WO2018214317A1 - 一种生物发酵过滤系统 - Google Patents
一种生物发酵过滤系统 Download PDFInfo
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- WO2018214317A1 WO2018214317A1 PCT/CN2017/099149 CN2017099149W WO2018214317A1 WO 2018214317 A1 WO2018214317 A1 WO 2018214317A1 CN 2017099149 W CN2017099149 W CN 2017099149W WO 2018214317 A1 WO2018214317 A1 WO 2018214317A1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
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- the present application relates to a filtration system, and more particularly to a biological fermentation filtration system.
- microorganisms such as E. coli, yeast, etc.
- the fermentation liquid is separated by the bacterial liquid, and the supernatant of the fermentation liquid is obtained.
- the conventional separation method is to enrich the cells through a tubular continuous flow centrifuge and separate the fermentation supernatant.
- it also has some problems: after the separation of the bacteria liquid, it is difficult to control whether the supernatant still has the bacteria; the volume of the centrifuge drum is small, the machine should be shut down frequently, the bacteria residue is removed, and the operation is intermittent, which affects the efficiency; Large, noisy.
- the hollow fiber membrane filtration system designed for microfiltration or ultrafiltration concentration suitable for laboratory scale or pilot scale in China has the problem that the hollow fiber membrane module assembled by the fixed frame is inconvenient to disassemble and cannot be According to different preparation processes, the hollow fiber membrane modules of different specifications are flexibly replaced; the storage tanks are separately separated, and the movement of the pump body is inconvenient.
- the purpose of the present application is to provide a biological fermentation filtration system in view of the above problems.
- the utility model has the advantages of simple structure, convenient disassembly and easy cleaning, and is a movable biological fermentation filtration system.
- the hollow fiber membrane module of different specifications can be replaced, and the microbial fermentation liquid can be microfiltered and sterilized, and can also be used for sterilization.
- the filtrate product was concentrated by ultrafiltration.
- the biological fermentation filtration system of the present application comprising a tank body, a peristaltic pump, two hollow fiber membrane modules, a first three-way valve, a second three-way valve, a third three-way valve, and a first a pipe, a second pipe, a third pipe, a fourth pipe, a fifth pipe, and a sixth pipe, wherein the return ports of the two hollow fiber membrane modules are connected by the first pipe, intersecting the fifth pipe, and two hollow fiber membranes
- the feed port of the module is connected through the second pipe and merges with the fourth pipe, and the filtrate outlets of the two hollow fiber membrane modules are connected through the third pipe to meet the sixth pipe, and the bottom of the tank is provided with the first three a valve, the first port of the first three-way valve is connected to the tank body, and the second port of the first three-way valve is connected to the second pipe through the fourth pipe through the peristaltic pump, and the third port of the first three-way valve serves as a row Feed port
- the utility model has the advantages of simple structure, convenient disassembly, easy cleaning and movable biological fermentation filtration system, and replacement of hollow fiber membrane modules of different specifications, microfiltration sterilization filtration of the microbial fermentation liquid, and filtration liquid after sterilization
- the product was concentrated by ultrafiltration.
- the microfiltration membrane of the hollow fiber membrane module can be used for microfiltration to remove engineering bacteria (such as Escherichia coli, yeast, etc.).
- the ultrafiltration membrane of the hollow fiber membrane module can be used to concentrate biomultipolymers (such as polyglutamic acid, plant polysaccharides, etc.) in different molecular weight ranges.
- Figure 1 is a schematic structural view of the present application
- FIG. 2 is a schematic structural view of a connecting portion corresponding to a first opening of an upper portion of a can body
- FIG. 3 is a schematic structural view of a connecting portion corresponding to a second opening of an upper portion of the can body
- Figure 4 is a schematic view showing the structure of the connecting portion corresponding to the bottom opening of the can body.
- the biological fermentation filtration system of the present application comprises a tank body 1, a peristaltic pump 2, two hollow fiber membrane modules 3, a first three-way valve 51, a second three-way valve 52, a third three-way valve 53, and a first conduit. 61.
- the bottom of the tank body 1 is provided with a first three-way valve 51, the first interface of the first three-way valve 51 is connected with the tank body 1, and the second interface of the first three-way valve 51 is passed through
- the fourth pipe 71 is connected to the second pipe 62 via the peristaltic pump 2, and the third port of the first three-way valve 51 serves as a discharge port.
- the top of the can body 1 is provided with a can lid 15.
- a can lid 15 In this embodiment, one half of the above-mentioned can lid 15 is fixed, and half of the can lid 15 can be flexibly opened.
- the can body 1 is a stainless steel can body, and a first opening is formed in the upper portion of the side wall, and the second sanitary ball valve 9, the second three-way valve 52 and the third sanitary ball valve 10 are mounted on the first opening.
- the second sanitary ball valve 9 is connected to the first opening, and the other side of the second sanitary ball valve 9 is connected to the second three-way valve 52.
- the second three-way valve 52 is horizontally opened to the fifth pipe 72, and the second three-way valve 52 is vertically opened.
- the three sanitary ball valve 10 serves as a discharge port. See Figure 2.
- the upper portion of the side wall of the can body 1 is provided with a second opening, and the second opening is provided with a fourth sanitary ball valve 11, a third three-way valve 53, and a fifth sanitary ball valve 12.
- the fourth sanitary ball valve 11 is connected to the second opening, the other side of the fourth sanitary ball valve 11 is connected to the third three-way valve 53, and the third three-way valve 53 is horizontally open.
- a six-pipe 73, a third three-way valve 53 is vertically open to the fifth sanitary ball valve 12 as a discharge port. See Figure 3.
- the fourth duct 71 is connected to the second duct 62 via the peristaltic pump 2, and the other end of the fourth duct 71 is connected to the first sanitary ball valve 8.
- the first duct 61, the second duct 62, and the third duct 63 are PVC pipes.
- the fourth duct 71, the fifth duct 72, and the sixth duct 73 are silicone tubes.
- the bottom of the can body 1 is provided with a first three-way valve 51, the first interface of the first three-way valve 51 is connected to the can body 1, and the second interface of the first three-way valve 51 is passed through the fourth
- the pipe 71 is connected to the second pipe 62 via the peristaltic pump 2, and the third port of the first three-way valve 51 serves as a larger discharge port. See Figure 4.
- the bottom of the can body 1 is provided with three supporting legs, and the three supporting legs are respectively provided with three universal wheels 14, and the tank wall of the can body 1 is provided with a handle.
- the hollow fiber membrane module 3 is provided with a microfiltration hollow fiber membrane module or an ultrafiltration hollow fiber membrane module.
- the filtration membrane diameter of the microfiltration membrane provided in the hollow fiber membrane module 3 is 0.15 ⁇ m to 0.85. Two 0.2 ⁇ m apertures were chosen.
- the hollow fiber membrane module 3 passes through the PVC pipe to respectively feed the inlet port 31, the return port 32, and the filtrate outlet port 33, and communicates in parallel to the inlet port main pipe, the return port main pipe, and the filter liquid outlet main pipe.
- the inlet pipe and the return port main pipe are equipped with a pressure gauge, and the pressure gauge ranges from 0.1 to 0.6 MPa.
- the working principle of the application is: moving the filter system to the side of the fermenter, opening the upper cover of the tank body 1, and passing the fermented E. coli fermentation liquid through the tank pressure to the tank body 1 through the pipeline, opening the first The sanitary ball valve 8, the fifth sanitary ball valve 12, the third sanitary ball valve 10, the fourth sanitary ball valve 11, and the sixth sanitary ball valve 13 are closed.
- the sanitary ball valve second sanitary ball valve 9 is in an adjustable state for controlling the pressure gauge The pointer reading does not exceed 0.2 MPa.
- the microfiltrate is discharged through the outlet of the fifth sanitary ball valve 12 of the hygienic ball valve in an open state to obtain a protein or polypeptide product. Fermentation supernatant.
- the first sanitary ball valve 8, the third sanitary ball valve 10, the fifth sanitary ball valve 12, the fourth sanitary ball valve 11, the sixth sanitary ball valve 13, and the second sanitary ball valve 9 are in an adjustable state, and the water is poured.
- the residual waste liquid in the hollow fiber membrane module 3 is washed away.
- the first sanitary ball valve 8 After washing away the residual waste liquid in the hollow fiber membrane module 3, the first sanitary ball valve 8, the fourth sanitary ball valve 11, the third sanitary ball valve 10, the fifth sanitary ball valve 12, the sixth sanitary ball valve 13, and the second sanitary ball valve are opened. 9 is in an adjustable state and then rinsed back with an acidic or alkaline wash for about 1 hour.
- the first sanitary ball valve 8 After refluxing with an acidic or alkaline washing solution for about 1 hour, the first sanitary ball valve 8, the third sanitary ball valve 10, the fifth sanitary ball valve 12, the fourth sanitary ball valve 11, the sixth sanitary ball valve 13, the second sanitary
- the ball valve 9 is in an adjustable state, and the fresh water is poured into the can body to wash away the residual acidic or alkaline washing liquid in the hollow fiber membrane module 3.
- the residual acidic or alkaline washing liquid in the hollow fiber membrane module 3 is washed away with water, and the pH value of the water is about 7 by the pH reagent, the first sanitary ball valve 8 is opened, the fourth sanitary ball valve 11 is closed, and the third sanitary ball valve is closed.
- the fifth sanitary ball valve 12, the sixth sanitary ball valve 13, the second sanitary ball valve 9 is in an adjustable state, and then refluxed with a suitable concentration of sodium hypochlorite, so that the hollow fiber membrane module 3 is filled with sodium hypochlorite preservation solution, that is, the cleaning is completed. Save your work.
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Abstract
本发明提供了一种生物发酵过滤系统,其特征在于包括罐体、蠕动泵、两个中空纤维膜组件、第一三通阀、第二三通阀、第三三通阀、第一管道、第二管道、第三管道、第四管道、第五管道和第六管道,其中两个中空纤维膜组件的出料口通过第一管道连接,交汇于第五管道,两个中空纤维膜组件的进料口通过第二管道连接,交汇于第四管道,两个中空纤维膜组件的滤过液出口通过第三管道连接,交汇于第六管道,罐体底部装设有第一三通阀,第一三通阀的第一接口与罐体连接,第一三通阀的第二接口通过第四管道经蠕动泵与第二管道连接连接,第一三通阀的第三接口作为排料口。
Description
相关申请的交叉引用
本申请要求于2017年05月25日提交中国专利局的申请号为CN201720594618.4、名称为“一种生物发酵过滤系统”的中国专利申请的优先权,其全部内容通过引用结合在本申请中。
本申请涉及一种过滤系统,尤其涉及一种生物发酵过滤系统。
随着基因工程技术的发展,通过改造微生物基因,让工程菌(如大肠杆菌、酵母菌等)直接分泌蛋白质或多肽等产物到发酵液上清液中,已成为一种常规手段。对发酵液进行菌液分离,获取发酵液上清液,传统的分离方法是通过管式连续流离心机,富集菌体,分离出发酵上清液。但其也存在一些问题:菌液体分离后,很难把控上清液是否还残留有菌体;离心机转鼓容积小,要频繁停机,清除菌渣,间歇操作,影响效率;运行功耗大,噪音大。
目前在国内针对适合实验室规模或中试规模做微滤除菌或超滤浓缩所设计的中空纤维膜过滤系统,其存在的问题是:固定框架组装的中空纤维膜组件,拆卸不方便,不能根据不同的制备工艺,灵活地去更换不同规格的中空纤维膜组件;储料罐也是单独分开,连接泵体后,造成移动不方便。
发明内容
本申请的目的在于考虑上述问题而提供一种生物发酵过滤系统。本申请结构简单,拆卸方便,易于清洗,是一种可移动的生物发酵过滤系统,更换不同规格的中空纤维膜组件,可对微生物发酵液进行微滤除菌过滤,亦可对除菌后的滤过液产物进行超滤浓缩。
本申请的技术方案是:本申请的生物发酵过滤系统,包括有罐体、蠕动泵、两个中空纤维膜组件、第一三通阀、第二三通阀、第三三通阀、第一管道、第二管道、第三管道、第四管道、第五管道和第六管道,其中两个中空纤维膜组件的回料口通过第一管道连接,交汇于第五管道,两个中空纤维膜组件的进料口通过第二管道连接,交汇于第四管道,两个中空纤维膜组件的滤过液出口通过第三管道连接,交汇于第六管道,罐体的底部装设有第一三通阀,第一三通阀的第一接口与罐体连接,第一三通阀的第二接口通过第四管道经蠕动泵与第二管道连接,第一三通阀的第三接口作为排料口。
本申请结构简单、拆卸方便、易于清洗、可移动的生物发酵过滤系统,更换不同规格的中空纤维膜组件,可对微生物发酵液进行微滤除菌过滤,亦可对除菌后的滤过液产物进行超滤浓缩。另外,中空纤维膜组件的微滤膜可用于微滤去除工程菌(如大肠杆菌、酵母菌等)。所述中空纤维膜组件的超滤膜可用于浓缩不同分子量区间的生物多聚合物(如多聚谷氨酸、植物多糖等)。
图1是本申请的结构示意图;
图2是罐体上部的第一开口对应的连接部分结构示意图;
图3是罐体上部的第二开口对应的连接部分结构示意图;
图4是罐体底部开口对应的连接部分结构示意图。
下面结合附图和具体实施例对本申请作进一步说明,但本申请的保护范围不限于此。
本申请的生物发酵过滤系统,包括有罐体1、蠕动泵2、两个中空纤维膜组件3、第一三通阀51、第二三通阀52、第三三通阀53、第一管道61、第二管道62、第三管道63、第四管道71、第五管道72和第六管道73,其中两个中空纤维膜组件3的回料口32通过第一管道61连接,交汇于第五管道72,两个中空纤维膜组件3的进料口31通过第二管道62连接,交汇于第四管道71,两个中空纤维膜组件3的滤过液出口33通过第三管道63连接,交汇于第六管道73,罐体1的底部装设有第一三通阀51,第一三通阀51的第一接口与罐体1连接,第一三通阀51的第二接口通过第四管道71经蠕动泵2与第二管道62连接,第一三通阀51的第三接口作为排料口。
此外,上述罐体1的顶部设有罐盖15。本实施例中,上述罐盖15的一半固定,一半能可灵活开盖。
本实施例中,上述罐体1是不锈钢罐体,其侧壁上部设有第一开口,第一开口上装第二卫生球阀9、第二三通阀52及第三卫生球阀10。第二卫生球阀9接第一开口,第二卫生球阀9另一侧接第二三通阀52,第二三通阀52水平开口接第五管道72,第二三通阀52垂直开口接第三卫生球阀10,作为排料口。见图2。
本实施例中,上述罐体1侧壁上部设有第二开口,第二开口上装设有第四卫生球阀11、第三三通阀53及第五卫生球阀12。第四卫生球阀11接第二开口,第四卫生球阀11另一侧接第三三通阀53,第三三通阀53水平开口接第
六管道73,第三三通阀53垂直开口接第五卫生球阀12,作为排料口。见图3。
本实施例中,上述第四管道71经蠕动泵2与第二管道62连接,第四管道71另一端与第一卫生球阀8连接。
本实施例中,上述第一管道61、第二管道62和第三管道63是PVC管。
本实施例中,上述第四管道71、第五管道72和第六管道73是硅胶管。
本实施案例中,上述罐体1的底部装设有第一三通阀51,第一三通阀51的第一接口与罐体1连接,第一三通阀51的第二接口通过第四管道71经蠕动泵2与第二管道62连接,第一三通阀51的第三接口作为较大的排料口。见图4。
本实施例中,上述罐体1的底部装设有三根支撑脚,三根支撑脚分别装设有三个万向轮14,罐体1的罐壁装设有拉手。
本实施例中,上述中空纤维膜组件3装设有微滤中空纤维膜组件或超滤中空纤维膜组件。
本实施例中,上述中空纤维膜组件3所设微滤膜的过滤孔径为:0.15μm~0.85。选用两根0.2μm孔径。
所述中空纤维膜组件3,都通过PVC管分别将进料口31,回料口32,滤过液出口33,并联相通交汇到进料口总管,回料口总管,滤过液出口总管。进料口总管和回料口总管安装有压力表,压力表范围在0.1~0.6MPa。
本申请的工作原理是:把过滤系统移动至发酵罐旁,打开罐体1的上盖,将已经发酵结束的大肠杆菌发酵液通过罐内压力,经管道传送至罐体1内,打开第一卫生球阀8、第五卫生球阀12,关闭第三卫生球阀10、第四卫生球阀11、第六卫生球阀13,过滤过程中,卫生球阀第二卫生球阀9处于可调节状态,用于控制压力表指针读数不超过0.2MPa。经过蠕动泵2的输送,微滤液经打开状态的卫生球阀第五卫生球阀12出口流出,得含有蛋白质或多肽产物
的发酵上清液。
过滤结束后,打开第一卫生球阀8,第三卫生球阀10,第五卫生球阀12,关闭第四卫生球阀11,第六卫生球阀13,第二卫生球阀9处于可调节状态,将清水倒入罐体内,冲走中空纤维膜组件3内的残留废液。
冲走中空纤维膜组件3内的残留废液后,打开第一卫生球阀8,第四卫生球阀11,关闭第三卫生球阀10,第五卫生球阀12,第六卫生球阀13,第二卫生球阀9处于可调节状态,再用酸性或碱性洗液进行回流冲洗约1小时。
用酸性或碱性洗液进行回流冲洗约1小时后,打开第一卫生球阀8,第三卫生球阀10,第五卫生球阀12,关闭第四卫生球阀11,第六卫生球阀13,第二卫生球阀9处于可调节状态,将清水倒入罐体内,冲走中空纤维膜组件3内的残留的酸性或碱性洗液。
用清水冲走中空纤维膜组件3内的残留的酸性或碱性洗液,用pH试剂测试水质pH值约为7左右,打开第一卫生球阀8,第四卫生球阀11,关闭第三卫生球阀10,第五卫生球阀12,第六卫生球阀13,第二卫生球阀9处于可调节状态,再用适宜浓度的次氯酸钠进行回流冲洗,让中空纤维膜组件3内充满次氯酸钠保存液,即完成清洗和保存工作。
Claims (11)
- 一种生物发酵过滤系统,其特征在于包括有罐体、蠕动泵、两个中空纤维膜组件、第一三通阀、第二三通阀、第三三通阀、第一管道、第二管道、第三管道、第四管道、第五管道和第六管道,其中两个中空纤维膜组件的回料口通过第一管道连接,交汇于第五管道,两个中空纤维膜组件的进料口通过第二管道连接,交汇于第四管道,两个中空纤维膜组件的滤过液出口通过第三管道连接,交汇于第六管道,罐体的底部装设有第一三通阀,第一三通阀的第一接口与罐体连接,第一三通阀的第二接口通过第四管道经蠕动泵与第二管道连接,第一三通阀的第三接口作为排料口。
- 根据权利要求1所述的生物发酵过滤系统,其特征在于所述罐体的顶部设有罐盖。
- 根据权利要求2所述的生物发酵过滤系统,其特征在于所述罐盖的一半固定,一半能灵活开盖。
- 根据权利要求1所述的生物发酵过滤系统,其特征在于所述罐体侧壁上部设有第一开口,第一开口上装设有第二卫生球阀、第二三通阀及第三卫生球阀,第二卫生球阀接罐体侧壁的第一开口,第二卫生球阀的另一侧接第二三通阀,第二三通阀的水平开口接第五管道,第二三通阀的垂直开口接第三卫生球阀,作为排料口。
- 根据权利要求1所述的生物发酵过滤系统,其特征在于所述罐体侧壁上部设有第二开口,第二开口上装设有第四卫生球阀、第三三通阀及第五卫生球阀,第四卫生球阀接罐体侧壁上的第二开口,第四卫生球阀的另一侧接第三三通阀,第三三通阀的水平开口接第六管道,第三三通阀的垂直开口接第五卫生 球阀,作为排料口。
- 根据权利要求1所述的生物发酵过滤系统,其特征在于所述第四管道的另一端与第一卫生球阀连接。
- 根据权利要求1至6任一项所述的生物发酵过滤系统,其特征在于所述第一管道、第二管道和第三管道是PVC管;所述第四管道、第五管道和第六管道是硅胶管。
- 根据权利要求7所述的生物发酵过滤系统,其特征在于所述罐体(1)的底部装设有三根支撑脚,三根支撑脚分别装设有三个万向轮(14),罐体(1)的罐壁装设有拉手。
- 根据权利要求7所述的生物发酵过滤系统,其特征在于所述中空纤维膜组件为微滤中空纤维膜组件或超滤中空纤维膜组件。
- 根据权利要求9所述的生物发酵过滤系统,其特征在于所述中空纤维膜组件所设微滤膜的过滤孔径为:0.001μm~0.12μm。
- 根据权利要求9所述的生物发酵过滤系统,其特征在于所述中空纤维膜组件所设超滤膜过滤孔径为:0.15μm~0.85μm。
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CN201873680U (zh) * | 2010-07-07 | 2011-06-22 | 中国石油化工股份有限公司 | 基于渗透汽化膜的乙醇发酵与分离耦合的系统装置 |
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