WO2019132510A3 - Recombinant yeast having mutated organelle and isoprenoid production method using same - Google Patents

Recombinant yeast having mutated organelle and isoprenoid production method using same Download PDF

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Publication number
WO2019132510A3
WO2019132510A3 PCT/KR2018/016663 KR2018016663W WO2019132510A3 WO 2019132510 A3 WO2019132510 A3 WO 2019132510A3 KR 2018016663 W KR2018016663 W KR 2018016663W WO 2019132510 A3 WO2019132510 A3 WO 2019132510A3
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WIPO (PCT)
Prior art keywords
organelle
mutated
isoprenoid
same
recombinant yeast
Prior art date
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PCT/KR2018/016663
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French (fr)
Korean (ko)
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WO2019132510A2 (en
Inventor
이평천
강현준
최보현
이건중
Original Assignee
아주대학교산학협력단
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Publication of WO2019132510A2 publication Critical patent/WO2019132510A2/en
Publication of WO2019132510A3 publication Critical patent/WO2019132510A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
    • C12Y103/99031—Phytoene desaturase (lycopene-forming) (1.3.99.31)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01001—Dimethylallyltranstransferase (2.5.1.1)
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    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01032—15-Cis-phytoene synthase (2.5.1.32)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y505/00—Intramolecular lyases (5.5)
    • C12Y505/01—Intramolecular lyases (5.5.1)
    • C12Y505/01019—Lycopene beta-cyclase (5.5.1.19)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a recombinant yeast in which an organelle is mutated by gene manipulation and a method for production of various isopreonoids by using the same. When a metabolic pathway of the useful product isoprenoid is introduced thereinto, a mutated yeast that is increased in the number or size of the organelle peroxisome can remarkably increase an isoprenoid production yield, compared to yeasts in which the organelle is not mutated, allowing the mass production of isoprenoid and fining useful applications in various industrial fields.
PCT/KR2018/016663 2017-12-27 2018-12-26 Recombinant yeast having mutated organelle and isoprenoid production method using same WO2019132510A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0181134 2017-12-27
KR20170181134 2017-12-27

Publications (2)

Publication Number Publication Date
WO2019132510A2 WO2019132510A2 (en) 2019-07-04
WO2019132510A3 true WO2019132510A3 (en) 2019-08-15

Family

ID=67067822

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/016663 WO2019132510A2 (en) 2017-12-27 2018-12-26 Recombinant yeast having mutated organelle and isoprenoid production method using same

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KR (2) KR102170446B1 (en)
WO (1) WO2019132510A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021204338A1 (en) 2020-04-08 2021-10-14 Københavns Universitet Production of geranyl diphosphate-derived compounds
CN113624855B (en) * 2020-12-29 2023-05-19 晨光生物科技集团股份有限公司 Method for detecting residual quantity of n-hexane and acetone solvents in crystal lycopene
CN116286880B (en) * 2023-02-09 2024-04-30 昆明理工大学 Peroxisome increment factor gene RkPEX and application thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017214133A2 (en) * 2016-06-06 2017-12-14 Provivi, Inc. Semi-biosynthetic production of fatty alcohols and fatty aldehydes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
FLYNN C. M.: "Identification and characterization of fungal sesquiterpenoid biosynthetic pathways", DISSERTATION, May 2016 (2016-05-01) - 31 December 2016 (2016-12-31), Minnesota, pages 10, 38, 42 - 103, XP055630489 *
KNOBLACH B.: "Phosphorylation-dependent activation of peroxisome proli- ferator protein PEX11 controls peroxisome abundance", J. BIOL. CHEM, vol. 285, no. 9, 26 February 2010 (2010-02-26), pages 6670 - 6680, XP055630467 *
MOTLEY A. M. ET AL.: "Pex3-anchored Atg36 tags peroxisomes for degradation in Saccharomyces cerevisiae", EMBO. JOURNAL, vol. 31, no. 13, 29 June 2012 (2012-06-29), pages 2852 - 2868, XP055630460 *
NIELSEN, J.: "Synthetic Biology for Engineering Acetyl Coenzyme A Meta- bolism in Yeast", MBIO, vol. 5, no. 6, 2014, pages e02153 - 14, XP055367939 *
TOWER R. J.: "The peroxin Pex34p functions with the Pex11 family of per- oxisomal divisional proteins to regulate the peroxisome population in yeast", MOL. BIOL. CELL, vol. 22, no. 10, 15 May 2011 (2011-05-15), pages 1727 - 1738, XP055630464 *
VERWAAL R.: "High-level production of beta-carotene in Saccharomyces cerevisiae by successive transformation with carotenogenic genes from Xanthophyllomyces dendrorhous", APPL ENVIRON MICOBIOL., vol. 73, no. 13, 2007, pages 4342 - 4350, XP002587086, doi:10.1128/AEM.02759-06 *

Also Published As

Publication number Publication date
KR20190079575A (en) 2019-07-05
KR20200039645A (en) 2020-04-16
WO2019132510A2 (en) 2019-07-04
KR102170446B1 (en) 2020-10-28
KR102170444B1 (en) 2020-10-28

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