WO2018113799A8 - 构建简化基因组文库的方法及试剂盒 - Google Patents

构建简化基因组文库的方法及试剂盒 Download PDF

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Publication number
WO2018113799A8
WO2018113799A8 PCT/CN2018/074329 CN2018074329W WO2018113799A8 WO 2018113799 A8 WO2018113799 A8 WO 2018113799A8 CN 2018074329 W CN2018074329 W CN 2018074329W WO 2018113799 A8 WO2018113799 A8 WO 2018113799A8
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WIPO (PCT)
Prior art keywords
genomic library
test kit
simplified genomic
constructing
constructing simplified
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PCT/CN2018/074329
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English (en)
French (fr)
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WO2018113799A1 (zh
Inventor
王克剑
刘庆
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中国水稻研究所
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Application filed by 中国水稻研究所 filed Critical 中国水稻研究所
Priority to US16/471,584 priority Critical patent/US11912988B2/en
Publication of WO2018113799A1 publication Critical patent/WO2018113799A1/zh
Publication of WO2018113799A8 publication Critical patent/WO2018113799A8/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

提供了一种构建简化基因组文库的方法及试剂盒。该方法包括：利用第一对引物对全基因组进行非特异性扩增，得到随机扩增片段；利用第二对引物对随机扩增片段进行特异性扩增，得到简化基因组文库。
PCT/CN2018/074329 2016-12-21 2018-01-26 构建简化基因组文库的方法及试剂盒 WO2018113799A1 (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/471,584 US11912988B2 (en) 2016-12-21 2018-01-26 Method and kit for constructing a simplified genomic library

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201611193343.X 2016-12-21
CN201611193343.XA CN106676099B (zh) 2016-12-21 2016-12-21 构建简化基因组文库的方法及试剂盒

Publications (2)

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WO2018113799A1 WO2018113799A1 (zh) 2018-06-28
WO2018113799A8 true WO2018113799A8 (zh) 2019-08-01

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US (1) US11912988B2 (zh)
CN (1) CN106676099B (zh)
WO (1) WO2018113799A1 (zh)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106676099B (zh) 2016-12-21 2019-07-02 中国水稻研究所 构建简化基因组文库的方法及试剂盒
CN111690639B (zh) * 2019-03-15 2022-04-01 武汉华大智造科技有限公司 全基因组结合靶向扩增建库方法和试剂及病原检测方法
CN111763668B (zh) * 2019-04-02 2022-03-22 浙江物种链生物科技有限公司 测序引物组及基于pcr的全基因组测序方法
CN111676276A (zh) * 2020-07-13 2020-09-18 湖北伯远合成生物科技有限公司 一种快速精准确定基因编辑突变情况的方法及其应用
CN118028494B (zh) * 2024-04-15 2024-07-02 内蒙古大学 一种基于二代测序鉴定高产肉牛的引物组、方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8932812B2 (en) * 2009-12-17 2015-01-13 Keygene N.V. Restriction enzyme based whole genome sequencing
EP2859139B1 (en) * 2012-06-06 2018-08-01 The Trustees Of Princeton University Dna barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof
CN103397089B (zh) * 2013-07-24 2014-10-22 安诺优达基因科技(北京)有限公司 一种用于染色体数目异常快速检测的半特异性扩增引物组、方法及试剂盒
US10036013B2 (en) * 2013-08-19 2018-07-31 Abbott Molecular Inc. Next-generation sequencing libraries
CN104561294B (zh) * 2014-12-26 2018-03-30 北京诺禾致源科技股份有限公司 基因分型测序文库的构建方法和测序方法
CN105985945B (zh) 2015-01-30 2019-04-23 深圳华大智造科技有限公司 mRNA片段化方法及基于其构建测序文库的方法
JP6540132B2 (ja) * 2015-03-20 2019-07-10 カシオ計算機株式会社 復号装置、復号方法、及び、プログラム
CN105696088B (zh) 2015-11-11 2018-05-01 中国科学院昆明植物研究所 一种双酶切简化基因组二代测序文库构建方法及配套试剂盒
JP7343264B2 (ja) * 2016-06-29 2023-09-12 トヨタ自動車株式会社 Ｄｎａライブラリーの作製方法及びｄｎａライブラリーを用いたゲノムｄｎａ解析方法
CN106676099B (zh) * 2016-12-21 2019-07-02 中国水稻研究所 构建简化基因组文库的方法及试剂盒

Also Published As

Publication number Publication date
WO2018113799A1 (zh) 2018-06-28
CN106676099B (zh) 2019-07-02
US11912988B2 (en) 2024-02-27
CN106676099A (zh) 2017-05-17
US20200224192A1 (en) 2020-07-16

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