WO2019051739A1

WO2019051739A1 - Preparation method for effective part of patrinia villosa juss

Info

Publication number: WO2019051739A1
Application number: PCT/CN2017/101796
Authority: WO
Inventors: 覃华贤
Original assignee: 覃华贤
Priority date: 2017-09-14
Filing date: 2017-09-15
Publication date: 2019-03-21

Abstract

A preparation method for an effective part of Patrinia villosa juss. The preparation method comprises the following steps: performing reflux extraction on Patrinia villosa juss 2 to 4 times with ethanol having a volume fraction of 45-55% for 1-3 h each time; combining extracting solutions; concentrating the extracting solutions under reduced pressure to obtain an extract; dissolving the extract in an appropriate amount of water, and then performing chromatographic separation with a macroporous resin adsorption column; performing gradient elution by sequentially using pure water and ethanol having the volume fractions of 10%, 30%, 50%, and 95%; collecting a part eluted with 50% ethanol; performing a second chromatographic separation with a silica gel column; eluting by sequentially using chloroform-methanol having the volume ratios of 40:1 and 20:1; collecting a part eluted by 20:1 chloroform-methanol; and performing concentration, thereby obtaining an effective part of Patrinia villosa juss. The preparation method for an effective part of Patrinia villosa juss is easy to operate. The obtained extract is rich in lignin compounds and has various pharmacological activities, and thus can be used as an effective part of Patrinia villosa juss for medicine preparation.

Description

Method for preparing effective part of white flower

Technical field

The invention belongs to the field of medicine, and particularly relates to a preparation method of an effective part of the white flower.

Background technique

The white flower is a kind of genus of the genus Sauce. Herbs annual, up to 1 m, stems erect, whole plants begin to have inverted white coarse hairs, gradually smooth, only a few leaves at the nodes, the upper part is less branched. Leaves opposite, few sessile, narrowly elliptic, both sides Swollen, apex long pointed or pointed, margin serrate, base narrow and narrow, sessate and stalked, flowers white, flowering from May to June. Distributed in Jilin Province, Liaoning Province, Shanxi Province, Jiangsu Province, Anhui Province. White flower succulent grass is preferred in a humid and slightly yin environment, which is more resistant to cold. It is usually born in the creeks of 50-2000 meters above sea level, under the hillsides, forest margins, roadsides, shrubs and grasses. The soil requirements are not very strict, but fertile sandy loam is preferred.

White flower defeated the whole herb into the medicine. Sexual taste bitter, cold, non-toxic; efficacy dilated swelling, blood stasis and excretion. Treatment of intestinal fistula has pus, blood gas, abdominal pain, eye red diaphragm and sputum; treatment of appendicitis, dysentery, hepatitis, tonsil Inflammation, swollen sores and other symptoms.

Summary of the invention

It is an object of the present invention to provide a method for preparing an effective part of P. sinensis.

The invention is achieved by the following technical solutions:

The invention relates to a method for preparing an effective part of the white flower, which comprises the following steps: refluxing the white flower of the succulent grass with a volume fraction of 45-55% ethanol for 2-4 times, each time 1-3 hours, combining the extracts, and extracting the extract. The extract is concentrated to obtain an extract; the extract is dissolved in an appropriate amount of water, and then separated by macroporous adsorption resin column chromatography, followed by elution with pure water, a volume fraction of 10%, 30%, 50%, 95% ethanol, and 50% ethanol is collected. The eluted fraction was separated by silica gel column chromatography, and eluted with chloroform-methanol in a volume ratio of 40:1 and 20:1, and a 20:1 fraction was collected and concentrated.

Preferably, in the preparation method, the white flower is extracted by refluxing with a volume fraction of 50% ethanol.

Preferably, in the preparation method, the hot reflux is extracted 3 times.

Preferably, in the preparation method, each hot reflux is extracted for 2 hours.

Preferably, in the preparation method, 1 kg of succulent grass is subjected to hot reflux extraction using 4-6 L of solvent.

Preferably, in the preparation method, the extract is dissolved in an appropriate amount of water, filtered with cotton, and separated by macroporous adsorption resin column chromatography.

Preferably, in the preparation method, 1 g of the extract is dissolved with 4 to 6 mL of water.

Preferably, in the preparation method, the macroporous resin is of the D101 type.

The technical effect of the invention:

The preparation method of the effective part of P. sylvestris provided by the invention is simple to operate, and the obtained extract is rich in lignin. The compound has a variety of pharmacological activities and can be used as an effective part of the plant.

Detailed ways

The substantial content of the present invention will be specifically described below with reference to the embodiments.

Example 1

The invention relates to a method for preparing an effective part of the white flower, which comprises the following steps: extracting the white flower of the succulent grass with a volume fraction of 50% ethanol for 3 times, each time for 2 hours, combining the extracts, and extracting the extract under reduced pressure to obtain an extract; After the extract is dissolved in an appropriate amount of water, it is first filtered with cotton and then separated by D101 type macroporous adsorption resin column chromatography, and sequentially eluted with pure water, 10%, 30%, 50%, 95% ethanol gradient, and collected 50%. The ethanol eluted fraction was separated by silica gel column chromatography, and eluted with chloroform-methanol in a volume ratio of 40:1 and 20:1, and the fraction eluted by 20:1 was collected and concentrated.

Among them, 1 kg of P. sylvestris was subjected to hot reflux extraction using 5 L of solvent, and 1 g of extract was dissolved in 5 mL of water.

Example 2

The invention relates to a method for preparing an effective part of the white flower, which comprises the following steps: extracting the white flower of the succulent grass with a volume fraction of 45% ethanol for 2 times, each time for 1 hour, combining the extracts, and extracting the extract under reduced pressure to obtain an extract; After the extract is dissolved in an appropriate amount of water, it is first filtered with cotton and then separated by D101 type macroporous adsorption resin column chromatography, and sequentially eluted with pure water, 10%, 30%, 50%, 95% ethanol gradient, and collected 50%. The ethanol eluted fraction was separated by silica gel column chromatography, and eluted with chloroform-methanol in a volume ratio of 40:1 and 20:1, and the fraction eluted by 20:1 was collected and concentrated.

Among them, 1 kg of succulent grass was subjected to hot reflux extraction using 4 L of solvent, and 1 g of extract was dissolved in 4 mL of water.

Example 3

The invention relates to a method for preparing an effective part of the white flower, which comprises the following steps: extracting the white flower of the succulent grass with a volume fraction of 55% ethanol for 4 times, each time for 3 hours, combining the extracts, and extracting the extract under reduced pressure to obtain an extract; After the extract is dissolved in an appropriate amount of water, it is first filtered with cotton and then separated by D101 type macroporous adsorption resin column chromatography, and sequentially eluted with pure water, 10%, 30%, 50%, 95% ethanol gradient, and collected 50%. The ethanol eluted fraction was separated by silica gel column chromatography, and eluted with chloroform-methanol in a volume ratio of 40:1 and 20:1, and the fraction eluted by 20:1 was collected and concentrated.

Among them, 1 kg of P. sylvestris was subjected to hot reflux extraction using 6 L of solvent, and 1 g of extract was dissolved in 6 mL of water.

The preparation method of the effective part of P. sinensis provided by the invention is simple to operate, and the obtained extract is rich in lignin compounds, has various pharmacological activities, and can be used as an effective part of P. sinensis for drug preparation.

Claims

The invention relates to a method for preparing an effective part of P. sylvestris, which comprises the following steps: refluxing white sage grass with a volume fraction of 45-55% ethanol for 2-4 times, each time 1-3 h, combining the extracts, The extract is concentrated under reduced pressure to obtain an extract; the extract is dissolved in an appropriate amount of water and then separated by macroporous adsorption resin column chromatography, and sequentially eluted with pure water, a 10%, 30%, 50%, 95% ethanol gradient. The 50% ethanol elution site was collected, and then separated by silica gel column chromatography, and eluted with chloroform-methanol in a volume ratio of 40:1, 20:1, and a 20:1 eluted fraction was collected and concentrated.
The preparation method according to claim 1, characterized in that the white flower is extracted by refluxing with a volume fraction of 50% ethanol.
The preparation method according to claim 1, wherein the hot reflux is extracted three times.
The preparation method according to claim 3, wherein each extraction is performed for 2 hours.
The preparation method according to claim 1, wherein 1 kg of succulent grass is subjected to hot reflux extraction using 4-6 L of a solvent.
The preparation method according to claim 1, wherein the extract is dissolved in an appropriate amount of water, filtered using cotton, and separated by macroporous adsorption resin column chromatography.
The preparation method according to claim 6, wherein 1 g of the extract is dissolved in 4 to 6 mL of water.
The preparation method according to claim 1, wherein the macroporous resin is of the D101 type.