochemically inert material, which is also preferably autoclavable / steam sterilizable.
  • plastics for example polylactic acid
  • the cover of the bioreactor vessel and the lid of the insert for connecting the anode and cathode have suitable through openings, preferably with corresponding seals, wherein the through openings are configured to receive the electrodes in such a way that the anode / cathode is located in the insert, and the cathode / anode is located outside the insert in the bioreactor vessel.
  • passages or passage openings in the lid of the bioreactor vessel may be provided for introducing stirrers, probes, electrodes, reference electrodes, measuring probes, gassing or degassing, sampling and the like, wherein the passages or passage openings may be provided with seals.
  • the lid for the bioreactor vessel preferably has conventional attachment means and seals.
  • the bioreactor vessel at the top can also have a flange on which the lid rests and can be fixed by means known to those skilled in the art. Between the flange and the lid can also be arranged a seal.
  • the electrobioreactor or the bioreactor vessel and / or the lid of the bioreactor vessel and / or the insert and / or the lid of the insert sterile and in each case or together to arrange in a sterile on the inside packaging.
  • the invention furthermore relates to a kit for a previously described electrobioreactor, or a previously described bioreactor vessel and also an insert described above, optionally comprising a lid for the bioreactor vessel and / or a lid for use, wherein the components of the kit are sterile and sterile individually or together are packed.
  • Also claimed in accordance with the invention are the use of the bioreactor vessel, bioreactor vessel, electrobioreactor to perform sterile microbial electrosynthesis, and use of the resulting electrobioreactor to perform sterile microbial electrosynthesis.
  • the volume of the two reaction chambers of the electrobioreactor according to the invention is particularly preferably in the ratio 1: 1, but also ratios of 10: 1 to 1:10 are preferably used to carry out a sterile microbial electrosynthesis.
  • the electrodes used are anodes and cathodes as well as reference electrodes.
  • the anode or cathode is in the outer reaction chamber of the electro bioreactor and the respective other electrode in the inner reaction chamber, ie in use.
  • Both electrodes can each have a plurality of interconnected electronic conductors (electrodes), connected in parallel or in series; include.
  • Electrodes may have the same or different geometries and sizes and be made of the same or different materials. Suitable materials are metals and nonmetals. Suitable non-metals may be, for example, graphite and graphite modifications, eg carbon nanotubes or carbon nanoparticles, as well as all corresponding composite materials.
  • the metals used are, for example, gold, silver, zinc, gallium, copper, nickel, iron, platinum, titanium, etc., and their bi-, trinary and higher alloys. Furthermore, this includes electrode materials based on a functional surface coating of said materials on a substrate, including conductive substrates such as stainless steels or graphites.
  • the electrode specification may comprise all geometric shapes and modifications of said metals and nonmetals, in particular sheets, plates, films, rounds, tubes, sponges, scrims, fabrics, brushes, cylinders.
  • the reference electrode is preferably located in the outer electrobioreactor chamber, but may also be arranged in the inner chamber or in both chambers. As reference electrodes known in the art products are used.
  • the microbial electrosynthesis preferably takes place at direct current.
  • Figure 1 is a schematic representation of a microbial electrosynthesis on
  • FIG. 2 shows a perspective view of an electrobioreactor according to the invention with an insert for a bioreactor vessel
  • FIG. 1 shows by way of example a microbial electrosynthesis according to the prior art. It is a cathodic hydrogenation using microbial extracellular electron transfer.
  • reaction chambers 11 1, 112 each have an anode 113 and a cathode 1 14 is arranged, which are connected to a voltage source 115, wherein the cathode 114 is provided with a biofilm 116 of a bioelectrocactively active microorganism.
  • a substrate X is selectively reduced by taking up electrons from the cathode to the product XH 2 , while at the anode 1 13 H 2 0 to provide H + and electrons is oxidized, with electrons being delivered to the anode.
  • the membrane 1 10 Through the membrane 1 10, the corresponding ion transfer 117 takes place.
  • the resulting product XH 2 is removed from the reaction chamber 1 12, while substrate X is fed.
  • reaction substrate or the biofilm is not used as a growth substrate for the biofilm, but a separate growth substrate provides stable stationary properties of the biofilm.
  • anodic concepts i. for microbial electro-oxidation.
  • FIG. 2 shows an electrobioreactor 10 according to the invention for carrying out a sterile microbial electrosynthesis.
  • the electrobioreactor 10 has a cylindrical bioreactor vessel 12 open to one side 11 (usually upwards, ie against gravity), a lid 13 for closing the opening 14 of the bioreactor vessel 12 and an insert 15 reversibly insertable into the bioreactor vessel 12 ,
  • the insert 15 is cylindrically shaped like the bioreactor vessel 12, but has a smaller diameter.
  • the insert 15 is likewise designed to be open on the upper side 16 and can be attached via a flange 17 into a passage opening 18 with formations corresponding to the flange 17, not shown here, of the lid 13 of the electrobioreactor 10 and thus introduced into the bioreactor vessel 12.
  • the insert 15 is closed by a cover 19, which has two passage openings 20, wherein an electrode 21 is arranged in at least one passage opening 20. In the other passage opening 20, a second electrode can be arranged depending on the required electrode area.
  • the lid 19 has four openings 22 for the passage of fasteners, such as screws or the like, with which a fixation on the lid 13 of the electro bioreactor 10 takes place. The fastening means are not shown here. Between the flange 17 of the insert 15 and the lid 19 of the insert 15 not shown seals can be arranged.
  • the insert 15 has in the wall 23 three superimposed arranged through openings 24, which are each closed by a membrane 25.
  • the lid 13 for closing the opening 14 of the bioreactor vessel 12 is formed such that a flange 26 provided on the bioreactor vessel 12 formed at the opening 14 is gripped and sealed so that the lid 13 is fixed in its fitted state , Again, not shown seals and fasteners may be provided.
  • two reaction chambers 27, 28 are formed in the bioreactor vessel 12.
  • a first reaction chamber 27 is formed through the interior of the insert 15 and the second reaction chamber 28 through the space between the insert 15 and the inner wall of the bioreactor vessel 12.
  • Both reaction chambers 27, 28 are interconnected by the membrane (s) 25.
  • a magnetic stirrer not shown here, can also be located in the insert 15.
  • a through hole 30 is provided, through which an electrode 31 is guided.
  • a reference electrode 33 is arranged in the assembled state of the electrobioreactor 10 in the second reaction chamber 28.
  • a plurality of additional through holes 34 are provided in the lid 13, in the electrodes, probes, gassing or degassing, sampling or the like, not shown can be introduced. All through holes 20, 30, 32, 34 are, if they are not used with a suitable means such as a plug or the like. closed, otherwise no sterile operation is possible. LIST OF REFERENCE NUMBERS

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un électro-bioréacteur (10) pour réaliser une synthèse microbienne stérile et/ou ses pièces. L'invention propose un insert (15) avec une ouverture traversante (24) prévue dans une membrane (25) et un électro-bioréacteur (10) qui présente une cuve de bioréacteur (12) ouverte d'un côté et l'insert (15) qui peut être inséré de manière réversible dans la cuve de bioréacteur (12). Une première chambre de réaction (27) est prévue dans l'insert (15) et une seconde chambre de réaction (28) est prévue entre l'insert (15) et la paroi interne de la cuve de bioréacteur (12), la cuve de bioréacteur (12) et l'insert (15) étant adaptés à la stérilisation. L'invention concerne en outre un kit comprenant un électro-bioréacteur et/ou ses pièces ainsi que les utilisations de l'insert et de l'électro-bioréacteur (10).
PCT/EP2018/051228 2018-01-18 2018-01-18 Électro-bioréacteur et ses pièces pour réaliser des synthèses microbiennes stériles à usage unique ou réutilisable WO2019141365A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP18701701.7A EP3740557A1 (fr) 2018-01-18 2018-01-18 Électro-bioréacteur et ses pièces pour réaliser des synthèses microbiennes stériles à usage unique ou réutilisable
PCT/EP2018/051228 WO2019141365A1 (fr) 2018-01-18 2018-01-18 Électro-bioréacteur et ses pièces pour réaliser des synthèses microbiennes stériles à usage unique ou réutilisable

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2018/051228 WO2019141365A1 (fr) 2018-01-18 2018-01-18 Électro-bioréacteur et ses pièces pour réaliser des synthèses microbiennes stériles à usage unique ou réutilisable

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Publication Number Publication Date
WO2019141365A1 true WO2019141365A1 (fr) 2019-07-25

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69105317T2 (de) * 1990-03-12 1995-04-06 Difco Lab Inc System und Verfahren zum Verpacken von Petrischalen.
WO2000003447A1 (fr) 1998-07-09 2000-01-20 Michigan State University Procedes electrochimiques de generation d'une force motrice protonique de nature biologique et de regeneration d'un cofacteur nucleotidique de pyridine
WO2010117864A1 (fr) 2009-04-07 2010-10-14 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For An On Behalf Of Arizona State University Cellule électrolytique microbienne
WO2011062485A2 (fr) 2009-11-17 2011-05-26 Stichting Wetsus Centre Of Excellence For Sustainable Water Technology Dispositif bio-électrochimique et procédé pour améliorer un fluide
DE102011054364A1 (de) * 2011-10-10 2013-04-11 DASGIP Information and Process Technology GmbH Biotechnologische Vorrichtung mit einem Bioreaktor, Abgastemperiereinrichtung für einen Bioreaktor und Verfahren zum Behandeln eines Abgasstroms in einer biotechnologischen Vorrichtung
US20130256149A1 (en) 2012-03-28 2013-10-03 Arizona Science And Technology Enterprises Llc Microbial electrolysis cells and methods for the production of chemical products
DE102013224673A1 (de) 2013-12-02 2015-06-03 Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz Aufrüstset für Bioreaktoren zur Durchführung der mikrobiellen Bioelektrosynthese

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69105317T2 (de) * 1990-03-12 1995-04-06 Difco Lab Inc System und Verfahren zum Verpacken von Petrischalen.
WO2000003447A1 (fr) 1998-07-09 2000-01-20 Michigan State University Procedes electrochimiques de generation d'une force motrice protonique de nature biologique et de regeneration d'un cofacteur nucleotidique de pyridine
WO2010117864A1 (fr) 2009-04-07 2010-10-14 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For An On Behalf Of Arizona State University Cellule électrolytique microbienne
WO2011062485A2 (fr) 2009-11-17 2011-05-26 Stichting Wetsus Centre Of Excellence For Sustainable Water Technology Dispositif bio-électrochimique et procédé pour améliorer un fluide
DE102011054364A1 (de) * 2011-10-10 2013-04-11 DASGIP Information and Process Technology GmbH Biotechnologische Vorrichtung mit einem Bioreaktor, Abgastemperiereinrichtung für einen Bioreaktor und Verfahren zum Behandeln eines Abgasstroms in einer biotechnologischen Vorrichtung
US20130256149A1 (en) 2012-03-28 2013-10-03 Arizona Science And Technology Enterprises Llc Microbial electrolysis cells and methods for the production of chemical products
DE102013224673A1 (de) 2013-12-02 2015-06-03 Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz Aufrüstset für Bioreaktoren zur Durchführung der mikrobiellen Bioelektrosynthese

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