WO2018000186A1

WO2018000186A1 - Fluorescence scattering optical tomography system and method

Info

Publication number: WO2018000186A1
Application number: PCT/CN2016/087425
Authority: WO
Inventors: 陈昳丽; 付楠; 朱艳春; 李荣茂; 余绍德; 陈鸣闽; 谢耀钦
Original assignee: 中国科学院深圳先进技术研究院
Priority date: 2016-06-28
Filing date: 2016-06-28
Publication date: 2018-01-04

Abstract

Provided are a fluorescence scatter optical tomography system and a method. The system comprises a stage for supporting a specimen implanted with a nanomaterial emitting cold light by X-ray irradiation, and emitting fluorescence by laser irradiation; an X-ray source for emitting X-rays to the specimen on the stage; an X-ray plate detector for acquiring CT imaging of the specimen by X-ray irradiation; EMCCD for acquiring XLCT imaging of the specimen by X-ray irradiation; and a laser for emitting laser light to the specimen, wherein the EMCCD is also used to acquire the laser image and the fluorescent image of the specimen irradiated by the laser light, and the laser image, the fluorescence image and the CT imaging are used for reconstructing FDOT imaging. The imaging system and method can be used to obtain information with more accurate depth, reduce reconstruction difficulty and shorten the data acquisition cycle.

Description

Fluorescence scattering optical tomography system and method

Technical field

The present invention relates to the field of medical imaging technology, and in particular to a fluorescence scattering optical tomography system and method.

Background technique

Compared with MRI, CT and PET, Fluorescence Diffuse Optical Tomography (FDOT) has the advantages of low cost, easy operation and no radiation. It is commonly used in small animal living imaging. The working principle of FDOT technology is to use laser to scan in a certain plane, implant tumors and corresponding targeted fluorescent reagents in small animals in advance, fluorescent reagents are excited by laser, emit near-infrared light, and obtain excitation light through detectors. Image, through accurate three-dimensional reconstruction to determine the location and distribution of tumors in animals. However, the existing FDOT technology is difficult to reconstruct, and the depth information is often inaccurate, and the time period for collecting data is long.

Summary of the invention

Embodiments of the present invention provide a fluorescence scattering optical tomography system for obtaining more accurate depth information, reducing reconstruction difficulty, and shortening a data acquisition period. The fluorescence scattering optical tomography system includes:

a stage for carrying a sample, the sample being implanted with a nano material, the nano material emitting cold light by X-ray irradiation, and emitting fluorescence by laser irradiation;

An X-ray source for emitting X-rays to a sample on the stage;

An X-ray flat panel detector for obtaining CT imaging of the sample by X-ray irradiation;

EMCCD for obtaining XLCT imaging of the sample by X-ray irradiation;

a laser for emitting laser light to the sample;

The EMCCD is also used to obtain laser-irradiated laser images and fluorescent images of the sample, the laser images, fluorescent images, and CT imaging for reconstructing FDOT imaging.

In one embodiment, the stage is a rotating stage; the X-ray flat panel detector is specifically configured to obtain CT imaging of a plurality of angles of rotation of the sample on a rotating stage; the EMCCD is specifically used for XLCT imaging of the plurality of angles at which the sample is rotated on the rotating stage is obtained.

In one embodiment, the fluorescence scattering optical tomography system further comprises:

A micro-displacement stage for controlling laser movement by clamping a fiber optic head of the laser; the EMCCD is specifically for obtaining a plurality of laser images and fluorescent images of the sample irradiated by the moving laser.

a filter disposed between the EMCCD and the stage for filtering the fluorescence emitted by the sample by laser irradiation, so that the EMCCD obtains a laser image of the sample irradiated by the laser; filtering the laser emitted by the laser, The EMCCD was subjected to a laser-irradiated fluorescence image of the sample.

Embodiments of the present invention further provide a fluorescence scattering optical tomography method for obtaining more accurate depth information, reducing reconstruction difficulty, and shortening a data acquisition period. The fluorescence scattering optical tomography method includes:

Placing a sample on a stage, the sample being implanted with a nano material that emits luminescence by X-ray irradiation and emits fluorescence by laser irradiation;

Opening an X-ray source and an X-ray flat panel detector, the X-ray source emitting X-rays to the sample on the stage, and the X-ray flat panel detector obtains CT imaging of the sample by X-ray irradiation; the EMCCD obtains the sample by X-ray irradiation of XLCT imaging;

Turning off the X-ray source and the X-ray flat panel detector, turning on the laser, and the laser emitting laser light to the sample; the EMCCD obtains the laser image and the fluorescent image of the sample irradiated by the laser;

According to the CT imaging, the laser image, and the fluorescence image, FDOT imaging is reconstructed.

In one embodiment, the stage is a rotating stage; the sample is rotated on a rotating stage;

An X-ray flat panel detector obtains CT imaging of the sample by X-ray irradiation, comprising: an X-ray flat panel detector obtaining CT images of the plurality of angles of rotation of the sample on the rotating stage;

The EMCCD obtains X-ray illuminated XLCT imaging of the sample, including: EMCCD obtains multiple angles of XLCT imaging of the sample rotating on a rotating stage.

In one embodiment, the EMCCD obtains X-ray illuminated XLCT imaging of the sample, including:

Solving the scattering equation by finite element method

Obtain the matrix equation M·φ(r)=F·ε·X(r)·ρ, and then solve it by sparse matrix normalization method.

Minimize the problem, get ρ; reconstruct XLCT imaging according to ρ;

Where r is the position; D(r) is the diffusion coefficient, D(r) = (3(μ _a (r) + (1 - g) μ _s (r))) ^-1 ; μ _a (r) is the absorption Coefficient; μ _s (r) is the scattering coefficient; g is the anisotropic parameter; φ(r) is the fluorescence intensity; S(r) is the light source;

M is the photon density; F is the diffusion coefficient of light divergence; ε is the optical field of view; X(r) is the X-ray intensity; ρ is the absorption coefficient of light divergence;

A = (M ^{- 1} F) · ε · X (r); Φ = A · ρ; λ is a normalized parameter.

In one embodiment, the fluorescence scattering optical tomography method further comprises: the micro-displacement station controls laser movement by clamping a fiber tip of the laser;

The EMCCD obtains a laser-irradiated laser image and a fluorescent image of the sample, including: EMCCD obtains a plurality of laser images and fluorescent images of the sample irradiated by the moving laser.

In one embodiment, the EMCCD obtains laser-irradiated laser images and fluorescent images of the sample, including:

A filter is placed between the EMCCD and the stage to filter out the fluorescence emitted by the sample by laser irradiation, and the EMCCD obtains a laser image of the sample irradiated by the laser;

The filter is replaced, the laser light emitted by the laser is filtered out, and the EMCCD obtains a fluorescent image of the sample irradiated with laser light.

In one embodiment, FDOT imaging is reconstructed based on the CT imaging, laser image, and fluorescence image, including:

Obtaining body surface information of the sample according to the CT imaging;

The FDOT imaging is reconstructed based on the body surface information of the sample, the position information of the EMCCD, the sample and the laser, and the laser image and the fluorescence image.

In the embodiment of the invention, the sample is implanted into the nano material, and the nano material emits cold light by X-ray irradiation, and emits fluorescence by laser irradiation; the X-ray source emits X-rays to the sample, and the X-ray flat panel detector obtains the X-ray irradiated sample by the X-ray flat panel detector. Imaging, EMCCD obtains X-ray XLCT imaging of the sample; laser emits laser light to the sample, EMCCD obtains laser-irradiated laser image and fluorescence image of the sample; laser image, fluorescence image and CT imaging are used to reconstruct FDOT imaging, and FDOT imaging system Combined with CT and XLCT imaging systems, CT, XLCT and FDOT imaging can be completed in a short time, shortening the data acquisition period, and can make up for the deficiencies of FDOT in depth information, obtain more accurate depth information, and reduce reconstruction difficulty.

DRAWINGS

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the present invention. Other drawings may also be obtained from those of ordinary skill in the art in light of the inventive work. In the drawing:

1 is a schematic view of a fluorescence scattering optical tomography system according to an embodiment of the present invention;

2 is a schematic diagram of a CT imaging system exploded by a fluorescence scattering optical tomography system according to an embodiment of the present invention;

3 is a schematic diagram of an XLCT imaging system exploded by a fluorescence scattering optical tomography system according to an embodiment of the present invention;

4 is a schematic diagram of an FDOT imaging system exploded by a fluorescence scattering optical tomography system according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of a method of fluorescence scattering optical tomography according to an embodiment of the present invention.

Detailed ways

The embodiments of the present invention will be further described in detail below with reference to the accompanying drawings. The illustrative embodiments of the present invention and the description thereof are intended to explain the present invention, but are not intended to limit the invention.

In order to obtain more accurate depth information, reduce reconstruction difficulty, and shorten data collection period, embodiments of the present invention provide a fluorescence scattering optical tomography system that combines FDOT imaging technology with CT (Computed Tomography). Tomography) XLCT (X-ray Luminescence Computed Tomography) imaging technology is spatially fused, and FDOT imaging is combined with CT and XLCT imaging to compensate for the lack of depth information in FDOT imaging. Therefore, accurate reconstruction of tumor distribution depth information is obtained after reconstruction, which greatly improves imaging quality, reduces reconstruction difficulty, and shortens data collection period. CT imaging is used as a priori information for FDOT imaging data acquisition. The FDOT system is integrated with the CT imaging system, which enables the functional images provided by the FDOT imaging system to be compared and processed by CT imaging, with the aid of CT imaging. The spatial structure is presented more intuitively and accurately. The XLCT imaging system and the FDOT imaging system are spatially fused to obtain accurate prior information and fluorescence information.

1 is a schematic diagram of a fluorescence scattering optical tomography system in accordance with an embodiment of the present invention. As shown in FIG. 1, the fluorescence scattering optical tomography system in the embodiment of the present invention may include:

a stage for carrying a sample, the sample being implanted with a nano material, the nano material being luminescence by X-ray irradiation, and emitting fluorescence by laser irradiation;

An X-ray source for emitting X-rays to a sample on the stage;

X-ray flat panel detector for obtaining CT image of X-ray irradiation of the sample;

EMCCD for obtaining XLCT imaging of X-rays of samples;

a laser for emitting laser light to the sample;

The EMCCD is also used to obtain laser-irradiated laser images and fluorescence images of samples, laser images, fluorescence images, and CT imaging for reconstruction of FDOT imaging.

It can be known from the structure shown in FIG. 1 that the fluorescence scattering optical tomography system of the embodiment of the present invention adopts a method of combining an FDOT imaging system with a CT imaging system and an XLCT imaging system, and completes XLCT, FDOT, and CT in a short time. Imaging, fusion of CT imaging, XLCT imaging and FDOT imaging. As shown in Figure 2-4, The fluorescence scattering optical tomography system can be decomposed into a CT imaging system, an XLCT imaging system, and an FDOT imaging system.

2 is a schematic diagram of a CT imaging system exploded by a fluorescence scattering optical tomography system according to an embodiment of the present invention. As shown in Figure 2, the exploded CT imaging system includes an X-ray source and an X-ray flat panel detector. The X-ray is emitted by the X-ray source, the X-ray flat panel detector detects the X-ray signal, and the CT image is reconstructed.

3 is a schematic diagram of an XLCT imaging system exploded by a fluorescence scattering optical tomography system according to an embodiment of the present invention. As shown in Figure 3, the exploded XLCT imaging system includes an X-ray source and an EMCCD (CCD camera). A filter is also included in FIG. The X-ray source emits X-rays to the sample, the nano-materials in the sample are luminescence by X-ray irradiation, and the EMCCD collects luminescence to reconstruct XLCT imaging. XLCT technology is a hot topic in current scientific research, opening up new possibilities for X-ray molecular imaging. In XLCT imaging systems, nanomaterials can excite near-infrared light under X-rays, and because X-rays and near-infrared light have long penetrability in tissues, they are well suited for imaging in vivo. In the XLCT imaging system, the tomographic image is obtained from a series of X-ray excitations and is obtained by a highly sensitive CCD (Charge-Coupled Device). Recently, a large number of studies have focused on improving biological imaging through the properties of nanomaterials.

4 is a schematic diagram of an FDOT imaging system exploded by a fluorescence scattering optical tomography system according to an embodiment of the present invention. As shown in Figure 4, the exploded FDOT imaging system includes a laser and an EMCCD. The laser emits a laser to the sample, and the nanomaterial in the sample is irradiated with laser light to emit fluorescence, and the EMCCD obtains a laser image and a fluorescent image of the collected sample. Also shown in Figure 4 is a collimator that may be included, the laser being a near infrared laser.

In a specific implementation, the stage can be a rotating stage, and the X-ray flat panel detector can obtain CT imaging of the plurality of angles of the sample rotating on the rotating stage, and the EMCCD can obtain multiple angles of the sample rotating on the rotating stage. XLCT imaging. For example, in the exploded XLCT imaging system shown in Figure 3, the nanomaterial in the sample is excited by the X-ray source to emit luminescence, the sample is rotated on the stage, and the EMCCD obtains luminescence imaging of the sample at various angles.

In a specific implementation, the fluorescence scattering optical tomography system of the embodiment of the present invention may further include: a micro-displacement stage for controlling laser movement by clamping a fiber head of the laser; and the EMCCD is specifically used for obtaining a laser beam irradiated by the sample. Laser images and fluorescent images.

In a specific implementation, the fluorescence scattering optical tomography system of the embodiment of the present invention may further include: a filter disposed between the EMCCD and the stage for filtering the fluorescence emitted by the sample by the laser, so that the EMCCD obtains the sample. Laser-irradiated laser image; filtering out the laser from the laser, allowing EMCCD to obtain a laser-irradiated image of the sample.

For example, in the exploded FDOT imaging system shown in FIG. 4, a laser, a micro-displacement stage, a stage, a filter, and an EMCCD are included. The fiber optic head of the laser is held by a micro-displacement stage to control laser movement. As shown in Fig. 1, the laser emits laser light from right to left, and the sample is scanned in a plane parallel to the EMCCD. The nano material in the sample is, for example, located in the tumor area of the small animal, and the nano material is excited to be fluorescent and collected by EMCCD. The fluorescence distribution in small animals can be reconstructed by the FDOT reconstruction algorithm.

In the embodiment, the xy plane is assumed to be a horizontal plane, the z-axis is an axis of a vertical horizontal plane, and the EMCCD, the X-ray source, the laser, and the X-ray flat panel detector are in the xy plane. Of course, the EMCCD, the X-ray source, the laser, and the X-ray flat panel detector are not It is limited to the xy plane and can be in a certain plane. Here, the xy plane is taken as an example for description. A laser fiber head is mounted on a two-dimensional micro-displacement stage, and the laser light is incident on the object on the opposite plane of the EMCCD, that is, the xz plane. The laser fiber moves along a set position in a plane, and the movement manner can be various. For example, the position of the laser scanning can be moved by a certain position along the x-axis at a certain distance, and moved N times; the z-axis is at a certain distance. Move one position, move N times, form an array of lasers - (N+1) × (N + 1) matrix; or you can use a method that moves a position at a certain angle along the circumference centered on a certain point. The EMCCD filters the laser and fluorescence through filters, and collects fluorescent images from the body and images that are lasered onto the object. The FDOT three-dimensional reconstruction is performed by the reconstruction algorithm to obtain accurate position information such as the fluorescence distribution in the object.

FIG. 5 is a schematic diagram of a method of fluorescence scattering optical tomography according to an embodiment of the present invention. As shown in FIG. 5, the fluorescence scattering optical tomography method may include:

Step 501: placing a sample on the stage, the sample is implanted into the nano material, and the nano material emits cold light by X-ray irradiation, and emits fluorescence by laser irradiation;

Step 502: Open an X-ray source and an X-ray flat panel detector, the X-ray source emits X-rays to the sample on the stage, the X-ray flat panel detector obtains the CT image of the sample by X-ray irradiation; and the EMCCD obtains the sample by X-ray irradiation. XLCT imaging;

Step 503: Turn off the X-ray source and the X-ray flat panel detector, turn on the laser, and the laser emits laser light to the sample; the EMCCD obtains the laser image and the fluorescent image of the sample irradiated by the laser;

Step 504: reconstructing FDOT imaging according to CT imaging, laser image, and fluorescence image.

In a specific implementation, the stage may be a rotating stage; the sample is rotated on the rotating stage; the X-ray flat panel detector obtains the CT image of the sample by X-ray irradiation, which may include: the X-ray flat panel detector obtains the sample in the rotation CT imaging of multiple angles of rotation of the stage; EMCCD obtains X-ray XLCT imaging of the sample, which may include: EMCCD obtains multiple angles of XLCT imaging of the sample rotating on the rotating stage.

In a specific implementation, the fluorescence scattering optical tomography method may further include: the micro-displacement station controls the laser movement by clamping the fiber head of the laser; and the EMCCD obtains the laser-irradiated laser image and the fluorescence image of the sample, which may include: obtaining the sample by the EMCCD A plurality of laser images and fluorescent images of the moving laser light.

In the specific implementation, the EMCCD obtains the laser image and the fluorescence image of the sample, which may include: placing a filter between the EMCCD and the stage, filtering out the fluorescence emitted by the sample by laser irradiation, and obtaining the sample by laser irradiation by EMCCD. The laser image; the filter is replaced, the laser from the laser is filtered out, and the EMCCD obtains a fluorescent image of the sample after laser irradiation.

A specific example is given below to illustrate the working process of the fluorescence scattering optical tomography method in the embodiment of the present invention. The working process in this example may include:

1. First place the object to be reconstructed on the rotating stage. The object contains some kind of nano material. The nano material can emit near-infrared fluorescence under the corresponding excitation light source, and can emit cold light under X-ray to adjust the EMCCD field of view. To cover the entire object;

2. Open the X-ray source and the X-ray flat panel detector;

3. Rotating the rotating stage at a certain speed, and collecting the sample image by the X-ray flat panel detector to reconstruct the CT image of the sample;

4. At the same time, the cold light emitted from the sample is collected by EMCCD, and the XLCT image is obtained by the XLCT reconstruction algorithm;

5. Turn off the X-ray source and the X-ray flat panel detector;

6. Turn on the laser;

7. operating a two-dimensional micro-displacement stage holding the laser fiber to move in a preset manner, that is, moving the position of the laser fiber head to emit laser light at different positions;

8. Put a filter on the EMCCD to filter out the fluorescence emitted by the object. Only the laser image is collected. For example, the object is excited at 488 nm, and the fluorescence is emitted from 600 nm to 700 nm. The first filter is 488 nm narrow band pass. With 10nm) filter, only EMCCD collects 488nm light;

9. Change the filter and collect the fluorescence image. For example, the object is excited at 488 nm, emits fluorescence from 600 nm to 700 nm, and the filter is changed to a long pass filter of 600 nm or higher, so that the EMCCD collects the fluorescent image;

10. Fluorescence and laser images acquired by CT imaging and EMCCD are used as input files of FDOT to reconstruct FDOT imaging.

In specific implementation, the EMCCD obtains XLCT imaging of the sample by X-ray irradiation, which may include:

Solving the scattering equation by finite element method

Minimize the problem, get ρ; reconstruct XLCT imaging according to ρ;

A = (M ^{- 1} F) · ε · X (r); Φ = A · ρ; λ is a normalized parameter.

The process and principle of reconstructing XLCT imaging in the XLCT imaging system are detailed below:

X-rays are emitted from the X-ray source and pass through the object being detected. When an X-ray passes through an object, the object emits near-infrared light as in equation (1):

S(r)=εX(r)ρ(r) (1)

Where r is the position, S(r) is the light source, X(r) is the X-ray intensity, ρ(r) is the nano-optical intensity, and ε is the optical field of view.

According to the lambert-beers criterion, when X-rays pass through an object, the X-ray intensity distribution is as follows:

Where X ₀ is the X-ray intensity at the original position r ₀ and μ _t (τ) is the attenuation coefficient of the X-ray at the position τ. The X-ray intensity X(r) is calculated according to the formula (2).

The model of light in biological soft tissue can be obtained by the scattering equation. Because of the high scattering and low absorption of soft tissue in the near infrared field, the transport equation can be expressed as:

Where D(r) is the diffusion coefficient, D(r)=(3(μ _a (r)+(1-g)μ _s (r))) ^-1 ; μ _a (r) is the absorption coefficient; μ _s (r) is the scattering coefficient; g is the anisotropic parameter; φ(r) is the fluorescence intensity.

The finite element method is widely used to solve the scattering equation. According to the finite element theory, the following matrix equation can be obtained:

M·φ(r)=F·ε·X(r)·ρ (4)

Where M is the photon density; F is the diffusion coefficient of light divergence; ε is the optical field of view; X(r) is the X-ray intensity; and ρ is the absorption coefficient of light divergence.

Based on the above optical transmission equation, the light emitted from the surface of the object reconstructs the 3D distribution of the X-ray luminescence within the object. Reconstruction is a difficult problem because of the high scattering of light in living tissues. The small timing of collecting data will result in a large number of reconstruction problems.

Because the matrix M is finite in equation (4);

Φ=A·ρ (5)

Here, A = (M ^{- 1} F) · ε · X (r).

Equation (5) establishes a linear relationship between sample distribution and near-infrared detection. The reconstruction of the X-ray luminescent sample is to repair the intensity of the X-ray luminescent sample and the intensity of the collected fluorescence. By combining the XLCT imaging system with the FDOT imaging system, and simultaneously acquiring fluorescence information and near-infrared luminescence information, the image can be matched at various angles (space, time) to compensate for the lack of depth information of the FDOT. It is difficult to solve ρ from equation (5) because of the presence of noise in the detected data and the morbidity of the reconstruction. In most biological applications, X-ray luminescence is sparsely distributed in living organisms, so sparse normalization can be used to solve this problem by minimizing ρ:

λ is a normalized parameter.

After obtaining CT imaging, laser images, and fluorescence images, FDOT imaging can be reconstructed from CT imaging, laser images, and fluorescent images. Specifically, the body surface information of the sample can be obtained according to CT imaging; FDOT imaging is reconstructed according to the body surface information of the sample, the position information of the EMCCD, the sample and the laser, and the laser image and the fluorescence image. For example, firstly, 360-degree imaging information of the sample is obtained by CT imaging, and body surface information of the sample is generated by toastmakemesh; and the fluorescent image and laser image of the sample are obtained through the experimental steps of FDOT imaging, and the positions of the CCD, the sample, and the laser source are combined. Information, as well as body surface information, can be reconstructed to obtain a distribution of fluorescence in the sample. The FDOT reconstruction algorithm can mainly call open source packages such as toast++, wavelet transform, and iso2mesh to complete the entire reconstruction algorithm. Galerkin FEM and zero-order Tikhonov regularization can be used to process the collected sparse fluorescence information matrix.

In summary, in the embodiment of the present invention, the sample is implanted into the nano material, and the nano material emits cold light by X-ray irradiation, and emits fluorescence by laser irradiation; the X-ray source emits X-rays to the sample, and the X-ray flat panel detector obtains the sample. CT imaging of X-ray irradiation, EMCCD obtains X-ray XLCT imaging of the sample; laser emits laser light to the sample, EMCCD obtains laser image and fluorescence image of the sample; laser image, fluorescence image and CT imaging are used to reconstruct FDOT imaging The FDOT imaging system can be combined with CT and XLCT imaging systems to complete CT, XLCT and FDOT imaging in a short time, shorten the data collection period, and can make up for the deficiencies of FDOT in depth information and obtain more accurate depth information. Reduce the difficulty of reconstruction.

Those skilled in the art will appreciate that embodiments of the present invention can be provided as a method, system, or computer program product. Accordingly, the present invention may take the form of an entirely hardware embodiment, an entirely software embodiment, or a combination of software and hardware. Moreover, the invention can take the form of a computer program product embodied on one or more computer-usable storage media (including but not limited to disk storage, CD-ROM, optical storage, etc.) including computer usable program code.

The present invention has been described with reference to flowchart illustrations and/or block diagrams of methods, apparatus (system), and computer program products according to embodiments of the invention. It will be understood that each flow and/or block of the flowchart illustrations and/or FIG. These computer program instructions can be provided to a processor of a general purpose computer, special purpose computer, embedded processor, or other programmable data processing device to produce a machine for the execution of instructions for execution by a processor of a computer or other programmable data processing device. Means for implementing the functions specified in one or more of the flow or in a block or blocks of the flow chart.

The computer program instructions can also be stored in a computer readable memory that can direct a computer or other programmable data processing device to operate in a particular manner, such that the instructions stored in the computer readable memory produce an article of manufacture comprising the instruction device. The apparatus implements the functions specified in one or more blocks of a flow or a flow and/or block diagram of the flowchart.

These computer program instructions can also be loaded onto a computer or other programmable data processing device such that a series of operational steps are performed on a computer or other programmable device to produce computer-implemented processing for execution on a computer or other programmable device. The instructions provide steps for implementing the functions specified in one or more of the flow or in a block or blocks of a flow diagram.

The above described specific embodiments of the present invention are further described in detail, and are intended to be illustrative of the embodiments of the present invention. All modifications, equivalent substitutions, improvements, etc., made within the spirit and scope of the invention are intended to be included within the scope of the invention.

Claims

A fluorescence scattering optical tomography system, comprising:

a stage for carrying a sample, the sample being implanted with a nano material, the nano material emitting cold light by X-ray irradiation, and emitting fluorescence by laser irradiation;

An X-ray source for emitting X-rays to a sample on the stage;

An X-ray flat panel detector for obtaining CT imaging of the sample by X-ray irradiation;

EMCCD for obtaining XLCT imaging of the sample by X-ray irradiation;

a laser for emitting laser light to the sample;

The EMCCD is also used to obtain laser-irradiated laser images and fluorescent images of the sample, the laser images, fluorescent images, and CT imaging for reconstructing FDOT imaging.
A fluorescence scattering optical tomography system according to claim 1, wherein said stage is a rotating stage; said X-ray flat panel detector is specifically for obtaining rotation of said sample on a rotating stage CT imaging at multiple angles; the EMCCD is specifically used to obtain XLCT imaging of multiple angles of rotation of the sample on a rotating stage.
The fluorescence scattering optical tomography system of claim 1 further comprising:

A micro-displacement stage for controlling laser movement by clamping a fiber optic head of the laser; the EMCCD is specifically for obtaining a plurality of laser images and fluorescent images of the sample irradiated by the moving laser.
The fluorescence scattering optical tomography system of claim 1 further comprising:

a filter disposed between the EMCCD and the stage for filtering the fluorescence emitted by the sample by laser irradiation, so that the EMCCD obtains a laser image of the sample irradiated by the laser; filtering the laser emitted by the laser, The EMCCD was subjected to a laser-irradiated fluorescence image of the sample.
A fluorescence scattering optical tomography method, comprising:

Placing a sample on a stage, the sample being implanted with a nano material that emits luminescence by X-ray irradiation and emits fluorescence by laser irradiation;

Opening an X-ray source and an X-ray flat panel detector, the X-ray source emitting X-rays to the sample on the stage, and the X-ray flat panel detector obtains CT imaging of the sample by X-ray irradiation; the EMCCD obtains the sample by X-ray irradiation of XLCT imaging;

Turning off the X-ray source and the X-ray flat panel detector, turning on the laser, and the laser emitting laser light to the sample; the EMCCD obtains the laser image and the fluorescent image of the sample irradiated by the laser;

According to the CT imaging, the laser image, and the fluorescence image, FDOT imaging is reconstructed.
The method of claim 5 wherein said stage is a rotating stage; said sample is rotated on a rotating stage;

An X-ray flat panel detector obtains CT imaging of the sample by X-ray irradiation, comprising: an X-ray flat panel detector obtaining CT images of the plurality of angles of rotation of the sample on the rotating stage;

The EMCCD obtains X-ray illuminated XLCT imaging of the sample, including: EMCCD obtains multiple angles of XLCT imaging of the sample rotating on a rotating stage.
The method of claim 5 wherein the EMCCD obtains X-ray illuminated XLCT imaging of the sample, comprising:

Solving the scattering equation by finite element method
Obtain the matrix equation M·φ(r)=F·ε·X(r)·ρ, and then solve it by sparse matrix normalization method.
Minimize the problem, get ρ; reconstruct XLCT imaging according to ρ;

Where r is the position; D(r) is the diffusion coefficient, D(r) = (3(μ a (r) + (1 - g) μ s (r))) -1 ; μ a (r) is the absorption Coefficient; μ s (r) is the scattering coefficient; g is the anisotropic parameter; φ(r) is the fluorescence intensity; S(r) is the light source;

M is the photon density; F is the diffusion coefficient of light divergence; ε is the optical field of view; X(r) is the X-ray intensity; ρ is the absorption coefficient of light divergence;

A = (M - 1 F) · ε · X (r); Φ = A · ρ; λ is a normalized parameter.
The method of claim 5, further comprising: the micro-displacement stage controls laser movement by clamping a fiber optic head of the laser;

The EMCCD obtains a laser-irradiated laser image and a fluorescent image of the sample, including: EMCCD obtains a plurality of laser images and fluorescent images of the sample irradiated by the moving laser.
The method of claim 5 wherein the EMCCD obtains laser-irradiated laser images and fluorescent images of said sample, comprising:

A filter is placed between the EMCCD and the stage to filter out the fluorescence emitted by the sample by laser irradiation, and the EMCCD obtains a laser image of the sample irradiated by the laser;

The filter is replaced, the laser light emitted by the laser is filtered out, and the EMCCD obtains a fluorescent image of the sample irradiated with laser light.
The method of claim 9 wherein reconstructing the FDOT image based on the CT imaging, the laser image, and the fluorescence image comprises:

Obtaining body surface information of the sample according to the CT imaging;

The FDOT imaging is reconstructed based on the body surface information of the sample, the position information of the EMCCD, the sample and the laser, and the laser image and the fluorescence image.