WO2019113373A3 - Products and methods for assessing and increasing klotho protein levels - Google Patents

Products and methods for assessing and increasing klotho protein levels Download PDF

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Publication number
WO2019113373A3
WO2019113373A3 PCT/US2018/064333 US2018064333W WO2019113373A3 WO 2019113373 A3 WO2019113373 A3 WO 2019113373A3 US 2018064333 W US2018064333 W US 2018064333W WO 2019113373 A3 WO2019113373 A3 WO 2019113373A3
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WO
WIPO (PCT)
Prior art keywords
klotho protein
methods
protein levels
products
compositions
Prior art date
Application number
PCT/US2018/064333
Other languages
French (fr)
Other versions
WO2019113373A2 (en
Inventor
Joseph F. Tarsio
Dinesh RATURI
William Ian Ramage
James R. PLANTE
Original Assignee
Klotho Therapeutics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/208,552 external-priority patent/US20190169593A1/en
Application filed by Klotho Therapeutics, Inc. filed Critical Klotho Therapeutics, Inc.
Priority to US16/769,800 priority Critical patent/US20210371839A1/en
Priority to JP2020531132A priority patent/JP7290340B2/en
Priority to AU2018378802A priority patent/AU2018378802A1/en
Priority to KR1020207019460A priority patent/KR20200095538A/en
Priority to CA3101100A priority patent/CA3101100A1/en
Priority to EP18885984.7A priority patent/EP3720474A4/en
Publication of WO2019113373A2 publication Critical patent/WO2019113373A2/en
Publication of WO2019113373A3 publication Critical patent/WO2019113373A3/en
Priority to JP2023086449A priority patent/JP2023113737A/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01031—6-Phosphogluconolactonase (3.1.1.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01031—Beta-glucuronidase (3.2.1.31)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Disclosed are products and methods for monitoring Klotho protein levels and for stabilizing Klotho protein in a mammalian blood sample, especially at room temperature or without freezing, for a period of time. Methods of detecting and quantifying Klotho protein levels, particularly endogenous and/or exogenous soluble alpha Klotho protein levels, methods of diagnosing Klotho protein deficiency, and methods of increasing Klotho protein levels or production, particularly endogenous and/or exogenous soluble alpha Klotho protein level(s), expression, or production, in a mammalian subject, and products useful in performing the same, including diagnostic kits and compositions for treating Klotho protein deficiency, are disclosed. Compositions are configured or formulated to augment natural soluble alpha Klotho protein production, attenuate Klotho protein damage or degradation, and/or supplement Klotho protein levels with exogenous, recombinant protein. Treatment methods and uses include administration of the compositions to human or non-human mammalian subjects.
PCT/US2018/064333 2017-12-06 2018-12-06 Products and methods for assessing and increasing klotho protein levels WO2019113373A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US16/769,800 US20210371839A1 (en) 2017-12-06 2018-12-06 Products and Methods for Assessing and Increasing Klotho Protein Levels
JP2020531132A JP7290340B2 (en) 2017-12-06 2018-12-06 Products and methods for assessing and improving Klotho protein levels
AU2018378802A AU2018378802A1 (en) 2017-12-06 2018-12-06 Products and methods for assessing and increasing Klotho protein levels
KR1020207019460A KR20200095538A (en) 2017-12-06 2018-12-06 Products and methods for assessing and increasing klotoprotein levels
CA3101100A CA3101100A1 (en) 2017-12-06 2018-12-06 Products and methods for assessing and increasing klotho protein levels
EP18885984.7A EP3720474A4 (en) 2017-12-06 2018-12-06 Products and methods for assessing and increasing klotho protein levels
JP2023086449A JP2023113737A (en) 2017-12-06 2023-05-25 Products and methods for assessing and increasing klotho protein levels

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201762595567P 2017-12-06 2017-12-06
US62/595,567 2017-12-06
US201862666868P 2018-05-04 2018-05-04
US62/666,868 2018-05-04
US201862692195P 2018-06-29 2018-06-29
US62/692,195 2018-06-29
US16/208,552 2018-12-03
US16/208,552 US20190169593A1 (en) 2016-06-02 2018-12-03 Products and methods for assessing and increasing klotho protein levels

Publications (2)

Publication Number Publication Date
WO2019113373A2 WO2019113373A2 (en) 2019-06-13
WO2019113373A3 true WO2019113373A3 (en) 2019-09-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2018/064333 WO2019113373A2 (en) 2017-12-06 2018-12-06 Products and methods for assessing and increasing klotho protein levels

Country Status (6)

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EP (1) EP3720474A4 (en)
JP (2) JP7290340B2 (en)
KR (1) KR20200095538A (en)
AU (1) AU2018378802A1 (en)
CA (1) CA3101100A1 (en)
WO (1) WO2019113373A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020160617A1 (en) * 2019-02-05 2020-08-13 Helium 3 Biotech Pty Ltd A membrane protein expression and distribution modulating composition and method of use thereof
CN111184728B (en) * 2020-02-20 2023-04-04 上海市儿童医院 Application of trehalose in nephrotic syndrome
CN112114075B (en) * 2020-09-17 2022-12-09 哈尔滨珍宝制药有限公司 Construction method and quality evaluation method of notopterygium root Shengshi Shang Zhiwen map
CN113350383B (en) * 2020-11-26 2023-03-24 内蒙古伊利实业集团股份有限公司 Bifidobacterium lactis BL-99 capable of resisting oxidation and regulating blood pressure and application thereof
CN114441696A (en) * 2021-12-22 2022-05-06 南京格亚医药科技有限公司 Method for detecting eribulin mesylate key intermediate

Citations (6)

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Publication number Priority date Publication date Assignee Title
US20060248398A1 (en) * 2005-05-02 2006-11-02 Neel Gary T Computer interface for diagnostic meter
US20080097244A1 (en) * 2005-01-19 2008-04-24 Theo Arnitz Test unit for carrying out a one-time testing of a body fluid
WO2011084452A1 (en) * 2009-12-16 2011-07-14 Eli Lilly And Company Therapeutic uses of soluble alpha-klotho
WO2012018638A2 (en) * 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US20120178699A1 (en) * 2007-05-08 2012-07-12 Tel Hashomer Medical Research Infrastructure And Services Ltd. Klotho protein and related compounds for the treatment and diagnosis of cancer
WO2018098375A1 (en) * 2016-11-22 2018-05-31 Klotho Therapeutics, Inc. Novel recombinant klotho proteins and compositions and methods involving the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200936156A (en) 2008-01-28 2009-09-01 Novartis Ag Methods and compositions using Klotho-FGF fusion polypeptides
CA2955039C (en) * 2014-07-31 2021-05-04 The Governing Council Of The University Of Toronto Antibodies with high affinity for aklotho

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080097244A1 (en) * 2005-01-19 2008-04-24 Theo Arnitz Test unit for carrying out a one-time testing of a body fluid
US20060248398A1 (en) * 2005-05-02 2006-11-02 Neel Gary T Computer interface for diagnostic meter
US20120178699A1 (en) * 2007-05-08 2012-07-12 Tel Hashomer Medical Research Infrastructure And Services Ltd. Klotho protein and related compounds for the treatment and diagnosis of cancer
WO2011084452A1 (en) * 2009-12-16 2011-07-14 Eli Lilly And Company Therapeutic uses of soluble alpha-klotho
WO2012018638A2 (en) * 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
WO2018098375A1 (en) * 2016-11-22 2018-05-31 Klotho Therapeutics, Inc. Novel recombinant klotho proteins and compositions and methods involving the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HU , MC ET AL.: "Secreted Klotho and Chronic Kidney Disease", ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY, vol. 728, 2012, pages 126 - 157, XP055574185, doi:10.1007/978-1-4614-0887-1_9 *
TOHYAMA, O ET AL.: "Klotho Is a Novel Beta-Glucuronidase Capable of Hydrolyzing Steroid Beta-Glucuronides", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, no. 11, 29 December 2003 (2003-12-29), pages 9777 - 9784, XP002523385, doi:10.1074/JBC.M312392200 *

Also Published As

Publication number Publication date
JP7290340B2 (en) 2023-06-13
EP3720474A4 (en) 2021-12-29
JP2021508037A (en) 2021-02-25
JP2023113737A (en) 2023-08-16
EP3720474A2 (en) 2020-10-14
CA3101100A1 (en) 2019-06-13
AU2018378802A1 (en) 2020-07-23
KR20200095538A (en) 2020-08-10
WO2019113373A2 (en) 2019-06-13

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