WO2019050248A1

WO2019050248A1 - Centrifugal piston and centrifugal device comprising same

Info

Publication number: WO2019050248A1
Application number: PCT/KR2018/010273
Authority: WO
Inventors: 이준석
Original assignee: 이준석
Priority date: 2017-09-05
Filing date: 2018-09-04
Publication date: 2019-03-14

Abstract

A centrifugal piston according to an embodiment comprises: a piston body defining a path which extends from the front of the piston to the rear of the piston, and through which substances at the front of the piston can move to the rear of the piston; and a valve disposed on the path and configured to selectively open or block the path, wherein, during centrifugation in which centrifugal force acts on the piston, the substances at the front of the piston are centrifuged while the valve is blocking the path, and, when an external force is applied to the piston while the centrifugal force does not act on the piston, the valve moves freely relative to the piston body, and when the valve opens the path, at least part of the substances at the front of the piston can move to the rear of the piston.

Description

Centrifugal piston and centrifugal separator comprising same

Hereinafter, embodiments are directed to a centrifugal piston and a centrifugal separator including the same.

Since fat tissue obtained from a subject such as a human or an animal contains a large amount of oil and a mixture of impurities in a manner such as suction or incision, centrifugation of fatty tissue is performed to obtain pure fat. For example, Korean Patent Laid-Open No. 10-2015-0120797 discloses a piston. According to the structure of the piston disclosed herein, the two bodies are selectively rotated relative to each other to selectively open or close the flow path, thereby determining whether the material passes through the flow path.

The object of the present invention is to provide a centrifugal separator and a centrifugal separator including the centrifugal separator for blocking or opening a passage through which a substance passes depending on whether centrifugal force acts or not.

A centrifugal piston according to an embodiment includes a piston body extending from the front of the piston to the rear of the piston and defining a passage through which materials in front of the piston can move rearward of the piston; And a valve disposed on the passage and configured to selectively open or block the passage, wherein, during centrifugation in which the centrifugal force acts on the piston, the material in front of the piston, with the valve blocking the passage, When an external force is applied to the piston while the piston is not subjected to centrifugal force, the valve is relatively free to move relative to the piston body and at least part of the substances in front of the piston as the valve opens the passage Can be moved to the rear of the piston.

Wherein the center of rotation of the centrifugal force is located at the rear of the piston body and the piston body is located inside the container in which the piston is received and the piston body moves along the inside of the container in accordance with the external force, Lt; / RTI >

Wherein the piston body includes a receiving portion for receiving the valve and forming a portion of the passage, the receiving portion having an inner surface having a shape corresponding to at least a portion of the valve, the valve contacting the inner surface The passage may be blocked.

Said passage having a first path leading from one side of said piston body to a center of said piston body; And a second path extending from the center of the piston body to the rear of the piston body, the second path having a clearance formed between the valve and the inner surface when the valve is disengaged from the inner surface, . &Lt; / RTI >

A portion of the receiving portion having the inner surface may be narrowed toward the front of the piston along the longitudinal direction of the piston body.

A centrifugal separator according to one embodiment includes a piston body having a passage defined therein from the front of the piston to the rear of the piston, a valve disposed on the passage, and a valve disposed on the passage and between the piston body and the valve A piston including an installed sealing member; And a push rod for locking the valve to the piston body to block the passage and engaging the piston body and the valve, wherein when the push rod engages the piston body and the valve, It is possible to contact the sealing member and to block communication of the passage with the sealing member.

A portion of the push rod may be disposed within the piston body to fill a space between the piston body and the valve to form a seal of the passageway with the sealing member.

Wherein the sealing member prevents the valve from escaping from the piston body before the push rod engages the piston body and the valve, and after the push rod engages the piston body and the valve, The sealing member can be elastically deformed.

The piston may further include a groove in which a portion of the sealing member is embedded in the piston body.

The valve having a shape corresponding to an inner surface in front of the piston body; A valve back portion having a shape corresponding to a rear inner surface of the piston body; And a protruding element formed between the valve front portion and the valve rear portion and projecting toward an inner surface rearward of the piston body, wherein a distance between an inner surface at the rear of the piston body and the protruding element is smaller than a distance between the piston body The length of the portion of the sealing member facing the rear portion of the valve from the rear inner surface of the valve body.

A centrifugal separation method for separating biomaterials using a centrifugal piston according to an embodiment of the present invention is characterized in that the piston includes a piston body extending from the front of the piston to the rear of the piston and defining a passage through which biological materials can move, A valve disposed on the passage and configured to open or shut off the passage, wherein biomaterials are positioned in front of the piston body with respect to the container housing the piston and the biomaterials, and a centrifugal force is applied to the rear of the piston body The centrifugal separation method is characterized in that while the centrifugal force is applied to the piston, the biomaterials in front of the piston body are moved away from the front of the piston body with respect to the rotation center in a state where the valve blocks the passage Layer; A centrifugal force applied to the piston is released and a push rod is connected to the piston body to push the piston body in a direction toward the front of the piston body to apply pressure to the biomaterials, Wherein the biomaterial is removed from the vessel; The piston is pushed by the push rod in a state in which the push rod is disconnected from the piston body to apply pressure to the biomaterials in front of the piston and the valve opens the passage of the biomaterial, Moving biocompatible material closest to the body to the rear of the piston body through the passage; And obtaining the remaining at least one kind of biomaterial.

The centrifugal piston and the centrifugal separator including the centrifugal piston block the flow of the substances from the front of the piston to the rear of the piston during the centrifugal force, So that the desired material can be obtained.

The effects of the centrifugal piston and the centrifugal separator including the centrifugal separator according to one embodiment are not limited to those mentioned above, and other effects not mentioned can be clearly understood by those skilled in the art from the following description .

1 is a cross-sectional view schematically illustrating centrifugal separation of a centrifugal separator according to an embodiment of the present invention.

FIG. 2 is a cross-sectional view schematically illustrating removal of distal biomaterial from the front of the piston after centrifugal separation of the centrifugal separator according to an embodiment.

3 is a cross-sectional view schematically illustrating the removal of proximal biomaterial from the front of the piston after centrifugal separation of the centrifugal separator according to an embodiment.

4 is a perspective view schematically illustrating a piston according to an embodiment.

5 is an exploded side view schematically illustrating a piston according to one embodiment.

6 is a cross-sectional view schematically illustrating a passage of a piston according to an embodiment of the present invention.

7 is a cross-sectional view schematically showing a passage of a piston according to an embodiment of the present invention.

FIG. 8 is a cross-sectional view schematically showing a combined state of a piston and a push rod according to an embodiment.

Hereinafter, embodiments will be described in detail with reference to exemplary drawings. It should be noted that, in adding reference numerals to the constituent elements of the drawings, the same constituent elements are denoted by the same reference numerals even though they are shown in different drawings. In the following description of the embodiments, detailed description of known functions and configurations incorporated herein will be omitted when it may make the best of an understanding clear.

In describing the components of the embodiment, terms such as first, second, A, B, (a), and (b) may be used. These terms are intended to distinguish the constituent elements from other constituent elements, and the terms do not limit the nature, order or order of the constituent elements. When a component is described as being "connected", "coupled", or "connected" to another component, the component may be directly connected or connected to the other component, Quot; may be " connected, " " coupled, " or " connected. &Quot;

The components included in any one embodiment and the components including common functions will be described using the same names in other embodiments. Unless otherwise stated, the description of any one embodiment may be applied to other embodiments, and a detailed description thereof will be omitted in the overlapping scope.

As used herein, the term " subject " may be used to include organisms such as humans, animals, and the like.

The term " living tissue " as used herein means a tissue obtained from a subject. For example, the biological tissue may include adipose tissue. The biotissue can be obtained from a subject in a manner such as suction or incision.

As used herein, the term " piston front " refers to the upstream side relative to the flow stream of the biomaterial passing through the piston, and the term " rear piston " means downstream side relative to the flow stream of biomaterial passing through the piston do.

FIG. 1 is a cross-sectional view schematically illustrating centrifugal separation of a centrifugal separator according to an embodiment of the present invention. FIG. 2 is a cross-sectional view illustrating centrifugal separation of a distal biomaterial FIG. 3 is a cross-sectional view schematically illustrating the removal of proximal biomaterial from the front of the piston after centrifugal separation of the centrifugal separator according to an embodiment.

1 to 3, the centrifugal separator 1 according to the embodiment centrifugally separates biomaterials BL, FL and OL constituting a living tissue and then separates the biomaterials BL, ) Is removed by a predetermined method to obtain the desired biomaterial (FL). Hereinafter, the centrifugal separator 1 will exemplarily explain centrifugal separation of the fat tissue, which is one of the living tissues, but the embodiments are not necessarily limited thereto.

The centrifugal separator 1 may comprise a vessel 10, a piston 20 and a push rod 30.

The container 10 is configured to receive the fatty tissue, the piston 20, and the pushrod 30. The container 10 includes a substantially cylindrical main body 110. For example, the main body 110 may be a syringe. An outlet 120 is formed at the left end of the main body 110 on the front side of the main body 110 to allow the material forming fatty tissue to escape. An opening through which the fat tissue, the piston 20 and the push rod 30 are inserted is formed at the right end of the main body 110 with reference to the back of the main body 110 as shown in FIG.

The piston (20) is configured to move within the main body (110). The piston 20 may include a piston body 210, a valve 220, and at least one outer sealing member 230.

The piston body 210 is configured to move within the main body 110. The piston body 210 defines a passage from the front of the piston 20 to the rear of the piston 20. The piston body 210 may be substantially cylindrical.

The valve 220 is disposed on the passage defined in the piston body 210 to open or close the passage. For example, the valve 220 may selectively open or block the passage as centrifugal force acts. The valve 220 is received within the piston body 210 and is freely movable within the piston body 210.

The outer sealing member 230 is coupled to the outer side of the piston body 210 and configured to seal between the piston body 210 and the main body 110. The outer sealing member 230 is configured to contact the inner surface 112 of the main body 110. The piston body 210 can be fixed to the main body 110 at an arbitrary position of the main body 110 by the friction between the outer sealing member 230 and the inner surface 112 of the main body 110 .

The push rod 30 is configured to engage the piston body 210 and the valve 220. The push rod 30 can be pushed toward the front of the main body 110 (e.g., by an external force of the user) while the piston 20 is engaged with the piston body 210 and the valve 220 . At this time, some of the fatty tissue in front of the piston 20 can escape through the outlet 120. Further, the push rod 30 can be separated from the piston body 210 in a state of being engaged with the valve 220. At this time, the valve 220 can be separated from the piston body 210 together with the push rod 30.

The rotation axis X serving as the center of rotation of the centrifugal separator may be located on the rear side of the main body 110 and on the right side of the rear side of the piston body 210 with reference to FIG. In this case, in the main body 110, blood, liquid, pure fat, oil, or the like constituting adipose tissue is located on the left-hand side with reference to the front-side view 1 of the piston body 210, ).

Referring to FIG. 1, when the centrifugal separation is performed, the fat tissue located in front of the piston 20 is divided into the oil layer OL, the fat layer FL, , A blood and a liquid-receiving layer (BL). On the other hand, the piston body 210 and the valve 220 move in a direction away from the rotation axis X. The piston body 210 is fixed to the main body 110 by friction between the outer sealing member 230 coupled to the outer side of the piston body 210 and the inner side surface 112 of the main body 110. [ While the valve 220 freely moves within the piston body 210 and blocks the passage defined in the piston body 210. Since the valve 220 blocks the passage of the piston body 210 during the centrifugal force in which the centrifugal force is applied, the oil layer OL in front of the piston 20, the fat layer FL, The layer BL can not move through the passage. Some of the materials of the fat layer FL are very fine in size and can flow through the channels along with the oil of the oil layer OL in the course of performing centrifugation. Accordingly, in the process of performing centrifugation, the valve 220 cuts off the passage of the piston body 210, so that some substance of the fat layer FL, which is generally a user-desired substance, flows together with the oil of the oil layer OL, It is possible to prevent the rear cover 20 from moving from the front to the rear.

2, the push rod 30 may be coupled to the piston body 210 and the valve 220, respectively, in a state where centrifugal force is not applied to the piston 20 by centrifugal force. At this time, the state in which the valve 220 blocks the passage defined in the piston body 210 is maintained. When the outlet 120 of the main body 110 is opened and an external force is applied to the push rod 30 toward the front of the main body 110, the blood and the fluid layer BL located on the circle in front of the piston 20, The blood and the liquid of the blood are removed to the outside of the main body 110 through the outlet 120.

3, the push rod 30 can be separated from the piston body 210 and the valve 220 in a state where the centrifugal separation is completed and the blood and the fluid solution layer BL are removed from the container 10. Since the centrifugal force does not act on the piston 20, the valve 220 can freely move inside the piston body 210 and open the passage defined in the piston body 210. At this time, the oil of the oil layer OL located near the front of the piston 20 can flow from the front of the piston 20 to the rear of the piston 20 through the passage defined in the piston body 210 . The oil of the oil layer OL flowing backward of the piston 20 is removed from the container 10 and then the pure fat of the fat layer FL is obtained when the piston 20 is removed from the container 10 .

FIG. 4 is a perspective view schematically illustrating a piston according to an embodiment, and FIG. 5 is an exploded side view schematically showing a piston according to an embodiment.

FIG. 6 is a cross-sectional view schematically showing a passage of a piston according to an embodiment, and FIG. 7 is a cross-sectional view schematically showing a passage of a piston according to an embodiment.

4 to 8, a piston 20 according to one embodiment may include a piston body 210, a valve 220, at least one outer sealing member 230, and an inner sealing member 240 have.

Outside the piston body 210, an inlet 2112 configured to introduce a substance may be formed. For example, after the oil of the oil layer OL described with reference to FIG. 3 has entered the interior of the piston body 210 through the inlet 2112, it can flow through the passage defined in the piston body 210 have. At least one recess 219 configured to engage at least one outer sealing member 230 may be formed on the outer side of the piston body 210.

The passage defined in the piston body 210 may have at least one directionality. For example, the passage may include a first path 2114 leading from the one side of the piston body 210 to the center of the piston body 210 and a second path 2114 extending from the center of the piston body 210 to the rear of the piston body 210 2 path 2116 and a third path 2118 leading from the rear of the piston body 210 to the exterior of the piston body 210. The first path 2114 is configured to be in fluid communication with the inlet 2112 and the second path 2116 is configured to selectively fluidly communicate with the first path 2114 and the third path 2118, (2118) is configured to be in fluid communication with the exterior of the piston body (210).

In one embodiment, the passageway defined in the piston body 210 may further include a chamber 2115 located between the first pass 2114 and the second pass 2116. The chamber 2115 may be located at the center of the piston body 210. When fluid communication between the first path 2114 and the third path 2118 through the second path 2116 is blocked, material may remain within the chamber 2115. Fluid communication between the chamber 2115 and the second path 2116 may be blocked while the valve 220 contacts the first inner surface 2122 of the receiving portion 212. [

The piston body 210 may include a receiving portion 212 configured to receive the valve 220. The receptacle 212 may have internal surfaces having a shape corresponding to the external shape of the valve 220. For example, the receptacle 212 may have a first inner surface 2122 and a first inner surface 2122 that are tapered along the front and rear of the piston body 210, A second inner surface 2124 may be provided. In one example, the first inner surface 2122 may be configured to gradually increase in width from the front of the piston 20 toward the rear of the piston 20.

The piston body 210 may include a piston engagement portion 214 adapted to engage the push rod 30. [ For example, the piston engagement portion 214 may include threads formed in the second inner surface 2124 of the receiving portion 212.

The valve 220 may include a valve front portion 222 and a valve back portion 224.

The valve front portion 222 may be configured to block the passage. The valve front portion 222 may contact the inner surface of at least a portion of the receiving portion 212. For example, the valve front portion 222 may contact the first inner surface 2122 of the receiving portion 212. The valve front portion 222 may have a shape corresponding to the first inner surface 2122 of the receiving portion 212.

A clearance may be formed between the outer surface 2222 of the valve front portion 222 and the first inner surface 2122 of the receiving portion 212. This gap may be formed when the valve front portion 222 is separated from the first inner surface 2122. 6, when the valve front portion 222 contacts the first inner surface 2122 of the receiving portion 212, the outer surface 2222 of the valve front portion 222 and the receiving portion 212, There is no clearance between the first inner surface 2122 of the receptacle 212 and the valve front 222 as shown in Figure 7. When the valve front 222 is separated from the first inner surface 2122 of the receptacle 212, A gap is formed between the outer surface 2222 of the front portion 222 and the first inner surface 2122 of the receiving portion 212. [ This gap may form at least a portion of the second path 2116.

The valve back 224 is configured to guide the movement of material along the passageway. The valve back portion 224 may be parallel to the inner surface of at least a portion of the receiving portion 212. For example, the valve back portion 224 may be parallel to the second inner surface 2124 of the receiving portion 212. The valve back portion 224 extends from the valve front portion 222 toward the rear of the piston body 210.

In one embodiment, the valve 220 may include a protruding element 226. The protruding element 226 may be formed between the valve front portion 222 and the valve back portion 224. The protruding elements 226 may protrude toward the inner surfaces of the piston body 210. In one example, protruding element 226 may protrude toward second inner surface 2124 of receptacle 212.

In one embodiment, the valve 220 may include a valve engagement portion 2242. The valve engagement portion 2242 is configured to engage the push rod 30. For example, the valve engagement portion 2242 may have a male engagement formation. The shape of the valve engagement portion 2242 can be appropriately set in accordance with the shape of the push rod 30. The valve engagement portion 2242 can extend from the valve back portion 224 toward the rear of the piston body 210. [

The inner sealing member 240 is configured to prevent the valve 220 from separating from the piston body 210. The inner sealing member 240 can be installed between the inner surfaces of the piston body 210 and the valve 220 on the passage defined by the piston body 210. [ For example, the inner sealing member 240 may be installed on the second inner surface 2124 of the receiving portion 212 on the third path 2128.

In one embodiment, the inner sealing member 240 is configured to engage the protruding element 226. The inner sealing member 240 may have an annular shape. The distance D1 between the second inner surface 2124 of the receiving portion 212 and the protruding element 226 is greater than the distance D1 between the second inner surface 2124 of the receiving portion 212 and the inner sealing member 240. [ May be smaller than the distance D2 to the inner side of the base end portion.

In one embodiment, a portion of the inner sealing member 240 may be embedded in the piston body 210. For example, the receiving portion 212 may include a groove 2126 configured to receive a portion of the inner sealing member 240. Grooves 2126 may be formed in the second inner surface 2124.

According to this structure, when the valve 220 attempts to escape to the outside of the piston body 210 before the push rod 30 is engaged with the piston body 210 and the valve 220, And is not separated from the piston body 210 by the inner sealing member 240.

The push rod 30 is configured to engage the piston body 210 and the valve 220 and to secure the valve 220 to the piston body 210 to block the passage defined in the piston body 210. The push rod 30 includes a first push rod engagement portion 342 configured to engage the piston engagement portion 214 and a second push rod engagement portion 342 located inside the push rod engagement portion 342 and opposite the valve backward portion 224 And a second push rod coupling portion 344 surrounding the valve coupling portion 2242 and engaging with the valve coupling portion 2242. [ For example, the first push rod coupling portion 342 may have a threaded shape.

When the push rod 30 is engaged with the piston body 210 and the valve 220, a part of the push rod 30 contacts the inner sealing member 240 and can block the passage. For example, the push rod 30 may include a seal forming portion 346. The seal forming portion 346 fills at least a portion of the space between the piston body 210 and the valve 220 and can contact the inner sealing member 240. Thus, the seal forming portion 346 can block communication of the passage together with the first push rod coupling portion 342 and the second push rod coupling portion 344. The seal forming portion 346 may form a seal of the third path 2118 between the second inner surface 2124 of the receptacle 212 and the valve back portion 224.

The contact between the seal forming portion 346 and the inner sealing member 240 is such that after the push rod 30 is engaged with the piston body 210 and the valve 220, the push rod 30, together with the valve 220, (210). &Lt; / RTI > While the seal forming portion 346 contacts the inner seal member 240, the inner seal member 240 can elastically deform or maintain its deformation.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. For example, it is to be understood that the techniques described may be performed in a different order than the described methods, and / or that components of the described systems, structures, devices, circuits, Lt; / RTI > or equivalents, even if it is replaced or replaced.

Claims

In a centrifugal piston,

A piston body extending from the front of the piston to the rear of the piston and defining a passage through which materials in front of the piston can move rearward of the piston; And

A valve disposed on the passage and configured to selectively open or block the passage;

Lt; / RTI >

When centrifugal force is applied to the piston, the substances in front of the piston are centrifuged in a state where the valve blocks the passage, and when an external force is applied to the piston while the centrifugal force is not applied to the piston, Wherein at least a portion of the material in front of the piston is capable of moving rearwardly of the piston as the valve moves relative to the body and the valve opens the passage.
The method according to claim 1,

The center of rotation of the centrifugal force is located behind the piston body,

Wherein the piston body is located inside a vessel in which the piston is received,

Wherein the piston body moves along the interior of the vessel according to an external force and exerts a pressure on the material in front of the piston.
The method according to claim 1,

The piston body including a receiving portion for receiving the valve and defining a portion of the passage,

The receiving portion having an inner surface having a shape corresponding to at least a portion of the valve and the passage being blocked when the valve contacts the inner surface.
The method of claim 3,

The passage

A first path extending from one side of the piston body to the center of the piston body; And

A second path extending from the center of the piston body to the rear of the piston body;

Lt; / RTI >

Wherein the second path includes a gap formed between the valve and the inner surface when the valve is disengaged from the inner surface.
The method of claim 3,

Wherein a portion of the receiving portion having the inner surface is narrowed toward the front of the piston along the longitudinal direction of the piston body.
1. A piston comprising: a piston body in which a passage leading from the front of the piston to the rear of the piston is defined; a valve disposed on the passage; and a sealing member disposed on the passage and between the piston body and the valve; And

A push rod for locking said valve to said piston body to block said passage and engaging said piston body and said valve;

Lt; / RTI >

Wherein when the push rod engages the piston body and the valve, the push rod contacts the seal member and blocks communication of the passage with the seal member.
The method according to claim 6,

Wherein a portion of the push rod is disposed within the piston body to fill a space between the piston body and the valve to form a seal of the passageway with the sealing member.
The method according to claim 6,

Before the push rod engages the piston body and the valve, the sealing member prevents the valve from departing from the piston body,

Wherein the sealing member is resiliently deformed before the push rod is engaged with the piston body and the valve and then separated from the piston body together with the valve.
The method according to claim 6,

Wherein the piston further comprises a groove in which a portion of the sealing member is embedded in the piston body.
A centrifugal separation method for separating biomaterials using a centrifugal separation piston,

The piston comprising a piston body extending from the front of the piston to the rear of the piston and defining a passage through which the biomaterials are movable, and a valve disposed on the passage and configured to open or close the passage,

Biomaterials are positioned in front of the piston body and a rotational center of a centrifugal force is located in the rear of the piston body,

The centrifugation method

Separating the biomaterials in front of the piston body in a direction away from the front of the piston body with respect to the center of rotation while the valve is blocking the passage while centrifugal force is applied to the piston;

A centrifugal force applied to the piston is released and a push rod is connected to the piston body to push the piston body in a direction toward the front of the piston body to apply pressure to the biomaterials, Wherein the biomaterial is removed from the vessel;

The piston is pushed by the push rod in a state in which the push rod is disconnected from the piston body to apply pressure to the biomaterials in front of the piston and the valve opens the passage of the biomaterial, Moving biocompatible material closest to the body to the rear of the piston body through the passage; And

Obtaining at least one kind of remaining biomaterial remaining;

&Lt; / RTI >