WO2018236716A1 - Compositions et procédés permettant d'augmenter le rendement de culture - Google Patents

Compositions et procédés permettant d'augmenter le rendement de culture Download PDF

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WO2018236716A1
WO2018236716A1 PCT/US2018/038013 US2018038013W WO2018236716A1 WO 2018236716 A1 WO2018236716 A1 WO 2018236716A1 US 2018038013 W US2018038013 W US 2018038013W WO 2018236716 A1 WO2018236716 A1 WO 2018236716A1
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composition
hyt
pta
component
plants
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PCT/US2018/038013
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Michael Austin
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Agrinos AS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/10—Fertilisers containing plant vitamins or hormones
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants

Definitions

  • compositions that include a first component comprising nitrogen, potassium, protein, free amino acids, and trace elements and a second component comprising one or more fungicide, such as one or more strobilurin (e.g., picoxystrobin, azoxystrobin,
  • compositions that include a first component comprising nitrogen, potassium, protein, free amino acids, and trace elements and a second component comprising one or more triazole or triazolinthione fungicide (e.g., metconazole, propiconazole, prothioconazole, cyproconazole, tebuconazole, or flutriafol).
  • the first component is HYT B, where HYT B is a liquid fraction obtained from fermentation of chitin- containing materials (such as chitin-containing arthropods or byproducts) with a microbial composition, such as one or more of ATCC Deposit Accession Nos.
  • the second component further includes one or more pyrazole carboxamide fungicide (e.g., benzovindiflupyr or fluxapyroxad).
  • pyrazole carboxamide fungicide e.g., benzovindiflupyr or fluxapyroxad
  • compositions include contacting soil, plants (such as plant foliage, stems, roots, seedlings, or other plant parts), or seeds with a composition disclosed herein.
  • the methods may also include growing treated plants, plant parts, or seeds and/or cultivating plants, plant parts, or seeds in treated soil.
  • the disclosed compositions can be applied to soil, plants, plant parts, or seeds one or more times in a growing season or during various stages of plant growth.
  • FIGS. 1 A and IB are graphs showing wheat yield in bushels/acre (FIG. 1 A) or kg/ha (FIG. IB) in plots treated as shown in Table 2, below.
  • the fungicide alone treatment was standard application of Fortix® fungicide (fluoxastrobin + flutriafol).
  • a and B indicate statistically significant differences, p ⁇ 0.05.
  • Contacting Placement in direct physical association, including both in solid and liquid form. For example, contacting can occur with one or more compositions or compounds and soil, plants, and/or plant parts (such as foliage, stem, seedling, roots, and/or seeds).
  • Fermenting A process that results in the breakdown of complex organic compounds into simpler compounds, for example by microbial cells (such as bacteria and/or fungi). The fermentation process may occur under aerobic conditions, anaerobic conditions, or both (for example, in a large volume where some portions are aerobic and other portions are anaerobic).
  • fermenting includes the enzymatic and/or non-enzymatic breakdown of compounds present in aquatic animals or animal by-products, such as chitin.
  • Fungicide A compound that kills or inhibits growth of fungi or fungal spores.
  • Strobilurin A class of compounds utilized in agriculture as fungicides. Strobilurins are members of the group of quinone outside inhibitors (Qol), which inhibit the respiratory chain at cytochrome b complex III. Exemplary strobilurins include azoxystrobin, picoxystrobin, fluoxastrobin, pyraclostrobin, trifloxystrobin, oryzastrobin, and dimoxystrobin.
  • Triazole or triazolinthione fungicides A class of compounds utilized in agriculture as fungicides. Triazole fungicides include metconazole, tebuconazole, propiconazole, tetraconazole, cyproconazole, difenoconazole, flutriafol, and myclobutanil. Prothioconazole is an exemplary triazolinthione fungicide.
  • compositions that include a first component comprising nitrogen, potassium, protein, free amino acids, and trace elements and a second component comprising one or more fungicides.
  • the second component is one or more strobilurins (e.g., picoxystrobin, azoxystrobin, pyraclostrobin, fluoxastrobin, or flutriafol).
  • the second component is one or more triazole or triazolinthione fungicides (e.g., metconazole, propiconazole, prothioconazole, cyproconazole, or tebuconazole).
  • the second component includes one or more strobilurins and one or more triazole or triazolinthione fungicides. In some embodiments, the second component further includes one or more pyrazole carboxamide fungicide (e.g., benzovindiflupyr or fluxapyroxad).
  • pyrazole carboxamide fungicide e.g., benzovindiflupyr or fluxapyroxad.
  • the composition includes the first component at about 50-95% (such as about 50-75%, about 60-80%, about 75-85%, about 80-95%, or about 80-90%, such as about 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%) and the second component at about 0.5-15% (such as about 0.5-2%, about 1-3%, about 2-5%, about 4-8%, about 5-10%, about 7-12%, or about 1 1 -15%, for example about 0.5%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 1 1%), 12%), 13%), 14%), or 15%) of the composition.
  • the composition may also include other ingredients (including water or other inactive ingredients such as emulsifiers, solvents, carriers, and so on). Exemplary compositions are provided in Table 1, showing the final percentage of HYT B and the indicated fungicide in the composition.
  • compositions shown in Table 1 are diluted in water, for example about 20-fold to 500-fold dilution (such as about 20- to 50-fold dilution, about 30- to 60-fold dilution, about 40- to 80-fold dilution, about 75- to 100-fold dilution, about 90- to 120-fold dilution, about 1 10- to 150-fold dilution, about 125- to 200-fold dilution, about 150- to 250-fold dilution, about 200- to 400-fold dilution, or about 300- to 500-fold dilution).
  • about 20-fold to 500-fold dilution such as about 20- to 50-fold dilution, about 30- to 60-fold dilution, about 40- to 80-fold dilution, about 75- to 100-fold dilution, about 90- to 120-fold dilution, about 1 10- to 150-fold dilution, about 125- to 200-fold dilution, about 150- to
  • compositions are diluted in about 10-30 gallons of water per acre (such as 10-20 gallons/acre, 15- 25 gallons/acre, 20-30 gallons/acre, or 10, 15, 20, 25, or 30 gallons/acre) for example for use in the methods described in Section III.
  • the first component includes nitrogen, potassium, protein, free amino acids, and trace elements (such as calcium, magnesium, zinc, copper, iron, and/or manganese), and optionally, enzymes (such as lactic enzymes, proteases, lipases, and/or chitinases).
  • the first component includes (w/v) about 1-5% total free amino acids (e.g., L- amino acids), about 3-7% protein, about 0.1-2% nitrogen, less than about 0.2% phosphorus, about 0.5-1%) potassium, about 4-8% carbon, about 0.2-1% calcium, less than about 0.2% magnesium, less than about 0.2% sodium, and/or about 0.1-0.4%) sulfur.
  • the first component also includes about 0.01-0.2%) glucosamine (for example, about 0.1% or less or 0.05%) or less).
  • the first component also may contain one or more microbes and/or trace amounts of chitosan and/or chitin.
  • the free amino acids include one or more (such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, or all) of tryptophan, asparagine, aspartic acid, glutamine, glutamic acid, serine, histidine, glycine, threonine, alanine, proline, tyrosine, arginine, valine, methionine, isoleucine, leucine, phenylalanine, and lysine.
  • the first component may optionally include acids (e.g., lactic acid) and/or carbohydrates (e.g., one or more sugars).
  • the first component contains less than 0.5% (w/v) chitosan (e.g., less than 0.4%, less than 0.3%, less than 0.2%, less than 0.1%, less than 0.05%, or less than 0.01%). In other examples, the first component contains less than 0.5% (w/v) chitin (e.g., less than 0.4%, less than 0.3%), less than 0.2%, less than 0.1%>, less than 0.05%>, or less than 0.01%>). In other examples, the first component does not contain detectable amounts of chitosan and/or chitin.
  • the first component of the composition is HYT B.
  • HYT B is the liquid fraction produced from fermentation of chitin-containing materials (such as aquatic animals or aquatic animal byproducts, such as chitin-containing arthropods, for example, shrimp or shrimp byproducts) with a microbial composition that results in biodegradation of chitin. Exemplary methods of producing HYT B are described in U.S. Pat. No. 8,748,124 and International Pat. Publ. No. WO 2016/135698, both of which are incorporated herein by reference in their entirety).
  • the liquid fraction may include components such as protein, amino acids, glucosamine, trace elements (such as calcium, magnesium, zinc, copper, iron, and/or manganese), and/or enzymes (such as lactic enzymes, proteases, lipases, and/or chitinases).
  • the liquid fraction contains (w/v) about 1-5% total amino acids, about 3-7% protein, about 0.1-2%) nitrogen, less than about 0.2% phosphorus, about 0.5-1% potassium, about 4-8% carbon, about 0.2-1% calcium, less than about 0.2% magnesium, less than about 0.2% sodium, and/or about 0.1-0.4% sulfur.
  • the liquid fraction includes about 0.01-0.2%) glucosamine (for example, about 0.1% or less).
  • the liquid fraction also may contain one or more microbes (e.g., from the inoculum used to start the fermentation process) and/or trace amounts of chitosan or chitin (for example, less than 0.5% (w/v) chitosan and/or less than 0.5% (w/v) chitin).
  • HYT B is produced by fermentation of chitin-rich materials (for example, aquatic animals or aquatic animal by-products, insects, or fungi) with a microbial composition.
  • chitin-rich materials for example, aquatic animals or aquatic animal by-products, insects, or fungi
  • one or more microbial compositions are mixed with a chitin- containing biological material to form a mixture and the mixture is fermented. Following fermentation, the mixture is separated into liquid and solid fractions, and optionally a lipid fraction. As discussed above, the liquid fraction is referred to as "HYT B" in some instances.
  • the chitin-rich material is mixed with a microbial composition to form a substantially homogeneous mixture.
  • the microbial composition includes one or more of those described in U.S. Pat. No. 8,748, 124; International Pat. Publ. Nos. WO 2011/157747, WO
  • the microbial composition includes one or more of ATCC Deposit Nos. PTA 10861, PTA-10973, PTA-121750, PTA-121751, PTA-121755, PTA-122728, PTA-123288, PTA-123298, and PTA-123289.
  • the chitin-containing biological material is ground, crushed, minced, milled, or otherwise dispersed prior to mixing with the microbial composition described herein.
  • the mixture contains about 10-50% (such as about 10-20%, about 20-30%, about 30-40%, about 25-40%, for example about 25%, about 30%, about 35%, about 40%, about 45%), or about 50%) chitin-containing material (such as shrimp heads) (w/v) in inoculum containing about 0.1-5% (such as about 0.1-1%, about 0.5-2%, about 1-2%, about 2-3%, about 0.1%, about 0.2%, about 0.3%, about 0.5%, about 0.8%, about 1%, about 1.25%, about 1.5%, about 1.75%, about 2%), about 2.5%, about 3%, about 4%, or about 5%) of the microbial composition (v/v).
  • about 10-50% such as about 10-20%, about 20-30%, about 30-40%, about 25-40%, for example about 25%, about 30%, about 35%, about 40%, about 45%
  • chitin-containing material such as shrimp heads
  • inoculum containing about 0.1-5% (such as about 0.1
  • the inoculum, chitin-containing biological material, and a sugar (or other carbon source) are mixed together, for example by stirring or agitation.
  • one or more of the microbes in the microbial composition is optionally activated prior to mixing with the chitin-containing biological material and fermentation. Activation is not required for the methods disclosed herein. Adjustments to the time and/or temperature of the fermentation can be made by one of skill in the art, depending on whether the microbes are activated prior to fermentation.
  • Activation of the microbe(s) can be by incubating an inoculum of the microbial composition with a carbon source (such as a sugar, for example, glucose, sucrose, fructose, or other sugar) at a temperature and for a sufficient period of time for the microbes to grow.
  • a carbon source such as a sugar, for example, glucose, sucrose, fructose, or other sugar
  • an inoculum of the microbes (such as a microbial composition described herein) has a concentration of about 0.05-5% v/v (for example, about 0.5-5%, about 0.5-2%, about 1-2%, or about 2-3%) in a liquid medium.
  • the inoculum is diluted in a solution containing about 0.1-1% sugar (for example, about 0.1-0.5%, about 0.1-0.3%, about 0.2-0.6%, or about 0.5-1%, such as about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, or about 1%)) and incubated at ambient temperatures, for example about 20-40°C (such as about 20°C, about 25°C, about 30°C, about 35°C, or about 40°C) for about 1-5 days (such as about 24 hours, about 48 hours, about 72 hours, about 96 hours, or about 120 hours).
  • sugar for example, about 0.1-0.5%, about 0.1-0.3%, about 0.2-0.6%, or about 0.5-1%, such as about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, or about 1%)
  • ambient temperatures for example about 20-40°C (such as about
  • activation of the microbe(s) can be activated by incubating an inoculum of the microbial composition at a temperature and for a sufficient period of time for the microbes to grow, for example, incubation at about 20-40°C (such as about 25-35°C) for 12 hours to 5 days (such as 1 -4 days or 2-3 days).
  • the microbes are considered to be activated when the culture reaches an optical density of >0.005 at 600 nm.
  • the mixture is fermented.
  • the pH of the mixture is measured prior to fermentation.
  • the pH is adjusted to a selected range (e.g., pH about 3 to about 4 or about 3.5 to 4), if necessary, prior to fermentation.
  • the mixture is incubated at a temperature of about 20-40°C (for example, about 30°-36°C, such as about 30°C, about 31°C, about 32°C, about 33°C, about 34°C, about 35°C, about 36°C, about 37°C, about 38°C, about 39°C, or about 40°C) for about 1 -30 days (such as about 3-28 days, about 7-21 days, about 3, 5, 7, 10, 14, 16, 20, 24, 28, or 30 days).
  • the mixture is agitated periodically (for example, non-continuous agitation).
  • the mixture is agitated for a period of time every 1 -7 days, for example every 1, 2, 3, 4, 5, 6, or 7 days.
  • the fermentation proceeds until the titratable acidity (TTA) is about 3-5% and the pH is about 4-5.
  • TTA titratable acidity
  • the resulting fermented mixture is separated into at least solid and liquid fractions.
  • the fermentation is passed from the tank to settling equipment.
  • the liquid is subsequently decanted and centrifuged.
  • the fermented mixture is centrifuged at 1250 rpm (930xg) for 15 minutes at about 5°C to obtain liquid and lipid (e.g., pigment) fractions.
  • biodegradation process can be stored at ambient temperature.
  • a sugar is added to the liquid fraction, for example at 1-10% v/v.
  • the second component of the disclosed compositions is one or more strobilurin.
  • Strobilurins are a class of organic compounds that are utilized in agriculture as fungicides.
  • Exemplary strobilurins include azoxystrobin, picoxystrobin, fluoxastrobin, pyraclostrobin, trifloxystrobin, oryzastrobin, and dimoxystrobin.
  • strobilurins are commercially available under a variety of trade names.
  • the second component of the disclosed compositions is picoxystrobin (e.g., Aproach® fungicide, Dow Chemical).
  • the second component of the disclosed composition is one or more triazole or triazolinthione fungicide, such as metconazole (5-[(4-chlorophenyl)methyl]-2,2- dimethyl-l-(l,2,4-triazol-l-ylmethyl)cyclopentan-l-ol, e.g., Caramba® fungicide, BASF), prothioconazole (2-[2-(l-chlorocyclopropyl)-3-(2-chlorophenyl)-2-hydroxypropyl]-lH-l,2,4- triazole-3-thione), tebuconazole (l-(4-chlorophenyl)-4,4-dimethyl-3-(l,2,4-triazol-l- ylmethyl)pentan-3-ol, e.g., Folicur® fungicide), propiconazole, flutriafol, or two or more thereof.
  • metconazole 5-[(4-chlor
  • the second component includes a combination of a strobilurin and/or a triazole or triazolinthione fungicide, optionally with additional components, for example, azoxystrobin plus propiconazole (e.g., Quilt® fungicide, Syngenta), prothioconazole plus tebuconazole (e.g., Prosaro® fungicide, Bayer), fluxapyroxad plus pyraclostrobin plus
  • azoxystrobin plus propiconazole e.g., Quilt® fungicide, Syngenta
  • prothioconazole plus tebuconazole e.g., Prosaro® fungicide, Bayer
  • the second component further includes one or more pyrazole carboxamide fungicides, such as benzovindiflupyr or fluxapyroxad.
  • the second component includes prothioconazole and tebuconazole; azoxystrobin and propiconazole; metconazole; benzovindiflupyr, azoxystrobin, and propiconazole; tebuconazole; fluxapyroxad, pyraclostrobin, and propiconazole; picoxystrobin and cyproconazole; fluoxastrobin and flutriafol; or picoxystrobin.
  • Exemplary compositions are provided in Table 1.
  • the disclosed compositions can be used to treat soil, plants, or plant parts (such as roots, stems, foliage, seeds, or seedlings).
  • treatment with the disclosed compositions improves plant growth, improves stress tolerance, and/or increases crop yield.
  • the methods include contacting soil, plants (such as plant foliage, stems, roots, seedlings, or other plant parts), or seeds with a composition disclosed herein.
  • the methods may also include growing treated plants, plant parts, or seeds and/or cultivating plants, plant parts, or seeds in treated soil.
  • the disclosed compositions can be applied to soil, plants, plant parts, or seeds one or more times in a growing season or during various stages of plant growth (for example, 1, 2, 3, 4, 5, or more times).
  • the composition is applied at various stages of plant growth, depending on the fungus to be controlled and/or the plant to be treated.
  • the composition is applied to wheat at boot stage (Feekes stage 10) to control stripe rust (caused by Puccinia striiformis).
  • the composition is applied to wheat at heading (Feekes stage 10.5) to control head scab ⁇ Fusarium head blight).
  • Appropriate timing of application(s) of the composition can be selected based on plant, fungus, weather, or other conditions.
  • the amount of the composition to be applied is calculated and the composition is diluted in water (or in some examples, water plus liquid fertilizer) to an amount sufficient to spray or irrigate the area to be treated.
  • the composition can be applied at the time of seed planting at a rate of 0.5-2 liters per acre (such as about 0.5, 1, 1.5, or 2 liters per acre) or 1-3 pints per acre (such as about 1, 1.5, 2, 2.5, or 3 pints per acre). In one example, the composition is applied at 1 liter per acre or 1 pint/acre. In other examples, the composition is diluted in water and applied at 10-30 gallons/acre. The composition can also be applied to the soil ⁇ e.g., near the plant roots) or plant one or more times during growth, in the same or a different amount.
  • compositions can be delivered in a variety of ways at different developmental stages of the plant, depending on the cropping situation and agricultural practices.
  • the disclosed compositions can be delivered through drip irrigation at low concentration as seedlings or transplants are being established, delivered in flood irrigation, or dispensed in overhead or drip irrigation in greenhouses to seedlings or established plants, or alternatively are applied individually.
  • a disclosed composition is injected into overhead water spray irrigation systems or drip irrigation lines over the course of the plant' s growth.
  • the first component and the second component can be applied to soil, plants (such as plant foliage, stems, roots, seedlings, or other plant parts), or seeds individually, rather than as a mixture. If the components are applied separately, they are applied within hours to days of one another, to preserve benefits provided by applying the disclosed compositions. In some examples, the first component and second component are applied to soil, plants, plant parts, or seeds within 2 hours to 3 days of one another (such as within 2-12 hours, 4-18 hours, 6-24 hours, 12-36 hours, 24-60 hours, or 48-72 hours). In these examples, the first component and the second component can be applied in either order. In one example, the first component is applied before the second component.
  • additional compositions are also applied to the soil, plants, plant parts, or seeds, either simultaneously or sequentially (e.g., before or after) with the first or second components.
  • a microbial composition such as HYT A
  • HYT A is applied to the soil, plants, plant parts, or seeds.
  • HYT A is applied to the soil, plants, plant parts, or seeds (alone or in combination with HYT B) at least once prior to application of the disclosed compositions.
  • HYT A is a microbial composition (ATCC Deposit No. PTA-10973) described in International Pat. Publ. No. WO 201 1/157747, which is incorporated here by reference in its entirety.
  • composition increases plant growth (such as overall plant size, amount of foliage, root number, root diameter, root length, production of tillers, fruit production, pollen production, or seed production) by at least about 5% (for example, at least about 10%, at least about 30%, at least about 50%, at least about 75%, at least about 100%, at least about 2-fold, at least about 3-fold, at least about 5- fold, at least about 10-fold, or more).
  • the disclosed methods result in increased crop production of about 10-75%> (such as about 20-60% or about 30-50%) compared to untreated crops.
  • controls include the same agronomic practices without addition of one or more components of the composition, performed in parallel.
  • crop yield is increased by the composition by maintaining and/or increasing plant health and/or growth and/or reducing stress on the plant, by action of the first component simultaneously or substantially simultaneously (e.g., within hours to days, such as within 2 hours to 3 days) with the action of the second component.
  • the effect of the disclosed compositions on plant growth or crop production is synergistic, for example, compared with application of the first component or second component alone.
  • the disclosed methods can be used in connection with any crop.
  • Exemplary crops include, but are not limited to alfalfa, almond, banana, barley, broccoli, canola, carrots, citrus and orchard tree crops, corn, cotton, cucumber, flowers and ornamentals, garlic, grapes, hops, horticultural plants, leek, melon, oil palm, oil seed rape, onion, peanuts and legumes, pineapple, poplar, pine and wood-bearing trees, potato, raspberry, rice, sesame, sorghum, soybean, squash, strawberry, sugarcane, sugarbeets, sunflower, tomato, turf and forage grasses, watermelon, wheat, and eucalyptus.
  • wheat is treated as described herein.
  • the disclosed methods are used with row crops, such as corn, rice, soybean, cotton, or sorghum.
  • This example describes the effect on yield of treatment of wheat with HYT B and picoxystrobin.
  • This example describes exemplary methods that can be used to evaluate efficacy of HYT B and fungicide combination treatments on wheat.
  • methods that deviate from these specific methods can also be used to evaluate HYT B and fungicide combination treatments, for example, utilizing different combinations and/or amounts of the agents or other crops or plants.
  • Exemplary treatments during the growing season are shown in Table 3. Treatments may be selected from those in Table 3, and a trial may not include all of the listed treatments. However, each trial includes normal routine and HYT B alone as controls. Each treatment may be carried out in two or more replicates.
  • the plots are scored for head scab (1-6 scale) five days after application of fungicide or HYT B + fungicide. Yield and harvest weight from each plot are measured at harvest. An increase in yield and/or harvest weight compared to a control (such as HYT B alone) indicates efficacy of the treatment.

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne des compositions qui comprennent un premier constituant comprenant de l'azote, du potassium, une protéine, des acides aminés libres et des oligo-éléments et un second constituant comprenant un ou plusieurs fongicides, tels qu'un ou plusieurs fongicides à base de strobilurine et/ou un ou plusieurs fongicides à base de triazole ou de triazolinthione. Selon certains exemples, le premier constituant est l'HYT B, l'HYT B étant une fraction liquide obtenue à partir de la fermentation d'arthropodes contenant de la chitine avec une composition microbienne. L'invention concerne également des procédés d'utilisation des compositions. De tels procédés comprennent la mise en contact du sol, de plantes (telles que le feuillage, les tiges, les racines, les semis des plantes ou d'autres parties végétales), ou de graines avec une composition décrite dans la description.
PCT/US2018/038013 2017-06-19 2018-06-18 Compositions et procédés permettant d'augmenter le rendement de culture WO2018236716A1 (fr)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11230504B2 (en) * 2017-06-16 2022-01-25 Fluid Quip Technologies, Llc Method and system for producing a fertilizer or herbicide from a whole stillage byproduct produced in a corn dry milling process

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120045497A1 (en) * 2009-01-09 2012-02-23 Syngenta Crop Protection, Llc Pesticidal combinations
US20140364310A1 (en) * 2011-12-22 2014-12-11 Vive Crop Protection Inc. Strobilurin formulations
CN104705317A (zh) * 2015-02-13 2015-06-17 广西壮族自治区农业科学院农产品质量安全与检测技术研究所 含苯并烯氟菌唑与三唑类杀菌剂组合物的超低容量液剂
WO2016135699A1 (fr) * 2015-02-27 2016-09-01 Agrinos AS Consortiums microbiens

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120045497A1 (en) * 2009-01-09 2012-02-23 Syngenta Crop Protection, Llc Pesticidal combinations
US20140364310A1 (en) * 2011-12-22 2014-12-11 Vive Crop Protection Inc. Strobilurin formulations
CN104705317A (zh) * 2015-02-13 2015-06-17 广西壮族自治区农业科学院农产品质量安全与检测技术研究所 含苯并烯氟菌唑与三唑类杀菌剂组合物的超低容量液剂
WO2016135699A1 (fr) * 2015-02-27 2016-09-01 Agrinos AS Consortiums microbiens

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11230504B2 (en) * 2017-06-16 2022-01-25 Fluid Quip Technologies, Llc Method and system for producing a fertilizer or herbicide from a whole stillage byproduct produced in a corn dry milling process

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