WO2018097593A3 - Mam-specific fluorescence calcium sensor and use thereof - Google Patents

Mam-specific fluorescence calcium sensor and use thereof Download PDF

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Publication number
WO2018097593A3
WO2018097593A3 PCT/KR2017/013340 KR2017013340W WO2018097593A3 WO 2018097593 A3 WO2018097593 A3 WO 2018097593A3 KR 2017013340 W KR2017013340 W KR 2017013340W WO 2018097593 A3 WO2018097593 A3 WO 2018097593A3
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WO
WIPO (PCT)
Prior art keywords
mam
calcium
mitochondria
calcium ion
sensor
Prior art date
Application number
PCT/KR2017/013340
Other languages
French (fr)
Korean (ko)
Other versions
WO2018097593A9 (en
WO2018097593A2 (en
Inventor
박상기
서영준
김승현
Original Assignee
포항공과대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020170154218A external-priority patent/KR102020122B1/en
Application filed by 포항공과대학교 산학협력단 filed Critical 포항공과대학교 산학협력단
Priority to US16/316,087 priority Critical patent/US11474108B2/en
Publication of WO2018097593A2 publication Critical patent/WO2018097593A2/en
Publication of WO2018097593A3 publication Critical patent/WO2018097593A3/en
Publication of WO2018097593A9 publication Critical patent/WO2018097593A9/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Inorganic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The present invention relates to a Mitochondria-Associated endoplasmic reticulum Membrane (MAM)-specific fluorescence calcium sensor and the use thereof. The present invention can surmount the limitations of a conventional technique in that verification of calcium migration through MAM requires separate measurements of calcium ion concentrations within ER and mitochondria and situational explanations of the phenomena, and can directly measure concentrations in the paths through which calcium ions move to exclude influences on calcium ion changes through numerous different calcium ion channels existing in mitochondria, thereby providing a convenient and accurate MAM-specific calcium ion sensor.
PCT/KR2017/013340 2016-11-25 2017-11-22 Mam-specific fluorescence calcium sensor and use thereof WO2018097593A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/316,087 US11474108B2 (en) 2016-11-25 2017-11-22 MAM-specific fluorescence calcium sensor and use thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20160157967 2016-11-25
KR10-2016-0157967 2016-11-25
KR1020170154218A KR102020122B1 (en) 2016-11-25 2017-11-17 MAM-specific Fluorescent Calcium Sensor and Use thereof
KR10-2017-0154218 2017-11-17

Publications (3)

Publication Number Publication Date
WO2018097593A2 WO2018097593A2 (en) 2018-05-31
WO2018097593A3 true WO2018097593A3 (en) 2018-09-27
WO2018097593A9 WO2018097593A9 (en) 2018-11-15

Family

ID=62195997

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2017/013340 WO2018097593A2 (en) 2016-11-25 2017-11-22 Mam-specific fluorescence calcium sensor and use thereof

Country Status (1)

Country Link
WO (1) WO2018097593A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050208624A1 (en) * 2001-03-05 2005-09-22 Riken Fluorescent protein

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050208624A1 (en) * 2001-03-05 2005-09-22 Riken Fluorescent protein

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ALFORD, SPENCER C.: "Dimerization-dependent green and yellow fluorescent proteins", ACS SYNTHETIC BIOLOGY, vol. 1, no. 12, 2012, pages 569 - 575, XP0002784381 *
DATABASE Nucleotide 22 June 2016 (2016-06-22), "PREDICTED: Mus musculus SAC1 suppressor of actin mutations 1-like (yea", XP055534945, retrieved from NCBI Database accession no. XM 017313703.1 *
DATABASE Nucleotide 29 April 2015 (2015-04-29), "Synthetic construct G-CaMP6 protein gene, complete cds", XP055606423, retrieved from NCBI Database accession no. KP230401.1 *
DATABASE Nucleotide 3 December 2007 (2007-12-03), "Synthetic construct Mus musculus clone IMAGE:100016169, MGC:184399 A k", XP055606401, retrieved from NCBI Database accession no. BC152925.1 *
DATABASE Nucleotide 6 October 2010 (2010-10-06), "Mus musculus adult male hippocampus cDNA, RIKEN full-length enriched l - -", XP055534964, retrieved from NCBI Database accession no. AK141611.1 *
ROWLAND, ASHLEY A.: "Endoplasmic reticulum-mitochondria contacts: function of the junction", NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2012, XP055534938 *

Also Published As

Publication number Publication date
WO2018097593A9 (en) 2018-11-15
WO2018097593A2 (en) 2018-05-31

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