WO2018097267A1 - Egg cryopreservation container - Google Patents

Egg cryopreservation container Download PDF

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Publication number
WO2018097267A1
WO2018097267A1 PCT/JP2017/042299 JP2017042299W WO2018097267A1 WO 2018097267 A1 WO2018097267 A1 WO 2018097267A1 JP 2017042299 W JP2017042299 W JP 2017042299W WO 2018097267 A1 WO2018097267 A1 WO 2018097267A1
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Prior art keywords
tag
container
main body
egg
cryopreservation
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PCT/JP2017/042299
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French (fr)
Japanese (ja)
Inventor
正成 桑山
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正成 桑山
株式会社リプロライフ
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Publication of WO2018097267A1 publication Critical patent/WO2018097267A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/42—Gynaecological or obstetrical instruments or methods
    • A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
    • A61B17/43—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for artificial insemination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/42—Gynaecological or obstetrical instruments or methods
    • A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
    • A61B17/435—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms

Definitions

  • the present invention relates to a container for cryopreserving eggs, and more specifically, a container for cryopreserving eggs such as mammalian eggs, embryos, etc., and an egg cryopreserved by interpolating an electronic tag.
  • the present invention relates to an egg cryopreservation container that facilitates identification.
  • cryopreservation of eggs such as ova and embryos of mammals is carried out by enveloping the eggs with a vitrification solution on the inner surface of a cryopreservation container such as a sterilized freezing straw.
  • a cryopreservation container such as a sterilized freezing straw.
  • a cryopreservation container since it is not easy to affix the inner surface of the container with a minimum amount of vitrification solution sufficient to encapsulate the egg, for example, the egg cryopreservation described in Patent Document 1 Tools have been devised.
  • the egg cryopreservation device described in Patent Document 1 has the advantage that it is extremely easy to collect eggs, but since the eggs are in direct contact with liquid nitrogen, the effects on the eggs have been feared.
  • an egg cryopreservation tool has been devised in which an egg cryopreservation tube containing eggs is heat-sealed and inserted into a metal protective member and then cryopreserved (see, for example, Patent Document 2).
  • An object of the present invention is to provide an egg cryopreservation container in which reading and writing from a reader / writer is facilitated by arranging an electronic tag inlet at the end of the main body of the egg cryopreservation container.
  • an egg that inserts a part of a main body from the other end of a cylindrical cap sealed at one end and seals an egg accommodated in the main body.
  • the surface of the main body to which the slip portion for attaching and holding the egg is bonded is an IC tag storage portion bonded to the end surface on the opposite side in the longitudinal direction of the main body.
  • an IC tag storage portion made of the same material as that of the main body.
  • reading / writing from a reader / writer can be facilitated by disposing the IC tag storage portion for storing the electronic tag inlet at the end of the main body of the egg cryopreservation container.
  • FIG. 1 It is a figure which shows the container for egg cryopreservation concerning one Embodiment of this invention. It is a figure which shows the method of cryopreserving the container for egg cryopreservation of this embodiment. It is a figure which shows the IC tag accommodating part of the container for egg cryopreservation of this embodiment. It is a figure which shows the detailed structure of the IC tag of this embodiment. It is a figure which shows the detailed structure of the IC tag accommodating part of this embodiment. It is a figure which shows the reading method of the IC tag of this embodiment.
  • FIG. 1 shows an egg cryopreservation container according to an embodiment of the present invention.
  • the egg cryopreservation container 1 is divided into a main body 2 and a cap 3 (FIG. 1A), and a part of the main body 2 is inserted from the other end of the cylindrical cap 3 whose one end is sealed, The egg accommodated in the main body 2 is sealed (FIG. 1 (b)).
  • the main body 2 includes a main body portion 21, a locking portion 22 that engages with the inner surface of the cap 3 when the cap 3 is inserted, and a slip portion 23 joined to the locking portion 22.
  • the slip portion 23 holds an egg attached to the tip portion thereof, and is housed on the inner surface of the cap 3 when the cap 3 is inserted. Further, an IC tag storage portion 24 in which an IC tag called an IC tag inlay or an electronic tag inlet is stored is disposed on the end surface of the main body portion 21 opposite to the surface to which the slip portion 23 is bonded. ing.
  • the outer peripheries of the cross sections of the main body 21 and the cap 3 are square, but they may be circular or other polygonal shapes.
  • the outer periphery of the cross section of the locking portion 22 and the inner periphery of the cross section of the cap 3 are circular, but any shape may be used as long as the cap 3 engages with the locking portion 22.
  • the main body portion 21 and the locking portion 22 are made of a cold-resistant resin such as polyethylene terephthalate, ABS resin, polyester, polyolefin, styrene resin, polyamide, polysulfone, fluororesin, polyimide, and are particularly resistant to the temperature of liquid nitrogen.
  • a cold-resistant resin such as polyethylene terephthalate, ABS resin, polyester, polyolefin, styrene resin, polyamide, polysulfone, fluororesin, polyimide, and are particularly resistant to the temperature of liquid nitrogen.
  • the material which has is used.
  • the slip portion 23 is a flat plate-like member having flexibility, and uses a material resistant to the temperature of liquid nitrogen in order to facilitate the egg attachment work.
  • a material resistant to the temperature of liquid nitrogen for example, synthesis of polyethylene terephthalate, 3-fluorinated polyethylene, low density polyethylene, medium density polyethylene, high density polyethylene, polycarbonate, nylon, polysulfone, polyester, polystyrene, polyimide, ultra high molecular weight polyethylene, ethylene-vinyl acetate copolymer, etc.
  • a film made of a resin or a laminate of these is used.
  • FIG. 2 shows a method of cryopreserving the egg cryopreservation container of this embodiment.
  • an egg is attached to the tip portion of the slip portion 23 and is held by vitrification liquid (FIG. 2A).
  • the cap 3 is inserted into the main body 2 in the liquid nitrogen of the liquid nitrogen tank 5, and the egg is sealed (FIG. 2 (b)).
  • a plurality of egg cryopreservation containers 1 sealed with eggs are collected and accommodated in the accommodating portion 41 from the opening 42 of the cartridge 4 (FIG. 2C).
  • the liquid in the liquid nitrogen tank 5 is gathered together so that the portion containing the egg is downward, that is, the cap 3 is downward, and the longitudinal direction of the egg cryopreservation container 1 is vertical. Immerse in nitrogen (FIG. 2 (d)).
  • the IC tag storage portion 24 of the egg cryopreservation container 1 is disposed so as to be exposed to the opening 42 of the cartridge 4.
  • FIG. 3 (a) shows the IC tag storage portion of the egg cryopreservation container of the present embodiment.
  • the IC tag storage unit 24 has a structure in which the IC tag 62 is stored in the concave portion of the concave container 61 and is sealed by the convex lid 63.
  • the concave container 61 and the convex lid 63 are made of the same material as that of the main body 21, and the concave container 61 and the convex lid 63 are welded and joined by applying a methylene chloride solution. A methylene chloride solution is also applied to the surface of the convex lid 63 opposite to the protrusion, and is welded to the main body 21 (FIG. 3B).
  • a methylene chloride solution is also applied to the surface of the convex lid 63 opposite to the protrusion, and is welded to the main body 21 (FIG. 3B).
  • FIG. 4 shows the detailed configuration of the IC tag of this embodiment.
  • the IC tag 62 is also called an IC tag inlay or an electronic tag inlet, and includes an IC chip 71 and a coiled antenna 72 connected thereto.
  • the antenna 72 is disposed on the plane of the IC chip 71, and the IC tag 62 has a cylindrical shape with a diameter of 1.8 mm and a height of 0.8 mm.
  • the communication distance between the bare IC tag 62 and the reader / writer is about 5 mm.
  • FIG. 5A shows the configuration of the concave container of the IC tag storage portion of the present embodiment.
  • the cross section of the main body 21 is a square with a side of 2.6 mm, and the joint surface of the concave container 61 with the main body 21 is also a square with a side of 2.6 mm.
  • a square having a side of 2.0 mm and a depth of 1.0 mm is formed here, and the IC tag 62 is accommodated.
  • FIG. 5B shows the configuration of the convex lid of the IC tag storage portion.
  • the joint surface of the convex lid 63 with the main body portion 21 is also a square with a side of 2.6 mm, and a convex portion with a height of 0.2 mm that fits into the concave portion of the concave container 61 is formed on the opposite surface. .
  • the four surfaces around the antenna 72 of the IC tag 62 are surrounded by the material of the concave container 61 having a thickness of 0.3 mm, and the communication distance is ensured to be 4 mm or more without impeding the transmission of radio waves. Can do.
  • FIG. 6 shows an IC tag reading method according to this embodiment.
  • the egg cryopreservation container 1 when the egg cryopreservation container 1 is taken out from the opening 42 of the cartridge 4, it is not necessary to remove all parts of the main body 2 and the cap 3 from the cartridge 4, and the IC tag storage portion 24 is opened. If the main body 2 is taken out to the extent that it comes out of the section 42, it can be read by the reader / writer 8 while being immersed in the liquid nitrogen of the liquid nitrogen tank 5.
  • the cap 3 of the egg cryopreservation container 1 is immersed in the liquid nitrogen of the liquid nitrogen tank 5 and can be read by the reader / writer 8 after taking out from the cartridge 4, without thawing the egg, Reading operation can be continued.
  • the size of the egg cryopreservation container 1 is preferably such that the cross section of the main body 21 is a square having a side of 3.0 mm or less or a circle having a diameter of 3.0 mm or less from the viewpoint of the efficiency of storage in the cartridge 4. .
  • the size of the IC tag 62 for securing a communication distance of about 5 mm is preferably set to a diameter of 2.0 mm or less based on the size of the IC tag storage portion 24 to be joined thereto. Furthermore, it is desirable that the thickness of the five surfaces around the IC tag accommodated in the IC tag accommodating portion is 1.0 mm or less.
  • a method that does not use the convex lid 63 can also be applied to store the IC tag.
  • the IC tag 62 is accommodated in the concave portion of the concave container 61 shown in FIG. 5A, PET resin heated to 200 ° C. is poured into the concave portion, the IC tag 62 is fixed, and the concave portion is filled with the resin. Thereafter, the concave container 61 and the main body portion 21 are welded and joined by ultrasonic waves.
  • the main body portion 21 and the IC tag storage portion 24 of the egg cryopreservation container 1 are separate members, and after the IC tag 62 is stored in the IC tag storage portion 24, the main body portion 21 and the IC tag storage portion 24 were joined. Alternatively, the IC tag 62 can be inserted into the main body 21 in advance.
  • the IC tag is fixed from two directions using two pins near the end surface opposite to the surface to which the slip portion 23 is joined in the longitudinal direction. After the resin is poured into the mold and solidified, the pins are removed. An IC tag housing portion is formed by pouring resin further into the hole after the pin is removed.

Abstract

The purpose of the present invention is to make it easy to read and write to and from a reader/writer by disposing an electronic tag inlet in an end section of a body of an egg cryopreservation container. The present invention is an egg cryopreservation container, in which part of a body is inserted from one end of a cylindrical cap, the other end of which is sealed, so as to hermetically seal eggs accommodated in the body, wherein the body is provided with an IC tag housing section (24) which is formed from the same material as the body and which is joined to an end surface of the body, on the side opposite in the longitudinal direction to a surface to which a slip section by which the eggs are retained by being attached thereto is joined.

Description

卵凍結保存用容器Egg cryopreservation container
　本発明は、卵凍結保存用容器に関し、より詳細には、哺乳動物の卵子、胚などの卵を凍結保存するための容器であって、電子タグを内挿することにより凍結保存された卵の識別を容易にした卵凍結保存用容器に関する。 The present invention relates to a container for cryopreserving eggs, and more specifically, a container for cryopreserving eggs such as mammalian eggs, embryos, etc., and an egg cryopreserved by interpolating an electronic tag. The present invention relates to an egg cryopreservation container that facilitates identification.
　従来、哺哺乳動物の卵子、胚などの卵の凍結保存は、滅菌処理した凍結ストローなどの凍結保存用容器の内面に、卵をガラス化液で包被して貼り付け、この凍結保存用容器を密封し、液体窒素に接触させて急速に冷却していた。このような凍結保存用容器では、卵を包被するのに充分な最少量のガラス化液で、容器の内面に貼り付ける作業が容易ではないため、例えば、特許文献１に記載の卵凍結保存用具が考案されている。 Conventionally, cryopreservation of eggs such as ova and embryos of mammals is carried out by enveloping the eggs with a vitrification solution on the inner surface of a cryopreservation container such as a sterilized freezing straw. Was sealed and rapidly cooled by contact with liquid nitrogen. In such a cryopreservation container, since it is not easy to affix the inner surface of the container with a minimum amount of vitrification solution sufficient to encapsulate the egg, for example, the egg cryopreservation described in Patent Document 1 Tools have been devised.
　特許文献１に記載の卵凍結保存用具は、卵の採取が極めて容易という利点を備えるが、卵が直接液体窒素に接触するため、卵に対する影響が危惧されていた。そこで、卵を収容した卵凍結保存用チューブをヒートシールして、金属製保護部材に挿入してから凍結保存する卵凍結保存用具が考案されている（例えば、特許文献２参照）。 The egg cryopreservation device described in Patent Document 1 has the advantage that it is extremely easy to collect eggs, but since the eggs are in direct contact with liquid nitrogen, the effects on the eggs have been feared. Thus, an egg cryopreservation tool has been devised in which an egg cryopreservation tube containing eggs is heat-sealed and inserted into a metal protective member and then cryopreserved (see, for example, Patent Document 2).
　このような卵の凍結保存においては、個体の識別を確実に行うことが必須であり、卵の取り違えは許されない。卵を収容した卵凍結保存用容器の識別は、保存用容器の側面に直接に油性インクで採取日付け、識別番号、検体名などを記載したり、同様の内容を記載したラベルまたはバーコードを印刷したラベルを、保存用容器の側面に貼り付けていた。しかしながら、径の小さな保存用容器の側面に直接に情報を記載したり、ラベルを貼り付けたりする方法では、記入誤り、貼り間違えなどの人為的なミスが生じたり、ラベルがはがれたりなど、極低温の保存環境では信頼性が低いという問題があった。そこで、ＲＦＩＤ（Radio Frequency Identification）技術を適用して、電子タグ（ＩＣタグ）を内蔵した凍結用の保存用容器が知られている（例えば、特許文献３参照）。 ¡In such cryopreservation of eggs, it is essential to reliably identify individuals, and egg confusion is not allowed. To identify the egg cryopreservation container that contains the eggs, the date of collection with oil-based ink directly on the side of the storage container, the identification number, the sample name, etc., or a label or barcode with similar contents The printed label was affixed to the side of the storage container. However, in the method of writing information directly on the side of a storage container with a small diameter or attaching a label, human error such as incorrect entry or incorrect attachment occurs, or the label is peeled off. There was a problem that reliability was low in a low temperature storage environment. Therefore, a freezing storage container incorporating an electronic tag (IC tag) by applying RFID (Radio Frequency Identification) technology is known (for example, see Patent Document 3).
特許４３７３０２５号公報Japanese Patent No. 4373025 特許４３５５１８６号公報Japanese Patent No. 4355186 特開２００７－６６０１１号公報JP 2007-66011 A
　しかしながら、特許文献３に記載されたような凍結保存用の細径管では、電子タグインレットを筒状部材に内挿するため、アンテナが、細径管の長手方向に配置されることになる。一般的に、卵凍結保存用容器は、卵が収容された部位を下方にし、保存用容器の長手方向が鉛直方向になるようにして、複数の保存用容器をまとめて液体窒素のタンクに浸漬していた。従って、リーダーライターから電子タグの情報を読み書きする場合に、保存用容器の上方から読み書きすることは困難であった。すなわち、保存用容器を液体窒素のタンクから取り出し、保存用容器の長手方向が水平になるように保持してからでないと、リーダーライターからの読み書きができないという問題があった。 However, in the small-diameter tube for cryopreservation as described in Patent Document 3, since the electronic tag inlet is inserted into the cylindrical member, the antenna is arranged in the longitudinal direction of the small-diameter tube. In general, egg cryopreservation containers are soaked in a liquid nitrogen tank, with the portion containing the eggs facing down and the longitudinal direction of the storage container being vertical. Was. Therefore, when reading / writing information of the electronic tag from the reader / writer, it is difficult to read / write from above the storage container. That is, there is a problem in that reading / writing from a reader / writer cannot be performed unless the storage container is taken out of the liquid nitrogen tank and held so that the longitudinal direction of the storage container is horizontal.
　本発明の目的は、電子タグインレットを卵凍結保存用容器の本体の端部に配置することにより、リーダーライターからの読み書きを容易にした卵凍結保存用容器を提供することにある。 An object of the present invention is to provide an egg cryopreservation container in which reading and writing from a reader / writer is facilitated by arranging an electronic tag inlet at the end of the main body of the egg cryopreservation container.
　本発明は、このような目的を達成するために、一実施態様は、一端が封止された筒状のキャップの他端から本体の一部を挿入し、本体に収容した卵を密封する卵凍結保存用容器であって、前記本体において、卵を付着させて保持するスリップ部が接合されている面とは、前記本体の長手方向に反対側の端面に接合されたＩＣタグ収納部であって、前記本体と同一の材料で形成されたＩＣタグ収納部を備えたことを特徴とする。 In order to achieve the above object, according to the present invention, in one embodiment, an egg that inserts a part of a main body from the other end of a cylindrical cap sealed at one end and seals an egg accommodated in the main body. In the main body, the surface of the main body to which the slip portion for attaching and holding the egg is bonded is an IC tag storage portion bonded to the end surface on the opposite side in the longitudinal direction of the main body. And an IC tag storage portion made of the same material as that of the main body.
　本発明によれば、電子タグインレットを収納するＩＣタグ収納部を、卵凍結保存用容器の本体の端部に配置することにより、リーダーライターからの読み書きを容易にすることができる。 According to the present invention, reading / writing from a reader / writer can be facilitated by disposing the IC tag storage portion for storing the electronic tag inlet at the end of the main body of the egg cryopreservation container.
本発明の一実施形態にかかる卵凍結保存用容器を示す図である。It is a figure which shows the container for egg cryopreservation concerning one Embodiment of this invention. 本実施形態の卵凍結保存用容器を凍結保存する方法を示す図である。It is a figure which shows the method of cryopreserving the container for egg cryopreservation of this embodiment. 本実施形態の卵凍結保存用容器のＩＣタグ収納部を示す図である。It is a figure which shows the IC tag accommodating part of the container for egg cryopreservation of this embodiment. 本実施形態のＩＣタグの詳細な構成を示す図である。It is a figure which shows the detailed structure of the IC tag of this embodiment. 本実施形態のＩＣタグ収納部の詳細な構成を示す図である。It is a figure which shows the detailed structure of the IC tag accommodating part of this embodiment. 本実施形態のＩＣタグの読み取り方法を示す図である。It is a figure which shows the reading method of the IC tag of this embodiment.
　以下、図面を参照しながら本発明の実施形態について詳細に説明する。
　図１に、本発明の一実施形態にかかる卵凍結保存用容器を示す。卵凍結保存用容器１は、本体２とキャップ３とに分かれており（図１（ａ））、一端が封止された筒状のキャップ３の他端から本体２の一部を挿入し、本体２に収容した卵を密封する（図１（ｂ））。本体２は、本体部２１と、キャップ３を挿入したときにキャップ３の内面と係合する係止部２２と、係止部２２に接合されたスリップ部２３とを備える。スリップ部２３は、その先端部分に卵を付着させて保持し、キャップ３を挿入したときにキャップ３の内面に収納される。さらに、スリップ部２３が接合されている面とは長手方向に反対側の本体部２１の端面には、ＩＣタグインレイまたは電子タグインレットとも呼ばれるＩＣタグが収納されたＩＣタグ収納部２４が配置されている。 Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.
FIG. 1 shows an egg cryopreservation container according to an embodiment of the present invention. The egg cryopreservation container 1 is divided into a main body 2 and a cap 3 (FIG. 1A), and a part of the main body 2 is inserted from the other end of the cylindrical cap 3 whose one end is sealed, The egg accommodated in the main body 2 is sealed (FIG. 1 (b)). The main body 2 includes a main body portion 21, a locking portion 22 that engages with the inner surface of the cap 3 when the cap 3 is inserted, and a slip portion 23 joined to the locking portion 22. The slip portion 23 holds an egg attached to the tip portion thereof, and is housed on the inner surface of the cap 3 when the cap 3 is inserted. Further, an IC tag storage portion 24 in which an IC tag called an IC tag inlay or an electronic tag inlet is stored is disposed on the end surface of the main body portion 21 opposite to the surface to which the slip portion 23 is bonded. ing.
　本実施形態では、本体部２１とキャップ３の断面の外周は方形であるが、円形であっても、その他の多角形の形状であっても良い。係止部２２の断面の外周とキャップ３の断面の内周は円形であるが、キャップ３が係止部２２に係合するのであれば、どのような形状でもよい。 In this embodiment, the outer peripheries of the cross sections of the main body 21 and the cap 3 are square, but they may be circular or other polygonal shapes. The outer periphery of the cross section of the locking portion 22 and the inner periphery of the cross section of the cap 3 are circular, but any shape may be used as long as the cap 3 engages with the locking portion 22.
　本体部２１と係止部２２とは、ポリエチレンテレフタレート、ＡＢＳ樹脂、ポリエステル、ポリオレフィン、スチレン系樹脂、ポリアミド、ポリサルホン、フッ素樹脂、ポリイミド等の耐寒性樹脂からなり、特に、液体窒素の温度に耐性を有する材料を用いる。 The main body portion 21 and the locking portion 22 are made of a cold-resistant resin such as polyethylene terephthalate, ABS resin, polyester, polyolefin, styrene resin, polyamide, polysulfone, fluororesin, polyimide, and are particularly resistant to the temperature of liquid nitrogen. The material which has is used.
　スリップ部２３は、卵の付着作業を容易にするために、可撓性を有する平坦な板状の部材であって、液体窒素の温度に耐性を有する材料を用いる。例えば、ポリエチレンテレフタレート、３－フッ化ポリエチレン、低密度ポリエチレン、中密度ポリエチレン、高密度ポリエチレン、ポリカーボネート、ナイロン、ポリスルホン、ポリエステル、ポリスチレン、ポリイミド、超高分子量ポリエチレン、エチレン－酢酸ビニル共重合体などの合成樹脂からなるフィルム、またはこれらの積層体が用いられる。 The slip portion 23 is a flat plate-like member having flexibility, and uses a material resistant to the temperature of liquid nitrogen in order to facilitate the egg attachment work. For example, synthesis of polyethylene terephthalate, 3-fluorinated polyethylene, low density polyethylene, medium density polyethylene, high density polyethylene, polycarbonate, nylon, polysulfone, polyester, polystyrene, polyimide, ultra high molecular weight polyethylene, ethylene-vinyl acetate copolymer, etc. A film made of a resin or a laminate of these is used.
　図２に、本実施形態の卵凍結保存用容器を凍結保存する方法を示す。最初に、スリップ部２３の先端部分に卵を付着させてガラス化液により保持する（図２（ａ））。液体窒タンク５の液体窒素中で本体２にキャップ３を挿入し、卵を密封する（図２（ｂ））。同様に卵を密封した複数の卵凍結保存用容器１をまとめて、カートリッジ４の開口部４２から収納部４１に収容する（図２（ｃ））。卵が収容された部位を下方にし、すなわちキャップ３を下方にして、卵凍結保存用容器１の長手方向が鉛直方向になるようにして、カートリッジ４を複数個まとめて液体窒素タンク５内の液体窒素に浸漬する（図２（ｄ））。 FIG. 2 shows a method of cryopreserving the egg cryopreservation container of this embodiment. First, an egg is attached to the tip portion of the slip portion 23 and is held by vitrification liquid (FIG. 2A). The cap 3 is inserted into the main body 2 in the liquid nitrogen of the liquid nitrogen tank 5, and the egg is sealed (FIG. 2 (b)). Similarly, a plurality of egg cryopreservation containers 1 sealed with eggs are collected and accommodated in the accommodating portion 41 from the opening 42 of the cartridge 4 (FIG. 2C). The liquid in the liquid nitrogen tank 5 is gathered together so that the portion containing the egg is downward, that is, the cap 3 is downward, and the longitudinal direction of the egg cryopreservation container 1 is vertical. Immerse in nitrogen (FIG. 2 (d)).
　このような構成により、卵凍結保存用容器１のＩＣタグ収納部２４は、カートリッジ４の開口部４２に露出するように配置される。 With such a configuration, the IC tag storage portion 24 of the egg cryopreservation container 1 is disposed so as to be exposed to the opening 42 of the cartridge 4.
　図３（ａ）に、本実施形態の卵凍結保存用容器のＩＣタグ収納部を示す。ＩＣタグ収納部２４は、凹状容器６１の凹部にＩＣタグ６２を収容し、凸状蓋６３により密封された構造を有する。 FIG. 3 (a) shows the IC tag storage portion of the egg cryopreservation container of the present embodiment. The IC tag storage unit 24 has a structure in which the IC tag 62 is stored in the concave portion of the concave container 61 and is sealed by the convex lid 63.
　凹状容器６１と凸状蓋６３とは、本体部２１と同じ材料を用い、凹状容器６１と凸状蓋６３とは、メチレンクロライド溶液を塗布して溶着接合する。凸状蓋６３の突起部の反対の面にもメチレンクロライド溶液を塗布して、本体部２１と溶着接合する（図３（ｂ））。ＩＣタグ収納部２４と本体部２１のいずれも同じ材料を用いることにより、極低温下における材料の収縮率の違いから生じる破損、亀裂、剥がれ落ちなどを防ぐことができる。なお、凹状容器６１と凸状蓋６３との接合、ＩＣタグ収納部２４と本体部２１との接合は、超音波の照射によって接合することもできる。 The concave container 61 and the convex lid 63 are made of the same material as that of the main body 21, and the concave container 61 and the convex lid 63 are welded and joined by applying a methylene chloride solution. A methylene chloride solution is also applied to the surface of the convex lid 63 opposite to the protrusion, and is welded to the main body 21 (FIG. 3B). By using the same material for both the IC tag storage portion 24 and the main body portion 21, it is possible to prevent breakage, cracks, peeling off, and the like resulting from differences in material shrinkage at extremely low temperatures. The bonding between the concave container 61 and the convex lid 63 and the bonding between the IC tag storage unit 24 and the main body unit 21 can also be performed by irradiation with ultrasonic waves.
　図４に、本実施形態のＩＣタグの詳細な構成を示す。ＩＣタグ６２は、ＩＣタグインレイまたは電子タグインレットとも呼ばれ、ＩＣチップ７１と、これに接続されたコイル状のアンテナ７２とから構成されている。アンテナ７２は、ＩＣチップ７１の平面上に配置され、ＩＣタグ６２は、直径１．８ｍｍ、高さ０．８ｍｍの筒状の形状を有する。 FIG. 4 shows the detailed configuration of the IC tag of this embodiment. The IC tag 62 is also called an IC tag inlay or an electronic tag inlet, and includes an IC chip 71 and a coiled antenna 72 connected thereto. The antenna 72 is disposed on the plane of the IC chip 71, and the IC tag 62 has a cylindrical shape with a diameter of 1.8 mm and a height of 0.8 mm.
　ＨＦ帯（１３．５６ＭＨｚ）の電波を使用する場合、裸のＩＣタグ６２とリーダーライターとの通信距離は５ｍｍ程度である。 When using radio waves in the HF band (13.56 MHz), the communication distance between the bare IC tag 62 and the reader / writer is about 5 mm.
　図５（ａ）に、本実施形態のＩＣタグ収納部の凹状容器の構成を示す。本体部２１の断面は、一辺２．６ｍｍの正方形であり、凹状容器６１の本体部２１との接合面も、一辺２．６ｍｍの正方形である。ここに一辺２．０ｍｍの正方形で深さ１．０ｍｍの凹部が形成され、ＩＣタグ６２が収容される。 FIG. 5A shows the configuration of the concave container of the IC tag storage portion of the present embodiment. The cross section of the main body 21 is a square with a side of 2.6 mm, and the joint surface of the concave container 61 with the main body 21 is also a square with a side of 2.6 mm. A square having a side of 2.0 mm and a depth of 1.0 mm is formed here, and the IC tag 62 is accommodated.
　図５（ｂ）に、ＩＣタグ収納部の凸状蓋の構成を示す。凸状蓋６３の本体部２１との接合面も、一辺２．６ｍｍの正方形であり、反対の面には、凹状容器６１の凹部に勘合する高さ０．２ｍｍの凸部が形成されている。 FIG. 5B shows the configuration of the convex lid of the IC tag storage portion. The joint surface of the convex lid 63 with the main body portion 21 is also a square with a side of 2.6 mm, and a convex portion with a height of 0.2 mm that fits into the concave portion of the concave container 61 is formed on the opposite surface. .
　このような構成により、ＩＣタグ６２のアンテナ７２の周囲の４つの面は、厚さ０．３ｍｍの凹状容器６１の材料に取り囲まれ、電波の透過を妨げず、通信距離を４ｍｍ以上確保することができる。 With such a configuration, the four surfaces around the antenna 72 of the IC tag 62 are surrounded by the material of the concave container 61 having a thickness of 0.3 mm, and the communication distance is ensured to be 4 mm or more without impeding the transmission of radio waves. Can do.
　図６に、本実施形態のＩＣタグの読み取り方法を示す。本実施形態によれば、カートリッジ４の開口部４２から卵凍結保存用容器１を取り出す際に、本体２とキャップ３の全ての部分をカートリッジ４から取り出す必要は無く、ＩＣタグ収納部２４が開口部４２から出る程度に、本体２を取り出せば、液体窒タンク５の液体窒素に浸漬したまま、リーダーライター８による読み取りが可能となる。 FIG. 6 shows an IC tag reading method according to this embodiment. According to this embodiment, when the egg cryopreservation container 1 is taken out from the opening 42 of the cartridge 4, it is not necessary to remove all parts of the main body 2 and the cap 3 from the cartridge 4, and the IC tag storage portion 24 is opened. If the main body 2 is taken out to the extent that it comes out of the section 42, it can be read by the reader / writer 8 while being immersed in the liquid nitrogen of the liquid nitrogen tank 5.
　また、カートリッジ４から取り出した後、卵凍結保存用容器１のキャップ３部を液体窒タンク５の液体窒素に浸漬したまま、リーダーライター８による読み取りが可能となるので、卵を解凍させることなく、読み取り作業を継続することができる。 In addition, since the cap 3 of the egg cryopreservation container 1 is immersed in the liquid nitrogen of the liquid nitrogen tank 5 and can be read by the reader / writer 8 after taking out from the cartridge 4, without thawing the egg, Reading operation can be continued.
　なお、卵凍結保存用容器１の大きさは、本体部２１の断面は、カートリッジ４への収納効率の観点から、一辺３．０ｍｍ以下の正方形、または直径３．０ｍｍ以下の円形が好適である。これに接合するＩＣタグ収納部２４の大きさから、通信距離を５ｍｍ程度確保するためのＩＣタグ６２の大きさは、直径２．０ｍｍ以下とするのが好ましい。さらに、前記ＩＣタグ収納部は、その内部に収納されたＩＣタグの周囲の５つの面の厚さが１．０ｍｍ以下であることが望ましい。 The size of the egg cryopreservation container 1 is preferably such that the cross section of the main body 21 is a square having a side of 3.0 mm or less or a circle having a diameter of 3.0 mm or less from the viewpoint of the efficiency of storage in the cartridge 4. . The size of the IC tag 62 for securing a communication distance of about 5 mm is preferably set to a diameter of 2.0 mm or less based on the size of the IC tag storage portion 24 to be joined thereto. Furthermore, it is desirable that the thickness of the five surfaces around the IC tag accommodated in the IC tag accommodating portion is 1.0 mm or less.
　ＩＣタグの収納には、凸状蓋６３を用いない方法も適用できる。図５（ａ）に示した凹状容器６１の凹部にＩＣタグ６２を収容し、２００℃に加熱したＰＥＴ樹脂を凹部に流し込み、ＩＣタグ６２を固定するとともに、凹部を樹脂で充填してしまう。その後、凹状容器６１と本体部２１とを、超音波により溶着させて接合する。 A method that does not use the convex lid 63 can also be applied to store the IC tag. The IC tag 62 is accommodated in the concave portion of the concave container 61 shown in FIG. 5A, PET resin heated to 200 ° C. is poured into the concave portion, the IC tag 62 is fixed, and the concave portion is filled with the resin. Thereafter, the concave container 61 and the main body portion 21 are welded and joined by ultrasonic waves.
　実施例１，２において、卵凍結保存用容器１の本体部２１とＩＣタグ収納部２４とは、それぞれ別個の部材であり、ＩＣタグ収納部２４にＩＣタグ６２を収容してから、本体部２１とＩＣタグ収納部２４と接合していた。代替的に、予め本体部２１にＩＣタグ６２を内挿することもできる。 In Examples 1 and 2, the main body portion 21 and the IC tag storage portion 24 of the egg cryopreservation container 1 are separate members, and after the IC tag 62 is stored in the IC tag storage portion 24, the main body portion 21 and the IC tag storage portion 24 were joined. Alternatively, the IC tag 62 can be inserted into the main body 21 in advance.
　本体部２１の金型において、スリップ部２３が接合されている面とは長手方向に反対側の端面付近に、２つのピンを用いて、ＩＣタグを二方向から固定しておく。この金型に樹脂を流し込み、固化した後に、ピンを除去する。ピンを除去した後の穴に、さらに樹脂を流し込むことによって、ＩＣタグ収納部を形成する。 In the mold of the main body 21, the IC tag is fixed from two directions using two pins near the end surface opposite to the surface to which the slip portion 23 is joined in the longitudinal direction. After the resin is poured into the mold and solidified, the pins are removed. An IC tag housing portion is formed by pouring resin further into the hole after the pin is removed.
　１　卵凍結保存用容器
　２　本体
　３　キャップ
　２１　本体部
　２２　係止部
　２３　スリップ部
　２４　ＩＣタグ収納部
　６１　凹状容器
　６２　ＩＣタグ
　６３　凸状蓋
　７１　ＩＣチップ
　７２　アンテナ DESCRIPTION OF SYMBOLS 1 Egg cryopreservation container 2 Main body 3 Cap 21 Main body part 22 Locking part 23 Slip part 24 IC tag storage part 61 Concave container 62 IC tag 63 Convex lid 71 IC chip 72 Antenna

Claims (6)

  1. 　一端が封止された筒状のキャップの他端から本体の一部を挿入し、本体に収容した卵を密封する卵凍結保存用容器であって、
    　前記本体において、卵を付着させて保持するスリップ部が接合されている面とは、前記本体の長手方向に反対側の端面に接合されたＩＣタグ収納部であって、前記本体と同一の材料で形成されたＩＣタグ収納部を備えたことを特徴とする卵凍結保存用容器。     Inserting a part of the main body from the other end of the cylindrical cap whose one end is sealed, an egg cryopreservation container that seals the eggs contained in the main body,
    In the main body, the surface to which the slip portion for attaching and holding the egg is bonded is an IC tag storage portion bonded to an end surface on the opposite side in the longitudinal direction of the main body, and the same material as the main body A container for cryopreservation of eggs, comprising an IC tag storage portion formed in the above.
  2. 　前記ＩＣタグ収納部は、その内部に収納されたＩＣタグの周囲の５つの面の厚さが１．０ｍｍ以下であることを特徴とする請求項１に記載の卵凍結保存用容器。 The container for cryopreservation of eggs according to claim 1, wherein the IC tag storage unit has a thickness of five surfaces around the IC tag stored therein that is 1.0 mm or less.
  3. 　前記ＩＣタグの前記長手方向と垂直な断面は、直径が２ｍｍ以下であることを特徴とする請求項２に記載の卵凍結保存用容器。 The egg cryopreservation container according to claim 2, wherein the cross section perpendicular to the longitudinal direction of the IC tag has a diameter of 2 mm or less.
  4. 　前記ＩＣタグ収納部は、ＩＣタグを収容する凹部が形成された凹状容器と、該凹状容器の凹部に勘合する凸部が形成された凸状蓋とを含むことを特徴とする請求項１ないし３のいずれかに記載の卵凍結保存用容器。 The said IC tag accommodating part contains the concave container in which the recessed part which accommodates an IC tag was formed, and the convex cover in which the convex part which fits into the recessed part of this concave container was formed. The container for egg cryopreservation according to any one of 3 above.
  5. 　前記ＩＣタグ収納部は、凹部が形成された凹状容器にＩＣタグを収容し、前記凹部に前記本体と同一の材料が充填されていることを特徴とする請求項１ないし３のいずれかに記載の卵凍結保存用容器。 The said IC tag accommodating part accommodates an IC tag in the concave container in which the recessed part was formed, The same material as the said main body is filled into the said recessed part, The Claim 1 thru | or 3 characterized by the above-mentioned. Egg cryopreservation container.
  6. 　前記ＩＣタグ収納部は、前記反対側の端面にＩＣタグが予め内挿された部分であることを特徴とする請求項１ないし３のいずれかに記載の卵凍結保存用容器。 4. The egg cryopreservation container according to claim 1, wherein the IC tag storage portion is a portion in which an IC tag is inserted in advance on the opposite end surface.
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