WO2019100630A1 - 用于太赫兹光谱测量的载样组件及太赫兹光谱测试方法 - Google Patents

用于太赫兹光谱测量的载样组件及太赫兹光谱测试方法 Download PDF

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WO2019100630A1
WO2019100630A1 PCT/CN2018/080594 CN2018080594W WO2019100630A1 WO 2019100630 A1 WO2019100630 A1 WO 2019100630A1 CN 2018080594 W CN2018080594 W CN 2018080594W WO 2019100630 A1 WO2019100630 A1 WO 2019100630A1
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sample
terahertz
layer
signal
adsorption layer
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PCT/CN2018/080594
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English (en)
French (fr)
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王志琪
丁庆
冯军正
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深圳市太赫兹科技创新研究院
深圳市太赫兹科技创新研究院有限公司
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Publication of WO2019100630A1 publication Critical patent/WO2019100630A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/3581—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
    • G01N21/3586—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation by Terahertz time domain spectroscopy [THz-TDS]

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  • the invention relates to the field of terahertz detection technology, in particular to a sample loading component for terahertz spectroscopy measurement and a terahertz spectroscopy test method.
  • the terahertz wave refers to an electromagnetic wave with a frequency in the range of 0.1-10 THz, which is between the microwave and the infrared. Since the terahertz wave is sensitive to the vibration and rotation mode of the organic molecule, the terahertz spectrum of the substance is fingerprinted, and the terahertz time The domain spectroscopy technology can obtain amplitude and phase information at the same time, and the information is rich, and the terahertz spectrum of the substance is the fingerprint spectrum of the substance, which has the characteristics of high permeability, low energy, transientness, sharpness, high signal-to-noise ratio, etc. The characteristics make terahertz non-destructive testing technology have important applications in many methods such as biomedicine, biochemistry, and chemical engineering.
  • the method for preparing samples by terahertz technology is mainly powder tableting method.
  • the method is simple, the pulverizing and sieving step takes a long time, and the sample amount required for tableting is large, and at least 10 mg of sample and polyethylene are required. Mixing the tablet (13 mm diameter round sheet) is not suitable for the micro-sample tableting method.
  • the terahertz technique for liquid samples is primarily a liquid pool method.
  • the commonly used methods for sample preparation in terahertz detection are limited, and there are many samples required, and the sample consumption is large, which is not suitable for the detection when the sample is small.
  • a sample loading assembly for terahertz spectroscopy comprising: a support layer, a first adsorption layer and a second adsorption layer;
  • the support layer is interposed between the first adsorption layer and the second adsorption layer, so that the first adsorption layer and the second adsorption layer form a gap of 0.2 mm to 0.5 mm;
  • the first adsorption layer and the second adsorption layer are both nitrocellulose membranes.
  • the support layer is provided with a sample hole such that the first adsorption layer is spaced apart from the second adsorption layer.
  • the first fixed layer and the second fixed layer are stacked on the surface of the first adsorption layer away from the support layer, and the second fixed layer is stacked on the surface The second adsorption layer is away from the surface of the support layer;
  • a first test window is defined on the first fixed layer, and a second test window is opened on the second fixed layer, and the first test window and the second test window are both in communication with the sample hole.
  • the first fixed layer and the second fixed layer have a thickness of 0.2 mm to 0.5 mm, respectively.
  • the nitrocellulose membrane has a pore diameter of from 0.1 ⁇ m to 0.65 ⁇ m.
  • the support layer is a copper sheet, an iron sheet, a silver sheet or a gold sheet.
  • a terahertz spectroscopy test method comprising the following steps:
  • the above-mentioned sample loading component is placed in a terahertz spectrometer, and under a purge of a protective gas, a no-load test is performed to obtain a reference signal;
  • the sampled component to which the sample solution is added is tested by a terahertz spectrometer under a purge of a protective gas to obtain a sample signal;
  • a terahertz absorption spectrum of the sample is obtained by the reference signal and the sample signal.
  • the terahertz spectrometer is a THz-4000 terahertz spectrometer of Teraview; the terahertz spectral width is 0.06 THz to 4.0 THz; during the scanning of the signal, the scanning range is 0-1200 ps.
  • the acquisition rate is 30 scans/s and the resolution of the terahertz spectrum is 1.2 cm -1 .
  • the step of obtaining a terahertz absorption spectrum of the sample by using the reference signal and the sample signal comprises:
  • ⁇ is the electromagnetic wave frequency
  • t is the time
  • i is the imaginary unit
  • Er(t) is the time domain signal of the reference signal
  • Es(t) is the time domain signal of the sample signal
  • Er( ⁇ ) is the frequency domain of the reference signal
  • the signal, Es( ⁇ ) is the frequency domain signal of the sample signal
  • Ar( ⁇ ) is the amplitude of the electric field of the reference signal
  • As( ⁇ ) is the amplitude of the electric field of the sample signal
  • ⁇ r( ⁇ ) is the phase of the electric field of the reference signal
  • ⁇ s( ⁇ ) is the phase of the electric field of the sample signal
  • the data processing model based on the Fresnel formula is used to extract the refractive index n( ⁇ ) and the absorption coefficient ⁇ ( ⁇ ) of the sample.
  • the formula of the data processing model based on the Fresnel formula is:
  • ⁇ ( ⁇ ) is the ratio of the amplitudes of the sample signal and the reference signal
  • ⁇ ( ⁇ ) is the phase difference between the sample signal and the reference signal
  • d is the thickness of the sample
  • c is the propagation velocity of the electromagnetic wave in vacuum.
  • the concentration of the sample solution is from 0.1 g/mL to 2 g/mL.
  • the above-mentioned sample assembly for terahertz spectroscopy and the terahertz spectroscopy test method use two layers of nitrocellulose membranes spaced apart from each other for adsorption test samples, since the absorption coefficient of the nitrocellulose membrane is similar to that of the polyethylene material. It can be applied to terahertz spectroscopy measurement, and the two layers of nitrocellulose membrane with a spacing of 0.2mm ⁇ 0.5mm are combined to form the adsorption layer. Only a small amount of sample solution is added to the adsorption layer, and the sample solution can be chromatographed to nitric acid.
  • the test can be carried out even if the concentration of the sample solution or the sample amount is small, the test can be accurately performed, and the use of the polyethylene powder tableting method is avoided. Or the sample preparation method of the polyvinylidene fluoride film impregnated sample solution, the required sample amount is small, the sample consumption is small, and it can be applied to the detection of less samples.
  • FIG. 1 is a cross-sectional view of a sample loading assembly for terahertz spectroscopy measurement of an embodiment
  • FIG. 2 is an exploded perspective view of the sample loading assembly for terahertz spectroscopy shown in FIG. 1;
  • FIG. 3 is a flow chart of a terahertz spectroscopy test method of an embodiment
  • sample assembly A is a terahertz absorption spectrum of sample assembly A, sample assembly B, sample assembly C, and sample assembly D;
  • sample assembly E is a terahertz absorption spectrum of sample assembly E, sample assembly F, sample assembly G, sample assembly H, and sample assembly I;
  • sample assembly J is a terahertz absorption spectrum of sample assembly J, sample assembly K, sample assembly L, sample assembly M, and sample assembly N.
  • sample loading component for terahertz spectroscopy measurement and the terahertz spectroscopy test method will be further described in detail below with reference to the specific embodiments and the accompanying drawings.
  • the sample loading assembly 100 for terahertz spectroscopy includes a first adsorption layer 110 , a second adsorption layer 120 , a support layer 130 , a first fixed layer 140 , and a second fixed layer . 150.
  • the first adsorption layer 110 and the second adsorption layer 120 are each a sheet-like structure.
  • the first adsorption layer 110 and the second adsorption layer 120 are both nitrocellulose filter membranes (NC membranes).
  • the nitrocellulose membrane is one of the earliest research microfiltration membranes.
  • the NC membrane is hydrophilic but insoluble in water, soluble in some common organic solvents (acetone, etc.) and slightly soluble in alcohols. It has good properties after film formation, and the NC film has the characteristics of rapid chromatography.
  • the nitrocellulose membrane is selected from the Whatman brand of sterile medical nitrocellulose membranes or non-sterile nitrocellulose membranes of GE Healthcare Life Sciences.
  • the thickness of the first adsorption layer 110 is 100 ⁇ m to 110 ⁇ m, and preferably, the thickness of the first adsorption layer 110 is 100 ⁇ m.
  • the first adsorption layer 110 has a pore diameter of 0.1 ⁇ m to 0.65 ⁇ m.
  • the first adsorption layer 110 has a pore diameter of 0.1 ⁇ m, 0.2 ⁇ m, 0.45 ⁇ m or 0.65 ⁇ m. More preferably, the first adsorption layer 110 has a pore diameter of 0.2 ⁇ m or 0.45 ⁇ m.
  • the thickness of the second adsorption layer 120 is 100 ⁇ m to 110 ⁇ m, and preferably, the thickness of the second adsorption layer 120 is 100 ⁇ m.
  • the second adsorption layer 120 has a pore diameter of 0.1 ⁇ m to 0.65 ⁇ m.
  • the second adsorption layer 120 has a pore diameter of 0.1 ⁇ m, 0.2 ⁇ m, 0.45 ⁇ m or 0.65 ⁇ m. More preferably, the second adsorption layer 120 has a pore diameter of 0.2 ⁇ m or 0.45 ⁇ m.
  • the support layer 130 is interposed between the first adsorption layer 110 and the second adsorption layer 120 such that the first adsorption layer 110 and the second adsorption layer 120 face and are spaced apart.
  • the support layer 130 is also a sheet-like structure, and a sample hole 131 is disposed on the support layer 130, and the sample hole 131 penetrates the support layer 130 such that the first adsorption layer 110 and the second adsorption layer 120 Positive and interval settings.
  • the pore walls of the first adsorption layer 110, the second adsorption layer 120, and the sample well 131 constitute a test site.
  • the sample layer 130 is provided with three spaced sample holes, and the corresponding number of the first adsorption layer 110 and the second adsorption layer 120 are also three, thereby forming three test positions.
  • the above-mentioned sample assembly for terahertz spectroscopy measurement can be used to detect three samples at a time.
  • the number of the sample holes 131, the first adsorption layer 110, and the second adsorption layer 120 may be any other number other than one.
  • the support layer 130 is a highly thermally conductive material. Specifically, the support layer 130 is selected from a copper sheet, an iron sheet, a silver sheet, or a gold sheet. From the viewpoint of test cost, it is preferable that the support layer 130 be a copper sheet or an iron sheet.
  • the support layer 130 has a thickness of 0.2 mm to 0.5 mm.
  • the distance between the first adsorption layer 110 and the second adsorption layer 120 is 0.2 mm to 0.5 mm.
  • the sample hole 131 on the support layer 130 is a circular hole, and the sample hole 131 has a diameter of 5 mm to 12 mm. It can be understood that in other embodiments, the sample well 131 can also be a square hole, or any other irregular shape.
  • the first pinned layer 140 is laminated on the surface of the first adsorbent layer 110 away from the support layer 130.
  • the first pinned layer 140 is in the form of a sheet.
  • the first pinned layer 140 is selected from a copper sheet, an iron sheet, a silver sheet or a gold sheet.
  • the first fixing layer 140 is a copper piece or an iron piece.
  • the first pinned layer 140 has a thickness of 0.2 mm to 0.5 mm.
  • the first test layer 141 is opened on the first fixed layer 140.
  • the first test window 141 penetrates the first fixed layer 140.
  • the first test window 141 is a circular hole, and the first test window 141 has a diameter of 5 mm to 12 mm. It can be understood that in other embodiments, the first test window 141 can also be a square hole, or any other irregular shape.
  • the second pinned layer 150 is laminated on the surface of the second adsorbent layer 120 away from the support layer 130.
  • the second pinned layer 150 is in the form of a sheet.
  • the second pinned layer 150 is selected from a copper sheet, an iron sheet, a silver sheet or a gold sheet.
  • the second pinned layer 150 is a copper piece or an iron piece.
  • the second pinned layer 150 has a thickness of 0.2 mm to 0.5 mm.
  • the second test layer 151 is opened on the second fixed layer 150.
  • the second test window 151 penetrates the second pinned layer 150.
  • the second test window 151 is a circular hole, and the second test window 151 has a diameter of 5 mm to 12 mm. It can be understood that in other embodiments, the second test window 151 can also be a square hole, or any other irregular shape.
  • the first test window 141 and the second test window 151 are both disposed opposite the sample hole 131.
  • the orthographic projections of the first test window opening 141, the second test window 151 and the sample hole 131 on the first adsorption layer 110 can completely coincide.
  • the number of the first test window 141 and the second test window 151 are also three, and three sample holes 131, a first test window 141, and a second test window 151. Three test bits are respectively formed correspondingly. It can be understood that the number of the first test window 141 and the second test window 151 can be any number of not less than one.
  • the above-mentioned sample assembly for terahertz spectroscopy and the terahertz spectroscopy test method use two layers of nitrocellulose membranes spaced apart from each other for adsorption test samples, since the absorption coefficient of the nitrocellulose membrane is similar to that of the polyethylene material. It can be applied to terahertz spectroscopy measurement, and the two layers of nitrocellulose membrane with a spacing of 0.2mm to 0.5mm are combined to form the adsorption layer. Only a small amount of sample solution is added to the adsorption layer, and the solution can be chromatographed to nitrocellulose.
  • the test can be carried out even if the concentration of the sample solution or the amount of the sample is small, and the test can be accurately performed, and the use of the polyethylene powder tableting method or
  • the sample preparation method of the polyvinylidene fluoride film impregnated sample solution requires less sample amount and small sample consumption, and can be applied to detection of less samples.
  • first fixed layer 140 and the second fixed layer 150 may be omitted.
  • an embodiment of the terahertz spectroscopy test method includes the following steps:
  • the sample can be a water soluble good saccharide and other water soluble materials.
  • the sample is added to water and stirred by vortexing until the sample is completely dissolved in the water.
  • the number of revolutions during vortexing is from 2000 rpm to 2500 rpm, and the time of vortexing is from 2 minutes to 3 minutes.
  • the prepared sample solution has a concentration of 0.1 g/mL to 2 g/mL.
  • the terahertz spectrometer is a THz-4000 terahertz spectrometer of Teraview; the terahertz spectral width is 0.06 THz to 4.0 THz; during the scanning of the signal, the scanning range is 0 to 1200 ps, and the acquisition is performed.
  • the rate is 30 scans/s and the resolution of the terahertz spectrum is 1.2 cm -1 .
  • the protective gas is selected from at least one of nitrogen, argon, and helium.
  • the amount of the sample solution dropped onto the sample loading assembly is from 15 ⁇ L to 30 ⁇ L.
  • the sample solution is aspirated by a micropipette and added to the sample loading assembly.
  • the sample solution is dropped to the loading assembly and placed at 15 ° C to 30 ° C for 5 min to 15 min, so that the sample is completely chromatographed into the void of the nitrocellulose membrane.
  • the sample component which is added with the sample solution is tested by a terahertz spectrometer to obtain a sample signal.
  • the terahertz spectrometer is a THz-4000 terahertz spectrometer of Teraview; the terahertz spectral width is 0.06 THz to 4.0 THz; during the scanning of the signal, the scanning range is 0 to 1200 ps, and the acquisition is performed.
  • the rate is 30 scans/s and the resolution of the terahertz spectrum is 1.2 cm -1 .
  • the protective gas is selected from at least one of nitrogen, argon, and helium.
  • the specific steps of obtaining the terahertz absorption spectrum of the sample by the reference signal and the sample signal include:
  • the formula for performing the fast Fourier transform is:
  • ⁇ is the electromagnetic wave frequency
  • t is the time
  • i is the imaginary unit
  • Er(t) is the time domain signal of the reference signal
  • Es(t) is the time domain signal of the sample signal
  • Er( ⁇ ) is the frequency domain of the reference signal
  • the signal, Es( ⁇ ) is the frequency domain signal of the sample signal
  • Ar( ⁇ ) is the amplitude of the electric field of the reference signal
  • As( ⁇ ) is the amplitude of the electric field of the sample signal
  • ⁇ r( ⁇ ) is the phase of the electric field of the reference signal
  • ⁇ s( ⁇ ) is the phase of the electric field of the sample signal.
  • the data processing model based on the Fresnel formula is used to extract the refractive index n( ⁇ ) and the absorption coefficient ⁇ ( ⁇ ) of the sample.
  • the formula of the data processing model based on the Fresnel formula is:
  • ⁇ ( ⁇ ) is the ratio of the amplitudes of the sample signal and the reference signal
  • ⁇ ( ⁇ ) is the phase difference between the sample signal and the reference signal
  • d is the thickness of the sample
  • c is the propagation velocity of the electromagnetic wave in vacuum.
  • the above terahertz spectroscopy test method uses two layers of nitrocellulose membranes arranged at intervals to adsorb test samples, which can be applied to tests with fewer samples, high test accuracy and low detection limit.
  • sample loading assembly for terahertz spectroscopy and the terahertz spectroscopy test method will be described below in conjunction with specific embodiments.
  • (1) preparing a sample assembly A for terahertz spectroscopy comprising: a first adsorption layer, a second adsorption layer, a support layer sandwiched between the first adsorption layer and the second adsorption layer, the first adsorption layer being far away a surface of the support layer is laminated with a first fixed layer, and a second fixed layer is stacked on the surface of the second adsorption layer away from the support layer; a sample hole is formed on the support layer, a first test window is opened on the first fixed layer, and a second test layer is opened on the second fixed layer Two test windows.
  • the support layer, the first fixed layer and the second fixed layer are both copper sheets having a thickness of 0.32 mm
  • the first adsorption layer and the second adsorption layer are both nitrocellulose membranes having a thickness of 100 ⁇ m and a pore diameter of 0.1 ⁇ m
  • samples The hole, the first test window and the second test window are all circular holes with a diameter of 8 mm
  • the sample hole, the first test window and the second test window are directly opposite each other.
  • the support layer, the first fixed layer and the second fixed layer are both copper sheets having a thickness of 0.32 mm
  • the first adsorption layer and the second adsorption layer are both nitrocellulose membranes having a thickness of 100 ⁇ m and a pore diameter of 0.2 ⁇ m
  • samples The hole, the first test window and the second test window are all circular holes with a diameter of 8 mm
  • the sample hole, the first test window and the second test window are directly opposite each other.
  • (3) preparing a sample assembly C for terahertz spectroscopy comprising: a first adsorption layer, a second adsorption layer, a support layer sandwiched between the first adsorption layer and the second adsorption layer, the first adsorption layer being far away a surface of the support layer is laminated with a first fixed layer, and a second fixed layer is stacked on the surface of the second adsorption layer away from the support layer; a sample hole is formed on the support layer, a first test window is opened on the first fixed layer, and a second test layer is opened on the second fixed layer Two test windows.
  • the support layer, the first fixed layer and the second fixed layer are both copper sheets having a thickness of 0.32 mm
  • the first adsorption layer and the second adsorption layer are both nitrocellulose membranes having a thickness of 100 ⁇ m and a pore diameter of 0.45 ⁇ m
  • samples The hole, the first test window and the second test window are all circular holes with a diameter of 8 mm, and the sample hole, the first test window and the second test window are directly opposite each other.
  • a sample assembly D for terahertz spectroscopy was prepared, and 22 mg of polyethylene was accurately weighed to form a disk having a diameter of 13 mm and a thickness of 0.2 mm by tableting.
  • the nitrocellulose membrane has a similar absorption coefficient to polyethylene and is suitable for use as a fixed medium for sample testing.
  • (1) acquiring four sample loading components comprising: a first adsorption layer, a second adsorption layer, a support layer sandwiched between the first adsorption layer and the second adsorption layer, and the first adsorption layer is laminated on a surface away from the support layer
  • the first fixed layer, the second adsorption layer is stacked on the surface of the support layer, and the second fixed layer is formed on the support layer; the first test window is opened on the first fixed layer, and the second test window is opened on the second fixed layer.
  • the support layer, the first fixed layer and the second fixed layer are both copper sheets having a thickness of 0.5 mm
  • the first adsorption layer and the second adsorption layer are both nitrocellulose membranes having a thickness of 100 ⁇ m and a pore diameter of 0.2 ⁇ m
  • samples The hole, the first test window and the second test window are all circular holes having a diameter of 12 mm, and the sample hole, the first test window and the second test window are directly disposed.
  • the sample assembly E, the sample assembly F, the sample assembly G, the sample assembly H, and the sample assembly I are placed in a terahertz spectrometer for testing to obtain a terahertz absorption spectrum, as shown in FIG.
  • (1) acquiring four sample loading components comprising: a first adsorption layer, a second adsorption layer, a support layer sandwiched between the first adsorption layer and the second adsorption layer, and the first adsorption layer is laminated on a surface away from the support layer
  • the first fixed layer, the second adsorption layer is stacked on the surface of the support layer, and the second fixed layer is formed on the support layer; the first test window is opened on the first fixed layer, and the second test window is opened on the second fixed layer.
  • the support layer, the first fixed layer and the second fixed layer are both copper sheets having a thickness of 0.2 mm, and the first adsorption layer and the second adsorption layer are both nitrocellulose membranes having a thickness of 100 ⁇ m and a pore diameter of 0.045 ⁇ m.
  • the sample hole, the first test window and the second test window are all circular holes having a diameter of 10 mm, and the sample hole, the first test window and the second test window are directly disposed.
  • the sample assembly J, the sample assembly K, the sample assembly L, the sample assembly M, and the sample assembly N are placed in a terahertz spectrometer for testing to obtain a terahertz absorption spectrum, as shown in FIG.
  • the fructose characteristic absorption peaks are 1.68T (56cm -1 ) and 2.12T (71cm -1 ), and the absorption intensity of the sample gradually increases with the increase of mass. .

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Abstract

一种用于太赫兹光谱测量的载样组件(100)，该太赫兹光谱测量的载样组件（100）包括：支撑层（130）、第一吸附层（110）及第二吸附层（120）；支撑层（130）夹设于第一吸附层（110）与第二吸附层（120）之间，使得第一吸附层（110）与第二吸附层（120）形成0.2mm～0.5mm的间隙；第一吸附层（110）及第二吸附层（120）均为硝酸纤维素膜。一种采用所述太赫兹光谱测量载样组件(100)用于太赫兹光谱的测试方法。

Description

用于太赫兹光谱测量的载样组件及太赫兹光谱测试方法 技术领域
本发明涉及太赫兹检测技术领域，特别是涉及用于太赫兹光谱测量的载样组件及太赫兹光谱测试方法。
背景技术
太赫兹波是指频率在0.1-10THz范围内的电磁波，介于微波和红外之间，由于太赫兹波对有机分子的振动和转动模式敏感，物质的太赫兹光谱具有指纹性，而且太赫兹时域光谱技术可以同时获得幅值和相位信息，信息量丰富，且物质的太赫兹光谱是物质的指纹光谱，具有高透性、低能性、瞬时性、敏锐性、高信噪比等特点，这些特点使得太赫兹无损检测技术在生物医学、生物化学、化工等很多方法有很重要的应用。
目前，太赫兹技术制备样本的方法主要是粉末压片法，该方法虽然操作简单，但粉碎过筛步骤所耗时间较长，并且压片所需样本量较大，至少需要10mg样本与聚乙烯混匀压片(直径为13mm圆型薄片)，对于微量样本压片法不太适合。
用于液体样品的太赫兹技术检测方法主要是液体池法。2013年10月2日公开的，公开号为103335976A的“利用太赫兹时域光谱检测硫酸盐、硝酸盐溶液浓度的方法”专利，使用的是聚乙烯液体池，检测样品浓度为4％-18％；2014年1月1日公开的，公开号为103487399A的“一种定量检测盐酸四环素溶液的太赫兹时域光谱方法”专利，使用的是聚偏二氟乙烯薄膜，样品检测方法为聚偏二氟乙烯薄膜浸入到溶液样品中，检测样品浓度为0.01％-2％。综上可知，目前常用的太赫兹检测中制样方法局限，所需样品较多，样品耗用量大，不适用于样品较少时的检测。
发明内容
基于此，有必要针对目前常用的太赫兹检测中制样方法局限，所需样品较多，样品耗用量大的问题，提供一种用于太赫兹光谱测量的载样组件及太赫兹光谱测试方法。
一种用于太赫兹光谱测量的载样组件，包括：支撑层、第一吸附层及第二吸附层；
所述支撑层夹设于所述第一吸附层与所述第二吸附层之间，使得所述第一吸附层与所述第二吸附层形成0.2mm～0.5mm的间隙；
所述第一吸附层及所述第二吸附层均为硝酸纤维素膜。
在其中一个实施方式中，所述支撑层上开设有样品孔使得所述第一吸附层与所述第二吸附层间隔设置。
在其中一个实施方式中，还包括第一固定层及第二固定层，所述第一固定层层叠于所述第一吸附层远离所述支撑层的表面，所述第二固定层层叠于所述第二吸附层远离所述支撑层的表面；
所述第一固定层上开设有第一测试窗口，所述第二固定层上开设有第二测试窗口，所述第一测试窗口与所述第二测试窗口均与所述样品孔相通。
在其中一个实施方式中，所述第一固定层及所述第二固定层的厚度分别为0.2mm～0.5mm。
在其中一个实施方式中，所述硝酸纤维素膜的孔径为0.1μm～0.65μm。
在其中一个实施方式中，所述支撑层为铜片、铁片、银片或金片。
一种太赫兹光谱测试方法，包括以下步骤：
称取样品加入到水中配制得到样品溶液；
将上述的载样组件放置于太赫兹光谱仪中，并在保护性气体的吹扫下，进行空载测试得到参考信号；
将所述样品溶液滴加到所述载样组件上；
在保护性气体的吹扫下，采用太赫兹光谱仪对滴加有样品溶液的所述载样组件进行测试得到样品信号；
通过所述参考信号及所述样品信号得到所述样品的太赫兹吸收谱。
在其中一个实施方式中，所述太赫兹光谱仪为Teraview公司的型号为 TPS-4000的太赫兹光谱仪；太赫兹谱宽为0.06THz～4.0THz；在信号的扫描过程中，扫描范围为0～1200ps，采集速率为30scans/s，太赫兹光谱的分辨率为1.2cm -1。
在其中一个实施方式中，所述通过所述参考信号及所述样品信号得到所述样品的太赫兹吸收谱的步骤包括：
将所述参考信号及所述样品信号的时域谱进行快速傅里叶变换得到所述参考信号及所述样品信号的频域谱，进行所述快速傅里叶变换的公式为：
Er(ω)＝Ar(ω)exp[-iφr(ω)]＝∫Er(t)exp(-iωt)dt；
Es(ω)＝As(ω)exp[-iφs(ω)]＝∫Es(t)exp(-iωt)dt；
其中，ω为电磁波频率，t为时间，i为虚数单位；Er(t)为参考信号的时域信号，Es(t)为样品信号的时域信号；Er(ω)为参考信号的频域信号，Es(ω)为样品信号的频域信号；Ar(ω)为参考信号电场的振幅，As(ω)为样品信号电场的振幅；φr(ω)为参考信号电场的相位，φs(ω)为样品信号电场的相位；
再采用基于菲涅尔公式的数据处理模型提取样品的折射率n(ω)和吸收系数α(ω)，所述基于菲涅尔公式的数据处理模型的公式为：
Figure PCTCN2018080594-appb-000001
Figure PCTCN2018080594-appb-000002
其中，ρ(ω)为样品信号和参考信号的振幅的比值，φ(ω)为样品信号和参考信号的相位差，d为样品的厚度，c为电磁波在真空中的传播速度。
在其中一个实施方式中，所述样品溶液的浓度为0.1g/mL～2g/mL。
上述用于太赫兹光谱测量的载样组件及太赫兹光谱测试方法采用两层相互间隔设置的硝酸纤维素膜用于吸附测试样品，由于硝酸纤维素膜的吸收系数与聚乙烯材料的吸收系数相近，完全可以适用于太赫兹光谱测量，且采用两层间距为0.2mm～0.5mm的硝酸纤维素膜组合形成吸附层只需在吸附层上滴加少量样品溶液，样品溶液即可层析到硝酸纤维素膜的孔隙之间或两层硝酸纤维素膜的间隙之间，即可进行测试，即使样品溶液的浓度或样品量较少，也能准确进行测试，并避免了使用聚乙烯粉末压片法或者聚偏二氟乙烯薄膜浸渍样品溶液的 制样方法，所需样品量少，样品耗用小，可以适用于较少样品时的检测。
附图说明
图1为一实施方式的用于太赫兹光谱测量的载样组件的剖视图；
图2为图1所示的用于太赫兹光谱测量的载样组件的分解示意图；
图3为一实施方式的太赫兹光谱测试方法的流程图；
图4为样品组件A、样品组件B、样品组件C及样品组件D的太赫兹吸收谱；
图5为样品组件E、样品组件F、样品组件G、样品组件H及样品组件I的太赫兹吸收谱；
图6为样品组件J、样品组件K、样品组件L、样品组件M及样品组件N的太赫兹吸收谱。
具体实施方式
下面将结合具体实施方式及附图对用于太赫兹光谱测量的载样组件及太赫兹光谱测试方法做进一步的详细说明。
请参阅图1及图2，一实施方式的用于太赫兹光谱测量的载样组件100包括第一吸附层110、第二吸附层120、支撑层130、第一固定层140及第二固定层150。
在图示的实施方式中，第一吸附层110与第二吸附层120分别为片状结构。第一吸附层110及第二吸附层120均为硝酸纤维素膜(nitrocellulose filter membrane，简称NC膜)。
硝酸纤维素膜是人们最早开始研究的一种微滤膜。NC膜具有一定亲水性但却不溶于水，可溶于一些比较常见的有机溶剂(丙酮等)，并微溶于醇类。成膜后其具有良好的性能，NC膜具有快速层析的特性。
在其中一个实施方式中，硝酸纤维素膜选自GE医疗生命科学的Whatman品牌的无菌型硝酸纤维素膜或非无菌型硝酸纤维素膜。
在其中一个实施方式中，第一吸附层110的厚度为100μm～110μm，优选的， 第一吸附层110的厚度为100μm。第一吸附层110的孔径为0.1μm～0.65μm。优选的，第一吸附层110的孔径为0.1μm、0.2μm、0.45μm或0.65μm。更优选的，第一吸附层110的孔径为0.2μm或0.45μm。
在其中一个实施方式中，第二吸附层120的厚度为100μm～110μm，优选的，第二吸附层120的厚度为100μm。第二吸附层120的孔径为0.1μm～0.65μm。优选的，第二吸附层120的孔径为0.1μm、0.2μm、0.45μm或0.65μm。更优选的，第二吸附层120的孔径为0.2μm或0.45μm。
在图示的实施方式中，第一吸附层110与第二吸附层120之间夹设有支撑层130，从而使得第一吸附层110与第二吸附层120正对且间隔设置。
进一步的，在图示的实施方式中，支撑层130也为片状结构，在支撑层130上设有样品孔131，样品孔131贯穿支撑层130使得第一吸附层110与第二吸附层120正对且间隔设置。第一吸附层110、第二吸附层120及样品孔131的孔壁组成一个测试位。
在图示的实施方式中，支撑层130上开设有3个间隔设置的样品孔，对应的第一吸附层110和第二吸附层120的数量也分别为3个，从而组成3个测试位，使得上述用于太赫兹光谱测量的载样组件一次可以进行3个样品的检测。当然，在其他实施方式中，样品孔131、第一吸附层110及第二吸附层120的数量也可以是其他不少于1的任意数量。
在其中一个实施方式中，支撑层130为高导热材料，具体的，支撑层130选自铜片、铁片、银片或金片。从测试成本考虑，优选的，支撑层130为铜片或铁片。
在其中一个实施方式中，支撑层130的厚度为0.2mm～0.5mm。
进一步的，使得第一吸附层110与第二吸附层120的间距为0.2mm～0.5mm。
在其中一个实施方式中，支撑层130上的样品孔131为圆孔，样品孔131的直径为5mm～12mm。可以理解的是，在其他实施方式中，样品孔131也可以为方孔，或者其他任意不规则形状。
在图示的实施方式中，第一固定层140层叠于第一吸附层110远离支撑层130的表面。
在其中一个实施方式中，第一固定层140为片状。第一固定层140选自铜片、铁片、银片或金片。优选的，第一固定层140为铜片或铁片。
在其中一个实施方式中，第一固定层140的厚度为0.2mm～0.5mm。
在其中一个实施方式中，第一固定层140上开设有第一测试窗口141。第一测试窗口141贯穿第一固定层140。在图示的实施方式中，第一测试窗口141为圆孔，第一测试窗口141的直径为5mm～12mm。可以理解的是，在其他实施方式中，第一测试窗口141也可以为方孔，或者其他任意不规则形状。
在其中一个实施方式中，第二固定层150层叠于第二吸附层120远离支撑层130的表面。
在其中一个实施方式中，第二固定层150为片状。第二固定层150选自铜片、铁片、银片或金片。优选的，第二固定层150为铜片或铁片。
在其中一个实施方式中，第二固定层150的厚度为0.2mm～0.5mm。
在其中一个实施方式中，第二固定层150上开设有第二测试窗口151。第二测试窗口151贯穿第二固定层150。在图示的实施方式中，第二测试窗口151为圆孔，第二测试窗口151的直径为5mm～12mm。可以理解的是，在其他实施方式中，第二测试窗口151也可以为方孔，或者其他任意不规则形状。
在图示的实施方式中，第一测试窗口141、第二测试窗口151均与样品孔131正对设置。优选的，第一测试窗口口141、第二测试窗口151及样品孔131在第一吸附层110上的正投影能够完全重合。
需要说明的是，在图示的实施方式中，第一测试窗口141和第二测试窗口151的数量也分别为3个，且三个样品孔131、第一测试窗口141及第二测试窗口151分别对应形成三个测试位。可以理解的是，第一测试窗口141和第二测试窗口151的数量可以是不少于一个的任意数量。
上述用于太赫兹光谱测量的载样组件及太赫兹光谱测试方法采用两层相互间隔设置的硝酸纤维素膜用于吸附测试样品，由于硝酸纤维素膜的吸收系数与聚乙烯材料的吸收系数相近，完全可以适用于太赫兹光谱测量，且采用两层间距为0.2mm～0.5mm的硝酸纤维素膜组合形成吸附层只需在吸附层上滴加少量样品溶液，溶液即可层析到硝酸纤维素膜的空隙之间或两层硝酸纤维素膜的间隙 之间，即可进行测试，即使样品溶液的浓度或样品量较少，均能准确进行测试，并避免了使用聚乙烯粉末压片法或者聚偏二氟乙烯薄膜浸渍样品溶液的制样方法，所需样品量少，样品耗用小，可以适用于较少样品时的检测。
需要说明的是，在其他实施方式中，第一固定层140及第二固定层150均可以省略。
请进一步参阅图3，一实施方式的太赫兹光谱测试方法包括以下步骤：
S110、称取样品加入到水中配制得到样品溶液。
在其中一个实施方式中，样品可以为水溶性好的糖类及其他水溶性物质。
在其中一个实施方式中，将样品加入到水中后采用涡旋搅拌的方式搅拌至样品完全溶解在水中。涡旋搅拌时的转速为2000rpm～2500rpm，涡旋的时间为2min～3min。
在其中一个实施方式中，制备的样品溶液的浓度为0.1g/mL～2g/mL。
S120、将载样组件放置于太赫兹光谱仪中，并在保护性气体的吹扫下，进行空载测试得到参考信号。
在其中一个实施方式中，太赫兹光谱仪为Teraview公司的型号为TPS-4000的太赫兹光谱仪；太赫兹谱宽为0.06THz～4.0THz；在信号的扫描过程中，扫描范围为0～1200ps，采集速率为30scans/s，太赫兹光谱的分辨率为1.2cm -1。
在其中一个实施方式中，保护性气体选自氮气、氩气和氦气中的至少一种。
S130、将样品溶液滴加到载样组件上。
在其中一个实施方式中，滴加到载样组件上的样品溶液的用量为15μL～30μL。优选的，采用微量移液器吸取样品溶液滴加到载样组件上。
在其中一个实施方式中，样品溶液滴加到载样组件后在15℃～30℃下放置5min～15min，使得样品完全层析到硝酸纤维素膜的空隙中。
S140、在保护性气体的吹扫下，采用太赫兹光谱仪对滴加有样品溶液的载样组件进行测试得到样品信号。
在其中一个实施方式中，太赫兹光谱仪为Teraview公司的型号为TPS-4000的太赫兹光谱仪；太赫兹谱宽为0.06THz～4.0THz；在信号的扫描过程中，扫描范围为0～1200ps，采集速率为30scans/s，太赫兹光谱的分辨率为1.2cm -1。
在其中一个实施方式中，保护性气体选自氮气、氩气和氦气中的至少一种。
S150、通过参考信号及样品信号得到样品的太赫兹吸收谱。
在其中一个实施方式中，通过参考信号及样品信号得到样品的太赫兹吸收谱的具体步骤包括：
S151、将参考信号及样品信号的时域谱进行快速傅里叶变换得到参考信号及样品信号的频域谱。
在其中一个实施方式中，进行快速傅里叶变换的公式为：
Er(ω)＝Ar(ω)exp[-iφr(ω)]＝∫Er(t)exp(-iωt)dt；
Es(ω)＝As(ω)exp[-iφs(ω)]＝∫Es(t)exp(-iωt)dt；
其中，ω为电磁波频率，t为时间，i为虚数单位；Er(t)为参考信号的时域信号，Es(t)为样品信号的时域信号；Er(ω)为参考信号的频域信号，Es(ω)为样品信号的频域信号；Ar(ω)为参考信号电场的振幅，As(ω)为样品信号电场的振幅；φr(ω)为参考信号电场的相位，φs(ω)为样品信号电场的相位。
S152、再采用基于菲涅尔公式的数据处理模型提取样品的折射率n(ω)和吸收系数α(ω)。
在其中一个实施方式中，基于菲涅尔公式的数据处理模型的公式为：
Figure PCTCN2018080594-appb-000003
Figure PCTCN2018080594-appb-000004
其中，ρ(ω)为样品信号和参考信号的振幅的比值，φ(ω)为样品信号和参考信号的相位差，d为样品的厚度，c为电磁波在真空中的传播速度。
上述太赫兹光谱测试方法，采用两层相互间隔设置的硝酸纤维素膜用于吸附测试样品，能够适用于较少样品时的检测，测试准确度高，检出限低。
下面将结合具体实施例对用于太赫兹光谱测量的载样组件及太赫兹光谱测试方法进行说明。
实施例1
(1)制备用于太赫兹光谱测试的样品组件A，包括：第一吸附层、第二吸附 层、夹设于第一吸附层与第二吸附层之间的支撑层，第一吸附层远离支撑层的表面层叠第一固定层，第二吸附层远离支撑层的表面层叠第二固定层；支撑层上开设样品孔，第一固定层上开设第一测试窗口，第二固定层上开设第二测试窗口。
其中，支撑层、第一固定层及第二固定层均为厚度为0.32mm的铜片，第一吸附层及第二吸附层均为厚度为100μm，孔径为0.1μm的硝酸纤维素膜，样品孔、第一测试窗口及第二测试窗口均为直径为8mm的圆孔，且样品孔、第一测试窗口及第二测试窗口正对设置。
(2)制备用于太赫兹光谱测试的样品组件B，包括：第一吸附层、第二吸附层、夹设于第一吸附层与第二吸附层之间的支撑层，第一吸附层远离支撑层的表面层叠第一固定层，第二吸附层远离支撑层的表面层叠第二固定层；支撑层上开设样品孔，第一固定层上开设第一测试窗口，第二固定层上开设第二测试窗口。
其中，支撑层、第一固定层及第二固定层均为厚度为0.32mm的铜片，第一吸附层及第二吸附层均为厚度为100μm，孔径为0.2μm的硝酸纤维素膜，样品孔、第一测试窗口及第二测试窗口均为直径为8mm的圆孔，且样品孔、第一测试窗口及第二测试窗口正对设置。
(3)制备用于太赫兹光谱测试的样品组件C，包括：第一吸附层、第二吸附层、夹设于第一吸附层与第二吸附层之间的支撑层，第一吸附层远离支撑层的表面层叠第一固定层，第二吸附层远离支撑层的表面层叠第二固定层；支撑层上开设样品孔，第一固定层上开设第一测试窗口，第二固定层上开设第二测试窗口。
其中，支撑层、第一固定层及第二固定层均为厚度为0.32mm的铜片，第一吸附层及第二吸附层均为厚度为100μm，孔径为0.45μm的硝酸纤维素膜，样品孔、第一测试窗口及第二测试窗口均为直径为8mm的圆孔，且样品孔、第一测试窗口及第二测试窗口正对设置。
(4)制备用于太赫兹光谱测试的样品组件D，准确称取22mg的聚乙烯通过压片形成直径为13mm，厚度为0.2mm的圆片。
(5)将样品组件A、样品组件B、样品组件C及样品组件D放入太赫兹光谱仪中进行测试得到太赫兹吸收谱，如图4所示。
从图4可以看出，硝酸纤维素膜与聚乙烯的吸收系数接近，适用于作为样品测试的固定介质。
实施例2
(1)获取四个载样组件，包括：第一吸附层、第二吸附层、夹设于第一吸附层与第二吸附层之间的支撑层，第一吸附层远离支撑层的表面层叠第一固定层，第二吸附层远离支撑层的表面层叠第二固定层；支撑层上开设样品孔，第一固定层上开设第一测试窗口，第二固定层上开设第二测试窗口。
其中，支撑层、第一固定层及第二固定层均为厚度为0.5mm的铜片，第一吸附层及第二吸附层均为厚度为100μm，孔径为0.2μm的硝酸纤维素膜，样品孔、第一测试窗口及第二测试窗口均为直径为12mm的圆孔，且样品孔、第一测试窗口及第二测试窗口正对设置。
(2)精确称取2mg的一水葡萄糖，向其中加入20μL的去离子水混匀并涡旋30s制备得到浓度为0.1g/mL的样品溶液，用微量移液器吸取20μL的样品溶液滴加到一个载样组件上得到样品组件E。
(3)精确称取4mg的一水葡萄糖，向其中加入20μL的去离子水混匀并涡旋30s制备得到浓度为0.2g/mL的样品溶液，用微量移液器吸取20μL的样品溶液滴加到一个载样组件上得到样品组件F。
(4)精确称取6mg的一水葡萄糖，向其中加入20μL的去离子水混匀并涡旋30s制备得到浓度为0.3g/mL的样品溶液，用微量移液器吸取20μL的样品溶液滴加到一个载样组件上得到样品组件G。
(5)精确称取8mg的一水葡萄糖，向其中加入20μL的去离子水混匀并涡旋30s制备得到浓度为0.4g/mL的样品溶液，用微量移液器吸取20μL的样品溶液滴加到一个载样组件上得到样品组件H。
(6)精确称取25mg的一水葡萄糖并加入175mg的聚乙烯混合均匀后通过压片形成直径为13mm，厚度为1.5mm的圆片，得到样品组件I。
(7)将样品组件E、样品组件F、样品组件G、样品组件H及样品组件I放入太赫兹光谱仪中进行测试得到太赫兹吸收谱，如图5所示。
从图5可以看出NC膜测试结果与压片结果一致，一水葡萄糖特征吸收峰均为1.80T(59cm -1)、1.98T(65cm -1)、2.36T(78cm -1)、2.42T(81cm -1)，样品吸收强度随着质量的增加逐渐提高。
实施例3
(1)获取四个载样组件，包括：第一吸附层、第二吸附层、夹设于第一吸附层与第二吸附层之间的支撑层，第一吸附层远离支撑层的表面层叠第一固定层，第二吸附层远离支撑层的表面层叠第二固定层；支撑层上开设样品孔，第一固定层上开设第一测试窗口，第二固定层上开设第二测试窗口。
其中，支撑层、第一固定层及第二固定层均为厚度为0.2mm的铜片，第一吸附层及第二吸附层均为厚度为100μm，孔径为0.0.45μm的硝酸纤维素膜，样品孔、第一测试窗口及第二测试窗口均为直径为10mm的圆孔，且样品孔、第一测试窗口及第二测试窗口正对设置。
(2)精确称取2mg的果糖，向其中加入20μL的去离子水混匀并涡旋30s制备得到浓度为0.1g/mL的样品溶液，用微量移液器吸取20μL的样品溶液滴加到一个载样组件上得到样品组件J。
(3)精确称取4mg的果糖，向其中加入20μL的去离子水混匀并涡旋30s制备得到浓度为0.2g/mL的样品溶液，用微量移液器吸取20μL的样品溶液滴加到一个载样组件上得到样品组件K。
(4)精确称取6mg的果糖，向其中加入20μL的去离子水混匀并涡旋30s制备得到浓度为0.3g/mL的样品溶液，用微量移液器吸取20μL的样品溶液滴加到一个载样组件上得到样品组件L。
(5)精确称取8mg的果糖，向其中加入20μL的去离子水混匀并涡旋30s制备得到浓度为0.4g/mL的样品溶液，用微量移液器吸取20μL的样品溶液滴加到一个载样组件上得到样品组件M。
(6)精确称取50mg的果糖并加入150mg的聚乙烯混合均匀后通过压片形成 直径为13mm，厚度为1.5mm的圆片，得到样品组件N。
(7)将样品组件J、样品组件K、样品组件L、样品组件M及样品组件N放入太赫兹光谱仪中进行测试得到太赫兹吸收谱，如图6所示。
从图6可以看出NC膜测试结果与也与压片结果一致，果糖特征吸收峰均为1.68T(56cm -1)、2.12T(71cm -1)，样品吸收强度随着质量的增加逐渐提高。
以上所述实施例的各技术特征可以进行任意的组合，为使描述简洁，未对上述实施例中的各个技术特征所有可能的组合都进行描述，然而，只要这些技术特征的组合不存在矛盾，都应当认为是本说明书记载的范围。
以上所述实施例仅表达了本发明的几种实施方式，其描述较为具体和详细，但并不能因此而理解为对发明专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进，这些都属于本发明的保护范围。因此，本发明专利的保护范围应以所附权利要求为准。

Claims (10)

  1. 一种用于太赫兹光谱测量的载样组件，其特征在于，包括：支撑层、第一吸附层及第二吸附层；
    所述支撑层夹设于所述第一吸附层与所述第二吸附层之间，使得所述第一吸附层与所述第二吸附层形成0.2mm～0.5mm的间隙；
    所述第一吸附层及所述第二吸附层均为硝酸纤维素膜。
  2. 根据权利要求1所述的用于太赫兹光谱测量的载样组件，其特征在于，所述支撑层上开设有样品孔使得所述第一吸附层与所述第二吸附层间隔设置。
  3. 根据权利要求2所述的用于太赫兹光谱测量的载样组件，其特征在于，还包括第一固定层及第二固定层，所述第一固定层层叠于所述第一吸附层远离所述支撑层的表面，所述第二固定层层叠于所述第二吸附层远离所述支撑层的表面；
    所述第一固定层上开设有第一测试窗口，所述第二固定层上开设有第二测试窗口，所述第一测试窗口与所述第二测试窗口均与所述样品孔相通。
  4. 根据权利要求3所述的用于太赫兹光谱测量的载样组件，其特征在于，所述第一固定层及所述第二固定层的厚度分别为0.2mm～0.5mm。
  5. 根据权利要求1所述的用于太赫兹光谱测量的载样组件，其特征在于，所述硝酸纤维素膜的孔径为0.1μm～0.65μm。
  6. 根据权利要求1所述的用于太赫兹光谱测量的载样组件，其特征在于，所述支撑层为铜片、铁片、银片或金片。
  7. 一种太赫兹光谱测试方法，其特征在于，包括以下步骤：
    称取样品加入到水中配制得到样品溶液；
    将如权利要求1～6任一项所述的载样组件放置于太赫兹光谱仪中，并在保护性气体的吹扫下，进行空载测试得到参考信号；
    将所述样品溶液滴加到所述载样组件上；
    在保护性气体的吹扫下，采用太赫兹光谱仪对滴加有样品溶液的所述载样组件进行测试得到样品信号；
    通过所述参考信号及所述样品信号得到所述样品的太赫兹吸收谱。
  8. 根据权利要求7所述的太赫兹光谱测试方法，其特征在于，所述太赫兹光谱仪为Teraview公司的型号为TPS-4000的太赫兹光谱仪；太赫兹谱宽为0.06THz～4.0THz；在信号的扫描过程中，扫描范围为0～1200ps，采集速率为30scans/s，太赫兹光谱的分辨率为1.2cm-1。
  9. 根据权利要求7所述的太赫兹光谱测试方法，其特征在于，所述通过所述参考信号及所述样品信号得到所述样品的太赫兹吸收谱的步骤包括：
    将所述参考信号及所述样品信号的时域谱进行快速傅里叶变换得到所述参考信号及所述样品信号的频域谱，进行所述快速傅里叶变换的公式为：
    Er(ω)＝Ar(ω)exp[-iφr(ω)]＝∫Er(t)exp(-iωt)dt；
    Es(ω)＝As(ω)exp[-iφs(ω)]＝∫Es(t)exp(-iωt)dt；
    其中，ω为电磁波频率，t为时间，i为虚数单位；Er(t)为参考信号的时域信号，Es(t)为样品信号的时域信号；Er(ω)为参考信号的频域信号，Es(ω)为样品信号的频域信号；Ar(ω)为参考信号电场的振幅，As(ω)为样品信号电场的振幅；φr(ω)为参考信号电场的相位，φs(ω)为样品信号电场的相位；
    再采用基于菲涅尔公式的数据处理模型提取样品的折射率n(ω)和吸收系数α(ω)，所述基于菲涅尔公式的数据处理模型的公式为：
    Figure PCTCN2018080594-appb-100001
    Figure PCTCN2018080594-appb-100002
    其中，ρ(ω)为样品信号和参考信号的振幅的比值，φ(ω)为样品信号和参考信号的相位差，d为样品的厚度，c为电磁波在真空中的传播速度。
  10. 根据权利要求7所述的太赫兹光谱测试方法，其特征在于，所述样品溶液的浓度为0.1g/mL～2g/mL。
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