WO2019041256A1

WO2019041256A1 - Head-mounted ultrasonic transmission apparatus

Info

Publication number: WO2019041256A1
Application number: PCT/CN2017/099996
Authority: WO
Inventors: 郑海荣; 邱维宝; 黎国锋; 苏敏; 蔡蕊琳; 李永川
Original assignee: 深圳先进技术研究院
Priority date: 2017-08-31
Filing date: 2017-08-31
Publication date: 2019-03-07

Abstract

Provided is a head-mounted ultrasonic transmission apparatus, wherein same falls within the technical field of ultrasonic neural stimulation research, and comprises a base (2) capable of being arranged on a laboratory animal skull (9) and a top cover (1) connected to the base (2), wherein an inner cavity for accommodating a coupling agent is provided in the base (2), and the top cover (1) is connected to an ultrasonic transducer with a working face facing a bottom portion of the inner cavity. The head-mounted ultrasonic transmission apparatus further comprises a fixing device for fixing the base (2) to the laboratory animal skull (9), wherein the fixing device comprises a skull connector (31), with a front end thereof being capable of being embedded into the laboratory animal skull (9), and the base (2) is attached to the laboratory animal skull (9) and is directly connected to the fixing device or is connected to the fixing device via a binder (32). The head-mounted ultrasonic transmission apparatus can implement positionally accurate and controllable and reproducible ultrasonic stimulation on the brain of a free-moving laboratory animal, and is suitable for carrying out animal experiments related to feelings, emotions, cognition and memory.

Description

Head-mounted ultrasonic conduction device

Technical field

[0001] The present invention belongs to the technical field of ultrasonic nerve stimulation research, and in particular, to a head-mounted ultrasonic conduction device.

Background technique

[0002] Neurostimulation and neuromodulation refers to the use of electrical, electrical, acoustic, thermal, thermal or mechanical stimuli and chemical stimuli, acting on the living nervous system (such as organisms, organs, tissues, cells, etc.) and causing reactions the process of. The effects of stimulation regulation can be divided into excitatory stimuli that cause function display or enhancement and inhibitory stimuli that cause function to disappear or weaken. Stimulation and regulation of neural activity contribute to the study of neurological diseases and mental illnesses. Peer is also the basic means of studying human brain perception, movement and cognitive function.

[0003] Ultrasound stimulation is an ancient and emerging non-invasive nerve stimulation. As early as 1929, there have been reports of ultrasound on the effective regulation of the nerve (heart) muscles of frogs and turtles. Subsequently, in 1958, literature records that the ultrasound penetrated the skull of the scorpion cat and reversibly inhibited the photoexcitation potential of the retina. High intensity focused ultrasound (HIFU) is often used as an intervention in the central nervous system. In 2008, Tyler et al. confirmed that ultrasound stimulated hippocampal slices, which stimulated action potentials, pressure-controlled sodium ions, calcium ion transients, and synaptic transmission. In 2010, the Tyler team first performed positive transcranial ultrasound to stimulate the cranial motor cortex of mice, which induced action potentials and caused action responses of limbs and tails. Since then, the use of ultrasound as a means of nerve stimulation and regulation has attracted widespread attention.

[0004] However, the mechanism of action and application parameters of ultrasound stimulation are not sufficiently clear. In addition to the development of cell-based and ex vivo tissue-based studies, conscious, freely active rodent small animals (especially mice with a rich disease model and widely used in biomedical applications) are associated with memory learning cognition. Behavioral experiments are also very important. However, current ultrasound stimulation for animals is typically achieved using a bulky single-element focused ultrasound transducer. Because of the large size and heavy weight of the transducer, the probe must be fixed by the corresponding positioning device during use, while the stimulated animal needs to be anesthetized and its head needs to be fixed. These steps have greatly limited the development of behavioral experiments. Designing an ultrasound stimulating device for awake, free-living small animals will help drive the application of ultrasound stimulation technology in neuroscience. [0005] In the prior art, the patent document "Wearable Transcranial Ultrasound Neural Stimulation and Electrophysiological Recording System and Method" of the application No. 201610326489.0, a wearable transcranial ultrasound nerve stimulation and electrophysiological recording is proposed. The joint system includes: a computer control and data processing unit, a digital to analog/analog to digital conversion unit, a power amplifier, a wearable ultrasonic transducer, an electrode assembly, and a signal amplification unit. The "wearable ultrasonic transducer" mentioned in the above only mentions that the transducer and the subject are fixed by a sealing tape, and the mutual contact faces are to be coupled. Obviously, for conscious animals, their activities are frequent and the scalp is extremely easy to slide relative to the skull. It is difficult to accurately and reliably fix the ultrasound probe to perform ultrasonic stimulation using only a sealing tape. Further, the electrode assembly mentioned in the patent realizes the mounting of the recording electrode by providing a through hole in the ultrasonic transducer, and the above method of fixing the transducer by the sealing tape is difficult to ensure proper mounting of the recording electrode.

[0006] The application No. 201610327257.7, "a wirelessly controlled wearable ultrasonic nerve stimulation device", proposes a wireless wearable ultrasonic nerve stimulation device, comprising: a user control terminal, Control the transmitter chip, ultrasonic transducer and patch housing. The "patch housing" mentioned herein only refers to the filling couplant between the patch housing and the object and is fixed by a sealing tape. Obviously, for awake animals, their activities are frequent and the scalp is very easy to slide relative to the skull. It is difficult to accurately and reliably fix the ultrasound probe with a sealing tape alone; there is no mention of an effective structure to prevent coupling between the ultrasonic transducer and the object. The agent slipped and lost.

[0007] The patent document "Awakening Animal Ultrasound Neuroregulator" of the application No. 201710138442.6 proposes a conscious animal neuromodulation device, including: a pulse signal generating module, an ultrasonic transducer module and a fixed module. The "fixed modules" mentioned herein only describe in general terms the immobilization of immobilization modules to animal neuromodulation targets by industry-wide methods. However, how to design a reasonable structure to achieve a reliable connection between the ultrasonic transducer and the skull and ensure that the ultrasound can be effectively transmitted to the inside of the skull is a critical technology for the entire ultrasound stimulation system. The above patent does not propose a feasible solution. .

[0008] In the prior art, the ultrasonic nerve stimulation technique uses a large-volume, heavy-weight ultrasonic probe to achieve ultrasonic stimulation, and the stimulated animal needs to be anesthetized, and the head needs to be fixed, so the above-mentioned ultrasonic stimulation is not Suitable for animal experiments related to feeling, emotion, cognition and memory. Moreover, the existing wearable ultrasound stimulation program (application number is 201610326489 .0, 201610327257

.7, 201710138442.6 (documents), does not clearly suggest how to securely fix the ultrasonic transducer to the skull or simply mention the fixing method using a sealing tape. [0009] In summary, in the prior art, for a small animal stimulating device in the field of ultrasonic nerve stimulation technology, it is difficult to apply an accurate and controllable and reproducible stimulus to a awake, freely movable small motion, and the implementation effect is insufficient. Good question.

technical problem

[0010] The object of the present invention is to overcome the above deficiencies of the prior art and to provide a head-mounted ultrasonic transmission device.

It can perform ultrasonic nerve stimulation research on experimental animals in the case of awake, freely movable animals, etc., and implements accurate and controllable and reproducible ultrasonic stimulation on the brain of freely moving experimental animals, which is suitable for use in marginal development. Animal experiments related to feelings, emotions, cognition, etc. and memory are effective.

Problem solution

Technical solution

[0011] The technical solution of the present invention is: a head-mounted ultrasonic transmission device, comprising: a base that can be placed on a skull of an experimental animal; and a top cover connected to the base, the base has a An inner cavity accommodating a coupling agent, the top cover being coupled with an ultrasonic transducer having a working surface facing the bottom of the inner cavity; the head-mounted ultrasonic conduction device further comprising a base for fixing the base to the skull of the experimental animal The fixing device comprises a skull connector which can be embedded in the skull of the experimental animal, and the base is attached to the skull of the experimental animal and directly connected to the fixing device or connected by an adhesive.

[0012] Optionally, a side of the base near the bottom is provided with a convex structure; or / and, a side of the base near the bottom is provided with a groove structure.

[0013] Optionally, the skull connector is a threaded connection, the adhesive is dental cement;

[0014] the convex structure is an everted convex edge, and the adhesive is connected to the skull connecting piece and the everted convex edge

[0015] Alternatively, the groove structure is a groove disposed on an outer side surface of the base, and the adhesive is coupled to the skull connecting member and embedded in the groove.

[0016] Optionally, the base is disposed on a side of the front side of the skull of the experimental animal with a first protrusion, or/and the base is disposed adjacent to a side of the back side of the skull of the experimental animal. There is a second projection.

[0017] Optionally, the bottom of the base is configured as a curved structure, or/and the bottom of the base is provided with a resilient washer. [0018] Optionally, the base has an inverted cone shape that is large and small, or/and the top cover is fixedly or detachably connected to the top of the base.

[0019] Optionally, the top cover is connected to the base by a card structure, a snap structure or a threaded structure.

[0020] Optionally, the base is provided with at least one L-shaped locking groove, the upper end of the L-shaped locking groove is opened, and the upper end opening of the L-shaped locking groove is located at an upper end surface of the base, the top The cover is provided with a lock block that can be caught in the L-shaped lock groove.

[0021] Optionally, a inner side of the top cover is provided with a convex structure for limiting the ultrasonic transducer; or / and, the inner side of the top cover is provided with a wire groove for accommodating the cable.

[0022] Optionally, a side of the base is provided with a conduit structure for achieving at least one of ultrasonic stimulation and electrophysiological collection, electrical stimulation, administration, microdialysis, light stimulation, and light imaging, and the catheter structure includes At least one conduit in which at least one of an electrode, a thin tube or an optical fiber is disposed.

Advantageous effects of the invention

Beneficial effect

[0023] A head-mounted ultrasonic conduction device provided by an embodiment of the present invention provides an accurate and controllable and reproducible ultrasonic stimulation of a brain of a freely moving experimental animal, and allows The function of intracranial administration, microdialysis, electrical stimulation, light stimulation or electrophysiological acquisition in ultrasound-stimulated peers.

Brief description of the drawing

DRAWINGS

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings to be used in the embodiments will be briefly described below. Obviously, the drawings in the following description are only some implementations of the present invention. For example, other drawings may be obtained from those of ordinary skill in the art in light of the inventive work.

1 is a front view of a head-mounted ultrasonic conduction device connected to a skull of an experimental animal according to an embodiment of the present invention;

2 is a right side view of a head-mounted ultrasonic conduction device connected to a skull of an experimental animal according to an embodiment of the present invention;

3 is a main body of a head-mounted ultrasonic conduction device connected to a skull of an experimental animal according to an embodiment of the present invention; View.

Embodiments of the invention

The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It is understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0029] It is to be noted that when an element is referred to as being "fixed" or "in" another element, it can be directly on the other element or possibly the same. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or the same element.

[0030] It should also be noted that the left, right, upper, lower, and the like orientations in the embodiments of the present invention are merely relative concepts or referenced to the normal use state of the product, and should not be considered as having Restrictive.

[0031] As shown in FIG. 1 and FIG. 2, a head-mounted ultrasonic transmission device according to an embodiment of the present invention includes a base 2 that can be placed on a skull 9 of an experimental animal, and a top cover 1 connected to the base 2, the base. 2 has a cavity for accommodating the couplant, the upper and lower ends of the base 2 are smashed, the lower end is located on the skull 9 of the experimental animal, and the upper end is used to connect the top cover 1; the top cover 1 is connected with the working surface facing An ultrasonic transducer (not shown) of the bottom of the lumen (ie, the skull of the experimental animal), the head-mounted ultrasonic transducer further includes a fixing device for fixing the base 2 to the skull 9 of the experimental animal, the fixing device including the front end The skull connector 31 can be embedded in the skull of the experimental animal. The front end of the skull connector 31 can be embedded and connected to the skull of the experimental animal. The skull connector 31 is not easy to loose, and the connection reliability is good. The bottom of the base 2 is attached to the skull of the experimental animal. And the base 2 is directly connected to the skull connector 31 or connected by an adhesive 32. That is, the base 2 can be directly fixed by the skull connecting member 31, or the base 2 can also be fixed by connecting the adhesive 32 to the skull connecting member 31. The skull connecting member 31 can be a threaded connecting member (for example, a skull screw), and the front end thereof can be screwed to the skull of the experimental animal without damaging the brain tissue of the experimental animal, and the base 2 can be stably and reliably attached to the skull of the experimental animal. Therefore, it is possible to perform ultrasonic nerve stimulation research on experimental animals in the case of conscious and freely movable animals, and to perform accurate and controllable and reproducible ultrasonic stimulation on the brain of freely moving experimental animals, which is suitable for use in the exhibition. The animal experiment related to feeling, emotion, cognition, etc. and memory, and the head-mounted ultrasonic conduction device provided by the embodiment is small in size and light in weight, and can be applied not only to small and medium-sized animals such as rabbits. It can also be applied to the study of small animals such as mice. [0032] Optionally, a convex structure for connecting the adhesive 32 or the skull connecting member 31 is disposed on a side of the base 2 near the bottom; or/and, as shown in FIG. 3, the base A groove structure for joining the adhesive 32 or the skull connector 31 is provided on the side near the bottom.

[0033] Alternatively, the raised structure may be an everted flange 23 to increase the contact surface with the adhesive 32, and the connection reliability is high. Of course, it is also possible to press the skull connector 31 directly against the everted flange 23, or to provide a hole for the skull connector 31 to pass through at the everted flange 23. In a particular application, the skull connector 31 is provided with one or at least two and is located around the everted flange 23, and the adhesive 32 is attached to the skull connector 31 and the everted flange 23. The skull connector 31 is provided with a plurality of jaws, and the adhesive 32 and the skull connector 31 can be disposed on the left and right sides or/and the front and rear sides. The number and arrangement of the adhesive 32 and the skull connector 31 can also be set according to the actual situation. The valgus protrusion 23 may be in the shape of a ring, or may be in the form of a column, a block or a sheet.

[0034] Optionally, as shown in FIG. 3, the groove structure is a groove 20 disposed on an outer side of the base 2, and the adhesive 32 is coupled to the skull connecting member 31 and embedded in the Included in the recess 20. The groove 20 may be an annular groove or a separate groove (for example, a circular groove or a rectangular groove) circumferentially disposed along the base 2, and a portion of the adhesive 32 (for example, dental cement) is embedded in the groove 20 to form an inverted structure. , the connection is highly stable.

[0035] Alternatively, the adhesive 32 may be an adhesive having sufficient mechanical strength after solidification, such as dental cement or other type of adhesive 32.

[0036] Optionally, the base 2 is provided with a first protrusion 21 on a side close to the front side of the skull 9 of the experimental animal, or / and, the base 2 is provided with a second side on the side of the back side of the skull 9 of the experimental animal. The number and specific structure of the projections 22, the projections (the first projections 21 and the second projections 22) can be set according to actual conditions for increasing the fixing strength of the dental cement to the base 2.

[0037] Optionally, the bottom of the base 2 (the everted flange 23) is configured as a curved structure that can match the shape of the skull of the skull 9 of the experimental animal. For example, the bottom of the base 2 may have a suitable shape such as a saddle shape.

[0038] Optionally, the bottom of the base 2 may be provided with a resilient washer (not shown), the elastic washer may be a silicone gasket, etc., and has good sealing performance and a certain buffering effect.

[0039] Optionally, the base 2 has an inverted cone shape that is large and small, and the lower end of the base 2 has a relatively small size to match the size of the head of the laboratory animal. The upper end of the base 2 has a large size, and a large diameter ultrasonic transducer can be installed. The low-frequency, high-intensity ultrasonic waves can be used to stimulate the experimental animals.

[0040] Optionally, the piezoelectric material of the ultrasonic transducer in the top cover 1 may adopt a piezoelectric composite material, a piezoelectric ceramic, Piezoelectric single crystal materials and other piezoelectric materials, the thickness of the piezoelectric material can be in the range of 0.01-3 mm; the ultrasonic transducer can be a single-element ultrasound transducer or a multi-element ultrasound transducer, or a focused ultrasound Transducer or planar ultrasound transducer. The ultrasonic transducer in the top cover 1 may be air backed or may be filled with other materials as a backing.

[0041] Optionally, the top cover 1 is fixedly or detachably connected to the top of the base 2. In a specific application, the top cover 1 can be detachably connected to the base 2 by a screw card structure, a snap structure or a thread structure, and the top cover 1 can be repeatedly and conveniently removed from the top cover 1 . Of course, as an alternative, the top cover 1 can also be fixedly connected to the base 2, or the top cover 1 can be connected to the base 2 body.

[0042] Alternatively, in order to facilitate the disassembly and assembly of the top cover 1, an anti-slip structure may be disposed outside the top cover 1. The anti-slip structure can be a projection 11 or / and a groove.

In this embodiment, the base 2 is provided with at least two L-shaped lock grooves 24, and the L-shaped lock grooves 24 penetrate through the side walls of the base 2.

The upper end of the L-shaped lock groove 24 is located at the upper end of the base 2, and the top cover 1 is provided with a lock block 14 that can be caught in the L-shaped lock groove 24. The number of the lock block 14 can be It is equal to the number of L-shaped lock grooves 24. The lock block 1 4 may be in the shape of a column or a mushroom. The lock block 14 can be longitudinally engaged from the upper end of the L-shaped lock groove 24, and then the top cover 1 is rotated to make the lock block 14 laterally snap into the L-shaped lock groove 24, which is convenient and reliable. After installing the top cover 1 and the ultrasonic transducer 吋, the ultrasonic coupling agent pre-filled into the base 2 can automatically overflow from the L-shaped lock groove 24 after filling the inner cavity space of the base 2, ensuring that the ultrasonic coupling agent is filled with the ultrasonic transducer and Experimental animal between skulls 9. The L-shaped lock groove 2 4 can also adopt the lock groove of other structural shapes, and the number of the L-shaped lock grooves 24 can be set according to actual conditions.

[0044] Optionally, an overflow hole may be disposed near the upper end of the base 2, and if the top cover 1 and the base 2 are connected by a thread structure, the top cover 1 and the ultrasonic transducer 安装 are installed, and the base 2 is pre-filled. The ultrasonic coupling agent can flow out of the overflow hole after filling the inner cavity space of the base 2.

[0045] Alternatively, the top of the top cover 1 may be provided with a wire through hole and a screw hole to facilitate installation of an ultrasonic transducer or the like.

[0046] Optionally, a convex structure (not shown) for limiting the ultrasonic transducer is disposed inside the top cover 1 to facilitate installation and positioning of the ultrasonic transducer. In a specific application, the protruding structure may be a convex ring or a protruding rod integrally formed on the inner side of the top cover 1 or fixedly connected to the inner side of the top cover 1.

[0047] Optionally, the inner side of the top cover 1 is provided with a wire groove (not shown) for accommodating the cable, and the cable is connected to the ultrasonic transducer, and the diameter of the ultrasonic transducer can be the same as that of the top cover 1. Inner diameter matching, ultrasonic transducer will not loose It is good for stable ultrasonic stimulation of experimental animals.

[0048] Optionally, the side of the base 2 is provided for achieving ultrasonic stimulation and electrophysiological collection, electrical stimulation, administration

a catheter structure 4 of at least one of (non-therapeutic purpose), microdialysis, light stimulation, and photo imaging, the catheter structure 4 includes at least one catheter, and at least one of an electrode, a thin tube, or an optical fiber is disposed in the catheter, thereby The function of intracranial administration, microdialysis, electrical stimulation, light stimulation or electrophysiological acquisition of ultrasound-stimulated common targets.

[0049] FIG. 1 shows a schematic view of a head mounted ultrasound transducer mounted on a skull 9 of an experimental animal. The bottommost dotted ellipse represents the sagittal plane of the small animal skull 9, the skull is tightly coupled to the upper head-mounted ultrasound transducer and is secured by the skull connector 31 and dental cement; the upper head-mounted ultrasound transducer includes a cap 1 and the base 2 are two parts; the base 2 and the top cover 1 can be processed by any material such as metal, plastic, plexiglass or the like which is solid at normal temperature after processing. The top cover 1 and the base 2 can be connected by an L-shaped lock groove 24; a circular ultrasonic transducer can be installed inside the top cover 1, and the ultrasonic transducer can include an ultrasonic piezoelectric material, a matching layer and a lead wire (not shown) Out, for generating ultrasonic waves to radiate downward; the top of the top cover 1 has three lock blocks 14 for interlocking connection with the L-shaped lock grooves 24 of the base 2; the top of the top cover 1 has two symmetrical anti-slip protrusions Structure (protrusion 11); The base 2 is in the shape of an inverted hollow cone, including an upper jaw and a lower jaw; the inverted hollow cone shape can reduce the area occupied by the base 2 and allow the ultrasonic transducer to have a higher Large radiation area, thus ensuring that the device can be applied to small animals and has high ultrasonic radiation intensity and low radiation frequency; there are three L-shaped lock grooves 24 around the outlet of the base 2, which can be external to the top cover 1 The three lock blocks 14 are interlocked; the outer edge of the bottom of the base 2 has an everted outer edge structure, and the bottom surface of the outer edge structure is not a flat surface, but a cylindrical surface or a curved surface, and the outer curved structure of the cylindrical surface is small animal The surface of the skull arched by the curved surface Anastomosis, thereby achieving a seamless close installation of the base 2 and the skull; the outer and outer edges of the outer everted structure respectively have a convex structure, and the front and rear convex structures can be added without increasing the width of the outer edge of the base 2 Under the premise, the contact area of the dental cement is increased to prevent the base 2 from rotating around the central axis, thereby improving the mechanical strength of the mounting base 2. Figure 2 is a side view of the head-mounted ultrasonic transducer, labeled similar to that shown in Figure 1, in particular, in the right side of the base 2 in Figure 2 (i.e., behind the skull of the experimental animal), the catheter structure 4 is designed. Through the catheter structure 4, an electrophysiological collection or stimulation electrode, a drug delivery tube, a dialysis tube, an optical fiber and the like may be additionally installed, and the lower end of the tube may extend to different depths of the ultrasound target area to realize electrophysiological signal collection, Site-specific drug delivery, microdialysis, light stimulation and other functions. [0050] A head-mounted ultrasonic conduction device provided by an embodiment of the present invention is mainly used for performing repetitive operation of ultrasonic stimulation on a awake and freely moving small animal, mainly comprising a top cover 1 and a base 2, and a top cover 1 built-in ultrasonic transducer, the ultrasonic transducer comprises a piezoelectric material and a matching layer, which can generate ultrasonic to achieve ultrasonic stimulation; the base 2 can be in the shape of an inverted hollow cone with upper and lower jaws; An L-shaped lock groove 24 is detachably connected to the top cover 1; the lower jaw has an outer curved outer edge structure having a bottom surface and two outer edge protruding structures on the outer side of the outer edge structure (first convex The upper portion 21 and the second protruding portion 22); the upper and lower jaws are respectively used for connecting the top cover 1 and the experimental animal skull 9; and the top cover 1 can be repeatedly installed and disassembled. A head-mounted ultrasonic transmission device provided by an embodiment of the present invention has at least the following advantages:

[0051] (1) The bottom surface of the lower exit of the base 2 is curved, and the valgus flange 23 and the protruding portion may also have a curved shape, so that the base 2 can be in close contact with the curved skull of the experimental animal, thereby improving the contact. The accuracy of the mounting position of the base 2 and the service life of the device;

[0052] (2) The base 2 has an inverted hollow cone shape, which not only reduces the area occupied by the base 2, but also allows the ultrasonic transducer to have a larger radiation area, thereby ensuring that the device can be applied to small animals and has Higher ultrasonic radiation intensity and lower radiation frequency;

[0053] (3) The bayonet structure of the base 2 and the top cover 1 ensures that the top cover 1 can be repeatedly installed and disassembled, thereby facilitating the flexible experiment of repeated ultrasonic stimulation;

[0054] (4) The bayonet structure of the base 2 and the top cover 1 allows the ultrasonic coupling agent pre-filled into the top cover 1 to automatically overflow after filling the inner cavity space of the base 2, ensuring that ultrasonic energy can pass through the base 2 The cavity is reliably conducted without increasing the pressure in the cavity of the base 2;

[0055] (5) The convex structure and the wire groove structure on the inner side of the top cover 1 enable the ultrasonic sensor (ultrasonic transducer) to operate in the air backing mode to improve the efficiency of ultrasonic radiation;

[0056] (6) The outer side wall of the base 2 can be installed with a radial catheter, allowing the passage of a drug guiding tube, an electrophysiological electrode or an optical fiber, for performing ultrasound stimulation combined with intracranial administration, microdialysis, electrophysiological collection, electrical stimulation or Experiments such as light stimulation;

[0057] (7) The entire device is small in size and light in weight, and can be applied to small animals such as mice that are free to move, and perform ultrasonic stimulation.

The above are only the preferred embodiments of the present invention, and are not intended to limit the present invention, and are in the spirit of the present invention. Any modifications, equivalent substitutions or improvements made within the principles and principles are intended to be included within the scope of the invention.

Claims

Claim

A head-mounted ultrasonic transmission device, comprising: a base that can be placed on a skull of an experimental animal; and a top cover connected to the base, the base having a cavity for receiving a coupling agent, the top The cover is coupled with an ultrasonic transducer having a working surface facing the bottom of the inner cavity; the head-mounted ultrasonic transmission device further includes a fixing device for fixing the base to the skull of the experimental animal, the fixing device including the front end A skull connector embedded in the skull of the experimental animal, the base being attached to the skull of the experimental animal and directly connected to the fixation device or connected by an adhesive.

A head-mounted ultrasonic transmission device according to claim 1, wherein a side of the base near the bottom is provided with a convex structure; or / and a side of the base near the bottom is provided with a groove structure.

The head-mounted ultrasonic transmission device according to claim 2, wherein the cranial connector is a threaded connection, and the adhesive is dental cement;

The convex structure is an everted convex edge, and the adhesive is connected to the skull connecting member and the everted convex edge;

Alternatively, the groove structure is a groove disposed on an outer side surface of the base, and the adhesive is coupled to the skull connector and embedded in the groove.

The head-mounted ultrasonic transmission device according to claim 1, wherein the base is provided with a first protrusion on a side close to a front side of the skull of the experimental animal, or/and the base A second protrusion is provided on a side close to the back side of the skull of the experimental animal. A head-mounted ultrasonic transmission device according to any one of claims 1 to 4, characterized in that the bottom of the base is provided in a curved structure, or / and the bottom of the base is provided with a resilient washer.

The head-mounted ultrasonic transmission device according to any one of claims 1 to 4, wherein the base has an inverted cone shape that is large and small, or/and the top cover is fixed or A detachable connection to the top of the base.

A head-mounted ultrasonic transmission device according to any one of claims 1 to 4, wherein the top cover is coupled to the base by a screw card structure, a snap structure or a threaded structure. [Claim 8] A head-mounted ultrasonic transmission device according to any one of claims 1 to 4, wherein the base is provided with at least one L-shaped lock groove, and the upper end of the L-shaped lock groove a mouthpiece, and the upper end of the L-shaped lock groove is located at an upper end surface of the base, and the top cover is provided with a lock block that can be caught in the L-shaped lock groove.

[Claim 9] A head-mounted ultrasonic transmission device according to any one of claims 1 to 4, wherein a side of the top cover is provided for limiting the ultrasonic transducer a raised structure; or/and, the inside of the top cover is provided with a wire groove for accommodating the cable.

[Claim 10] A head-mounted ultrasonic transmission device according to any one of claims 1 to 4, wherein a side surface of the base is provided for achieving ultrasonic stimulation and electrophysiological collection, electrical stimulation, A catheter structure of at least one of administration, microdialysis, light stimulation, and photo imaging, the catheter structure comprising at least one catheter, the catheter being provided with at least one of an electrode, a capillary tube, or an optical fiber.