WO2018041726A1 - Biomarqueurs de détermination de la présence d'une plaque d'athérome instable - Google Patents

Biomarqueurs de détermination de la présence d'une plaque d'athérome instable Download PDF

Info

Publication number
WO2018041726A1
WO2018041726A1 PCT/EP2017/071394 EP2017071394W WO2018041726A1 WO 2018041726 A1 WO2018041726 A1 WO 2018041726A1 EP 2017071394 W EP2017071394 W EP 2017071394W WO 2018041726 A1 WO2018041726 A1 WO 2018041726A1
Authority
WO
WIPO (PCT)
Prior art keywords
mir
determining
mirna
atherosclerotic plaque
atherosclerosis
Prior art date
Application number
PCT/EP2017/071394
Other languages
English (en)
Inventor
Sara Johanna Pinto-Sietsma
Esther Elisa Johanna Maria Creemers
Josephus Cornelis Maria Meijers
Maurice Wilhelmus Jacobus DE RONDE
Maayke Guda Maria KOK
Original Assignee
Academisch Medisch Centrum
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Academisch Medisch Centrum filed Critical Academisch Medisch Centrum
Publication of WO2018041726A1 publication Critical patent/WO2018041726A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Abstract

L'invention concerne un procédé permettant de déterminer la présence d'une plaque d'athérome instable chez un sujet, ledit procédé comprenant les étapes consistant à déterminer le taux d'expression d'un ou de plusieurs miARN choisis dans le groupe constitué par miR-223-3p, miR-125b-5p, miR-142-3p et miR-193b-3p dans un échantillon biologique prélevé chez ledit sujet ; comparer ledit taux d'expression avec un taux de référence, et déterminer la présence ou non de ladite plaque d'athérome instable en fonction desdites informations. Le même miARN et miR-499a-5p peuvent être utilisés pour diagnostiquer chez le sujet le risque de souffrir d'athérosclérose ou de développer l'athérosclérose.
PCT/EP2017/071394 2016-08-27 2017-08-25 Biomarqueurs de détermination de la présence d'une plaque d'athérome instable WO2018041726A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP16186061.4 2016-08-27
EP16186061 2016-08-27

Publications (1)

Publication Number Publication Date
WO2018041726A1 true WO2018041726A1 (fr) 2018-03-08

Family

ID=56842691

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2017/071394 WO2018041726A1 (fr) 2016-08-27 2017-08-25 Biomarqueurs de détermination de la présence d'une plaque d'athérome instable

Country Status (1)

Country Link
WO (1) WO2018041726A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021153753A1 (fr) * 2020-01-30 2021-08-05 東レ株式会社 Procédé, dispositif et programme d'examen

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008153692A2 (fr) 2007-05-22 2008-12-18 The Brigham And Women's Hospital, Inc. Établissement de profil d'expression de micro arn du fluide cérébrospinal
WO2010135570A1 (fr) * 2009-05-20 2010-11-25 Board Of Regents, The University Of Texas System Identification de micro-arn impliqués dans un remodelage post-infarctus du myocarde et une insuffisance cardiaque
WO2010139810A1 (fr) 2009-06-05 2010-12-09 Febit Holding Gmbh Empreinte de micro-arn utilisée dans le diagnostic du cancer du poumon
WO2011163214A2 (fr) 2010-06-21 2011-12-29 Diogenix, Inc. Profils de micro-arn pour une évaluation de la sclérose en plaques
WO2015177368A1 (fr) * 2014-05-22 2015-11-26 Servicio Andaluz De Salud Procédé pour faire circuler des arnmi et tant que biomarqueurs d'efficacité d'une thérapie chez les patients souffrant d'arthrite rhumatoïde et traités avec anti-tnfα

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008153692A2 (fr) 2007-05-22 2008-12-18 The Brigham And Women's Hospital, Inc. Établissement de profil d'expression de micro arn du fluide cérébrospinal
WO2010135570A1 (fr) * 2009-05-20 2010-11-25 Board Of Regents, The University Of Texas System Identification de micro-arn impliqués dans un remodelage post-infarctus du myocarde et une insuffisance cardiaque
WO2010139810A1 (fr) 2009-06-05 2010-12-09 Febit Holding Gmbh Empreinte de micro-arn utilisée dans le diagnostic du cancer du poumon
WO2011163214A2 (fr) 2010-06-21 2011-12-29 Diogenix, Inc. Profils de micro-arn pour une évaluation de la sclérose en plaques
WO2015177368A1 (fr) * 2014-05-22 2015-11-26 Servicio Andaluz De Salud Procédé pour faire circuler des arnmi et tant que biomarqueurs d'efficacité d'une thérapie chez les patients souffrant d'arthrite rhumatoïde et traités avec anti-tnfα

Non-Patent Citations (42)

* Cited by examiner, † Cited by third party
Title
"Applied Biosystems Catalogue", vol. 39345, 1 June 2008, APPLIED BIOSYSTEMS, USA, article APPLIED BIOSYSTEMS: "TaqMan Gene Signature Arrays TaqMan Human MicroRNA Arrays", pages: 1 - 2, XP055343551 *
A. J. TIJSEN ET AL: "Circulating microRNAs as diagnostic biomarkers for cardiovascular diseases", AMERICAN JOURNAL OF PHYSIOLOGY: HEART AND CIRCULATORY PHYSIOLOGY, vol. 303, no. 9, 31 August 2012 (2012-08-31), US, pages H1085 - H1095, XP055331266, ISSN: 0363-6135, DOI: 10.1152/ajpheart.00191.2012 *
ABELA GS; AZIZ K; VEDRE A; PATHAK DR; TALBOTT JD; DEJONG J: "Effect of cholesterol crystals on plaques and intima in arteries of patients with acute coronary and cerebrovascular syndromes", AM J CARDIOL., vol. 103, 2009, pages 959 - 68, XP026031829, DOI: doi:10.1016/j.amjcard.2008.12.019
AMENTO EP; EHSANI N; PALMER H; LIBBY P: "Cytokines and growth factors positively and negatively regulate interstitial collagen gene expression in human vascular smooth muscle cells", ARTERIOSCLER THROMB., vol. 11, 1991, pages 1223 - 30
ANDREOU IOANNIS ET AL: "miRNAs in atherosclerotic plaque initiation, progression, and rupture", TRENDS IN MOLECULAR MEDICINE, ELSEVIER CURRENT TRENDS, GB, vol. 21, no. 5, 11 March 2015 (2015-03-11), pages 307 - 318, XP029585941, ISSN: 1471-4914, DOI: 10.1016/J.MOLMED.2015.02.003 *
ANNA P PILBROW ET AL: "Elevated Levels of Circulating MicroRNA-223, MicroRNA-127 and MicroRNA-146a are Associated with Risk of Near Future Cardiovascular Events in Asymptomatic Individuals | Circulation", CIRCULATION, 25 November 2014 (2014-11-25), XP055342174, Retrieved from the Internet <URL:http://circ.ahajournals.org/content/130/Suppl_2/A18066> [retrieved on 20170203] *
BARTEL DP: "MicroRNAs: genomics, biogenesis, mechanism, and function", CELL, vol. 116, 2004, pages 281 - 97, XP002359089, DOI: doi:10.1016/S0092-8674(04)00045-5
CHRISTIAN SCHULTE ET AL: "microRNA-based diagnostics and therapy in cardiovascular disease-Summing up the facts", CARDIOVASCULAR DIAGNOSIS AND THERAPY, 1 February 2015 (2015-02-01), pages 17 - 36, XP055284201, Retrieved from the Internet <URL:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329169/pdf/cdt-05-01-017.pdf> [retrieved on 20160628], DOI: 10.3978/j.issn.2223-3652.2014.12.03 *
CHRISTIAN SCHULTE ET AL: "miRNA-197 and miRNA-223 Predict Cardiovascular Death in a Cohort of Patients with Symptomatic Coronary Artery Disease", PLOS ONE, vol. 10, no. 12, 31 December 2015 (2015-12-31), pages e0145930, XP055342590, DOI: 10.1371/journal.pone.0145930 *
CREA F; LIUZZO G.: "Pathogenesis of acute coronary syndromes", J AM COLL CARDIOL., vol. 61, 2013, pages 1 - 11, XP055230220, DOI: doi:10.1016/j.jacc.2012.07.064
DE RONDE M W J ET AL: "MicroRNAs as biomarkers for atherosclerotic plaque instability", EUROPEAN HEART JOURNAL, OXFORD UNIVERSITY PRESS, GB, vol. 37, no. Suppl.1, 1 August 2016 (2016-08-01), pages 1404, XP009193391, ISSN: 0195-668X *
ERBEL R; O'NEILL W; AUTH D; HAUDE N; NIXDORF U; RUPPRECHT HJ; DIETZ U; MEYER J: "Highfrequency rotablation of occluded coronary artery during heart catheterization", CATHET CARDIOVASC DIAGN, vol. 17, 1989, pages 56 - 8
GOETTSCH C; RAUNER M; PACYNA N; HEMPEL U; BORNSTEIN SR; HOFBAUER LC: "miR-125b regulates calcification of vascular smooth muscle cells", AM J PATHOL., vol. 179, 2011, pages 1594 - 600
GUPTA SK; BANG C; THUM T: "Circulating microRNAs as biomarkers and potential paracrine mediators of cardiovascular disease", CIRC CARDIOVASC GENET, vol. 3, 2010, pages 484 - 8, XP055237788, DOI: doi:10.1161/CIRCGENETICS.110.958363
HANSSON GK: "Inflammation, atherosclerosis, and coronary artery disease", N ENGL J MED., vol. 352, 2005, pages 1685 - 95, XP003014526, DOI: doi:10.1056/NEJMra043430
HUANG S; CHEN M; LI L; HE M; HU D; ZHANG X; LI J; TANGUAY RM; FENG J; CHENG L: "Circulating MicroRNAs and the occurrence of acute myocardial infarction in Chinese populations", CIRC CARDIOVASC GENET, vol. 7, 2014, pages 189 - 98
JINGYI REN ET AL: "Signature of Circulating MicroRNAs as Potential Biomarkers in Vulnerable Coronary Artery Disease", PLOS ONE, vol. 8, no. 12, 5 December 2013 (2013-12-05), pages e80738, XP055342656, DOI: 10.1371/journal.pone.0080738 *
JOVANOVIC IVAN ET AL: "The co-inertia approach in identification of specific microRNA in early and advanced atherosclerosis plaque", MEDICAL HYPOTHESES, EDEN PRESS, PENRITH, US, vol. 83, no. 1, 23 April 2014 (2014-04-23), pages 11 - 15, XP029026410, ISSN: 0306-9877, DOI: 10.1016/J.MEHY.2014.04.019 *
JUN WANG ET AL: "miR-223 Inhibits Lipid Deposition and Inflammation by Suppressing Toll-Like Receptor 4 Signaling in Macrophages", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2012 MDPI AG CHE, vol. 16, no. 10, 20 October 2015 (2015-10-20), pages 24965 - 24982, XP055341804, ISSN: 1661-6596, DOI: 10.3390/ijms161024965 *
KOK MG; HALLIANI A; MOERLAND PD; MEIJERS JC; CREEMERS EE; PINTO-SIETSMA SJ: "Normalization panels for the reliable quantification of circulating microRNAs by RT-qPCR", FASEBJ, vol. 29, 2015, pages 3853 - 62, XP055226125, DOI: doi:10.1096/fj.15-271312
KOVACIC JASON C: "Unlocking the Many Secrets of Noncoding RNA", JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, vol. 65, no. 23, 16 June 2015 (2015-06-16), pages 2538 - 2541, XP029225875, ISSN: 0735-1097, DOI: 10.1016/J.JACC.2015.04.014 *
LIBBY P: "Mechanisms of acute coronary syndromes and their implications for therapy", N ENGL J MED., vol. 368, 2013, pages 2004 - 13
LIBBY P; RIDKER PM; HANSSON GK; LEDUCQ: "Transatlantic Network on A. Inflammation in atherosclerosis: from pathophysiology to practice", J AM COLL CARDIOL., vol. 54, 2009, pages 2129 - 38
M. G. M. KOK ET AL: "Normalization panels for the reliable quantification of circulating microRNAs by RT-qPCR", THE FASEB JOURNAL, vol. 29, no. 9, 28 May 2015 (2015-05-28), US, pages 3853 - 3862, XP055226125, ISSN: 0892-6638, DOI: 10.1096/fj.15-271312 *
MACH F; SCHONBECK U; BONNEFOY JY; POBER JS; LIBBY P: "Activation of monocyte/macrophage functions related to acute atheroma complication by ligation of CD40: induction of collagenase, stromelysin, and tissue factor", CIRCULATION, vol. 96, 1997, pages 396 - 9
MICHELL DANIELLE L ET AL: "Lipoprotein carriers of microRNAs", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - MOLECULAR AND CELL BIOLOGY OF LIPIDS, vol. 1861, no. 12, 26 January 2016 (2016-01-26), pages 2069 - 2074, XP029795155, ISSN: 1388-1981, DOI: 10.1016/J.BBALIP.2016.01.011 *
MITCHELL PS; PARKIN RK; KROH EM; FRITZ BR; WYMAN SK; POGOSOVA-AGADJANYAN EL; PETERSON A; NOTEBOOM J; O'BRIANT KC; ALLEN A: "Circulating microRNAs as stable blood-based markers for cancer detection", PROC NATL ACAD SCI USA, vol. 105, 2008, pages 10513 - 8, XP055037905, DOI: doi:10.1073/pnas.0804549105
MURAKAMI T; MIZUNO S; TAKAHASHI Y; OHSATO K; MORIUCHI I; ARAI Y; MIFUNE J; SHIMIZU M; OHNAKA M: "Intracoronary aspiration thrombectomy for acute myocardial infarction", AM J CARDIOL, vol. 82, 1998, pages 839 - 44
NAGHAVI ET AL., CIRCULATION, vol. 108, 2003, pages 1664 - 1672
NAGHAVI ET AL., CIRCULATION, vol. 108, 2003, pages 1772 - 1778
NATALIA SIMIONESCU ET AL: "Hyperglycemia Determines Increased Specific MicroRNAs Levels in Sera and HDL of Acute Coronary Syndrome Patients and Stimulates MicroRNAs Production in Human Macrophages", PLOS ONE, vol. 11, no. 8, 12 August 2016 (2016-08-12), pages e0161201, XP055341810, DOI: 10.1371/journal.pone.0161201 *
NISHI H; FISHER EA: "Cholesterol homeostasis regulation by miR-223: basic science mechanisms and translational implications", CIRC RES., vol. 116, 2015, pages 1112 - 4
ROBERT E. FARRELL, JR.: "RNA Methodologies, A laboratory guide for isolation and characterization, 2nd edition", 1998, ACADEMIC PRESS
RUIJTER JM; RAMAKERS C; HOOGAARS WM; KARLEN Y; BAKKER O; VAN DEN HOFF MJ; MOORMAN AF: "Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data", NUCLEIC ACIDS RES., vol. 37, 2009, pages e45
SCHULTE C; MOLZ S; APPELBAUM S; KARAKAS M; OJEDA F; LAU DM; HARTMANN T; LACKNER KJ; WESTERMANN D; SCHNABEL RB: "miRNA-197 and miRNA-223 Predict Cardiovascular Death in a Cohort of Patients with Symptomatic Coronary Artery Disease", PLOS ONE, vol. 10, 2015, pages e0145930
SHAN Z; QIN S; LI W; WU W; YANG J; CHU M; LI X; HUO Y; SCHAER GL; WANG S: "An Endocrine Genetic Signal Between Blood Cells and Vascular Smooth Muscle Cells: Role of MicroRNA-223 in Smooth Muscle Function and Atherogenesis", J AM COLL CARDIOL, vol. 65, 2015, pages 2526 - 37, XP029225868, DOI: doi:10.1016/j.jacc.2015.03.570
SUFANG LI ET AL: "MicroRNA-223 inhibits tissue factor expression in vascular endothelial cells", ATHEROSCLEROSIS, vol. 237, no. 2, 18 October 2014 (2014-10-18), AMSTERDAM, NL, pages 514 - 520, XP055342156, ISSN: 0021-9150, DOI: 10.1016/j.atherosclerosis.2014.09.033 *
TANAKA A; SHIMADA K; SANO T; NAMBA M; SAKAMOTO T; NISHIDA Y; KAWARABAYASHI T; FUKUDA D; YOSHIKAWA J: "Multiple plaque rupture and C-reactive protein in acute myocardial infarction", JAM COLL CARDIOL., vol. 45, 2005, pages 1594 - 9, XP027719661
VICKERS KC; LANDSTREET SR; LEVIN MG; SHOUCRI BM; TOTH CL; TAYLOR RC; PALMISANO BT; TABET F; CUI HL; RYE KA: "MicroRNA-223 coordinates cholesterol homeostasis", PROC NATL ACAD SCI U S A., vol. 111, 2014, pages 14518 - 23
YONETSU T; KAKUTA T; LEE T; TAKAHASHI K; KAWAGUCHI N; YAMAMOTO G; KOURA K; HISHIKARI K; LESAKA Y; FUJIWARA H: "In vivo critical fibrous cap thickness for rupture-prone coronary plaques assessed by optical coherence tomography", EUR HEART, vol. 32, 2011, pages 1251 - 9
ZAMPETAKI A; MAYR M: "Analytical challenges and technical limitations in assessing circulating miRNAs", THROMBOSIS AND HAEMOSTASIS, vol. 108, 2012, pages 592 - 8
ZAMPETAKI A; WILLEIT P; TILLING L; DROZDOV I; PROKOPI M; RENARD JM; MAYR A; WEGER S; SCHETT G; SHAH A: "Prospective study on circulating MicroRNAs and risk of myocardial infarction", J AM COLL CARDIOL, vol. 60, 2012, pages 290 - 9, XP028427529, DOI: doi:10.1016/j.jacc.2012.03.056

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021153753A1 (fr) * 2020-01-30 2021-08-05 東レ株式会社 Procédé, dispositif et programme d'examen

Similar Documents

Publication Publication Date Title
US20220033905A1 (en) Method for diagnosis and prognosis of chronic heart failure
Tsai et al. Serum microRNA-21 and microRNA-221 as potential biomarkers for cerebrovascular disease
Olivieri et al. Age-related differences in the expression of circulating microRNAs: miR-21 as a new circulating marker of inflammaging
DK3155120T5 (en) Procedures for diagnosing bone fractures and disorders
Fukushima et al. Assessment of plasma miRNAs in congestive heart failure
Wanhainen et al. Screening of circulating microRNA biomarkers for prevalence of abdominal aortic aneurysm and aneurysm growth
Li et al. MicroRNA-25 inhibits high glucose-induced apoptosis in renal tubular epithelial cells via PTEN/AKT pathway
AU2017213457B2 (en) Micro-RNA biomarkers and methods of using same
Liu et al. Association between peripheral blood cells mitochondrial DNA content and severity of coronary heart disease
Gao et al. Plasma miR-126 and miR-143 as potential novel biomarkers for cerebral atherosclerosis
Liao et al. Exosome-derived MiRNAs as biomarkers of the development and progression of intracranial aneurysms
Xu et al. Characterization of serum miRNAs as molecular biomarkers for acute Stanford type A aortic dissection diagnosis
JP2013528055A (ja) 検出方法
JP2020531023A (ja) 非アルコール性脂肪性肝疾患、非アルコール性脂肪性肝炎、及び／又は肝線維症の非侵襲的診断
Liang et al. A subset of circulating microRNAs is expressed differently in patients with myocardial infarction
Kaneto et al. MicroRNA profiling identifies miR-7-5p and miR-26b-5p as differentially expressed in hypertensive patients with left ventricular hypertrophy
EP2596136B1 (fr) Marqueur constitué d&#39;un microarn du plasma et nouvelle méthode de diagnostic d&#39;un carcinome hépatocellulaire
Ding et al. A polymorphism rs3746444 within the pre‐miR‐499 alters the maturation of miR‐499‐5p and its antiapoptotic function
US20200188356A1 (en) Novel Circular RNA Biomarkers for Heart Failure
CZ2018597A3 (cs) Postpartální epigenetický profil kardiovaskulárních mikroRNA u matek po komplikované graviditě - nové biomarkery kardiovaskulárního rizika
Li et al. RNA-seq identifies circulating miRNAs as potential biomarkers for plaque rupture in patients with ST-segment elevation myocardial infarction
JP2014504881A (ja) 検出方法
Li et al. Modified high-throughput quantification of plasma microRNAs in heparinized patients with coronary artery disease using heparinase
WO2019143828A2 (fr) Biomarqueurs de l&#39;état cardiovasculaire et leurs utilisations
Yang et al. Effect of miR-126 on intracranial aneurysms and its predictive value for rupture of aneurysms.

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17758143

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 17758143

Country of ref document: EP

Kind code of ref document: A1