WO2019116209A1 - Puce microfluidique et système d'image microscopique - Google Patents

Puce microfluidique et système d'image microscopique Download PDF

Info

Publication number
WO2019116209A1
WO2019116209A1 PCT/IB2018/059851 IB2018059851W WO2019116209A1 WO 2019116209 A1 WO2019116209 A1 WO 2019116209A1 IB 2018059851 W IB2018059851 W IB 2018059851W WO 2019116209 A1 WO2019116209 A1 WO 2019116209A1
Authority
WO
WIPO (PCT)
Prior art keywords
groove portion
feeding
sample reservoir
microfluidic chip
rotation center
Prior art date
Application number
PCT/IB2018/059851
Other languages
English (en)
Inventor
Hsien-Chang Chang
Wen-Chien Ko
Po-Lin Chen
Chung-Hsin SHIH
Original Assignee
National Cheng Kung University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from TW107139015A external-priority patent/TWI685651B/zh
Application filed by National Cheng Kung University filed Critical National Cheng Kung University
Priority to US16/769,332 priority Critical patent/US20200306755A1/en
Publication of WO2019116209A1 publication Critical patent/WO2019116209A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/0008—Microscopes having a simple construction, e.g. portable microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/0088—Inverse microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Definitions

  • the machine case assembly includes a machine case, and a light source unit that is mounted to the machine case and that is operable to emit light downwardly.
  • the image capture device is mounted to the machine case, and includes a focus adjusting module and a microscopic image module that is mounted to the focus adjusting module.
  • the microscopic image module is drivable by the focus adjusting module to move vertically.
  • the microscopic image module includes an objective lens that is within the lighting area of the light source unit and that is adapted to capture image, a lens barrel that extends vertically and that is connected to a lower end of the objective lens and a photodetector that is connected to a lower end of the lens barrel and that is adapted for capturing images through the objective lens.
  • FIG. 3 is a top view of the microfluidic chip
  • FIG. 4 is a sectional view of the microfluidic chip, taken along line A-A of FIG. 3;
  • FIG. 13 is an exploded perspective view of a variation of the microfluidic chip.
  • the microfluidic chip Referring to FIGS. 2, 3 and 4, the microfluidic chip
  • the chip main body 4 may be made of a transparent or opaque hydrophobic material, such as poly (methyl methacrylate) (PMMA), cyclic olefin copolymer (COP), polycarbonate (PC), polyamide (PA), polypropylene (PP), etc.
  • PMMA poly (methyl methacrylate)
  • COP cyclic olefin copolymer
  • PC polycarbonate
  • PA polyamide
  • PP polypropylene
  • the chip main body 4 has a vertical rotation center 40 and an identification code 30 disposed on a top surface thereof.
  • the chip main body 4 further has a sample reservoir 41, a liquid groove 42, a plurality of reaction chambers 43, a first inlet channel 44 and a plurality of second inlet channels 45.
  • the feeding groove portion 421 extends around the rotation center 40 and the sample reservoir 41, and has a first feeding end 422 and a second feeding end 423 opposite to the first feeding end 422.
  • the feeding groove portion 421 extends gradually away from the rotation center 40 from the first feeding end 422 to the second feeding end 423.
  • the feeding groove portion 421 extends counterclockwisely (as seen from the top view of FIG. 3) along a path shaped as an involute of a circle such that the distance between the second feeding end 423 and the rotation center 40 is greater than the distance between the first feeding end 422 and the rotation center 40.
  • the reagents 6 are respectively fixed to sides of the reaction chambers 43, such as bottom sides or side walls.
  • the reagents 6 are formed by coating and drying reacting reagents on the sides, and dissolve in and react with the sample solution.
  • the reagents 6 may be antibiotics, antibodies for immunosorbent reaction, probes for detecting genetic materials (e.g., DNA) or other substances that are capable of reacting with the sample solution.
  • the reacting solution is prevented from entering adjacent grooves without action of the centrifugal force and is prevented from backflowing from the reaction chambers 43, which may contaminate the reacting solution or affect the test results.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Optics & Photonics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne une puce microfluidique qui comprend un corps principal de puce, qui a un centre de rotation, un réservoir d'échantillon, une rainure de liquide, de multiples chambres de réaction, un premier canal d'entrée et de multiples seconds canaux d'entrée, et une membrane de scellage reliée au corps principal de puce. La rainure de liquide a une partie à rainure d'alimentation qui s'étend autour du centre de rotation et du réservoir d'échantillon, et de multiples parties à rainure de dosage qui s'étendent à l'opposé du centre de rotation. Le premier canal d'entrée fait communiquer le réservoir d'échantillon et la partie à rainure d'alimentation. Chaque second canal d'entrée fait communiquer une partie à rainure de dosage respective et une chambre de réaction respective. La profondeur du premier canal d'entrée est inférieure à celle du réservoir d'échantillon et de la partie rainure d'alimentation. La profondeur de chaque second canal d'entrée est inférieure à celles de la partie à rainure de dosage respective, de la chambre de réaction respective et du premier canal d'entrée.
PCT/IB2018/059851 2017-12-12 2018-12-11 Puce microfluidique et système d'image microscopique WO2019116209A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/769,332 US20200306755A1 (en) 2017-12-12 2018-12-11 Microfluidic chip and microscopic image system

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201762597899P 2017-12-12 2017-12-12
US62/597,899 2017-12-12
TW107139015A TWI685651B (zh) 2017-12-12 2018-11-02 微流體晶片
TW107139015 2018-11-02

Publications (1)

Publication Number Publication Date
WO2019116209A1 true WO2019116209A1 (fr) 2019-06-20

Family

ID=66819034

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2018/059851 WO2019116209A1 (fr) 2017-12-12 2018-12-11 Puce microfluidique et système d'image microscopique

Country Status (1)

Country Link
WO (1) WO2019116209A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113115587A (zh) * 2019-11-13 2021-07-13 京东方科技集团股份有限公司 检测芯片
WO2021189064A1 (fr) * 2020-03-16 2021-09-23 Siemens Healthcare Diagnostics Inc. Porte-échantillon, ensembles de station de pcr et procédés de fonctionnement de systèmes de test par pcr
WO2023126617A1 (fr) * 2021-12-31 2023-07-06 Biomerieux Carte d'analyse d'un echantillon biologique, procede de fabrication et de controle qualite
CN117233412A (zh) * 2023-11-13 2023-12-15 成都斯马特科技有限公司 微流控生化试剂盘及生化检验分析方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202049111U (zh) * 2011-01-25 2011-11-23 山东师范大学 一种用于微流控分析的激光诱导荧光检测器
CN203249870U (zh) * 2013-05-13 2013-10-23 兰州大学 用于荧光检测器的成像辅助调节焦距和位置系统
CN205580999U (zh) * 2016-04-21 2016-09-14 深圳市博瑞生物科技有限公司 一种基于荧光显微镜的微流控芯片液滴检测系统
US20170122904A1 (en) * 2010-10-07 2017-05-04 Sandia Corporation Fluid delivery manifolds and microfluidic systems

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170122904A1 (en) * 2010-10-07 2017-05-04 Sandia Corporation Fluid delivery manifolds and microfluidic systems
CN202049111U (zh) * 2011-01-25 2011-11-23 山东师范大学 一种用于微流控分析的激光诱导荧光检测器
CN203249870U (zh) * 2013-05-13 2013-10-23 兰州大学 用于荧光检测器的成像辅助调节焦距和位置系统
CN205580999U (zh) * 2016-04-21 2016-09-14 深圳市博瑞生物科技有限公司 一种基于荧光显微镜的微流控芯片液滴检测系统

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113115587A (zh) * 2019-11-13 2021-07-13 京东方科技集团股份有限公司 检测芯片
CN113115587B (zh) * 2019-11-13 2023-06-20 京东方科技集团股份有限公司 检测芯片
WO2021189064A1 (fr) * 2020-03-16 2021-09-23 Siemens Healthcare Diagnostics Inc. Porte-échantillon, ensembles de station de pcr et procédés de fonctionnement de systèmes de test par pcr
WO2023126617A1 (fr) * 2021-12-31 2023-07-06 Biomerieux Carte d'analyse d'un echantillon biologique, procede de fabrication et de controle qualite
FR3131632A1 (fr) * 2021-12-31 2023-07-07 Biomerieux Carte d’analyse d'un échantillon biologique, procédé de fabrication et de contrôle qualité
CN117233412A (zh) * 2023-11-13 2023-12-15 成都斯马特科技有限公司 微流控生化试剂盘及生化检验分析方法
CN117233412B (zh) * 2023-11-13 2024-02-02 成都斯马特科技有限公司 微流控生化试剂盘及生化检验分析方法

Similar Documents

Publication Publication Date Title
US20200306755A1 (en) Microfluidic chip and microscopic image system
WO2019116209A1 (fr) Puce microfluidique et système d'image microscopique
US7276720B2 (en) Apparatus and methods for analyzing samples
US9207249B2 (en) Automated system for handling microfluidic devices
US8932538B2 (en) Apparatus for analyzing sample
US20060012793A1 (en) Apparatus and methods for analyzing samples
US9308530B2 (en) Reaction container plate and reaction treatment apparatus
US11325120B2 (en) Specimen treatment chip, specimen treatment apparatus, and specimen treatment method
EP3227667B1 (fr) Appareil de test et son procédé de commande
CA2435789A1 (fr) Systeme et cartouche de traitement d'un prelevement biologique
WO2009112594A2 (fr) Essais
JP7429990B2 (ja) フローアッセイアナライザ
JP2017519996A (ja) マイクロ流体チップ、その製造方法及びそれを用いた分析装置
US20180104687A1 (en) Fluid loading into a microfluidic device
JPWO2008132873A1 (ja) 反応容器プレート及び反応処理方法
CN109475864B (zh) 用于生物试样的多级一次性筒
WO2009129397A2 (fr) Distributeur à haut rendement
US6645737B2 (en) Method for maintaining test accuracy within a microbiological test array
US20210285908A1 (en) Consumable system for molecule detection assays
JP4962227B2 (ja) 反応容器プレート及びその反応容器プレートを用いた反応処理方法
WO2021259497A1 (fr) Puce microbiologique et dispositif de pompage
JP2006284324A (ja) マイクロ総合分析システム

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18889059

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18889059

Country of ref document: EP

Kind code of ref document: A1