WO2018076226A1

WO2018076226A1 - Multi-color laser modulation method and system

Info

Publication number: WO2018076226A1
Application number: PCT/CN2016/103516
Authority: WO
Inventors: 贾宏博; 周镇乔
Original assignee: 中国科学院苏州生物医学工程技术研究所
Priority date: 2016-10-27
Filing date: 2016-10-27
Publication date: 2018-05-03

Abstract

A multi-color laser modulation system, comprising: a multi-color laser device (1), a radio frequency signal generator (3), a frequency mixer (4), and an acousto-optic modulator (2); the radio frequency signal generator (3) generates at least two radio frequency signals having different frequencies to the frequency mixer (4), the frequency mixer (4) synthesizing the at least two radio frequency signals having different frequencies into one driving radio frequency signal; the multi-color laser device (1) generates at least two lasers having different wavelengths, the driving radio frequency signal generated by the frequency mixer (4) driving the acousto-optic modulator (2) to simultaneously modulate the at least two lasers having different wavelengths generated by the multi-color laser device (1).

Description

Modulation method and system for multicolor laser

Technical field

The present application relates to a method and system for modulating a multicolor laser.

Background technique

With the development of fluorescent labeling technology, fluorescent probes of various colors have been developed. Currently, biologists are increasingly inclined to use multiple fluorescent probes to label multiple molecules or cell types in the same sample. However, the excitation spectra of fluorescent probes of various colors are also different, that is, the wavelengths of the required femtosecond lasers are different, and the intensity of light required for excitation is also different. Therefore, it is necessary to simultaneously perform excitation imaging of a multi-wavelength femtosecond laser.

The most commonly used Ti:Sapphire femtosecond lasers, although continuously adjustable in wavelength, can only output one wavelength at a time. With the development of laser technology, fiber lasers that simultaneously emit multi-color femtosecond lasers have recently emerged, but there is still a need for a method of simultaneously modulating a multi-color femtosecond laser.

Summary of the invention

In view of this, it is necessary to provide a modulation method and system for a multicolor laser that can simultaneously modulate a plurality of different wavelength color lasers.

In order to solve the above technical problem, the present application provides a modulation system for a multi-color laser, including a multi-color laser, a radio frequency signal generator, a mixer, and an acousto-optic modulator, the radio frequency signal generator generating at least different frequencies. Transmitting two RF signals to the mixer, the mixer combining at least two radio frequency signals of the different frequencies into one driving radio frequency signal, the multi-color laser generating at least two lasers of different wavelengths, the mixing The driving RF signal generated by the frequency converter drives the acousto-optic modulator to simultaneously modulate the laser light of at least two different wavelengths generated by the multi-color laser.

Preferably, an output of the radio frequency signal generator is coupled to an input of the mixer, and an output of the polychromatic laser and the mixer is coupled to an input of the acousto-optic modulator.

Preferably, the laser is a femtosecond laser.

Preferably, the driving radio frequency signal contains a number of frequencies corresponding to the number of lasers of the different wavelengths.

Preferably, the plurality of femtosecond lasers of different wavelengths are modulated by the acousto-optic modulator to output a plurality of lasers of different wavelengths and adjustable light intensity.

Preferably, the intensity of the multi-color laser output by the acousto-optic modulator is adjusted by controlling the power of the radio frequency signal of the corresponding frequency.

To solve the above problems, the present application also provides a method for modulating a multicolor laser, comprising:

Combining multiple RF signals of different frequencies into one driving RF signal containing a plurality of different frequencies;

A plurality of femtosecond lasers having different wavelengths are modulated by the driving RF signal.

Preferably, the plurality of radio frequency signals of different frequencies are combined into a driving RF signal containing a plurality of different frequencies, including:

After the at least two radio frequency signals of different frequencies are passed through the electronic device, at least the radio frequency signals of different frequencies are combined into a driving RF signal, and the synthesized driving RF signal contains the frequency of the plurality of RF signals before synthesis.

Preferably, the driving the radio frequency signal is used to modulate a plurality of femtosecond lasers having different wavelengths, including:

The different wavelengths of the femtosecond laser are separately modulated by different frequency components in the driving RF signal; the frequency in the driving RF signal is in one-to-one correspondence with the different wavelengths of femtosecond laser.

Preferably, the plurality of femtosecond lasers of different wavelengths after modulation adjust the light intensity by controlling the power of the radio frequency signal of the corresponding frequency.

The present application synthesizes a plurality of different frequency radio frequency signals into a driving RF signal containing a plurality of different frequencies, and then drives the driving RF signal to drive the acousto-optic modulator to make the acousto-optic modulator simultaneously modulated. A femtosecond laser of different wavelengths makes the intensity of the femtosecond laser of each wavelength independently adjustable, which solves the problem that only one wavelength of the laser can be simultaneously modulated in the prior art.

DRAWINGS

The drawings described herein are provided to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and the description thereof are for explaining the present application and do not constitute an undue limitation of the present application. In the drawing:

FIG. 1 is a schematic diagram of a modulation system of a multicolor laser of the present application.

detailed description

The technical solutions of the present application will be clearly and completely described in the following with reference to the specific embodiments of the present application and the corresponding drawings. It is apparent that the described embodiments are only a part of the embodiments of the present application, and not all of them. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments of the present application without departing from the inventive scope are the scope of the present application.

The technical solutions provided by the embodiments of the present application are described in detail below with reference to the accompanying drawings.

Referring to FIG. 1, the modulation system of the multicolor laser of the present application includes a multicolor laser 1, a radio frequency signal generator 3, a mixer 4, and an acousto-optic modulator 2. An output of the RF signal generator 3 is coupled to an input of the mixer 4, and an output of the polychromatic laser 1 and the mixer 4 is coupled to an input of the acousto-optic modulator 2.

The multi-color laser 1 generates a plurality of femtosecond lasers of wavelength (λ1, λ2, λ3 in the drawing) into the acousto-optic modulator 2. The RF signal generator 3 generates a plurality of initial RF signals having different frequencies (such as f1, f2, and f3 in the figure), and the plurality of initial RF signals are combined with the mixer 4 to synthesize a driving RF signal, and the driving RF The signal contains multiple frequency components, ie the frequency components of multiple initial RF signals.

The driving RF signal drives the acousto-optic modulator 2 to modulate a plurality of femtosecond lasers having different wavelengths, and lasers of each of a plurality of femtosecond lasers (ie, λ1 in the figure) The light intensities of the three wavelengths of λ2 and λ3 are independently adjustable.

The acousto-optic modulator 2 is an optical device that modulates a laser using ultrasonic vibration, which generates ultrasonic vibrations in an acousto-optic crystal by a radio frequency drive signal, thereby modulating a laser light incident on the acousto-optic crystal. When the acousto-optic modulator 2 modulates lasers of different wavelengths, RF driving of different frequencies is required. The signal, the laser wavelength and the RF signal frequency are in one-to-one correspondence. When it is required to simultaneously modulate a multi-color laser, the radio frequency signal required by the acousto-optic device must contain a plurality of frequency components.

The multicolor laser modulation method of the present application includes the following steps:

S101. Synthesize a plurality of radio frequency signals of different frequencies into a driving RF signal that includes multiple frequencies.

In this step, after at least two radio frequency signals of different frequencies are passed through a certain electronic device, at least two radio frequency signals of different frequencies are combined into one driving radio frequency signal, and the synthesized driving radio frequency signal includes a plurality of pre-synthesis radio frequency signals. Frequency of.

S102. Modulate a multi-color femtosecond laser having different wavelengths by using the driving RF signal.

In this step, the different wavelength components in the driving RF signal are respectively used to modulate the different wavelengths of the femtosecond laser, and the frequency in the driving RF signal is in one-to-one correspondence with the femtosecond lasers of different wavelengths.

The present application synthesizes a plurality of radio frequency signals of different frequencies into a driving RF signal containing a plurality of different frequencies, and then drives the driving RF signal to drive the acousto-optic modulator 2, so that the acousto-optic modulator 2 simultaneously The modulation of a plurality of femtosecond lasers of different wavelengths makes the intensity of the femtosecond laser of each wavelength independently adjustable, and solves the problem that only one wavelength of the laser can be simultaneously modulated in the prior art.

Those skilled in the art will appreciate that embodiments of the present invention can be provided as a method, system, or computer program product. Accordingly, the present invention may take the form of an entirely hardware embodiment, an entirely software embodiment, or a combination of software and hardware. Moreover, the invention can take the form of a computer program product embodied on one or more computer-usable storage media (including but not limited to disk storage, CD-ROM, optical storage, etc.) including computer usable program code.

It is also to be understood that the terms "comprises" or "comprising" or "comprising" or any other variations are intended to encompass a non-exclusive inclusion, such that a process, method, article, Other elements not explicitly listed, or elements that are inherent to such a process, method, commodity, or equipment. In the absence of more restrictions, elements defined by the phrase "including one..." are not excluded from the process, method, article, or design that includes the element. There are other identical elements in the preparation.

The above description is only an embodiment of the present application and is not intended to limit the application. Various changes and modifications can be made to the present application by those skilled in the art. Any modifications, equivalents, improvements, etc. made within the spirit and scope of the present application are intended to be included within the scope of the appended claims.

Claims

A multicolor laser modulation system, comprising: a multicolor laser, a radio frequency signal generator, a mixer, and an acousto-optic modulator, wherein the radio frequency signal generator generates at least two radio frequency signals of different frequencies to the a mixer that combines at least two radio frequency signals of the different frequencies into one driving radio frequency signal, the multi-color laser generates laser light of at least two different wavelengths, and the driving RF generated by the mixer The signal drives the acousto-optic modulator to simultaneously modulate laser light of at least two different wavelengths produced by the multi-color laser.
A modulation system for a multicolor laser according to claim 1, wherein an output of said radio frequency signal generator is coupled to an input of said mixer, said multicolor laser and an output of said mixer The terminals are each connected to an input of the acousto-optic modulator.
A multicolor laser modulation system according to claim 1, wherein said laser light is a femtosecond laser.
A multicolor laser modulation system according to claim 1, wherein said driving radio frequency signal has a frequency number corresponding to the number of lasers of said different wavelengths.
The modulation system of a multicolor laser according to claim 1, wherein the plurality of femtosecond lasers of different wavelengths are modulated by the acousto-optic modulator to output a plurality of lasers of different wavelengths and adjustable light intensity. .
The modulation system of a multicolor laser according to claim 5, wherein the intensity of the multicolor laser output by the acousto-optic modulator is adjusted by controlling the power of the radio frequency signal of the corresponding frequency.
A method for modulating a multicolor laser, comprising:

Combining multiple RF signals of different frequencies into one driving RF signal containing a plurality of different frequencies;

A plurality of femtosecond lasers having different wavelengths are modulated by the driving RF signal.
The method according to claim 7, wherein the plurality of radio frequency signals of different frequencies are combined into a driving RF signal having a plurality of different frequencies, including:

After passing at least two radio frequency signals of different frequencies through the action of the electronic device, different frequencies are The less RF signal synthesizes a driving RF signal, and the synthesized driving RF signal contains the frequencies of the plurality of RF signals before synthesis.
The method of claim 7 wherein modulating a plurality of femtosecond lasers having different wavelengths using said driving RF signal comprises:

The different wavelengths of the femtosecond laser are separately modulated by different frequency components in the driving RF signal; the frequency in the driving RF signal is in one-to-one correspondence with the different wavelengths of femtosecond laser.
The method of claim 9 wherein said plurality of femtosecond lasers of different wavelengths are modulated to adjust the intensity of light by controlling the power of said corresponding frequency of the radio frequency signal.