WO2018119401A3 - Dispositifs et procédés pour déterminer un ou plusieurs analytes dans des fluides - Google Patents

Dispositifs et procédés pour déterminer un ou plusieurs analytes dans des fluides Download PDF

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Publication number
WO2018119401A3
WO2018119401A3 PCT/US2017/068203 US2017068203W WO2018119401A3 WO 2018119401 A3 WO2018119401 A3 WO 2018119401A3 US 2017068203 W US2017068203 W US 2017068203W WO 2018119401 A3 WO2018119401 A3 WO 2018119401A3
Authority
WO
WIPO (PCT)
Prior art keywords
devices
determining
analytes
droplet
methods
Prior art date
Application number
PCT/US2017/068203
Other languages
English (en)
Other versions
WO2018119401A2 (fr
Inventor
Marta SUAREZ FERNANDEZ
Lisa Marshall
Martina MEDKOVA
Aaron Oppenheimer
A. David Boccuti
Roberet ETHEREDGE
Eric EVJE
Frederick P. FLOYD
Mihail Lungu
Ionel Catalin POPESCU
Original Assignee
Daktari Diagnostics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daktari Diagnostics, Inc. filed Critical Daktari Diagnostics, Inc.
Publication of WO2018119401A2 publication Critical patent/WO2018119401A2/fr
Publication of WO2018119401A3 publication Critical patent/WO2018119401A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06—Investigating concentration of particle suspensions
    • G01N15/0606—Investigating concentration of particle suspensions by collecting particles on a support
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06—Investigating concentration of particle suspensions
    • G01N15/0656—Investigating concentration of particle suspensions using electric, e.g. electrostatic methods or magnetic methods
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Fluid Mechanics (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne en général des dispositifs et des procédés de détermination et/ou de quantification d'un ou de plusieurs analytes dans des fluides. Dans certains modes de réalisation, les dispositifs décrits ici peuvent être conçus de telle sorte qu'au moins deux opérations (par exemple, la lyse, la séparation magnétique, la remise en suspension, le mélange et/ou la mise à réagir) soient effectuées dans une ou plusieurs chambres de réaction. Les dispositifs et procédés décrits ici peuvent être utiles à la détermination et à la quantification de concentrations relativement faibles d'analytes présents dans un échantillon d'un patient (par ex., une gouttelette de sang total, une gouttelette de plasma, une gouttelette de fluide biologique). L'analyte peut être quantifié, dans certains cas, en liant l'analyte à une pluralité de particules contenant du métal et en mesurant le courant en changeant une tension sur une électrode active. Dans certains modes de réalisation, le dispositif est un dispositif microfluidique.
PCT/US2017/068203 2016-12-22 2017-12-22 Dispositifs et procédés pour déterminer un ou plusieurs analytes dans des fluides WO2018119401A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662438208P 2016-12-22 2016-12-22
US62/438,208 2016-12-22
US201762460673P 2017-02-17 2017-02-17
US62/460,673 2017-02-17

Publications (2)

Publication Number Publication Date
WO2018119401A2 WO2018119401A2 (fr) 2018-06-28
WO2018119401A3 true WO2018119401A3 (fr) 2019-01-03

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ID=62627205

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/068203 WO2018119401A2 (fr) 2016-12-22 2017-12-22 Dispositifs et procédés pour déterminer un ou plusieurs analytes dans des fluides

Country Status (1)

Country Link
WO (1) WO2018119401A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020091765A1 (fr) * 2018-10-31 2020-05-07 Xinova, LLC Détection de contaminants ou de particules comprenant la détection de la conductivité avec une buse de micro-injecteur
US20210394183A1 (en) * 2018-11-14 2021-12-23 Sindy Kam Yan TANG Microfluidic device and diagnostic methods for allergy testing based on detection of basophil activation
CN110079828B (zh) * 2019-05-21 2021-04-13 西安汇创贵金属新材料研究院有限公司 一种金电解废液的处理装置及处理方法
CN114401591B (zh) * 2022-01-10 2023-06-02 奥特斯科技(重庆)有限公司 用于部件承载件制造、系统和方法中的含金属流体的再生器

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7285424B2 (en) * 2002-08-27 2007-10-23 Kimberly-Clark Worldwide, Inc. Membrane-based assay devices
US20080124779A1 (en) * 2006-10-18 2008-05-29 The Regents Of The University Of California Microfluidic magnetophoretic device and methods for usig the same
US20080262213A1 (en) * 2004-05-03 2008-10-23 Betty Wu Processing Polynucleotide-Containing Samples
US20090148857A1 (en) * 2005-05-02 2009-06-11 Bioscale, Inc. Method and apparatus for detection of analyte using an acoustic device
US20100075862A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample
US20100216126A1 (en) * 2007-01-12 2010-08-26 Brunel University Microfluidic device
WO2012024658A2 (fr) * 2010-08-20 2012-02-23 IntegenX, Inc. Système d'analyse intégrée
US20140335527A1 (en) * 2013-03-15 2014-11-13 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
WO2016131574A1 (fr) * 2015-02-18 2016-08-25 Fundación Tekniker Procédé et dispositif permettant une détection et une quantification des analytes
WO2017096114A1 (fr) * 2015-12-04 2017-06-08 Daktari Diagnostics, Inc. Procédés de détermination d'analytes dans des fluides

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7285424B2 (en) * 2002-08-27 2007-10-23 Kimberly-Clark Worldwide, Inc. Membrane-based assay devices
US20080262213A1 (en) * 2004-05-03 2008-10-23 Betty Wu Processing Polynucleotide-Containing Samples
US20090148857A1 (en) * 2005-05-02 2009-06-11 Bioscale, Inc. Method and apparatus for detection of analyte using an acoustic device
US20080124779A1 (en) * 2006-10-18 2008-05-29 The Regents Of The University Of California Microfluidic magnetophoretic device and methods for usig the same
US20100216126A1 (en) * 2007-01-12 2010-08-26 Brunel University Microfluidic device
US20100075862A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample
WO2012024658A2 (fr) * 2010-08-20 2012-02-23 IntegenX, Inc. Système d'analyse intégrée
US20140335527A1 (en) * 2013-03-15 2014-11-13 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
WO2016131574A1 (fr) * 2015-02-18 2016-08-25 Fundación Tekniker Procédé et dispositif permettant une détection et une quantification des analytes
WO2017096114A1 (fr) * 2015-12-04 2017-06-08 Daktari Diagnostics, Inc. Procédés de détermination d'analytes dans des fluides

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Publication number Publication date
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