WO2017215886A1

WO2017215886A1 - Bleach-free powder composition for cleaning hard surfaces

Info

Publication number: WO2017215886A1
Application number: PCT/EP2017/062250
Authority: WO
Inventors: Manoj Vilas Dagaonkar; Srilaxmi Venkata Medepalli; Somnath Ghosh; Rohini Sukumaran NAIR
Original assignee: Unilever N.V.; Unilever Plc; Conopco, Inc., D/B/A Unilever
Priority date: 2016-06-13
Filing date: 2017-05-22
Publication date: 2017-12-21
Also published as: BR112018075064A2; ZA201807624B; BR112018075064B1

Abstract

A powder composition for cleaning toilets, comprising less than 1 % by weight bleach, said composition comprising : • (i) total surfactant content of 1 to 5 wt%, of which at least 70 parts by weight is a linear alkyl benzene sulphonate; • (ii) a first non-phosphate builder (B_f) whose Reserve Alkalinity is < 40 ml of 0.1 N HCI in 1 % aqueous solution; and, • (iii) at least 2 wt% of a second non-phosphate builder (B_s) whose Reserve Alkalinity is > 40 ml of 0.1 N HCI in 1 % aqueous solution, wherein total amount of builder in said composition is 4 to 15 wt%, wherein total amount of builder (B_t) is defined as B_f+ B_s, wherein the composition has a pH of at least 9.5 at 20° C when measured as a 1 % by weight aqueous solution. Method of cleaning with antibacterial efficacy acoording to BS EN 1276 protocol.

Description

BLEACH-FREE POWDER COMPOSITION FOR CLEANING HARD SURFACES Field of the invention The invention relates to a composition for cleaning hard surfaces, more particularly for rendering toilet bowls more hygienic by controlling bacterial growth.

Background of the invention A wide range of compositions is available for cleaning inanimate hard surfaces. Such surfaces would include bathrooms, toilet bowls and kitchens, which are made of a variety of hard materials such as enamel, ceramic, and porcelain.

The types of stains usually encountered in lavatories, toilets and bathrooms include lime scale deposits, soap scum and organic soil. In addition to this, there is significant growth of bacteria in and around toilet bowls.

Compositions that are commercially available for cleaning toilets include acidic cleaners, phenyl and bleach-based cleaners. Some high-end toilet cleaning compositions include gels and pastes.

Acidic compositions are particularly effective against lime scale. Bleach-based compositions are effective against stains. However, these compositions are harsh. WO09085049 A1 (Colgate-Palmolive) discloses a cleaning composition containing an organic acid, an ethoxylated alcohol and a hydrophilic polymer that adsorbs onto hard surfaces.

US2009197786 A (Reckitt Benckiser) discloses hard surface cleaning compositions particularly adapted to clean toilets. The compositions are aqueous, thickened, acidic compositions having acid, a thickening constituent, a detersive surfactant and at least one super-wetter surfactant based on a narrow range ethoxylated alcohol nonionic surfactant having two cloud points. WO12049202A1 (Corbellini) discloses a powdery cleaning agent containing at least one acid, at least one water-soluble carbonate and at least one biocide for cleaning flush toilets and washing machines. US6387868B (Uno Shoyu Co. Ltd, 2002) discloses clear aqueous liquid detergents containing an alkyl benzene sulfonate surfactant, liquid sodium silicate, a polyoxyethylene alkyl or aryl ether nonionic surfactant and an alkyl ether sulfate surfactant. The silicate boosts detergency. This composition is a fabric washing composition, which is dilutable and dissolves in water without becoming cloudy over long period. The presence of nonionic and anionic surfactants provide stability. The pH of these compositions is about 7 to 8.

EP0379093A1 (Sterling Drug Inc, 1990) discloses highly alkaline, yet non-corrosive, aqueous hard surface cleaning compositions for cleaning industrial and kitchen surfaces soiled with burnt-on grease and other organic soils. Combination of alkanolamine to metasilicate in critical ratios along with certain other essential ingredients provide the desired technical effect.

In some parts of the world, people use proxy products to clean their toilets. Such proxy products include low-priced detergent powders. Alternatively, some do use bleaching powders, acids, phenyls or even leftover detergent wash-liquor. While such products do provide some degree of cleaning, they are not prepared to cater to such secondary uses. Therefore, users of such products get sub-optimal cleaning in spite of significant efforts and inconvenience. Further, the users are at risk of exposing themselves to, or coming in contact with human waste, and variety of pathogenic microbes, mainly Gram Positive and Gram Negative bacteria that thrive under unhygienic conditions.

In the absence of proper communication and knowledge of appropriate consumer products, such consumers may remain under the misbelief of clean, hygienic and germ-free toilets.

Therefore, there is need for compositions, which are capable of acting against Gram Positive as well as Gram Negative bacteria within a reasonable time e.g., 10 to 30 minutes of application, without the need to alter the habits of such consumers. The compositions should be bleach-free so that consumer-awareness and product literacy need not become a concern for new users. Further, such products need to be sustainable, utilising less of non-renewable resources like synthetic surfactants.

Romanian patent R01 16484 discloses a cleaning powder comprising 70 to 90 % by weight ground dolomite, 1 to 5 % by weight soda ash, 1 to 5% sodium

tripolyphosphate, and 1 to 5% straight chain sodium salt of alkyl benzene sulfonic acid. The cleaning powder provides stain-free cleaning and is not harsh to human skin.

An abrasive cleaning formulation disclosed in Modern Chemical Specialties (Mac Nair- Dorland Company, 1950) contains 5 % by weight coconut oil soap, 2.5 % by weight sodium silicate, 3.5 % by weight soda ash and 85 % by weight abrasives. However, these formulations are effective against either Gram Positive bacteria or Gram Negative bacteria but not both. Hard surface cleaning compositions, and especially toilet cleaning compositions, should be effective against either form of bacteria. Summary of the invention

We have determined that a solution lies in compositions in accordance with this invention. The compositions are significantly more effective against Gram Positive as well as Gram Negative bacterium.

In accordance with a first aspect is disclosed a bleach-free powder composition for cleaning inanimate hard surfaces, said composition comprising:

(i) total surfactant content of 1 to 5 % by weight, of which at least 70 parts by

weight is non-soap anionic surfactant;

(ii) a first non-phosphate builder (Bf) whose Reserve Alkalinity is < 40 ml of 0.1 N

HCI in 1 % aqueous solution and;

(iii) at least 2 % by weight second non-phosphate builder (B_s) whose Reserve

Alkalinity is > 40 ml of 0.1 N HCI in 1 % aqueous solution, wherein total amount of builder (B_t = Bf + B_s) in said composition is 4 to 15 % by weight.

The invention and all other aspects thereof will be explained in details.

Detailed description of the invention

In the description, the term % by weight denotes the percentage by weight of the composition. For example, the total surfactant content of compositions in accordance with this invention is 1 to 5 % by weight, which means that 100 g of a composition contain 1 to 5 g surfactant.

The term inanimate hard surfaces includes surfaces which are particularly prone to bacterial growth such as floors, walls, tiles, sinks, shower plastified curtains, wash basins, bidets and toilet bowls of Western or Indian-style toilets. On the other hand animate hard surfaces include teeth.

Although the compositions in accordance with this invention are useful for toilet cleaning, the compositions could be used to clean any other inanimate hard surface.

Many people around the world face the problem of unhygienic toilets. It mostly applies to the developing and the least developed countries. Toilet bowls and the adjacent area is prone to bacterial growth. Proxy cleaning products are unable to control their growth. Therefore, the bacteria multiply quickly. Most bacteria that thrive under such conditions are pathogenic.

High-priced products may provide up to 5-log reduction in the viable bacterial count when tested according to European Suspension Test BS EN 1276. However, it is an arduous task to provide such a high level of hygiene, unless the compositions contain significant amount of acid, bleach, antibacterial agents and other active ingredients.

Research indicates that at least 2-log reduction, i.e., 99 % reduction in viable count of bacteria, especially the pathogenic ones, is necessary for minimal hygiene. Gram-positive and Gram-negative bacteria thrive under unhygienic conditions. S. aureus and E.hirae are the most common Gram Positive bacteria whereas P. aeruginosa and E.coli are the most common Gram Negative type. A product of this nature is considered efficacious if it brings about at least 2-log reduction in the viable count of at least one Gram Positive bacteria and at least one Gram Negative bacteria within reasonable time e.g., 10 to 30 minutes. This is broad-spectrum antibacterial activity.

Accordingly, the compositions in accordance with this invention, on contact with an inanimate hard surface comprising thereon at least one Gram positive bacteria selected from S.aureus and E.hirae and at least one Gram Negative bacteria selected from

P.aeruginosa and E.coli; provide, in accordance with BS EN 1276 protocol, at least 2-log reduction in viable count of at least one of the Gram positive bacteria and at least one of the Gram negative bacteria. Compositions in accordance with preferred aspect of this invention provide at least 5-log reduction in the viable count of at least one of each kind when tested according to European Suspension Test BS EN 1276. The EN 1276 Standard describes a quantitative suspension test for the assessment of the bactericidal activity of chemical antiseptics and disinfectants. This test method evaluates how effectively a product causes reduction in number of viable bacterial cells of the relevant test microorganisms.

Consumers prefer hard surface cleaning powders, which are easy to handle and apply. However, some of the expensive detergent compositions contain significant amount of surfactants and it would make little sense to use such expensive products to clean toilets. However, formulating a non-bleach, non-acidic hard surface cleaning composition is a technical problem, especially if the products need to be sustainable and provide at least 2- log reduction in viable bacterial count of at least one Gram Positive bacteria and at least one Gram Negative bacteria. The compositions in accordance with the invention are bleach-free and non-acidic.

By bleach-free is meant that the compositions comprise (cumulatively) less than 1 % by weight bleaching ingredients. Such ingredients include hypochlorites, sulphites, bisulphites, metabisulphites, isocyanurates, persulphates, percarbonates, peroxides, perborates and other bleach ingredients.

Non-acidic composition is defined as a composition having a pH of at least 9.5 measured at 20 °C as a 1 % by weight aqueous solution (i.e. 1 wt% composition in 99 wt% water).

Further, it is preferred that the compositions in accordance with this invention comprise not more than 3 % by weight moisture, as moisture may affect stability. The compositions in accordance with this invention comprise total surfactant content of 1 to 5 % by weight, of which at least 70 parts by weight is non-soap anionic surfactant. For example, when the total surfactant content is 2 % by weight, then at least 1.4 % by weight is non-soap anionic surfactant, which accounts for at least 70 parts by weight of the total surfactants. The total amount of surfactants, which is significantly less than conventional products, contributes to sustainability and reduces the dependence of compositions in accordance with this invention on non-renewable resources.

Surfactant content: The total surfactant content implies the sum total of all surfactants present in the composition in accordance with this invention. The total surfactant content is 1 to 5 % by weight. It is further preferred that the surfactant is composed entirely of non-soap anionic surfactant. The term non-soap anionic surfactant is well known. These surfactants are not soap-based. Soaps are sodium or potassium salts of long chain fatty acid.

It is preferred that the non-soap anionic surfactant is a linear alkyl benzene sulphonate, an alkoxylated primary alcohol sulphate, a non-alkoxylated primary alcohol sulphate, an olefin sulphonate, an ester sulphonate or a secondary alcohol sulphate. Linear alkyl benzene sulphonates have alkyl chain length of Cs-2o. Generally, the counter ion for anionic surfactants is an alkali metal, typically sodium, although instead of alkali metals, other amine based counter ions can also be present. Preferred linear alkyl benzene sulphonate surfactants include sodium salt of linear alkyl benzene sulphonates with an alkyl chain length of from 8 to 15, more preferably 12 to 14.

Alkoxylated primary alcohol sulphates are anionic surfactants generally represented by the general formula RO(C2H40)_xSO3^"M⁺ where R is an alkyl chain having from 10 to 22 carbon atoms, saturated or unsaturated, M is a cation which makes the compound water-soluble, especially an alkali metal, ammonium or substituted ammonium cation, and x averages from 1 to 15. Preferably, R is an alkyl chain having from 12 to 16 carbon atoms, M is sodium and x averages from 1 to 9, preferably x is 1 to 7. It is particularly preferred that the alkoxylated anionic surfactant is sodium lauryl ether sulphate (SLES). The non-alkoxylated primary alcohol sulphates are devoid of any alkoxylated groups. An example is sodium lauryl sulphate.

It is particularly preferred that the non-soap anionic surfactant is linear alkyl benzene sulphonate.

Reference may be made to the surfactants described in "Surface Active Agents" Vol. 1 , by Schwartz & Perry, Interscience 1949, Vol. 2 by Schwartz, Perry & Berch, Interscience 1958, in the current edition of "McCutcheon's Emulsifiers and Detergents" published by Manufacturing Confectioners Company or in "Tenside-Taschenbuch", H. Stache, 2nd Edn., Carl Hauser Verlag, 1981.

It is particularly preferred that compositions in accordance with the invention comprises, cumulatively, less than 1 % by weight surfactant, other than said non-soap anionic surfactant. Such surfactants include soap, non-ionic, cationic and zwitterionic surfactants. This is to prevent, or at least reduce to the extent possible, any unwanted interaction with other ingredients, whenever present.

Builders Compositions in accordance with this invention comprise two different builders. There is a first non-phosphate builder (Bf) whose Reserve Alkalinity is < 40 ml of 0.1 N HCI in 1 % aqueous solution. Persons skilled in the art of home care compositions, especially laundry and hard surface cleaning compositions know the meaning of the term Reserve Alkalinity. It is the amount of 0.1 N HCI necessary to lower the pH to a chosen value, i.e., down to pH 8.

The compositions comprise a second builder in an amount of at least 2 % by weight. The second builder is also a second non-phosphate builder (B_s). Its Reserve Alkalinity is > 40 ml of 0.1 N HCI in 1 % aqueous solution.

The total amount of builder (B_t = Bf + B_s) in the composition is 4 to 15 % by weight.

It is preferred that the amount of the second non-phosphate builder is not more than 6 % by weight.

It is preferred that the first non-phosphate builder (Bf) is a least one of sodium hydroxide or sodium carbonate. It is preferred that the second non-phosphate builder (Bs) is sodium disilicate, sodium silicate, sodium metasilicate, sodium aluminate.

It is particularly preferred that the first non-phosphate builder is sodium carbonate and said second non-phosphate builder is sodium disilicate.

Without wishing to be bound by theory, it is believed that the builders act synergistically against Gram Positive and Gram Negative bacteria. Compositions comprising only one of the builders are effective against only one type of bacteria. While it is possible to increase the amount of one of the builders and remove the other altogether from the formulations, such formulations, though effective, are likely to be unstable, and in particular, may be vulnerable to ingress of moisture, which could render them ineffective.

The presence of the second builder allows for reduction in the total amount of the builders, which makes the compositions more sustainable. Abrasives

It is preferred that compositions in accordance with the invention comprise one or more abrasive whose Mohs Index is at least 3.

This Scale (Index) was developed for minerals to classify according to their hardness, from 1 (the softest) to 10 (the hardest) and has been used by mineralogists since its inception. It is preferred that the abrasive is one or more of calcite, dolomite, sodium sulphate, feldspar, apatite, fluorite, hematite, kyanite, magnetite, orthoclase and pumice. It is particularly preferred that abrasive is entirely calcite or alternatively a combination of calcite and dolomite. It is preferred that abrasive is 15 to 70 % by weight of the composition.

As disclosed above, the compositions in accordance with this invention may, and preferably does comprises two different abrasives, differing in their abrasive index. In such a case, preferably, the compositions comprise a first abrasive having Mohs Index of 3 to 5 and a second abrasive having Mohs Index of 5.5 to 7, collectively amounting to 15 to 70 % by weight of the composition. It is further preferred that the ratio of the amount of the first abrasive having Mohs Index of 3 to 5 to that of the second abrasive having Mohs Index of 5.5 to 7 is in the range of 1 :0.3 to 1 :0.6. It is preferred that the first abrasive is dolomite (Mohs Index 3.5 to 4) and the second abrasive is feldspar (Mohs Index 6 to 6.5). Further preferably, the amount of the first abrasive is 40 to 60 % by weight of the composition. Yet further preferably the amount of the second abrasive is 15 to 30 % by weight of the composition.

Salt

It is preferred that the compositions in accordance with this invention comprise an inorganic salt selected from sodium chloride, potassium chloride, sodium sulphate or potassium sulphate. It is more preferred that the salt is sodium chloride or sodium sulphate. When present, it is preferred that the amount of the salt is 5 to 15 % by weight of the composition.

Use and method in accordance with the invention

In accordance with another aspect is disclosed use of a composition as claimed in claim 1 as an antibacterial toilet cleaner. It is preferred that the composition provides in accordance with BS EN 1276 protocol, at least 2-log reduction in viable count of at least one Gram positive bacteria selected from S.aureus and E.hirae and at least one Gram negative bacteria selected from P.aeruginosa and E.coli. It is further preferred that after application, said composition is allowed contact time of at least 10 to 30 minutes.

In case of using the composition for cleaning toilet bowls, the said composition can be applied to the surface and spread effectively with the help of a scrubber, left for few minutes, e.g., 10 to 30 minutes, and then rinsed with water.

In another aspect the invention provides a method of cleaning an inanimate hard surface comprising thereon at least one Gram positive bacteria selected from S.aureus and E.hirae and at least one Gram Negative bacteria selected from P.aeruginosa and E.coli to bring about at least 2-log reduction in viable count of at least one of said Gram positive bacteria and at least one of said Gram negative bacteria in accordance with BS EN 1276 protocol, said method comprising:

(i) a step of contacting said surface with a composition in accordance with a first aspect of the invention for at least ten minutes; followed by, (ii) washing said surface with water.

Preferably the contact is for 15 to 30 minutes. Optional ingredients: The compositions of the invention may optionally comprise other ingredients, such as fragrance, colorant, foam boosting agents, odour absorbing materials, preservatives (e.g. bactericides), pH buffering agents, anti-oxidants and anti-corrosion agents. Packaging

The composition according to the invention could be packed in any suitable container. Preferably, the compositions are packaged in an air-tight plastic bottle with a detachable closure /pouring spout. The bottle may be rigid or deformable. Preferably, the container is clear enough so that visual cues, like the colour changing actives, if any, are visible from outside. The bottle may be provided with one or more labels, or with a shrink sleeve which is desirably at least partially transparent, for example 50 % of the area of the sleeve is transparent. The adhesive used for any transparent label should preferably not adversely affect transparency. The invention will now be described with reference to the following non-limiting examples.

Examples

Example 1 : Efficacy of compositions outside the invention against Gram Positive bacterium

Compositions in accordance with prior art (MCS and Romanian patent R01 16484) were prepared. Experiments were conducted in order to determine the efficacy of each composition against one Gram Positive bacteria (S.aureus). The efficacy was determined in terms of reduction in the viable bacterial count brought about by the compositions after contact time of 15 minutes as well as 30 minutes.

The procedure is as follows:

The concerned test bacterium was grown overnight at 37 ^°C on a TSA (Tryptic Soya Agar) plate. The grown culture colonies were suspended again in tryptone diluent.

Density of the culture was adjusted to get final count of 1.5-5.0 x 10⁸ cfu/ml based on

McFarland standards (McFarland 1 .5 measured using bioMerieux Densomat®).

Thereafter 240 μΙ of the test solution was placed into test wells in a micro-titre plate.

Equal volumes of test culture and bovine serum albumin (interfering substance) were combined for two minutes, before 60 μΙ of the mixture being added to the test solution. After a contact time of five minutes, 30 μΙ of the mixture was mixed with a neutralising solution. This solution was then serially diluted in tryptone soya broth and then spot- plated onto TSA plates. After absorption, the plates were incubated at 37 ^°C for 24 hours and the residual colonies were counted. All the experiments were done under aseptic conditions and all media were autoclaved at 15 psi pressure before use. The extent of reduction in bacterial count was calculated by taking into account the difference between initial and final cfu/ml values.

Observations are summarised in Table 1 .

Table 1

Example 2: Efficacy of compositions outside the invention against Gram Negative bacterium

Compositions in accordance with prior art (MCS and Romanian patent R01 16484) were prepared. Experiments were conducted in order to determine the efficacy of each formulation against one Gram Negative bacteria {P. aeruginosa). The efficacy was determined in terms of reduction in the viable bacterial count brought about by the compositions after contact time of 15 minutes as well as 30 minutes.

The observations are summarised in Table 2.

Table 2

Log reduction at the end of

Details of the prior art formulation

15 minutes 30 minutes

According to MCS 7.4 7.4

According to R01 16484 0.19 0.21 The data in Tables 1 and 2 collectively indicates that the composition in accordance with the Romanian patent does not provide > 2 log reduction in viable count of either type of bacteria.

On the other hand, the composition in accordance with MCS is effective against only one type of bacteria but not the other. Therefore, it fails to provide broad-spectrum antibacterial action. Example 2: Efficacy of compositions inside the invention and comparative examples

Two compositions inside the scope of the invention (Compositions 1 and 2) were prepared and subjected to experiments to determine their efficacy against S. aureus and P.aeruginosa.

The formulation of each composition is shown in Table 3 and the observations are summarised in Table 4.

Table 3

Table 4

Log reduction at the end of

Details of the Composition & bacterium

5 minutes 15 minutes 30 minutes

Comparative Composition / S. aureus — >5 >5

Comparative Composition / P.aeruginosa — 1.4 1 .4

Composition 1/ S. aureus — >5 >5

Composition 1 / P.aeruginosa — >5 >5

Composition 21 S. aureus >5 >5 >5

Composition 2 / P.aeruginosa >5 >5 >5 The data set out in table 4 clearly indicates synergistic interaction of the ingredients. The comparative composition contains only 1 % of the second non-phosphate builder (sodium disilicate). On the other hand, compositions 1 and 2 contain at least the minimum amount of the second builder necessary for broad-spectrum activity. The data indicates that Compositions 1 and 2 do have antibacterial action whereas the

Comparative Composition (outside the present invention) acts only against one type of bacteria.

Claims

C2301 (V) CPL WO 2017/215886 PCT/EP2017/062250 15 Claims

1 . A bleach-free and non-acidic powder composition for cleaning inanimate hard surfaces, comprising less than 1 % by weight bleaching ingredients, said composition comprising:

(i) total surfactant content of 1 to 5 % by weight, of which at least 70 parts by weight is non-soap anionic surfactant;

(ii) a first non-phosphate builder (Bf) whose Reserve Alkalinity is < 40 ml of 0.1 N HCI in 1 % aqueous solution; and,

(iii) at least 2 % by weight second non-phosphate builder (B_s) whose

Reserve Alkalinity is > 40 ml of 0.1 N HCI in 1 % aqueous solution, wherein total amount of builder in said composition is 4 to 15 % by weight, wherein the total amount of builder (B_t) is defined as Bf + B_s; wherein the composition has a pH of at least 9.5 at 20° C when measured as a 1 % by weight aqueous solution and wherein the non-soap anionic surfactant is a linear alkyl benzene sulphonate.

2. A composition as claimed in claim 1 wherein amount of said second non- phosphate builder is up to 6 % by weight.

3. A composition as claimed in claim 1 or 2 comprising one or more abrasive whose Mohs Index is at least 3.

4. A composition as claimed in any preceding claim 1 to 3 wherein said first non- phosphate builder (Bf) is at least one of sodium hydroxide or sodium carbonate.

5. A composition as claimed in in any preceding claim 1 to 4 wherein said second non-phosphate builder (B_s) is at least one of sodium disilicate, sodium silicate, sodium metasilicate, sodium aluminate.

6. A composition as claimed in any preceding claim 3 to 5 wherein said abrasive is one or more of calcite, dolomite, sodium sulphate, feldspar, apatite, fluorite, hematite, kyanite, magnetite, orthoclase and pumice. C2301 (V) CPL

WO 2017/215886 PCT/EP2017/062250

16

7. A composition as claimed in any preceding claim 1 to 6 wherein total amount of builder (B_t = Bf + B_s) in said composition is 6 to 9 % by weight.

8. A composition as claimed in any preceding claim 1 to 7 wherein said first non- phosphate builder is sodium carbonate and said second non-phosphate builder is sodium disilicate.

9. A method of cleaning an inanimate hard surface comprising thereon at least one Gram positive bacteria selected from S.aureus and E.hirae and at least one Gram Negative bacteria selected from P.aeruginosa and E.coli to bring about at least 2- log reduction in viable count of at least one of said Gram positive bacteria and at least one of said Gram negative bacteria in accordance with BS EN 1276 protocol, said method comprising:

(i) a step of contacting said surface with a composition as claimed in any

preceding claim 1 to 8 for at least ten minutes; followed by,

(ii) washing said surface with water.

10. A composition as claimed in any preceding claim 1 to 8 wherein on contact with an inanimate hard surface comprising thereon at least one Gram positive bacteria selected from S.aureus and E.hirae and at least one Gram Negative bacteria selected from P.aeruginosa and E.coli; said composition provides, in accordance with BS EN 1276 protocol, at least 2-log reduction in viable count of at least one of said Gram positive bacteria and at least one of said Gram negative bacteria.

11. Use of a composition as claimed in any preceding claim 1 to 8 as an antibacterial toilet cleaner.