WO2018136018A1 - Method for increasing methane yield in anaerobic digesters by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats. - Google Patents
Method for increasing methane yield in anaerobic digesters by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats. Download PDFInfo
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- WO2018136018A1 WO2018136018A1 PCT/TR2017/050035 TR2017050035W WO2018136018A1 WO 2018136018 A1 WO2018136018 A1 WO 2018136018A1 TR 2017050035 W TR2017050035 W TR 2017050035W WO 2018136018 A1 WO2018136018 A1 WO 2018136018A1
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- goats
- hornless
- rumen fluid
- cocktail
- cebac
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Definitions
- This invention is related to a method for increasing methane yield in anaerobic digesters by means of treating lignocellulosic biomass, by adding a cellulolytic bacteria cocktail (cebac-g) prepared from rumen fluid of hornless goats.
- a cellulolytic bacteria cocktail (cebac-g) prepared from rumen fluid of hornless goats.
- this invention is related to a method for increasing methane yield in anaerobic digesters, by adding a cellulolytic bacteria cocktail (cebac-g) prepared from rumen fluid of hornless goats (Naturally polled goats), into anaerobic digesters that has been developed in order to increase methane yield in a system by increasing the hydrolysis efficiency at a maximum level, in anaerobic digesters treating lignocellulosic biomass, such as animal manure, agricultural waste, energy plants, food residue, sewage sludge, household wastewater and industrial wastewater.
- a cellulolytic bacteria cocktail a cellulolytic bacteria cocktail
- hornless goats Naturalally polled goats
- the recalcitrant characteristic of the biomass makes the hydrolysis of these biomasses is harder and there is a need to apply different strategies such as physical/chemical/biological pre-treatment during anaerobic digestion.
- the carrying out of hydrolysis with low efficiency prevents from benefiting efficiently from the biomass' energy potential of digesters which also decreases the system methane yield.
- the United Nations patent document filed on the date of 23.01.2009 numbered US2009137016 of the known state of the art describes a method which comprises a phase in which the plant biomass is digested anaerobically with at least one kind of a rumen microorganism that effects the anaerobic digestion of a plant biomass.
- at least a microorganism is a rumen microorganism and said microorganism is formed of bacteria and fungi.
- the Chinese patent document that has been filed on 21.04.2015 numbered CN104845889 of the known state of the art describes methods of the preparation, storage and application of a rumen microbial agent used for producing biogas and for efficient decomposition of cellulosic organic waste.
- the rumen content which comprises rumen bacteria has been inoculated into the anaerobic fermentation system between 10% to 50% by volume and the pH has been adjusted to 7.0-7.5 and it has been enriched and has been added to organic waste.
- the Chinese patent document that has been filed on 02.04.2009 numbered CN102459099 of the known state of the art describes a method which accelerates methane production during anaerobic digestion processes.
- glycerol-digestive microbes or pentose is added to anaerobic digesters and they substantially convert biogas into methane.
- the applications mentioned in these documents are not sufficient enough to increase methane production and hydrolysis yield at the desired amounts inside anaerobic digesters treating lignocellulosic waste.
- the need to develop the method for increasing methane yield by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats, to anaerobic digesters subject of the invention has risen.
- the aim of the invention is to carry out the method for increasing methane yield in anaerobic digesters by adding cellulolytic bacteria cocktail (cebac-g) prepared from rumen fluid of hornless goats, in order to increase the yield and hydrolysis rate of the anaerobic digester treating lignocellulose-rich waste.
- cellulolytic bacteria cocktail cebac-g
- Another aim of the invention is to carry out a method to increase methane yield by adding the cellulolytic bacteria cocktail comprising bacteria species such as Firmicutes, Clostridiaceae, Bacteroidales, Ruminococcaceae, Prevotellaceae, Lachnospiraceae, Synergi stales, Fibrobacter, Lentisphaeria, Spirochaetes and Actinobacteria to anaerobic digesters,.
- bacteria species such as Firmicutes, Clostridiaceae, Bacteroidales, Ruminococcaceae, Prevotellaceae, Lachnospiraceae, Synergi stales, Fibrobacter, Lentisphaeria, Spirochaetes and Actinobacteria to anaerobic digesters,.
- the hydrolysis rate of lignocellulose-rich waste in the anaerobic digester has been increased.
- methane production can be increased significantly, by the addition of a small amount such as 6% of the total microbial community of the digesters, of the specific cellulolytic bacteria cocktail (Firmicutes, Clostridiaceae,
- Fibrobacter, Lentisphaeria, Spirochaetes, Actinobacteria that has been developed from the rumen fluid of hornless goats, into anaerobic digesters.
- the bacteria cocktail that has been added has improved the hydrolysis phase of wheat straw which is a lignocellulolytic waste, and has increased volatile acid production, and therefore improvement in methane production has also been obtained.
- the findings that have been obtained have shown that the specific cellulolytic bacteria cocktail that has been prepared with the rumen fluid taken from hornless goats, accelerates the hydrolysis phase.
- the invention is a method for increasing methane yield by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats to anaerobic digesters, characterized in that it comprises the steps of; obtaining rumen fluid from healthy hornless goats, obtaining a specific cellulolytic bacteria cocktail by inoculation of the obtained Rumen fluid with Firmicutes, Clostridiaceae, Bacteroidales, Ruminococcaceae, Prevotellaceae, Lachnospiraceae, Synergistales,
- Fibrobacter, Lentisphaeria, Spirochaetes and Actinobacteria bacteria species adding the obtained specific cellulolytic bacteria cocktail into digesters.
- Ruminant animals such as sheep, cows, goats, llamas, etc, have the capacity to digest lignocellulosic structures of the plant material found in their diet, by means of their special digestive systems.
- the diverse microbial communities inside their stomach which is formed of four sections, play an effective role in converting the lignocellulosic structures which are difficult to digest into compounds that can be used by these animals.
- the bacterial community structure of rumen fluid is generally the same, the abundance of these communities are different in the rumen fluid.
- Cellulolytic bacteria have been enriched in the method subject to the invention, with serial culturing methods using special medium inoculated with the rumen fluid obtained from a healthy hornless goat and therefore a specific cellulolytic bacteria cocktail (cebac-g) has been prepared.
- the bacterial community of the rumen fluid obtained from hornless goats comprises 45% Bacteroidetes, 27% Spirochaetes, 19% Firmicutes, 7% Tenericutes, 1% Fibrobacters, and %1 other bacteria.
- the rumen fluid has been inoculated into the specific medium that has been developed for this method and rapid enriching studies have been carried out.
- the contents of the obtained cellulolytic bacteria cocktail are as follows: 17% Firmicutes, 12% Clostridiaceae, 11% Bacteroidales, 11% Ruminococcaceae, 8% Prevotellaceae, 7% Lachnospiraceae, 6% Synergistales, 6% Fibrobacter, 5% Lentisphaeria, 5% Spirochaetes, and 4% Actinobacteria.
- the ratios that have been provided are the volumetric ratios of the bacteria species inside biomass. According to an application of the invention, in the case that the bacteria species used have the lowest possible percentage ratio, the mixture may comprise different types of bacteria besides said bacteria.
- methane production can be increased significantly, by the addition of a small amount such as 6% of the total microbial community of the digesters, of the specific cellulolytic bacteria cocktail that has been developed by means of the rumen fluid obtained from hornless goats, into anaerobic digesters.
- the bacteria cocktail that has been added has improved the hydrolysis phase of wheat straw which is a lignocellulosic waste, and has increased volatile acid production, and therefore improvement in methane production has also been obtained.
- specific cellulolytic bacteria cocktail accelerates the hydrolysis phase.
Abstract
The invention is related to a method for increasing methane yield in anaerobic digesters, by adding a cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid of hornless goats, that has been developed in order to increase methane yield in a system by increasing the hydrolysis efficiency at a maximum level, in anaerobic digesters that are treating lignocellulosic waste, such as animal manure, agricultural waste, energy plants, food residue, sewage sludge, household wastewater and industrial wastewater. The invention is a method for increasing methane yield in anaerobic digesters by adding cellulolytic bacteria cocktail (cebac-g) prepared from rumen fluid of hornless goats, comprising the steps of obtaining the rumen fluid from hornless goats, obtaining specific cellulolytic bacteria cocktail by inoculation of the medium with bacteria such as Firmicutes, Clostridiaceae, Bacteroidales, Ruminococcaceae, Prevotellaceae, Lachnospiraceae, Synergi stales, Fibrobacter, Lentisphaeria, Spirochaetes and Actinobacteria.
Description
DESCRIPTION
METHOD FOR INCREASING METHANE YIELD IN ANAEROBIC DIGESTERS BY ADDING CELLULOLYTIC BACTERIA COCKTAIL
(CEBAC-G) PREPARED WITH RUMEN FLUID OBTAINED FROM
HORNLESS GOATS.
Technical Field
This invention is related to a method for increasing methane yield in anaerobic digesters by means of treating lignocellulosic biomass, by adding a cellulolytic bacteria cocktail (cebac-g) prepared from rumen fluid of hornless goats. More particularly, this invention is related to a method for increasing methane yield in anaerobic digesters, by adding a cellulolytic bacteria cocktail (cebac-g) prepared from rumen fluid of hornless goats (Naturally polled goats), into anaerobic digesters that has been developed in order to increase methane yield in a system by increasing the hydrolysis efficiency at a maximum level, in anaerobic digesters treating lignocellulosic biomass, such as animal manure, agricultural waste, energy plants, food residue, sewage sludge, household wastewater and industrial wastewater.
Prior Art
Agriculture and animal husbandry play an important role in the our country's economy in terms of employment opportunities created, exports, input into the industrial sector and meeting national needs. Waste created as a result of activities in the agriculture and animal husbandry sector are used as biomass in anaerobic digesters. In the near future, it is projected to use anaerobic digesters more widely within the framework of integrated biotransformation concept and waste management. Moreover large scale plants that are operated with animal waste/agri cultural waste are present. Besides this, anaerobic digesters are commonly used in Europe.
However the most important problem during the anaerobic biodegradation of these wastes which are high in lignocellulosic content is the hydrolysis phase which is considered as a rate limiting step. The recalcitrant characteristic of the biomass makes the hydrolysis of these biomasses is harder and there is a need to apply different strategies such as physical/chemical/biological pre-treatment during anaerobic digestion. The carrying out of hydrolysis with low efficiency prevents from benefiting efficiently from the biomass' energy potential of digesters which also decreases the system methane yield.
Various methods have been developed in order to increase methane yield in anaerobic digesters, in order to eliminate said problems.
The United Nations patent document filed on the date of 23.01.2009 numbered US2009137016 of the known state of the art describes a method which comprises a phase in which the plant biomass is digested anaerobically with at least one kind of a rumen microorganism that effects the anaerobic digestion of a plant biomass. According to the method at least a microorganism is a rumen microorganism and said microorganism is formed of bacteria and fungi.
The Chinese patent document that has been filed on 21.04.2015 numbered CN104845889 of the known state of the art, describes methods of the preparation, storage and application of a rumen microbial agent used for producing biogas and for efficient decomposition of cellulosic organic waste. According to the method, the rumen content which comprises rumen bacteria has been inoculated into the anaerobic fermentation system between 10% to 50% by volume and the pH has been adjusted to 7.0-7.5 and it has been enriched and has been added to organic waste. The Chinese patent document that has been filed on 02.04.2009 numbered CN102459099 of the known state of the art, describes a method which accelerates methane production during anaerobic digestion processes. According to this method, glycerol-digestive microbes or pentose is added to anaerobic digesters and they substantially convert biogas into methane.
However the applications mentioned in these documents are not sufficient enough to increase methane production and hydrolysis yield at the desired amounts inside anaerobic digesters treating lignocellulosic waste. As a result, the need to develop the method for increasing methane yield by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats, to anaerobic digesters subject of the invention has risen.
Objects and Brief Description of the Invention
The aim of the invention is to carry out the method for increasing methane yield in anaerobic digesters by adding cellulolytic bacteria cocktail (cebac-g) prepared from rumen fluid of hornless goats, in order to increase the yield and hydrolysis rate of the anaerobic digester treating lignocellulose-rich waste.
Another aim of the invention is to carry out a method to increase methane yield by adding the cellulolytic bacteria cocktail comprising bacteria species such as Firmicutes, Clostridiaceae, Bacteroidales, Ruminococcaceae, Prevotellaceae, Lachnospiraceae, Synergi stales, Fibrobacter, Lentisphaeria, Spirochaetes and Actinobacteria to anaerobic digesters,.
By means of the method subject to the invention, the hydrolysis rate of lignocellulose-rich waste in the anaerobic digester has been increased.
It has been proved that methane production can be increased significantly, by the addition of a small amount such as 6% of the total microbial community of the digesters, of the specific cellulolytic bacteria cocktail (Firmicutes, Clostridiaceae,
Bacteroidales, Ruminococcaceae, Prevotellaceae, Lachnospiraceae, Synergistales,
Fibrobacter, Lentisphaeria, Spirochaetes, Actinobacteria) that has been developed from the rumen fluid of hornless goats, into anaerobic digesters. The bacteria cocktail that has been added, has improved the hydrolysis phase of wheat straw which is a lignocellulolytic waste, and has increased volatile acid production, and therefore improvement in methane production has also been obtained. Moreover, the findings that have been obtained have shown that the specific cellulolytic
bacteria cocktail that has been prepared with the rumen fluid taken from hornless goats, accelerates the hydrolysis phase.
Detailed Description of the Invention
The invention is a method for increasing methane yield by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats to anaerobic digesters, characterized in that it comprises the steps of; obtaining rumen fluid from healthy hornless goats, obtaining a specific cellulolytic bacteria cocktail by inoculation of the obtained Rumen fluid with Firmicutes, Clostridiaceae, Bacteroidales, Ruminococcaceae, Prevotellaceae, Lachnospiraceae, Synergistales,
Fibrobacter, Lentisphaeria, Spirochaetes and Actinobacteria bacteria species adding the obtained specific cellulolytic bacteria cocktail into digesters.
Ruminant animals, such as sheep, cows, goats, llamas, etc, have the capacity to digest lignocellulosic structures of the plant material found in their diet, by means of their special digestive systems. The diverse microbial communities inside their stomach which is formed of four sections, play an effective role in converting the lignocellulosic structures which are difficult to digest into compounds that can be used by these animals. Although the bacterial community structure of rumen fluid is generally the same, the abundance of these communities are different in the rumen fluid.
Cellulolytic bacteria have been enriched in the method subject to the invention, with serial culturing methods using special medium inoculated with the rumen fluid obtained from a healthy hornless goat and therefore a specific cellulolytic bacteria cocktail (cebac-g) has been prepared.
The bacterial community of the rumen fluid obtained from hornless goats comprises 45% Bacteroidetes, 27% Spirochaetes, 19% Firmicutes, 7% Tenericutes, 1% Fibrobacters, and %1 other bacteria. The rumen fluid has been inoculated into the specific medium that has been developed for this method and rapid enriching studies have been carried out. The contents of the obtained cellulolytic bacteria cocktail (cebac-g) are as follows: 17% Firmicutes, 12% Clostridiaceae, 11% Bacteroidales, 11% Ruminococcaceae, 8% Prevotellaceae, 7% Lachnospiraceae, 6% Synergistales, 6% Fibrobacter, 5% Lentisphaeria, 5% Spirochaetes, and 4% Actinobacteria. The ratios that have been provided are the volumetric ratios of the bacteria species inside biomass. According to an application of the invention, in the case that the bacteria species used have the lowest possible percentage ratio, the mixture may comprise different types of bacteria besides said bacteria.
In order to obtain maximum performance from anaerobic digesters microorganisms that play a role during the steps of hydrolysis, acidogenesis, acetogenesis and methanogenesis need to present in the system in certain amounts and they need to function in a symbiotic relationship. The method has been planned within this scope. Besides this, when the ratios to be used were being designed, the applicability thereof in large scale systems, and the competitive relationship thereof with the present microbial community has been taken into consideration. For this reason, the specific bacteria cocktail has been added to digesters at a rate of 2%,4% or 6% of the total microbial community of the digesters. It has been determined that all three rates have a positive effect on methane yield methane production has increased by 35% within the system where 6% of specific cellulolytic bacteria cocktail.
It has been proved that methane production can be increased significantly, by the addition of a small amount such as 6% of the total microbial community of the digesters, of the specific cellulolytic bacteria cocktail that has been developed by means of the rumen fluid obtained from hornless goats, into anaerobic digesters. The bacteria cocktail that has been added, has improved the hydrolysis phase of
wheat straw which is a lignocellulosic waste, and has increased volatile acid production, and therefore improvement in methane production has also been obtained. Moreover, the findings that have been obtained have shown that specific cellulolytic bacteria cocktail accelerates the hydrolysis phase.
Claims
The invention is a method for increasing methane yield in anaerobic digesters by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats, characterized in that it comprises the steps of; obtaining rumen fluid from hornless goats, obtaining a cellulolytic bacteria cocktail by inoculation of the obtained Rumen fluid characterized by Firmicutes, Clostridiaceae, Bacteroidales, Ruminococcaceae, Prevotellaceae, Lachnospiraceae, Synergistales, Fibrobacter, Lentisphaeria, Spirochaetes and Actinobacteriabacteriaspecies in a medium. adding the obtained cellulolytic bacteria cocktail into digesters.
A method for increasing methane yield by adding cellulolytic bacteria cocktail (cebac-g) prepared from rumen fluid obtained from hornless goats to anaerobic digesters, according to claim 1, characterized in that the bacteria composition of the rumen fluid obtained from hornless goats comprises by the abundance, 45% Bacteroidetes, 27% Spirochaetes, 19% Firmicutes, 7% Tenericutes, 1% Fibrobacteres, 1% other bacteria cocktail species and in that the cellulolytic bacteria cocktail comprises by abundance at least 17% Firmicutes, at least 12% Clostridiaceae, at least 11%) Bacteroidales, at least 11% Ruminococcaceae, at least 8% Prevotellaceae, at least 7% Lachnospiraceae, at least 6% Synergistales, at least 6%Fibrobacter, at least %5 Lentisphaeria, at least 5% Spirochaetes and at least 4% Actinobacteria bacteria species.
A method for increasing methane yield by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats to anaerobic digesters, according to claim 2, characterized in that; the
cellulolytic bacteria cocktail comprises 10% different bacteria species by volume other than Firmicutes, Clostridiaceae, Bacteroidales, Ruminococcaceae, Prevotellaceae, Lachnospiraceae, Synergistales, Fibrobacter, Lentisphaeria, Spirochaetes and Actinobacteria.
A method for increasing methane yield by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats to anaerobic digesters, according to claim 1, 2 or 3, characterized in that; the cellulolytic bacteria cocktail is added to the digesters at a ratio of 2%, 4% or 6% by volume inside biomass.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/TR2017/050035 WO2018136018A1 (en) | 2017-01-20 | 2017-01-20 | Method for increasing methane yield in anaerobic digesters by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats. |
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| PCT/TR2017/050035 WO2018136018A1 (en) | 2017-01-20 | 2017-01-20 | Method for increasing methane yield in anaerobic digesters by adding cellulolytic bacteria cocktail (cebac-g) prepared with rumen fluid obtained from hornless goats. |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3808850A1 (en) | 2019-07-23 | 2021-04-21 | Recolo, SIA | A method of anaerobic digestion of food waste by novel composition of catalyst |
| WO2022102192A1 (en) * | 2020-11-13 | 2022-05-19 | 株式会社神鋼環境ソリューション | Lignocellulose decomposition system and lignocellulose decomposition method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0159054A1 (en) * | 1984-03-09 | 1985-10-23 | Stichting Katholieke Universiteit | A process for producing methane from solid vegetable material |
| US20090137016A1 (en) | 2006-12-18 | 2009-05-28 | Richard Allen Kohn | Process for rapid anaerobic digestion of biomass using microbes and the production of biofuels therefrom |
| US20110111475A1 (en) * | 2009-04-17 | 2011-05-12 | Kuhry Anthony B | Biological/Electrolytic Conversion of Biomass to Hydrocarbons |
| CN102459099A (en) | 2009-04-02 | 2012-05-16 | 新加坡科技研究局 | Methods for improving biogas production in the presence of hard substrates |
| WO2012170989A2 (en) * | 2011-06-09 | 2012-12-13 | Integrated Biochem, Llc | Process of managed ecosystem fermentation |
| CN104845889A (en) | 2015-04-21 | 2015-08-19 | 大连理工大学 | Preparation method of liquid or solid rumen functional microbial agent and application thereof |
-
2017
- 2017-01-20 WO PCT/TR2017/050035 patent/WO2018136018A1/en active Application Filing
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0159054A1 (en) * | 1984-03-09 | 1985-10-23 | Stichting Katholieke Universiteit | A process for producing methane from solid vegetable material |
| US20090137016A1 (en) | 2006-12-18 | 2009-05-28 | Richard Allen Kohn | Process for rapid anaerobic digestion of biomass using microbes and the production of biofuels therefrom |
| CN102459099A (en) | 2009-04-02 | 2012-05-16 | 新加坡科技研究局 | Methods for improving biogas production in the presence of hard substrates |
| US20110111475A1 (en) * | 2009-04-17 | 2011-05-12 | Kuhry Anthony B | Biological/Electrolytic Conversion of Biomass to Hydrocarbons |
| WO2012170989A2 (en) * | 2011-06-09 | 2012-12-13 | Integrated Biochem, Llc | Process of managed ecosystem fermentation |
| CN104845889A (en) | 2015-04-21 | 2015-08-19 | 大连理工大学 | Preparation method of liquid or solid rumen functional microbial agent and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| ISABEL S CUNHA ET AL: "andcommunity structure in the rumen microbiome of goats () from the semiarid region of Brazil", ANAEROBE, LONDON, GB, vol. 17, no. 3, 26 April 2011 (2011-04-26), pages 118 - 124, XP028239247, ISSN: 1075-9964, [retrieved on 20110507], DOI: 10.1016/J.ANAEROBE.2011.04.018 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3808850A1 (en) | 2019-07-23 | 2021-04-21 | Recolo, SIA | A method of anaerobic digestion of food waste by novel composition of catalyst |
| WO2022102192A1 (en) * | 2020-11-13 | 2022-05-19 | 株式会社神鋼環境ソリューション | Lignocellulose decomposition system and lignocellulose decomposition method |
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