WO2019126926A1

WO2019126926A1 - Frozen section embedding box

Info

Publication number: WO2019126926A1
Application number: PCT/CN2017/118255
Authority: WO
Inventors: 韦命余; 李翔; 李伟丽; 钱政江; 杨海洋; 彭敏琪; 陈瑜; 李霞
Original assignee: 中国科学院深圳先进技术研究院
Priority date: 2017-12-25
Filing date: 2017-12-25
Publication date: 2019-07-04

Abstract

A frozen section embedding box, comprising a barrel (1), and a bottom cap (2). The two ends of the barrel (1) are open, and the size of the barrel (1) is fitted to the size of a tissue block to be embedded; the bottom cap (2) comprises a base plate (21) and a side wall (22); the base plate (21) has a flat bottom; the side wall (22) has an annular shape and is protrudingly disposed on the base plate (21); the cross-sectional dimension of the side wall (22) is fitted to that of the barrel (1); the side wall (22) connects with the bottom end of the barrel (1). The embedding box has a simple structure and is conveniently operated. A tissue block is easy to be taken out when frozen, thus greatly improving test efficiency. Meanwhile, the embedding box has good quality and can be recycled for repeated uses, thus effectively reducing resource waste and environmental pollution.

Description

Frozen slice cassette

Technical field

The present disclosure relates to the field of tissue embedding technology, for example, to a frozen slice embedding cassette.

Background technique

Frozen Section is a method of rapidly cooling a tissue to a certain hardness under low temperature conditions and then slicing it. The method mainly includes taking, fixing, embedding, slicing and dyeing, and embedding is an indispensable step. Embedding is a process in which a tissue block is placed in an embedding box, and a certain amount of embedding agent is injected, and then the embedding cassette is placed in a freezing device for freezing. There are two kinds of embedding boxes commonly used in the prior art, one is a cylindrical cylindrical embedding box which is obtained by winding and setting a tin foil paper on a cylindrical object, and the other is a disposable one-time embedding box purchased. .

The manufacturing process of the above-mentioned first type of embedding box is troublesome, and it is necessary to find a solid cylinder of a suitable size and a flat bottom to ensure that the size of the embedding box produced is good and the bottom is flat; if the bottom is not flat, it is easy to cause the embedding box to fall over. The second type of embedding cassette is inconvenient to remove the tissue block after freezing, which increases the difficulty of the experimenter. At the same time, whether it is the embedding box made of foil paper or the one-time embedding box already on the market, there are problems of poor quality of the box, waste of resources and environmental pollution, which does not meet the current environmental protection requirements.

Summary of the invention

The present disclosure provides a frozen slice embedding cassette, which has a simple structure and convenient operation, and is easy to take out a tissue block after freezing.

A frozen slice embedding box comprising:

a cylinder, the two ends of the cylinder are open, and the size of the cylinder is adapted to the size of the tissue block to be embedded;

a bottom cover, the bottom cover includes a bottom plate and a side wall, the bottom of the bottom plate is flat, the side wall is annularly protruded on the bottom plate, and a cross-sectional dimension of the side wall and a horizontal direction of the cylindrical body The cross-sectional dimensions are adapted such that the side walls are joined to the bottom end of the barrel.

Optionally, the cylindrical body has a circular, elliptical or polygonal cross-sectional shape.

Optionally, the bottom end of the barrel is provided with a first card protrusion, and the side wall is provided with a second card protrusion, and the first card protrusion cooperates with the second card protrusion.

Optionally, a connecting belt is further included, the first end of the connecting belt is connected to the bottom end of the cylinder, and the second end of the connecting belt is connected to the bottom plate of the bottom cover.

Optionally, the thickness of the intermediate portion of the connecting strip is less than the thickness of the first end and the second end.

Optionally, the barrel, the bottom cover and the connecting belt are both made of plastic material.

Optionally, at least one brim is disposed on the bottom plate of the bottom cover, and the brim is in the same plane as the bottom plate.

Optionally, further comprising an ejection mechanism comprising a pressure plate and a push rod fixedly coupled to one end of the pressure plate, the outer size of the pressure plate being adapted to the inner dimensions of the barrel, The length of the push rod is greater than the length of the barrel.

Optionally, an upper cover is further included, and the upper cover is movably disposed at an upper end of the cylinder.

Optionally, the cylinder is further provided with a scale and a label slot. The embedded box cylinder of the present disclosure is closely connected with the bottom cover to ensure the reliability of use of the embedding box; the bottom cover is flat, which is favorable for the embedding box to be stably standing on the table top, preventing the embedding box from falling over; The open structure at both ends facilitates the pushing out of the frozen tissue in the cylinder from the end of the cylinder after opening the bottom cover; the embedded box has a simple structure and convenient operation, and the tissue block is easily taken out after freezing, thereby greatly improving the experimental efficiency.

The embedding box of the present disclosure is made of plastic material, the box body has good quality, can be sterilized under high temperature and high pressure conditions, can be recycled repeatedly, avoids the trouble of making the embedding box mold in each experiment, and effectively reduces Resource waste and environmental pollution.

DRAWINGS

1 is a schematic structural view of a frozen slice embedding case provided in Embodiment 1;

2 is a schematic structural view of a frozen slice embedding case provided in Embodiment 2;

3 is a schematic structural view of a frozen slice embedding case provided in the third embodiment.

In the picture:

1-barrel; 11-first card convex;

2- bottom cover; 21- bottom plate; 22-side wall; 23-hat 檐; 221-second card convex;

3-connecting belt;

4-launch mechanism; 41-pressure plate; 42-push rod;

5-Upper cover.

Detailed ways

Embodiment 1

As shown in Fig. 1, this embodiment provides a frozen slice embedding cassette for embedding tissue blocks in histological diagnosis or research. The embedding box mainly comprises a cylinder body 1 and a bottom cover 2, wherein the cylinder body 1 is a cylindrical structure with open ends, and the bottom end of the cylindrical structure is detachably connected with the bottom cover 2. Optionally, the cross-sectional shape of the cylinder 1 can be set to a circular shape, an elliptical shape, a regular polygonal shape or the like according to the actual shape and experimental requirements of the tissue block. In this embodiment, a circular cross-shaped structure with simple structure and good versatility can be adopted. The cross section, that is, the entire cylinder 1 has a cylindrical cylindrical structure. Optionally, the inner diameter and the height of the barrel 1 are respectively adapted to the lateral and longitudinal dimensions of the tissue block to be embedded. Alternatively, the height of the barrel 1 in the embodiment is 2.0-2.5 cm, and the tube The body 1 is further provided with at least one of a scale and a label slot. The setting of the scale facilitates the experimenter to determine the amount of the embedded tissue block added to the barrel 1. The label slot is used to paste information related to the tissue block, which is convenient for the experimenter. Check it out. The bottom cover 2 includes a bottom plate 21 and a side wall 22, and the bottom of the bottom plate 21 is flat, so as to stably place the embedding box on the table top, preventing the embedding box from falling over during the experiment; the side wall 22 is convexly disposed on the bottom plate 21. The annular structure may be a ring shape, and the outer diameter of the ring is adapted to the inner diameter of the barrel 1 to achieve an interference fit with the barrel 1 so as to tightly fit the side wall 22 The bottom end of the inner cylinder 1. Optionally, the bottom plate 21 is further provided with a cap 23 for conveniently opening the cover, and the cap 23 is in the same plane as the bottom plate 21, so as to avoid affecting the flatness of the bottom plate 21; here, the number of the caps 23 may be one, A plurality of them may be uniformly disposed in the circumferential direction of the bottom plate 21 as needed.

Optionally, the bottom end of the inner cylinder 1 and the side wall 22 are respectively provided with fastening structures for tightly and firmly connecting the two to ensure the connection reliability and sealing effect of the embedding box during use. Alternatively, the fastening structure may be an internal thread provided at the bottom end of the barrel 1 and an external thread provided on the side wall 22, and the side wall 22 is screwedly connected to the barrel 1. In this embodiment, the fastening structure is a first protrusion 11 disposed inside the bottom end of the barrel 1 and a second protrusion 221 disposed outside the side wall 22, and the side wall 22 is inserted into the barrel. In the first embodiment, the first card protrusion 11 and the second card protrusion 221 are cross-fitted to each other to achieve a fastening connection between the barrel 1 and the bottom cover 2. Here, the distance between the first engaging projection 11 and the bottom end of the cylindrical body 1 should be adapted to the distance between the second engaging projection 221 and the bottom plate 21 to ensure that the bottom end of the cylindrical body 1 can abut against the end surface of the bottom plate 21.

Optionally, the embedding box further comprises a connecting strip 3, the first end of the connecting strip 3 is connected with the bottom end of the barrel 1, the second end of the connecting strip 3 is connected with the bottom plate 21, and the connecting strip 3 is arranged to make the bottom cover 2 is integrated with the cylinder 1 to effectively prevent the bottom cover 2 from being lost or selected incorrectly, thereby greatly improving the test efficiency. Optionally, the thickness of the first end of the connecting strip 3 connected to the tubular body 1 is the same as the thickness of the side arm of the tubular body 1, and the second end of the connecting strip 3 connected to the bottom cover 2 is consistent with the thickness of the bottom plate 21, and the setting can Increase the strength of the two connecting ends of the connecting strip to prevent breakage during use; and the thickness of the middle section of the connecting strip 3 is smaller than the thickness of the two ends. Since the intermediate section is relatively small in force, appropriately reducing the thickness thereof is beneficial to The bending of the connecting belt 3 is convenient to use.

Optionally, the above-mentioned cylinder 1, the bottom cover 2 and the connecting belt 3 are made of a plastic material, and may be a transparent PP (polypropylene) material. The PP material does not contain any heavy metal ions, does not pollute the tissue block and the environment, and the embedding box made of PP material is of good quality and can be in the condition of 121 ° C / 15 psi (psi: psi) Autoclaving is carried out, so that recycling can be repeated, avoiding the trouble of making a cassette mold in each experiment. At the same time, the inner wall of the cylinder 1 made of plastic material is smooth, and because the two ends of the cylinder 1 have an open structure, it is convenient to smoothly push out the frozen tissue block in the cylinder from one end of the cylinder 1 after opening the bottom cover 2. In addition, the outer wall of the cylinder 1 or the bottom of the bottom cover 2 can be written by sanding to facilitate the marking of a plurality of different embedding boxes. Optionally, the above-mentioned cylinder 1, the bottom cover 2 and the connecting belt 3 are arranged in a one-piece structure, and can be integrally injection molded by an injection mold or directly printed by a 3D printing device. The integrated structure makes the embedding box easier to manufacture, more convenient to use, saves manufacturing cost, and is beneficial to manufacturers for mass production, and has good economic benefits.

In summary, the embedding box has a simple structure, convenient operation, and easy to take out the tissue block after freezing, thereby greatly improving the experiment efficiency; and at the same time, the embedding box can be repeatedly used repeatedly, thereby effectively reducing resource waste and environmental pollution.

Embodiment 2

As shown in FIG. 2, a frozen slice embedding box provided in the second embodiment is provided with an ejection mechanism 4 in addition to all the structures of the first embodiment.

Optionally, the pushing mechanism 4 includes a pressure plate 41 and a push rod 42 fixedly connected to one end of the pressure plate 41, wherein the outer size of the pressure plate 41 is adapted to the inner size of the cylinder 1 (ie, the clearance fit), so that The platen 41 slides smoothly in the cylinder 1 to smoothly push out the frozen tissue block. Similarly, in the present embodiment, the pressure plate 41 may have a circular shape for use in conjunction with the cylindrical tubular body 1 of the first embodiment. In this embodiment, the length of the push rod 42 should be greater than the length of the barrel 1. This arrangement is convenient for the experimenter to perform the hand-held operation on the one hand, and can ensure that the tissue block is completely pushed out of the barrel 1 on the other hand. In this embodiment, by setting the pushing mechanism, the pushing out of the finger or other objects is more stable, convenient, and sanitary than the first embodiment, thereby effectively improving the quality of the frozen section and the efficiency of the experiment.

Embodiment 3

As shown in FIG. 3, a frozen slice embedding box provided in the third embodiment is provided with an upper cover 5 in addition to all the structures of the first embodiment. The radial dimension of the upper cover 5 is slightly larger than the radial dimension of the cylinder 1 in order to cover the upper cover 5 to the upper end of the cylinder 1. By providing the upper cover 5, it is possible to prevent dust, bacteria, etc. from entering the cylinder 1 from contaminating the tissue block after the embedding cassette is loaded into the tissue block, thereby reducing the influence of the external environment on the frozen section experiment.

Of course, in this embodiment, the pushing mechanism 4 as shown in the second embodiment can also be disposed at the same time. By adding the pushing mechanism 4 and the upper cover 5 to the embedding box, the embedding box can be ensured to have the best performance and is effective. Improve its practical value.

Industrial applicability

The frozen slice embedding box of the present disclosure has a simple structure and convenient operation, and is easy to take out the tissue block after freezing, thereby greatly improving the experiment efficiency.

Claims

A frozen slice embedding box comprising:

a cylinder (1), the two ends of the cylinder (1) are open, and the size of the cylinder (1) is adapted to the size of the tissue block to be embedded;

a bottom cover (2), the bottom cover (2) includes a bottom plate (21) and a side wall (22), the bottom of the bottom plate (21) is flat, and the side wall (22) is annularly protruded from the On the bottom plate (21), the cross-sectional dimension of the side wall (22) is adapted to the cross-sectional dimension of the cylinder (1), the side wall (22) and the bottom of the cylinder (1) End connection.
A frozen slice embedding case according to claim 1, wherein the cylindrical body (1) has a circular, elliptical or polygonal cross-sectional shape.
A frozen slice embedding case according to claim 2, wherein the bottom end of the barrel (1) is provided with a first card protrusion (11), and the side wall (22) is provided with a second card The protrusion (221), the first card protrusion (11) is matched with the second card protrusion (221).
A frozen slice embedding cassette according to claim 3, further comprising a connecting strip (3), the first end of the connecting strip (3) being connected to the bottom end of the barrel (1), the connection The second end of the belt (3) is coupled to the bottom plate (21).
A frozen slice embedding cassette according to claim 4, wherein the thickness of the intermediate portion of the connecting strip (3) is smaller than the thickness of the first end and the second end.
A frozen slice embedding case according to claim 5, wherein the cylindrical body (1), the bottom cover (2) and the connecting tape (3) are each made of a plastic material.
A frozen slice embedding cassette according to any one of claims 1 to 6, wherein the bottom plate (21) is provided with at least one brim (23), the brim (23) and the bottom plate (21) ) in the same plane.
A frozen slice embedding cassette according to claim 7, further comprising an ejection mechanism (4), the ejection mechanism (4) comprising a pressure plate (41) and a push rod fixedly coupled to one end of the pressure plate (41) ( 42) The outer dimensions of the pressure plate (41) are adapted to the inner dimensions of the cylinder (1), the length of the push rod (42) being greater than the length of the cylinder (1).
A frozen slice embedding cassette according to claim 7, further comprising an upper cover (5), the upper cover (5) being movably covered at an upper end of the cylindrical body (1).
A frozen slice embedding case according to claim 7, wherein at least one of a scale and a label groove is further provided on the barrel (1).